Standard name | |
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Human Ortholog | |
Description | Subunit of the SAS complex (Sas2p, Sas4p, Sas5p); acetylates free histones and nucleosomes and regulates transcriptional silencing; stimulates Sas2p HAT activity |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 4 | 3 | 6 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 1 | 5 |
Bud Neck | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 5 | 1 | 7 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 1 | 0 | 2 | 4 | 5 | 9 | 9 | 9 | 12 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1 | 4 | 2 | 5 | 15 | 19 | 23 | 45 | 18 | 19 | 12 | 16 | 8 | 22 | 40 | 15 | 20 | 5 | 0 | 1 | 0 | 1 | 6 | 2 |
Endoplasmic Reticulum | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 6 | 2 | 0 | 0 | 0 | 0 | 0 | 1 |
Endosome | 0 | 0 | 1 | 1 | 2 | 2 | 1 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 2 | 2 | 0 | 0 | 2 | 2 | 1 | 3 | 3 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 |
Mitochondria | 11 | 112 | 2 | 10 | 3 | 40 | 53 | 56 | 167 | 129 | 115 | 125 | 4 | 4 | 3 | 8 | 14 | 10 | 0 | 5 | 0 | 0 | 2 | 5 |
Nucleus | 150 | 172 | 162 | 93 | 140 | 161 | 175 | 191 | 153 | 182 | 130 | 133 | 84 | 142 | 147 | 44 | 100 | 69 | 140 | 177 | 148 | 103 | 123 | 110 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Nucleolus | 6 | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 1 | 3 | 2 | 3 | 2 | 3 | 0 | 1 | 6 | 4 | 7 | 7 | 12 | 7 | 20 | 25 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 4 | 10 | 3 | 12 | 5 | 10 | 18 | 9 | 15 | 5 | 6 | 3 | 5 | 4 | 1 | 2 | 3 | 1 | 3 | 4 | 2 | 5 | 9 |
Vac/Vac Membrane | 1 | 2 | 1 | 0 | 3 | 2 | 5 | 1 | 1 | 1 | 0 | 8 | 1 | 1 | 4 | 5 | 3 | 1 | 8 | 22 | 8 | 7 | 15 | 25 |
Unique Cell Count | 158 | 214 | 167 | 100 | 163 | 203 | 224 | 282 | 248 | 269 | 189 | 216 | 95 | 168 | 195 | 66 | 129 | 81 | 162 | 226 | 181 | 133 | 184 | 199 |
Labelled Cell Count | 169 | 295 | 178 | 115 | 176 | 234 | 274 | 325 | 366 | 370 | 282 | 309 | 102 | 177 | 201 | 78 | 153 | 94 | 162 | 226 | 181 | 133 | 184 | 199 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.4 | 3.8 | 5.8 | 5.3 | 5.8 | 4.7 | 4.6 | 4.8 | 3.6 | 4.0 | 4.2 | 3.9 | 5.4 | 5.5 | 5.3 | 5.8 | 6.2 | 6.6 | 6.4 | 6.5 | 6.5 |
Std Deviation (1e-4) | 0.7 | 0.6 | 0.8 | 1.4 | 1.5 | 1.4 | 1.5 | 1.3 | 0.7 | 1.0 | 2.9 | 1.6 | 1.1 | 0.9 | 1.4 | 1.8 | 1.7 | 1.4 | 1.4 | 1.6 | 1.8 |
Intensity Change (Log2) | – | – | – | -0.13 | 0.02 | -0.28 | -0.32 | -0.27 | -0.68 | -0.54 | -0.47 | -0.55 | -0.09 | -0.07 | -0.11 | 0.02 | 0.11 | 0.19 | 0.15 | 0.17 | 0.17 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.4232 | 0.5806 | 0.2819 | -0.0009 | 0.1562 | 0.4766 | 3.7471 | 3.6707 | 4.4525 | 4.3427 | 4.3701 | 3.9186 | 2.0527 | 2.6233 | 2.37 | 2.098 | 2.2174 | 2.1283 |
Actin | 0.0017 | 0.0003 | 0.0005 | 0 | 0.0125 | 0.0014 | 0.0033 | 0 | 0.0024 | 0.0001 | 0.0001 | 0 | 0.0014 | 0.0003 | 0.004 | 0.0022 | 0.0003 | 0 |
Bud | 0.0005 | 0.0003 | 0 | 0 | 0.0001 | 0 | 0.0007 | 0 | 0.0012 | 0.0001 | 0 | 0 | 0.0002 | 0.0002 | 0 | 0.0001 | 0.0001 | 0 |
Bud Neck | 0.0014 | 0.0003 | 0.0003 | 0 | 0.0004 | 0.0009 | 0.0225 | 0.0003 | 0.0028 | 0.0001 | 0.0009 | 0.0013 | 0.0004 | 0.0003 | 0.0004 | 0.0001 | 0.0004 | 0.0019 |
Bud Periphery | 0.0004 | 0.0003 | 0.0001 | 0 | 0.0002 | 0 | 0.0019 | 0 | 0.002 | 0.0002 | 0 | 0 | 0.0009 | 0.0001 | 0 | 0.0001 | 0.0002 | 0.0001 |
Bud Site | 0.0004 | 0.0083 | 0.0001 | 0 | 0.0002 | 0 | 0.0063 | 0.0001 | 0.0082 | 0.0002 | 0.0001 | 0.0001 | 0.0005 | 0.0004 | 0.0003 | 0.0004 | 0.0005 | 0.0001 |
Cell Periphery | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0.0009 | 0 | 0.0007 | 0.0001 | 0 | 0 | 0.0008 | 0 | 0 | 0 | 0.0001 | 0 |
Cytoplasm | 0.0074 | 0.0047 | 0.0007 | 0.0002 | 0.0038 | 0.003 | 0.0034 | 0.0003 | 0.0076 | 0.0005 | 0.0065 | 0.0046 | 0.0056 | 0.0043 | 0.0013 | 0.0005 | 0.0008 | 0.0004 |
Cytoplasmic Foci | 0.0041 | 0.0016 | 0.0001 | 0 | 0.0009 | 0.0005 | 0.0023 | 0 | 0.0017 | 0.0009 | 0.0003 | 0.0001 | 0.0065 | 0.0002 | 0.0046 | 0.0017 | 0.0008 | 0 |
Eisosomes | 0.0001 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0.0004 | 0 | 0.0001 | 0 | 0.0003 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0018 | 0.0005 | 0.0011 | 0 | 0.0023 | 0.0001 | 0.0008 | 0 | 0.0007 | 0.0009 | 0.0004 | 0 | 0.0006 | 0.0002 | 0.0001 | 0.0002 | 0.0004 | 0 |
Endosome | 0.0073 | 0.0007 | 0.003 | 0 | 0.0084 | 0.0001 | 0.0027 | 0 | 0.0053 | 0.0436 | 0.0004 | 0 | 0.0023 | 0.0001 | 0.0017 | 0.0043 | 0.0148 | 0 |
Golgi | 0.0013 | 0.0005 | 0.0002 | 0 | 0.0033 | 0.0001 | 0.0008 | 0 | 0.0023 | 0.0067 | 0 | 0 | 0.0006 | 0 | 0.0021 | 0.0022 | 0.0041 | 0 |
Lipid Particles | 0.0047 | 0.0001 | 0.0002 | 0 | 0.0023 | 0.0002 | 0.0017 | 0 | 0.0053 | 0.0007 | 0.0002 | 0 | 0.0047 | 0 | 0.0011 | 0.0095 | 0.0043 | 0 |
Mitochondria | 0.0013 | 0.0006 | 0.0004 | 0.0001 | 0.0011 | 0.0001 | 0.0044 | 0.0001 | 0.0092 | 0.0065 | 0.0002 | 0.0001 | 0.0013 | 0.0002 | 0.0005 | 0.0122 | 0.0058 | 0.0001 |
None | 0.0099 | 0.0014 | 0.0026 | 0 | 0.0028 | 0.003 | 0.032 | 0 | 0.0557 | 0.0001 | 0.1468 | 0.0237 | 0.0013 | 0.0178 | 0.0052 | 0.0006 | 0.0003 | 0.0001 |
Nuclear Periphery | 0.0148 | 0.0183 | 0.0089 | 0.0007 | 0.0207 | 0.0013 | 0.0036 | 0.0008 | 0.0028 | 0.0006 | 0.0035 | 0.0004 | 0.0125 | 0.0033 | 0.0042 | 0.0009 | 0.0042 | 0.0017 |
Nucleolus | 0.0086 | 0.0069 | 0.0065 | 0.0051 | 0.0157 | 0.0189 | 0.0221 | 0.0136 | 0.0104 | 0.006 | 0.0218 | 0.0143 | 0.0109 | 0.0096 | 0.0051 | 0.0034 | 0.0231 | 0.0157 |
Nucleus | 0.9157 | 0.9301 | 0.9709 | 0.9929 | 0.9174 | 0.9626 | 0.88 | 0.9842 | 0.8644 | 0.9211 | 0.8025 | 0.9502 | 0.9096 | 0.9434 | 0.964 | 0.9193 | 0.9244 | 0.9562 |
Peroxisomes | 0.0025 | 0.0001 | 0 | 0 | 0.0002 | 0 | 0.0021 | 0 | 0.0017 | 0 | 0 | 0 | 0.0116 | 0.0001 | 0.0025 | 0.0171 | 0.0004 | 0 |
Punctate Nuclear | 0.0142 | 0.024 | 0.0033 | 0.0008 | 0.0066 | 0.0076 | 0.005 | 0.0005 | 0.0115 | 0.001 | 0.0154 | 0.0049 | 0.0249 | 0.0192 | 0.0018 | 0.0244 | 0.011 | 0.0235 |
Vacuole | 0.0013 | 0.0007 | 0.0007 | 0 | 0.0004 | 0.0001 | 0.0026 | 0.0001 | 0.0034 | 0.0081 | 0.0004 | 0.0001 | 0.0021 | 0.0001 | 0.0003 | 0.0004 | 0.0023 | 0.0001 |
Vacuole Periphery | 0.0007 | 0.0003 | 0.0004 | 0 | 0.0005 | 0 | 0.0006 | 0 | 0.0007 | 0.0025 | 0.0001 | 0 | 0.0015 | 0 | 0.0006 | 0.0003 | 0.0017 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 10.3398 | 5.2818 | 7.8386 | 11.28 | 12.4484 | 10.3341 | 12.016 | 14.8683 | 8.937 | 15.7847 |
Translational Efficiency | 1.0456 | 1.5239 | 0.8483 | 0.8827 | 0.7615 | 1.1297 | 0.9768 | 0.7107 | 1.1557 | 0.7116 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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