Standard name | |
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Human Ortholog | |
Description | Golgi matrix protein; involved in the structural organization of the cis-Golgi; interacts genetically with COG3 and USO1 |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 59 | 7 | 5 | 6 | 12 | 7 | 5 | 7 | 7 | 0 | 4 | 7 | 10 | 19 | 8 | 21 | 18 | 20 | 22 | 9 | 3 | 21 | 27 | 30 |
Bud | 21 | 1 | 1 | 0 | 4 | 3 | 7 | 11 | 11 | 7 | 12 | 19 | 5 | 17 | 8 | 4 | 2 | 1 | 2 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 4 | 0 | 3 | 3 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 8 | 8 | 10 | 4 | 11 | 7 | 16 | 13 | 8 | 10 | 8 | 12 | 7 | 11 | 21 | 22 | 8 | 5 | 1 | 4 | 0 | 1 | 2 | 3 |
Endoplasmic Reticulum | 12 | 4 | 2 | 1 | 4 | 4 | 5 | 9 | 7 | 1 | 0 | 4 | 6 | 1 | 6 | 3 | 2 | 1 | 0 | 1 | 1 | 1 | 1 | 5 |
Endosome | 95 | 2 | 7 | 2 | 20 | 13 | 36 | 32 | 6 | 5 | 7 | 7 | 19 | 36 | 82 | 37 | 15 | 11 | 78 | 16 | 21 | 25 | 35 | 31 |
Golgi | 157 | 31 | 15 | 21 | 32 | 9 | 10 | 8 | 3 | 7 | 4 | 6 | 103 | 165 | 145 | 75 | 65 | 46 | 298 | 49 | 35 | 104 | 168 | 186 |
Mitochondria | 281 | 48 | 39 | 34 | 57 | 64 | 124 | 134 | 86 | 111 | 103 | 184 | 75 | 103 | 87 | 12 | 14 | 9 | 6 | 1 | 3 | 2 | 3 | 3 |
Nucleus | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 1 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 1 | 1 | 4 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 12 | 0 | 0 | 1 | 1 | 3 | 8 | 7 | 5 | 2 | 4 | 3 | 4 | 2 | 12 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 7 | 0 | 0 | 2 | 0 | 0 | 9 | 9 | 3 | 7 | 2 | 7 | 8 | 14 | 10 | 2 | 0 | 1 | 27 | 1 | 5 | 10 | 11 | 14 |
SpindlePole | 11 | 0 | 0 | 0 | 4 | 3 | 9 | 20 | 5 | 10 | 8 | 10 | 3 | 1 | 8 | 9 | 14 | 6 | 5 | 0 | 0 | 1 | 1 | 0 |
Vac/Vac Membrane | 3 | 4 | 0 | 0 | 2 | 0 | 4 | 3 | 0 | 2 | 0 | 1 | 2 | 5 | 3 | 7 | 1 | 8 | 1 | 4 | 0 | 0 | 1 | 0 |
Unique Cell Count | 420 | 70 | 55 | 49 | 108 | 93 | 181 | 204 | 109 | 135 | 125 | 221 | 161 | 260 | 292 | 154 | 100 | 77 | 447 | 90 | 75 | 175 | 258 | 280 |
Labelled Cell Count | 668 | 105 | 81 | 72 | 147 | 114 | 236 | 257 | 145 | 166 | 161 | 264 | 243 | 374 | 391 | 194 | 141 | 108 | 447 | 90 | 75 | 175 | 258 | 280 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 9.2 | 9.8 | 7.7 | 8.1 | 7.7 | 6.9 | 5.9 | 6.1 | 5.8 | 5.5 | 5.6 | 5.4 | 10.4 | 10.5 | 9.9 | 11.1 | 12.7 | 13.7 | 9.9 | 10.1 | 10.7 |
Std Deviation (1e-4) | 1.2 | 1.5 | 1.4 | 1.2 | 1.2 | 1.1 | 1.1 | 1.2 | 1.0 | 1.0 | 1.4 | 1.1 | 1.7 | 1.6 | 1.4 | 2.0 | 2.4 | 2.6 | 1.4 | 1.7 | 1.7 |
Intensity Change (Log2) | – | – | – | 0.07 | 0.0 | -0.15 | -0.39 | -0.33 | -0.4 | -0.49 | -0.46 | -0.51 | 0.44 | 0.45 | 0.36 | 0.53 | 0.72 | 0.83 | 0.37 | 0.39 | 0.47 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0.5 | 0.4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.9 | 1.5 | 2.4 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -1.5 | -1.4 | -2.0 | -1.9 | 0 | -2.1 | -2.2 | -2.4 | 0 | 0 | 0 | 0 | -0.7 | -1.9 | -2.1 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0.9 | 0.2 | 1.2 | 0.5 | 0 | 0 | 0 | 0 | -0.2 | 0.2 | 2.4 | 1.8 | 0.4 | 0.3 |
Golgi | 1.7 | 0.3 | -2.8 | -4.6 | 0 | -4.7 | -4.3 | -4.8 | 0 | 4.7 | 4.9 | 3.1 | 2.8 | 4.5 | 3.7 |
Mitochondria | -0.2 | -2.2 | -0.3 | -0.3 | -0.7 | 1.1 | 1.7 | 1.7 | 2.1 | -3.1 | -4.2 | -5.8 | -9.4 | -7.1 | -7.0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Actin | 0.0343 | 0.0161 | 0.0585 | 0.0174 | 0.0461 | 0.023 | 0.0184 | 0.1583 | 0.0415 | 0.0804 | 0.0025 | 0.0435 | – | – | – | – | – | – |
Bud | 0.0004 | 0.0002 | 0.0002 | 0.0002 | 0.0007 | 0.0002 | 0.0004 | 0.0008 | 0.0002 | 0.0002 | 0.0001 | 0.0004 | – | – | – | – | – | – |
Bud Neck | 0.001 | 0.0004 | 0.0006 | 0.0004 | 0.0011 | 0.0008 | 0.0005 | 0.0009 | 0.0004 | 0.0012 | 0.0002 | 0.0006 | – | – | – | – | – | – |
Bud Periphery | 0.0004 | 0.0001 | 0.0002 | 0.0004 | 0.0005 | 0.0002 | 0.0005 | 0.0005 | 0.0001 | 0.0002 | 0.0001 | 0.0006 | – | – | – | – | – | – |
Bud Site | 0.0017 | 0.0006 | 0.0016 | 0.0004 | 0.0011 | 0.0004 | 0.001 | 0.0585 | 0.0017 | 0.0005 | 0.0002 | 0.0004 | – | – | – | – | – | – |
Cell Periphery | 0.0001 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0005 | 0.0001 | 0 | 0 | 0.0001 | – | – | – | – | – | – |
Cytoplasm | 0.0094 | 0.0075 | 0.0022 | 0.0013 | 0.0054 | 0.0027 | 0.0114 | 0.008 | 0.0246 | 0.0013 | 0.0125 | 0.0329 | – | – | – | – | – | – |
Cytoplasmic Foci | 0.1903 | 0.2747 | 0.1844 | 0.1476 | 0.1486 | 0.1546 | 0.1362 | 0.1898 | 0.2203 | 0.2711 | 0.1943 | 0.2334 | – | – | – | – | – | – |
Eisosomes | 0.0001 | 0 | 0.0001 | 0.0003 | 0.0001 | 0 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0 | 0.0002 | – | – | – | – | – | – |
Endoplasmic Reticulum | 0.004 | 0.0005 | 0.0004 | 0.0024 | 0.0008 | 0.0006 | 0.0076 | 0.0008 | 0.0022 | 0.0001 | 0.0044 | 0.0026 | – | – | – | – | – | – |
Endosome | 0.2246 | 0.2184 | 0.1503 | 0.1811 | 0.1878 | 0.1593 | 0.2705 | 0.1795 | 0.2205 | 0.1344 | 0.3082 | 0.1296 | – | – | – | – | – | – |
Golgi | 0.4223 | 0.4152 | 0.5201 | 0.5291 | 0.3696 | 0.5804 | 0.3209 | 0.3665 | 0.3984 | 0.4133 | 0.3105 | 0.4177 | – | – | – | – | – | – |
Lipid Particles | 0.0249 | 0.0089 | 0.013 | 0.0234 | 0.0541 | 0.0152 | 0.0122 | 0.0043 | 0.0271 | 0.0131 | 0.0173 | 0.0074 | – | – | – | – | – | – |
Mitochondria | 0.0089 | 0.0029 | 0.0124 | 0.0308 | 0.0574 | 0.0147 | 0.0152 | 0.0022 | 0.0021 | 0.0028 | 0.0038 | 0.0095 | – | – | – | – | – | – |
None | 0.0022 | 0.0001 | 0.0002 | 0.0008 | 0.0005 | 0.0001 | 0.0805 | 0.0002 | 0.0056 | 0.0001 | 0.0136 | 0.0019 | – | – | – | – | – | – |
Nuclear Periphery | 0.0081 | 0.0001 | 0.0001 | 0.0031 | 0.0238 | 0.0001 | 0.0562 | 0.0001 | 0.0012 | 0 | 0.0038 | 0.0008 | – | – | – | – | – | – |
Nucleolus | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0006 | 0 | 0.0064 | 0 | 0.0005 | 0 | 0.0001 | 0 | – | – | – | – | – | – |
Nucleus | 0.0015 | 0 | 0 | 0.0002 | 0.0171 | 0 | 0.0111 | 0.0001 | 0.0007 | 0 | 0.0004 | 0.0002 | – | – | – | – | – | – |
Peroxisomes | 0.0589 | 0.053 | 0.0543 | 0.0579 | 0.0794 | 0.0458 | 0.0151 | 0.0274 | 0.0463 | 0.0807 | 0.101 | 0.1165 | – | – | – | – | – | – |
Punctate Nuclear | 0.0017 | 0.0003 | 0.0004 | 0.0003 | 0.0012 | 0.0002 | 0.0029 | 0.0003 | 0.0024 | 0.0002 | 0.0019 | 0.0004 | – | – | – | – | – | – |
Vacuole | 0.0023 | 0.0005 | 0.0005 | 0.001 | 0.0018 | 0.0008 | 0.0149 | 0.0005 | 0.0031 | 0.0001 | 0.0108 | 0.0006 | – | – | – | – | – | – |
Vacuole Periphery | 0.0027 | 0.0005 | 0.0005 | 0.0018 | 0.0024 | 0.0008 | 0.0179 | 0.0004 | 0.001 | 0.0002 | 0.0143 | 0.0006 | – | – | – | – | – | – |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 31.8508 | 27.7948 | 27.1645 | 24.6636 | 23.5977 | 36.3808 | 24.8474 | 31.2275 | 32.2388 | 27.5371 |
Translational Efficiency | 1.7994 | 1.8333 | 1.4574 | 1.7726 | 1.9102 | 1.27 | 1.494 | 1.2665 | 1.0605 | 1.3458 |
Dataset | Hit |
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Protein Concentration | – |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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