Standard name
Human Ortholog
Description SUMO E3 ligase; catalyzes sumoylation of Yku70p/80p and Sir4p promoting telomere anchoring to the nuclear envelope and regulating telomerase activity; DNA-bound form catalyzes a DNA-damaged triggered sumoylation wave resulting in multisite modification of several DNA repair proteins, enhancing interactions between these proteins and accelerating repair; sumoylates Cse4p, a prerequisite for STUbL-mediated Ub-dependent degradation; role in telomere length maintenance
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Neck 0 0 0.09 0 0 0 0 0 0 – 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – 0 0 0 0 0 0
Cytoplasm 0.14 0.12 0.36 0.34 0.34 0.34 0.3 0.47 0.31 – 0.24 0.34 0.13 0.27 0.4 – – – 0.08 0.08 0.1 0.08 0.1 0.16
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – 0 0 0 0 0 0
Mitochondria 0.19 0.12 0 0.21 0.11 0.42 0.4 0.21 0.61 – 0.73 0.51 0.2 0.05 0.07 – – – 0 0 0.06 0.08 0.08 0.08
Nucleus 0.71 0.61 0.51 0.48 0.5 0.36 0.39 0.33 0.24 – 0.24 0.25 0.72 0.55 0.39 – – – 0.59 0.6 0.4 0.33 0.23 0.14
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – 0 0 0 0 0.07 0.07
Vac/Vac Membrane 0.09 0.21 0 0 0.06 0 0 0 0 – 0 0 0.05 0.12 0.16 – – – 0.22 0.18 0.31 0.37 0.37 0.41
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 2 0 – 0 0 0 0 0 – – – 0 1 1 0 1 1
Bud 0 0 0 0 1 2 4 4 15 – 9 11 0 0 0 – – – 3 2 5 2 4 8
Bud Neck 2 0 26 6 12 6 16 16 14 – 4 11 0 0 0 – – – 1 3 4 2 5 6
Bud Site 0 0 0 2 0 2 10 1 7 – 2 6 0 0 0 – – – – – – – – –
Cell Periphery 0 1 0 0 1 0 1 0 0 – 0 0 0 0 0 – – – 0 0 0 0 0 0
Cytoplasm 36 30 107 83 95 135 146 179 125 – 73 124 28 60 109 – – – 25 22 54 8 26 41
Endoplasmic Reticulum 2 0 2 0 0 0 0 0 0 – 1 0 1 3 1 – – – 0 1 1 1 2 2
Endosome 0 9 1 2 3 1 0 0 1 – 0 0 1 7 6 – – – 5 6 3 0 7 3
Golgi 0 1 0 1 0 0 0 0 0 – 0 0 1 0 0 – – – 0 1 1 0 1 0
Mitochondria 49 31 13 52 30 165 190 79 249 – 225 186 43 12 19 – – – 6 6 28 8 21 20
Nucleus 187 155 150 119 141 141 188 124 99 – 73 91 153 124 106 – – – 196 175 208 38 64 38
Nuclear Periphery 0 0 0 0 0 0 0 1 2 – 1 0 0 1 0 – – – 0 1 0 0 1 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 1 0 0 0 0 – – – 3 3 1 1 3 1
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 2 2 10 10 8 7 11 13 7 – 5 13 0 2 1 – – – 12 6 20 3 20 18
Vac/Vac Membrane 25 52 11 7 18 16 13 13 15 – 4 9 10 26 45 – – – 74 52 161 42 102 108
Unique Cell Count 264 253 297 247 280 396 479 380 406 309 368 213 225 273 336 293 520 114 276 263
Labelled Cell Count 303 281 320 282 309 475 580 432 534 398 451 237 235 287 336 293 520 114 276 263


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.2 4.6 4.8 4.5 4.9 3.8 4.0 4.4 3.4 – 3.1 3.7 4.4 4.5 4.3 – – – 3.9 4.1 4.5
Std Deviation (1e-4) 0.5 1.1 0.7 1.3 1.0 0.8 0.9 1.2 0.9 – 0.9 0.7 1.2 1.2 0.9 – – – 1.4 1.4 1.6
Intensity Change (Log2) – – – -0.11 0.01 -0.34 -0.27 -0.15 -0.52 – -0.62 -0.39 -0.13 -0.11 -0.17 – – – -0.29 -0.24 -0.12


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.9809 -0.6316 -1.0161 -1.1588 -1.678 -0.8936 0.1519 0.4861 0.4494 0.316 0.1406 0.4235 -0.2911 -0.4861 -0.394 -0.6293 -0.333 -0.4096
Actin 0.0071 0.0004 0.0006 0.0002 0.0002 0.0002 0.012 0.0008 0.0024 0.0058 0.0013 0.0004 0.0077 0.0001 0.0016 0 0.0054 0.0022
Bud 0.0001 0.0001 0.0001 0 0 0 0.0003 0.0004 0.0007 0.0001 0.001 0 0.0002 0 0.0001 0 0.0003 0.0001
Bud Neck 0.0009 0.0005 0.0004 0.0003 0.0016 0.0032 0.0065 0.0009 0.0043 0.0004 0.0015 0.0015 0.0022 0.0003 0.0004 0.0004 0.0012 0.0021
Bud Periphery 0.0002 0.0001 0.0001 0 0.0001 0 0.0005 0.0004 0.0007 0.0002 0.0029 0.0001 0.0005 0 0.0004 0 0.001 0.0002
Bud Site 0.0023 0.0004 0.0003 0 0.0001 0.0001 0.0034 0.0034 0.0038 0.0002 0.0059 0.0001 0.0006 0.0005 0.0003 0 0.001 0.0001
Cell Periphery 0.0001 0 0 0 0 0 0.0002 0 0.0003 0 0.0004 0 0.0001 0 0 0 0.0001 0
Cytoplasm 0.0229 0.0513 0.0292 0.0263 0.0112 0.0197 0.0054 0.0316 0.0094 0.0151 0.0207 0.011 0.0118 0.0235 0.0171 0.0051 0.046 0.0096
Cytoplasmic Foci 0.0054 0.002 0.0029 0.002 0.001 0.0005 0.0033 0.001 0.0022 0.0006 0.0113 0.0011 0.0076 0.0005 0.0018 0.0002 0.0092 0.0013
Eisosomes 0.0002 0.0001 0 0 0 0 0.0001 0 0.0001 0.0001 0.0001 0 0.0001 0 0 0 0.0001 0.0001
Endoplasmic Reticulum 0.0023 0.0024 0.0015 0.0007 0.0008 0.0013 0.0015 0.0007 0.0005 0.0025 0.009 0.0006 0.001 0.0005 0.0005 0.0001 0.0011 0.0002
Endosome 0.0093 0.0023 0.0032 0.0011 0.001 0.0009 0.0142 0.0011 0.0012 0.0144 0.0316 0.0013 0.0062 0.001 0.0039 0.0001 0.0128 0.0015
Golgi 0.0012 0.0002 0.0004 0.0001 0 0 0.006 0.0001 0.0006 0.0034 0.0036 0.0007 0.0018 0 0.0008 0 0.005 0.001
Lipid Particles 0.0039 0.0015 0.0031 0.0002 0.0003 0.0002 0.0054 0.0003 0.0015 0.0024 0.0097 0.0066 0.0083 0.0002 0.0016 0 0.0179 0.0039
Mitochondria 0.0014 0.0007 0.002 0.0002 0.0007 0.0002 0.0023 0.0004 0.0143 0.0044 0.0236 0.0055 0.0062 0.0003 0.001 0.0001 0.0321 0.0011
None 0.0647 0.0877 0.072 0.1226 0.0434 0.0335 0.0098 0.0318 0.0272 0.0543 0.0095 0.0257 0.0269 0.0876 0.0719 0.009 0.1035 0.0443
Nuclear Periphery 0.0235 0.0223 0.0172 0.0217 0.0262 0.0188 0.0074 0.0126 0.0076 0.0219 0.0236 0.0081 0.0123 0.0078 0.0087 0.0025 0.0198 0.01
Nucleolus 0.0088 0.0101 0.0077 0.0062 0.0086 0.0167 0.0113 0.0063 0.0069 0.0032 0.0186 0.0109 0.0079 0.0082 0.0101 0.0087 0.0106 0.0153
Nucleus 0.7842 0.7385 0.8091 0.7283 0.868 0.8426 0.8726 0.8829 0.8698 0.8603 0.7884 0.8817 0.836 0.8298 0.8455 0.9338 0.6193 0.8324
Peroxisomes 0.0044 0.0059 0.0079 0.0001 0 0 0.0041 0.0001 0.0024 0.0003 0.0082 0.0019 0.0137 0 0.0012 0 0.0292 0.0027
Punctate Nuclear 0.0551 0.0711 0.0408 0.0897 0.0359 0.0613 0.0312 0.0238 0.0429 0.0078 0.0205 0.0423 0.0445 0.0388 0.0319 0.0399 0.0817 0.0715
Vacuole 0.0016 0.0017 0.001 0.0002 0.0006 0.0006 0.0019 0.0013 0.0007 0.0007 0.0067 0.0004 0.0028 0.0006 0.0009 0 0.0016 0.0002
Vacuole Periphery 0.0006 0.0004 0.0003 0.0001 0.0003 0.0002 0.0004 0.0002 0.0003 0.0019 0.002 0.0002 0.0016 0.0001 0.0003 0 0.0012 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 8.8535 5.4271 4.6983 8.0678 6.8874 12.1353 9.0312 9.4133 10.4717 11.0547
Translational Efficiency 0.761 0.8412 1.0221 0.693 0.7936 0.6846 0.704 0.6893 0.6603 0.6629

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
172 1398 117 1159 1761 1485 430 2346 1933 2883 547 3505

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 597.91 743.89 1004.23 836.77 687.65 720.57 924.83 856.83 679.66 731.88 941.81 850.20
Standard Deviation 55.96 100.12 105.10 102.55 78.39 89.76 112.15 121.36 80.81 95.64 115.37 115.86
Intensity Change Log 2 — 0.315161 0.748089 0.484903 — 0.067464 0.427514 0.317335 — 0.187972 0.585501 0.397696

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000028 0.000287 0.001765 0.002316 0.000045 0.000406 0.001034 0.000551 0.000043 0.000348 0.001190 0.001135
Bud Neck 0.003444 0.007786 0.003815 0.007769 0.002742 0.014396 0.004523 0.018539 0.002804 0.011191 0.004371 0.014978
Bud Site 0.000421 0.003661 0.010339 0.021016 0.000631 0.007830 0.004144 0.024025 0.000613 0.005808 0.005469 0.023030
Cell Periphery 0.000144 0.000106 0.000246 0.000135 0.000102 0.000126 0.000105 0.000198 0.000106 0.000116 0.000135 0.000178
Cytoplasm 0.263365* 0.171823* 0.023546 0.147170* 0.184532* 0.179600* 0.029009 0.190350* 0.191547* 0.175829* 0.027840 0.176072*
Cytoplasmic Foci 0.094428 0.036924 0.000789 0.004820 0.032964 0.060684 0.000461 0.003485 0.038433 0.049163 0.000531 0.003926
Eisosomes 0.000088 0.000042 0.000079 0.000030 0.000035 0.000064 0.000039 0.000025 0.000040 0.000053 0.000048 0.000027
Endoplasmic Reticulum 0.000447 0.001934 0.020689 0.006537 0.000903 0.001727 0.009015 0.003830 0.000863 0.001827 0.011512 0.004725
Endosome 0.002394 0.004540 0.001187 0.004160 0.000933 0.004992 0.000626 0.002977 0.001063 0.004772 0.000746 0.003368
Golgi 0.000121 0.002849 0.000103 0.001124 0.000301 0.002702 0.000052 0.001221 0.000285 0.002773 0.000063 0.001189
Lipid Particles 0.007998 0.004734 0.001413 0.000631 0.004182 0.006223 0.000584 0.000538 0.004521 0.005501 0.000761 0.000569
Mitochondria 0.001839 0.008598 0.000943 0.001490 0.001947 0.011858 0.001344 0.003624 0.001938 0.010277 0.001258 0.002918
Mitotic Spindle 0.000019 0.006478 0.000134 0.014352 0.000793 0.010945 0.000684 0.038781 0.000724 0.008779 0.000566 0.030703
None 0.006642 0.013970 0.002917 0.006329 0.007287 0.006202 0.003238 0.010320 0.007229 0.009969 0.003170 0.009001
Nuclear Periphery 0.000937 0.002338 0.001683 0.002696 0.001754 0.001591 0.001639 0.004178 0.001681 0.001953 0.001649 0.003688
Nuclear Periphery Foci 0.000410 0.000696 0.000988 0.000620 0.000383 0.000588 0.000323 0.000950 0.000386 0.000640 0.000465 0.000841
Nucleolus 0.003062 0.006369 0.002170 0.000744 0.002412 0.006355 0.000819 0.000963 0.002470 0.006362 0.001108 0.000891
Nucleus 0.604234* 0.707167* 0.901279* 0.743866* 0.751812* 0.655658* 0.927567* 0.662222* 0.738681* 0.680635* 0.921944* 0.689219*
Peroxisomes 0.002211 0.003953 0.000329 0.000691 0.002256 0.010377 0.000983 0.000584 0.002252 0.007262 0.000843 0.000619
Vacuole 0.007490 0.014371 0.025388 0.032201 0.003791 0.016348 0.013711 0.030527 0.004120 0.015389 0.016208 0.031081
Vacuole Periphery 0.000278 0.001373 0.000199 0.001303 0.000193 0.001327 0.000100 0.002110 0.000201 0.001350 0.000121 0.001843

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.95 -7.72 -2.39 -1.79 1.74 -5.53 -7.03 -9.44 -1.33 3.64 -5.64 -9.54 -4.41 -2.26 2.88
Bud Neck -3.83 -2.00 -7.23 -1.51 -6.36 -7.13 -7.64 -13.79 -1.18 -5.97 -8.51 -7.98 -15.99 -2.56 -6.62
Bud Site -4.16 -1.36 -4.69 -2.76 -0.30 -5.77 -3.21 -9.33 -3.45 -5.88 -6.83 -2.95 -10.28 -5.02 -4.41
Cell Periphery 1.91 -1.26 1.25 -1.52 1.66 -2.06 -0.67 -3.12 -0.88 -2.18 -1.00 -1.70 -2.63 -1.92 0.41
Cytoplasm 4.67 12.77 7.94 7.73 -12.72 0.65 25.70 5.21 4.34 -23.04 2.36 28.89 9.03 7.48 -25.87
Cytoplasmic Foci 5.37 9.03 8.83 13.57 -3.34 -7.73 16.44 15.42 19.17 -4.92 -3.86 18.38 17.35 23.45 -6.19
Eisosomes 2.99 -0.66 2.37 -3.01 4.32 -7.81 -5.06 1.38 9.31 6.09 -4.92 -5.91 0.37 6.77 6.73
Endoplasmic Reticulum -11.65 -8.89 -20.48 -16.36 5.15 -7.30 -13.72 -19.52 -13.50 8.68 -11.07 -15.84 -27.10 -20.70 9.12
Endosome -2.61 0.87 0.37 3.56 -0.59 -7.12 0.45 -2.84 5.64 -3.12 -9.67 -0.10 -2.98 7.01 -2.97
Golgi -3.94 -0.51 -1.55 3.47 -1.49 -3.52 1.84 -0.82 3.23 -2.55 -5.00 1.78 -0.89 4.69 -3.00
Lipid Particles 2.10 4.05 4.73 9.11 3.26 -3.24 7.86 8.22 11.25 1.27 -1.95 8.36 9.09 14.61 2.61
Mitochondria -4.29 1.43 0.35 4.71 -4.19 -6.96 1.78 -2.11 6.39 -4.84 -7.88 2.12 -1.39 7.62 -5.15
Mitotic Spindle -4.85 -1.07 -5.81 -1.79 -0.74 -5.44 -1.84 -10.70 -5.51 -10.26 -6.71 -1.58 -11.77 -6.33 -8.83
None -3.48 3.79 0.83 4.53 -5.12 1.41 5.25 -0.90 -2.71 -7.29 -2.42 6.03 -0.11 2.63 -8.55
Nuclear Periphery -6.15 -4.90 -14.77 -9.54 -9.53 0.41 -4.02 -6.37 -6.94 -4.13 -1.86 -4.42 -9.19 -8.89 -6.41
Nuclear Periphery Foci -2.12 -3.29 -3.90 -1.77 1.28 -2.47 -1.57 -1.99 0.15 -0.61 -3.04 -3.34 -3.84 -0.85 -0.06
Nucleolus -3.35 1.07 2.23 9.54 3.78 -5.80 7.18 5.03 7.84 -1.11 -8.24 6.35 6.15 11.91 0.47
Nucleus -4.20 -9.41 -5.66 -2.94 6.92 9.88 -16.11 5.11 -5.62 21.15 7.45 -17.37 2.78 -5.44 21.74
Peroxisomes -2.52 4.28 4.31 5.88 0.57 -6.71 3.41 4.05 8.92 0.33 -6.53 3.86 4.44 10.60 0.39
Vacuole -4.30 -4.00 -16.03 -13.96 -3.11 -9.57 -7.62 -20.49 -12.34 -4.24 -11.64 -8.64 -26.34 -18.68 -6.01
Vacuole Periphery -4.12 0.56 -0.91 2.88 -1.20 -5.06 2.60 -3.20 1.42 -3.55 -6.75 2.10 -3.44 2.60 -3.82
Standard name
Human Ortholog
Description SUMO E3 ligase; catalyzes sumoylation of Yku70p/80p and Sir4p promoting telomere anchoring to the nuclear envelope and regulating telomerase activity; DNA-bound form catalyzes a DNA-damaged triggered sumoylation wave resulting in multisite modification of several DNA repair proteins, enhancing interactions between these proteins and accelerating repair; sumoylates Cse4p, a prerequisite for STUbL-mediated Ub-dependent degradation; role in telomere length maintenance
Localization
Cell Percentages nucleus (70%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Nfi1

Nfi1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Nfi1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available