Standard name
Human Ortholog
Description Protein of unknown function; may be involved in positive regulation of 1,3-beta-glucan synthesis and the Pkc1p-MAPK pathway; multicopy suppressor of temperature-sensitive mutations in CDC24 and CDC42, and of mutations in BEM4; potential Cdc28p substrate; relocalizes from bud neck to cytoplasm upon DNA replication stress
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.05 0.34 0.17 0.07 0 0 0 0 – 0 0 0 0.13 0.07 0.08 0.16 0.18 0.15 0.13 0.12 0.18 0.1 0.15 0.15
Bud 0 0.16 0.14 0.13 0.16 0.13 0.13 0.1 – 0.1 0.12 0.11 0.06 0 0 0.07 0.11 0.1 0.1 0.19 0.09 0.11 0.2 0.18
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.12 0.22 0.11 0.16 0.19 0.12
Bud Site 0.05 0 0 0.1 0.09 0.11 0.05 0 – 0.05 0.08 0.07 0 0 0 0.05 0.05 0 – – – – – –
Cell Periphery 0 0 0 0.06 0 0 0 0 – 0 0 0 0 0.05 0.06 0.05 0.06 0.07 0 0 0 0 0 0
Cytoplasm 0.76 0.55 0.66 0.6 0.44 0.54 0.51 0.48 – 0.49 0.34 0.35 0.77 0.81 0.8 0.39 0.33 0.35 0.48 0.38 0.44 0.36 0.28 0.36
Endoplasmic Reticulum 0 0.06 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.07 0 0 0 0.1 0 0 0 – 0 0 0 0 0 0 0.08 0.13 0.1 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.12 0 0.12 0.09 0.17 0.32 0.41 0.45 – 0.44 0.67 0.6 0 0 0 0.07 0 0 0 0 0.05 0.07 0 0
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0.13 0.12 0.06 0 0 – 0 0 0.08 0 0 0 0.15 0.19 0.2 0 0 0 0 0 0
SpindlePole 0 0 0 0.13 0.17 0.1 0.13 0.09 – 0.13 0.1 0.1 0.09 0.09 0.11 0.31 0.31 0.32 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.05 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 22 21 24 9 19 1 4 – 3 5 11 39 30 43 61 73 66 11 11 24 14 50 64
Bud 0 10 18 41 75 61 83 74 – 60 56 72 20 14 22 27 45 43 9 18 12 17 68 77
Bud Neck 0 0 2 2 3 8 12 3 – 10 4 4 6 2 8 1 15 4 10 21 15 24 66 51
Bud Site 2 2 4 34 42 53 32 18 – 28 35 45 4 3 3 18 20 17 – – – – – –
Cell Periphery 1 2 4 19 5 8 9 2 – 11 10 17 12 24 34 18 25 30 1 0 2 2 5 8
Cytoplasm 32 35 83 193 203 254 324 372 – 304 156 232 238 353 443 146 138 150 43 36 60 54 95 151
Endoplasmic Reticulum 1 4 3 4 5 12 4 2 – 1 2 10 1 1 7 9 7 13 1 0 3 2 10 9
Endosome 3 2 1 14 47 5 8 15 – 4 1 6 9 8 10 31 54 44 3 2 2 4 5 7
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 1 0 7 5 14 0 0 0 0 0 1
Mitochondria 5 0 15 29 79 151 260 347 – 274 309 394 0 0 0 25 16 15 2 3 7 9 14 10
Nucleus 0 0 1 1 6 4 4 8 – 4 5 6 1 0 2 0 1 2 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 2 0 1 2 – 3 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 1 6 7 9 17 – 8 5 6 0 0 0 3 6 6 0 0 0 0 0 0
Peroxisomes 0 2 2 42 53 30 24 26 – 21 17 51 11 15 19 58 78 84 0 0 0 0 2 0
SpindlePole 1 1 5 41 78 49 81 70 – 79 44 68 28 40 62 116 127 138 2 1 3 4 4 4
Vac/Vac Membrane 0 0 0 1 6 2 17 24 – 17 9 7 2 3 4 6 14 16 0 0 3 11 16 25
Unique Cell Count 42 64 125 324 458 471 640 776 616 462 658 310 437 554 378 413 430 90 97 137 149 345 420
Labelled Cell Count 47 80 159 446 619 663 869 984 827 658 930 371 494 657 526 624 642 90 97 137 149 345 420


Bud Neck, Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.2 7.1 5.2 5.0 4.6 4.1 3.4 3.6 – 3.3 3.4 3.3 6.8 6.8 6.2 8.0 8.0 8.2 7.5 7.6 7.7
Std Deviation (1e-4) 1.1 0.9 0.7 1.0 1.3 1.3 1.0 1.1 – 0.6 1.0 0.6 0.8 1.0 1.0 1.2 1.2 1.3 1.4 1.2 1.3
Intensity Change (Log2) – – – -0.04 -0.15 -0.34 -0.6 -0.53 – -0.65 -0.61 -0.66 0.39 0.39 0.27 0.63 0.63 0.67 0.53 0.55 0.58


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -3.0 0 0 0 0 – 0 0 0 -1.2 -3.4 -3.1 -0.2 0.2 -0.4
Bud -0.5 0 0 0 0 – 0 0 0 -2.7 -4.8 -4.5 -2.5 -1.1 -1.4
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 2.5 0 0 0 0 – 0 0 0 0 0 0 0.7 0.8 0
Cell Periphery 1.1 0 0 0 0 – 0 0 0 0 1.0 1.3 0.7 1.2 1.5
Cytoplasm -1.3 -4.4 -2.5 -3.2 -3.8 – -3.5 -6.6 -6.5 2.2 3.4 3.3 -5.4 -6.6 -6.3
Endoplasmic Reticulum 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 – 0 0 0 0 0 0 2.9 4.0 3.4
Golgi 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Mitochondria -1.0 0 0 0 0 – 0 0 0 0 0 0 -1.9 -3.4 -3.7
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Peroxisomes 3.6 0 0 0 0 – 0 0 0 0 0 0 4.1 4.8 4.9
SpindlePole 2.7 0 0 0 0 – 0 0 0 1.8 1.9 2.4 6.1 6.1 6.3
Vacuole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.7588 1.9947 2.0054 1.7135 1.7751 1.971 1.1371 1.2996 1.4967 1.2666 1.3561 1.8708 0.1262 0.121 0.2501 0.3129 0.388 0.2096
Actin 0.0376 0.0052 0.0241 0.0183 0.0175 0.0192 0.0593 0.0013 0.0621 0.0193 0.0268 0.0315 0.088 0.0153 0.0859 0.1479 0.022 0.0048
Bud 0.0163 0.0151 0.0681 0.0362 0.0198 0.0054 0.0125 0.01 0.0629 0.0224 0.038 0.0035 0.0031 0.0079 0.0715 0.001 0.0044 0.0004
Bud Neck 0.0407 0.0038 0.0285 0.0409 0.06 0.3822 0.0709 0.0044 0.0094 0.0164 0.007 0.4129 0.0233 0.0156 0.044 0.0044 0.0014 0.6221
Bud Periphery 0.0441 0.0203 0.209 0.1534 0.0916 0.0094 0.0214 0.0212 0.1925 0.1338 0.1647 0.015 0.0054 0.0113 0.18 0.0038 0.0484 0.0006
Bud Site 0.0841 0.1702 0.0754 0.0181 0.0107 0.0092 0.0903 0.1678 0.0623 0.0062 0.0074 0.0048 0.1546 0.2199 0.0598 0.003 0.0016 0.0005
Cell Periphery 0.0082 0.0043 0.0053 0.0075 0.0101 0.0018 0.0107 0.0042 0.007 0.0107 0.0145 0.0028 0.017 0.0052 0.0066 0.0022 0.0156 0.0009
Cytoplasm 0.5186 0.5869 0.3698 0.548 0.6088 0.4089 0.5231 0.6028 0.4187 0.5837 0.5929 0.3722 0.4168 0.5601 0.4249 0.4284 0.7481 0.1976
Cytoplasmic Foci 0.0313 0.0189 0.0164 0.0085 0.0151 0.0255 0.0204 0.0144 0.0079 0.0447 0.0219 0.0234 0.0532 0.0291 0.0253 0.0979 0.0027 0.0617
Eisosomes 0.0006 0.0001 0.0002 0.0002 0.0002 0.0001 0.0028 0.0001 0.0005 0.0001 0.0009 0.0004 0.003 0.0002 0.001 0.0006 0.0008 0.0001
Endoplasmic Reticulum 0.0077 0.0026 0.0037 0.0049 0.003 0.0023 0.009 0.0037 0.0031 0.0022 0.0048 0.0014 0.0131 0.0085 0.0027 0.0223 0.0242 0.0075
Endosome 0.0134 0.0051 0.0064 0.0072 0.0039 0.0051 0.0105 0.0031 0.0033 0.0182 0.0093 0.0042 0.0168 0.0026 0.0054 0.0171 0.0017 0.013
Golgi 0.0044 0.0011 0.0019 0.0019 0.0027 0.0012 0.0072 0.0006 0.0022 0.0036 0.0014 0.0029 0.0147 0.0011 0.0107 0.0117 0.0012 0.0024
Lipid Particles 0.0017 0.0003 0.0009 0.0026 0.0003 0.0005 0.0064 0.0002 0.0007 0.0028 0.0009 0.0029 0.0207 0.0008 0.0016 0.0276 0.0007 0.0008
Mitochondria 0.0031 0.0006 0.0023 0.0026 0.0014 0.0007 0.0015 0.0003 0.0014 0.0021 0.0011 0.001 0.0287 0.0003 0.0016 0.0018 0.0005 0.0011
None 0.168 0.1485 0.1609 0.0997 0.1429 0.1166 0.134 0.1557 0.1549 0.087 0.0776 0.1091 0.1041 0.1166 0.0592 0.1411 0.1206 0.0767
Nuclear Periphery 0.0041 0.0008 0.0041 0.0074 0.0007 0.0011 0.002 0.0005 0.0007 0.0006 0.001 0.0012 0.0055 0.0006 0.0005 0.0089 0.002 0.0027
Nucleolus 0.001 0.001 0.002 0.0015 0.0006 0.0006 0.0009 0.0008 0.0002 0.0006 0.0017 0.0002 0.0007 0.0002 0.0004 0.0002 0.0001 0.0004
Nucleus 0.0027 0.0042 0.0055 0.0068 0.0025 0.0017 0.0013 0.0014 0.001 0.0021 0.0019 0.0008 0.0012 0.001 0.001 0.0014 0.0007 0.001
Peroxisomes 0.0022 0.0019 0.0017 0.0184 0.0006 0.0019 0.0071 0.0001 0.0046 0.0317 0.0007 0.0059 0.0236 0.0011 0.0143 0.0732 0 0.0003
Punctate Nuclear 0.0043 0.0041 0.0052 0.0048 0.0026 0.0021 0.0059 0.0051 0.0006 0.0015 0.0206 0.0023 0.0018 0.0007 0.0005 0.0019 0.0002 0.0043
Vacuole 0.0051 0.0045 0.0076 0.0101 0.0045 0.0041 0.0026 0.0021 0.0035 0.0094 0.0044 0.0015 0.0038 0.0017 0.0027 0.0025 0.0025 0.0008
Vacuole Periphery 0.0007 0.0004 0.001 0.0011 0.0004 0.0004 0.0003 0.0002 0.0005 0.0008 0.0005 0.0004 0.0011 0.0001 0.0004 0.001 0.0003 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 25.2096 22.5358 7.5037 11.484 23.0474 18.7352 22.0556 18.7308 11.7328 24.6747
Translational Efficiency 0.8797 0.8042 0.9928 0.5282 0.5676 0.8931 0.6806 0.5012 0.5935 0.547

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Protein of unknown function; may be involved in positive regulation of 1,3-beta-glucan synthesis and the Pkc1p-MAPK pathway; multicopy suppressor of temperature-sensitive mutations in CDC24 and CDC42, and of mutations in BEM4; potential Cdc28p substrate; relocalizes from bud neck to cytoplasm upon DNA replication stress
Localization
Cell Percentages cytoplasm (56%), bud neck (11%), mixed (28%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Msb1

Msb1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Msb1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available