Standard name | |
---|---|
Human Ortholog | |
Description | Protein of unknown function; may be involved in positive regulation of 1,3-beta-glucan synthesis and the Pkc1p-MAPK pathway; multicopy suppressor of temperature-sensitive mutations in CDC24 and CDC42, and of mutations in BEM4; potential Cdc28p substrate; relocalizes from bud neck to cytoplasm upon DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 2 | 22 | 21 | 24 | 9 | 19 | 1 | 4 | – | 3 | 5 | 11 | 39 | 30 | 43 | 61 | 73 | 66 | 11 | 11 | 24 | 14 | 50 | 64 |
Bud | 0 | 10 | 18 | 41 | 75 | 61 | 83 | 74 | – | 60 | 56 | 72 | 20 | 14 | 22 | 27 | 45 | 43 | 9 | 18 | 12 | 17 | 68 | 77 |
Bud Neck | 0 | 0 | 2 | 2 | 3 | 8 | 12 | 3 | – | 10 | 4 | 4 | 6 | 2 | 8 | 1 | 15 | 4 | 10 | 21 | 15 | 24 | 66 | 51 |
Bud Site | 2 | 2 | 4 | 34 | 42 | 53 | 32 | 18 | – | 28 | 35 | 45 | 4 | 3 | 3 | 18 | 20 | 17 | – | – | – | – | – | – |
Cell Periphery | 1 | 2 | 4 | 19 | 5 | 8 | 9 | 2 | – | 11 | 10 | 17 | 12 | 24 | 34 | 18 | 25 | 30 | 1 | 0 | 2 | 2 | 5 | 8 |
Cytoplasm | 32 | 35 | 83 | 193 | 203 | 254 | 324 | 372 | – | 304 | 156 | 232 | 238 | 353 | 443 | 146 | 138 | 150 | 43 | 36 | 60 | 54 | 95 | 151 |
Endoplasmic Reticulum | 1 | 4 | 3 | 4 | 5 | 12 | 4 | 2 | – | 1 | 2 | 10 | 1 | 1 | 7 | 9 | 7 | 13 | 1 | 0 | 3 | 2 | 10 | 9 |
Endosome | 3 | 2 | 1 | 14 | 47 | 5 | 8 | 15 | – | 4 | 1 | 6 | 9 | 8 | 10 | 31 | 54 | 44 | 3 | 2 | 2 | 4 | 5 | 7 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 7 | 5 | 14 | 0 | 0 | 0 | 0 | 0 | 1 |
Mitochondria | 5 | 0 | 15 | 29 | 79 | 151 | 260 | 347 | – | 274 | 309 | 394 | 0 | 0 | 0 | 25 | 16 | 15 | 2 | 3 | 7 | 9 | 14 | 10 |
Nucleus | 0 | 0 | 1 | 1 | 6 | 4 | 4 | 8 | – | 4 | 5 | 6 | 1 | 0 | 2 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 2 | – | 3 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 1 | 6 | 7 | 9 | 17 | – | 8 | 5 | 6 | 0 | 0 | 0 | 3 | 6 | 6 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 2 | 2 | 42 | 53 | 30 | 24 | 26 | – | 21 | 17 | 51 | 11 | 15 | 19 | 58 | 78 | 84 | 0 | 0 | 0 | 0 | 2 | 0 |
SpindlePole | 1 | 1 | 5 | 41 | 78 | 49 | 81 | 70 | – | 79 | 44 | 68 | 28 | 40 | 62 | 116 | 127 | 138 | 2 | 1 | 3 | 4 | 4 | 4 |
Vac/Vac Membrane | 0 | 0 | 0 | 1 | 6 | 2 | 17 | 24 | – | 17 | 9 | 7 | 2 | 3 | 4 | 6 | 14 | 16 | 0 | 0 | 3 | 11 | 16 | 25 |
Unique Cell Count | 42 | 64 | 125 | 324 | 458 | 471 | 640 | 776 | 616 | 462 | 658 | 310 | 437 | 554 | 378 | 413 | 430 | 90 | 97 | 137 | 149 | 345 | 420 | |
Labelled Cell Count | 47 | 80 | 159 | 446 | 619 | 663 | 869 | 984 | 827 | 658 | 930 | 371 | 494 | 657 | 526 | 624 | 642 | 90 | 97 | 137 | 149 | 345 | 420 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.2 | 7.1 | 5.2 | 5.0 | 4.6 | 4.1 | 3.4 | 3.6 | – | 3.3 | 3.4 | 3.3 | 6.8 | 6.8 | 6.2 | 8.0 | 8.0 | 8.2 | 7.5 | 7.6 | 7.7 |
Std Deviation (1e-4) | 1.1 | 0.9 | 0.7 | 1.0 | 1.3 | 1.3 | 1.0 | 1.1 | – | 0.6 | 1.0 | 0.6 | 0.8 | 1.0 | 1.0 | 1.2 | 1.2 | 1.3 | 1.4 | 1.2 | 1.3 |
Intensity Change (Log2) | – | – | – | -0.04 | -0.15 | -0.34 | -0.6 | -0.53 | – | -0.65 | -0.61 | -0.66 | 0.39 | 0.39 | 0.27 | 0.63 | 0.63 | 0.67 | 0.53 | 0.55 | 0.58 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -3.0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | -1.2 | -3.4 | -3.1 | -0.2 | 0.2 | -0.4 |
Bud | -0.5 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | -2.7 | -4.8 | -4.5 | -2.5 | -1.1 | -1.4 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 2.5 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0.7 | 0.8 | 0 |
Cell Periphery | 1.1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1.0 | 1.3 | 0.7 | 1.2 | 1.5 |
Cytoplasm | -1.3 | -4.4 | -2.5 | -3.2 | -3.8 | – | -3.5 | -6.6 | -6.5 | 2.2 | 3.4 | 3.3 | -5.4 | -6.6 | -6.3 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 2.9 | 4.0 | 3.4 |
Golgi | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | -1.0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | -1.9 | -3.4 | -3.7 |
Nucleus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 3.6 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 4.1 | 4.8 | 4.9 |
SpindlePole | 2.7 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1.8 | 1.9 | 2.4 | 6.1 | 6.1 | 6.3 |
Vacuole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.7588 | 1.9947 | 2.0054 | 1.7135 | 1.7751 | 1.971 | 1.1371 | 1.2996 | 1.4967 | 1.2666 | 1.3561 | 1.8708 | 0.1262 | 0.121 | 0.2501 | 0.3129 | 0.388 | 0.2096 |
Actin | 0.0376 | 0.0052 | 0.0241 | 0.0183 | 0.0175 | 0.0192 | 0.0593 | 0.0013 | 0.0621 | 0.0193 | 0.0268 | 0.0315 | 0.088 | 0.0153 | 0.0859 | 0.1479 | 0.022 | 0.0048 |
Bud | 0.0163 | 0.0151 | 0.0681 | 0.0362 | 0.0198 | 0.0054 | 0.0125 | 0.01 | 0.0629 | 0.0224 | 0.038 | 0.0035 | 0.0031 | 0.0079 | 0.0715 | 0.001 | 0.0044 | 0.0004 |
Bud Neck | 0.0407 | 0.0038 | 0.0285 | 0.0409 | 0.06 | 0.3822 | 0.0709 | 0.0044 | 0.0094 | 0.0164 | 0.007 | 0.4129 | 0.0233 | 0.0156 | 0.044 | 0.0044 | 0.0014 | 0.6221 |
Bud Periphery | 0.0441 | 0.0203 | 0.209 | 0.1534 | 0.0916 | 0.0094 | 0.0214 | 0.0212 | 0.1925 | 0.1338 | 0.1647 | 0.015 | 0.0054 | 0.0113 | 0.18 | 0.0038 | 0.0484 | 0.0006 |
Bud Site | 0.0841 | 0.1702 | 0.0754 | 0.0181 | 0.0107 | 0.0092 | 0.0903 | 0.1678 | 0.0623 | 0.0062 | 0.0074 | 0.0048 | 0.1546 | 0.2199 | 0.0598 | 0.003 | 0.0016 | 0.0005 |
Cell Periphery | 0.0082 | 0.0043 | 0.0053 | 0.0075 | 0.0101 | 0.0018 | 0.0107 | 0.0042 | 0.007 | 0.0107 | 0.0145 | 0.0028 | 0.017 | 0.0052 | 0.0066 | 0.0022 | 0.0156 | 0.0009 |
Cytoplasm | 0.5186 | 0.5869 | 0.3698 | 0.548 | 0.6088 | 0.4089 | 0.5231 | 0.6028 | 0.4187 | 0.5837 | 0.5929 | 0.3722 | 0.4168 | 0.5601 | 0.4249 | 0.4284 | 0.7481 | 0.1976 |
Cytoplasmic Foci | 0.0313 | 0.0189 | 0.0164 | 0.0085 | 0.0151 | 0.0255 | 0.0204 | 0.0144 | 0.0079 | 0.0447 | 0.0219 | 0.0234 | 0.0532 | 0.0291 | 0.0253 | 0.0979 | 0.0027 | 0.0617 |
Eisosomes | 0.0006 | 0.0001 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0028 | 0.0001 | 0.0005 | 0.0001 | 0.0009 | 0.0004 | 0.003 | 0.0002 | 0.001 | 0.0006 | 0.0008 | 0.0001 |
Endoplasmic Reticulum | 0.0077 | 0.0026 | 0.0037 | 0.0049 | 0.003 | 0.0023 | 0.009 | 0.0037 | 0.0031 | 0.0022 | 0.0048 | 0.0014 | 0.0131 | 0.0085 | 0.0027 | 0.0223 | 0.0242 | 0.0075 |
Endosome | 0.0134 | 0.0051 | 0.0064 | 0.0072 | 0.0039 | 0.0051 | 0.0105 | 0.0031 | 0.0033 | 0.0182 | 0.0093 | 0.0042 | 0.0168 | 0.0026 | 0.0054 | 0.0171 | 0.0017 | 0.013 |
Golgi | 0.0044 | 0.0011 | 0.0019 | 0.0019 | 0.0027 | 0.0012 | 0.0072 | 0.0006 | 0.0022 | 0.0036 | 0.0014 | 0.0029 | 0.0147 | 0.0011 | 0.0107 | 0.0117 | 0.0012 | 0.0024 |
Lipid Particles | 0.0017 | 0.0003 | 0.0009 | 0.0026 | 0.0003 | 0.0005 | 0.0064 | 0.0002 | 0.0007 | 0.0028 | 0.0009 | 0.0029 | 0.0207 | 0.0008 | 0.0016 | 0.0276 | 0.0007 | 0.0008 |
Mitochondria | 0.0031 | 0.0006 | 0.0023 | 0.0026 | 0.0014 | 0.0007 | 0.0015 | 0.0003 | 0.0014 | 0.0021 | 0.0011 | 0.001 | 0.0287 | 0.0003 | 0.0016 | 0.0018 | 0.0005 | 0.0011 |
None | 0.168 | 0.1485 | 0.1609 | 0.0997 | 0.1429 | 0.1166 | 0.134 | 0.1557 | 0.1549 | 0.087 | 0.0776 | 0.1091 | 0.1041 | 0.1166 | 0.0592 | 0.1411 | 0.1206 | 0.0767 |
Nuclear Periphery | 0.0041 | 0.0008 | 0.0041 | 0.0074 | 0.0007 | 0.0011 | 0.002 | 0.0005 | 0.0007 | 0.0006 | 0.001 | 0.0012 | 0.0055 | 0.0006 | 0.0005 | 0.0089 | 0.002 | 0.0027 |
Nucleolus | 0.001 | 0.001 | 0.002 | 0.0015 | 0.0006 | 0.0006 | 0.0009 | 0.0008 | 0.0002 | 0.0006 | 0.0017 | 0.0002 | 0.0007 | 0.0002 | 0.0004 | 0.0002 | 0.0001 | 0.0004 |
Nucleus | 0.0027 | 0.0042 | 0.0055 | 0.0068 | 0.0025 | 0.0017 | 0.0013 | 0.0014 | 0.001 | 0.0021 | 0.0019 | 0.0008 | 0.0012 | 0.001 | 0.001 | 0.0014 | 0.0007 | 0.001 |
Peroxisomes | 0.0022 | 0.0019 | 0.0017 | 0.0184 | 0.0006 | 0.0019 | 0.0071 | 0.0001 | 0.0046 | 0.0317 | 0.0007 | 0.0059 | 0.0236 | 0.0011 | 0.0143 | 0.0732 | 0 | 0.0003 |
Punctate Nuclear | 0.0043 | 0.0041 | 0.0052 | 0.0048 | 0.0026 | 0.0021 | 0.0059 | 0.0051 | 0.0006 | 0.0015 | 0.0206 | 0.0023 | 0.0018 | 0.0007 | 0.0005 | 0.0019 | 0.0002 | 0.0043 |
Vacuole | 0.0051 | 0.0045 | 0.0076 | 0.0101 | 0.0045 | 0.0041 | 0.0026 | 0.0021 | 0.0035 | 0.0094 | 0.0044 | 0.0015 | 0.0038 | 0.0017 | 0.0027 | 0.0025 | 0.0025 | 0.0008 |
Vacuole Periphery | 0.0007 | 0.0004 | 0.001 | 0.0011 | 0.0004 | 0.0004 | 0.0003 | 0.0002 | 0.0005 | 0.0008 | 0.0005 | 0.0004 | 0.0011 | 0.0001 | 0.0004 | 0.001 | 0.0003 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 25.2096 | 22.5358 | 7.5037 | 11.484 | 23.0474 | 18.7352 | 22.0556 | 18.7308 | 11.7328 | 24.6747 |
Translational Efficiency | 0.8797 | 0.8042 | 0.9928 | 0.5282 | 0.5676 | 0.8931 | 0.6806 | 0.5012 | 0.5935 | 0.547 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1341 | 1420 | 2638 | 146 | 797 | 166 | 1926 | 215 | 2138 | 1586 | 4564 | 361 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 752.78 | 786.75 | 915.48 | 951.68 | 731.53 | 842.06 | 790.52 | 933.33 | 744.86 | 792.54 | 862.75 | 940.75 |
Standard Deviation | 93.17 | 112.47 | 100.65 | 113.28 | 85.79 | 109.40 | 120.31 | 133.16 | 91.07 | 113.43 | 125.59 | 125.82 |
Intensity Change Log 2 | — | 0.063677 | 0.282300 | 0.338248 | — | 0.203006 | 0.111885 | 0.351470 | — | 0.134026 | 0.200825 | 0.344780 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.001268 | 0.003472 | 0.002927 | 0.003128 | 0.002249 | 0.011398 | 0.003378 | 0.018391 | 0.001633 | 0.004302 | 0.003118 | 0.012218 |
Bud Neck | 0.100504 | 0.104666 | 0.084716 | 0.047924 | 0.147605 | 0.140712 | 0.055779 | 0.072067 | 0.118063 | 0.108438 | 0.072505 | 0.062302 |
Bud Site | 0.245929* | 0.250993* | 0.245684* | 0.254853* | 0.168574* | 0.200930* | 0.250442* | 0.345979* | 0.217093* | 0.245753* | 0.247692* | 0.309125* |
Cell Periphery | 0.001345 | 0.000584 | 0.000469 | 0.000319 | 0.001014 | 0.002375 | 0.000224 | 0.001069 | 0.001222 | 0.000772 | 0.000366 | 0.000766 |
Cytoplasm | 0.445556* | 0.398601* | 0.504512* | 0.345118* | 0.374662* | 0.354384* | 0.546568* | 0.268888* | 0.419128* | 0.393973* | 0.522259* | 0.299718* |
Cytoplasmic Foci | 0.112750 | 0.123781 | 0.029330 | 0.070174 | 0.151396 | 0.136607 | 0.045532 | 0.075407 | 0.127156 | 0.125123 | 0.036167 | 0.073291 |
Eisosomes | 0.000423 | 0.000311 | 0.000091 | 0.000115 | 0.000666 | 0.002140 | 0.000056 | 0.000232 | 0.000513 | 0.000503 | 0.000076 | 0.000185 |
Endoplasmic Reticulum | 0.001686 | 0.000716 | 0.000846 | 0.001339 | 0.000901 | 0.000891 | 0.000369 | 0.000773 | 0.001393 | 0.000734 | 0.000645 | 0.001002 |
Endosome | 0.011547 | 0.021923 | 0.002206 | 0.006679 | 0.026342 | 0.026145 | 0.004517 | 0.016470 | 0.017062 | 0.022364 | 0.003182 | 0.012510 |
Golgi | 0.005463 | 0.008485 | 0.000379 | 0.000552 | 0.006345 | 0.018095 | 0.000995 | 0.009042 | 0.005792 | 0.009491 | 0.000639 | 0.005608 |
Lipid Particles | 0.002210 | 0.002658 | 0.000410 | 0.001610 | 0.003901 | 0.005422 | 0.000364 | 0.001396 | 0.002840 | 0.002947 | 0.000391 | 0.001483 |
Mitochondria | 0.001696 | 0.003154 | 0.000305 | 0.000104 | 0.003950 | 0.007940 | 0.000268 | 0.000193 | 0.002536 | 0.003655 | 0.000289 | 0.000157 |
Mitotic Spindle | 0.000159 | 0.000745 | 0.002361 | 0.001813 | 0.002009 | 0.007557 | 0.001941 | 0.003025 | 0.000848 | 0.001458 | 0.002184 | 0.002535 |
None | 0.001782 | 0.000456 | 0.000642 | 0.002006 | 0.000238 | 0.000073 | 0.000689 | 0.000094 | 0.001206 | 0.000416 | 0.000662 | 0.000868 |
Nuclear Periphery | 0.000038 | 0.000043 | 0.000084 | 0.000166 | 0.000100 | 0.000121 | 0.000042 | 0.000047 | 0.000061 | 0.000051 | 0.000066 | 0.000095 |
Nuclear Periphery Foci | 0.000955 | 0.000401 | 0.000532 | 0.003410 | 0.000414 | 0.001168 | 0.000507 | 0.000548 | 0.000753 | 0.000482 | 0.000522 | 0.001706 |
Nucleolus | 0.000444 | 0.000627 | 0.000115 | 0.000191 | 0.000263 | 0.000211 | 0.000137 | 0.000093 | 0.000376 | 0.000583 | 0.000124 | 0.000133 |
Nucleus | 0.015106 | 0.009493 | 0.043887 | 0.024728 | 0.018151 | 0.009353 | 0.020687 | 0.010139 | 0.016241 | 0.009479 | 0.034097 | 0.016039 |
Peroxisomes | 0.003472 | 0.008919 | 0.002370 | 0.012165 | 0.011847 | 0.014929 | 0.008027 | 0.022495 | 0.006594 | 0.009548 | 0.004757 | 0.018317 |
Vacuole | 0.046150 | 0.058206 | 0.077930 | 0.223386* | 0.077995 | 0.057531 | 0.059250 | 0.153383 | 0.058021 | 0.058136 | 0.070047 | 0.181695 |
Vacuole Periphery | 0.001517 | 0.001766 | 0.000203 | 0.000221 | 0.001378 | 0.002018 | 0.000228 | 0.000267 | 0.001465 | 0.001792 | 0.000214 | 0.000248 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.57 | -3.28 | -1.81 | 0.79 | 0.23 | -2.33 | -1.53 | -2.16 | -0.59 | -2.00 | -3.03 | -3.60 | -2.35 | -1.60 | -1.99 |
Bud Neck | -0.50 | 4.11 | 7.69 | 8.24 | 5.51 | 0.35 | 10.72 | 7.27 | 4.08 | -1.45 | 1.43 | 10.37 | 9.31 | 7.72 | 2.32 |
Bud Site | -0.39 | 2.74 | 2.54 | 2.73 | 1.49 | -1.61 | -5.78 | -5.93 | -3.64 | -3.34 | -3.04 | -0.87 | -2.21 | -0.55 | -1.90 |
Cell Periphery | 5.40 | 6.60 | 7.06 | 3.71 | 2.03 | -2.24 | 4.54 | 0.22 | 2.14 | -2.10 | 3.49 | 8.12 | 2.40 | 0.46 | -1.60 |
Cytoplasm | 4.03 | -6.17 | 3.84 | 1.97 | 6.64 | 0.90 | -14.42 | 4.16 | 2.29 | 13.57 | 2.54 | -13.47 | 6.69 | 4.94 | 14.19 |
Cytoplasmic Foci | -1.96 | 19.65 | 5.23 | 6.58 | -5.75 | 1.07 | 16.71 | 7.59 | 4.14 | -3.58 | 0.35 | 25.31 | 8.24 | 7.59 | -6.57 |
Eisosomes | 1.62 | 5.16 | 4.91 | 7.60 | -1.14 | -2.02 | 8.09 | 5.39 | 2.65 | -4.41 | 0.12 | 8.87 | 6.36 | 3.99 | -4.25 |
Endoplasmic Reticulum | 3.54 | 2.70 | 1.33 | -1.49 | -0.73 | 0.01 | 3.71 | 0.75 | 0.49 | -1.35 | 3.55 | 3.70 | 2.04 | -0.93 | -0.96 |
Endosome | -6.30 | 10.58 | 4.14 | 9.20 | -3.80 | 0.01 | 10.68 | 3.22 | 2.47 | -3.40 | -3.28 | 14.53 | 2.84 | 4.93 | -4.35 |
Golgi | -3.54 | 11.26 | 10.89 | 11.17 | -1.18 | -2.23 | 7.10 | -0.33 | 1.62 | -1.75 | -3.95 | 12.90 | 0.47 | 1.96 | -1.80 |
Lipid Particles | -0.99 | 8.85 | 1.52 | 1.97 | -2.48 | -0.65 | 6.06 | 2.70 | 1.63 | -1.44 | -0.22 | 9.64 | 2.65 | 2.34 | -2.23 |
Mitochondria | -1.95 | 4.39 | 5.02 | 4.52 | 6.34 | -0.84 | 3.25 | 3.32 | 1.68 | 1.21 | -1.24 | 4.81 | 5.10 | 4.53 | 3.07 |
Mitotic Spindle | -2.12 | -4.27 | -2.02 | -1.61 | -0.38 | -0.95 | -0.17 | -0.42 | 0.72 | -0.37 | -0.84 | -3.30 | -1.45 | -0.99 | -0.44 |
None | 3.73 | 3.28 | 0.18 | -1.04 | -0.86 | 3.89 | -5.15 | 2.71 | -0.97 | 7.06 | 3.37 | 2.50 | 1.04 | -0.64 | -0.09 |
Nuclear Periphery | -0.53 | -10.87 | -6.79 | -6.44 | -3.88 | -0.27 | 1.58 | 1.39 | 0.76 | -0.64 | 0.63 | -2.78 | -4.09 | -4.58 | -2.75 |
Nuclear Periphery Foci | 2.72 | 1.87 | -2.44 | -3.08 | -2.85 | -1.51 | -2.08 | -2.29 | 1.05 | -0.66 | 1.71 | 1.30 | -2.37 | -3.06 | -2.86 |
Nucleolus | -0.47 | 1.63 | 1.06 | 1.16 | -1.51 | 0.42 | 2.11 | 3.00 | 1.27 | 2.87 | -0.64 | 1.96 | 1.81 | 1.43 | -0.44 |
Nucleus | 4.26 | -18.53 | -2.76 | -4.27 | 6.39 | 3.20 | -4.13 | 3.21 | -0.73 | 6.73 | 6.24 | -17.77 | -0.55 | -3.98 | 10.91 |
Peroxisomes | -4.71 | 2.06 | -1.39 | -0.22 | -1.66 | -0.66 | 2.17 | -1.58 | -0.75 | -2.62 | -2.35 | 2.50 | -2.69 | -1.76 | -3.38 |
Vacuole | -3.80 | -14.62 | -11.21 | -10.65 | -8.90 | 2.68 | 0.37 | -7.05 | -7.94 | -7.28 | -0.16 | -10.69 | -13.21 | -13.13 | -10.90 |
Vacuole Periphery | -1.15 | 8.81 | 8.90 | 10.04 | -0.02 | -1.00 | 9.07 | 8.06 | 2.79 | -0.34 | -1.75 | 11.98 | 11.29 | 9.94 | -0.40 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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