Standard name | |
---|---|
Human Ortholog | |
Description | GTP binding protein (mammalian Ranp homolog); involved in the maintenance of nuclear organization, RNA processing and transport; interacts with Kap121p, Kap123p and Pdr6p (karyophilin betas); not required for viability; protein abundance increases in response to DNA replication stress; GSP2 has a paralog, GSP1, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 1 |
Bud | 0 | 1 | 0 | 0 | 0 | 0 | 3 | 4 | 5 | 4 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 5 | 3 | 3 | 11 | 23 | 34 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 1 | 4 | 4 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 2 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 209 | 128 | 155 | 74 | 90 | 102 | 78 | 113 | 73 | 84 | 67 | 68 | 104 | 117 | 109 | 126 | 81 | 105 | 218 | 115 | 154 | 215 | 182 | 184 |
Endoplasmic Reticulum | 2 | 2 | 0 | 1 | 2 | 1 | 0 | 4 | 1 | 2 | 4 | 3 | 2 | 3 | 3 | 7 | 15 | 7 | 1 | 2 | 7 | 5 | 4 | 6 |
Endosome | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 6 | 0 | 2 | 2 | 16 | 12 | 1 | 0 | 0 | 5 | 1 | 7 | 6 | 6 | 7 |
Golgi | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 3 | 5 | 3 | 11 | 4 | 1 | 1 | 0 | 3 | 1 | 1 |
Mitochondria | 4 | 1 | 8 | 10 | 11 | 25 | 32 | 49 | 43 | 51 | 60 | 47 | 1 | 0 | 0 | 4 | 1 | 0 | 3 | 0 | 6 | 11 | 11 | 8 |
Nucleus | 14 | 5 | 9 | 51 | 38 | 56 | 71 | 117 | 98 | 109 | 76 | 79 | 51 | 42 | 47 | 17 | 6 | 5 | 33 | 47 | 38 | 50 | 69 | 80 |
Nuclear Periphery | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 6 | 2 | 3 | 2 | 5 | 0 | 2 | 0 | 0 | 0 | 0 | 6 | 1 | 12 | 1 | 2 | 3 |
Nucleolus | 1 | 0 | 0 | 1 | 0 | 2 | 7 | 15 | 5 | 10 | 6 | 3 | 0 | 5 | 8 | 1 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 8 | 4 | 9 | 9 | 15 | 9 |
Vac/Vac Membrane | 103 | 71 | 128 | 134 | 199 | 148 | 232 | 262 | 162 | 166 | 90 | 95 | 281 | 450 | 442 | 276 | 251 | 233 | 32 | 23 | 41 | 75 | 123 | 138 |
Unique Cell Count | 280 | 182 | 250 | 241 | 312 | 301 | 378 | 512 | 341 | 383 | 259 | 275 | 376 | 582 | 585 | 367 | 309 | 307 | 325 | 208 | 288 | 404 | 459 | 489 |
Labelled Cell Count | 334 | 208 | 301 | 273 | 342 | 335 | 423 | 571 | 391 | 438 | 309 | 307 | 441 | 640 | 629 | 438 | 365 | 354 | 325 | 208 | 288 | 404 | 459 | 489 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.1 | 5.9 | 5.1 | 6.1 | 8.4 | 10.1 | 9.9 | 10.8 | 10.5 | 10.7 | 11.2 | 12.3 | 8.9 | 11.8 | 13.4 | 23.0 | 23.5 | 23.7 | 7.7 | 8.2 | 8.8 |
Std Deviation (1e-4) | 0.8 | 0.9 | 1.2 | 1.8 | 2.1 | 2.8 | 3.3 | 3.7 | 3.9 | 4.3 | 4.6 | 5.2 | 2.2 | 4.0 | 4.3 | 8.0 | 7.0 | 6.9 | 3.7 | 3.4 | 2.1 |
Intensity Change (Log2) | – | – | – | 0.27 | 0.74 | 1.0 | 0.97 | 1.09 | 1.06 | 1.08 | 1.15 | 1.29 | 0.82 | 1.23 | 1.41 | 2.18 | 2.22 | 2.23 | 0.6 | 0.7 | 0.8 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -6.9 | -7.9 | -6.6 | -10.5 | -10.8 | -10.0 | -10.2 | -8.2 | -8.6 | -8.5 | -11.8 | -12.3 | -6.8 | -8.5 | -6.5 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.5 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.2 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0.6 | 0.2 | 2.5 | 2.6 | 3.1 | 4.0 | 4.3 | 6.6 | 5.2 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 5.9 | 3.7 | 5.4 | 5.6 | 6.7 | 7.8 | 7.9 | 7.8 | 7.7 | 4.1 | 2.0 | 2.3 | 0.6 | -1.2 | -1.5 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 1.0 | 3.0 | -0.5 | 2.5 | 0 | -0.9 | -1.9 | -3.7 | -3.9 | 6.1 | 7.5 | 6.9 | 6.2 | 7.6 | 6.1 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Actin | 0.0083 | 0.0002 | 0.0007 | 0.0178 | 0.0064 | 0.0003 | 0.0084 | 0.0016 | 0.0042 | 0.0016 | 0.0209 | 0.0007 | 0.2742 | 0.0006 | 0.0151 | 0.0006 | 0.0002 | 0.0008 |
Bud | 0.0001 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0006 | 0.0008 | 0.0007 | 0.0021 | 0.0005 | 0.0001 | 0.0044 | 0.0002 | 0.0004 | 0 | 0.0001 | 0.0002 |
Bud Neck | 0.0016 | 0.0004 | 0.0006 | 0.0022 | 0.0013 | 0.0026 | 0.0054 | 0.0005 | 0.0005 | 0.0032 | 0.0009 | 0.0011 | 0.0019 | 0.0003 | 0.0005 | 0.0003 | 0.0005 | 0.002 |
Bud Periphery | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0014 | 0.0004 | 0.0006 | 0.0011 | 0.0006 | 0.0001 | 0.01 | 0.0001 | 0.0002 | 0 | 0 | 0.0002 |
Bud Site | 0.0007 | 0.0005 | 0.0002 | 0.0002 | 0.0002 | 0.0009 | 0.004 | 0.0021 | 0.0045 | 0.0134 | 0.0009 | 0.0001 | 0.0074 | 0.0006 | 0.0032 | 0 | 0.0001 | 0.0001 |
Cell Periphery | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0009 | 0.0001 | 0.0001 | 0 | 0 | 0 |
Cytoplasm | 0.0544 | 0.1541 | 0.1016 | 0.0676 | 0.0646 | 0.0764 | 0.1097 | 0.3347 | 0.1467 | 0.1091 | 0.1848 | 0.126 | 0.1506 | 0.4584 | 0.31 | 0.2063 | 0.1512 | 0.1237 |
Cytoplasmic Foci | 0.0059 | 0.0006 | 0.0005 | 0.0049 | 0.0117 | 0.001 | 0.0069 | 0.0038 | 0.0044 | 0.0193 | 0.0091 | 0.0032 | 0.0398 | 0.0015 | 0.0336 | 0.0002 | 0.0002 | 0.0041 |
Eisosomes | 0.0003 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0.0024 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0069 | 0.0045 | 0.0059 | 0.0221 | 0.0027 | 0.0045 | 0.0124 | 0.0126 | 0.0111 | 0.0081 | 0.007 | 0.0079 | 0.013 | 0.0238 | 0.01 | 0.0212 | 0.0022 | 0.0046 |
Endosome | 0.0128 | 0.0022 | 0.0034 | 0.0091 | 0.0115 | 0.0045 | 0.0231 | 0.0058 | 0.0103 | 0.0398 | 0.0197 | 0.0048 | 0.0192 | 0.0049 | 0.0548 | 0.0022 | 0.0008 | 0.0148 |
Golgi | 0.002 | 0 | 0 | 0.0011 | 0.0168 | 0.0001 | 0.002 | 0.0002 | 0.0024 | 0.0124 | 0.0135 | 0.0006 | 0.0143 | 0.0001 | 0.0041 | 0 | 0 | 0.0073 |
Lipid Particles | 0.0051 | 0 | 0 | 0.0008 | 0.0262 | 0.0001 | 0.0021 | 0.0001 | 0.0005 | 0.017 | 0.0053 | 0.0009 | 0.007 | 0.0003 | 0.0077 | 0 | 0 | 0.0345 |
Mitochondria | 0.0014 | 0.0005 | 0.0004 | 0.0007 | 0.0029 | 0.0003 | 0.0045 | 0.0007 | 0.0016 | 0.0064 | 0.0045 | 0.0007 | 0.0312 | 0.0007 | 0.0015 | 0.0005 | 0.0007 | 0.0091 |
None | 0.004 | 0.0039 | 0.0009 | 0.002 | 0.0005 | 0.002 | 0.0177 | 0.049 | 0.0206 | 0.0026 | 0.0418 | 0.0213 | 0.0063 | 0.0044 | 0.0047 | 0.0007 | 0.0012 | 0.0022 |
Nuclear Periphery | 0.1504 | 0.1 | 0.1773 | 0.1258 | 0.0591 | 0.0913 | 0.1809 | 0.1092 | 0.1766 | 0.1467 | 0.0724 | 0.106 | 0.0332 | 0.0936 | 0.1577 | 0.2496 | 0.0365 | 0.0585 |
Nucleolus | 0.0015 | 0.0006 | 0.0006 | 0.0005 | 0.0009 | 0.0021 | 0.0049 | 0.0009 | 0.0008 | 0.0033 | 0.0006 | 0.0006 | 0.0006 | 0.0004 | 0.0009 | 0.0001 | 0.0005 | 0.001 |
Nucleus | 0.7258 | 0.7248 | 0.6958 | 0.7289 | 0.778 | 0.7951 | 0.5906 | 0.4611 | 0.5982 | 0.5646 | 0.5848 | 0.7058 | 0.3392 | 0.3972 | 0.3573 | 0.5123 | 0.8018 | 0.7238 |
Peroxisomes | 0.0012 | 0 | 0 | 0.0008 | 0.0078 | 0 | 0.0005 | 0.0002 | 0.0021 | 0.0049 | 0.0034 | 0.0003 | 0.0296 | 0 | 0.0118 | 0 | 0 | 0.0028 |
Punctate Nuclear | 0.0145 | 0.0029 | 0.0083 | 0.0126 | 0.0068 | 0.0158 | 0.0118 | 0.0101 | 0.0089 | 0.032 | 0.0262 | 0.0171 | 0.004 | 0.0075 | 0.0151 | 0.0019 | 0.0025 | 0.0061 |
Vacuole | 0.0021 | 0.0034 | 0.0021 | 0.0017 | 0.0019 | 0.0018 | 0.0089 | 0.0047 | 0.0037 | 0.0091 | 0.0019 | 0.0017 | 0.004 | 0.004 | 0.0084 | 0.0019 | 0.0011 | 0.0024 |
Vacuole Periphery | 0.0012 | 0.0009 | 0.0011 | 0.001 | 0.0006 | 0.0007 | 0.0039 | 0.0013 | 0.0016 | 0.0028 | 0.0009 | 0.001 | 0.0067 | 0.0012 | 0.003 | 0.002 | 0.0005 | 0.0018 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 29.1246 | 32.7304 | 30.9112 | 47.6966 | 44.2346 | 48.2368 | 42.1193 | 53.3019 | 69.1604 | 57.4238 |
Translational Efficiency | 2.0536 | 1.5258 | 1.6945 | 1.5356 | 1.393 | 1.6018 | 1.5162 | 1.3867 | 1.412 | 1.4719 |
Dataset | Hit |
---|---|
Protein Concentration | – |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
241 | 1603 | 980 | 1331 | 1392 | 2472 | 2863 | 1347 | 1633 | 4075 | 3843 | 2678 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 775.89 | 818.02 | 1253.79 | 1265.75 | 715.35 | 755.53 | 950.87 | 1427.37 | 724.28 | 780.11 | 1028.12 | 1347.04 |
Standard Deviation | 156.29 | 122.21 | 229.33 | 345.41 | 130.12 | 114.92 | 160.41 | 275.79 | 136.01 | 121.73 | 223.64 | 322.62 |
Intensity Change Log 2 | — | 0.076284 | 0.692372 | 0.706068 | — | 0.078840 | 0.410599 | 0.996638 | — | 0.077511 | 0.564044 | 0.852766 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.003003 | 0.000188 | 0.000607 | 0.001245 | 0.000395 | 0.000162 | 0.000146 | 0.000631 | 0.000780 | 0.000172 | 0.000264 | 0.000936 |
Bud Neck | 0.003198 | 0.006966 | 0.008980 | 0.017549 | 0.003526 | 0.016683 | 0.011417 | 0.013171 | 0.003478 | 0.012861 | 0.010795 | 0.015347 |
Bud Site | 0.006989 | 0.002650 | 0.001127 | 0.011910 | 0.001041 | 0.006293 | 0.003127 | 0.002136 | 0.001919 | 0.004860 | 0.002617 | 0.006994 |
Cell Periphery | 0.000700 | 0.000099 | 0.000110 | 0.000243 | 0.000228 | 0.000133 | 0.000083 | 0.000115 | 0.000298 | 0.000120 | 0.000090 | 0.000178 |
Cytoplasm | 0.329946* | 0.439220* | 0.059408 | 0.143153* | 0.440119* | 0.458300* | 0.362950* | 0.069875 | 0.423860* | 0.450794* | 0.285544* | 0.106295* |
Cytoplasmic Foci | 0.050368 | 0.061864 | 0.000306 | 0.006363 | 0.087745 | 0.108377 | 0.001701 | 0.003107 | 0.082229 | 0.090080 | 0.001346 | 0.004725 |
Eisosomes | 0.000122 | 0.000041 | 0.000008 | 0.000021 | 0.000225 | 0.000054 | 0.000011 | 0.000010 | 0.000210 | 0.000048 | 0.000010 | 0.000015 |
Endoplasmic Reticulum | 0.034862 | 0.007003 | 0.006725 | 0.006744 | 0.002161 | 0.004073 | 0.003165 | 0.009168 | 0.006987 | 0.005225 | 0.004073 | 0.007964 |
Endosome | 0.010346 | 0.005440 | 0.000793 | 0.011943 | 0.006221 | 0.008298 | 0.000959 | 0.004749 | 0.006830 | 0.007174 | 0.000917 | 0.008324 |
Golgi | 0.001111 | 0.000629 | 0.000104 | 0.010542 | 0.002522 | 0.001752 | 0.000104 | 0.001359 | 0.002314 | 0.001310 | 0.000104 | 0.005923 |
Lipid Particles | 0.016823 | 0.006692 | 0.000312 | 0.003205 | 0.009914 | 0.009493 | 0.000434 | 0.000516 | 0.010934 | 0.008391 | 0.000403 | 0.001853 |
Mitochondria | 0.003611 | 0.003128 | 0.002149 | 0.017433 | 0.002977 | 0.009344 | 0.002093 | 0.003864 | 0.003071 | 0.006899 | 0.002108 | 0.010608 |
Mitotic Spindle | 0.002992 | 0.004685 | 0.003572 | 0.154091* | 0.004409 | 0.008782 | 0.017420 | 0.037387 | 0.004200 | 0.007170 | 0.013889 | 0.095390 |
None | 0.023947 | 0.007132 | 0.005462 | 0.023784 | 0.026158 | 0.006658 | 0.011077 | 0.006924 | 0.025832 | 0.006844 | 0.009645 | 0.015304 |
Nuclear Periphery | 0.027927 | 0.008616 | 0.014581 | 0.019670 | 0.004517 | 0.004713 | 0.009673 | 0.042699 | 0.007972 | 0.006249 | 0.010925 | 0.031253 |
Nuclear Periphery Foci | 0.061793 | 0.010079 | 0.007177 | 0.014337 | 0.001812 | 0.002900 | 0.002458 | 0.016783 | 0.010665 | 0.005724 | 0.003661 | 0.015567 |
Nucleolus | 0.009792 | 0.003633 | 0.004145 | 0.007459 | 0.002364 | 0.003491 | 0.001795 | 0.007617 | 0.003460 | 0.003547 | 0.002394 | 0.007539 |
Nucleus | 0.385614* | 0.397365* | 0.874636* | 0.492749* | 0.379791* | 0.305688* | 0.555183* | 0.766097* | 0.380650* | 0.341751* | 0.636647* | 0.630239* |
Peroxisomes | 0.002826 | 0.002295 | 0.000107 | 0.000695 | 0.002498 | 0.005038 | 0.000156 | 0.000158 | 0.002547 | 0.003959 | 0.000143 | 0.000425 |
Vacuole | 0.022520 | 0.031271 | 0.009405 | 0.037948 | 0.021031 | 0.038126 | 0.015576 | 0.012954 | 0.021251 | 0.035429 | 0.014002 | 0.025377 |
Vacuole Periphery | 0.001512 | 0.001004 | 0.000287 | 0.018917 | 0.000343 | 0.001643 | 0.000472 | 0.000679 | 0.000516 | 0.001392 | 0.000425 | 0.009744 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 1.47 | 1.12 | 1.10 | -6.62 | -0.31 | 0.74 | 0.46 | -1.83 | -17.04 | -15.41 | 1.56 | 0.97 | -0.39 | -12.95 | -8.78 |
Bud Neck | -3.82 | -9.77 | -14.79 | -11.80 | -6.17 | -9.70 | -9.95 | -15.64 | -7.95 | -9.76 | -9.51 | -12.38 | -20.42 | -12.79 | -11.76 |
Bud Site | 2.21 | 2.42 | -0.35 | -5.97 | -6.54 | -7.85 | -4.79 | -7.66 | 1.84 | -3.45 | -5.25 | -1.81 | -7.55 | -2.58 | -6.86 |
Cell Periphery | 4.03 | 3.78 | 3.25 | -5.09 | -3.21 | 2.67 | 4.90 | 2.27 | -1.17 | -9.59 | 4.89 | 5.95 | 3.15 | -4.01 | -7.89 |
Cytoplasm | -5.66 | 15.42 | 12.34 | 42.18 | -11.47 | -1.80 | 18.67 | 51.45 | 67.06* | 46.74* | -3.04 | 25.95 | 48.67 | 75.74* | 33.60 |
Cytoplasmic Foci | -2.11 | 8.64 | 8.29 | 20.37 | -4.41 | -3.98 | 23.53 | 23.32 | 32.86 | -0.22 | -1.87 | 24.88 | 24.59 | 38.26 | -1.80 |
Eisosomes | 2.08 | 2.84 | 2.47 | 6.26 | -8.80 | 3.47 | 4.22 | 4.19 | 15.96 | -1.46 | 3.79 | 4.57 | 4.41 | 15.98 | -7.45 |
Endoplasmic Reticulum | 6.20 | 6.08 | 6.38 | 0.87 | 1.83 | -5.17 | -10.13 | -15.28 | -9.56 | -8.84 | 2.02 | 1.57 | -1.43 | -6.23 | -6.25 |
Endosome | 1.79 | 3.51 | 2.43 | 2.50 | -4.89 | -2.24 | 6.29 | 3.15 | 6.90 | -3.93 | -0.52 | 7.22 | 3.80 | 7.08 | -6.21 |
Golgi | 1.56 | 3.11 | -1.11 | -1.81 | -2.38 | 0.90 | 3.07 | 2.21 | 2.86 | -2.59 | 1.44 | 3.27 | 1.10 | -0.25 | -3.03 |
Lipid Particles | 4.29 | 7.14 | 6.56 | 8.37 | -4.49 | 0.48 | 11.03 | 10.69 | 14.51 | -2.73 | 2.87 | 12.99 | 11.93 | 16.25 | -4.97 |
Mitochondria | 0.65 | 1.44 | -2.53 | -3.02 | -3.88 | -5.31 | 0.76 | -1.82 | 5.37 | -8.88 | -4.18 | 0.87 | -2.83 | 1.87 | -6.16 |
Mitotic Spindle | -0.90 | -2.65 | -16.66 | -16.76 | -15.56 | -2.33 | -7.27 | -10.07 | -9.12 | -6.26 | -2.05 | -7.48 | -19.06 | -18.58 | -15.37 |
None | 4.42 | 4.75 | 1.84 | -7.49 | -8.36 | 6.03 | 4.90 | 5.85 | -1.05 | 5.66 | 6.79 | 5.94 | 4.87 | -7.02 | -3.99 |
Nuclear Periphery | 3.83 | 1.80 | 0.84 | -6.70 | -1.92 | -0.36 | -10.56 | -13.31 | -13.39 | -9.36 | 1.48 | -6.87 | -12.93 | -15.04 | -9.11 |
Nuclear Periphery Foci | 5.60 | 5.62 | 5.61 | -0.03 | -0.25 | -2.74 | -3.63 | -8.68 | -7.94 | -7.41 | 2.88 | 3.29 | -2.41 | -6.71 | -7.22 |
Nucleolus | 3.45 | 0.82 | 0.40 | -5.06 | -0.61 | -2.73 | -2.27 | -10.07 | -9.13 | -9.53 | -0.70 | -2.19 | -10.64 | -10.44 | -9.57 |
Nucleus | -0.42 | -23.44 | -9.27 | -16.33 | 24.82 | 8.30 | -25.61 | -31.70 | -44.27 | -12.85 | 4.92 | -35.43 | -29.58 | -42.10 | 2.00 |
Peroxisomes | 0.45 | 2.80 | 2.67 | 4.65 | -2.20 | -4.36 | 5.42 | 5.20 | 12.00 | -2.05 | -2.94 | 6.06 | 5.76 | 12.41 | -2.75 |
Vacuole | -2.77 | 1.43 | -8.55 | -8.24 | -12.86 | -7.82 | -5.74 | -2.11 | 4.98 | 3.05 | -8.38 | -4.17 | -10.30 | -4.15 | -7.30 |
Vacuole Periphery | 1.67 | 6.10 | -2.23 | -2.74 | -3.49 | -4.37 | 0.51 | -1.86 | 3.73 | -1.89 | -4.05 | 3.09 | -3.28 | -1.38 | -3.69 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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