Standard name
Human Ortholog
Description Component of mRNP complexes associated with polyribosomes; involved in localization of mRNAs to P bodies; implicated in secretion and nuclear segregation; multicopy suppressor of BFA (Brefeldin A) sensitivity

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.06 0 0.05 0.29 0.11 0.18 0.1 0.1 0.08 0.16 0.15 0.21 0 0 0 0 0 0 0 0 0.06 0.07 0.09 0.06
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0.05 0 0 0.16 0 0.05 0 0.08 0.05 0.08 0.18 0.22 0.15 0.1 0.22 0.26 0.22 0 0 0 0 0.05 0
Cytoplasm 0.3 0.23 0.1 0 0.11 0.06 0.11 0.13 0.06 0.07 0.08 0.18 0.15 0.42 0.66 0.58 0.57 0.64 0.06 0.07 0 0 0 0.08
Endoplasmic Reticulum 0.7 0.78 0.85 0.53 0.37 0.61 0.65 0.68 0.69 0.72 0.54 0.51 0.69 0.55 0.35 0 0 0 0.82 0.85 0.73 0.79 0.72 0.65
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0.05 0 0.15 0.5 0.58 0.29 0.22 0.15 0.08 0.08 0.23 0.1 0 0 0 0.27 0.27 0.15 0 0 0.08 0.05 0.06 0.1
Mitochondria 0 0 0 0.38 0.21 0.37 0.17 0.14 0.28 0.38 0.27 0.36 0 0 0 0.06 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 10 3 1 10 2 9 11 14 3 12 7 8 9 3 1 2 0 1 8 1 3 11 27 16
Bud 3 1 0 0 0 1 3 1 1 1 0 1 4 0 0 1 0 2 1 0 0 0 4 5
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 2
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1 5 0 0 3 0 5 3 3 4 4 7 45 49 34 43 46 37 1 0 0 5 13 3
Cytoplasm 52 25 2 1 2 3 12 18 2 5 4 7 31 137 224 114 101 107 12 8 0 4 9 20
Endoplasmic Reticulum 121 84 17 18 7 31 71 94 25 55 26 20 143 181 119 8 6 6 165 99 44 140 214 170
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Golgi 9 4 3 17 11 15 24 21 3 6 11 4 9 10 0 53 47 25 8 3 5 8 18 26
Mitochondria 1 0 0 13 4 19 19 20 10 29 13 14 0 0 0 11 2 6 3 0 0 1 5 4
Nucleus 0 0 0 0 0 0 2 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 2 0 0 3 0 2 0 0 0 0 0 0 0 0 1 0 0 1
Nucleolus 1 0 0 0 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 1 0 4 2 0 0 0 0 0 0 1 0 0 0 0 1 0 0 3 5
Unique Cell Count 174 108 20 34 19 51 110 138 36 76 48 39 206 329 339 197 176 166 202 117 61 177 298 263
Labelled Cell Count 198 122 23 59 31 78 154 174 48 115 65 63 241 380 379 233 202 184 202 117 61 177 298 263


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 45.5 58.5 41.7 38.6 43.8 39.9 31.1 29.1 30.2 28.2 26.1 26.2 47.1 52.0 48.9 65.0 71.6 70.5 40.7 47.1 53.4
Std Deviation (1e-4) 7.7 8.5 12.1 11.0 10.3 9.2 8.6 10.0 6.0 6.2 5.5 8.0 11.8 9.0 8.8 21.6 23.3 21.6 11.7 13.3 17.7
Intensity Change (Log2) -0.11 0.07 -0.06 -0.42 -0.52 -0.47 -0.57 -0.67 -0.67 0.18 0.32 0.23 0.64 0.78 0.76 -0.04 0.18 0.36


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 2.8 5.0 4.1 4.0 4.7
Endoplasmic Reticulum -2.4 -3.1 -2.0 -1.8 -1.5 -1.3 -1.2 -2.4 -2.5 -1.5 -2.6 -4.5 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 2.6 2.8 1.3 0 0 0 0 0 0 0 0 0 1.2 1.1 0
Mitochondria 0 0 3.2 0 0 0 3.3 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 35.7379 48.1602 45.0492 44.9957 34.7788 43.5 37.8582 45.3261 43.0919 37.1088 35.8206 42.088 44.5134 46.3075 46.2898 43.7259 37.3335 44.2755
Actin 0.03 0.0195 0.0049 0.0005 0.0069 0.0005 0.044 0.0137 0.044 0.0068 0.0089 0.0051 0.0135 0.0256 0.0147 0.0141 0.0352 0.0114
Bud 0.001 0.0011 0.0003 0 0.0005 0 0.0008 0.0004 0.0003 0.0003 0.0003 0.0001 0.0004 0.0003 0.0001 0.0004 0.0049 0.0005
Bud Neck 0.0066 0.0009 0.0008 0.0004 0.0022 0.0004 0.0008 0.0009 0.002 0.0012 0.0008 0.0017 0.0006 0.0012 0.0005 0.004 0.0045 0.0019
Bud Periphery 0.0019 0.0034 0.0007 0.0003 0.0012 0.0001 0.0013 0.0011 0.001 0.0013 0.001 0.0003 0.0009 0.0005 0.0003 0.0018 0.014 0.0016
Bud Site 0.0111 0.0069 0.0003 0 0.0005 0 0.0028 0.0018 0.0006 0.0002 0.0001 0.0001 0.0006 0.0017 0.0002 0.0005 0.001 0.0003
Cell Periphery 0.0061 0.0194 0.0167 0.0086 0.0071 0.0022 0.0041 0.0175 0.0104 0.0164 0.0011 0.0033 0.0123 0.0168 0.007 0.0156 0.0126 0.0026
Cytoplasm 0.0385 0.0784 0.0434 0.0077 0.0118 0.022 0.0589 0.043 0.009 0.0104 0.0153 0.0174 0.0251 0.0521 0.028 0.05 0.0351 0.0248
Cytoplasmic Foci 0.031 0.0114 0.0102 0.0002 0.0603 0.0087 0.0077 0.0056 0.0042 0.0026 0.0031 0.0019 0.0084 0.0068 0.0041 0.0025 0.0058 0.0023
Eisosomes 0.0014 0.0022 0.0043 0.0006 0.0003 0.0004 0.0012 0.0032 0.0145 0.0143 0.0002 0.0011 0.0021 0.0079 0.0012 0.0059 0.0021 0.0011
Endoplasmic Reticulum 0.5374 0.677 0.7664 0.8685 0.6185 0.8169 0.6515 0.7383 0.6819 0.7877 0.6632 0.7563 0.6885 0.7011 0.8035 0.7747 0.5881 0.7583
Endosome 0.0408 0.0141 0.012 0.0075 0.0558 0.012 0.0302 0.0166 0.0185 0.0194 0.0341 0.024 0.019 0.0165 0.0132 0.0175 0.0267 0.0195
Golgi 0.0208 0.0617 0.0564 0.0623 0.0852 0.0634 0.0345 0.0943 0.1455 0.0591 0.1473 0.0903 0.0328 0.0983 0.0737 0.0752 0.135 0.0948
Lipid Particles 0.025 0.0304 0.0218 0.0003 0.0566 0.0051 0.0107 0.0162 0.0174 0.0044 0.0017 0.0075 0.0121 0.032 0.02 0.0053 0.0129 0.0048
Mitochondria 0.0335 0.0107 0.0187 0.0018 0.025 0.0044 0.0126 0.0124 0.005 0.032 0.0564 0.0086 0.0063 0.006 0.0094 0.0046 0.04 0.0066
None 0.0049 0.0005 0.0006 0.0001 0.0004 0.0001 0.0129 0.0008 0.0022 0.0006 0.0005 0.0003 0.0021 0.0003 0.0003 0.0004 0.0046 0.0006
Nuclear Periphery 0.1336 0.0382 0.0274 0.0324 0.015 0.0553 0.0886 0.0209 0.0302 0.0264 0.0537 0.0638 0.1418 0.0243 0.0159 0.0184 0.0292 0.0574
Nucleolus 0.0011 0.0003 0.0002 0 0.0003 0 0.0007 0.0001 0.0001 0.0001 0.0001 0.0012 0.0017 0.0001 0.0002 0.0001 0.0008 0.0003
Nucleus 0.0118 0.0016 0.001 0.0009 0.0006 0.0007 0.006 0.0009 0.0028 0.0007 0.0018 0.0073 0.0057 0.0007 0.0006 0.0011 0.0022 0.0023
Peroxisomes 0.0162 0.0006 0.0008 0 0.0048 0.0002 0.0008 0.0001 0.0002 0.0005 0.0001 0 0.0009 0.0002 0.0002 0.0001 0.0008 0.0001
Punctate Nuclear 0.0105 0.0003 0.0004 0 0.003 0.0001 0.0063 0.0002 0.001 0.0001 0.0001 0.0005 0.0054 0.0002 0.0001 0.0002 0.0005 0.0004
Vacuole 0.0174 0.0143 0.0065 0.0039 0.0196 0.0031 0.0085 0.0078 0.0042 0.006 0.0035 0.003 0.0095 0.0044 0.0038 0.004 0.017 0.0032
Vacuole Periphery 0.0191 0.007 0.0063 0.0038 0.0244 0.0045 0.0151 0.0043 0.0052 0.0096 0.0069 0.0064 0.0104 0.0029 0.0029 0.0036 0.0267 0.0051

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 274.4845 236.6465 186.9979 217.3098 227.8045 314.5264 221.0982 193.6489 205.2746 234.0687
Translational Efficiency 3.0816 3.1277 3.1154 2.484 3.107 2.4995 2.2072 2.5351 2.352 2.3611

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1043 735 175 16 1133 28 100 20 2176 763 275 36

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 2054.37 2227.59 3426.06 332.77 2575.52 2464.41 5053.71 2842.21 2325.72 2236.28 4017.93 1726.90
Standard Deviation 534.27 594.76 1013.92 3.41 529.00 611.63 673.74 652.48 591.87 597.05 1196.79 1338.44
Intensity Change Log 2 0.116788 0.737854 -2.626099 -0.063621 0.972479 0.142149 0.019225 0.873047 -0.544230

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.011557 0.014035 0.007920 0.000133 0.006498 0.001829 0.000890 0.006630 0.008923 0.013587 0.005364 0.003743
Bud Neck 0.001830 0.001526 0.004742 0.000045 0.000922 0.002723 0.016650 0.004302 0.001357 0.001570 0.009072 0.002410
Bud Site 0.022551 0.005527 0.022905 0.000068 0.001976 0.010687 0.001644 0.005554 0.011838 0.005716 0.015174 0.003116
Cell Periphery 0.013869 0.012251 0.017192 0.002569 0.005987 0.005864 0.022233 0.050146 0.009765 0.012016 0.019025 0.029001
Cytoplasm 0.016388 0.001381 0.003594 0.000902 0.000531 0.007055 0.000297 0.000090 0.008132 0.001589 0.002395 0.000451
Cytoplasmic Foci 0.069344 0.004972 0.013941 0.000032 0.001047 0.013475 0.000487 0.001748 0.033783 0.005284 0.009049 0.000985
Eisosomes 0.001698 0.005334 0.000695 0.005121 0.003759 0.000271 0.003282 0.002405 0.002771 0.005148 0.001636 0.003612
Endoplasmic Reticulum 0.623983* 0.565660* 0.238843* 0.000007 0.879172* 0.650950* 0.455326* 0.520251* 0.756855* 0.568789* 0.317564* 0.289031*
Endosome 0.030490 0.017877 0.037691 0.000002 0.006366 0.046281 0.016491 0.032555 0.017929 0.018920 0.029982 0.018087
Golgi 0.128334* 0.230952* 0.259072* 0.000020 0.059146 0.094473 0.062095 0.183350* 0.092309 0.225944* 0.187444* 0.101870
Lipid Particles 0.040981 0.083496 0.103006* 0.000319 0.014602 0.109228* 0.129948* 0.151961* 0.027246 0.084440 0.112803* 0.084565
Mitochondria 0.002836 0.006239 0.003895 0.000065 0.002345 0.003280 0.000890 0.000065 0.002580 0.006131 0.002802 0.000065
Mitotic Spindle 0.002271 0.000203 0.004265 0.000477 0.000169 0.001131 0.000525 0.000309 0.001176 0.000237 0.002905 0.000384
None 0.000583 0.002167 0.000623 0.987003* 0.000729 0.000341 0.000134 0.000101 0.000659 0.002100 0.000445 0.438724*
Nuclear Periphery 0.000770 0.001357 0.004822 0.000002 0.001997 0.000964 0.057960 0.001134 0.001408 0.001343 0.024145 0.000631
Nuclear Periphery Foci 0.006306 0.002328 0.022761 0.000004 0.001893 0.004679 0.059996 0.002801 0.004008 0.002414 0.036301 0.001558
Nucleolus 0.000691 0.001949 0.000071 0.000007 0.000024 0.000028 0.000073 0.000004 0.000343 0.001878 0.000072 0.000005
Nucleus 0.000759 0.004606 0.000322 0.000038 0.000279 0.004896 0.000307 0.000080 0.000509 0.004616 0.000317 0.000061
Peroxisomes 0.000507 0.000285 0.003407 0.003185 0.000074 0.000089 0.000006 0.000086 0.000281 0.000278 0.002170 0.001464
Vacuole 0.010882 0.014363 0.172659 0.000002 0.004190 0.032221 0.137978 0.029665 0.007398 0.015018 0.160048 0.016481
Vacuole Periphery 0.013371 0.023493 0.077573 0.000000 0.008295 0.009536 0.032786 0.006762 0.010728 0.022981 0.061287 0.003757

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.77 0.73 6.14 5.30 1.68 2.30 3.62 -0.02 -0.94 -1.25 -1.66 1.13 1.83 2.73 0.41
Bud Neck 0.77 -2.21 5.06 6.04 3.13 -1.31 -6.16 -1.31 -0.37 3.47 -0.72 -5.39 -0.70 -0.54 3.61
Bud Site 6.80 -0.33 11.20 3.68 3.67 -1.47 0.50 -1.78 0.92 -2.02 3.39 -1.02 5.71 1.46 2.96
Cell Periphery 0.68 -1.02 4.72 3.65 4.40 -0.05 -5.47 -1.84 -1.84 -1.15 -1.10 -4.17 -1.39 -1.22 -0.71
Cytoplasm 6.21 5.17 6.45 0.50 2.41 -1.62 1.08 1.61 1.81 1.88 4.92 4.58 6.64 1.47 3.02
Cytoplasmic Foci 14.04 10.31 15.39 6.06 5.02 -1.60 2.38 -1.22 1.50 -2.27 11.69 8.30 14.23 4.80 4.41
Eisosomes -2.77 1.55 -0.78 0.05 -1.02 4.87 0.30 1.00 -1.85 0.37 -1.94 1.45 -0.41 0.66 -0.96
Endoplasmic Reticulum 3.66 14.62 57.07* 49.88* 9.67 3.66 12.33 4.44 1.19 -0.83 14.49 20.37 7.47 4.42 0.35
Endosome 7.09 -1.57 22.00 15.95 8.68 -4.39 -6.75 -2.49 1.01 -1.49 -0.72 -4.07 -0.02 0.13 1.72
Golgi -10.22 -5.80 21.99 28.23 12.15 -1.65 -0.24 -2.27 -1.51 -2.21 -15.48 -6.08 -0.29 3.56 2.22
Lipid Particles -7.27 -4.59 13.64 16.53 7.82 -3.01 -5.79 -2.80 -0.77 -0.41 -10.95 -7.65 -1.92 -0.01 0.88
Mitochondria -1.87 -0.58 4.65 3.60 2.35 -0.52 1.70 2.76 1.03 3.31 -2.06 -0.15 4.87 3.69 2.62
Mitotic Spindle 3.70 -1.51 3.00 -0.92 2.84 -0.95 -1.69 -1.01 0.80 0.98 3.30 -2.03 2.71 -0.77 2.95
None -2.53 -0.16 -93.52* -93.21* -93.52* 0.94 2.62 2.75 1.14 0.78 -2.30 1.68 -5.28 -5.26 -5.28
Nuclear Periphery -3.73 -4.61 9.06 10.29 5.49 1.19 -6.94 1.79 0.06 7.05 0.29 -6.78 2.78 2.81 7.00
Nuclear Periphery Foci 4.41 -4.26 7.68 6.13 5.92 -2.07 -5.37 -1.33 1.41 5.29 2.72 -6.82 4.26 1.70 7.33
Nucleolus -1.15 0.98 1.10 2.15 5.08 -0.43 -2.66 2.76 1.08 4.10 -1.67 0.89 1.14 2.16 6.60
Nucleus -2.02 1.07 2.15 2.47 2.96 -0.93 0.62 1.14 1.00 4.55 -2.20 1.08 2.17 2.58 3.89
Peroxisomes 1.52 -0.92 -0.94 -1.01 0.04 -0.27 2.00 -0.25 0.03 -2.22 0.03 -0.94 -0.93 -0.93 0.29
Vacuole -1.45 -9.17 7.97 7.43 9.81 -2.38 -8.97 -2.95 0.31 6.38 -3.74 -12.24 -1.67 -0.23 10.66
Vacuole Periphery -4.06 -7.06 10.16 11.13 8.64 -0.22 -3.93 0.68 0.46 3.99 -5.58 -8.00 4.62 7.94 9.00
Standard name
Human Ortholog
Description Component of mRNP complexes associated with polyribosomes; involved in localization of mRNAs to P bodies; implicated in secretion and nuclear segregation; multicopy suppressor of BFA (Brefeldin A) sensitivity
Localization
Cell Percentages ER (83%)
Cell Cycle Regulation No
Subcompartmental Group ER-5

Bfr1

Bfr1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Bfr1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available