Standard name
Human Ortholog
Description Profilin; binds actin, phosphatidylinositol 4,5-bisphosphate, and polyproline regions; involved in cytoskeleton organization; required for normal timing of actin polymerization in response to thermal stress; protein abundance increases in response to DNA replication stress; highly conserved protein; human PFN1 (profilin 1) complements temperature sensitive pfy1 mutants, PFN1 mutations are a rare cause of ALS
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 1.0 0.98 0.97 0.99 0.88 0.93 – 0.9 0.87 0.88 0.88 1.0 1.0 1.0 0.99 0.99 0.97 0.96 0.96 0.94 0.98 0.97 0.95
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0.06 0.05 0.08 0 0 0 0.06 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0.08 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 1 2 2 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 2 0 – 0 0 0 1 1 2 0 0 0 1 0 0 0 1 0 3
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 2 3 1 1 7 – 5 6 12 7 1 2 1 0 1 0 0 0 0 0 0 0
Cytoplasm 245 194 98 145 216 126 210 – 165 171 183 181 236 401 452 191 174 146 229 180 104 212 330 367
Endoplasmic Reticulum 1 0 0 2 0 3 9 – 8 12 11 16 0 0 2 11 5 4 0 0 1 0 1 3
Endosome 0 0 0 0 0 0 0 – 1 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0
Golgi 0 0 0 1 0 0 0 – 1 3 2 3 0 0 0 1 1 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 3 0 – 3 0 3 1 0 0 0 0 1 0 0 0 1 0 0 1
Nucleus 0 0 0 0 0 3 4 – 6 3 1 4 0 0 0 0 0 0 2 3 0 0 0 1
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Vac/Vac Membrane 1 0 2 1 4 12 6 – 2 7 2 2 0 0 2 1 0 3 2 1 0 0 1 1
Unique Cell Count 247 194 100 149 219 143 225 183 196 208 206 236 401 454 192 175 151 240 188 111 218 342 387
Labelled Cell Count 247 195 102 152 221 150 236 192 203 216 217 238 405 457 204 182 156 240 188 111 218 342 387


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 58.8 55.8 47.1 43.9 50.5 29.3 42.5 – 32.8 37.2 35.8 38.4 64.2 69.5 70.6 78.8 76.6 76.1 55.9 59.2 63.6
Std Deviation (1e-4) 10.8 10.2 9.2 10.4 11.0 12.0 13.5 – 13.0 12.9 12.0 11.8 13.9 15.2 14.1 29.6 30.0 26.9 9.6 12.2 14.9
Intensity Change (Log2) – – – -0.1 0.1 -0.68 -0.15 – -0.52 -0.34 -0.4 -0.29 0.45 0.56 0.58 0.74 0.7 0.69 0.25 0.33 0.43


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.3 0.4 -2.8 -1.7 – -2.5 -3.0 -2.9 -2.9 2.2 2.8 1.7 1.2 1.1 -0.6
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 2.9 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 2.1 0 – 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 46.1918 58.9161 53.5428 48.9236 48.2352 57.031 66.2489 82.5057 71.0102 69.1326 72.6498 78.5136 61.8494 78.161 67.7208 70.2413 64.626 69.2335
Actin 0.0163 0.0001 0.005 0.0005 0.0001 0.0003 0.0052 0 0.0137 0.0002 0.0055 0.0001 0.0067 0 0.012 0.0008 0 0.0001
Bud 0.0006 0 0.0003 0.004 0.0008 0.0001 0.0002 0 0.0003 0.0001 0.0005 0.0001 0.0002 0 0.0008 0 0 0.0001
Bud Neck 0.0006 0.0001 0.0003 0.0003 0.0004 0.0005 0.0006 0 0.0003 0.0001 0.0002 0.0003 0.001 0 0.0005 0.0002 0.0001 0.0002
Bud Periphery 0.0004 0 0.0002 0.0017 0.0004 0 0.0002 0 0.0003 0 0.0005 0.0003 0.0001 0 0.0004 0 0 0
Bud Site 0.0007 0 0.0004 0.0003 0.0001 0 0.0035 0 0.0005 0.0002 0.0005 0.0001 0.0008 0 0.0019 0.0001 0 0
Cell Periphery 0.0004 0 0.0001 0.0002 0.0001 0 0.0002 0 0.0002 0 0.0002 0.0002 0.0001 0 0.0001 0.0001 0 0
Cytoplasm 0.8503 0.9872 0.9513 0.9076 0.9629 0.9496 0.9334 0.9932 0.9387 0.9529 0.8948 0.9518 0.916 0.9936 0.9253 0.9575 0.985 0.9513
Cytoplasmic Foci 0.0115 0.0001 0.0046 0.0159 0.0008 0.0001 0.0015 0 0.0068 0.0122 0.0053 0.0003 0.013 0 0.0059 0.0005 0.0001 0.002
Eisosomes 0.0002 0 0.0001 0.0001 0 0 0.0001 0 0.0002 0.0001 0.0002 0 0 0 0.0001 0.0002 0 0
Endoplasmic Reticulum 0.0022 0.0001 0.0006 0.0005 0.0004 0.0005 0.0005 0 0.0015 0.0001 0.0005 0.0001 0.0014 0 0.0016 0.0042 0 0.0004
Endosome 0.0046 0 0.0013 0.001 0.0015 0.0001 0.0012 0 0.0015 0.0004 0.0073 0.0003 0.0031 0 0.0057 0.0014 0 0.0024
Golgi 0.0018 0 0.0012 0.0004 0 0 0.0007 0 0.0007 0.0002 0.0057 0 0.0022 0 0.0018 0.0001 0 0
Lipid Particles 0.0048 0 0.0009 0.0087 0 0 0.0012 0 0.0042 0.0037 0.0111 0.0003 0.0044 0 0.0018 0.0006 0 0.0002
Mitochondria 0.0053 0 0.0023 0.0052 0.0005 0.0001 0.0013 0 0.008 0.0011 0.0327 0.0058 0.0014 0 0.004 0.0001 0 0.0002
None 0.0078 0.0001 0.0038 0.007 0.0004 0.0001 0.0011 0 0.0029 0.0021 0.0035 0.0003 0.0039 0.0001 0.0066 0.0213 0.0001 0.0011
Nuclear Periphery 0.0148 0.0013 0.0026 0.0031 0.0031 0.007 0.0068 0.0004 0.0024 0.0016 0.0032 0.0016 0.0039 0.0003 0.0082 0.0027 0.0014 0.0038
Nucleolus 0.0026 0 0.0008 0.0027 0.0001 0.0001 0.0015 0 0.0008 0.002 0.0043 0.0095 0.0027 0 0.0013 0.0002 0 0.0002
Nucleus 0.0556 0.0107 0.0211 0.0319 0.0152 0.0404 0.0362 0.0061 0.0125 0.0193 0.0122 0.0238 0.0333 0.0057 0.0131 0.0086 0.0131 0.0209
Peroxisomes 0.0024 0 0.0004 0.0012 0 0 0.0003 0 0.0008 0.0004 0.004 0.0001 0.0025 0 0.001 0.0001 0 0
Punctate Nuclear 0.0086 0.0001 0.0014 0.0013 0.0001 0.0002 0.0019 0 0.0014 0.0026 0.001 0.0002 0.0022 0 0.0061 0.0008 0.0001 0.0003
Vacuole 0.005 0.0001 0.0011 0.0036 0.0101 0.0005 0.0018 0 0.0015 0.0003 0.0026 0.0024 0.0005 0 0.001 0.0004 0.0001 0.0103
Vacuole Periphery 0.0035 0 0.0004 0.003 0.0028 0.0002 0.0007 0 0.0009 0.0003 0.0044 0.0026 0.0005 0 0.0008 0.0001 0 0.0065

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 634.2403 704.1846 632.0372 522.7152 520.0519 691.7787 558.2135 523.8372 604.0553 549.8261
Translational Efficiency 1.5775 1.3595 1.3695 1.8426 2.0473 1.7834 1.7181 1.9345 1.7456 1.8804

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
122 1653 1054 222 2374 2303 1763 1542 2496 3956 2817 1764

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 2390.12 3325.26 5279.32 4901.38 3101.79 3536.03 4826.03 4548.51 3067.00 3447.96 4995.63 4592.92
Standard Deviation 507.58 778.14 959.46 755.34 628.09 591.79 829.14 877.70 641.36 683.88 907.08 871.16
Intensity Change Log 2 — 0.476384 1.143269 1.036105 — 0.189029 0.637736 0.552293 — 0.321172 0.879739 0.782990

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000953 0.000084 0.000068 0.000443 0.000047 0.000019 0.000056 0.000085 0.000091 0.000046 0.000060 0.000130
Bud Neck 0.004206 0.000272 0.000622 0.004823 0.000297 0.000197 0.000438 0.000514 0.000488 0.000229 0.000507 0.001056
Bud Site 0.011172 0.000595 0.000388 0.001550 0.000293 0.000289 0.000369 0.000681 0.000824 0.000417 0.000376 0.000790
Cell Periphery 0.001927 0.000175 0.000438 0.001988 0.000084 0.000055 0.000218 0.000260 0.000175 0.000105 0.000300 0.000477
Cytoplasm 0.580608* 0.626329* 0.445110* 0.381029* 0.613152* 0.644583* 0.488429* 0.543854* 0.611561* 0.636956* 0.472221* 0.523363*
Cytoplasmic Foci 0.018960 0.001935 0.006278 0.027337 0.001693 0.000771 0.003553 0.003880 0.002537 0.001258 0.004572 0.006832
Eisosomes 0.000007 0.000001 0.000001 0.000010 0.000000 0.000000 0.000001 0.000001 0.000001 0.000000 0.000001 0.000002
Endoplasmic Reticulum 0.000346 0.000027 0.000015 0.000101 0.000034 0.000024 0.000013 0.000015 0.000049 0.000025 0.000014 0.000026
Endosome 0.000312 0.000015 0.000010 0.000182 0.000020 0.000038 0.000013 0.000024 0.000035 0.000029 0.000012 0.000044
Golgi 0.003425 0.000194 0.000120 0.000677 0.000270 0.000072 0.000145 0.000103 0.000424 0.000123 0.000135 0.000176
Lipid Particles 0.001341 0.000075 0.000147 0.002404 0.000079 0.000031 0.000101 0.000142 0.000141 0.000049 0.000118 0.000426
Mitochondria 0.000922 0.000142 0.000220 0.000264 0.000136 0.000091 0.000154 0.000165 0.000174 0.000112 0.000179 0.000177
Mitotic Spindle 0.000624 0.000377 0.000135 0.000146 0.000280 0.000591 0.000378 0.000332 0.000297 0.000502 0.000287 0.000308
None 0.005201 0.023643 0.011257 0.005253 0.022232 0.025612 0.017800 0.015621 0.021400 0.024789 0.015352 0.014317
Nuclear Periphery 0.000169 0.000052 0.000209 0.000665 0.000065 0.000047 0.000124 0.000117 0.000070 0.000049 0.000156 0.000186
Nuclear Periphery Foci 0.002687 0.000399 0.000227 0.002132 0.000356 0.000137 0.000207 0.000314 0.000470 0.000247 0.000214 0.000542
Nucleolus 0.000260 0.000059 0.000046 0.000150 0.000064 0.000053 0.000047 0.000064 0.000074 0.000055 0.000046 0.000075
Nucleus 0.343064* 0.344177* 0.532140* 0.528974* 0.359608* 0.326045* 0.486001* 0.431470* 0.358799* 0.333622* 0.503264* 0.443741*
Peroxisomes 0.000043 0.000003 0.000003 0.000014 0.000004 0.000004 0.000003 0.000003 0.000006 0.000004 0.000003 0.000004
Vacuole 0.012727 0.001374 0.002532 0.041696 0.001140 0.001310 0.001913 0.002326 0.001707 0.001337 0.002144 0.007281
Vacuole Periphery 0.011046 0.000071 0.000035 0.000165 0.000144 0.000030 0.000039 0.000031 0.000677 0.000047 0.000037 0.000048

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 2.79 2.75 1.51 -7.90 -7.75 5.58 -3.92 -2.48 -4.38 -0.82 2.76 0.37 -2.10 -5.51 -3.20
Bud Neck 4.58 3.40 -3.02 -9.58 -8.19 2.82 -7.19 -5.24 -7.23 1.21 4.50 -5.69 -7.94 -10.72 -4.14
Bud Site 3.37 3.32 2.39 -7.53 -7.90 0.54 -4.64 -2.31 -2.41 -0.84 2.21 0.78 -1.37 -3.42 -2.31
Cell Periphery 4.64 3.06 -1.71 -9.00 -6.81 2.07 -8.94 -9.85 -9.79 -1.43 2.86 -9.29 -10.01 -11.99 -3.53
Cytoplasm -2.51 6.49 9.24 23.01 6.26 -4.72 24.21 13.43 17.91 -9.27 -4.55 29.62 17.14 23.10 -9.11
Cytoplasmic Foci 3.86 1.69 -4.57 -9.59 -7.55 5.35 -7.83 -7.92 -9.89 -1.50 4.26 -9.94 -10.46 -12.34 -4.37
Eisosomes 4.06 3.04 -3.22 -9.69 -8.43 1.86 -7.13 -6.47 -8.10 -0.31 3.22 -5.57 -8.60 -10.86 -5.23
Endoplasmic Reticulum 3.83 3.90 2.67 -4.94 -5.43 1.41 4.96 5.03 0.98 1.03 3.46 5.76 3.65 -0.53 -2.73
Endosome 2.78 2.73 0.49 -4.44 -4.35 -0.79 0.94 -0.23 0.58 -0.63 0.47 2.56 -1.19 -1.32 -2.69
Golgi 2.18 2.20 1.75 -5.61 -6.30 1.81 1.09 1.23 -2.33 0.17 2.23 1.97 1.55 -3.11 -1.65
Lipid Particles 2.91 2.09 -3.14 -5.96 -5.36 2.97 -4.55 -4.30 -6.13 -1.13 3.31 -4.11 -6.11 -7.53 -4.41
Mitochondria 3.29 2.68 2.77 -5.07 0.82 5.79 -4.29 -1.19 -2.90 0.49 4.43 -3.03 -0.36 -2.82 1.36
Mitotic Spindle 0.76 1.44 1.72 0.33 0.45 -0.81 -0.65 -0.70 0.37 -0.18 -0.68 -0.35 -0.58 0.21 -0.35
None -13.51 -9.13 0.66 13.96 10.48 -1.72 6.10 7.47 9.36 2.07 -2.26 7.88 8.30 11.85 1.55
Nuclear Periphery 4.47 -5.82 -10.76 -12.63 -8.25 2.38 -12.49 -8.38 -10.15 2.27 3.31 -15.61 -12.78 -14.58 -0.84
Nuclear Periphery Foci 2.94 2.99 -0.40 -5.87 -5.98 4.15 0.86 -1.36 -6.02 -2.79 3.58 2.16 -3.23 -7.02 -6.04
Nucleolus 3.22 3.23 1.12 -8.21 -9.46 0.69 -0.20 0.01 -0.61 0.19 1.36 0.81 -0.36 -1.81 -1.28
Nucleus 0.01 -9.22 -4.55 -7.68 5.60 4.85 -22.64 -12.35 -17.01 9.18 4.26 -28.40 -13.34 -18.82 12.64
Peroxisomes 3.63 3.48 1.45 -8.18 -7.51 -0.20 0.22 1.19 1.21 0.87 2.11 1.73 -0.17 -2.65 -2.21
Vacuole 4.39 2.97 -7.78 -10.14 -9.56 -2.99 -8.40 -9.15 -5.90 0.11 -0.07 -8.50 -10.47 -10.52 -7.41
Vacuole Periphery 1.19 1.19 1.18 -4.31 -7.81 1.29 1.18 1.20 -0.64 0.10 1.41 1.42 1.39 -0.62 -0.99
Standard name
Human Ortholog
Description Profilin; binds actin, phosphatidylinositol 4,5-bisphosphate, and polyproline regions; involved in cytoskeleton organization; required for normal timing of actin polymerization in response to thermal stress; protein abundance increases in response to DNA replication stress; highly conserved protein; human PFN1 (profilin 1) complements temperature sensitive pfy1 mutants, PFN1 mutations are a rare cause of ALS
Localization
Cell Percentages cytoplasm (99%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Pfy1

Pfy1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pfy1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available