Standard name
Human Ortholog
Description Dihydrofolate reductase involved in tetrahydrofolate biosynthesis; required for respiratory metabolism; mutation is functionally complemented by human DHFR
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.09 0 0 0 0 0 0
Cytoplasm 0.96 0.89 0.94 0.94 0.77 0.67 0.52 – 0.36 0.37 0.22 0.33 0.98 0.97 0.99 0.98 1.0 0.95 0.9 0.77 0.77 0.79 0.75 0.74
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0.07 – 0.06 0.07 0.05 0.05 0 0 0 0 0 0 0 0 0.05 0.07 0.06 0.05
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.22 0.06 – 0.36 0.3 0.5 0.35 0 0 0 0 0 0 0 0 0.05 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0.05 0.05 0.31 0.21 0.44 – 0.34 0.37 0.3 0.25 0 0 0 0 0 0 0 0.09 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 0 0 0 1 2 – 3 2 2 3 0 0 0 0 0 0 0 1 1 0 3 2
Bud 0 0 0 0 1 3 1 – 4 6 6 7 1 0 0 0 0 0 0 0 0 1 2 6
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 4
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 3 4 1 3 2 4 6 – 3 5 3 6 3 2 5 3 0 10 0 0 0 0 0 0
Cytoplasm 326 134 61 140 181 116 158 – 91 89 48 69 183 235 313 90 35 107 313 138 71 74 154 203
Endoplasmic Reticulum 8 5 2 1 0 1 0 – 7 3 2 6 2 2 3 2 1 3 3 0 2 2 5 7
Endosome 3 0 0 1 8 4 20 – 16 17 11 11 0 1 0 0 0 0 11 6 5 6 12 13
Golgi 0 0 0 1 0 0 0 – 1 2 0 2 0 0 0 0 0 0 2 2 1 0 2 3
Mitochondria 1 6 0 3 1 39 17 – 90 71 109 73 0 1 0 1 0 1 1 3 4 1 3 3
Nucleus 1 2 0 0 2 3 6 – 4 4 11 8 0 0 0 0 0 1 1 4 0 0 0 2
Nuclear Periphery 0 1 0 0 0 0 1 – 0 3 0 4 0 0 0 0 0 0 3 1 0 0 1 3
Nucleolus 0 1 0 0 1 0 2 – 0 0 4 0 0 1 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 2 0 0 0 0 0 0 1 0 1 1 3 3
Vac/Vac Membrane 6 1 3 8 72 37 134 – 86 89 64 52 1 3 4 2 0 4 3 15 1 3 9 10
Unique Cell Count 340 151 65 149 234 174 302 251 240 216 208 186 242 317 92 35 113 348 180 94 94 206 274
Labelled Cell Count 349 154 67 157 268 208 347 305 291 260 243 190 245 325 98 36 126 348 180 94 94 206 274


Cytoplasm, Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.0 9.8 9.5 9.1 10.1 6.3 8.8 – 7.4 7.8 6.5 7.6 11.5 9.4 11.1 16.5 16.1 16.9 10.3 11.2 11.5
Std Deviation (1e-4) 1.6 1.9 1.6 1.1 1.4 1.5 1.7 – 1.5 1.1 1.2 1.4 1.4 1.3 1.5 2.9 2.6 3.2 1.8 1.9 2.0
Intensity Change (Log2) – – – -0.05 0.1 -0.59 -0.11 – -0.36 -0.29 -0.53 -0.32 0.29 -0.01 0.23 0.8 0.76 0.84 0.12 0.25 0.28


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 -3.0 -4.3 -6.2 – -8.3 -8.1 -10.4 -8.5 1.9 1.3 2.5 1.3 1.5 0.2
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 4.2 0 – 5.7 5.0 7.3 5.6 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 4.3 3.1 6.0 – 4.7 5.1 4.1 3.6 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.8726 6.0521 5.5894 4.8825 4.3703 5.4218 7.4823 8.9105 8.1584 7.3089 6.9728 8.1176 9.5541 11.986 10.6899 10.0456 9.1305 10.1628
Actin 0.0241 0.0075 0.015 0.0106 0.0101 0.0121 0.0306 0.0135 0.0346 0.0305 0.0278 0.0113 0.0282 0.0061 0.0244 0.0336 0.0497 0.012
Bud 0.0024 0.0014 0.0045 0.008 0.0023 0.0021 0.0031 0.0015 0.0033 0.0052 0.0072 0.0033 0.002 0.0013 0.0018 0.0194 0.0042 0.0011
Bud Neck 0.0026 0.0007 0.0012 0.0027 0.0014 0.0037 0.0023 0.0014 0.0066 0.0019 0.0027 0.0074 0.0015 0.0009 0.0013 0.0014 0.0015 0.0045
Bud Periphery 0.0013 0.0004 0.0016 0.0033 0.0011 0.0013 0.003 0.0005 0.0024 0.004 0.0043 0.0022 0.0012 0.0003 0.0006 0.0161 0.0016 0.0005
Bud Site 0.0059 0.0021 0.0052 0.0036 0.0013 0.001 0.0124 0.0147 0.0117 0.0366 0.0032 0.0009 0.003 0.0056 0.0045 0.0055 0.0022 0.0006
Cell Periphery 0.0007 0.0005 0.0003 0.0004 0.0001 0.0001 0.0009 0.0004 0.0003 0.0002 0.0004 0.0001 0.0003 0.0001 0.0003 0.0004 0.0001 0.0002
Cytoplasm 0.6053 0.7894 0.7734 0.5427 0.6294 0.7014 0.5464 0.802 0.7608 0.7051 0.4869 0.7648 0.6256 0.8726 0.8577 0.7167 0.6246 0.7752
Cytoplasmic Foci 0.0774 0.0611 0.0575 0.1279 0.1189 0.0676 0.0853 0.0536 0.0602 0.0539 0.1143 0.0424 0.0877 0.0473 0.0313 0.0686 0.0747 0.0634
Eisosomes 0.0002 0 0.0001 0.0001 0.0003 0.0001 0.0004 0 0.0001 0.0002 0.0001 0 0.0002 0 0.0001 0 0.0002 0.0001
Endoplasmic Reticulum 0.0192 0.0132 0.0125 0.0351 0.006 0.0097 0.0456 0.0058 0.0064 0.002 0.0057 0.0169 0.0351 0.0037 0.012 0.0048 0.0058 0.0195
Endosome 0.1402 0.0701 0.0673 0.1455 0.1195 0.1197 0.114 0.0506 0.0549 0.0689 0.133 0.0777 0.1138 0.0229 0.0336 0.0533 0.1313 0.0587
Golgi 0.036 0.0156 0.0255 0.05 0.0265 0.0235 0.0238 0.0178 0.0248 0.0229 0.1047 0.015 0.0276 0.0217 0.0157 0.0276 0.0257 0.0143
Lipid Particles 0.0053 0.0002 0.002 0.0125 0.0048 0.0004 0.0082 0.0004 0.0052 0.0006 0.0048 0.0025 0.0028 0.0002 0.0003 0.0001 0.0182 0.0022
Mitochondria 0.0251 0.0171 0.0122 0.0176 0.0414 0.0221 0.0152 0.004 0.0106 0.0119 0.062 0.0295 0.0103 0.0054 0.0059 0.0379 0.0122 0.018
None 0.008 0.0014 0.003 0.0032 0.0089 0.0028 0.0075 0.0019 0.0015 0.0009 0.0014 0.0031 0.0101 0.0044 0.0031 0.003 0.0033 0.0046
Nuclear Periphery 0.0103 0.0065 0.0028 0.0048 0.0033 0.0045 0.0345 0.0022 0.0024 0.0009 0.0108 0.0039 0.0115 0.0006 0.0012 0.0015 0.0025 0.0023
Nucleolus 0.0026 0 0.0001 0.0003 0.0002 0.0002 0.0006 0.0001 0.0001 0.0001 0.0003 0.0002 0.0002 0 0 0 0.0001 0.0001
Nucleus 0.0066 0.0029 0.0023 0.0039 0.0033 0.0058 0.0139 0.0126 0.002 0.0455 0.0031 0.0059 0.0149 0.0017 0.0017 0.0028 0.002 0.0034
Peroxisomes 0.0105 0.0049 0.0071 0.0123 0.0099 0.0086 0.0191 0.0109 0.0061 0.0035 0.0143 0.0027 0.0132 0.0037 0.0021 0.0046 0.0267 0.0138
Punctate Nuclear 0.0027 0.0004 0.0006 0.0022 0.0024 0.0011 0.0053 0.0006 0.0008 0.0008 0.0008 0.0034 0.0018 0.0003 0.0003 0.0004 0.005 0.0018
Vacuole 0.0096 0.0032 0.004 0.0084 0.005 0.0076 0.0214 0.0044 0.0036 0.0029 0.0053 0.0036 0.0063 0.0008 0.0013 0.0013 0.0045 0.0021
Vacuole Periphery 0.004 0.0015 0.0017 0.0049 0.0038 0.0046 0.0064 0.0011 0.0017 0.0014 0.0069 0.0031 0.0027 0.0003 0.0008 0.001 0.0038 0.0015

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 26.0237 23.2636 25.3185 28.3677 39.3643 31.2111 31.3624 31.7512 29.0043 42.7978
Translational Efficiency 1.7168 1.6253 1.2052 0.9913 0.9472 1.2084 1.194 1.0016 1.076 1.078

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
453 878 637 2423 2597 1815 849 1820 3050 2693 1486 4243

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 909.84 1098.93 1285.15 1096.83 1015.75 1127.07 1101.10 1119.18 1000.02 1117.90 1180.00 1106.42
Standard Deviation 139.28 176.36 149.48 154.29 131.41 154.14 139.98 165.68 137.85 162.26 170.50 159.66
Intensity Change Log 2 — 0.272415 0.498252 0.269655 — 0.150032 0.116400 0.139897 — 0.209153 0.309445 0.202664

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.004176 0.001548 0.000399 0.017366 0.000616 0.001904 0.002253 0.002056 0.001145 0.001788 0.001458 0.010799
Bud Neck 0.008729 0.005502 0.002241 0.016499 0.007827 0.011333 0.010007 0.012127 0.007961 0.009432 0.006678 0.014623
Bud Site 0.181237* 0.095101 0.003184 0.144528* 0.029228 0.081719 0.095314 0.055072 0.051805 0.086082 0.055821 0.106157*
Cell Periphery 0.001742 0.000132 0.000120 0.000134 0.000076 0.000092 0.000095 0.000103 0.000323 0.000105 0.000106 0.000121
Cytoplasm 0.074669 0.529147* 0.544017* 0.491844* 0.602321* 0.507767* 0.544660* 0.424047* 0.523952* 0.514737* 0.544384* 0.462763*
Cytoplasmic Foci 0.206618* 0.187261* 0.008132 0.105713 0.203563* 0.224555* 0.119677 0.110583 0.204017* 0.212396* 0.071861 0.107802
Eisosomes 0.000150 0.000019 0.000011 0.000059 0.000023 0.000032 0.000019 0.000023 0.000042 0.000028 0.000016 0.000044
Endoplasmic Reticulum 0.006082 0.001315 0.001577 0.000832 0.002070 0.001094 0.000918 0.002203 0.002666 0.001166 0.001201 0.001420
Endosome 0.066835 0.026887 0.001317 0.015471 0.016164 0.023781 0.024686 0.032058 0.023690 0.024793 0.014668 0.022586
Golgi 0.137574* 0.033494 0.000184 0.021957 0.012445 0.028996 0.014047 0.007618 0.031030 0.030463 0.008104 0.015807
Lipid Particles 0.001386 0.001099 0.000761 0.001217 0.001813 0.001003 0.001221 0.001846 0.001749 0.001034 0.001023 0.001486
Mitochondria 0.192318* 0.006204 0.000333 0.013326 0.006812 0.010103 0.003533 0.007199 0.034365 0.008831 0.002161 0.010698
Mitotic Spindle 0.017141 0.019074 0.002674 0.025667 0.008082 0.013516 0.034358 0.045813 0.009427 0.015328 0.020776 0.034308
None 0.000335 0.000887 0.006761 0.000840 0.001266 0.000800 0.000718 0.001074 0.001128 0.000828 0.003308 0.000940
Nuclear Periphery 0.000073 0.000136 0.000872 0.000098 0.000239 0.000078 0.000216 0.000815 0.000215 0.000097 0.000497 0.000406
Nuclear Periphery Foci 0.000316 0.001645 0.003347 0.000382 0.000766 0.000567 0.001514 0.001707 0.000699 0.000919 0.002300 0.000950
Nucleolus 0.000129 0.000458 0.000161 0.000279 0.000511 0.000332 0.000542 0.001087 0.000454 0.000373 0.000379 0.000626
Nucleus 0.001556 0.012981 0.370357* 0.014315 0.036997 0.014182 0.023206 0.028040 0.031733 0.013790 0.172018* 0.020202
Peroxisomes 0.011692 0.008075 0.000081 0.047352 0.004312 0.011129 0.015006 0.012247 0.005408 0.010134 0.008608 0.032294
Vacuole 0.034389 0.056516 0.053332 0.078359 0.059414 0.059596 0.102197 0.243921* 0.055697 0.058591 0.081251 0.149375
Vacuole Periphery 0.052854 0.012521 0.000141 0.003763 0.005453 0.007424 0.005812 0.010359 0.012494 0.009086 0.003381 0.006592

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 3.13 4.42 -7.36 -11.32 -12.36 -3.85 -4.95 -3.01 0.39 0.82 -2.38 -1.26 -11.46 -10.31 -11.09
Bud Neck 2.84 5.28 -5.20 -10.53 -13.73 -3.62 -1.97 -4.61 -0.25 -1.89 -2.02 1.88 -8.49 -5.34 -9.53
Bud Site 7.66 16.97 5.47 -4.81 -35.34* -15.78 -9.93 -6.14 9.64 6.21 -9.78 1.59 -11.98 -1.28 -12.11
Cell Periphery 4.09 4.00 4.11 0.55 3.87 -2.02 -1.94 -1.04 0.26 0.31 3.66 3.34 3.58 -0.62 1.97
Cytoplasm -34.75* -36.50 -48.22 2.57 -1.25 9.69 4.21 17.93 7.01 9.01 0.80 0.11 6.75 5.74 5.92
Cytoplasmic Foci 2.11 23.58 12.74 12.47 -31.23* -3.08 15.59 20.56 21.83 1.42 -1.25 28.94 25.15 25.72 -8.88
Eisosomes 10.46 10.57 7.59 -13.51 -14.44 -4.48 1.92 -0.13 4.59 -2.20 5.65 9.67 0.43 -7.28 -13.81
Endoplasmic Reticulum 3.38 2.86 3.74 1.87 6.63 3.12 4.46 0.81 -2.16 -3.25 4.52 4.06 4.41 -0.46 1.09
Endosome 8.92 15.88 13.00 6.53 -16.24 -4.92 -2.03 -5.35 -0.37 -2.20 -0.55 8.53 4.95 5.38 -4.99
Golgi 12.58 17.32 15.09 5.19 -13.79 -9.27 1.43 7.97 13.73 3.06 0.62 14.26 11.27 11.37 -5.29
Lipid Particles 0.96 0.53 -0.54 -3.06 -2.34 5.06 2.05 -1.14 -7.47 -3.68 5.16 2.75 0.09 -7.13 -3.71
Mitochondria 15.51 16.03 15.15 -3.17 -10.21 -2.84 5.29 1.76 4.02 -2.58 11.09 14.69 11.42 0.56 -9.07
Mitotic Spindle -0.53 5.02 -2.93 -2.52 -11.30 -2.90 -7.05 -10.35 -8.13 -1.80 -3.65 -5.05 -12.01 -8.21 -4.69
None -4.03 -9.57 -1.19 0.24 7.17 3.27 3.70 1.01 -0.92 -0.83 2.66 -6.76 0.96 -0.26 5.74
Nuclear Periphery -1.63 -19.84 -5.84 -0.90 18.02 2.46 -1.01 -5.98 -8.83 -5.93 2.02 -7.93 -4.16 -8.98 4.20
Nuclear Periphery Foci -5.78 -11.24 -2.89 4.95 10.69 1.50 -5.35 -3.80 -4.65 0.82 -2.17 -10.86 -2.67 -0.62 8.75
Nucleolus -3.07 -1.70 -5.61 1.07 -6.10 2.70 -0.97 -5.84 -7.91 -4.15 1.39 0.43 -3.60 -5.09 -3.89
Nucleus -9.34 -40.61 -19.26 -5.91 37.94 15.06 1.60 0.04 -13.30 -1.49 13.43 -24.14 1.68 -13.51 24.69
Peroxisomes 2.47 9.18 -12.22 -15.27 -21.08* -8.25 -5.28 -6.27 1.01 1.30 -6.87 -1.70 -17.80 -13.02 -13.98
Vacuole -5.59 -9.91 -18.30 -11.69 -2.11 -0.20 -12.59 -33.98 -32.80 -16.62 -1.74 -14.40 -34.00 -31.26 -11.55
Vacuole Periphery 8.62 11.61 10.98 6.43 -9.03 -2.61 1.55 -2.10 0.33 -2.98 3.56 11.37 8.92 5.85 -4.44
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Dihydrofolate reductase involved in tetrahydrofolate biosynthesis; required for respiratory metabolism; mutation is functionally complemented by human DHFR
Localization
Cell Percentages cytoplasm (82%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-5

Dfr1

Dfr1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Dfr1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available