Standard name | |
---|---|
Human Ortholog | |
Description | Thiamine pyrophosphokinase; phosphorylates thiamine to produce the coenzyme thiamine pyrophosphate (thiamine diphosphate) |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
Bud | 0 | 0 | 0 | 0 | 1 | 0 | 4 | 5 | 3 | 10 | 8 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 7 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 4 | 2 | 3 | 4 | 5 | 5 | 1 | 0 | 3 | 2 | 5 | 0 | 2 | 5 | 9 | 9 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 153 | 182 | 186 | 232 | 311 | 297 | 337 | 331 | 216 | 220 | 225 | 259 | 333 | 465 | 552 | 176 | 242 | 182 | 157 | 171 | 166 | 269 | 367 | 433 |
Endoplasmic Reticulum | 2 | 4 | 0 | 0 | 1 | 2 | 1 | 1 | 3 | 0 | 0 | 1 | 4 | 0 | 2 | 6 | 8 | 4 | 0 | 0 | 2 | 0 | 3 | 4 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 2 | 0 | 0 | 3 | 1 | 1 | 1 | 2 | 2 |
Golgi | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 1 |
Mitochondria | 8 | 1 | 0 | 7 | 3 | 32 | 122 | 63 | 102 | 80 | 131 | 72 | 0 | 0 | 0 | 1 | 2 | 1 | 1 | 2 | 6 | 0 | 4 | 4 |
Nucleus | 1 | 1 | 2 | 2 | 2 | 3 | 9 | 10 | 12 | 10 | 9 | 14 | 2 | 3 | 1 | 0 | 2 | 3 | 0 | 2 | 1 | 0 | 0 | 1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 |
Nucleolus | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 1 |
Vac/Vac Membrane | 0 | 0 | 0 | 3 | 6 | 6 | 42 | 32 | 6 | 30 | 14 | 29 | 0 | 10 | 2 | 20 | 18 | 7 | 1 | 13 | 18 | 1 | 3 | 3 |
Unique Cell Count | 162 | 187 | 187 | 239 | 321 | 323 | 447 | 399 | 294 | 303 | 335 | 337 | 340 | 471 | 555 | 188 | 250 | 193 | 168 | 196 | 200 | 286 | 400 | 474 |
Labelled Cell Count | 165 | 188 | 192 | 249 | 328 | 344 | 522 | 449 | 343 | 350 | 390 | 382 | 345 | 479 | 561 | 212 | 281 | 208 | 168 | 196 | 200 | 286 | 400 | 474 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.2 | 7.4 | 6.3 | 6.4 | 6.4 | 5.4 | 5.1 | 5.6 | 4.9 | 5.1 | 4.8 | 5.3 | 7.4 | 7.3 | 7.0 | 9.8 | 9.2 | 8.8 | 7.0 | 7.1 | 7.6 |
Std Deviation (1e-4) | 0.7 | 1.1 | 0.8 | 1.3 | 1.3 | 1.0 | 1.3 | 1.0 | 1.1 | 1.3 | 1.0 | 1.1 | 0.9 | 0.9 | 0.9 | 2.0 | 1.5 | 1.7 | 1.1 | 1.1 | 1.6 |
Intensity Change (Log2) | – | – | – | 0.03 | 0.02 | -0.21 | -0.31 | -0.17 | -0.36 | -0.29 | -0.4 | -0.25 | 0.23 | 0.21 | 0.15 | 0.64 | 0.55 | 0.49 | 0.15 | 0.17 | 0.27 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.9 | 1.4 |
Cytoplasm | -1.8 | -1.9 | -3.7 | -7.3 | -5.8 | -7.5 | -7.7 | -8.6 | -6.9 | -1.4 | -0.8 | 0 | -3.1 | -1.9 | -2.9 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 4.4 | 7.9 | 5.8 | 9.1 | 7.7 | 0 | 6.8 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 1.9 | 0 | 0 | 2.0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 4.3 | 4.0 | 0 | 4.4 | 2.8 | 4.1 | 0 | 0 | 0 | 4.6 | 3.7 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.2917 | 1.9996 | 1.5895 | 0.9 | 1.0757 | 1.4846 | 2.0816 | 2.9341 | 2.4423 | 2.4259 | 1.966 | 2.6332 | 2.0892 | 2.9938 | 2.4763 | 2.5668 | 2.302 | 2.4842 |
Actin | 0.0108 | 0.0019 | 0.0019 | 0.003 | 0.0019 | 0.0021 | 0.0235 | 0.0005 | 0.0015 | 0.0011 | 0.0441 | 0.0074 | 0.0022 | 0.0005 | 0.0008 | 0.0004 | 0.007 | 0.0009 |
Bud | 0.0012 | 0.0061 | 0.0018 | 0.0011 | 0.0006 | 0.0023 | 0.0076 | 0.0015 | 0.0051 | 0.0101 | 0.0005 | 0.0023 | 0.0085 | 0.0093 | 0.0088 | 0.0002 | 0.0036 | 0.0025 |
Bud Neck | 0.0068 | 0.0003 | 0.0017 | 0.0006 | 0.0008 | 0.0016 | 0.0034 | 0.0005 | 0.0006 | 0.0012 | 0.0018 | 0.0056 | 0.0004 | 0.0003 | 0.0007 | 0.0005 | 0.004 | 0.0013 |
Bud Periphery | 0.0018 | 0.0015 | 0.0013 | 0.0006 | 0.0003 | 0.0005 | 0.0049 | 0.0004 | 0.0012 | 0.0035 | 0.0006 | 0.0012 | 0.0029 | 0.0028 | 0.0033 | 0.0001 | 0.0013 | 0.001 |
Bud Site | 0.004 | 0.0059 | 0.0073 | 0.0008 | 0.0002 | 0.0002 | 0.0034 | 0.0096 | 0.0062 | 0.0008 | 0.001 | 0.0007 | 0.0055 | 0.0062 | 0.0047 | 0.0002 | 0.001 | 0.0004 |
Cell Periphery | 0.0002 | 0.0002 | 0.0001 | 0 | 0 | 0 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0006 | 0.0002 | 0 | 0.0001 | 0.0001 |
Cytoplasm | 0.6583 | 0.8163 | 0.719 | 0.7565 | 0.8117 | 0.7991 | 0.6375 | 0.8037 | 0.7681 | 0.7264 | 0.6806 | 0.7945 | 0.844 | 0.8657 | 0.8443 | 0.8536 | 0.8311 | 0.8784 |
Cytoplasmic Foci | 0.0171 | 0.0072 | 0.0185 | 0.019 | 0.0168 | 0.0155 | 0.0321 | 0.0141 | 0.0128 | 0.014 | 0.0337 | 0.0227 | 0.0119 | 0.0092 | 0.0079 | 0.0092 | 0.0192 | 0.0116 |
Eisosomes | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0.0003 | 0 | 0 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0054 | 0.0027 | 0.0023 | 0.0113 | 0.0029 | 0.0022 | 0.0054 | 0.0027 | 0.0019 | 0.0021 | 0.0046 | 0.0014 | 0.0028 | 0.0028 | 0.0028 | 0.0028 | 0.004 | 0.0016 |
Endosome | 0.0237 | 0.0037 | 0.0036 | 0.0155 | 0.0055 | 0.0063 | 0.0272 | 0.0054 | 0.0041 | 0.0111 | 0.0394 | 0.0071 | 0.0046 | 0.0039 | 0.0037 | 0.0049 | 0.0107 | 0.0052 |
Golgi | 0.0023 | 0.0003 | 0.0004 | 0.001 | 0.0003 | 0.0002 | 0.0063 | 0.0004 | 0.0004 | 0.0007 | 0.0049 | 0.0011 | 0.0004 | 0.0002 | 0.0002 | 0.0002 | 0.0022 | 0.0002 |
Lipid Particles | 0.002 | 0.0001 | 0.0002 | 0.0006 | 0.0003 | 0.0002 | 0.0071 | 0.0001 | 0.0002 | 0.0007 | 0.0035 | 0.0018 | 0.001 | 0.0001 | 0.0001 | 0.0001 | 0.0007 | 0.0001 |
Mitochondria | 0.0012 | 0.0005 | 0.0004 | 0.0008 | 0.0005 | 0.0004 | 0.0047 | 0.0005 | 0.0006 | 0.001 | 0.0032 | 0.0011 | 0.0007 | 0.0005 | 0.0006 | 0.0005 | 0.0005 | 0.0004 |
None | 0.2266 | 0.1361 | 0.2175 | 0.1602 | 0.1192 | 0.1403 | 0.187 | 0.1425 | 0.1756 | 0.1677 | 0.1313 | 0.1206 | 0.0953 | 0.0796 | 0.0875 | 0.0999 | 0.0885 | 0.0757 |
Nuclear Periphery | 0.0056 | 0.0022 | 0.002 | 0.01 | 0.0039 | 0.002 | 0.0089 | 0.0018 | 0.002 | 0.0164 | 0.0063 | 0.0036 | 0.0018 | 0.0023 | 0.0026 | 0.0026 | 0.0027 | 0.0016 |
Nucleolus | 0.0015 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0004 | 0.001 | 0.0002 | 0.0003 | 0.0007 | 0.0008 | 0.0004 | 0.0005 | 0.0002 | 0.0008 | 0.0001 | 0.0004 | 0.0002 |
Nucleus | 0.0108 | 0.0096 | 0.0124 | 0.0084 | 0.0255 | 0.0139 | 0.008 | 0.0108 | 0.0115 | 0.0214 | 0.0168 | 0.0154 | 0.0107 | 0.0105 | 0.0197 | 0.0197 | 0.0118 | 0.0143 |
Peroxisomes | 0.001 | 0.0002 | 0.0012 | 0.0015 | 0.0007 | 0.0005 | 0.0154 | 0.0004 | 0.0016 | 0.001 | 0.0123 | 0.0033 | 0.0012 | 0.0003 | 0.0002 | 0.0003 | 0.0007 | 0.0002 |
Punctate Nuclear | 0.0157 | 0.0032 | 0.0064 | 0.0061 | 0.0075 | 0.0109 | 0.0128 | 0.0032 | 0.005 | 0.0175 | 0.0095 | 0.0084 | 0.0036 | 0.0035 | 0.0096 | 0.0034 | 0.0088 | 0.0035 |
Vacuole | 0.0029 | 0.0016 | 0.0015 | 0.002 | 0.0009 | 0.0011 | 0.0025 | 0.0014 | 0.001 | 0.0015 | 0.0036 | 0.0008 | 0.0017 | 0.0014 | 0.0014 | 0.001 | 0.0014 | 0.0009 |
Vacuole Periphery | 0.0007 | 0.0002 | 0.0003 | 0.0007 | 0.0003 | 0.0002 | 0.0009 | 0.0002 | 0.0002 | 0.0008 | 0.0011 | 0.0003 | 0.0003 | 0.0002 | 0.0003 | 0.0002 | 0.0003 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 15.5166 | 10.2748 | 12.9613 | 14.0952 | 11.1767 | 6.8925 | 11.168 | 11.5694 | 14.0913 | 15.0323 |
Translational Efficiency | 1.1444 | 1.6316 | 1.2764 | 1.4037 | 1.4845 | 2.1716 | 1.3456 | 1.3218 | 1.2703 | 1.3827 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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