Standard name | |
---|---|
Human Ortholog | |
Description | Ca2+-dependent cysteine protease; may cleave specific substrates during the stress response; regulates apoptosis upon H2O2 treatment; required for clearance of insoluble protein aggregates during normal growth; implicated in cell cycle dynamics and lifespan extension; undergoes autocatalytic processing; similar to mammalian metacaspases, but exists as a monomer due to an extra pair of anti-parallel beta-strands that block potential dimerization |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 3 | 4 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 6 | 4 | 8 | 4 | 6 | 11 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 4 | 8 | 9 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 2 | 3 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 2 | 2 | 3 | 1 | 9 | 5 | 12 | 9 | 5 | 7 | 0 | 2 | 2 | 1 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 270 | 284 | 386 | 208 | 252 | 379 | 436 | 357 | 374 | 430 | 404 | 376 | 419 | 632 | 611 | 143 | 202 | 177 | 258 | 256 | 382 | 349 | 365 | 402 |
Endoplasmic Reticulum | 1 | 2 | 5 | 2 | 4 | 11 | 15 | 9 | 9 | 17 | 3 | 8 | 2 | 6 | 3 | 3 | 4 | 5 | 3 | 0 | 1 | 1 | 4 | 6 |
Endosome | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 5 | 0 | 0 | 1 | 3 | 2 | 0 | 2 | 0 | 1 | 2 | 1 | 2 | 0 | 4 | 9 | 23 |
Golgi | 0 | 1 | 0 | 2 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 1 | 0 | 2 | 2 | 0 | 6 | 28 |
Mitochondria | 1 | 0 | 0 | 5 | 11 | 35 | 97 | 58 | 83 | 92 | 100 | 92 | 1 | 0 | 0 | 2 | 2 | 1 | 0 | 1 | 0 | 2 | 2 | 6 |
Nucleus | 3 | 0 | 5 | 1 | 4 | 9 | 18 | 15 | 18 | 16 | 15 | 10 | 3 | 16 | 9 | 2 | 1 | 3 | 3 | 9 | 4 | 24 | 29 | 24 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
Nucleolus | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 5 | 9 |
Vac/Vac Membrane | 1 | 5 | 18 | 64 | 44 | 36 | 110 | 129 | 115 | 76 | 105 | 122 | 39 | 33 | 72 | 21 | 15 | 16 | 0 | 6 | 3 | 4 | 16 | 17 |
Unique Cell Count | 272 | 291 | 401 | 265 | 304 | 444 | 616 | 529 | 558 | 593 | 568 | 566 | 452 | 645 | 654 | 158 | 212 | 192 | 274 | 288 | 400 | 396 | 462 | 548 |
Labelled Cell Count | 276 | 293 | 417 | 285 | 320 | 471 | 692 | 587 | 620 | 644 | 640 | 630 | 467 | 689 | 699 | 178 | 228 | 207 | 274 | 288 | 400 | 396 | 462 | 548 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 10.7 | 10.9 | 10.7 | 9.4 | 9.4 | 8.5 | 8.3 | 8.7 | 8.2 | 7.9 | 8.0 | 7.8 | 12.4 | 12.1 | 11.4 | 13.6 | 12.7 | 13.2 | 12.4 | 12.3 | 14.1 |
Std Deviation (1e-4) | 1.8 | 2.4 | 1.9 | 1.7 | 1.8 | 1.8 | 1.8 | 1.9 | 1.8 | 1.7 | 1.8 | 1.8 | 2.3 | 2.0 | 2.0 | 3.6 | 2.9 | 3.0 | 2.5 | 2.5 | 2.8 |
Intensity Change (Log2) | – | – | – | -0.18 | -0.18 | -0.33 | -0.37 | -0.3 | -0.39 | -0.44 | -0.42 | -0.45 | 0.21 | 0.17 | 0.09 | 0.34 | 0.25 | 0.3 | 0.22 | 0.2 | 0.4 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 2.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -7.2 | -6.0 | -5.4 | -10.1 | -10.8 | -11.0 | -9.6 | -9.9 | -11.2 | -2.3 | 1.7 | -2.0 | -2.7 | -0.6 | -2.1 |
Endoplasmic Reticulum | 0 | 0 | 1.3 | 1.3 | 0.6 | 0.5 | 1.7 | 0 | 0.2 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 5.7 | 8.4 | 6.8 | 8.1 | 8.3 | 8.9 | 8.5 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0.9 | 1.8 | 1.7 | 2.0 | 1.6 | 1.5 | 0.6 | 0 | 1.4 | 0.2 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 7.6 | 4.6 | 2.1 | 6.3 | 8.2 | 7.1 | 4.4 | 6.4 | 7.4 | 2.4 | 0.5 | 3.7 | 3.7 | 1.3 | 1.9 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 7.1099 | 7.5801 | 7.7622 | 6.6917 | 6.8739 | 7.6029 | 4.4077 | 5.8866 | 5.4204 | 5.0324 | 4.9291 | 5.642 | 5.2444 | 6.0483 | 5.5234 | 5.3784 | 5.0271 | 5.5186 |
Actin | 0.0292 | 0.0019 | 0.0093 | 0.0008 | 0.0359 | 0.0008 | 0.0454 | 0.0007 | 0.0134 | 0.0311 | 0.0044 | 0.0004 | 0.0094 | 0.0009 | 0.0108 | 0.0016 | 0.0289 | 0.0052 |
Bud | 0.0009 | 0.0012 | 0.0008 | 0.0001 | 0.0003 | 0.0001 | 0.0009 | 0.0004 | 0.0023 | 0.0006 | 0.0002 | 0.0003 | 0.0011 | 0.0033 | 0.0007 | 0.0002 | 0.0019 | 0.0009 |
Bud Neck | 0.009 | 0.0004 | 0.0008 | 0.0006 | 0.0005 | 0.0014 | 0.0134 | 0.0006 | 0.0131 | 0.0017 | 0.0005 | 0.0034 | 0.0065 | 0.0007 | 0.0014 | 0.0013 | 0.0067 | 0.0076 |
Bud Periphery | 0.001 | 0.0004 | 0.0005 | 0 | 0.0002 | 0 | 0.0009 | 0.0002 | 0.0011 | 0.0005 | 0.0003 | 0.0001 | 0.001 | 0.001 | 0.0003 | 0.0001 | 0.001 | 0.0008 |
Bud Site | 0.0033 | 0.0043 | 0.0051 | 0.0003 | 0.0004 | 0.0001 | 0.0118 | 0.0032 | 0.0081 | 0.0024 | 0.0002 | 0.0004 | 0.0059 | 0.0159 | 0.0057 | 0.0004 | 0.0021 | 0.0007 |
Cell Periphery | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0.0007 | 0.0001 | 0.0004 | 0.0001 | 0.0001 | 0 | 0.0004 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 |
Cytoplasm | 0.7426 | 0.906 | 0.8369 | 0.9554 | 0.8466 | 0.924 | 0.7667 | 0.9483 | 0.8811 | 0.7588 | 0.8412 | 0.9493 | 0.826 | 0.9014 | 0.867 | 0.83 | 0.794 | 0.8475 |
Cytoplasmic Foci | 0.0235 | 0.0158 | 0.0251 | 0.0025 | 0.0171 | 0.006 | 0.0162 | 0.002 | 0.0061 | 0.0271 | 0.0054 | 0.0039 | 0.0155 | 0.0047 | 0.0065 | 0.0124 | 0.0141 | 0.0072 |
Eisosomes | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0.0005 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0005 | 0 |
Endoplasmic Reticulum | 0.0127 | 0.0029 | 0.0031 | 0.003 | 0.0037 | 0.0026 | 0.014 | 0.0033 | 0.0023 | 0.0025 | 0.0021 | 0.0024 | 0.0071 | 0.0021 | 0.0023 | 0.0028 | 0.0092 | 0.003 |
Endosome | 0.0269 | 0.0038 | 0.0119 | 0.0012 | 0.0169 | 0.0019 | 0.0405 | 0.0006 | 0.0061 | 0.0492 | 0.0454 | 0.0024 | 0.0192 | 0.0042 | 0.0072 | 0.0027 | 0.0279 | 0.0059 |
Golgi | 0.0054 | 0.0015 | 0.0036 | 0.0001 | 0.0035 | 0.0001 | 0.01 | 0.0001 | 0.0083 | 0.0318 | 0.0081 | 0.0001 | 0.0029 | 0.0002 | 0.0023 | 0.0003 | 0.009 | 0.0005 |
Lipid Particles | 0.002 | 0.0002 | 0.0014 | 0 | 0.0144 | 0 | 0.004 | 0 | 0.0015 | 0.0031 | 0.0013 | 0 | 0.0052 | 0.0001 | 0.0007 | 0.0003 | 0.0107 | 0.0006 |
Mitochondria | 0.0012 | 0.0003 | 0.0023 | 0.0002 | 0.0006 | 0.0002 | 0.0041 | 0.0001 | 0.0114 | 0.0361 | 0.0549 | 0.0002 | 0.0036 | 0.0004 | 0.001 | 0.0003 | 0.0023 | 0.0005 |
None | 0.1117 | 0.038 | 0.0609 | 0.0144 | 0.0189 | 0.0366 | 0.028 | 0.0208 | 0.0082 | 0.0033 | 0.0193 | 0.0041 | 0.0186 | 0.0124 | 0.0144 | 0.0094 | 0.0121 | 0.0149 |
Nuclear Periphery | 0.0125 | 0.0022 | 0.0028 | 0.0022 | 0.0061 | 0.002 | 0.0127 | 0.0015 | 0.0018 | 0.0032 | 0.0009 | 0.0023 | 0.0102 | 0.0046 | 0.0038 | 0.0062 | 0.017 | 0.0084 |
Nucleolus | 0.0002 | 0.0001 | 0.0004 | 0 | 0.0001 | 0 | 0.001 | 0 | 0.0003 | 0.0001 | 0 | 0 | 0.0005 | 0.0003 | 0.0004 | 0.0002 | 0.0009 | 0.0005 |
Nucleus | 0.0089 | 0.0152 | 0.0235 | 0.0169 | 0.0091 | 0.0217 | 0.015 | 0.0161 | 0.0308 | 0.0374 | 0.009 | 0.0281 | 0.0524 | 0.0408 | 0.0679 | 0.1185 | 0.0366 | 0.0789 |
Peroxisomes | 0.0027 | 0.0022 | 0.0065 | 0.0001 | 0.0165 | 0.0003 | 0.0021 | 0.0001 | 0.0011 | 0.0069 | 0.0006 | 0.0001 | 0.0043 | 0.0001 | 0.0012 | 0.0006 | 0.0065 | 0.0004 |
Punctate Nuclear | 0.0039 | 0.0027 | 0.0034 | 0.0016 | 0.0077 | 0.0011 | 0.0039 | 0.0013 | 0.001 | 0.0022 | 0.0004 | 0.0017 | 0.0063 | 0.0054 | 0.005 | 0.0116 | 0.0146 | 0.0151 |
Vacuole | 0.0018 | 0.0007 | 0.0012 | 0.0005 | 0.001 | 0.0007 | 0.0062 | 0.0004 | 0.0009 | 0.0013 | 0.0035 | 0.0009 | 0.0028 | 0.0012 | 0.0009 | 0.0009 | 0.0026 | 0.0011 |
Vacuole Periphery | 0.0005 | 0.0001 | 0.0003 | 0.0001 | 0.0004 | 0.0001 | 0.0022 | 0.0001 | 0.0004 | 0.0006 | 0.0019 | 0.0001 | 0.001 | 0.0002 | 0.0002 | 0.0001 | 0.0011 | 0.0004 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 85.3673 | 66.8215 | 58.0363 | 91.8814 | 81.4975 | 72.1612 | 73.8972 | 89.6466 | 76.6839 | 98.2224 |
Translational Efficiency | 1.5431 | 1.541 | 1.7262 | 0.8616 | 1.2493 | 1.8303 | 1.3542 | 0.8073 | 1.1994 | 1.1271 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1544 | 235 | 226 | 1155 | 1631 | 1021 | 1056 | 158 | 3175 | 1256 | 1282 | 1313 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 970.55 | 1252.64 | 1545.12 | 1654.57 | 1008.86 | 1157.21 | 1461.69 | 1823.86 | 990.23 | 1175.07 | 1476.40 | 1674.94 |
Standard Deviation | 135.39 | 197.20 | 207.66 | 290.63 | 170.16 | 173.82 | 230.38 | 336.35 | 155.42 | 182.27 | 228.76 | 301.57 |
Intensity Change Log 2 | — | 0.368097 | 0.670844 | 0.769582 | — | 0.197925 | 0.534911 | 0.854269 | — | 0.283873 | 0.603163 | 0.813366 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000491 | 0.000988 | 0.001330 | 0.004769 | 0.000581 | 0.000480 | 0.000781 | 0.000712 | 0.000537 | 0.000575 | 0.000878 | 0.004281 |
Bud Neck | 0.001654 | 0.002441 | 0.005860 | 0.010616 | 0.001383 | 0.001669 | 0.003364 | 0.007759 | 0.001515 | 0.001813 | 0.003804 | 0.010272 |
Bud Site | 0.008605 | 0.012491 | 0.016675 | 0.020410 | 0.003705 | 0.010983 | 0.007966 | 0.027711 | 0.006088 | 0.011265 | 0.009501 | 0.021289 |
Cell Periphery | 0.000187 | 0.000174 | 0.000475 | 0.000573 | 0.000131 | 0.000088 | 0.000247 | 0.000374 | 0.000158 | 0.000104 | 0.000287 | 0.000549 |
Cytoplasm | 0.759804* | 0.655131* | 0.244638* | 0.318532* | 0.723910* | 0.741957* | 0.369314* | 0.105627* | 0.741366* | 0.725712* | 0.347335* | 0.292912* |
Cytoplasmic Foci | 0.061690 | 0.022131 | 0.009149 | 0.009821 | 0.038785 | 0.041211 | 0.008721 | 0.171705 | 0.049924 | 0.037641 | 0.008796 | 0.029301 |
Eisosomes | 0.000023 | 0.000016 | 0.000035 | 0.000024 | 0.000022 | 0.000016 | 0.000019 | 0.000029 | 0.000023 | 0.000016 | 0.000021 | 0.000024 |
Endoplasmic Reticulum | 0.002007 | 0.001237 | 0.003228 | 0.001358 | 0.002453 | 0.001120 | 0.002399 | 0.000913 | 0.002236 | 0.001142 | 0.002545 | 0.001304 |
Endosome | 0.001512 | 0.002213 | 0.002403 | 0.004913 | 0.001481 | 0.003155 | 0.003193 | 0.081524 | 0.001496 | 0.002979 | 0.003053 | 0.014132 |
Golgi | 0.000692 | 0.002620 | 0.000278 | 0.011984 | 0.000472 | 0.003736 | 0.003380 | 0.126550* | 0.000579 | 0.003527 | 0.002833 | 0.025770 |
Lipid Particles | 0.000938 | 0.000662 | 0.002404 | 0.003318 | 0.000790 | 0.001455 | 0.001653 | 0.002139 | 0.000862 | 0.001307 | 0.001785 | 0.003176 |
Mitochondria | 0.000248 | 0.000150 | 0.000788 | 0.020591 | 0.000859 | 0.001611 | 0.000958 | 0.014736 | 0.000562 | 0.001338 | 0.000928 | 0.019886 |
Mitotic Spindle | 0.000661 | 0.010098 | 0.007085 | 0.074110 | 0.002189 | 0.014974 | 0.023669 | 0.098278 | 0.001446 | 0.014061 | 0.020746 | 0.077018 |
None | 0.004386 | 0.011380 | 0.006366 | 0.009402 | 0.004630 | 0.006276 | 0.010357 | 0.008085 | 0.004511 | 0.007231 | 0.009654 | 0.009243 |
Nuclear Periphery | 0.000134 | 0.000307 | 0.001646 | 0.001456 | 0.000389 | 0.000198 | 0.001149 | 0.000765 | 0.000265 | 0.000218 | 0.001237 | 0.001372 |
Nuclear Periphery Foci | 0.004087 | 0.004004 | 0.006693 | 0.005842 | 0.004904 | 0.003084 | 0.005650 | 0.002704 | 0.004507 | 0.003257 | 0.005834 | 0.005464 |
Nucleolus | 0.000430 | 0.000117 | 0.000215 | 0.000902 | 0.000473 | 0.000192 | 0.000149 | 0.000275 | 0.000452 | 0.000178 | 0.000161 | 0.000827 |
Nucleus | 0.117995 | 0.218427* | 0.558647* | 0.419011* | 0.178189* | 0.125654 | 0.502919* | 0.042056 | 0.148917* | 0.143012* | 0.512743* | 0.373650* |
Peroxisomes | 0.000426 | 0.000364 | 0.000163 | 0.000761 | 0.000363 | 0.002713 | 0.000600 | 0.002421 | 0.000394 | 0.002273 | 0.000523 | 0.000961 |
Vacuole | 0.033574 | 0.053641 | 0.131764 | 0.066892 | 0.033969 | 0.037799 | 0.053123 | 0.149097 | 0.033777 | 0.040763 | 0.066986 | 0.076784 |
Vacuole Periphery | 0.000456 | 0.001406 | 0.000158 | 0.014716 | 0.000320 | 0.001630 | 0.000390 | 0.156541* | 0.000386 | 0.001588 | 0.000349 | 0.031782 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -1.06 | -5.24 | -2.81 | -2.12 | -1.01 | 0.24 | -1.66 | -0.03 | -0.67 | 3.86 | -0.17 | -3.52 | -2.74 | -2.79 | -1.69 |
Bud Neck | -1.17 | -9.12 | -13.62 | -11.16 | -2.18 | -1.44 | -11.33 | -7.64 | -7.14 | -2.54 | -1.60 | -14.17 | -14.84 | -14.06 | -7.29 |
Bud Site | -0.52 | -2.01 | -5.09 | -1.49 | -0.98 | -3.77 | -4.54 | -5.49 | -2.73 | -3.46 | -2.51 | -3.18 | -9.25 | -3.79 | -5.39 |
Cell Periphery | 0.31 | -6.37 | -12.24 | -10.72 | -0.51 | 4.12 | -12.62 | -4.96 | -5.73 | -0.88 | 3.93 | -11.79 | -14.28 | -15.91 | -6.82 |
Cytoplasm | 9.24 | 43.90 | 63.24* | 20.53 | -5.56 | 0.21 | 51.91 | 25.74 | 24.71 | 4.07 | 5.23 | 67.68 | 70.38 | 48.62 | 1.18 |
Cytoplasmic Foci | 10.59 | 20.26 | 16.64 | 1.43 | -4.89 | -0.91 | 17.30 | 1.13 | 1.48 | -3.60 | 4.57 | 26.90 | 15.59 | 7.56 | -7.62 |
Eisosomes | 1.19 | -8.13 | -4.83 | -4.88 | 5.00 | 1.86 | -4.01 | -1.32 | -3.30 | 2.24 | 2.79 | -7.16 | -5.20 | -7.95 | 1.40 |
Endoplasmic Reticulum | 1.82 | -4.54 | 2.39 | 0.28 | 7.50 | 3.52 | -1.27 | 5.36 | 3.80 | 12.82 | 4.15 | -3.37 | 4.35 | -0.20 | 11.66 |
Endosome | -0.72 | -3.36 | 0.83 | 1.07 | 3.90 | -2.67 | -0.38 | -1.45 | -0.85 | -1.39 | -2.78 | -0.95 | -0.81 | 1.77 | -0.00 |
Golgi | -0.62 | 2.47 | -1.01 | 0.21 | -1.67 | -3.11 | -0.98 | -1.35 | -0.68 | -1.24 | -3.07 | -0.66 | -1.81 | 1.07 | -1.32 |
Lipid Particles | 0.28 | -7.72 | -11.28 | -11.66 | -6.07 | -1.94 | -7.82 | -6.50 | -5.37 | -4.73 | -1.71 | -9.38 | -12.61 | -10.78 | -9.45 |
Mitochondria | 1.02 | -7.04 | -2.12 | -2.21 | -1.43 | -0.72 | -0.46 | -0.21 | 0.57 | 0.21 | -1.11 | -1.98 | -1.83 | -1.12 | -1.37 |
Mitotic Spindle | -2.06 | -3.58 | -12.21 | -8.77 | -9.60 | -3.83 | -4.88 | -6.56 | -6.10 | -6.03 | -4.50 | -5.81 | -13.53 | -10.77 | -10.66 |
None | -1.91 | -0.27 | -8.85 | -1.00 | -6.09 | -1.57 | -6.50 | -3.20 | -2.64 | -1.40 | -2.55 | -6.81 | -9.84 | -5.37 | -4.16 |
Nuclear Periphery | -8.13 | -18.04 | -24.74 | -20.76 | 0.65 | 1.17 | -11.66 | -8.62 | -11.38 | -0.85 | -0.29 | -21.36 | -21.41 | -24.73 | -4.75 |
Nuclear Periphery Foci | -0.55 | -1.49 | -4.62 | -3.23 | -2.02 | 2.06 | 0.54 | -1.12 | -1.61 | -1.27 | 2.01 | -0.05 | -3.97 | -5.44 | -3.57 |
Nucleolus | 2.15 | 0.92 | -1.72 | -6.80 | -4.54 | 1.35 | 1.08 | -0.13 | -2.78 | -2.29 | 2.23 | 1.61 | -1.94 | -6.47 | -5.62 |
Nucleus | -8.52 | -20.25 | -30.33 | -10.26 | 5.82 | 7.08 | -38.50 | 3.04 | 0.15 | 23.89 | -0.61 | -47.35 | -24.74 | -22.17 | 17.09 |
Peroxisomes | 0.62 | 2.13 | 0.97 | 0.10 | -1.42 | -2.09 | 0.87 | -0.97 | 1.69 | -1.46 | -2.04 | 1.53 | 0.37 | 2.09 | -1.27 |
Vacuole | -6.17 | -14.15 | -24.77 | -7.93 | 2.74 | -4.49 | -14.84 | -13.16 | -12.41 | -9.49 | -7.37 | -19.60 | -27.53 | -21.33 | -8.83 |
Vacuole Periphery | -0.49 | 1.32 | -1.08 | -0.44 | -1.47 | -1.91 | 1.47 | -1.26 | -1.06 | -1.29 | -1.87 | 1.89 | -1.73 | -0.66 | -1.97 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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