Standard name
Human Ortholog
Description Protein involved in inserting tail-anchored proteins into ER membranes; forms a complex with Mdy2p; highly conserved across species and homologous to human gene C7orf20
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.07 0 0 0 0 0 0
Cytoplasm 0.97 0.91 0.96 0.97 0.97 0.89 0.79 0.76 0.63 0.64 0.51 0.54 0.99 0.99 0.99 0.91 0.92 0.96 0.97 0.81 0.94 0.95 0.92 0.87
Endoplasmic Reticulum 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.12 0.19 0.16 0.34 0.25 0.49 0.29 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0.1 0.12 0.1 0.17 0.2 0.16 0.19 0 0 0 0.12 0.09 0 0 0.11 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 4 3
Bud 1 0 0 0 1 1 6 4 8 5 6 7 0 1 0 0 0 0 0 0 0 0 3 5
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 2
Bud Site 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 4 0 1 5 5 6 6 5 5 2 8 6 4 3 6 17 17 0 0 0 0 0 0
Cytoplasm 144 90 119 253 401 316 390 317 245 269 223 239 519 619 711 214 229 246 144 100 127 177 503 475
Endoplasmic Reticulum 2 5 0 2 2 1 5 3 0 2 3 11 3 3 2 5 6 12 0 1 0 1 4 4
Endosome 0 0 0 1 0 1 4 1 1 2 0 1 1 1 3 3 0 0 0 2 0 0 3 9
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 2 2 0 1 0 0 1 1
Mitochondria 3 2 0 5 4 41 93 67 131 107 213 130 0 0 0 0 2 1 0 0 0 2 6 6
Nucleus 0 2 2 2 0 3 11 12 5 15 21 27 2 4 2 2 1 1 1 2 0 0 0 4
Nuclear Periphery 0 0 0 0 0 0 1 0 3 0 0 0 0 0 1 0 0 0 0 0 0 0 0 2
Nucleolus 0 0 0 0 0 0 0 2 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 2 8
Vac/Vac Membrane 3 0 4 5 14 34 60 40 67 82 68 86 2 4 11 28 22 6 1 13 4 1 6 18
Unique Cell Count 149 99 124 262 412 356 494 416 390 420 437 445 523 626 721 235 248 256 150 124 136 187 545 546
Labelled Cell Count 153 103 125 269 427 403 576 452 465 487 538 510 533 637 734 260 279 285 150 124 136 187 545 546


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.6 7.6 6.5 6.8 7.1 5.9 5.7 6.1 5.5 5.7 5.1 5.8 7.9 8.1 7.7 10.4 11.3 11.5 7.3 7.6 7.8
Std Deviation (1e-4) 0.8 1.3 1.0 1.2 1.3 1.0 1.3 1.3 1.3 1.1 0.9 1.0 1.0 1.1 1.2 2.1 2.4 2.6 1.2 1.3 1.3
Intensity Change (Log2) – – – 0.05 0.12 -0.15 -0.21 -0.09 -0.25 -0.19 -0.35 -0.18 0.27 0.3 0.23 0.67 0.78 0.81 0.16 0.23 0.25


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 3.0 2.9
Cytoplasm 0.3 0.8 -2.4 -4.4 -4.9 -7.1 -6.9 -9.1 -8.6 2.8 2.4 2.1 -1.7 -1.3 0.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 4.0 5.2 4.8 7.5 6.3 9.9 6.9 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 1.6 2.0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 2.2 2.9 2.3 3.9 4.4 3.6 4.3 0 0 0 2.7 2.0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.0526 3.1603 2.6143 1.8928 2.2666 2.7534 2.8935 4.0764 3.5587 3.4185 3.226 3.832 2.7307 4.2764 3.407 3.0091 2.9983 3.4971
Actin 0.0039 0.0053 0.0068 0.0018 0.0192 0.0004 0.0274 0.0001 0.0065 0.0063 0.0005 0.0023 0.0271 0.0003 0.0085 0.0007 0.022 0.0066
Bud 0.0004 0.0006 0.0004 0.0094 0.0006 0.0001 0.0004 0.0001 0.0009 0.0002 0.0002 0.0001 0.0021 0.0002 0.0006 0.0003 0.0011 0.001
Bud Neck 0.0087 0.0003 0.0018 0.0005 0.0011 0.0011 0.0061 0.0001 0.0037 0.0007 0.0006 0.0026 0.0044 0.0001 0.0022 0.0005 0.0014 0.001
Bud Periphery 0.0003 0.0003 0.0002 0.003 0.0006 0 0.0005 0 0.001 0.0001 0 0.0002 0.0021 0.0001 0.0003 0.0003 0.0007 0.0008
Bud Site 0.0018 0.0027 0.002 0.0005 0.0005 0.0001 0.002 0.0003 0.0041 0.0002 0.0002 0.0001 0.0034 0.0005 0.0045 0.0012 0.0018 0.0002
Cell Periphery 0.0001 0.0001 0.0001 0.0001 0.0001 0 0.0002 0 0.0001 0 0 0 0.0002 0 0.0001 0 0.0001 0
Cytoplasm 0.7589 0.913 0.8548 0.8635 0.8618 0.8853 0.7563 0.9525 0.8767 0.8993 0.9456 0.9317 0.8291 0.9407 0.9013 0.9096 0.8209 0.9231
Cytoplasmic Foci 0.0312 0.0129 0.0152 0.0169 0.0134 0.017 0.0181 0.0046 0.013 0.0079 0.0199 0.0085 0.017 0.0099 0.0158 0.0134 0.037 0.0119
Eisosomes 0 0 0 0 0 0 0.0004 0 0.0002 0 0 0 0.0002 0 0.0002 0 0.0001 0
Endoplasmic Reticulum 0.005 0.002 0.0026 0.0028 0.0043 0.0022 0.0093 0.0014 0.0025 0.0053 0.0021 0.0038 0.0044 0.0022 0.0042 0.005 0.0038 0.0022
Endosome 0.0312 0.0058 0.0107 0.0128 0.0232 0.012 0.0294 0.0023 0.0073 0.01 0.0097 0.0082 0.0201 0.0114 0.0103 0.013 0.0341 0.0066
Golgi 0.0036 0.0009 0.0011 0.0007 0.0103 0.0003 0.0071 0.0001 0.0015 0.0014 0.0004 0.0021 0.0055 0.0009 0.0029 0.0035 0.0089 0.0029
Lipid Particles 0.0022 0.0001 0.0011 0.0001 0.0019 0 0.0193 0 0.0013 0.0019 0 0.0015 0.0034 0.0001 0.0025 0.0102 0.016 0.0019
Mitochondria 0.0012 0.0004 0.001 0.0011 0.0066 0.0004 0.0068 0.0001 0.0013 0.0005 0.0003 0.0054 0.0047 0.0004 0.0043 0.0022 0.0022 0.0021
None 0.1207 0.0477 0.0865 0.0523 0.0433 0.0702 0.0773 0.0347 0.0639 0.0475 0.0119 0.0265 0.0593 0.0283 0.0285 0.0214 0.0162 0.0308
Nuclear Periphery 0.0047 0.001 0.002 0.0021 0.0036 0.0011 0.0093 0.0005 0.0018 0.0042 0.0008 0.0008 0.0022 0.001 0.0015 0.0014 0.0024 0.0015
Nucleolus 0.0005 0.0001 0.0001 0.0004 0.0001 0.0001 0.0009 0 0.0004 0.0002 0 0.0001 0.0003 0 0.0002 0.0003 0.0004 0.0001
Nucleus 0.0106 0.0039 0.0089 0.0248 0.0045 0.0058 0.0051 0.0019 0.0046 0.007 0.0043 0.003 0.0051 0.0017 0.0041 0.0027 0.0043 0.0037
Peroxisomes 0.0037 0.0004 0.001 0.0004 0.0012 0.0003 0.0127 0 0.0045 0.0006 0.0012 0.0006 0.0042 0.0003 0.0041 0.0107 0.0155 0.0014
Punctate Nuclear 0.0067 0.0008 0.0019 0.0024 0.0015 0.0021 0.0082 0.0004 0.0031 0.0051 0.0009 0.0011 0.0016 0.0003 0.0023 0.001 0.0081 0.001
Vacuole 0.0036 0.0015 0.0015 0.0039 0.0018 0.0012 0.002 0.0008 0.0011 0.001 0.001 0.0009 0.0028 0.0013 0.0011 0.002 0.0023 0.0008
Vacuole Periphery 0.0007 0.0002 0.0004 0.0006 0.0007 0.0003 0.0011 0.0001 0.0003 0.0003 0.0003 0.0004 0.0007 0.0004 0.0003 0.0004 0.0007 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 64.0421 60.9263 56.9022 66.6681 63.989 50.5007 63.7268 70.6102 67.7288 66.784
Translational Efficiency 1.6751 1.8555 1.6674 1.3443 1.418 2.0817 1.8056 1.4413 1.3828 1.7133

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1111 667 2944 301 1844 852 264 1121 2955 1519 3208 1422

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 832.20 832.91 1082.74 971.81 809.59 933.80 1195.53 1104.42 818.09 889.50 1092.02 1076.35
Standard Deviation 115.96 174.42 139.98 142.45 103.45 123.01 135.37 163.74 108.87 156.05 143.00 168.42
Intensity Change Log 2 — 0.001230 0.379685 0.223744 — 0.205922 0.562387 0.448026 — 0.105796 0.472669 0.338697

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000192 0.000673 0.000194 0.000365 0.000334 0.000658 0.000541 0.002654 0.000281 0.000664 0.000222 0.002169
Bud Neck 0.004721 0.004683 0.001757 0.003055 0.003919 0.008377 0.001851 0.001343 0.004220 0.006755 0.001765 0.001706
Bud Site 0.005489 0.021504 0.010247 0.012802 0.005671 0.018040 0.011439 0.018999 0.005603 0.019561 0.010345 0.017687
Cell Periphery 0.000128 0.000249 0.000043 0.000130 0.000158 0.000105 0.000320 0.000135 0.000147 0.000168 0.000065 0.000134
Cytoplasm 0.597613* 0.570507* 0.854012* 0.529710* 0.598635* 0.550576* 0.575559* 0.749047* 0.598251* 0.559328* 0.831097* 0.702619*
Cytoplasmic Foci 0.209052* 0.202485* 0.027590 0.084788 0.203076* 0.204367* 0.083871 0.074929 0.205322* 0.203541* 0.032222 0.077016
Eisosomes 0.000084 0.000069 0.000006 0.000048 0.000094 0.000061 0.000062 0.000019 0.000090 0.000065 0.000011 0.000025
Endoplasmic Reticulum 0.002754 0.002456 0.000231 0.003102 0.006142 0.002048 0.003667 0.000897 0.004868 0.002227 0.000513 0.001363
Endosome 0.010860 0.026568 0.001084 0.013408 0.011295 0.022718 0.014168 0.003967 0.011131 0.024408 0.002160 0.005966
Golgi 0.003219 0.003290 0.000526 0.003445 0.004275 0.007062 0.000408 0.000792 0.003878 0.005406 0.000516 0.001354
Lipid Particles 0.005773 0.006023 0.000553 0.002304 0.006688 0.003412 0.003495 0.002037 0.006344 0.004559 0.000795 0.002093
Mitochondria 0.002993 0.002126 0.000257 0.000245 0.002253 0.003289 0.000058 0.000097 0.002531 0.002779 0.000241 0.000129
Mitotic Spindle 0.001351 0.001541 0.002909 0.005881 0.001266 0.009534 0.000743 0.008089 0.001298 0.006024 0.002731 0.007622
None 0.001841 0.007838 0.002326 0.001833 0.002556 0.001331 0.001108 0.001059 0.002287 0.004188 0.002225 0.001223
Nuclear Periphery 0.000344 0.000370 0.000113 0.000867 0.000272 0.000323 0.000581 0.000145 0.000299 0.000343 0.000151 0.000298
Nuclear Periphery Foci 0.002118 0.004530 0.001093 0.007471 0.001630 0.001265 0.017834 0.005857 0.001813 0.002699 0.002471 0.006198
Nucleolus 0.000533 0.003833 0.000131 0.000366 0.000440 0.001407 0.000262 0.000095 0.000475 0.002472 0.000142 0.000152
Nucleus 0.075642 0.044873 0.059627 0.081010 0.079598 0.045915 0.047066 0.033214 0.078111 0.045457 0.058593 0.043331
Peroxisomes 0.002596 0.003292 0.000633 0.001839 0.001633 0.006674 0.000464 0.001232 0.001995 0.005189 0.000619 0.001361
Vacuole 0.071639 0.090391 0.036557 0.245997* 0.068351 0.109643 0.236133* 0.095195 0.069587 0.101189 0.052981 0.127116
Vacuole Periphery 0.001059 0.002698 0.000113 0.001335 0.001715 0.003197 0.000370 0.000198 0.001468 0.002978 0.000134 0.000439

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.74 -1.09 -2.59 1.66 -1.19 -1.46 -1.83 -1.72 -1.30 -1.48 -2.63 -0.03 -1.83 -1.19 -1.82
Bud Neck 0.13 4.78 2.96 2.99 -2.31 -4.74 6.83 10.52 7.51 2.99 -4.22 6.21 9.84 8.82 0.59
Bud Site -7.71 -3.72 -2.88 2.98 -1.26 -5.61 -5.40 -7.78 0.79 -2.73 -9.07 -4.16 -8.38 2.28 -4.29
Cell Periphery -2.94 11.78 0.35 3.00 -8.07 5.51 -3.80 3.73 -1.86 4.93 -1.02 13.72 3.22 2.33 -8.70
Cytoplasm 2.17 -27.95 5.67 3.69 20.88 4.69 3.56 -11.47 -12.88 -8.96 4.97 -35.67 -7.53 -10.45 18.62
Cytoplasmic Foci 0.67 29.82 16.01 13.47 -9.05 0.08 19.13 25.78 18.56 0.70 0.47 46.98 30.91 23.78 -13.88
Eisosomes 1.09 11.46 4.92 1.66 -12.55 4.56 4.88 16.29 7.16 8.88 3.53 20.66 16.61 6.11 -10.89
Endoplasmic Reticulum 0.68 8.22 -0.24 -0.83 -6.63 3.94 2.89 6.54 1.86 5.57 4.06 8.30 6.81 2.34 -4.98
Endosome -7.04 14.00 -0.44 6.11 -8.29 -5.96 -0.84 11.82 10.80 8.46 -9.12 18.44 10.56 13.79 -7.68
Golgi -0.05 6.36 0.41 0.43 -0.95 -1.65 12.55 11.21 4.20 -1.38 -1.53 11.82 5.91 4.52 -1.06
Lipid Particles -0.15 9.40 5.70 2.60 -7.30 5.62 6.35 8.87 2.41 2.88 2.54 15.88 10.30 3.36 -6.86
Mitochondria 0.49 2.32 2.35 1.29 0.56 -0.89 3.40 3.37 3.81 -0.59 -0.17 3.84 4.04 3.31 2.43
Mitotic Spindle -0.18 -1.91 -1.68 -1.64 -1.01 -3.88 -0.27 -3.27 0.94 -3.06 -3.79 -2.46 -3.62 -0.30 -2.61
None -3.41 -0.55 0.69 3.54 1.05 2.24 4.67 5.72 0.71 0.11 -2.28 0.82 5.45 3.76 2.60
Nuclear Periphery -0.22 3.25 -4.00 -3.46 -5.78 -1.84 -7.71 2.20 3.34 9.04 -1.24 3.11 -1.53 0.07 -4.55
Nuclear Periphery Foci -4.22 2.16 -5.89 -3.19 -7.16 1.05 -10.84 -8.91 -9.27 7.85 -3.45 -3.76 -10.25 -7.40 -8.12
Nucleolus -2.98 8.14 2.95 3.16 -4.14 -2.16 4.90 14.28 2.86 3.56 -3.66 13.13 12.74 4.24 -0.03
Nucleus 6.57 2.55 -0.05 -4.30 -1.37 9.19 5.37 13.30 1.83 2.04 11.24 5.25 11.01 -0.80 7.23
Peroxisomes -0.81 2.75 1.03 1.96 -1.04 -4.43 5.66 1.82 4.92 -2.33 -4.42 4.52 2.16 5.75 -1.98
Vacuole -3.70 4.92 -15.72 -13.72 -17.21 -8.48 -13.59 -13.39 -4.01 8.38 -9.04 -1.67 -18.97 -10.95 -17.51
Vacuole Periphery -3.42 11.06 0.24 2.87 -2.36 -2.30 7.04 7.89 5.21 5.18 -3.60 10.52 7.64 6.72 -2.52
Standard name
Human Ortholog
Description Protein involved in inserting tail-anchored proteins into ER membranes; forms a complex with Mdy2p; highly conserved across species and homologous to human gene C7orf20
Localization
Cell Percentages cytoplasm (94%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Get4

Get4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Get4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available