Standard name
Human Ortholog
Description Transcriptional modulator; involved in regulation of meiosis, silencing, and expression of RNR genes; involved in response to replication stress; contains WD repeats; relocalizes to the cytosol in response to hypoxia; WTM2 has a paralog, UME1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.07 0.05 0.07 0.11 0.07 0.05 0.09 0 0.11 0.12 0.13 0 0.1 0.05 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.1 0.12 0.16 0.29 0.34 0.31 0.33 0.39 0.35 0.35 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.95 0.96 0.96 0.9 0.82 0.83 0.74 0.69 0.75 0.73 0.67 0.78 0.84 0.83 0.79 0.83 0.8 0.83 0.88 0.92 0.93 0.89 0.83 0.73
Nuclear Periphery 0.1 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0.05 0.07 0.05 0.1 0.09 0.12 0.14 0.16 0.18 0.16 0 0 0 0.12 0 0.08 0 0 0 0 0.06 0.06
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0.05 0.07 0.14
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 1 0 0 1 2 0 0 0 0 0 0 0 0 0 0 1 0 0 2 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 1 0 2 3 3 4 6 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2 2 1 3 8 6 12 29 12 10 9 6 33 42 54 5 14 6 4 1 0 0 2 2
Endoplasmic Reticulum 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 1
Endosome 0 2 0 0 0 0 0 0 0 0 0 0 2 2 5 1 1 0 0 1 0 0 1 2
Golgi 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 1 0 1 0 0 0 0 0
Mitochondria 0 5 10 10 19 36 63 83 58 77 37 53 2 2 2 1 3 2 0 0 0 1 1 3
Nucleus 78 258 97 72 96 103 137 184 132 147 70 118 243 287 341 151 114 96 103 257 93 259 249 195
Nuclear Periphery 8 5 1 5 2 2 2 6 1 6 3 4 3 0 7 3 0 1 0 0 0 0 0 2
Nucleolus 1 3 5 6 6 12 16 33 24 32 19 24 6 10 17 22 4 9 1 3 1 8 17 15
Peroxisomes 0 0 1 0 0 0 0 0 0 0 0 0 1 6 10 0 0 0 0 0 1 0 0 0
SpindlePole 0 0 0 0 0 0 0 1 0 0 0 0 4 1 7 0 1 0 0 0 0 2 0 0
Vac/Vac Membrane 1 5 0 1 8 2 4 8 2 1 1 6 4 3 13 8 10 4 1 9 3 13 20 37
Unique Cell Count 82 270 101 80 117 124 184 267 175 200 105 152 288 345 430 182 142 115 118 279 100 290 300 267
Labelled Cell Count 90 280 115 97 140 162 236 348 234 276 143 217 299 353 456 192 149 119 118 279 100 290 300 267


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.5 8.0 6.1 5.9 6.1 5.5 5.3 5.5 5.6 5.1 5.3 5.2 9.2 8.5 8.3 12.9 16.5 18.0 8.1 8.5 9.7
Std Deviation (1e-4) 1.6 1.6 1.3 1.3 1.8 1.2 1.6 1.4 1.6 1.6 1.8 1.5 3.0 2.5 2.5 3.5 5.3 5.0 1.7 2.3 2.4
Intensity Change (Log2) – – – -0.05 -0.01 -0.15 -0.21 -0.16 -0.12 -0.27 -0.19 -0.24 0.59 0.49 0.45 1.09 1.44 1.56 0.41 0.48 0.67


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 3.1 0 0 0 0 3.2 3.3 3.4 0 2.8 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.6 1.4 3.5 4.5 4.2 4.3 5.2 4.3 4.5 0 0 0 0 -2.7 -2.6
Nucleus -1.6 -3.2 -3.1 -4.5 -5.5 -4.4 -4.7 -5.4 -4.0 -3.0 -3.3 -4.0 -3.2 -3.6 -3.0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0.1 1.3 1.2 2.1 2.3 2.8 2.9 2.7 0 0 0 2.0 0 0.9
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.3637 5.1314 4.648 4.6768 4.2464 4.8547 4.9703 5.7233 4.4403 3.4224 4.2461 4.6498 7.11 7.1854 6.8808 6.7369 5.9056 6.8561
Actin 0.0112 0 0.0013 0 0.0001 0.0051 0.0019 0.0002 0.0175 0 0.0001 0.0072 0.009 0.0001 0.0157 0.023 0.009 0.0031
Bud 0.0003 0.0001 0 0 0 0.0001 0.0003 0 0.0002 0 0.0001 0.0001 0.0003 0.0001 0.0004 0.0001 0.0007 0.0001
Bud Neck 0.0014 0.0002 0.0001 0.0001 0.0006 0.0008 0.0017 0.0001 0.0001 0 0.0006 0.0008 0.0016 0.0001 0.0004 0.0007 0.001 0.0004
Bud Periphery 0.0007 0 0 0 0.0001 0.0005 0.0014 0 0.0004 0 0.0001 0.0001 0.0008 0.0001 0.0006 0.0003 0.0015 0.0006
Bud Site 0.0011 0.0015 0.0005 0 0 0.0002 0.0009 0.0001 0.0006 0 0.0004 0.0002 0.0018 0.0001 0.006 0.0003 0.0034 0.0003
Cell Periphery 0.0004 0 0.0001 0 0 0.0001 0.0006 0 0.0001 0 0.0001 0 0.0005 0 0.0001 0.0001 0.0006 0.0001
Cytoplasm 0.0088 0.0015 0.0008 0.0002 0.0001 0.0001 0.0246 0.0014 0.0005 0 0.0006 0.0037 0.0014 0.0026 0.0052 0.0001 0.0004 0.0004
Cytoplasmic Foci 0.0031 0 0.0004 0 0 0.0008 0.0078 0 0.0065 0 0.0001 0.0061 0.0075 0 0.0111 0.0008 0.0119 0.0031
Eisosomes 0.0009 0 0 0 0 0 0.0001 0 0.0001 0 0 0 0.0001 0 0.0001 0.0001 0.0001 0
Endoplasmic Reticulum 0.003 0 0.0015 0 0.0001 0.0002 0.0096 0.0003 0.0001 0 0.0001 0.0031 0.0009 0 0.0006 0.0002 0.0003 0.0008
Endosome 0.0153 0 0.0026 0 0.0002 0.0018 0.0059 0.0002 0.0008 0 0.0002 0.0128 0.0134 0 0.0049 0.0009 0.011 0.0032
Golgi 0.0075 0 0.0006 0 0 0.0013 0.0007 0 0.0009 0 0 0.0042 0.0043 0 0.0032 0.0016 0.0119 0.0011
Lipid Particles 0.0087 0 0.0012 0 0 0.0025 0.0069 0 0.0046 0 0 0.0021 0.0094 0 0.0045 0.0007 0.0306 0.0023
Mitochondria 0.0155 0.0001 0.0079 0.0001 0.0005 0.0016 0.0018 0.0002 0.0251 0 0.0005 0.0013 0.0156 0.0001 0.0096 0.0204 0.0121 0.0086
None 0.0023 0 0.0002 0 0.0001 0.0001 0.049 0.0002 0.0003 0 0.0001 0.0002 0.0013 0 0.0021 0.0001 0.0003 0.0004
Nuclear Periphery 0.0153 0.0044 0.0025 0.0037 0.0099 0.0035 0.0095 0.0107 0.0051 0.0004 0.0015 0.002 0.0176 0.0025 0.0116 0.0029 0.0008 0.0141
Nucleolus 0.0138 0.0101 0.0059 0.0143 0.052 0.0436 0.0126 0.0057 0.0038 0.0018 0.0531 0.0216 0.0128 0.0055 0.0064 0.0042 0.033 0.0199
Nucleus 0.8766 0.9805 0.9707 0.9813 0.9337 0.9277 0.8549 0.9796 0.9201 0.9976 0.9397 0.926 0.861 0.985 0.9015 0.9405 0.8335 0.9325
Peroxisomes 0.0012 0 0.0001 0 0 0.0016 0.001 0 0.0105 0 0 0.0068 0.004 0 0.0114 0.0006 0.0311 0.0029
Punctate Nuclear 0.0066 0.0012 0.0025 0.0003 0.0014 0.0066 0.006 0.0006 0.0027 0.0001 0.0017 0.001 0.0297 0.0036 0.0028 0.0001 0.0016 0.0029
Vacuole 0.0041 0.0002 0.001 0 0.0004 0.001 0.0022 0.0003 0.0001 0 0.0005 0.0002 0.0052 0 0.0009 0.0005 0.0044 0.0011
Vacuole Periphery 0.0024 0.0001 0.0001 0 0.0007 0.0007 0.0006 0.0001 0.0001 0 0.0002 0.0002 0.0016 0 0.0007 0.0018 0.0009 0.002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 7.4661 18.2662 31.8005 29.7605 13.2464 25.9204 24.1517 28.7661 32.1585 17.674
Translational Efficiency 0.7118 0.4977 0.6811 0.6537 0.5248 0.3011 0.557 0.6255 0.5554 0.5022

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1643 1663 137 1577 209 1807 171 1201 1852 3470 308 2778

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 831.52 1008.09 1451.37 1077.91 1061.58 942.77 1303.53 1032.79 857.48 974.07 1369.29 1058.40
Standard Deviation 123.90 204.16 171.05 153.52 163.33 186.29 143.07 165.52 148.08 197.77 172.56 160.38
Intensity Change Log 2 — 0.277802 0.803592 0.374414 — -0.171235 0.296211 -0.039666 — 0.043360 0.541249 0.156971

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000326 0.000678 0.001141 0.001253 0.000645 0.000588 0.001338 0.002141 0.000362 0.000631 0.001251 0.001637
Bud Neck 0.002392 0.005371 0.008002 0.035239 0.001556 0.008325 0.010815 0.028053 0.002298 0.006910 0.009564 0.032132
Bud Site 0.000790 0.000731 0.000684 0.005814 0.000177 0.001852 0.001116 0.011291 0.000721 0.001315 0.000924 0.008182
Cell Periphery 0.000289 0.000287 0.000030 0.000114 0.000019 0.000268 0.000058 0.000196 0.000259 0.000277 0.000046 0.000149
Cytoplasm 0.003394 0.003852 0.000101 0.022083 0.000063 0.002613 0.000782 0.040596 0.003019 0.003207 0.000479 0.030087
Cytoplasmic Foci 0.000164 0.000208 0.000035 0.002397 0.000024 0.000445 0.000049 0.008992 0.000148 0.000331 0.000043 0.005248
Eisosomes 0.000030 0.000037 0.000067 0.000022 0.000031 0.000038 0.000049 0.000036 0.000030 0.000037 0.000057 0.000028
Endoplasmic Reticulum 0.006976 0.005516 0.002550 0.007656 0.003465 0.007627 0.007022 0.008046 0.006580 0.006615 0.005033 0.007824
Endosome 0.000411 0.000314 0.000046 0.005058 0.000044 0.000532 0.000199 0.009687 0.000370 0.000428 0.000131 0.007059
Golgi 0.000107 0.000167 0.000061 0.003027 0.000064 0.000119 0.000098 0.005819 0.000102 0.000142 0.000082 0.004234
Lipid Particles 0.000079 0.000085 0.000049 0.000359 0.000044 0.000320 0.000183 0.001025 0.000075 0.000207 0.000123 0.000647
Mitochondria 0.000498 0.000938 0.001748 0.006359 0.000680 0.001888 0.001526 0.007682 0.000518 0.001433 0.001625 0.006931
Mitotic Spindle 0.000026 0.000443 0.000025 0.144848* 0.000031 0.002926 0.000409 0.114375* 0.000027 0.001736 0.000238 0.131674*
None 0.005791 0.005256 0.000437 0.005153 0.001062 0.006007 0.001236 0.010843 0.005257 0.005647 0.000881 0.007613
Nuclear Periphery 0.000201 0.000279 0.000128 0.008494 0.000060 0.000876 0.000580 0.006417 0.000185 0.000590 0.000379 0.007596
Nuclear Periphery Foci 0.000210 0.000733 0.000023 0.004920 0.000099 0.001030 0.000371 0.008276 0.000198 0.000887 0.000216 0.006371
Nucleolus 0.015701 0.016347 0.019464 0.027051 0.056648 0.015824 0.018301 0.029975 0.020321 0.016074 0.018818 0.028315
Nucleus 0.961226* 0.956716* 0.962746* 0.707164* 0.933685* 0.945463* 0.951180* 0.679809* 0.958118* 0.950856* 0.956324* 0.695338*
Peroxisomes 0.000255 0.000260 0.001585 0.000529 0.001281 0.000360 0.000626 0.003744 0.000371 0.000312 0.001052 0.001919
Vacuole 0.000786 0.001157 0.000948 0.010045 0.000213 0.001987 0.003870 0.018124 0.000721 0.001589 0.002570 0.013538
Vacuole Periphery 0.000346 0.000625 0.000128 0.002415 0.000107 0.000913 0.000193 0.004874 0.000319 0.000775 0.000164 0.003478

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.14 -9.45 -11.20 -6.97 -2.95 0.60 -7.44 -5.91 -6.13 -0.89 -4.95 -12.26 -11.97 -9.32 -1.90
Bud Neck -7.11 -5.32 -23.99 -22.34 -17.72 -8.35 -8.97 -18.93 -15.00 -13.04 -9.60 -9.64 -30.27 -26.34 -22.05
Bud Site 0.07 -0.13 -11.82 -12.85 -12.83 -4.77 -7.65 -12.71 -10.50 -11.26 -1.76 -1.60 -16.04 -16.02 -16.74
Cell Periphery 0.01 3.85 2.27 2.89 -15.24 -6.20 -5.87 -13.55 2.59 -5.93 -0.30 3.40 1.86 3.95 -11.35
Cytoplasm -0.49 3.57 -11.37 -11.01 -15.37 -4.62 -3.68 -13.54 -12.21 -12.95 -0.32 2.74 -15.65 -16.64 -19.38
Cytoplasmic Foci -0.32 1.51 -3.90 -3.51 -4.74 -2.30 -3.13 -5.32 -4.52 -5.24 -1.42 1.16 -6.20 -5.44 -6.62
Eisosomes -2.16 -7.26 -2.83 -0.20 6.33 -2.52 -5.43 -6.61 -4.40 1.62 -2.92 -8.42 -5.54 -3.04 5.72
Endoplasmic Reticulum 2.75 6.15 -2.46 -5.72 -8.59 -9.49 -5.15 -10.06 -2.54 0.19 -0.15 -0.03 -4.46 -5.38 -2.84
Endosome 0.52 1.83 -6.42 -7.16 -7.83 -6.52 -4.15 -6.81 -6.03 -6.27 -0.43 0.77 -8.60 -8.99 -9.50
Golgi -0.80 1.03 -3.64 -3.39 -3.81 -2.49 -2.93 -4.05 -3.93 -3.86 -0.84 -0.30 -5.35 -5.21 -5.34
Lipid Particles -0.15 0.31 -10.98 -10.90 -12.92 -1.97 -3.88 -11.29 -4.48 -6.28 -1.63 -2.66 -14.03 -7.31 -10.10
Mitochondria -3.51 -8.21 -11.59 -10.50 -8.22 -3.04 -6.38 -7.19 -4.29 -5.36 -4.08 -9.80 -13.21 -9.28 -9.58
Mitotic Spindle -1.82 -0.82 -22.02 -21.96* -22.02 -2.93 -1.96 -19.02 -18.58 -18.76 -3.25 -1.97 -29.10 -28.69* -28.87*
None 0.45 5.78 -0.81 -1.62 -17.34 -4.85 -0.68 -7.59 -1.47 -7.31 -0.37 5.27 -2.08 -2.01 -13.93
Nuclear Periphery -1.57 -0.55 -16.46 -16.33 -15.92 -3.95 -4.53 -12.61 -10.71 -10.33 -3.64 -3.69 -20.54 -19.22 -18.60
Nuclear Periphery Foci -2.01 0.30 -8.60 -7.94 -8.60 -2.03 -1.31 -6.99 -6.29 -4.96 -2.56 -1.20 -10.93 -9.95 -8.45
Nucleolus -0.47 -1.30 -12.81 -12.76 -9.28 7.22 5.51 -1.29 -11.91 -8.20 3.18 0.37 -14.98 -17.47 -11.97
Nucleus 1.55 0.49 38.51 37.93 35.75 -1.71 -0.38 29.46 33.30 28.29 2.74 2.35 50.92 50.16 43.61
Peroxisomes 0.07 -4.97 -4.30 -6.60 3.13 5.28 3.59 -1.99 -3.98 -3.35 0.62 -4.67 -4.65 -5.14 -1.46
Vacuole -1.20 -1.19 -11.28 -11.02 -9.43 -5.42 -3.54 -10.89 -10.14 -5.98 -3.31 -3.50 -15.44 -14.81 -9.35
Vacuole Periphery -1.13 1.85 -2.27 -0.85 -3.53 -3.99 -2.83 -3.34 -1.39 -3.10 -2.57 1.39 -3.54 -1.53 -4.44
Standard name
Human Ortholog
Description Transcriptional modulator; involved in regulation of meiosis, silencing, and expression of RNR genes; involved in response to replication stress; contains WD repeats; relocalizes to the cytosol in response to hypoxia; WTM2 has a paralog, UME1, that arose from the whole genome duplication
Localization
Cell Percentages nucleus (99%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Wtm2

Wtm2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Wtm2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available