Standard name
Human Ortholog
Description Phosphoribosylaminoimidazole carboxylase; catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway; red pigment accumulates in mutant cells deprived of adenine
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0.05 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0.06 0 0 0 0 – 0.1 0.08 0.1 0.09 0 0 0 0 0 0.05 0 0 0 0 0 0
Cytoplasm 1.0 0.99 0.98 0.97 0.99 0.94 0.86 – 0.81 0.83 0.78 0.76 1.0 0.99 0.98 0.99 0.98 0.98 0.99 0.97 0.93 0.83 0.92 0.95
Endoplasmic Reticulum 0 0 0 0 0 0 0.11 – 0.13 0.1 0.12 0.14 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 4 – 5 6 16 18 0 0 1 0 0 0 0 0 0 0 0 0
Bud 0 0 3 1 0 2 0 – 0 0 3 2 0 0 0 0 0 0 0 1 1 0 2 1
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 7 14 7 8 15 15 – 26 25 32 29 2 4 9 3 4 8 0 0 0 0 0 0
Cytoplasm 205 348 229 178 263 326 306 – 212 267 250 235 87 165 210 164 184 166 195 335 222 40 119 105
Endoplasmic Reticulum 0 3 2 1 2 11 40 – 33 32 38 44 1 3 3 7 5 9 0 1 5 0 1 0
Endosome 1 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 1 – 3 2 4 5 0 0 1 2 2 0 0 0 0 0 0 0
Mitochondria 0 2 0 0 1 0 0 – 1 4 13 4 0 0 1 0 0 1 0 0 0 0 1 0
Nucleus 0 0 0 0 0 0 2 – 1 0 0 0 0 0 0 0 0 0 0 0 1 3 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 3 1 1 1 0 – 5 8 3 6 0 1 0 1 0 1 0 2 3 2 1 0
Unique Cell Count 206 352 234 184 266 346 355 262 323 320 310 87 167 215 166 187 169 198 346 239 49 130 111
Labelled Cell Count 208 360 251 188 275 356 368 286 344 359 343 90 173 225 177 195 185 198 346 239 49 130 111


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 52.1 66.7 53.4 48.4 41.5 35.0 30.3 – 26.3 23.8 22.9 21.5 72.2 68.0 67.0 58.7 60.9 55.7 – 52.2 52.5
Std Deviation (1e-4) 10.1 13.1 11.9 10.7 10.2 7.4 6.8 – 6.3 5.5 5.4 4.7 16.6 23.0 19.1 13.2 12.8 17.5 – 16.5 16.9
Intensity Change (Log2) – – – -0.14 -0.36 -0.61 -0.82 – -1.02 -1.17 -1.22 -1.31 0.43 0.35 0.33 0.14 0.19 0.06 – -0.03 -0.03


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 3.5 3.7 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery -1.0 -1.6 -0.9 -1.0 – 1.6 0.8 1.7 1.4 0 -1.7 -0.9 -2.0 -1.9 -0.5
Cytoplasm -0.7 0.9 -2.1 -4.8 – -6.0 -5.7 -6.7 -7.2 1.4 0.7 -0.1 0.7 0.4 0.3
Endoplasmic Reticulum 0 0 1.9 4.8 – 5.1 4.4 5.0 5.5 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 – 0 0 3.1 0 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 70.2039 82.2245 77.0893 73.7538 70.7101 75.6757 51.4515 74.8677 67.1798 61.2992 50.9428 68.361 64.1869 93.0089 86.6778 71.3607 67.8582 78.0674
Actin 0.0001 0.0001 0.0001 0 0.0001 0.0001 0.0306 0.0008 0.0043 0.0009 0.0229 0.0004 0.0161 0.0001 0.0147 0.0017 0.011 0.0007
Bud 0.0001 0.0002 0.0001 0.0001 0.0001 0.0001 0.0016 0.0002 0.0005 0.0011 0.0046 0.0013 0.0012 0.0002 0.0003 0.002 0.0008 0.0014
Bud Neck 0.0003 0.0003 0.0006 0.0003 0.0012 0.0019 0.0006 0.0001 0.0003 0.0003 0.0009 0.0007 0.0004 0.0001 0.0004 0.0005 0.0003 0.0006
Bud Periphery 0.0001 0.0002 0.0001 0.0001 0 0.0002 0.0021 0.0002 0.0005 0.0004 0.0061 0.0043 0.0013 0 0.0003 0.0044 0.0011 0.0002
Bud Site 0 0.0001 0 0 0.0001 0 0.0038 0.001 0.0038 0.0021 0.0068 0.0006 0.0012 0 0.0019 0.003 0.0007 0.0001
Cell Periphery 0.0037 0.0013 0.0019 0.0027 0.0009 0.0023 0.001 0.0004 0.0004 0.0038 0.0017 0.0003 0.0006 0.0001 0.0001 0.0004 0.0001 0.0002
Cytoplasm 0.9338 0.9653 0.9615 0.9174 0.9214 0.9392 0.8458 0.983 0.9232 0.8869 0.7524 0.9296 0.8503 0.9938 0.9595 0.8339 0.8612 0.9788
Cytoplasmic Foci 0.0006 0.0004 0.0006 0.0005 0.0032 0.001 0.0117 0.0006 0.0068 0.0064 0.0126 0.0009 0.0105 0.0003 0.0053 0.0031 0.0076 0.0022
Eisosomes 0 0 0 0 0 0 0.0005 0 0.0001 0.0007 0.0003 0 0.0003 0 0.0001 0 0.0001 0
Endoplasmic Reticulum 0.0268 0.0199 0.0179 0.0302 0.0281 0.0369 0.0143 0.0024 0.0049 0.001 0.0132 0.0106 0.0093 0.0018 0.0008 0.0028 0.002 0.0011
Endosome 0.0009 0.0005 0.001 0.0013 0.0034 0.0019 0.0147 0.0017 0.0098 0.0025 0.0725 0.0126 0.0239 0.0003 0.0017 0.0586 0.0391 0.0015
Golgi 0.0002 0.0002 0.0001 0.0001 0.0007 0.0003 0.0048 0.0002 0.0033 0.0003 0.0081 0.0008 0.0033 0 0.0012 0.0113 0.0209 0.0001
Lipid Particles 0.0009 0.0007 0.0004 0.0003 0.0054 0.0004 0.0059 0.0004 0.0041 0.0064 0.0057 0.0002 0.008 0 0.0011 0.0054 0.0132 0.0001
Mitochondria 0.0002 0.0007 0.0005 0 0.0012 0.0012 0.0205 0.0022 0.0086 0.0065 0.0235 0.0016 0.0195 0 0.0021 0.0186 0.0192 0.0002
None 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0049 0.0003 0.0021 0.0077 0.0106 0.0002 0.0029 0.0001 0.001 0.0004 0.0007 0.0004
Nuclear Periphery 0.0054 0.0016 0.0019 0.0028 0.0035 0.0029 0.0084 0.0013 0.0035 0.0056 0.012 0.0026 0.0134 0.0007 0.0016 0.011 0.0078 0.0026
Nucleolus 0 0 0 0.0001 0.0001 0 0.0021 0.0001 0.001 0.0261 0.0028 0.0002 0.0042 0 0.0001 0.001 0.0003 0
Nucleus 0.0019 0.0009 0.001 0.0012 0.0013 0.0014 0.0053 0.0025 0.0023 0.0092 0.0097 0.0022 0.0098 0.0006 0.0012 0.0034 0.0015 0.0033
Peroxisomes 0.0016 0 0 0 0.0086 0 0.0014 0 0.0007 0.0005 0.0013 0 0.0011 0 0.0032 0.0009 0.0049 0
Punctate Nuclear 0 0 0 0 0.0001 0 0.0014 0.0002 0.0088 0.001 0.0012 0.0001 0.0027 0 0.0016 0.0005 0.0018 0.0003
Vacuole 0.0215 0.007 0.0112 0.0404 0.0187 0.0088 0.0137 0.0019 0.0079 0.0175 0.0206 0.0247 0.0123 0.0017 0.0014 0.0244 0.0029 0.0045
Vacuole Periphery 0.0016 0.0004 0.0007 0.0022 0.0019 0.0011 0.0049 0.0003 0.0034 0.013 0.0106 0.006 0.0078 0.0003 0.0005 0.0128 0.0028 0.0018

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 29.2569 27.0161 25.1656 27.7001 22.9265 44.3431 30.9484 37.069 43.5113 35.6214
Translational Efficiency 1.9222 1.4826 1.5288 1.6479 1.6507 1.5336 1.7367 1.4674 1.5059 1.638

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Phosphoribosylaminoimidazole carboxylase; catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway; red pigment accumulates in mutant cells deprived of adenine
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-2

Ade2

Ade2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ade2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available