Standard name
Human Ortholog
Description 3-phosphoserine aminotransferase; catalyzes the formation of phosphoserine from 3-phosphohydroxypyruvate, required for serine and glycine biosynthesis; regulated by the general control of amino acid biosynthesis mediated by Gcn4p; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0.05 0 0 0 0.05 0.08 0.06 0.1 0.09 0.11 0.07 0 0 0.05 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.99 0.98 0.98 0.93 0.94 0.84 0.83 0.81 0.81 0.82 0.78 0.98 0.99 1.0 1.0 1.0 0.99 0.98 0.98 0.95 0.95 0.96 0.95
Endoplasmic Reticulum 0 0 0 0 0 0 0.1 0.09 0.13 0.07 0.08 0.13 0 0 0 0 0.05 0.09 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 2 2 8 5 10 2 4 0 0 0 0 0 0 0 0 1 0 0 0
Bud 1 3 0 0 2 0 4 1 1 2 1 0 3 2 0 0 0 0 0 0 0 5 2 10
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 3 14 7 12 11 23 29 18 27 19 30 30 23 13 15 7 8 0 0 0 0 0 0
Cytoplasm 260 307 278 196 249 286 373 301 239 212 172 220 443 594 585 332 236 193 248 296 263 503 433 621
Endoplasmic Reticulum 0 1 3 1 7 8 43 33 38 19 17 38 2 1 2 13 12 18 0 0 4 1 2 6
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 2 3 0 1 0 0 0 1 0 0 0 0 0 0 0 0
Mitochondria 2 0 0 0 0 2 3 1 3 11 6 6 0 0 0 0 0 0 0 0 1 0 0 0
Nucleus 0 0 0 0 0 2 3 2 0 0 1 0 0 0 0 0 0 0 0 0 0 4 0 1
Nuclear Periphery 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 1 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Vac/Vac Membrane 0 1 0 0 4 4 8 15 2 4 3 6 2 0 0 0 0 0 0 0 1 5 5 5
Unique Cell Count 262 309 284 200 267 303 446 364 294 262 209 283 451 597 587 333 236 195 253 302 278 531 452 656
Labelled Cell Count 264 315 295 204 275 315 460 390 308 288 221 305 480 621 600 361 255 219 253 302 278 531 452 656


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 56.8 85.1 61.5 52.2 43.5 37.6 33.4 32.5 31.7 29.8 28.9 28.3 96.3 87.8 81.0 109.0 101.3 107.5 68.8 70.6 75.3
Std Deviation (1e-4) 12.4 17.7 12.2 11.7 9.9 8.5 7.7 8.2 7.9 7.9 7.6 8.0 33.8 21.9 19.7 30.2 27.3 34.6 17.5 17.8 21.3
Intensity Change (Log2) – – – -0.24 -0.5 -0.71 -0.88 -0.92 -0.96 -1.05 -1.09 -1.12 0.65 0.51 0.4 0.83 0.72 0.81 0.16 0.2 0.29


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -0.8 -0.2 -0.8 0.1 1.5 0.6 2.4 1.8 2.5 1.0 -0.7 -2.2 -0.2 -1.1 -0.4
Cytoplasm 0.1 -2.7 -2.2 -6.0 -6.2 -6.5 -6.5 -6.1 -7.3 0.3 2.2 2.6 2.1 2.2 0.9
Endoplasmic Reticulum 0 0 1.4 4.7 4.4 5.6 3.7 3.9 5.7 0 0 0 2.2 2.7 4.3
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 3.5 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 3.5 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 89.9289 108.2825 103.5878 100.748 92.7026 101.886 104.4257 133.3336 115.9162 105.0146 92.1307 120.3445 97.1214 126.0857 110.84 114.1106 111.9901 113.9193
Actin 0.0021 0.0002 0 0 0.0002 0.0002 0.0033 0.0001 0.0002 0.0001 0.0196 0.0001 0.0011 0.0001 0.0005 0 0 0.0002
Bud 0.0003 0.0001 0.0001 0 0.0002 0.0001 0.0006 0 0.0003 0.0003 0.0004 0 0.0008 0 0.0004 0 0 0.0001
Bud Neck 0.0004 0.0001 0.0003 0.0002 0.0004 0.0008 0.0003 0.0002 0.0003 0.0003 0.0008 0.0005 0.0003 0.0001 0.0002 0.0002 0.0002 0.0008
Bud Periphery 0.0002 0 0.0001 0 0.0002 0.0001 0.0009 0 0.0005 0.0002 0.0004 0 0.0006 0 0.0004 0 0 0
Bud Site 0.0002 0.0003 0 0 0.0002 0 0.0003 0 0.0001 0.0001 0.0005 0 0.0008 0 0.0004 0 0 0.0001
Cell Periphery 0.0015 0.0006 0.0003 0.0005 0.0017 0.0006 0.0025 0.0009 0.0025 0.0013 0.001 0.0002 0.0017 0.0001 0.0007 0.0005 0.0002 0.0003
Cytoplasm 0.9589 0.9946 0.9893 0.9927 0.9566 0.9821 0.9493 0.995 0.9683 0.9721 0.8782 0.9926 0.9278 0.9974 0.9631 0.9948 0.9887 0.9812
Cytoplasmic Foci 0.0012 0.0002 0.0001 0.0002 0.0047 0.0004 0.0055 0.0001 0.003 0.0008 0.0088 0.0002 0.0124 0.0001 0.0039 0.0001 0.0016 0.0024
Eisosomes 0.0001 0 0 0 0.0005 0 0.0001 0 0.0001 0 0.0005 0 0.0002 0 0.0003 0 0 0.0001
Endoplasmic Reticulum 0.0016 0.0006 0.0017 0.0005 0.0008 0.0009 0.0014 0.0008 0.0005 0.0011 0.0011 0.0006 0.0015 0.0001 0.0005 0.0005 0.0002 0.0003
Endosome 0.0007 0 0.0001 0.0001 0.0004 0.0004 0.0012 0 0.0002 0.0003 0.0029 0 0.0031 0 0.0003 0 0.0001 0.0003
Golgi 0.0002 0 0 0 0.0002 0 0.0002 0 0 0 0.0034 0 0.0004 0 0.0001 0 0 0.0001
Lipid Particles 0.0007 0.0001 0 0.0002 0.0033 0.0002 0.0018 0.0001 0.0009 0.0005 0.0046 0.0001 0.0024 0 0.0014 0 0.0004 0.0012
Mitochondria 0.0008 0.0001 0.0001 0 0.0018 0.0006 0.0019 0 0.0012 0.0001 0.0044 0 0.0029 0 0.0072 0 0.0001 0.0004
None 0.0016 0.0004 0.0002 0.0001 0.004 0.0004 0.004 0.0001 0.0047 0.0025 0.0185 0.0003 0.0108 0.0001 0.0062 0 0.0015 0.0015
Nuclear Periphery 0.0025 0.0005 0.001 0.002 0.0015 0.0022 0.0041 0.0004 0.001 0.0028 0.0032 0.0013 0.0036 0.0003 0.0016 0.0006 0.0012 0.001
Nucleolus 0.0069 0.0001 0.0002 0 0.008 0.0011 0.006 0 0.0049 0.0006 0.0096 0.0001 0.0038 0 0.0042 0 0.0006 0.0036
Nucleus 0.0084 0.001 0.0027 0.002 0.0042 0.0042 0.0093 0.0011 0.0032 0.0047 0.0117 0.0029 0.0093 0.0013 0.0035 0.0027 0.0031 0.0037
Peroxisomes 0.0001 0 0 0 0.0002 0 0.0003 0 0.0001 0 0.0009 0 0.0004 0 0.0003 0 0 0.0001
Punctate Nuclear 0.0004 0 0 0 0.0008 0.0001 0.0016 0 0.0004 0.0002 0.0023 0.0001 0.0061 0 0.001 0 0.0003 0.0008
Vacuole 0.0093 0.0008 0.0029 0.0013 0.0088 0.0043 0.0045 0.0011 0.0063 0.011 0.0219 0.0008 0.0081 0.0002 0.0025 0.0004 0.0013 0.0011
Vacuole Periphery 0.002 0.0001 0.0009 0.0002 0.0013 0.0014 0.0011 0 0.0012 0.0011 0.0053 0.0001 0.0019 0 0.0014 0 0.0003 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 344.5817 187.644 156.4909 207.6497 222.7805 313.7578 207.9857 205.9608 205.2596 233.1686
Translational Efficiency 1.8083 2.1129 1.9324 1.5835 1.6919 2.1993 1.7795 1.8168 1.8385 1.7296

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1767 203 1957 993 1456 1692 292 577 3223 1895 2249 1570

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 4548.29 6766.53 2985.47 3169.10 4880.04 5181.19 3463.39 3699.05 4698.16 5351.02 3047.52 3363.87
Standard Deviation 1139.78 1254.23 584.39 802.02 1156.94 1187.73 550.23 849.23 1159.38 1291.70 601.90 858.59
Intensity Change Log 2 — 0.573092 -0.607366 -0.521251 — 0.086391 -0.494708 -0.399738 — 0.341661 -0.547958 -0.457081

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000031 0.000118 0.000091 0.000304 0.000072 0.000094 0.000301 0.000337 0.000049 0.000096 0.000118 0.000316
Bud Neck 0.000151 0.001397 0.001718 0.001468 0.000565 0.000591 0.005593 0.002656 0.000338 0.000678 0.002221 0.001904
Bud Site 0.000582 0.002992 0.003787 0.009575 0.001409 0.002205 0.006197 0.014326 0.000956 0.002289 0.004100 0.011321
Cell Periphery 0.001362 0.015996 0.000720 0.003567 0.002005 0.001630 0.004057 0.001921 0.001652 0.003169 0.001153 0.002962
Cytoplasm 0.946235* 0.801578* 0.843569* 0.876905* 0.877749* 0.896759* 0.764857* 0.829631* 0.915296* 0.886563* 0.833350* 0.859531*
Cytoplasmic Foci 0.001972 0.021836 0.004329 0.005530 0.008240 0.007088 0.019692 0.009105 0.004804 0.008668 0.006324 0.006844
Eisosomes 0.000000 0.000003 0.000001 0.000003 0.000001 0.000001 0.000005 0.000005 0.000001 0.000001 0.000002 0.000004
Endoplasmic Reticulum 0.000060 0.000055 0.000298 0.000030 0.000068 0.000130 0.000058 0.000098 0.000064 0.000122 0.000267 0.000055
Endosome 0.000056 0.000310 0.000934 0.000321 0.000168 0.000299 0.000617 0.002632 0.000107 0.000301 0.000893 0.001170
Golgi 0.003401 0.011912 0.001685 0.000973 0.008858 0.003873 0.003827 0.003058 0.005866 0.004734 0.001963 0.001739
Lipid Particles 0.001716 0.017346 0.003732 0.006425 0.001613 0.002753 0.013611 0.005332 0.001669 0.004316 0.005015 0.006023
Mitochondria 0.000069 0.000176 0.000109 0.000058 0.000225 0.000175 0.000052 0.000650 0.000139 0.000175 0.000102 0.000275
Mitotic Spindle 0.000115 0.001495 0.000534 0.000251 0.001543 0.001638 0.001291 0.006424 0.000760 0.001623 0.000632 0.002520
None 0.006858 0.007670 0.001421 0.004087 0.006891 0.007877 0.004210 0.003404 0.006873 0.007855 0.001783 0.003836
Nuclear Periphery 0.000093 0.000655 0.000173 0.000078 0.000271 0.000148 0.000360 0.000170 0.000173 0.000202 0.000197 0.000112
Nuclear Periphery Foci 0.002191 0.008640 0.002407 0.002348 0.002874 0.002558 0.008466 0.003046 0.002500 0.003210 0.003193 0.002605
Nucleolus 0.000045 0.000089 0.000219 0.000096 0.000131 0.000161 0.000129 0.000180 0.000084 0.000153 0.000208 0.000127
Nucleus 0.024116 0.050549 0.031816 0.042940 0.066509 0.052832 0.075592 0.053159 0.043267 0.052587 0.037500 0.046695
Peroxisomes 0.000007 0.000021 0.000076 0.000065 0.000006 0.000021 0.000040 0.000106 0.000006 0.000021 0.000072 0.000080
Vacuole 0.008692 0.049093 0.100180 0.044528 0.013162 0.017140 0.090244 0.060617 0.010711 0.020563 0.098890 0.050441
Vacuole Periphery 0.002250 0.008068 0.002199 0.000449 0.007640 0.002025 0.000801 0.003143 0.004685 0.002672 0.002018 0.001439

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.11 -7.80 -3.48 -2.29 -2.48 -1.52 -7.54 -5.65 -4.67 2.21 -3.30 -9.28 -4.69 -3.73 -2.78
Bud Neck -3.36 -10.36 -8.79 -0.41 3.30 -1.31 -9.19 -8.34 -7.88 5.65 -4.89 -12.65 -11.58 -8.01 3.70
Bud Site -5.25 -13.76 -8.01 -5.33 -4.22 -2.60 -9.16 -4.85 -4.20 -0.69 -4.71 -14.49 -9.03 -7.00 -4.56
Cell Periphery -7.32 3.97 -5.07 6.36 -7.40 1.37 -4.19 1.13 -0.23 5.08 -5.38 3.92 -3.98 1.78 -6.77
Cytoplasm 9.30 25.33 18.15 -3.05 -5.62 -1.64 14.58 11.59 12.93 -6.57 8.69 23.47 19.02 9.93 -4.49
Cytoplasmic Foci -5.54 -10.61 -6.74 4.06 -1.82 0.49 -8.64 -3.71 -4.27 6.60 -5.85 -6.66 -6.28 0.42 -0.81
Eisosomes -3.38 -9.31 -10.38 -0.30 -4.59 -0.98 -9.30 -5.94 -5.67 2.88 -4.10 -11.63 -10.60 -7.90 -3.61
Endoplasmic Reticulum 0.10 -2.17 1.39 2.84 2.50 -0.95 -0.91 0.36 1.07 1.08 -1.03 -2.17 1.51 1.48 2.45
Endosome -4.11 -7.04 -1.83 0.43 4.20 -1.79 -6.91 -2.01 -1.71 -0.61 -2.95 -7.46 -2.47 -1.37 1.54
Golgi -4.35 5.43 8.25 5.72 4.83 5.77 5.97 7.65 3.73 2.75 2.08 9.53 10.66 8.65 3.34
Lipid Particles -5.42 -6.54 -7.25 3.16 -3.56 -3.02 -7.90 -6.45 -4.78 4.43 -5.77 -10.12 -9.61 -4.22 -2.00
Mitochondria -1.07 -2.71 0.92 1.18 3.64 1.02 4.60 -0.48 -0.88 -1.70 -0.70 1.94 -0.04 0.46 -0.79
Mitotic Spindle -2.00 -4.48 -1.67 1.60 1.74 -0.52 -1.02 -1.66 -1.44 -0.88 -1.97 -1.01 -1.69 -0.13 -1.30
None -0.34 9.54 4.54 2.93 -3.77 -0.88 5.84 7.89 6.28 0.44 -0.94 13.54 7.39 6.11 -4.17
Nuclear Periphery -4.83 -3.17 -1.06 4.71 2.90 3.30 -4.98 0.89 -2.29 5.61 -2.10 -2.43 0.92 3.09 3.00
Nuclear Periphery Foci -5.01 -1.92 -2.28 4.37 0.14 0.31 -6.94 -2.61 -3.68 5.74 -2.76 -3.18 -3.09 0.03 1.01
Nucleolus -2.09 -9.82 -4.43 0.15 8.30 -0.38 -0.58 -0.61 -0.04 -0.17 -1.26 -5.90 -1.36 0.75 6.23
Nucleus -5.33 -8.44 -11.04 -0.14 -3.30 3.06 -4.29 -1.87 -4.29 2.74 -5.53 -2.94 -7.34 -1.89 -4.05
Peroxisomes -2.15 -8.90 -6.86 -3.87 1.29 -2.79 -9.51 -5.88 -4.07 -0.82 -3.02 -10.03 -9.05 -5.30 0.80
Vacuole -5.00 -25.62 -15.18 -0.69 10.84 -3.22 -12.38 -14.27 -12.87 4.14 -6.84 -27.76 -20.28 -13.93 9.43
Vacuole Periphery -1.42 0.33 2.89 1.92 11.18 5.14 6.64 5.10 0.72 -1.12 2.55 4.73 6.18 3.18 4.08
Standard name
Human Ortholog
Description 3-phosphoserine aminotransferase; catalyzes the formation of phosphoserine from 3-phosphohydroxypyruvate, required for serine and glycine biosynthesis; regulated by the general control of amino acid biosynthesis mediated by Gcn4p; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-2

Ser1

Ser1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ser1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available