Standard name
Human Ortholog
Description Thiamine pyrophosphokinase; phosphorylates thiamine to produce the coenzyme thiamine pyrophosphate (thiamine diphosphate)
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Cytoplasm 0.94 0.97 0.99 0.97 0.97 0.92 0.75 0.83 0.73 0.73 0.67 0.77 0.98 0.99 0.99 0.94 0.97 0.94 0.94 0.87 0.83 0.94 0.92 0.91
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.05 0 0 0 0 0.1 0.27 0.16 0.35 0.26 0.39 0.21 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0.09 0.08 0 0.1 0 0.09 0 0 0 0.11 0.07 0 0 0.07 0.09 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1
Bud 0 0 0 0 1 0 4 5 3 10 8 4 0 0 0 0 0 0 0 0 0 2 2 7
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 4 2 3 4 5 5 1 0 3 2 5 0 2 5 9 9 0 0 0 0 0 0
Cytoplasm 153 182 186 232 311 297 337 331 216 220 225 259 333 465 552 176 242 182 157 171 166 269 367 433
Endoplasmic Reticulum 2 4 0 0 1 2 1 1 3 0 0 1 4 0 2 6 8 4 0 0 2 0 3 4
Endosome 0 0 0 0 0 0 1 0 0 0 0 0 1 0 2 2 0 0 3 1 1 1 2 2
Golgi 1 0 0 1 0 0 0 0 0 0 0 0 0 1 0 1 0 1 0 1 0 0 1 1
Mitochondria 8 1 0 7 3 32 122 63 102 80 131 72 0 0 0 1 2 1 1 2 6 0 4 4
Nucleus 1 1 2 2 2 3 9 10 12 10 9 14 2 3 1 0 2 3 0 2 1 0 0 1
Nuclear Periphery 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1
Nucleolus 0 0 0 1 0 0 1 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 2 1 1
Vac/Vac Membrane 0 0 0 3 6 6 42 32 6 30 14 29 0 10 2 20 18 7 1 13 18 1 3 3
Unique Cell Count 162 187 187 239 321 323 447 399 294 303 335 337 340 471 555 188 250 193 168 196 200 286 400 474
Labelled Cell Count 165 188 192 249 328 344 522 449 343 350 390 382 345 479 561 212 281 208 168 196 200 286 400 474


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.2 7.4 6.3 6.4 6.4 5.4 5.1 5.6 4.9 5.1 4.8 5.3 7.4 7.3 7.0 9.8 9.2 8.8 7.0 7.1 7.6
Std Deviation (1e-4) 0.7 1.1 0.8 1.3 1.3 1.0 1.3 1.0 1.1 1.3 1.0 1.1 0.9 0.9 0.9 2.0 1.5 1.7 1.1 1.1 1.6
Intensity Change (Log2) – – – 0.03 0.02 -0.21 -0.31 -0.17 -0.36 -0.29 -0.4 -0.25 0.23 0.21 0.15 0.64 0.55 0.49 0.15 0.17 0.27


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0.9 1.4
Cytoplasm -1.8 -1.9 -3.7 -7.3 -5.8 -7.5 -7.7 -8.6 -6.9 -1.4 -0.8 0 -3.1 -1.9 -2.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 4.4 7.9 5.8 9.1 7.7 0 6.8 0 0 0 0 0 0
Nucleus 0 0 0 0 0 1.9 0 0 2.0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 4.3 4.0 0 4.4 2.8 4.1 0 0 0 4.6 3.7 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.2917 1.9996 1.5895 0.9 1.0757 1.4846 2.0816 2.9341 2.4423 2.4259 1.966 2.6332 2.0892 2.9938 2.4763 2.5668 2.302 2.4842
Actin 0.0108 0.0019 0.0019 0.003 0.0019 0.0021 0.0235 0.0005 0.0015 0.0011 0.0441 0.0074 0.0022 0.0005 0.0008 0.0004 0.007 0.0009
Bud 0.0012 0.0061 0.0018 0.0011 0.0006 0.0023 0.0076 0.0015 0.0051 0.0101 0.0005 0.0023 0.0085 0.0093 0.0088 0.0002 0.0036 0.0025
Bud Neck 0.0068 0.0003 0.0017 0.0006 0.0008 0.0016 0.0034 0.0005 0.0006 0.0012 0.0018 0.0056 0.0004 0.0003 0.0007 0.0005 0.004 0.0013
Bud Periphery 0.0018 0.0015 0.0013 0.0006 0.0003 0.0005 0.0049 0.0004 0.0012 0.0035 0.0006 0.0012 0.0029 0.0028 0.0033 0.0001 0.0013 0.001
Bud Site 0.004 0.0059 0.0073 0.0008 0.0002 0.0002 0.0034 0.0096 0.0062 0.0008 0.001 0.0007 0.0055 0.0062 0.0047 0.0002 0.001 0.0004
Cell Periphery 0.0002 0.0002 0.0001 0 0 0 0.0002 0.0002 0.0001 0.0002 0.0001 0.0001 0.0002 0.0006 0.0002 0 0.0001 0.0001
Cytoplasm 0.6583 0.8163 0.719 0.7565 0.8117 0.7991 0.6375 0.8037 0.7681 0.7264 0.6806 0.7945 0.844 0.8657 0.8443 0.8536 0.8311 0.8784
Cytoplasmic Foci 0.0171 0.0072 0.0185 0.019 0.0168 0.0155 0.0321 0.0141 0.0128 0.014 0.0337 0.0227 0.0119 0.0092 0.0079 0.0092 0.0192 0.0116
Eisosomes 0.0002 0 0 0 0 0 0.0003 0 0 0 0.0002 0 0 0 0 0 0.0001 0
Endoplasmic Reticulum 0.0054 0.0027 0.0023 0.0113 0.0029 0.0022 0.0054 0.0027 0.0019 0.0021 0.0046 0.0014 0.0028 0.0028 0.0028 0.0028 0.004 0.0016
Endosome 0.0237 0.0037 0.0036 0.0155 0.0055 0.0063 0.0272 0.0054 0.0041 0.0111 0.0394 0.0071 0.0046 0.0039 0.0037 0.0049 0.0107 0.0052
Golgi 0.0023 0.0003 0.0004 0.001 0.0003 0.0002 0.0063 0.0004 0.0004 0.0007 0.0049 0.0011 0.0004 0.0002 0.0002 0.0002 0.0022 0.0002
Lipid Particles 0.002 0.0001 0.0002 0.0006 0.0003 0.0002 0.0071 0.0001 0.0002 0.0007 0.0035 0.0018 0.001 0.0001 0.0001 0.0001 0.0007 0.0001
Mitochondria 0.0012 0.0005 0.0004 0.0008 0.0005 0.0004 0.0047 0.0005 0.0006 0.001 0.0032 0.0011 0.0007 0.0005 0.0006 0.0005 0.0005 0.0004
None 0.2266 0.1361 0.2175 0.1602 0.1192 0.1403 0.187 0.1425 0.1756 0.1677 0.1313 0.1206 0.0953 0.0796 0.0875 0.0999 0.0885 0.0757
Nuclear Periphery 0.0056 0.0022 0.002 0.01 0.0039 0.002 0.0089 0.0018 0.002 0.0164 0.0063 0.0036 0.0018 0.0023 0.0026 0.0026 0.0027 0.0016
Nucleolus 0.0015 0.0002 0.0002 0.0002 0.0001 0.0004 0.001 0.0002 0.0003 0.0007 0.0008 0.0004 0.0005 0.0002 0.0008 0.0001 0.0004 0.0002
Nucleus 0.0108 0.0096 0.0124 0.0084 0.0255 0.0139 0.008 0.0108 0.0115 0.0214 0.0168 0.0154 0.0107 0.0105 0.0197 0.0197 0.0118 0.0143
Peroxisomes 0.001 0.0002 0.0012 0.0015 0.0007 0.0005 0.0154 0.0004 0.0016 0.001 0.0123 0.0033 0.0012 0.0003 0.0002 0.0003 0.0007 0.0002
Punctate Nuclear 0.0157 0.0032 0.0064 0.0061 0.0075 0.0109 0.0128 0.0032 0.005 0.0175 0.0095 0.0084 0.0036 0.0035 0.0096 0.0034 0.0088 0.0035
Vacuole 0.0029 0.0016 0.0015 0.002 0.0009 0.0011 0.0025 0.0014 0.001 0.0015 0.0036 0.0008 0.0017 0.0014 0.0014 0.001 0.0014 0.0009
Vacuole Periphery 0.0007 0.0002 0.0003 0.0007 0.0003 0.0002 0.0009 0.0002 0.0002 0.0008 0.0011 0.0003 0.0003 0.0002 0.0003 0.0002 0.0003 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 15.5166 10.2748 12.9613 14.0952 11.1767 6.8925 11.168 11.5694 14.0913 15.0323
Translational Efficiency 1.1444 1.6316 1.2764 1.4037 1.4845 2.1716 1.3456 1.3218 1.2703 1.3827

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
145 910 1321 966 2264 745 295 1062 2409 1655 1616 2028

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 846.89 943.71 1187.81 1006.30 823.23 874.21 1112.72 984.36 824.65 912.42 1174.10 994.81
Standard Deviation 94.81 143.93 158.36 134.54 100.27 113.59 137.21 148.33 100.11 135.63 157.41 142.35
Intensity Change Log 2 — 0.156169 0.488058 0.248814 — 0.086684 0.434723 0.257890 — 0.122337 0.462015 0.253295

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000319 0.000287 0.000333 0.000296 0.000142 0.000459 0.000502 0.001356 0.000153 0.000365 0.000364 0.000851
Bud Neck 0.005544 0.003538 0.001428 0.002382 0.004131 0.007418 0.002079 0.003921 0.004216 0.005285 0.001547 0.003188
Bud Site 0.008808 0.012812 0.002711 0.013758 0.004955 0.014425 0.006779 0.039525 0.005187 0.013538 0.003453 0.027251
Cell Periphery 0.000197 0.000139 0.000065 0.000120 0.000085 0.000140 0.000259 0.000295 0.000092 0.000140 0.000100 0.000212
Cytoplasm 0.530755* 0.684676* 0.540688* 0.637180* 0.699576* 0.552145* 0.424170* 0.657444* 0.689415* 0.625017* 0.519418* 0.647791*
Cytoplasmic Foci 0.252633* 0.128621 0.003358 0.014534 0.117250 0.215977* 0.027302 0.014836 0.125398 0.167945 0.007729 0.014692
Eisosomes 0.000148 0.000031 0.000006 0.000023 0.000041 0.000073 0.000104 0.000024 0.000047 0.000050 0.000024 0.000024
Endoplasmic Reticulum 0.002636 0.001428 0.000869 0.001585 0.001212 0.000797 0.005687 0.003206 0.001297 0.001144 0.001749 0.002434
Endosome 0.026263 0.010228 0.000379 0.003767 0.004239 0.025648 0.007832 0.006893 0.005565 0.017169 0.001739 0.005404
Golgi 0.001791 0.001765 0.000081 0.003490 0.000863 0.005532 0.000152 0.002764 0.000919 0.003461 0.000094 0.003110
Lipid Particles 0.006172 0.001751 0.000320 0.000847 0.001646 0.002780 0.003418 0.000675 0.001918 0.002214 0.000885 0.000757
Mitochondria 0.000807 0.001235 0.000391 0.000342 0.001225 0.004159 0.000172 0.000995 0.001200 0.002551 0.000351 0.000684
Mitotic Spindle 0.000272 0.005089 0.004052 0.024063 0.000269 0.010186 0.003318 0.014966 0.000269 0.007384 0.003918 0.019299
None 0.001005 0.003115 0.007007 0.003844 0.002538 0.001696 0.014132 0.008937 0.002445 0.002476 0.008308 0.006511
Nuclear Periphery 0.000259 0.000270 0.000609 0.000706 0.000185 0.000181 0.001290 0.000492 0.000189 0.000230 0.000734 0.000594
Nuclear Periphery Foci 0.001663 0.002594 0.000925 0.003160 0.000658 0.001067 0.008619 0.001548 0.000718 0.001907 0.002330 0.002316
Nucleolus 0.000377 0.000372 0.000092 0.000200 0.000414 0.000907 0.000433 0.000128 0.000412 0.000613 0.000154 0.000162
Nucleus 0.059580 0.068412 0.415591* 0.122222 0.116172 0.040953 0.198849* 0.147420* 0.112765 0.056051 0.376025* 0.135417*
Peroxisomes 0.003090 0.002473 0.000239 0.000183 0.000825 0.005752 0.000378 0.000920 0.000962 0.003949 0.000264 0.000569
Vacuole 0.096531 0.070030 0.020800 0.165372 0.042926 0.107787 0.294306* 0.091490 0.046152 0.087026 0.070729 0.126682
Vacuole Periphery 0.001151 0.001132 0.000056 0.001928 0.000649 0.001918 0.000220 0.002162 0.000679 0.001486 0.000086 0.002051

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.29 -0.88 0.04 -0.58 1.12 -2.63 -7.96 -3.21 -1.47 -0.39 -3.20 -3.42 -3.70 -1.68 -0.53
Bud Neck 1.95 3.75 3.46 2.47 -0.87 -3.77 1.01 2.27 5.23 1.40 -1.96 4.00 3.41 5.54 -0.44
Bud Site -1.80 3.84 0.62 2.43 -3.55 -6.88 -2.44 -3.62 0.26 -1.38 -6.93 0.78 -4.00 1.47 -4.33
Cell Periphery 1.19 4.58 4.41 1.02 -0.39 -3.10 -9.18 -1.35 0.15 3.20 -1.89 -3.93 -1.21 0.79 0.41
Cytoplasm -6.64 1.76 2.04 15.90 0.71 13.13 24.70 20.17 2.97 -10.97 8.64 30.26 25.87 13.37 -4.17
Cytoplasmic Foci 7.82 16.65 15.96 21.84 -12.91 -12.52 28.87 34.05 27.94 3.84 -7.77 39.05 36.70 34.41 -9.87
Eisosomes 5.00 6.00 5.06 0.48 -17.65 -4.48 -9.77 3.51 5.62 10.90 -0.89 7.32 6.55 5.36 -1.97
Endoplasmic Reticulum 0.72 0.81 0.67 -0.26 -1.67 1.82 -9.09 -1.30 -2.67 7.54 0.51 -3.88 -1.17 -1.83 2.93
Endosome 2.29 3.74 3.52 6.69 -4.52 -12.04 -4.22 3.00 12.66 5.75 -10.01 7.24 5.54 13.73 -0.93
Golgi 0.09 2.92 1.34 1.36 -1.38 -4.60 8.88 0.72 4.65 -1.90 -4.64 10.10 0.74 4.54 -2.26
Lipid Particles 2.49 3.24 2.83 1.55 -9.62 -3.98 -5.92 3.59 6.11 7.79 -1.20 4.68 4.39 4.73 -0.98
Mitochondria -0.66 0.74 1.18 2.10 4.31 -1.88 2.79 2.13 2.45 -1.99 -1.64 2.30 2.45 2.99 1.02
Mitotic Spindle -2.88 -4.33 -5.96 -3.00 -3.46 -3.55 -2.73 -5.83 -0.89 -2.41 -4.57 -5.04 -8.34 -2.76 -4.34
None -3.13 -15.18 -7.33 -1.52 6.16 2.20 -2.19 -7.52 -7.76 0.42 0.09 -8.47 -8.64 -6.30 2.02
Nuclear Periphery -0.26 -12.73 -14.93 -12.17 -5.48 0.01 -17.81 -18.63 -17.49 7.05 -1.24 -25.69 -26.23 -18.83 -1.96
Nuclear Periphery Foci -2.20 0.88 -5.21 -1.39 -8.00 -4.52 -11.08 -10.60 -6.06 9.28 -5.40 -11.26 -14.80 -2.52 0.99
Nucleolus -0.35 4.83 2.88 3.92 -4.34 -2.28 -0.18 1.91 4.00 3.97 -1.51 1.87 1.82 4.93 -0.41
Nucleus -1.40 -41.39 -10.99 -13.53 35.90 22.21 -9.35 -12.37 -25.95 3.30 17.33 -44.41 -13.05 -27.01 32.99
Peroxisomes 0.69 3.94 4.09 5.43 0.73 -5.72 3.60 3.23 6.33 0.52 -6.39 4.88 5.50 8.15 0.15
Vacuole 2.16 5.48 -15.54 -24.32 -29.42 -12.08 -19.44 -24.62 -13.43 6.71 -12.60 -10.94 -37.27 -26.68 -22.59
Vacuole Periphery 0.08 2.19 1.20 1.92 -1.60 -4.20 2.32 0.41 3.91 -1.12 -3.32 3.28 0.72 4.02 -2.17
Standard name
Human Ortholog
Description Thiamine pyrophosphokinase; phosphorylates thiamine to produce the coenzyme thiamine pyrophosphate (thiamine diphosphate)
Localization
Cell Percentages cytoplasm (96%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Thi80

Thi80


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Thi80-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available