Standard name
Human Ortholog
Description Mitochondrial protein required for iron-sulfur protein synthesis; performs scaffolding function during Fe/S cluster assembly; involved in Fe-S cluster assembly for both mitochondrial and cytosolic proteins; protein abundance increases under DNA replication stress; ISU2 has a paralog, ISU1, that arose from the whole genome duplication; isu1 isu2 double mutant is inviable; human homolog ISCU implicated in mitochondrial myopathy, can complement isu1 isu2 double mutant
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – 0.06 0 0 0 0 0.08 0.12
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – 0.12 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Golgi 0 0.05 0 0 0 0 – 0 0 0 0 0 0.05 0 0.12 – – 0.65 0.07 0.09 0.07 0.12 0.18 0.18
Mitochondria 0.92 0.86 0.96 0.96 0.87 0.91 – 0.84 0.89 0.89 0.91 0.92 0.95 0.95 0.91 – – 0.12 0.83 0.75 0.83 0.7 0.58 0.57
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0.06 0 0 0 – – 0 0 0 0 0 0 0
Nucleolus 0.16 0.11 0.14 0.09 0.15 0.15 – 0.23 0.2 0.16 0.13 0.17 0.1 0 0 – – 0.18 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – 0 0 0 0 0.05 0.06 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – 0.12 0 0 0 0 0 0
Vac/Vac Membrane 0.1 0.1 0 0 0.05 0 – 0 0 0 0 0 0 0 0.09 – – 0.12 0 0.06 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 2 2 0 0 1 – 0 0 1 1 0 2 6 7 – – 1 9 4 5 3 11 29
Bud 2 0 6 4 1 4 – 4 1 3 5 3 0 1 0 – – 0 0 0 0 0 1 1
Bud Neck 0 0 0 0 0 0 – 0 0 1 0 0 0 0 0 – – 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 2 0 0 0 – – 0 – – – – – –
Cell Periphery 3 2 2 0 1 1 – 3 1 3 2 0 1 2 2 – – 0 0 0 0 0 0 0
Cytoplasm 2 1 0 6 11 5 – 9 12 5 12 8 1 8 5 – – 2 0 0 0 0 0 2
Endoplasmic Reticulum 1 2 0 0 4 2 – 3 2 4 6 1 0 0 0 – – 0 0 0 0 0 0 0
Endosome 0 0 0 1 0 1 – 0 0 0 0 1 0 0 0 – – 0 2 0 1 1 1 1
Golgi 8 7 0 1 6 2 – 5 1 2 3 1 10 5 27 – – 11 28 14 10 13 28 44
Mitochondria 336 125 155 199 225 275 – 358 343 313 395 393 191 178 203 – – 2 321 120 131 73 91 141
Nucleus 3 0 0 0 0 2 – 2 1 1 0 1 0 1 0 – – 0 0 1 0 0 0 1
Nuclear Periphery 4 1 2 0 2 9 – 13 17 11 10 25 2 0 0 – – 0 2 0 0 0 1 1
Nucleolus 57 16 23 18 39 45 – 97 75 55 55 71 20 8 7 – – 3 7 4 1 2 1 2
Peroxisomes 1 1 1 2 3 2 – 1 2 1 4 3 1 0 0 – – 0 3 0 5 5 9 9
SpindlePole 0 1 0 0 0 0 – 2 1 1 0 0 0 0 0 – – 2 0 1 0 1 1 2
Vac/Vac Membrane 37 15 4 0 12 8 – 11 8 8 3 1 8 4 21 – – 2 10 10 0 1 5 8
Unique Cell Count 367 145 161 208 260 301 428 384 351 432 425 200 188 222 17 388 160 159 105 157 249
Labelled Cell Count 455 173 195 231 304 357 508 464 409 496 510 236 213 272 23 388 160 159 105 157 249


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 28.4 36.6 20.2 30.6 31.4 26.4 – 24.5 23.6 22.1 21.9 20.5 43.2 42.0 45.6 – – 42.9 27.5 33.6 33.7
Std Deviation (1e-4) 8.2 10.5 6.4 11.2 10.1 8.5 – 8.8 7.6 7.0 7.0 6.4 12.4 12.9 16.1 – – 21.0 9.1 10.5 12.7
Intensity Change (Log2) – – – 0.6 0.64 0.39 – 0.28 0.23 0.13 0.12 0.02 1.1 1.06 1.18 – – 1.09 0.45 0.74 0.74


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 – – 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 – – 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 – – 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 – – 0
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 – – 0
Cytoplasm 0 0 0 – 0 0 0 0 0 0 0 0 – – 0
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0 – – 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 – – 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 4.6 – – 0
Mitochondria -0.3 -3.3 -2.0 – -4.1 -2.6 -2.7 -2.0 -1.7 -0.4 -0.7 -1.9 – – -10.3
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 – – 0
Nuclear Periphery 0 0 0 – 0 1.8 0 0 2.4 0 0 0 – – 0
Nucleolus -1.7 0.2 0.2 – 2.2 1.5 0.4 -0.5 0.7 -1.2 -3.3 -4.0 – – 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 – – 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 – – 0
Vacuole 0 1.1 0 – 0 0 0 0 0 0.8 0 2.7 – – 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 27.5166 26.3122 26.0442 28.944 24.7859 27.1681 24.0516 28.6761 25.2193 18.6705 22.2278 27.4609 31.8006 29.4133 29.364 27.7142 26.6566 29.2783
Actin 0.023 0.0036 0.0092 0.0004 0.0019 0.0006 0.0202 0.0128 0.0106 0.0051 0.0004 0.0004 0.0064 0.0058 0.0006 0.0001 0.0002 0.0004
Bud 0.0002 0.0001 0.0001 0 0.0001 0 0.0002 0.0002 0.0002 0.002 0 0 0 0 0 0 0 0
Bud Neck 0.0002 0.0001 0.0001 0 0 0 0.0027 0.0001 0.0004 0.0008 0 0 0 0 0 0 0 0
Bud Periphery 0.0005 0.0003 0.0002 0.0001 0.0001 0.0001 0.0003 0.0003 0.0003 0.0029 0.0002 0 0 0.0001 0.0001 0 0 0
Bud Site 0.0007 0.0002 0.0003 0 0.0001 0.0001 0.0011 0.0005 0.0016 0.0062 0 0 0.0001 0.0001 0.0001 0 0.0001 0
Cell Periphery 0.0002 0.0005 0.0002 0.0001 0 0 0.0005 0.0004 0.0003 0.0005 0.0002 0.0001 0.0001 0.0002 0.0001 0 0.0001 0
Cytoplasm 0.0003 0 0.0003 0 0 0 0.0008 0 0.0001 0.0005 0 0 0.0001 0 0.0005 0 0.0001 0
Cytoplasmic Foci 0.0069 0.0002 0.0083 0 0.0066 0.0013 0.0143 0.0003 0.0036 0.012 0.0001 0.0059 0.0009 0.0002 0.0028 0 0.0054 0.0003
Eisosomes 0.0007 0.0003 0.0004 0.0006 0.0002 0.0002 0.0013 0.0023 0.0013 0.0003 0.0003 0.0003 0.0003 0.0002 0.0003 0.0001 0.0001 0.0004
Endoplasmic Reticulum 0.0011 0 0.0018 0 0.0002 0.0001 0.0015 0 0 0.0005 0 0 0.0001 0 0.0005 0 0 0
Endosome 0.0031 0.0015 0.0029 0 0.0017 0.0007 0.013 0.0007 0.0013 0.0231 0.0001 0.0003 0.0005 0.0004 0.0007 0.0001 0.0001 0.0005
Golgi 0.0059 0.009 0.0033 0.0001 0.0034 0.0013 0.008 0.0025 0.0054 0.021 0.0002 0.0019 0.0009 0.0013 0.001 0.0004 0.0002 0.0039
Lipid Particles 0.0083 0.0041 0.0207 0.0002 0.0538 0.0068 0.0178 0.004 0.0076 0.045 0.0014 0.024 0.0177 0.011 0.0123 0.0005 0.0046 0.0041
Mitochondria 0.9256 0.956 0.9273 0.9967 0.9125 0.9841 0.893 0.96 0.9569 0.8367 0.9707 0.9499 0.9589 0.9551 0.9701 0.9978 0.944 0.9819
None 0.0005 0 0.0005 0 0.0001 0.0001 0.0004 0 0.0001 0.0004 0 0 0.0006 0 0.0009 0 0.0003 0
Nuclear Periphery 0.002 0.0001 0.0014 0 0.0004 0.0001 0.0033 0.0001 0 0.0019 0 0 0.0001 0 0.0004 0 0.0002 0
Nucleolus 0.0005 0.001 0.0038 0 0.0001 0.0002 0.0007 0.001 0.0001 0.0018 0.0002 0.0006 0.002 0.0003 0.0004 0 0.0377 0
Nucleus 0.0003 0.0003 0.0005 0 0.0001 0.0001 0.0003 0.0001 0 0.0013 0.0001 0.0001 0.0001 0.0001 0.0001 0 0.0027 0
Peroxisomes 0.0096 0.001 0.0035 0.0003 0.0164 0.001 0.0096 0.0022 0.0022 0.0093 0.0011 0.0057 0.0044 0.0062 0.0009 0.0003 0.0006 0.0069
Punctate Nuclear 0.0009 0 0.0006 0 0.0009 0.0001 0.0007 0 0.0001 0.0007 0 0 0.0002 0 0.0007 0 0.0027 0
Vacuole 0.0011 0.0079 0.0016 0.0001 0.0002 0.0003 0.0034 0.0022 0.0009 0.0074 0.0024 0.0005 0.0006 0.0029 0.0005 0 0.0002 0.0001
Vacuole Periphery 0.0084 0.0138 0.0129 0.0013 0.0011 0.0029 0.0068 0.0105 0.007 0.0205 0.0225 0.0101 0.0059 0.0161 0.007 0.0006 0.0008 0.0015

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 43.3397 71.2034 45.066 56.4058 62.2666 60.8761 64.0532 66.4552 48.1171 57.0433
Translational Efficiency 2.3923 2.1095 2.5079 1.787 1.5587 1.5906 1.9046 2.0318 2.4084 1.9758

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2262 1617 937 1367 2075 1872 895 1643 4337 3489 1832 3010

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 2213.99 2634.27 5053.96 3264.28 3262.24 2360.30 2843.38 3024.41 2715.52 2487.27 3974.01 3133.35
Standard Deviation 694.56 807.70 1224.27 995.57 1032.65 667.53 1069.80 847.66 1017.84 748.40 1595.85 925.54
Intensity Change Log 2 — 0.250755 1.190766 0.560116 — -0.466893 -0.198256 -0.109209 — -0.132823 0.528184 0.199576

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.002848 0.005294 0.002752 0.008151 0.002138 0.003625 0.007509 0.004083 0.002509 0.004398 0.005076 0.005930
Bud Neck 0.000551 0.001157 0.001624 0.002219 0.000307 0.001260 0.011852 0.001997 0.000434 0.001212 0.006621 0.002098
Bud Site 0.000954 0.000782 0.000653 0.003191 0.000208 0.001208 0.040880 0.003172 0.000597 0.001010 0.020305 0.003181
Cell Periphery 0.000221 0.000097 0.000037 0.000531 0.000094 0.000071 0.008807 0.000359 0.000161 0.000083 0.004322 0.000437
Cytoplasm 0.000172 0.000342 0.000194 0.000317 0.000058 0.000276 0.003394 0.000500 0.000118 0.000306 0.001757 0.000417
Cytoplasmic Foci 0.011340 0.020448 0.036659 0.010657 0.007516 0.017718 0.026730 0.009578 0.009511 0.018983 0.031808 0.010068
Eisosomes 0.004464 0.003621 0.003111 0.003451 0.006791 0.001979 0.002118 0.001294 0.005577 0.002740 0.002626 0.002274
Endoplasmic Reticulum 0.000054 0.000094 0.000038 0.000412 0.000034 0.000064 0.000769 0.000152 0.000044 0.000078 0.000395 0.000270
Endosome 0.000294 0.000487 0.000171 0.001326 0.000086 0.000806 0.006612 0.001615 0.000194 0.000658 0.003318 0.001483
Golgi 0.003454 0.014205 0.008110 0.016587 0.002995 0.013025 0.010543 0.016920 0.003235 0.013572 0.009298 0.016769
Lipid Particles 0.003544 0.005944 0.007813 0.006103 0.003419 0.005781 0.008106 0.003720 0.003484 0.005857 0.007956 0.004802
Mitochondria 0.946247* 0.920224* 0.886953* 0.903630* 0.958648* 0.928532* 0.783484* 0.914377* 0.952180* 0.924682* 0.836404* 0.909496*
Mitotic Spindle 0.000071 0.000643 0.002407 0.002254 0.000044 0.000355 0.004568 0.000922 0.000058 0.000488 0.003463 0.001527
None 0.006242 0.003467 0.008980 0.005446 0.007009 0.004025 0.003998 0.004622 0.006609 0.003767 0.006546 0.004996
Nuclear Periphery 0.000164 0.000195 0.001843 0.000713 0.000187 0.000257 0.001156 0.000661 0.000175 0.000229 0.001507 0.000685
Nuclear Periphery Foci 0.000105 0.000173 0.000360 0.000218 0.000064 0.000203 0.000744 0.000281 0.000086 0.000189 0.000547 0.000253
Nucleolus 0.001014 0.001646 0.002277 0.001166 0.000614 0.001969 0.007141 0.001007 0.000823 0.001819 0.004653 0.001079
Nucleus 0.000123 0.000567 0.000076 0.000246 0.000045 0.000113 0.001383 0.000489 0.000085 0.000324 0.000714 0.000379
Peroxisomes 0.010638 0.014451 0.013465 0.004839 0.004664 0.007052 0.010288 0.004203 0.007780 0.010481 0.011913 0.004492
Vacuole 0.002602 0.002708 0.004575 0.008050 0.001140 0.005753 0.029540 0.009026 0.001903 0.004342 0.016771 0.008583
Vacuole Periphery 0.004895 0.003455 0.017904 0.020494 0.003939 0.005927 0.030378 0.021023 0.004438 0.004782 0.023998 0.020782

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.43 0.12 -4.39 -2.28 -4.80 -1.92 -4.75 -2.34 -0.49 2.76 -2.98 -3.68 -4.73 -2.00 -1.01
Bud Neck -2.68 -4.29 -4.10 -2.62 -1.48 -5.09 -8.88 -4.94 -2.10 7.26 -5.22 -9.39 -6.29 -3.31 6.35
Bud Site 0.36 0.73 -3.11 -3.81 -4.07 -3.09 -9.29 -4.37 -2.66 8.43 -1.64 -8.96 -5.16 -4.33 7.55
Cell Periphery 1.28 2.09 -2.07 -3.34 -3.95 0.82 -5.18 -2.98 -3.34 5.01 1.50 -5.02 -3.25 -4.69 4.66
Cytoplasm -2.04 -0.41 -2.14 0.16 -1.87 -1.69 -2.92 -3.47 -0.86 2.41 -2.33 -2.93 -3.95 -0.74 2.21
Cytoplasmic Foci -5.02 -7.88 0.17 4.71 7.71 -7.20 -6.94 -1.98 4.62 5.77 -8.28 -10.46 -1.00 6.60 9.64
Eisosomes 1.80 3.77 2.22 0.32 -0.81 11.37 9.83 13.25 4.53 3.12 9.25 10.00 11.11 1.83 1.47
Endoplasmic Reticulum -1.99 1.75 -2.41 -2.11 -2.52 -3.23 -6.80 -4.53 -3.35 5.52 -3.10 -6.53 -3.28 -2.77 1.39
Endosome -1.65 1.30 -3.32 -2.73 -3.87 -4.42 -7.84 -3.92 -1.95 5.39 -4.40 -7.50 -5.03 -3.10 3.73
Golgi -8.41 -5.06 -7.24 -1.11 -4.32 -7.50 -5.59 -7.68 -1.80 -2.93 -11.13 -7.49 -10.53 -2.09 -5.09
Lipid Particles -2.61 -3.95 -2.15 -0.12 1.11 -2.61 -3.55 -0.41 2.12 3.21 -3.70 -5.26 -1.93 1.25 3.15
Mitochondria 5.16 8.37 7.11 2.46 -1.85 7.02 15.69 8.81 2.58 -10.90 8.31 17.39 11.00 3.55 -9.84
Mitotic Spindle -1.71 -5.38 -2.75 -1.84 0.14 -3.60 -3.35 -3.32 -2.10 2.62 -2.63 -4.88 -3.81 -2.48 2.38
None 8.86 -4.99 1.83 -5.29 6.03 7.06 6.14 5.50 -1.95 -1.59 10.84 0.18 5.35 -5.16 4.32
Nuclear Periphery -1.28 -5.19 -6.11 -5.69 3.36 -1.90 -3.03 -4.15 -3.56 1.45 -2.41 -5.85 -6.86 -6.12 3.43
Nuclear Periphery Foci -1.57 -4.80 -3.42 -0.97 2.39 -5.06 -6.95 -4.54 -1.60 4.16 -4.11 -8.38 -5.51 -1.86 4.66
Nucleolus -1.19 -3.07 -0.75 0.81 2.49 -2.26 -4.09 -2.08 1.49 3.80 -2.45 -4.73 -1.80 1.67 4.33
Nucleus -1.50 0.85 -1.79 1.14 -2.88 -2.57 -3.87 -2.48 -2.20 1.69 -1.65 -3.67 -2.84 -0.22 1.03
Peroxisomes -2.10 -1.38 4.13 5.99 4.60 -2.43 -3.92 0.48 2.82 4.19 -2.64 -3.26 3.84 6.50 6.23
Vacuole -0.26 -3.18 -5.12 -4.89 -3.09 -6.31 -10.97 -7.70 -2.73 7.32 -5.31 -11.13 -9.01 -5.15 5.31
Vacuole Periphery 1.85 -6.05 -8.07 -8.90 -0.94 -2.67 -8.06 -9.80 -8.47 2.55 -0.63 -10.03 -12.60 -12.27 1.40
Standard name
Human Ortholog
Description Mitochondrial protein required for iron-sulfur protein synthesis; performs scaffolding function during Fe/S cluster assembly; involved in Fe-S cluster assembly for both mitochondrial and cytosolic proteins; protein abundance increases under DNA replication stress; ISU2 has a paralog, ISU1, that arose from the whole genome duplication; isu1 isu2 double mutant is inviable; human homolog ISCU implicated in mitochondrial myopathy, can complement isu1 isu2 double mutant
Localization
Cell Percentages mitochondrion (98%)
Cell Cycle Regulation No
Subcompartmental Group mito-3

Isu2

Isu2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Isu2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available