Standard name
Human Ortholog
Description Protein required for mitochondrial ribosome small subunit biogenesis; null mutant is defective in respiration and in maturation of 15S rRNA; protein is localized to the mitochondrial inner membrane; null mutant interacts synthetically with prohibitin (Phb1p)
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.08 0 0.09 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0.05 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0.06 0 0 0 0 0 – 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0
Cytoplasm 0.24 0.44 0.63 0.27 0.3 0.18 0.16 – 0.15 0.07 0.11 0.11 0.67 0.76 0.86 0.29 0.23 0.23 0 0.08 0 0.07 0.07 0.11
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.09 0.06 0.07 0 0 0 0 0 0
Endosome 0 0.1 0 0 0.11 0.05 0.09 – 0 0 0 0 0.09 0.08 0 0.15 0.24 0.29 0.09 0.09 0.12 0.06 0 0
Golgi 0 0 0.05 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.73 0.25 0.05 0.39 0.28 0.6 0.61 – 0.79 0.77 0.9 0.8 0.08 0 0 0.15 0.14 0.14 0.66 0.7 0.63 0.66 0.66 0.6
Nucleus 0 0 0 0 0 0 0.05 – 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0.08 0.05 0 0.17 0.14 0 0 0 0 0 0
SpindlePole 0.05 0 0.11 0.17 0.18 0.11 0.18 – 0.09 0.18 0.05 0.17 0.05 0 0 0.25 0.17 0.16 0 0 0 0 0 0
Vac/Vac Membrane 0 0.13 0.21 0.07 0.16 0.08 0 – 0 0 0 0.06 0.06 0.05 0 0.14 0.1 0.15 0 0 0.12 0.05 0.08 0.09
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 4 5 0 13 2 3 1 – 0 3 0 0 7 4 0 3 4 5 5 1 1 2 6 11
Bud 6 0 0 4 6 3 12 – 13 18 11 11 6 1 0 10 8 6 1 0 0 0 4 4
Bud Neck 1 0 0 1 4 1 1 – 3 6 3 1 1 0 3 0 4 4 2 3 0 0 2 2
Bud Site 2 0 0 1 1 1 2 – 3 8 4 8 0 0 0 0 1 4 – – – – – –
Cell Periphery 4 4 0 4 1 1 0 – 1 4 1 6 2 4 4 11 11 10 0 0 0 0 0 1
Cytoplasm 42 28 12 38 54 43 52 – 43 26 36 44 109 227 375 48 66 72 4 8 3 4 19 29
Endoplasmic Reticulum 7 0 0 4 1 6 3 – 0 5 6 1 1 6 2 15 18 22 4 0 1 1 2 1
Endosome 0 6 0 4 21 13 31 – 3 7 1 5 15 25 19 25 70 88 21 9 10 4 11 11
Golgi 0 0 1 0 0 0 1 – 0 0 0 0 0 0 1 1 3 3 0 0 1 0 0 0
Mitochondria 129 16 1 56 51 146 203 – 230 276 306 323 13 7 6 25 41 43 148 72 53 45 179 155
Nucleus 0 0 0 2 5 8 18 – 14 15 6 7 1 3 2 3 7 3 0 0 0 0 1 1
Nuclear Periphery 0 0 0 1 0 3 1 – 0 0 0 1 0 0 0 0 0 0 3 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 3 – 1 1 0 1 0 0 0 0 1 1 0 0 0 0 1 0
Peroxisomes 1 2 0 2 4 3 4 – 0 2 2 7 1 25 23 5 48 43 9 1 0 1 1 1
SpindlePole 9 2 2 24 33 28 58 – 26 64 17 67 8 10 17 41 49 50 8 1 0 0 7 5
Vac/Vac Membrane 4 8 4 10 29 20 13 – 9 15 8 24 9 15 11 23 28 47 9 2 10 3 21 22
Unique Cell Count 177 63 19 142 183 245 331 292 360 340 403 163 297 435 166 287 307 224 104 85 70 270 257
Labelled Cell Count 209 71 20 164 212 279 403 346 450 401 506 173 327 463 210 359 401 224 104 85 70 270 257


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.6 4.8 5.8 5.1 5.5 4.5 4.6 – 4.0 4.0 3.5 4.0 5.6 5.4 5.5 5.8 5.8 5.6 – 5.3 5.5
Std Deviation (1e-4) 0.4 1.3 1.0 1.1 1.2 1.1 1.2 – 1.3 0.8 1.1 1.0 1.5 1.2 1.7 1.1 1.4 1.3 – 1.3 1.7
Intensity Change (Log2) – – – -0.19 -0.08 -0.36 -0.33 – -0.54 -0.52 -0.73 -0.55 -0.04 -0.09 -0.08 0.01 -0.0 -0.06 – -0.14 -0.06


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.243 0.5963 0.4673 -0.32 0.431 0.4961 0.2077 0.4887 0.1931 -0.1534 0.6376 0.2145 -0.8287 -0.8071 -0.8515 -1.0519 -1.0849 -0.9423
Actin 0.008 0.0057 0.003 0.0012 0.0067 0.0068 0.0429 0.0302 0.026 0.0561 0.0015 0.0275 0.039 0.0335 0.0287 0.0598 0.0028 0.0342
Bud 0.0014 0.0024 0.0019 0.0031 0.0084 0.0007 0.0013 0.0029 0.0019 0.0027 0.0006 0.0016 0.0014 0.0035 0.0038 0.0015 0.0027 0.0008
Bud Neck 0.0011 0.001 0.002 0.0008 0.003 0.0035 0.0092 0.002 0.0032 0.0121 0.0006 0.0063 0.0033 0.0019 0.0035 0.0007 0.0007 0.009
Bud Periphery 0.0036 0.0077 0.0053 0.0198 0.0159 0.0023 0.0029 0.0052 0.0039 0.0077 0.0016 0.004 0.0023 0.0047 0.0073 0.0039 0.007 0.0018
Bud Site 0.0062 0.0166 0.0128 0.0016 0.0242 0.0011 0.0177 0.0496 0.0189 0.0053 0.0007 0.004 0.0139 0.0448 0.033 0.0064 0.0012 0.0016
Cell Periphery 0.0016 0.0012 0.001 0.001 0.0016 0.0014 0.0104 0.0017 0.0035 0.0007 0.0003 0.0017 0.0016 0.0031 0.0072 0.0006 0.0004 0.0006
Cytoplasm 0.0114 0.0215 0.0217 0.0077 0.0271 0.0142 0.0188 0.0352 0.0623 0.0497 0.0047 0.0538 0.0426 0.0447 0.0558 0.0162 0.0164 0.0245
Cytoplasmic Foci 0.0596 0.072 0.0776 0.0464 0.071 0.0663 0.1381 0.1305 0.077 0.0801 0.047 0.0822 0.1178 0.1113 0.0964 0.0606 0.109 0.0787
Eisosomes 0.0027 0.0017 0.0005 0.0002 0.0003 0.0009 0.0031 0.0007 0.0026 0.0008 0.0001 0.0016 0.0008 0.0019 0.0013 0.001 0.0001 0.0005
Endoplasmic Reticulum 0.0036 0.002 0.0024 0.0014 0.0033 0.001 0.0097 0.004 0.0073 0.0034 0.0013 0.0028 0.0102 0.0042 0.0035 0.0019 0.0009 0.001
Endosome 0.1324 0.1506 0.0966 0.0978 0.0637 0.0817 0.1367 0.1303 0.1198 0.1012 0.1459 0.1054 0.194 0.1428 0.1268 0.2036 0.1493 0.0748
Golgi 0.0562 0.0514 0.0451 0.0406 0.0438 0.045 0.0746 0.0881 0.0609 0.0305 0.0564 0.072 0.0788 0.0738 0.0783 0.0457 0.0776 0.0729
Lipid Particles 0.0687 0.0241 0.0238 0.0474 0.0151 0.039 0.0731 0.0423 0.0128 0.0089 0.0068 0.0427 0.0794 0.0329 0.0256 0.0054 0.0095 0.0274
Mitochondria 0.4779 0.5321 0.5469 0.6411 0.5928 0.5802 0.2874 0.3386 0.4856 0.5802 0.6691 0.5056 0.2681 0.3629 0.4131 0.555 0.5303 0.4958
None 0.0783 0.0366 0.0541 0.0213 0.0254 0.0633 0.0388 0.0513 0.0573 0.0145 0.0012 0.0272 0.0337 0.0601 0.0644 0.0019 0.0063 0.0742
Nuclear Periphery 0.0024 0.0011 0.0035 0.0007 0.0089 0.0009 0.0034 0.001 0.0013 0.0008 0.0002 0.0004 0.0047 0.0011 0.0008 0.0005 0.0004 0.0005
Nucleolus 0.0041 0.0015 0.0023 0.0009 0.0013 0.0023 0.0059 0.0015 0.001 0.0005 0.0001 0.0007 0.005 0.0009 0.0013 0.0002 0.0002 0.0013
Nucleus 0.002 0.0017 0.0025 0.0019 0.0126 0.0021 0.0031 0.0021 0.0014 0.0013 0.0002 0.001 0.0042 0.001 0.0012 0.0005 0.0004 0.0009
Peroxisomes 0.0654 0.0485 0.0794 0.0467 0.0642 0.0727 0.0968 0.0656 0.0362 0.0307 0.0519 0.0245 0.0662 0.0555 0.0305 0.0228 0.0741 0.0909
Punctate Nuclear 0.0019 0.0008 0.001 0.0004 0.0009 0.0065 0.0136 0.0008 0.0007 0.0013 0.0002 0.0004 0.0093 0.0011 0.0015 0.0003 0.0003 0.0014
Vacuole 0.0074 0.0129 0.012 0.0113 0.0067 0.0049 0.009 0.0121 0.0126 0.0078 0.0043 0.027 0.0174 0.0105 0.0112 0.0067 0.0061 0.0044
Vacuole Periphery 0.0041 0.0067 0.0047 0.0067 0.0032 0.0033 0.0033 0.004 0.0039 0.004 0.0052 0.0076 0.0062 0.0039 0.0048 0.0048 0.0044 0.003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 15.1875 7.0835 11.8266 14.2082 8.1333 13.1992 15.0703 12.6891 10.6188 15.868
Translational Efficiency 0.3381 0.6892 0.3908 0.3153 0.4725 0.4429 0.3403 0.3766 0.5093 0.3005

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1339 340 343 705 161 1532 151 2640 1500 1872 494 3345

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 624.97 728.50 931.03 862.66 755.83 719.39 949.86 864.99 639.02 721.04 936.79 864.50
Standard Deviation 70.59 104.95 119.45 121.53 95.86 84.84 91.03 110.10 84.11 88.90 111.87 112.61
Intensity Change Log 2 — 0.221142 0.575041 0.465005 — -0.071288 0.329653 0.194622 — 0.068448 0.445911 0.323307

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000287 0.004415 0.012123 0.011884 0.000681 0.000897 0.003547 0.016676 0.000329 0.001536 0.009501 0.015666
Bud Neck 0.042428 0.028576 0.020770 0.019067 0.050572 0.018247 0.020408 0.022487 0.043303 0.020123 0.020659 0.021766
Bud Site 0.014525 0.039338 0.066396 0.072348 0.021024 0.016380 0.018933 0.101009* 0.015223 0.020550 0.051888 0.094969
Cell Periphery 0.000236 0.000384 0.000385 0.000246 0.000475 0.000230 0.000282 0.000203 0.000262 0.000258 0.000353 0.000212
Cytoplasm 0.139275* 0.031301 0.120177* 0.043776 0.046743 0.021955 0.083324 0.092892 0.129343* 0.023652 0.108912* 0.082540
Cytoplasmic Foci 0.319838* 0.183933* 0.135771 0.153192 0.238452* 0.166588 0.247135* 0.114636 0.311102* 0.169739 0.169812 0.122762
Eisosomes 0.000221 0.000232 0.000180 0.000157 0.000264 0.000174 0.000245 0.000170 0.000225 0.000184 0.000200 0.000167
Endoplasmic Reticulum 0.001552 0.001082 0.027935 0.003137 0.002065 0.000546 0.007099 0.001313 0.001607 0.000643 0.021566 0.001697
Endosome 0.073282 0.114731 0.045912 0.105030 0.143256 0.110248 0.101116 0.050071 0.080793 0.111062 0.062786 0.061654
Golgi 0.038058 0.099511 0.015525 0.078144 0.098276 0.126833* 0.018947 0.070499 0.044522 0.121871* 0.016571 0.072111
Lipid Particles 0.030047 0.008526 0.016476 0.005167 0.036243 0.008328 0.024903 0.001742 0.030712 0.008364 0.019052 0.002464
Mitochondria 0.173022* 0.229917* 0.015244 0.147621* 0.205928* 0.390213* 0.018839 0.223361* 0.176554* 0.361100* 0.016343 0.207397*
Mitotic Spindle 0.003727 0.024456 0.127134* 0.111177* 0.017490 0.018128 0.047070 0.097514 0.005204 0.019278 0.102661* 0.100394*
None 0.003262 0.001447 0.001075 0.000336 0.000730 0.000506 0.002210 0.000350 0.002990 0.000677 0.001422 0.000347
Nuclear Periphery 0.000593 0.000130 0.003233 0.001083 0.001394 0.000193 0.002349 0.000374 0.000679 0.000182 0.002963 0.000524
Nuclear Periphery Foci 0.000349 0.000269 0.010031 0.002371 0.001908 0.000208 0.004372 0.000417 0.000517 0.000219 0.008301 0.000829
Nucleolus 0.003225 0.001066 0.004827 0.001550 0.004181 0.001217 0.006764 0.000464 0.003328 0.001189 0.005419 0.000693
Nucleus 0.036339 0.005672 0.120376 0.007351 0.010640 0.005239 0.086278 0.007465 0.033581 0.005317 0.109953 0.007441
Peroxisomes 0.040826 0.187922* 0.066112 0.101529* 0.069127 0.066519 0.095887 0.105716* 0.043864 0.088569 0.075214 0.104833*
Vacuole 0.067911 0.030162 0.182129 0.119006 0.029379 0.032059 0.193882 0.080794 0.063775 0.031715 0.185722 0.088848
Vacuole Periphery 0.010996 0.006929 0.008190 0.015828 0.021171 0.015292 0.016407 0.011846 0.012088 0.013773 0.010702 0.012685

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.97 -8.28 -6.07 -3.62 0.15 -0.85 -4.84 -13.02 -12.78 -9.46 -5.78 -9.01 -14.71 -13.26 -4.10
Bud Neck 2.83 6.07 7.12 1.88 1.37 3.10 2.88 2.61 -2.82 -1.20 7.84 7.03 7.48 -1.51 -0.35
Bud Site -5.05 -9.72 -12.04 -5.18 -1.43 1.03 0.34 -15.15 -25.19* -21.51* -2.93 -9.25 -27.92 -24.96 -9.78
Cell Periphery -2.34 -5.17 -0.11 2.07 3.87 1.67 1.24 1.91 1.33 3.14 0.12 -3.71 2.42 2.15 6.57
Cytoplasm 14.10 1.96 13.32 -2.60 7.69 2.91 -2.92 -5.82 -22.33 -1.39 20.25 2.50 7.46 -21.15 3.05
Cytoplasmic Foci 12.07 20.97 19.43 3.49 -3.27 4.30 -0.16 7.65 10.72 8.10 19.87 16.31 29.68 10.89 4.58
Eisosomes -0.50 1.85 4.27 3.32 2.40 2.11 0.27 2.40 0.32 4.39 2.26 1.04 4.65 1.16 3.82
Endoplasmic Reticulum 0.93 -10.27 -3.65 -3.48 9.56 2.38 -5.08 1.01 -5.61 7.06 4.17 -10.79 -0.87 -6.65 10.71
Endosome -4.58 7.09 -4.58 1.29 -9.93 2.15 2.74 6.37 14.96 4.76 -6.37 4.43 5.49 13.33 -0.30
Golgi -7.63 7.70 -6.48 2.74 -10.84 -2.16 6.12 2.40 12.14 -10.61 -17.29 9.51 -7.43 12.20 -17.86
Lipid Particles 8.46 5.56 12.44 1.69 6.00 3.41 1.39 4.22 7.01 6.49 11.09 4.98 15.12 6.93 10.23
Mitochondria -3.31 22.21 2.93 4.99 -13.72 -8.34 8.96 -0.44 17.67 -28.34* -18.51 23.75 -2.86 18.28 -36.62*
Mitotic Spindle -4.43 -10.54 -12.88 -9.14 0.42 -0.06 -2.52 -11.68 -19.08 -4.91 -7.10 -11.00 -26.90 -21.16 -0.68
None 2.12 4.45 5.98 1.43 4.24 1.00 -1.23 2.01 1.35 1.49 5.07 2.31 6.17 1.95 2.50
Nuclear Periphery 3.75 -7.32 -1.87 -3.93 5.49 1.19 -0.82 0.97 -3.21 4.54 3.22 -7.50 0.72 -4.98 8.51
Nuclear Periphery Foci 0.98 -9.11 -2.70 -2.81 6.31 1.68 -2.41 1.45 -2.38 8.17 2.37 -10.07 -1.57 -3.55 9.59
Nucleolus 3.99 -2.26 2.78 -1.05 3.98 2.14 -1.23 2.68 2.83 3.97 5.13 -2.88 6.82 1.82 6.72
Nucleus 10.46 -9.38 9.51 -1.50 12.34 2.22 -6.20 0.48 -3.34 6.38 11.57 -10.40 10.10 -3.64 13.83
Peroxisomes -10.74 -2.29 -7.96 5.91 -4.17 0.28 -1.18 -3.60 -7.52 -1.14 -10.03 -3.43 -15.08 -2.79 -5.03
Vacuole 6.20 -12.43 -8.00 -12.34 6.02 -0.58 -9.91 -11.12 -16.03 6.72 7.52 -14.83 -7.76 -20.04 11.56
Vacuole Periphery 3.69 3.37 -2.70 -5.09 -4.87 1.22 1.11 2.05 3.27 0.79 -1.55 1.82 -0.11 1.58 -1.98
Standard name
Human Ortholog
Description Protein required for mitochondrial ribosome small subunit biogenesis; null mutant is defective in respiration and in maturation of 15S rRNA; protein is localized to the mitochondrial inner membrane; null mutant interacts synthetically with prohibitin (Phb1p)
Localization
Cell Percentages mitochondrion (54%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Gep3

Gep3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gep3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available