Standard name
Human Ortholog
Description Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.08 0 0.08 0.06 0.06 0.07 0 0.05 0 0 0.06 0.13 0.12 0.17 0.11 0.06 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.19 0.33 0 0.05 0 0.14 0.11 0.17 0.52 0.42 0.37 0.36 0 0 0 0.1 0.1 0.07 0 0 0 0 0 0
Nucleus 0.84 0.8 0.84 0.9 0.84 0.81 0.83 0.77 0.68 0.72 0.73 0.68 0.83 0.81 0.79 0.75 0.77 0.75 0.75 0.78 0.65 0.71 0.68 0.56
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.35 0.12 0 0.07 0 0.07 0.05 0.09 0.14 0.13 0.21 0.23 0.08 0.07 0.07 0.07 0.11 0.14 0.12 0.09 0.17 0.12 0.14 0.18
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.05 0.05 0 0.06 0.08
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 2 0 0 0
Bud 0 1 0 0 0 2 2 6 5 3 3 9 0 2 0 1 1 2 0 1 3 2 1 1
Bud Neck 0 0 1 0 0 1 0 0 0 3 2 2 0 0 0 0 1 0 0 0 1 0 0 0
Bud Site 0 0 0 0 0 7 4 5 14 12 5 26 0 0 0 1 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 5 31 8 19 27 35 42 12 22 8 10 8 37 38 25 15 9 0 1 8 5 4 5
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 4 3 1 0 0 1 0 0 0
Endosome 0 1 11 3 3 3 8 7 1 1 0 2 5 1 2 2 3 4 2 2 9 2 5 6
Golgi 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 1 1 0
Mitochondria 7 62 5 11 7 61 61 99 245 196 191 191 1 5 2 14 13 10 0 3 6 2 1 6
Nucleus 31 149 326 213 206 357 448 450 321 336 376 367 121 238 242 110 101 112 53 159 285 138 155 124
Nuclear Periphery 0 0 2 2 3 7 8 5 2 4 1 8 1 2 5 5 0 4 0 0 2 1 1 2
Nucleolus 13 22 14 17 7 33 28 53 65 59 106 125 12 20 22 10 14 21 8 17 74 23 32 40
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 1 21 3 9 7 14 21 4 7 7 16 3 6 9 5 3 5 0 3 9 4 5 8
Vac/Vac Membrane 0 7 9 4 9 8 4 14 5 8 3 4 0 6 2 2 5 7 2 9 23 8 12 17
Unique Cell Count 37 187 389 236 244 443 543 585 472 469 513 537 145 293 305 146 132 150 72 204 439 195 229 221
Labelled Cell Count 51 248 422 261 263 513 613 702 675 651 702 760 152 317 322 179 159 175 72 204 439 195 229 221


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.1 4.9 7.3 6.6 7.4 5.8 5.8 5.9 4.6 4.7 4.9 5.0 6.6 6.5 6.7 6.4 7.5 6.7 6.9 7.3 7.1
Std Deviation (1e-4) 1.1 0.9 2.1 1.9 2.7 1.7 1.9 1.8 1.4 1.4 1.0 1.1 1.3 1.4 1.6 2.0 2.2 1.7 1.7 2.1 2.3
Intensity Change (Log2) – – – -0.14 0.02 -0.34 -0.34 -0.3 -0.66 -0.65 -0.56 -0.54 -0.13 -0.17 -0.12 -0.19 0.04 -0.12 -0.08 0.01 -0.03


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.3433 5.1199 5.3687 4.5183 4.9055 5.3124 6.1616 5.6556 5.1053 3.916 4.9222 4.5469 2.7808 3.0221 2.3964 2.1364 2.7731 2.7004
Actin 0.0026 0.0002 0.0067 0.0013 0.0059 0.0154 0.0323 0 0.0162 0.0001 0.0685 0 0.0394 0.0001 0.0308 0.0434 0.0433 0.0101
Bud 0.0004 0.0001 0.0004 0.0001 0.0003 0.0007 0.0006 0 0.0012 0.0001 0.0007 0 0.0007 0 0.0003 0.0001 0.0008 0.0002
Bud Neck 0.0017 0.0002 0.0024 0.0002 0.0015 0.0024 0.0017 0.0001 0.0043 0.0005 0.0011 0.0014 0.0013 0.0002 0.0017 0.0003 0.0013 0.0023
Bud Periphery 0.0011 0.0001 0.001 0.0001 0.0004 0.0015 0.0008 0 0.0018 0.0001 0.0007 0.0001 0.0017 0 0.0005 0.0001 0.0012 0.0003
Bud Site 0.0013 0.0008 0.0047 0.0002 0.0033 0.0009 0.0014 0.0001 0.0065 0.0002 0.0014 0.0001 0.0018 0.0002 0.0032 0.0004 0.0058 0.0002
Cell Periphery 0.0002 0 0.0003 0 0.0003 0.0001 0.0003 0 0.0003 0 0.0001 0 0.0004 0 0.0001 0 0.0003 0
Cytoplasm 0.0124 0.0001 0.0071 0.0001 0.013 0.0012 0.0637 0.0002 0.0147 0.0003 0.0014 0.0001 0.0314 0.0018 0.0011 0.0001 0.008 0.0008
Cytoplasmic Foci 0.0101 0.0023 0.0039 0.0023 0.0054 0.0031 0.019 0 0.016 0.0001 0.0107 0 0.0161 0.0002 0.0068 0.0043 0.0128 0.0025
Eisosomes 0.0001 0 0.0002 0 0.0001 0.0001 0.0002 0 0.0001 0 0.0001 0 0.0005 0 0.0002 0.0001 0.0003 0.0001
Endoplasmic Reticulum 0.004 0 0.0022 0.0002 0.0131 0.004 0.0155 0 0.0077 0 0.0008 0 0.0072 0 0.0005 0 0.0036 0.0014
Endosome 0.0145 0.0024 0.0141 0.0117 0.0251 0.0112 0.0908 0 0.0298 0.0002 0.0182 0 0.0207 0.0001 0.0038 0.0009 0.06 0.0054
Golgi 0.0122 0.0014 0.0025 0.009 0.0027 0.0075 0.0153 0 0.0116 0.0001 0.0182 0 0.005 0 0.0027 0.0018 0.0107 0.0017
Lipid Particles 0.0133 0.0042 0.0034 0.0229 0.0078 0.0088 0.0497 0 0.0129 0.0001 0.0044 0 0.0103 0 0.007 0.019 0.0115 0.0056
Mitochondria 0.0028 0.0024 0.0131 0.0058 0.0034 0.011 0.0048 0.0005 0.0046 0.0002 0.0038 0.0001 0.0251 0.0001 0.001 0.0011 0.0041 0.0022
None 0.007 0.0001 0.0044 0.0002 0.0051 0.0004 0.019 0.0002 0.0017 0.0004 0.0011 0 0.0189 0.0011 0.0011 0.0001 0.017 0.0024
Nuclear Periphery 0.0102 0.0007 0.015 0.0034 0.0364 0.0075 0.1284 0.003 0.0271 0.0033 0.0019 0.0003 0.0194 0.0064 0.003 0.0008 0.012 0.0092
Nucleolus 0.0445 0.0402 0.0382 0.0235 0.1461 0.1168 0.0509 0.0316 0.0474 0.0775 0.0951 0.0817 0.0341 0.0544 0.0564 0.0391 0.0626 0.0859
Nucleus 0.8037 0.9247 0.8539 0.9091 0.6304 0.7833 0.4631 0.9632 0.751 0.8746 0.7555 0.8987 0.7293 0.8984 0.8344 0.8571 0.7129 0.8495
Peroxisomes 0.0137 0.0022 0.0014 0.0019 0.0009 0.0071 0.005 0 0.0191 0 0.01 0 0.0158 0 0.0125 0.0153 0.0065 0.0029
Punctate Nuclear 0.0416 0.0176 0.0197 0.0066 0.0944 0.016 0.0263 0.0008 0.0207 0.042 0.0055 0.0172 0.0148 0.0367 0.0322 0.0157 0.0143 0.0164
Vacuole 0.0019 0.0002 0.0039 0.0005 0.0031 0.0005 0.0053 0.0001 0.0038 0.0001 0.0004 0.0001 0.004 0.0001 0.0007 0.0001 0.0087 0.0003
Vacuole Periphery 0.0007 0.0001 0.0017 0.0009 0.0013 0.0005 0.006 0.0001 0.0016 0.0001 0.0004 0 0.0023 0.0001 0.0002 0.0001 0.0023 0.0006

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 25.1654 9.2293 14.5531 17.1595 12.5492 25.7963 18.6634 17.7139 19.7258 19.76
Translational Efficiency 0.9956 1.8779 0.9927 1.1617 1.4372 0.7469 0.9272 0.9459 0.8123 0.8752

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
158 1331 2661 115 687 1301 2287 130 845 2632 4948 245

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 802.51 760.21 920.54 969.47 727.48 873.73 912.99 902.06 741.51 816.32 917.05 933.70
Standard Deviation 135.33 135.40 119.38 134.71 104.24 122.86 121.43 114.43 114.52 141.25 120.39 128.83
Intensity Change Log 2 — -0.078121 0.197961 0.272677 — 0.264280 0.327691 0.310316 — 0.094833 0.261102 0.290696

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001346 0.000863 0.000748 0.002682 0.000636 0.000467 0.000920 0.002872 0.000769 0.000667 0.000828 0.002783
Bud Neck 0.007008 0.013137 0.028401 0.011544 0.006110 0.009238 0.036000 0.021836 0.006278 0.011209 0.031914 0.017005
Bud Site 0.001597 0.002415 0.003888 0.025210 0.000720 0.001049 0.003210 0.016231 0.000884 0.001740 0.003575 0.020445
Cell Periphery 0.000117 0.000123 0.000075 0.000287 0.000049 0.000076 0.000067 0.000201 0.000062 0.000100 0.000071 0.000242
Cytoplasm 0.007222 0.014346 0.003817 0.029908 0.002497 0.001951 0.003079 0.058705 0.003380 0.008219 0.003476 0.045188
Cytoplasmic Foci 0.001377 0.001292 0.000052 0.025814 0.000191 0.001172 0.000108 0.003217 0.000413 0.001233 0.000078 0.013824
Eisosomes 0.000043 0.000028 0.000029 0.000070 0.000023 0.000024 0.000029 0.000069 0.000027 0.000026 0.000029 0.000069
Endoplasmic Reticulum 0.003681 0.003332 0.003024 0.011662 0.002246 0.002012 0.004011 0.008330 0.002514 0.002679 0.003481 0.009894
Endosome 0.000686 0.000887 0.000302 0.029798 0.000229 0.000736 0.000427 0.006051 0.000314 0.000813 0.000359 0.017198
Golgi 0.000676 0.000651 0.000140 0.039340 0.000161 0.000738 0.000164 0.023115 0.000258 0.000694 0.000151 0.030731
Lipid Particles 0.001857 0.001164 0.000077 0.001829 0.000221 0.001095 0.000147 0.001100 0.000527 0.001130 0.000110 0.001443
Mitochondria 0.004158 0.002476 0.001940 0.004407 0.001137 0.006272 0.002816 0.005461 0.001702 0.004353 0.002345 0.004966
Mitotic Spindle 0.035822 0.009514 0.006022 0.120465* 0.004433 0.011350 0.005301 0.131958* 0.010302 0.010421 0.005689 0.126563*
None 0.012859 0.020672 0.003810 0.011801 0.007647 0.006360 0.003389 0.022796 0.008622 0.013598 0.003616 0.017635
Nuclear Periphery 0.000861 0.001025 0.000657 0.002588 0.000469 0.001027 0.001399 0.001450 0.000543 0.001026 0.001000 0.001984
Nuclear Periphery Foci 0.001790 0.002313 0.000143 0.001502 0.000789 0.000665 0.001032 0.001774 0.000976 0.001498 0.000554 0.001646
Nucleolus 0.102536 0.086224 0.033216 0.009299 0.092939 0.084453 0.042419 0.031856 0.094734 0.085348 0.037470 0.021268
Nucleus 0.810675* 0.837207* 0.912077* 0.590397* 0.878654* 0.868331* 0.893201* 0.622191* 0.865943* 0.852592* 0.903353* 0.607267*
Peroxisomes 0.004316 0.001183 0.000206 0.000652 0.000466 0.001552 0.000275 0.003667 0.001186 0.001365 0.000238 0.002252
Vacuole 0.001037 0.000710 0.001163 0.079681 0.000224 0.000985 0.001415 0.036382 0.000376 0.000846 0.001280 0.056706
Vacuole Periphery 0.000337 0.000438 0.000213 0.001065 0.000157 0.000449 0.000588 0.000739 0.000191 0.000443 0.000387 0.000892

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 2.27 2.04 -3.72 -8.35 -8.11 1.25 -4.20 -6.67 -7.27 -5.61 1.19 -1.96 -9.20 -10.56 -9.13
Bud Neck -2.54 -13.19 -3.45 -2.02 5.89 -3.33 -22.80 -5.18 -4.26 5.66 -5.94 -28.62 -6.88 -4.58 7.65
Bud Site 0.51 -1.06 -3.44 -3.55 -3.26 -1.11 -5.59 -5.40 -5.31 -4.64 -2.03 -6.30 -6.52 -6.28 -5.61
Cell Periphery 0.22 2.26 -2.09 -2.33 -3.20 -0.70 -1.74 -7.22 -4.77 -6.92 -1.82 -0.35 -5.79 -4.60 -5.88
Cytoplasm -1.49 1.04 -3.07 -2.50 -4.34 0.26 -0.53 -4.81 -4.86 -4.78 -3.35 0.21 -6.11 -5.36 -6.24
Cytoplasmic Foci -0.39 1.86 -1.43 -1.33 -2.02 -2.70 2.32 -3.85 -1.93 -4.14 -3.09 2.82 -2.97 -1.98 -3.34
Eisosomes 3.82 3.32 -4.47 -7.48 -7.19 0.09 -4.54 -8.31 -8.34 -7.68 1.90 -1.00 -10.53 -11.21 -10.54
Endoplasmic Reticulum 2.70 2.66 -6.24 -8.30 -8.30 0.66 -7.59 -7.25 -7.41 -5.98 0.45 -4.08 -10.59 -10.89 -9.85
Endosome -0.19 3.02 -1.92 -1.89 -2.14 -3.09 -2.34 -4.85 -4.40 -4.71 -3.34 0.25 -3.97 -3.62 -3.98
Golgi -0.14 1.86 -0.98 -0.97 -1.10 -2.25 -1.46 -1.12 -1.03 -1.11 -2.97 0.98 -1.49 -1.37 -1.50
Lipid Particles 0.46 1.64 -0.50 -1.77 -6.38 -1.29 1.49 -6.33 -1.96 -6.77 -1.38 2.19 -5.94 -2.65 -9.32
Mitochondria 0.77 0.90 -0.09 -2.16 -2.54 -3.38 -8.60 -2.87 1.73 -0.04 -3.41 -3.54 -3.45 0.88 -1.60
Mitotic Spindle 2.72 2.85 -1.91 -4.08 -4.16 -3.08 -2.11 -5.89 -5.49 -5.71 0.11 1.14 -6.72 -6.82 -7.04
None -2.11 1.80 1.37 6.16 -0.94 0.72 2.29 -1.84 -2.43 -3.37 -2.44 2.75 -0.78 1.32 -3.43
Nuclear Periphery 1.14 1.72 -6.36 -7.64 -8.13 -2.81 -6.70 -7.32 -4.07 -3.22 -2.97 -5.61 -10.65 -8.28 -8.33
Nuclear Periphery Foci 0.45 3.37 -0.74 -1.14 -3.33 0.71 0.78 -2.55 -2.69 -2.70 -0.29 2.50 -2.93 -2.87 -3.60
Nucleolus 0.63 4.34 7.46 22.12* 15.82 1.53 8.24 9.73 9.90 4.51 1.63 9.92 14.27 18.79 9.44
Nucleus -1.72 -4.55 3.72 5.66 7.67 1.26 0.28 9.18 8.90 9.26 1.50 -3.22 10.56 10.34 12.03
Peroxisomes 0.98 1.41 1.16 1.11 -2.89 -2.75 2.76 -2.09 -0.69 -2.36 -0.68 1.82 -0.79 -0.30 -2.91
Vacuole 2.67 2.13 -5.93 -6.13 -6.10 -0.52 -1.75 -5.13 -5.11 -5.03 0.63 -0.58 -7.90 -7.95 -7.87
Vacuole Periphery -0.79 1.53 -2.00 -1.51 -4.58 -2.09 -1.55 -4.73 -1.66 -1.62 -3.15 -1.36 -6.05 -2.22 -3.47
Standard name
Human Ortholog
Description Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation
Localization
Cell Percentages nucleus (97%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Med4

Med4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Med4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available