Standard name
Human Ortholog
Description Golgi matrix protein; involved in the structural organization of the cis-Golgi; interacts genetically with COG3 and USO1
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.14 0.1 0.09 0.12 0.11 0.08 0 0 0.06 0 0 0 0.06 0.07 0 0.14 0.18 0.26 0.05 0.1 0 0.12 0.11 0.11
Bud 0.05 0 0 0 0 0 0 0.05 0.1 0.05 0.1 0.09 0 0.07 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.11 0.18 0.08 0.1 0.08 0.09 0.06 0.07 0.07 0.06 0.05 0 0 0.07 0.14 0.08 0.06 0 0.05 0 0 0 0
Endoplasmic Reticulum 0 0.06 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.23 0 0.13 0 0.19 0.14 0.2 0.16 0.06 0 0.06 0 0.12 0.14 0.28 0.24 0.15 0.14 0.18 0.19 0.29 0.15 0.14 0.11
Golgi 0.37 0.44 0.27 0.43 0.3 0.1 0.06 0 0 0.05 0 0 0.64 0.63 0.5 0.49 0.65 0.6 0.67 0.55 0.47 0.6 0.65 0.66
Mitochondria 0.67 0.69 0.71 0.69 0.53 0.69 0.69 0.66 0.79 0.82 0.82 0.83 0.47 0.4 0.3 0.08 0.14 0.12 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0.05 0 0 0.05 0 0 0.05 0.05 0 0 0 0 0.06 0 0.07 0.06 0.05 0.05
SpindlePole 0 0 0 0 0 0 0.05 0.1 0.05 0.07 0.06 0.05 0 0 0 0.06 0.14 0.08 0 0 0 0 0 0
Vac/Vac Membrane 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.1 0 0.05 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 59 7 5 6 12 7 5 7 7 0 4 7 10 19 8 21 18 20 22 9 3 21 27 30
Bud 21 1 1 0 4 3 7 11 11 7 12 19 5 17 8 4 2 1 2 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 1 0 4 0 3 3 2 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 1 0 0 0 0 1 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0
Cytoplasm 8 8 10 4 11 7 16 13 8 10 8 12 7 11 21 22 8 5 1 4 0 1 2 3
Endoplasmic Reticulum 12 4 2 1 4 4 5 9 7 1 0 4 6 1 6 3 2 1 0 1 1 1 1 5
Endosome 95 2 7 2 20 13 36 32 6 5 7 7 19 36 82 37 15 11 78 16 21 25 35 31
Golgi 157 31 15 21 32 9 10 8 3 7 4 6 103 165 145 75 65 46 298 49 35 104 168 186
Mitochondria 281 48 39 34 57 64 124 134 86 111 103 184 75 103 87 12 14 9 6 1 3 2 3 3
Nucleus 1 0 2 0 0 0 0 0 2 0 1 2 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 1 0 0 0 0 0 3 0 1 1 4 0 1 0 1 0 0 0 0 0 0 0 0 0
Nucleolus 12 0 0 1 1 3 8 7 5 2 4 3 4 2 12 2 1 0 0 0 0 0 0 0
Peroxisomes 7 0 0 2 0 0 9 9 3 7 2 7 8 14 10 2 0 1 27 1 5 10 11 14
SpindlePole 11 0 0 0 4 3 9 20 5 10 8 10 3 1 8 9 14 6 5 0 0 1 1 0
Vac/Vac Membrane 3 4 0 0 2 0 4 3 0 2 0 1 2 5 3 7 1 8 1 4 0 0 1 0
Unique Cell Count 420 70 55 49 108 93 181 204 109 135 125 221 161 260 292 154 100 77 447 90 75 175 258 280
Labelled Cell Count 668 105 81 72 147 114 236 257 145 166 161 264 243 374 391 194 141 108 447 90 75 175 258 280


Golgi, Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.2 9.8 7.7 8.1 7.7 6.9 5.9 6.1 5.8 5.5 5.6 5.4 10.4 10.5 9.9 11.1 12.7 13.7 9.9 10.1 10.7
Std Deviation (1e-4) 1.2 1.5 1.4 1.2 1.2 1.1 1.1 1.2 1.0 1.0 1.4 1.1 1.7 1.6 1.4 2.0 2.4 2.6 1.4 1.7 1.7
Intensity Change (Log2) – – – 0.07 0.0 -0.15 -0.39 -0.33 -0.4 -0.49 -0.46 -0.51 0.44 0.45 0.36 0.53 0.72 0.83 0.37 0.39 0.47


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0.5 0.4 0 0 0 0 0 0 0 0 0 0 0.9 1.5 2.4
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.5 -1.4 -2.0 -1.9 0 -2.1 -2.2 -2.4 0 0 0 0 -0.7 -1.9 -2.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0.9 0.2 1.2 0.5 0 0 0 0 -0.2 0.2 2.4 1.8 0.4 0.3
Golgi 1.7 0.3 -2.8 -4.6 0 -4.7 -4.3 -4.8 0 4.7 4.9 3.1 2.8 4.5 3.7
Mitochondria -0.2 -2.2 -0.3 -0.3 -0.7 1.1 1.7 1.7 2.1 -3.1 -4.2 -5.8 -9.4 -7.1 -7.0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0343 0.0161 0.0585 0.0174 0.0461 0.023 0.0184 0.1583 0.0415 0.0804 0.0025 0.0435 – – – – – –
Bud 0.0004 0.0002 0.0002 0.0002 0.0007 0.0002 0.0004 0.0008 0.0002 0.0002 0.0001 0.0004 – – – – – –
Bud Neck 0.001 0.0004 0.0006 0.0004 0.0011 0.0008 0.0005 0.0009 0.0004 0.0012 0.0002 0.0006 – – – – – –
Bud Periphery 0.0004 0.0001 0.0002 0.0004 0.0005 0.0002 0.0005 0.0005 0.0001 0.0002 0.0001 0.0006 – – – – – –
Bud Site 0.0017 0.0006 0.0016 0.0004 0.0011 0.0004 0.001 0.0585 0.0017 0.0005 0.0002 0.0004 – – – – – –
Cell Periphery 0.0001 0 0 0.0001 0.0001 0.0001 0.0002 0.0005 0.0001 0 0 0.0001 – – – – – –
Cytoplasm 0.0094 0.0075 0.0022 0.0013 0.0054 0.0027 0.0114 0.008 0.0246 0.0013 0.0125 0.0329 – – – – – –
Cytoplasmic Foci 0.1903 0.2747 0.1844 0.1476 0.1486 0.1546 0.1362 0.1898 0.2203 0.2711 0.1943 0.2334 – – – – – –
Eisosomes 0.0001 0 0.0001 0.0003 0.0001 0 0.0001 0.0003 0.0001 0.0001 0 0.0002 – – – – – –
Endoplasmic Reticulum 0.004 0.0005 0.0004 0.0024 0.0008 0.0006 0.0076 0.0008 0.0022 0.0001 0.0044 0.0026 – – – – – –
Endosome 0.2246 0.2184 0.1503 0.1811 0.1878 0.1593 0.2705 0.1795 0.2205 0.1344 0.3082 0.1296 – – – – – –
Golgi 0.4223 0.4152 0.5201 0.5291 0.3696 0.5804 0.3209 0.3665 0.3984 0.4133 0.3105 0.4177 – – – – – –
Lipid Particles 0.0249 0.0089 0.013 0.0234 0.0541 0.0152 0.0122 0.0043 0.0271 0.0131 0.0173 0.0074 – – – – – –
Mitochondria 0.0089 0.0029 0.0124 0.0308 0.0574 0.0147 0.0152 0.0022 0.0021 0.0028 0.0038 0.0095 – – – – – –
None 0.0022 0.0001 0.0002 0.0008 0.0005 0.0001 0.0805 0.0002 0.0056 0.0001 0.0136 0.0019 – – – – – –
Nuclear Periphery 0.0081 0.0001 0.0001 0.0031 0.0238 0.0001 0.0562 0.0001 0.0012 0 0.0038 0.0008 – – – – – –
Nucleolus 0.0002 0 0.0001 0.0001 0.0006 0 0.0064 0 0.0005 0 0.0001 0 – – – – – –
Nucleus 0.0015 0 0 0.0002 0.0171 0 0.0111 0.0001 0.0007 0 0.0004 0.0002 – – – – – –
Peroxisomes 0.0589 0.053 0.0543 0.0579 0.0794 0.0458 0.0151 0.0274 0.0463 0.0807 0.101 0.1165 – – – – – –
Punctate Nuclear 0.0017 0.0003 0.0004 0.0003 0.0012 0.0002 0.0029 0.0003 0.0024 0.0002 0.0019 0.0004 – – – – – –
Vacuole 0.0023 0.0005 0.0005 0.001 0.0018 0.0008 0.0149 0.0005 0.0031 0.0001 0.0108 0.0006 – – – – – –
Vacuole Periphery 0.0027 0.0005 0.0005 0.0018 0.0024 0.0008 0.0179 0.0004 0.001 0.0002 0.0143 0.0006 – – – – – –

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 31.8508 27.7948 27.1645 24.6636 23.5977 36.3808 24.8474 31.2275 32.2388 27.5371
Translational Efficiency 1.7994 1.8333 1.4574 1.7726 1.9102 1.27 1.494 1.2665 1.0605 1.3458

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
704 1403 477 1314 1325 1640 607 2787 2029 3043 1084 4101

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 914.58 1021.91 1348.84 1209.89 1001.32 978.39 1306.19 1312.28 971.22 998.46 1324.96 1279.47
Standard Deviation 115.68 175.36 163.88 158.69 140.37 154.03 151.01 169.36 138.62 165.64 158.23 172.75
Intensity Change Log 2 — 0.160087 0.560538 0.403695 — -0.033422 0.383462 0.390172 — 0.062194 0.470706 0.396643

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.004743 0.021501 0.026973 0.016320 0.007529 0.010242 0.016514 0.010049 0.006562 0.015433 0.021116 0.012058
Bud Neck 0.000149 0.000231 0.000848 0.001632 0.000255 0.000109 0.000699 0.001102 0.000218 0.000165 0.000764 0.001272
Bud Site 0.001036 0.002034 0.010846 0.045551 0.002912 0.001107 0.015783 0.017281 0.002261 0.001534 0.013611 0.026339
Cell Periphery 0.000020 0.000041 0.000125 0.000135 0.000044 0.000020 0.000068 0.000074 0.000036 0.000030 0.000093 0.000094
Cytoplasm 0.000542 0.001178 0.006201 0.129312* 0.002078 0.001373 0.013504 0.092779 0.001545 0.001283 0.010290 0.104485*
Cytoplasmic Foci 0.346820* 0.385785* 0.339580* 0.369963* 0.440752* 0.379679* 0.414946* 0.291416* 0.408161* 0.382495* 0.381782* 0.316584*
Eisosomes 0.000033 0.000059 0.000062 0.000035 0.000036 0.000038 0.000035 0.000032 0.000035 0.000048 0.000047 0.000033
Endoplasmic Reticulum 0.000208 0.000307 0.001667 0.000351 0.000329 0.000096 0.000575 0.000174 0.000287 0.000193 0.001055 0.000231
Endosome 0.159065 0.180087 0.150241 0.137330 0.125887 0.182654 0.178694 0.216981* 0.137398 0.181471 0.166174 0.191460
Golgi 0.387557* 0.344848* 0.402428* 0.176768* 0.369057* 0.360299* 0.273228* 0.270959* 0.375476* 0.353175* 0.330081* 0.240780*
Lipid Particles 0.014542 0.032216 0.016370 0.007755 0.022050 0.018510 0.010614 0.003250 0.019445 0.024829 0.013147 0.004694
Mitochondria 0.004570 0.000778 0.000056 0.002599 0.001685 0.001864 0.000164 0.004262 0.002686 0.001364 0.000116 0.003729
Mitotic Spindle 0.001693 0.002712 0.019506 0.040337 0.007977 0.001918 0.041071 0.034643 0.005797 0.002284 0.031582 0.036467
None 0.000008 0.000016 0.000009 0.000024 0.000024 0.000008 0.000006 0.000101 0.000018 0.000012 0.000007 0.000076
Nuclear Periphery 0.000007 0.000010 0.000074 0.000076 0.000021 0.000007 0.000070 0.000051 0.000017 0.000008 0.000072 0.000059
Nuclear Periphery Foci 0.000037 0.001008 0.002112 0.000881 0.000355 0.000136 0.001662 0.000260 0.000245 0.000538 0.001860 0.000459
Nucleolus 0.000005 0.000012 0.000003 0.000040 0.000015 0.000108 0.000006 0.000042 0.000012 0.000064 0.000004 0.000041
Nucleus 0.000006 0.000012 0.000025 0.000221 0.000031 0.000442 0.000037 0.000348 0.000022 0.000244 0.000032 0.000308
Peroxisomes 0.077730 0.025320 0.007087 0.027150 0.016662 0.040003 0.010230 0.023475 0.037851 0.033233 0.008847 0.024653
Vacuole 0.000480 0.001223 0.014354 0.037360 0.001368 0.000802 0.019952 0.027645 0.001060 0.000996 0.017489 0.030758
Vacuole Periphery 0.000749 0.000622 0.001435 0.006160 0.000933 0.000584 0.002144 0.005074 0.000869 0.000602 0.001832 0.005422

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -6.69 -5.51 -4.80 1.88 2.54 -1.68 -2.95 -1.46 0.23 2.15 -6.09 -5.89 -4.00 2.29 3.65
Bud Neck -1.58 -5.15 -12.89 -12.23 -5.19 2.72 -4.70 -10.31 -14.36 -4.17 1.30 -6.75 -15.82 -18.28 -6.11
Bud Site -2.12 -6.87 -17.77 -17.46 -12.36 2.17 -7.06 -11.28 -15.71 -0.91 1.27 -9.24 -20.20 -22.90 -8.45
Cell Periphery -3.47 -5.04 -10.77 -8.61 -0.56 3.79 -2.35 -1.91 -3.63 -0.38 1.24 -5.22 -5.12 -5.92 -0.12
Cytoplasm -4.38 -4.09 -21.75 -21.65 -20.20 1.05 -5.43 -25.85 -26.02 -19.43 0.60 -6.55 -33.67 -33.77 -28.26
Cytoplasmic Foci -4.60 0.76 -1.88 2.91 -2.43 8.89 2.90 24.52 16.04 14.84 4.74 3.82 18.40 15.37 10.41
Eisosomes -6.64 -4.55 -0.41 6.32 4.35 -0.78 0.31 0.85 1.32 0.57 -5.28 -3.40 0.67 4.20 3.23
Endoplasmic Reticulum -0.57 -4.13 -0.85 -0.59 4.16 4.25 -2.35 1.61 -0.90 3.26 1.28 -4.78 0.65 -0.56 5.24
Endosome -3.21 1.21 3.91 8.89 2.60 -11.85 -7.92 -18.01 -6.29 -5.26 -11.48 -5.86 -12.81 -1.86 -4.67
Golgi 4.52 -0.98 23.77 22.56 19.05* 1.16 9.02 14.46 13.85 0.69 3.80 5.50 24.52 22.52 11.47
Lipid Particles -5.01 -0.66 2.63 8.14 3.34 1.24 4.18 8.29 8.00 4.84 -2.30 2.90 8.63 11.82 6.14
Mitochondria 4.38 5.49 1.70 -1.79 -2.66 -0.23 4.04 -4.32 -2.83 -8.90 2.43 6.82 -1.70 -3.96 -8.34
Mitotic Spindle -1.51 -4.74 -11.77 -11.41 -4.40 4.07 -5.96 -10.92 -15.21 0.68 3.49 -7.27 -15.70 -18.95 -1.82
None -1.25 -0.48 -4.72 -1.41 -4.91 1.37 1.68 -2.71 -3.40 -3.51 0.77 1.59 -3.03 -3.47 -3.78
Nuclear Periphery -1.20 -6.11 -2.77 -2.69 -0.63 3.83 -4.38 -5.23 -8.02 1.15 3.29 -6.97 -4.71 -5.50 0.25
Nuclear Periphery Foci -1.57 -4.70 -5.77 -0.09 2.27 2.96 -3.76 0.90 -2.95 3.99 -1.02 -5.85 -3.74 0.01 4.80
Nucleolus -0.94 0.87 -5.71 -3.85 -6.87 -0.89 1.95 -5.61 0.59 -10.86 -0.92 2.26 -7.87 0.31 -13.07
Nucleus -1.23 -3.04 -7.99 -7.78 -7.25 -0.95 -0.86 -4.32 0.15 -4.19 -0.95 -1.60 -5.82 -0.42 -5.59
Peroxisomes 8.07 11.13 7.94 -0.54 -8.53 -7.54 2.96 -3.28 5.71 -6.67 1.55 10.81 5.06 4.44 -10.54
Vacuole -3.33 -6.81 -17.49 -17.12 -8.34 3.06 -8.46 -19.02 -19.40 -3.59 0.38 -10.78 -25.61 -25.63 -7.75
Vacuole Periphery 0.25 -1.39 -5.88 -6.99 -5.92 1.68 -3.64 -12.33 -12.72 -6.67 1.23 -3.69 -12.15 -13.98 -9.51
Standard name
Human Ortholog
Description Golgi matrix protein; involved in the structural organization of the cis-Golgi; interacts genetically with COG3 and USO1
Localization
Cell Percentages Golgi (16%), endosome (5%), mixed (28%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Rud3

Rud3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rud3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available