Standard name
Human Ortholog
Description Actin assembly factor; C-terminal WCA domain activates Arp2/3 complex-mediated nucleation of branched actin filaments, polyproline domain nucleates actin filaments independent of Arp2/3; mutants are defective in endocytosis, bud site selection, cytokinesis; human homolog WAS (Wiskott-Aldrich Syndrome) implicated in severe immunodeficiency; human WAS complements yeast null mutant, but only in presence of WIPF1, which mediates localization of WAS to cortical patches
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.68 0.72 – – 0.68 0.49 0.61 0.62 0.4 0.41 0.35 0.4 0.7 0.74 0.61 0.51 0.52 0.44 0.78 0.69 0.56 0.69 0.67 0.7
Bud 0.18 0.18 – – 0.32 0.16 0.15 0.21 0.17 0.14 0.15 0.15 0.14 0.15 0.13 0.16 0.06 0.09 0.05 0.1 0.06 0.06 0.06 0.05
Bud Neck 0 0 – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0.09 0.08 0.08
Bud Site 0.12 0.09 – – 0 0.12 0 0 0 0.06 0 0 0.09 0 0 0 0 0 – – – – – –
Cell Periphery 0.09 0.17 – – 0.05 0 0.07 0.13 0.08 0.1 0 0 0.15 0.18 0.23 0.11 0.15 0.09 0 0 0 0 0 0
Cytoplasm 0 0.09 – – 0.11 0.07 0.07 0.07 0 0 0 0.06 0.14 0.07 0.1 0.13 0.1 0.12 0 0 0 0 0 0
Endoplasmic Reticulum 0 0.07 – – 0.05 0.12 0.13 0.17 0.05 0.11 0.09 0 0.09 0.1 0.07 0 0 0 0 0 0 0 0 0
Endosome 0 0 – – 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0.05 – – 0.16 0.07 0.09 0.09 0.1 0.12 0 0.11 0 0.09 0.08 0.18 0.29 0.2 0 0 0 0 0 0
Mitochondria 0.49 0.29 – – 0 0.32 0.26 0.22 0.48 0.52 0.8 0.63 0.23 0.05 0 0.13 0.1 0.07 0 0 0.05 0 0 0
Nucleus 0 0 – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 – – 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.36 0.23 – – 0.16 0.29 0.22 0.18 0.24 0.22 0.14 0.16 0.2 0.34 0.34 0.24 0.38 0.22 0.05 0 0.1 0.05 0.05 0
SpindlePole 0 0 – – 0.05 0 0 0.07 0.05 0 0 0 0 0 0.05 0.09 0.1 0.18 0 0 0 0 0 0
Vac/Vac Membrane 0 0 – – 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 124 101 – – 13 51 84 92 57 43 39 53 57 153 127 23 25 40 158 116 22 99 170 180
Bud 32 25 – – 6 17 21 31 24 15 16 20 11 32 28 7 3 8 10 16 2 8 16 13
Bud Neck 0 0 – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 8 13 0 13 20 19
Bud Site 21 13 – – 0 12 5 6 5 6 4 5 7 7 2 1 1 2 – – – – – –
Cell Periphery 16 24 – – 1 4 10 19 12 11 4 5 12 37 48 5 7 8 3 2 0 0 2 5
Cytoplasm 7 12 – – 2 7 10 10 3 3 4 8 11 14 21 6 5 11 1 0 1 2 2 4
Endoplasmic Reticulum 5 10 – – 1 13 18 25 7 12 10 4 7 20 15 1 1 4 0 0 1 0 2 1
Endosome 1 0 – – 1 1 1 4 3 2 0 1 0 2 1 1 0 3 5 6 1 1 7 8
Golgi 4 7 – – 3 7 13 14 14 13 4 15 1 19 17 8 14 18 0 1 1 0 2 1
Mitochondria 89 41 – – 0 33 36 33 68 55 88 82 19 10 7 6 5 6 3 1 1 3 7 4
Nucleus 0 1 – – 0 1 1 1 0 1 1 1 0 1 2 1 0 1 0 1 0 0 0 0
Nuclear Periphery 2 0 – – 0 0 0 0 2 1 2 0 0 0 0 0 1 0 0 0 1 0 0 0
Nucleolus 2 0 – – 1 1 1 0 0 0 2 1 1 0 0 1 0 2 0 0 0 0 0 0
Peroxisomes 66 32 – – 3 30 30 27 34 23 15 21 16 71 70 11 18 20 9 4 4 6 12 10
SpindlePole 3 2 – – 1 3 4 10 7 3 1 5 2 9 10 4 5 16 0 0 1 0 0 0
Vac/Vac Membrane 1 0 – – 1 3 3 3 4 4 0 1 0 1 0 0 1 4 1 0 0 2 6 2
Unique Cell Count 182 140 19 104 137 149 142 106 110 131 81 207 208 45 48 90 204 169 40 143 256 257
Labelled Cell Count 373 268 33 183 237 275 240 192 190 222 144 376 348 75 86 143 204 169 40 143 256 257


Actin

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.4 8.8 – – 10.1 8.7 8.9 8.8 7.4 7.9 6.9 7.7 8.3 8.5 8.5 10.7 9.8 10.6 8.0 8.3 8.5
Std Deviation (1e-4) 1.4 2.2 – – 2.0 2.6 2.3 1.9 1.8 2.1 1.8 2.1 1.7 1.7 1.7 3.0 2.5 3.4 1.5 1.4 1.4
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.3038 3.5225 2.8834 3.3591 2.2393 3.3846 3.3876 4.0146 2.946 2.4512 2.8675 3.2896 4.9502 3.8018 3.9478 4.3135 3.7675 3.8978
Actin 0.7204 0.6878 0.8833 0.742 0.6041 0.8814 0.784 0.5966 0.7431 0.7687 0.8884 0.8021 0.873 0.6901 0.8991 0.9561 0.9368 0.8843
Bud 0.0007 0.0014 0.0063 0.0067 0.0097 0.0015 0.001 0.0026 0.0159 0.0131 0.0022 0.0018 0.0028 0.0056 0.0087 0.0043 0.0022 0.0011
Bud Neck 0.022 0.0099 0.0125 0.0383 0.004 0.0546 0.0117 0.0115 0.0341 0.0039 0.0008 0.1104 0.0122 0.0097 0.0184 0.0063 0.0459 0.0844
Bud Periphery 0.001 0.0016 0.0128 0.0223 0.0275 0.0071 0.0015 0.0044 0.0189 0.0377 0.005 0.0133 0.0086 0.0056 0.0194 0.0111 0.0072 0.0054
Bud Site 0.0328 0.276 0.0401 0.0034 0.0085 0.002 0.0362 0.3305 0.0944 0.0119 0.0011 0.0039 0.0365 0.2319 0.0417 0.0061 0.0023 0.0023
Cell Periphery 0.0027 0.0033 0.002 0.0006 0.0028 0.0008 0.0015 0.0017 0.0012 0.0008 0.0001 0.0016 0.002 0.0012 0.0007 0.0013 0.0005 0.0007
Cytoplasm 0.0025 0.0117 0.0038 0.0023 0.0681 0.0005 0.0038 0.0191 0.0023 0.0008 0.0008 0.0007 0.0012 0.0174 0.0082 0.0002 0.0001 0.0038
Cytoplasmic Foci 0.0328 0.0019 0.0074 0.0344 0.0189 0.0055 0.028 0.0057 0.0095 0.0219 0.0169 0.0076 0.0086 0.0126 0.0004 0.0002 0.0008 0.0014
Eisosomes 0.0036 0.0021 0.0033 0.0013 0.0006 0.0037 0.0139 0.0026 0.002 0.0013 0.0006 0.0079 0.0283 0.0021 0.0015 0.0133 0.0017 0.0091
Endoplasmic Reticulum 0.0068 0.0001 0.0003 0.0012 0.0007 0.0021 0.0014 0.0003 0.0003 0.0022 0.0002 0.0001 0.0007 0.0001 0.0001 0 0 0.0001
Endosome 0.0242 0.0001 0.0011 0.0229 0.0278 0.0098 0.0294 0.0026 0.0177 0.0119 0.0282 0.0013 0.0033 0.0008 0.0001 0 0.0001 0.0008
Golgi 0.0159 0.0006 0.0024 0.0236 0.0403 0.0066 0.0143 0.003 0.0129 0.0123 0.0026 0.0036 0.0033 0.0031 0.0003 0.0005 0.0007 0.0022
Lipid Particles 0.0508 0.0002 0.0057 0.0216 0.0251 0.0017 0.0271 0.0003 0.0126 0.0372 0.0213 0.0025 0.0049 0.0006 0 0 0.0002 0.0002
Mitochondria 0.0221 0.0002 0.0019 0.0159 0.1331 0.0024 0.0167 0.0004 0.0188 0.0133 0.0003 0.0281 0.0012 0.0006 0.0004 0.0001 0.0001 0.0004
None 0.0158 0.0001 0.0046 0.0054 0.0004 0.0003 0.0023 0.0002 0.0008 0.002 0.0005 0.0003 0.0015 0.0125 0.0002 0 0 0.0001
Nuclear Periphery 0.0031 0 0.0002 0.0071 0.0004 0.0043 0.001 0.0002 0.0002 0.0037 0.0032 0 0.0005 0 0 0 0 0
Nucleolus 0.0013 0 0.0002 0.0029 0.0002 0.0001 0.0004 0 0.0001 0.0006 0.0006 0.0001 0.0002 0 0 0 0 0
Nucleus 0.0009 0 0.0001 0.0038 0.0003 0.0003 0.0004 0.0003 0.0001 0.0007 0.0007 0.0001 0.0002 0.0001 0 0 0 0.0001
Peroxisomes 0.0323 0.0029 0.0106 0.0274 0.023 0.0139 0.0206 0.004 0.0111 0.0519 0.0037 0.0141 0.0088 0.0057 0.0007 0.0003 0.0015 0.0022
Punctate Nuclear 0.0064 0.0001 0.0007 0.015 0.0003 0.0006 0.0015 0.0001 0.0003 0.0015 0.0222 0.0001 0.0009 0.0002 0 0 0 0
Vacuole 0.0012 0.0001 0.0004 0.0011 0.0024 0.0004 0.0021 0.0138 0.0032 0.0014 0.0004 0.0002 0.0012 0.0001 0.0001 0 0 0.0013
Vacuole Periphery 0.0009 0 0.0001 0.0008 0.0017 0.0004 0.0009 0.0003 0.0006 0.001 0.0002 0.0002 0.0003 0 0 0 0 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 13.9231 10.372 15.7778 18.2679 15.7954 13.9154 20.8431 19.6417 19.0277 16.6858
Translational Efficiency 1.9913 2.5065 1.5799 1.2949 1.6442 2.401 1.4289 1.6195 1.8093 1.606

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1689 1566 2398 192 1754 2291 539 167 3443 3857 2937 359

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 780.60 897.61 1330.29 1196.44 926.35 969.58 1167.10 1048.43 854.85 940.36 1300.34 1127.59
Standard Deviation 124.70 171.27 202.45 149.25 122.28 153.32 180.31 142.27 143.37 164.69 208.37 163.64
Intensity Change Log 2 — 0.201505 0.769085 0.616093 — 0.065803 0.333299 0.178601 — 0.129448 0.549000 0.395211

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.792809* 0.876022* 0.902602* 0.745244* 0.808148* 0.848694* 0.602782* 0.745116* 0.800623* 0.859789* 0.847579* 0.745184*
Bud Neck 0.007549 0.002941 0.006182 0.011335 0.011362 0.005770 0.017983 0.018396 0.009491 0.004621 0.008348 0.014619
Bud Site 0.055062 0.021636 0.047045 0.130434* 0.058596 0.035751 0.112457* 0.103442* 0.056862 0.030020 0.059050 0.117878*
Cell Periphery 0.038059 0.008431 0.006953 0.034441 0.048959 0.032595 0.011890 0.011197 0.043612 0.022784 0.007859 0.023628
Cytoplasm 0.005989 0.003665 0.002224 0.002535 0.007190 0.005500 0.008021 0.002326 0.006601 0.004755 0.003287 0.002438
Cytoplasmic Foci 0.022916 0.007675 0.000692 0.005767 0.012212 0.006294 0.028792 0.012585 0.017463 0.006855 0.005849 0.008939
Eisosomes 0.020670 0.011722 0.027810 0.026154 0.035388 0.024186 0.009115 0.012096 0.028168 0.019126 0.024379 0.019614
Endoplasmic Reticulum 0.000549 0.000939 0.000712 0.004413 0.000280 0.000493 0.008199 0.002742 0.000412 0.000674 0.002086 0.003636
Endosome 0.002955 0.011514 0.000995 0.009216 0.000697 0.004996 0.032813 0.015646 0.001804 0.007642 0.006834 0.012207
Golgi 0.002696 0.011975 0.000262 0.009469 0.001211 0.005364 0.040248 0.004878 0.001939 0.008048 0.007600 0.007333
Lipid Particles 0.003388 0.007662 0.000888 0.006152 0.000409 0.002168 0.056292 0.004810 0.001871 0.004399 0.011056 0.005528
Mitochondria 0.005220 0.003392 0.000423 0.000334 0.002241 0.005171 0.001189 0.001854 0.003702 0.004448 0.000563 0.001041
Mitotic Spindle 0.000226 0.000217 0.000078 0.000204 0.000535 0.000343 0.000937 0.000444 0.000383 0.000292 0.000236 0.000316
None 0.000509 0.000139 0.000027 0.000019 0.000307 0.000101 0.000041 0.000015 0.000406 0.000116 0.000030 0.000017
Nuclear Periphery 0.000006 0.000003 0.000004 0.000006 0.000055 0.000008 0.000017 0.000007 0.000031 0.000006 0.000007 0.000006
Nuclear Periphery Foci 0.000047 0.000017 0.000009 0.000089 0.000069 0.000018 0.000358 0.000094 0.000058 0.000018 0.000073 0.000092
Nucleolus 0.000026 0.000002 0.000001 0.000001 0.000006 0.000034 0.000047 0.000001 0.000016 0.000021 0.000009 0.000001
Nucleus 0.000815 0.000037 0.000058 0.000047 0.000089 0.000375 0.002671 0.000040 0.000445 0.000238 0.000538 0.000044
Peroxisomes 0.040104 0.031788 0.002543 0.011024 0.011906 0.021590 0.056993 0.060796 0.025739 0.025731 0.012535 0.034177
Vacuole 0.000331 0.000150 0.000487 0.003098 0.000217 0.000310 0.009014 0.003493 0.000273 0.000245 0.002052 0.003282
Vacuole Periphery 0.000076 0.000071 0.000007 0.000017 0.000125 0.000239 0.000140 0.000021 0.000101 0.000171 0.000031 0.000019

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -9.15 -13.40 2.05 5.70 6.87 -4.91 12.10 2.59 4.22 -4.90 -9.67 -6.42 3.32 6.84 5.85
Bud Neck 4.69 1.26 -1.78 -4.21 -2.52 4.77 -2.03 -1.23 -2.25 -0.08 6.31 1.13 -1.77 -3.54 -2.16
Bud Site 7.40 1.46 -4.10 -5.99 -4.52 5.09 -5.06 -2.75 -4.21 0.44 8.40 -0.72 -4.91 -7.16 -4.64
Cell Periphery 9.96 10.91 0.50 -3.84 -4.09 4.58 10.10 9.54 6.41 0.18 8.66 16.72 4.63 -0.20 -4.06
Cytoplasm 1.99 4.06 2.28 0.69 -0.05 1.21 -0.69 3.74 3.18 3.75 2.02 3.64 4.06 2.40 1.34
Cytoplasmic Foci 6.88 11.01 5.17 0.69 -1.90 3.72 -4.68 -0.38 -1.64 2.88 7.84 7.68 2.73 -1.04 -1.19
Eisosomes 4.75 -3.00 -1.14 -3.06 0.32 4.11 9.41 5.96 3.40 -0.83 5.28 1.92 2.69 -0.15 1.44
Endoplasmic Reticulum -1.72 -1.16 -2.11 -1.89 -2.00 -1.60 -4.28 -1.61 -1.47 2.27 -2.11 -4.70 -2.66 -2.45 -1.21
Endosome -5.60 2.96 -1.96 0.67 -2.60 -5.46 -8.94 -2.41 -1.71 2.36 -7.35 -6.32 -3.10 -1.34 -1.58
Golgi -6.30 5.24 -1.84 0.64 -2.52 -4.99 -9.56 -2.81 0.34 8.29 -7.81 -6.65 -2.62 0.34 0.09
Lipid Particles -3.13 3.39 -1.04 0.54 -2.04 -4.14 -8.76 -2.62 -1.57 7.76 -3.96 -7.09 -2.30 -0.72 2.70
Mitochondria 1.92 6.73 6.81 4.73 0.75 -3.47 2.82 0.32 2.30 -0.54 -1.14 8.10 3.89 4.38 -0.82
Mitotic Spindle 0.07 3.37 0.25 0.11 -1.44 0.74 -1.18 0.34 -0.56 1.67 0.62 1.07 0.48 -0.21 -0.77
None 4.61 8.92 9.04 2.05 1.54 4.08 5.74 6.75 3.30 2.14 6.46 10.84 11.25 3.49 3.21
Nuclear Periphery 1.69 -0.36 -0.01 -1.97 0.37 0.94 0.74 0.95 0.23 2.95 0.96 0.87 0.95 -0.13 1.14
Nuclear Periphery Foci 1.38 1.33 -0.80 -1.51 -1.49 1.00 -3.47 -0.81 -1.32 1.57 1.45 -0.87 -1.05 -1.84 -0.68
Nucleolus 1.73 1.82 1.82 2.61 -0.10 -0.93 -1.83 1.32 1.06 1.98 -0.28 0.48 2.07 1.10 1.94
Nucleus 1.43 1.26 1.41 -0.86 1.67 -0.91 -1.78 2.20 1.05 1.81 0.62 -0.41 1.50 1.02 2.03
Peroxisomes 2.20 12.97 5.20 3.84 -1.75 -4.31 -7.43 -3.77 -3.01 -0.25 0.00 6.60 -1.24 -1.24 -3.19
Vacuole 1.10 -2.01 -1.13 -1.20 -0.63 -0.79 -4.00 -1.40 -1.37 1.78 0.29 -4.38 -1.79 -1.81 -0.15
Vacuole Periphery 0.16 1.71 1.46 3.80 -2.01 -0.85 -0.13 0.96 2.67 5.08 -0.91 1.19 1.40 3.11 2.10
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Actin assembly factor; C-terminal WCA domain activates Arp2/3 complex-mediated nucleation of branched actin filaments, polyproline domain nucleates actin filaments independent of Arp2/3; mutants are defective in endocytosis, bud site selection, cytokinesis; human homolog WAS (Wiskott-Aldrich Syndrome) implicated in severe immunodeficiency; human WAS complements yeast null mutant, but only in presence of WIPF1, which mediates localization of WAS to cortical patches
Localization
Cell Percentages actin (93%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Las17

Las17


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Las17-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available