Standard name
Human Ortholog
Description Autophagy receptor with a role in endoplasmic reticulum degradation; involved specifically in autophagy of cortical and cytoplasmic ER in response to nitrogen starvation or rapamycin treatment; localizes to the cortical and cytoplasmic ER; similar to human FAM134B, which is also involved in ER autophagy and is associated with sensory neuropathy
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.08 0 0.12 0 – 0.12 0 – 0 0.05 0.06 0 0 0 0 0.28 0.3 0.2 0.09 0.06 0.14 0.11 0.14 0.11
Bud 0 0 0 0 – 0 0 – 0.07 0 0 0 0 0 0 0.07 0.07 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.06 0 0.06 0 – 0 0.05 – 0 0 0 0 0 0 0 0.26 0.38 0.29 0 0.05 0.08 0.05 0.07 0.08
Cytoplasm 0.69 0.92 0.73 0.88 – 0.5 0.51 – 0.3 0.24 0.26 0.35 0.95 0.96 0.94 0.45 0.33 0.45 0.25 0.58 0.17 0.48 0.37 0.33
Endoplasmic Reticulum 0.13 0 0.21 0 – 0.1 0 – 0.12 0.11 0.07 0.09 0 0 0 0 0 0 0.12 0 0.2 0.12 0.22 0.22
Endosome 0 0 0 0 – 0.1 0.07 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 – 0 0 0 0 0 0 0 0 0.07 0.06 0 0 0 0 0 0
Mitochondria 0.09 0 0 0 – 0.14 0.33 – 0.66 0.63 0.64 0.57 0 0 0 0 0.06 0 0.36 0.13 0.26 0.11 0.11 0.12
Nucleus 0 0 0 0 – 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0.05 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 – 0 0.1 – 0 0.05 0.11 0.05 0 0 0 0 0 0 0.07 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 8 1 4 1 – 9 2 – 4 4 5 4 0 2 0 45 41 29 13 14 9 26 28 32
Bud 2 2 1 0 – 1 3 – 8 3 4 4 0 0 0 12 10 4 2 4 0 4 3 7
Bud Neck 0 1 0 0 – 1 0 – 0 1 0 1 1 0 0 0 0 0 5 3 1 7 2 10
Bud Site 0 0 0 0 – 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 6 3 2 2 – 1 5 – 3 1 3 2 4 6 8 41 52 41 3 12 5 11 15 23
Cytoplasm 68 196 24 42 – 36 46 – 36 20 23 33 148 176 238 72 45 64 37 137 12 121 73 97
Endoplasmic Reticulum 13 1 7 2 – 7 3 – 14 9 6 8 5 7 10 6 6 3 17 6 13 29 44 63
Endosome 0 1 1 0 – 7 6 – 1 3 3 1 0 0 2 4 2 3 2 3 2 2 1 5
Golgi 0 0 0 0 – 1 0 – 1 0 0 0 1 1 1 1 10 9 0 1 0 1 0 0
Mitochondria 9 8 0 2 – 10 30 – 78 53 57 54 0 0 1 6 8 2 54 31 18 28 21 35
Nucleus 1 4 0 0 – 3 0 – 0 0 0 2 0 0 0 3 0 0 0 2 0 2 1 0
Nuclear Periphery 0 0 0 0 – 0 0 – 0 0 2 2 0 0 0 0 0 0 0 0 1 1 1 0
Nucleolus 0 0 0 0 – 3 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 – 1 0 1 0 0 0 1 1 0 0 0 0 0 0 0 1
SpindlePole 0 0 1 0 – 2 5 – 0 1 0 1 0 0 0 2 3 4 0 2 0 1 1 1
Vac/Vac Membrane 0 0 0 0 – 2 9 – 5 4 10 5 1 2 2 4 3 2 11 9 1 10 5 11
Unique Cell Count 99 212 33 48 72 91 119 84 89 94 155 184 252 160 136 143 149 236 71 253 202 294
Labelled Cell Count 107 217 40 49 83 109 151 99 114 117 160 194 263 197 180 161 149 236 71 253 202 294


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.6 3.9 5.9 5.8 – 6.2 5.4 – 5.3 5.6 5.6 5.5 5.9 6.0 6.3 10.9 14.4 13.6 6.4 7.1 6.9
Std Deviation (1e-4) 0.6 0.8 1.4 2.7 – 2.3 1.1 – 1.4 1.6 1.6 2.1 0.9 1.4 1.8 2.3 2.9 2.6 1.8 2.3 2.1
Intensity Change (Log2) – – – -0.01 – 0.08 -0.11 – -0.16 -0.08 -0.07 -0.11 0.01 0.04 0.1 0.9 1.3 1.21 0.13 0.27 0.22


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.4126 0.1331 0.1463 0.1411 -0.2453 0.5388 7.3395 5.169 5.5861 5.1489 4.9032 5.3786 3.8409 2.6054 2.6653 2.6526 2.973 2.4592
Actin 0.1665 0.0026 0.0084 0.0004 0.0002 0.0123 0.0906 0.0018 0.0022 0.0277 0.0719 0.0111 0.0356 0.007 0.0114 0.0376 0.0045 0.0035
Bud 0.0031 0.0022 0.0016 0.0004 0.0001 0.0028 0.0018 0.0059 0.0028 0.003 0.0004 0.0016 0.0019 0.0058 0.0111 0.0002 0.0028 0.0004
Bud Neck 0.0466 0.0004 0.0005 0.0001 0.0002 0.0081 0.0135 0.0019 0.0029 0.0006 0.0004 0.0118 0.0157 0.0011 0.002 0.001 0.0035 0.0015
Bud Periphery 0.0035 0.0022 0.0035 0.0008 0.0002 0.0028 0.0048 0.0049 0.0023 0.0183 0.0007 0.0026 0.0064 0.0035 0.0254 0.0003 0.003 0.001
Bud Site 0.0098 0.0062 0.0017 0.0002 0 0.0007 0.0141 0.0251 0.013 0.002 0.0007 0.0014 0.0041 0.0293 0.0125 0.0011 0.017 0.0003
Cell Periphery 0.0023 0.0005 0.0011 0.0001 0.0001 0.0003 0.0022 0.0008 0.0013 0.0006 0.0003 0.0005 0.0014 0.0011 0.0007 0.0002 0.0003 0.0003
Cytoplasm 0.085 0.1295 0.1159 0.1841 0.3081 0.2307 0.2168 0.1877 0.2123 0.2254 0.2981 0.312 0.1798 0.1484 0.1285 0.0747 0.1902 0.2181
Cytoplasmic Foci 0.0282 0.0142 0.0124 0.012 0.0107 0.0242 0.0462 0.0129 0.0158 0.0197 0.0104 0.0146 0.0721 0.0136 0.0163 0.0056 0.0537 0.0108
Eisosomes 0.0024 0.0003 0.0004 0 0 0.0002 0.0009 0.0002 0.0002 0.0002 0.0003 0.0002 0.0005 0.0002 0.0002 0.0001 0.0001 0.0001
Endoplasmic Reticulum 0.0198 0.0033 0.0049 0.0015 0.001 0.0192 0.0123 0.0046 0.0048 0.0101 0.0022 0.0043 0.0116 0.0017 0.0066 0.007 0.0212 0.0033
Endosome 0.0607 0.0059 0.0102 0.0062 0.0033 0.0146 0.0292 0.0057 0.0169 0.0228 0.0044 0.0087 0.0612 0.0058 0.012 0.0182 0.0317 0.0052
Golgi 0.0175 0.0005 0.0016 0.0006 0.0001 0.0036 0.0131 0.0008 0.001 0.0041 0.0016 0.0028 0.0156 0.0016 0.0045 0.0022 0.0078 0.0058
Lipid Particles 0.027 0.0013 0.0061 0.0003 0.0001 0.0016 0.0198 0.0015 0.0007 0.0014 0.0004 0.008 0.0332 0.0007 0.0013 0.0096 0.0178 0.0012
Mitochondria 0.0291 0.0005 0.0013 0.0008 0.0003 0.0018 0.0061 0.0008 0.0009 0.01 0.0015 0.0036 0.0107 0.0009 0.017 0.0047 0.0014 0.0008
None 0.2748 0.8232 0.8088 0.788 0.6716 0.6469 0.4447 0.7338 0.7053 0.6191 0.6035 0.6069 0.5034 0.7711 0.7169 0.7958 0.5889 0.7399
Nuclear Periphery 0.0419 0.0014 0.006 0.0006 0.0005 0.0117 0.0186 0.001 0.0018 0.0045 0.0006 0.0008 0.0057 0.0007 0.015 0.0093 0.0011 0.0003
Nucleolus 0.0665 0.0003 0.001 0.0002 0.0001 0.0006 0.0014 0.0008 0.0007 0.0003 0.0001 0.0003 0.0022 0.0004 0.0016 0.0003 0.0004 0.0004
Nucleus 0.0335 0.0012 0.003 0.0009 0.0011 0.0061 0.0068 0.0032 0.0036 0.0028 0.0007 0.0023 0.0039 0.002 0.0043 0.0015 0.0006 0.0012
Peroxisomes 0.0442 0.0009 0.0026 0.0003 0.0001 0.0036 0.0353 0.0016 0.0005 0.0053 0.0005 0.0019 0.0125 0.0014 0.0038 0.0081 0.0469 0.002
Punctate Nuclear 0.0225 0.0013 0.0065 0.0006 0.0005 0.0044 0.0158 0.0022 0.0058 0.0016 0.0003 0.0026 0.0094 0.0015 0.0035 0.0004 0.001 0.0031
Vacuole 0.0101 0.002 0.0019 0.0015 0.0015 0.0029 0.0042 0.0025 0.0045 0.0187 0.0011 0.0015 0.0102 0.0019 0.0039 0.0128 0.0056 0.0007
Vacuole Periphery 0.005 0.0003 0.0007 0.0003 0.0002 0.0009 0.0016 0.0003 0.0007 0.0016 0.0003 0.0004 0.0029 0.0002 0.0014 0.0092 0.0006 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 8.5868 15.3522 4.7467 8.8074 8.3499 17.1641 16.1693 17.4611 10.2539 16.8911
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
77 465 453 1245 1705 2239 1685 360 1782 2704 2138 1605

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 692.49 793.20 948.30 846.76 655.37 848.10 841.73 953.26 656.97 838.66 864.31 870.65
Standard Deviation 91.46 216.78 162.46 143.00 79.11 101.28 122.38 179.80 80.04 130.40 138.90 158.39
Intensity Change Log 2 — 0.195891 0.453550 0.290160 — 0.371925 0.361048 0.540560 — 0.284168 0.409313 0.417342

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.006338 0.005317 0.042442 0.048118 0.001003 0.003204 0.019773 0.108701* 0.001233 0.003567 0.024577 0.061706
Bud Neck 0.022221 0.048830 0.021082 0.031978 0.022151 0.042877 0.032293 0.033612 0.022154 0.043901 0.029918 0.032345
Bud Site 0.027501 0.043545 0.125046* 0.079432 0.009297 0.025242 0.052579 0.131999* 0.010084 0.028389 0.067933 0.091223
Cell Periphery 0.014147 0.001832 0.035584 0.009307 0.000579 0.001733 0.007653 0.069959 0.001165 0.001750 0.013571 0.022911
Cytoplasm 0.383028* 0.274827* 0.233878* 0.252477* 0.422392* 0.435828* 0.422297* 0.207608* 0.420691* 0.408141* 0.382374* 0.242413*
Cytoplasmic Foci 0.245219* 0.351624* 0.094426 0.147965 0.285236* 0.215136* 0.098918 0.059430 0.283507* 0.238608* 0.097966 0.128106
Eisosomes 0.007862 0.002564 0.006000 0.009912 0.001511 0.005007 0.006993 0.038651 0.001786 0.004587 0.006782 0.016358
Endoplasmic Reticulum 0.029677 0.011446 0.245545* 0.037414 0.010977 0.012803 0.046797 0.133392* 0.011785 0.012569 0.088908 0.058942
Endosome 0.017395 0.044832 0.019547 0.038674 0.016004 0.012904 0.008952 0.007991 0.016064 0.018394 0.011197 0.031792
Golgi 0.046491 0.039892 0.016220 0.022028 0.015103 0.020607 0.006661 0.012477 0.016459 0.023923 0.008686 0.019885
Lipid Particles 0.034998 0.023315 0.043673 0.088892 0.032423 0.036477 0.024609 0.051665 0.032535 0.034213 0.028649 0.080542
Mitochondria 0.050019 0.011853 0.001335 0.007991 0.006864 0.010865 0.001302 0.000793 0.008729 0.011035 0.001309 0.006376
Mitotic Spindle 0.000423 0.006692 0.000628 0.013262 0.000740 0.002329 0.002720 0.001665 0.000727 0.003079 0.002277 0.010660
None 0.003399 0.001348 0.000329 0.002718 0.005014 0.002591 0.002771 0.000794 0.004945 0.002377 0.002254 0.002286
Nuclear Periphery 0.000280 0.000367 0.000251 0.001182 0.000436 0.000273 0.000752 0.000156 0.000430 0.000289 0.000646 0.000952
Nuclear Periphery Foci 0.000598 0.001394 0.012126 0.005654 0.001035 0.000665 0.005399 0.003735 0.001016 0.000791 0.006824 0.005224
Nucleolus 0.000350 0.001381 0.000150 0.001553 0.000883 0.000822 0.000388 0.000087 0.000860 0.000918 0.000338 0.001224
Nucleus 0.041933 0.031685 0.023038 0.042544 0.113402 0.110901 0.126696 0.032505 0.110313 0.097278 0.104733 0.040292
Peroxisomes 0.021359 0.029400 0.006674 0.019182 0.008645 0.015901 0.006679 0.016589 0.009194 0.018222 0.006678 0.018601
Vacuole 0.041950 0.064625 0.070563 0.137020 0.045035 0.042469 0.123487 0.087548 0.044901 0.046279 0.112274 0.125924
Vacuole Periphery 0.004812 0.003232 0.001461 0.002699 0.001271 0.001367 0.002280 0.000643 0.001424 0.001688 0.002106 0.002237

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.28 -6.62 -7.71 -10.49 -0.62 -6.12 -10.83 -10.72 -10.48 -8.75 -5.89 -14.24 -15.88 -15.19 -8.89
Bud Neck -4.18 0.31 -1.71 3.44 -3.12 -8.92 -3.60 -3.08 2.91 -0.48 -9.91 -3.13 -3.75 4.94 -0.90
Bud Site -1.95 -8.66 -5.74 -5.19 4.91 -8.38 -12.64 -10.67 -9.18 -6.82 -10.10 -17.19 -17.41 -12.76 -4.16
Cell Periphery 2.11 -2.91 0.93 -5.01 6.13 -6.31 -9.77 -9.64 -9.47 -8.64 -1.92 -11.04 -10.49 -10.24 -3.90
Cytoplasm 3.73 5.11 4.81 1.99 -1.16 -1.81 0.73 16.91 18.57 16.04 1.67 5.28 21.84 22.32 16.44
Cytoplasmic Foci -4.71 7.34 4.79 17.59 -7.21 11.02 31.28 28.95 21.85 5.82 7.30 32.92 24.56 19.90 -6.21
Eisosomes 2.15 0.76 -0.49 -5.88 -2.51 -8.88 -8.55 -9.18 -8.24 -7.80 -7.84 -9.11 -10.96 -8.52 -6.82
Endoplasmic Reticulum 2.36 -13.63 -0.72 -6.43 14.75 -1.14 -10.49 -10.08 -9.92 -6.99 -0.52 -17.42 -11.99 -11.87 5.59
Endosome -5.55 -0.63 -5.71 1.59 -7.10 3.01 7.01 6.22 4.53 0.95 -1.87 5.02 -7.93 -6.70 -11.42
Golgi 0.83 3.96 3.35 4.61 -1.87 -4.11 7.38 1.91 5.01 -3.49 -5.28 6.97 -1.19 3.00 -6.33
Lipid Particles 2.14 -1.30 -7.53 -12.22 -7.07 -1.68 3.89 -2.86 -2.20 -4.40 -0.73 2.16 -10.92 -10.79 -12.34
Mitochondria 2.80 3.60 3.14 1.86 -5.01 -3.71 6.58 7.18 14.60 2.54 -1.90 7.31 2.03 4.46 -4.96
Mitotic Spindle -3.07 -0.80 -6.12 -2.34 -6.05 -2.43 -2.07 -1.21 -0.56 -0.41 -3.52 -1.96 -5.97 -4.60 -5.00
None 0.75 1.24 0.32 -1.17 -2.99 3.54 2.33 5.62 3.53 1.99 3.88 3.18 3.28 0.36 -0.04
Nuclear Periphery -0.61 0.26 -5.19 -3.98 -5.73 2.27 -3.33 3.92 5.61 7.92 1.92 -2.68 -3.85 -5.24 -2.24
Nuclear Periphery Foci -3.27 -9.37 -9.76 -7.90 4.23 2.50 -11.77 -3.35 -3.82 1.90 1.64 -14.53 -9.55 -10.03 1.67
Nucleolus -1.81 2.03 -2.63 -0.43 -3.03 0.58 8.13 14.83 7.45 8.93 -0.46 9.21 -1.28 -1.09 -2.54
Nucleus 0.89 1.56 -0.05 -2.88 -5.55 0.57 -3.14 15.73 16.37 17.86 3.30 0.87 19.07 18.95 18.49
Peroxisomes -1.82 4.31 0.73 3.56 -9.47 -8.19 3.03 -4.09 0.03 -5.32 -9.76 4.06 -8.38 0.07 -11.26
Vacuole -1.67 -2.35 -7.91 -11.62 -9.87 1.29 -20.30 -6.07 -6.38 3.03 -0.58 -20.04 -18.59 -18.63 -2.94
Vacuole Periphery 1.21 2.68 1.81 1.31 -2.29 -0.56 -1.20 2.01 2.51 2.02 -1.42 -0.97 -2.01 -1.27 -0.19
Standard name
Human Ortholog
Description Autophagy receptor with a role in endoplasmic reticulum degradation; involved specifically in autophagy of cortical and cytoplasmic ER in response to nitrogen starvation or rapamycin treatment; localizes to the cortical and cytoplasmic ER; similar to human FAM134B, which is also involved in ER autophagy and is associated with sensory neuropathy
Localization
Cell Percentages ER (24%), cytoplasm (16%), mixed (52%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Atg40

Atg40


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Atg40-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available