Standard name | |
---|---|
Human Ortholog | |
Description | Subunit of the membrane-associated retromer complex; essential for endosome-to-Golgi retrograde protein transport; peripheral membrane protein that assembles onto the membrane with Vps5p to promote vesicle formation; required for recruiting the retromer complex to the endosome membranes |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 3 | 2 | 1 | 0 | 1 | 2 | 10 | – | 6 | 11 | 17 | 13 | 11 | 4 | 3 | 3 | 5 | 0 | 1 | 5 | 1 | 4 | 7 |
Bud | 0 | 1 | 4 | 3 | 0 | 10 | 11 | 36 | – | 29 | 37 | 38 | 1 | 1 | 1 | 2 | 1 | 0 | 0 | 0 | 3 | 1 | 0 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 2 | 12 | 20 | 15 | 9 | 14 | 18 | 41 | – | 19 | 18 | 28 | 21 | 21 | 17 | 33 | 28 | 18 | 0 | 1 | 1 | 0 | 2 | 3 |
Endoplasmic Reticulum | 0 | 10 | 4 | 1 | 6 | 8 | 13 | 14 | – | 12 | 10 | 17 | 7 | 8 | 5 | 12 | 19 | 4 | 0 | 2 | 3 | 1 | 2 | 6 |
Endosome | 40 | 75 | 345 | 235 | 306 | 346 | 359 | 525 | – | 189 | 134 | 262 | 305 | 312 | 343 | 171 | 254 | 102 | 77 | 185 | 367 | 416 | 688 | 772 |
Golgi | 2 | 15 | 5 | 7 | 11 | 4 | 13 | 9 | – | 12 | 5 | 14 | 11 | 14 | 32 | 3 | 12 | 15 | 4 | 8 | 17 | 14 | 59 | 53 |
Mitochondria | 12 | 29 | 43 | 85 | 60 | 114 | 143 | 293 | – | 321 | 274 | 304 | 2 | 2 | 0 | 25 | 45 | 14 | 2 | 2 | 5 | 7 | 10 | 9 |
Nucleus | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | 0 | 3 | 1 | 4 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 2 | 4 | 4 | 3 | 7 | 4 | 3 | – | 6 | 1 | 7 | 9 | 6 | 4 | 0 | 7 | 0 | 0 | 0 | 1 | 0 | 2 | 2 |
Nucleolus | 17 | 23 | 58 | 40 | 51 | 46 | 50 | 74 | – | 52 | 24 | 36 | 28 | 28 | 43 | 11 | 14 | 17 | 0 | 1 | 0 | 1 | 1 | 3 |
Peroxisomes | 0 | 0 | 13 | 15 | 5 | 52 | 22 | 43 | – | 16 | 19 | 12 | 3 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 8 | 3 | 7 | 5 |
SpindlePole | 6 | 6 | 41 | 27 | 33 | 47 | 40 | 81 | – | 25 | 23 | 38 | 64 | 65 | 53 | 33 | 33 | 8 | 6 | 2 | 14 | 7 | 15 | 13 |
Vac/Vac Membrane | 26 | 104 | 50 | 31 | 61 | 45 | 37 | 67 | – | 38 | 22 | 27 | 182 | 183 | 288 | 108 | 172 | 82 | 4 | 17 | 1 | 16 | 10 | 20 |
Unique Cell Count | 72 | 202 | 413 | 328 | 386 | 470 | 546 | 878 | 559 | 452 | 609 | 462 | 466 | 548 | 268 | 380 | 176 | 99 | 226 | 435 | 479 | 811 | 903 | |
Labelled Cell Count | 107 | 281 | 591 | 465 | 546 | 694 | 712 | 1196 | 726 | 579 | 802 | 646 | 653 | 794 | 402 | 592 | 267 | 99 | 226 | 435 | 479 | 811 | 903 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.2 | 9.2 | 7.8 | 7.8 | 7.9 | 7.1 | 7.1 | 7.0 | – | 6.5 | 6.7 | 6.8 | 9.2 | 9.2 | 10.4 | 7.6 | 8.9 | 10.4 | 9.6 | 10.0 | 10.5 |
Std Deviation (1e-4) | 1.2 | 1.5 | 1.6 | 1.5 | 1.4 | 1.2 | 1.2 | 1.3 | – | 1.3 | 1.4 | 1.4 | 1.1 | 1.1 | 1.4 | 1.4 | 1.9 | 2.3 | 1.3 | 1.5 | 1.8 |
Intensity Change (Log2) | – | – | – | 0.01 | 0.02 | -0.14 | -0.14 | -0.14 | – | -0.25 | -0.22 | -0.2 | 0.24 | 0.24 | 0.42 | -0.03 | 0.2 | 0.42 | 0.3 | 0.37 | 0.43 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | – | 0 | 2.4 | 2.7 | 2.7 | 2.3 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 1.4 | 1.3 | 3.0 | – | 3.6 | 5.0 | 4.2 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -0.2 | -1.9 | -1.4 | -1.2 | -0.1 | – | -1.1 | -0.6 | -0.2 | -0.2 | -0.2 | -1.4 | 3.6 | 1.5 | 2.4 |
Endoplasmic Reticulum | 0 | 0 | 0.9 | 1.6 | 0.9 | – | 1.4 | 1.4 | 2.0 | 0.7 | 1.0 | 0 | 3.0 | 3.4 | 0 |
Endosome | -3.9 | -1.5 | -3.6 | -6.2 | -8.5 | – | -15.4 | -15.9 | -12.9 | -5.9 | -5.6 | -7.1 | -5.9 | -5.5 | -6.6 |
Golgi | 1.0 | 1.7 | 0 | 1.3 | 0 | – | 1.1 | 0 | 1.3 | 1.3 | 1.8 | 3.7 | 0 | 1.9 | 4.5 |
Mitochondria | 5.5 | 2.2 | 5.4 | 6.1 | 8.8 | – | 15.0 | 15.3 | 13.1 | -6.7 | -6.7 | -7.7 | -0.5 | 0.6 | -0.9 |
Nucleus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0.7 | 0 | 0 | – | 0 | 0 | 0 | 1.2 | 0 | 0 | 0 | 1.1 | 0 |
Nucleolus | -0.7 | -0.3 | -2.0 | -2.4 | -3.1 | – | -2.3 | -4.4 | -4.4 | -4.0 | -4.0 | -3.1 | -4.2 | -5.1 | -1.5 |
Peroxisomes | 1.0 | -1.8 | 4.5 | 0.7 | 1.4 | – | -0.3 | 0.8 | -1.2 | -2.8 | -3.1 | -4.2 | -2.9 | -3.5 | 0 |
SpindlePole | -0.8 | -0.7 | 0 | -1.4 | -0.4 | – | -3.3 | -2.7 | -2.2 | 1.8 | 1.8 | -0.1 | 1.0 | -0.6 | -2.2 |
Vacuole | -1.2 | 1.5 | -1.2 | -2.8 | -2.6 | – | -2.9 | -3.8 | -4.6 | 9.1 | 9.1 | 13.0 | 8.5 | 10.4 | 9.2 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 5.6933 | 6.9236 | 6.7606 | 6.0472 | 7.3385 | 6.0801 | 4.4976 | 4.3391 | 4.762 | 3.9591 | 4.6273 | 4.914 | 3.5401 | 4.9648 | 4.9043 | 9.7283 | 3.9447 | 4.6425 |
Actin | 0.0004 | 0.0026 | 0.0006 | 0.0001 | 0 | 0.0001 | 0.0141 | 0.0001 | 0.0073 | 0.0392 | 0 | 0.0024 | 0.0001 | 0.0001 | 0.0137 | 0 | 0 | 0.0131 |
Bud | 0.0001 | 0.0081 | 0.0076 | 0.0001 | 0 | 0.0002 | 0.0002 | 0.0003 | 0.0029 | 0.0003 | 0.0009 | 0.0001 | 0 | 0.0001 | 0.0007 | 0 | 0 | 0.0001 |
Bud Neck | 0.0003 | 0.0005 | 0.0005 | 0.0001 | 0.0003 | 0.0003 | 0.0003 | 0.0004 | 0.0009 | 0.0004 | 0.0003 | 0.0005 | 0.0002 | 0.0003 | 0.0005 | 0.0001 | 0.0001 | 0.0005 |
Bud Periphery | 0.0001 | 0.0025 | 0.0016 | 0 | 0 | 0.0001 | 0.0002 | 0.0001 | 0.0032 | 0.0004 | 0.0004 | 0 | 0 | 0 | 0.0005 | 0 | 0 | 0.0001 |
Bud Site | 0.0004 | 0.0141 | 0.0039 | 0.0001 | 0.0001 | 0.0001 | 0.001 | 0.0033 | 0.0184 | 0.0013 | 0.0051 | 0.0003 | 0.0001 | 0.0005 | 0.0016 | 0 | 0.0001 | 0.0006 |
Cell Periphery | 0 | 0.0002 | 0.0004 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0 | 0 | 0.0001 | 0.0002 | 0 | 0 | 0.0001 |
Cytoplasm | 0.004 | 0.0153 | 0.0111 | 0.0014 | 0.0014 | 0.0032 | 0.0351 | 0.0923 | 0.0562 | 0.0111 | 0.0331 | 0.0041 | 0.0015 | 0.0162 | 0.0072 | 0.0035 | 0.0013 | 0.009 |
Cytoplasmic Foci | 0.1446 | 0.182 | 0.1876 | 0.1144 | 0.1446 | 0.1772 | 0.1241 | 0.1215 | 0.1723 | 0.129 | 0.1297 | 0.1492 | 0.2093 | 0.1864 | 0.1638 | 0.1172 | 0.1476 | 0.1568 |
Eisosomes | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0.0007 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0005 | 0.0006 | 0.0014 | 0.0002 | 0.0002 | 0.0004 | 0.0234 | 0.0019 | 0.0016 | 0.0042 | 0.0024 | 0.0005 | 0.0003 | 0.0034 | 0.001 | 0.0002 | 0.0002 | 0.0011 |
Endosome | 0.6907 | 0.6281 | 0.6292 | 0.7563 | 0.7348 | 0.6188 | 0.4828 | 0.5986 | 0.4915 | 0.6602 | 0.677 | 0.5907 | 0.5963 | 0.6165 | 0.6021 | 0.6915 | 0.7122 | 0.5767 |
Golgi | 0.0693 | 0.0791 | 0.0889 | 0.1051 | 0.0831 | 0.0925 | 0.0517 | 0.0348 | 0.1175 | 0.0976 | 0.0706 | 0.155 | 0.1042 | 0.064 | 0.13 | 0.0587 | 0.0975 | 0.1517 |
Lipid Particles | 0.0367 | 0.0236 | 0.0252 | 0.0091 | 0.0176 | 0.0645 | 0.0599 | 0.023 | 0.0491 | 0.0078 | 0.0141 | 0.0655 | 0.0673 | 0.0432 | 0.0225 | 0.0041 | 0.0211 | 0.0488 |
Mitochondria | 0.0038 | 0.0011 | 0.0011 | 0.0007 | 0.0009 | 0.0014 | 0.0024 | 0.0008 | 0.0035 | 0.0076 | 0.0024 | 0.0028 | 0.0016 | 0.0015 | 0.0069 | 0.0014 | 0.0005 | 0.0022 |
None | 0.0002 | 0.0003 | 0.0008 | 0 | 0 | 0.0004 | 0.0796 | 0.0121 | 0.0143 | 0.0033 | 0.0005 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0 | 0 | 0.0014 |
Nuclear Periphery | 0.0012 | 0.0005 | 0.0035 | 0.0001 | 0.0001 | 0.0002 | 0.0624 | 0.0021 | 0.0019 | 0.0029 | 0.0009 | 0.0004 | 0.0002 | 0.0021 | 0.0005 | 0.0002 | 0.0002 | 0.0014 |
Nucleolus | 0.0002 | 0.0005 | 0.0004 | 0 | 0.0002 | 0.0003 | 0.0003 | 0.0005 | 0.0009 | 0.0002 | 0.0004 | 0.0003 | 0.0001 | 0.0003 | 0.0076 | 0.1009 | 0.0001 | 0.0003 |
Nucleus | 0.0005 | 0.0013 | 0.0008 | 0 | 0.0001 | 0.0002 | 0.0032 | 0.0029 | 0.0091 | 0.0006 | 0.001 | 0.0001 | 0 | 0.0005 | 0.0019 | 0.0089 | 0.0001 | 0.0007 |
Peroxisomes | 0.023 | 0.0177 | 0.0221 | 0.0044 | 0.0057 | 0.0274 | 0.0082 | 0.0067 | 0.0123 | 0.0028 | 0.0123 | 0.0069 | 0.01 | 0.0062 | 0.0162 | 0.004 | 0.0042 | 0.0101 |
Punctate Nuclear | 0.0014 | 0.0041 | 0.0008 | 0.0001 | 0.0002 | 0.001 | 0.0067 | 0.0007 | 0.0021 | 0.0008 | 0.0003 | 0.0008 | 0.002 | 0.0005 | 0.0086 | 0.0001 | 0.0002 | 0.0025 |
Vacuole | 0.0187 | 0.015 | 0.01 | 0.0047 | 0.0086 | 0.0085 | 0.0371 | 0.0875 | 0.0294 | 0.0248 | 0.042 | 0.0156 | 0.0044 | 0.0508 | 0.0114 | 0.0057 | 0.0116 | 0.0183 |
Vacuole Periphery | 0.0039 | 0.003 | 0.0025 | 0.003 | 0.0021 | 0.0033 | 0.007 | 0.0104 | 0.0051 | 0.0047 | 0.0067 | 0.0047 | 0.0025 | 0.007 | 0.0029 | 0.0032 | 0.0028 | 0.0045 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 32.3159 | 15.4866 | 17.6796 | 33.373 | 25.4849 | 42.62 | 26.4989 | 26.2178 | 32.3571 | 33.901 |
Translational Efficiency | 1.3255 | 1.3991 | 1.3318 | 0.975 | 1.1526 | 1.2717 | 1.1196 | 0.9915 | 1.1749 | 1.0916 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
95 | 108 | 1314 | 902 | 246 | 90 | 3084 | 7 | 341 | 198 | 4398 | 909 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1229.60 | 1061.39 | 873.89 | 1127.51 | 849.30 | 1021.65 | 1060.08 | 468.34 | 955.25 | 1043.33 | 1004.45 | 1122.43 |
Standard Deviation | 203.20 | 147.90 | 212.46 | 195.28 | 122.83 | 114.08 | 155.28 | 253.05 | 226.83 | 135.05 | 194.05 | 204.09 |
Intensity Change Log 2 | — | -0.212234 | -0.492665 | -0.125049 | — | 0.266555 | 0.319827 | -0.858718 | — | 0.002870 | -0.104255 | -0.381495 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.008420 | 0.000183 | 0.000313 | 0.002510 | 0.000076 | 0.000083 | 0.000082 | 0.000055 | 0.002400 | 0.000138 | 0.000151 | 0.002491 |
Bud Neck | 0.005692 | 0.000908 | 0.003349 | 0.001325 | 0.001948 | 0.004386 | 0.000500 | 0.000045 | 0.002991 | 0.002489 | 0.001351 | 0.001315 |
Bud Site | 0.015655 | 0.003493 | 0.013930 | 0.012499 | 0.003636 | 0.007845 | 0.004203 | 0.000270 | 0.006984 | 0.005471 | 0.007109 | 0.012405 |
Cell Periphery | 0.006127 | 0.000053 | 0.000098 | 0.000103 | 0.000098 | 0.000303 | 0.000077 | 0.000075 | 0.001778 | 0.000167 | 0.000083 | 0.000103 |
Cytoplasm | 0.027897 | 0.001283 | 0.012929 | 0.005722 | 0.002999 | 0.002319 | 0.002389 | 0.001393 | 0.009935 | 0.001754 | 0.005538 | 0.005689 |
Cytoplasmic Foci | 0.144043 | 0.109788 | 0.145959 | 0.149586 | 0.080828 | 0.090962 | 0.058957 | 0.019195 | 0.098439 | 0.101231 | 0.084951 | 0.148582 |
Eisosomes | 0.000246 | 0.000016 | 0.000020 | 0.000023 | 0.000015 | 0.000020 | 0.000005 | 0.000255 | 0.000079 | 0.000018 | 0.000009 | 0.000025 |
Endoplasmic Reticulum | 0.016169 | 0.000135 | 0.000527 | 0.000340 | 0.000120 | 0.000106 | 0.000054 | 0.000001 | 0.004591 | 0.000122 | 0.000195 | 0.000338 |
Endosome | 0.435509* | 0.696934* | 0.539654* | 0.406375* | 0.759376* | 0.704202* | 0.680706* | 0.109377 | 0.669149* | 0.700238* | 0.638564* | 0.404088* |
Golgi | 0.047771 | 0.105856 | 0.101875 | 0.284025* | 0.086603 | 0.073548 | 0.125808* | 0.006246 | 0.075784 | 0.091171 | 0.118657* | 0.281886* |
Lipid Particles | 0.011863 | 0.003009 | 0.008899 | 0.006989 | 0.005223 | 0.025331 | 0.001035 | 0.000019 | 0.007073 | 0.013155 | 0.003384 | 0.006935 |
Mitochondria | 0.002398 | 0.006309 | 0.003174 | 0.003828 | 0.002924 | 0.001590 | 0.007235 | 0.000058 | 0.002777 | 0.004164 | 0.006022 | 0.003799 |
Mitotic Spindle | 0.017225 | 0.000988 | 0.012006 | 0.006283 | 0.000415 | 0.001314 | 0.004947 | 0.000867 | 0.005098 | 0.001136 | 0.007056 | 0.006241 |
None | 0.000239 | 0.000008 | 0.001037 | 0.000022 | 0.000011 | 0.000015 | 0.000020 | 0.856568* | 0.000075 | 0.000011 | 0.000324 | 0.006618 |
Nuclear Periphery | 0.000385 | 0.000082 | 0.000382 | 0.000205 | 0.000089 | 0.000363 | 0.000155 | 0.000005 | 0.000172 | 0.000210 | 0.000223 | 0.000204 |
Nuclear Periphery Foci | 0.002114 | 0.000980 | 0.015264 | 0.001831 | 0.005287 | 0.002565 | 0.001097 | 0.000005 | 0.004403 | 0.001701 | 0.005330 | 0.001817 |
Nucleolus | 0.000643 | 0.000021 | 0.003819 | 0.000102 | 0.000549 | 0.000640 | 0.000055 | 0.000004 | 0.000575 | 0.000302 | 0.001179 | 0.000102 |
Nucleus | 0.019176 | 0.000024 | 0.003981 | 0.000135 | 0.000099 | 0.000154 | 0.000032 | 0.000003 | 0.005413 | 0.000083 | 0.001212 | 0.000134 |
Peroxisomes | 0.023343 | 0.005139 | 0.012958 | 0.008832 | 0.011725 | 0.018563 | 0.003185 | 0.001994 | 0.014962 | 0.011241 | 0.006105 | 0.008779 |
Vacuole | 0.201146* | 0.053940 | 0.098240 | 0.086751 | 0.027721 | 0.037675 | 0.080168 | 0.002923 | 0.076036 | 0.046547 | 0.085567 | 0.086105 |
Vacuole Periphery | 0.013938 | 0.010851 | 0.021588 | 0.022514 | 0.010261 | 0.028015 | 0.029291 | 0.000642 | 0.011285 | 0.018652 | 0.026990 | 0.022345 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 1.15 | 1.13 | 0.82 | -3.78 | -3.58 | NaN | NaN | NaN | NaN | NaN | 1.13 | 1.12 | -0.06 | -3.88 | -3.87 |
Bud Neck | 4.42 | 2.07 | 4.09 | -1.18 | 4.38 | NaN | NaN | NaN | NaN | NaN | 0.51 | 3.74 | 3.53 | 1.22 | 0.10 |
Bud Site | 3.65 | 0.47 | 0.85 | -4.41 | 0.65 | NaN | NaN | NaN | NaN | NaN | 0.57 | -0.24 | -3.00 | -2.48 | -3.23 |
Cell Periphery | 1.30 | 1.29 | 1.29 | -2.47 | -0.29 | NaN | NaN | NaN | NaN | NaN | 1.23 | 1.30 | 1.28 | 0.67 | -0.67 |
Cytoplasm | 2.57 | 1.35 | 1.97 | -3.54 | 2.63 | NaN | NaN | NaN | NaN | NaN | 2.57 | 1.31 | 0.76 | -3.10 | -0.87 |
Cytoplasmic Foci | 1.69 | 0.03 | -0.17 | -2.69 | -0.52 | NaN | NaN | NaN | NaN | NaN | -0.21 | 2.00 | -5.72 | -4.42 | -11.62 |
Eisosomes | 2.64 | 2.60 | 2.57 | -0.83 | -1.00 | NaN | NaN | NaN | NaN | NaN | 2.42 | 2.80 | 2.18 | -1.18 | -5.79 |
Endoplasmic Reticulum | 1.60 | 1.56 | 1.58 | -1.98 | 1.82 | NaN | NaN | NaN | NaN | NaN | 1.59 | 1.56 | 1.51 | -2.36 | -1.62 |
Endosome | -7.03 | -3.31 | 0.87 | 12.63 | 12.36 | NaN | NaN | NaN | NaN | NaN | -1.40 | 1.96 | 15.39 | 16.27 | 26.59* |
Golgi | -3.74 | -4.85 | -17.55 | -12.44 | -20.28* | NaN | NaN | NaN | NaN | NaN | -1.50 | -6.14 | -19.43 | -16.49 | -19.06 |
Lipid Particles | 1.63 | 0.53 | 0.88 | -2.51 | 1.06 | NaN | NaN | NaN | NaN | NaN | -0.98 | 1.49 | 0.04 | 1.06 | -2.67 |
Mitochondria | -0.78 | -0.64 | -1.00 | 0.50 | -0.69 | NaN | NaN | NaN | NaN | NaN | -0.50 | -3.77 | -0.96 | 0.13 | 2.43 |
Mitotic Spindle | 2.08 | 0.61 | 1.31 | -3.53 | 2.44 | NaN | NaN | NaN | NaN | NaN | 1.77 | -0.94 | -0.64 | -3.52 | 0.27 |
None | 1.18 | -1.36 | 1.10 | -2.88 | 1.82 | NaN | NaN | NaN | NaN | NaN | 1.16 | -1.43 | -2.44 | -2.46 | -2.34 |
Nuclear Periphery | 2.32 | -0.02 | 1.37 | -2.43 | 3.21 | NaN | NaN | NaN | NaN | NaN | -0.27 | -1.08 | -0.54 | 0.04 | 0.54 |
Nuclear Periphery Foci | 1.51 | -6.37 | 0.46 | -1.48 | 6.76 | NaN | NaN | NaN | NaN | NaN | 0.91 | -0.35 | 0.89 | -0.15 | 4.92 |
Nucleolus | 2.10 | -3.48 | 1.81 | -1.99 | 4.26 | NaN | NaN | NaN | NaN | NaN | 0.69 | -1.41 | 1.30 | 1.12 | 4.07 |
Nucleus | 1.95 | 1.56 | 1.91 | -1.39 | 3.42 | NaN | NaN | NaN | NaN | NaN | 1.93 | 1.53 | 1.81 | -1.18 | 1.93 |
Peroxisomes | 3.07 | 1.79 | 2.49 | -1.96 | 2.62 | NaN | NaN | NaN | NaN | NaN | 1.14 | 3.69 | 2.39 | 0.98 | -2.20 |
Vacuole | 5.08 | 3.75 | 4.15 | -2.85 | 1.93 | NaN | NaN | NaN | NaN | NaN | 2.57 | -1.12 | -1.13 | -4.91 | -0.22 |
Vacuole Periphery | 0.88 | -2.50 | -2.65 | -4.64 | -0.45 | NaN | NaN | NaN | NaN | NaN | -1.65 | -8.82 | -4.87 | -0.80 | 2.55 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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