Standard name
Human Ortholog
Description Pseudouridine synthase; catalyzes pseudouridylation at positions 35 and 56 in U2 snRNA, position 50 in 5S rRNA, position 13 in cytoplasmic tRNAs, and position 35 in pre-tRNA(Tyr); also pseudouridylates some mRNAs; relocates from nucleus to cytoplasm during heat shock and differentially modifies some mRNAs during heat shock; conserved in archaea, vertebrates, and some bacteria
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Cytoplasm 0 0 0.05 0.05 0.09 0.12 0.05 0.06 0.11 0.05 0 0 0.07 0.13 0.1 – – – 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.05 0 0.16 0.06 0 0 0 – – – 0 0 0 0 0 0
Nucleus 0.95 0.96 0.95 0.95 0.9 0.83 0.89 0.88 0.78 0.86 0.81 0.85 0.91 0.84 0.82 – – – 0.93 0.93 0.93 0.89 0.76 0.67
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0 0 0.05 0.05 0 0 0.07 0 0.13 0.13 0.25 0.18 0 0 0.08 – – – 0 0 0 0 0 0.06
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0.06 0.16 0.2
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 – – – 2 2 0 1 1 0
Bud Neck 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 1 1 2 1 3 8 0 0 0 – – – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Cytoplasm 5 6 2 2 15 23 12 17 15 12 8 7 11 25 17 – – – 0 0 0 1 0 3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 – – – 0 1 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 1 0 0 2 1 0 – – – 0 0 0 0 1 1
Golgi 0 0 0 0 1 1 0 0 1 1 0 0 0 1 0 – – – 0 1 0 0 0 1
Mitochondria 2 2 1 1 0 3 6 8 7 6 33 17 0 0 0 – – – 1 1 0 0 2 1
Nucleus 191 243 39 39 158 155 226 242 106 220 169 231 140 163 138 – – – 189 242 64 96 145 143
Nuclear Periphery 0 0 0 0 0 0 5 2 2 0 1 0 1 0 2 – – – 0 0 0 0 0 1
Nucleolus 2 0 2 2 1 5 18 12 18 34 53 49 2 1 13 – – – 0 0 0 0 5 12
Peroxisomes 1 0 0 0 0 0 0 0 0 0 0 1 0 2 3 – – – 0 0 0 0 0 0
SpindlePole 1 0 0 0 0 0 1 1 0 1 0 1 0 2 3 – – – 0 0 0 0 0 0
Vac/Vac Membrane 2 2 0 0 3 1 0 1 1 3 0 6 4 0 1 – – – 8 7 0 6 29 42
Unique Cell Count 200 253 41 41 175 186 254 276 136 256 209 271 154 194 169 205 260 69 109 191 213
Labelled Cell Count 204 253 44 44 178 188 269 284 153 280 268 321 160 196 177 205 260 69 109 191 213


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.6 11.2 8.5 8.5 10.4 8.5 8.0 8.3 7.6 7.9 6.6 7.1 13.3 11.6 10.9 – – – 9.7 11.1 11.0
Std Deviation (1e-4) 1.8 2.4 1.7 1.7 2.2 2.3 1.9 2.0 1.9 2.0 1.7 1.7 13.6 3.4 2.8 – – – 2.2 2.5 2.2
Intensity Change (Log2) – – – 0.0 0.28 -0.0 -0.08 -0.04 -0.17 -0.1 -0.36 -0.26 0.64 0.45 0.36 – – – 0.2 0.39 0.37


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud Neck – 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud Site – 0 0 0 0 0 0 0 0 0 0 0 – – –
Cell Periphery – 0 0 0 0 0 0 0 0 0 0 0 – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – 0 0 0 0 0 0 0 0 0 0 0 – – –
Endosome – 0 0 0 0 0 0 0 0 0 0 0 – – –
Golgi – 0 0 0 0 0 0 0 0 0 0 0 – – –
Mitochondria – 0 0 0 0 0 0 2.3 0 0 0 0 – – –
Nuclear Periphery – 0 0 0 0 0 0 0 0 0 0 0 – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – 0 0 0 0 0 0 2.9 2.1 0 0 0 – – –
Peroxisomes – 0 0 0 0 0 0 0 0 0 0 0 – – –
SpindlePole – 0 0 0 0 0 0 0 0 0 0 0 – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – 0 0 0 0 0 0 0 0 0 0 0 – – –
Cytoplasm – 0 0 0 0 0 0 0 0 0 0 0 – – –
Nucleus – -1.0 -1.9 -1.2 -1.4 -2.5 -1.6 -2.2 -1.7 -0.9 -1.9 -2.1 – – –
Vacuole – 0 0 0 0 0 0 0 0 0 0 0 – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 8.4855 10.1061 8.8058 8.4679 8.5563 8.0816 9.3725 9.2793 8.5831 8.8132 7.9535 8.2395 11.4294 10.617 10.1324 9.8483 10.1115 9.8775
Actin 0.0069 0 0.0015 0.0011 0 0.0013 0.0044 0 0.0038 0 0 0 0.0001 0 0 0 0 0.0082
Bud 0.0003 0 0.0001 0 0.0001 0.0002 0.0001 0 0 0 0 0 0 0 0 0.0003 0 0
Bud Neck 0.0003 0 0 0 0.0002 0.001 0.0001 0.0001 0.0001 0 0.0001 0.0003 0 0 0 0.0003 0 0.0002
Bud Periphery 0.0005 0 0.0001 0.0001 0.0001 0.0003 0.0003 0 0 0 0.0001 0 0 0 0 0.0006 0 0.0001
Bud Site 0.0009 0 0.0002 0.0001 0.0001 0.0002 0.0004 0 0.0001 0 0 0 0.0002 0 0 0.0004 0 0
Cell Periphery 0.0001 0 0 0 0.0002 0.0002 0.0001 0 0.0001 0 0 0 0.0001 0 0 0.0004 0 0
Cytoplasm 0.0098 0 0.0012 0 0.0001 0.0008 0.001 0 0.0004 0 0 0 0.0002 0 0 0.0002 0 0
Cytoplasmic Foci 0.0084 0 0.0042 0.0039 0 0.0016 0.004 0 0.0008 0 0 0 0.0008 0 0 0 0 0.0004
Eisosomes 0.0001 0 0 0 0 0 0.0001 0 0.0002 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0022 0 0.0007 0.0001 0.0001 0.001 0.0003 0 0.0002 0 0 0 0.0001 0 0 0.0001 0 0.0001
Endosome 0.0131 0 0.0024 0.0079 0.0002 0.0144 0.0027 0 0.0006 0 0 0 0.0002 0 0 0.0001 0 0.0009
Golgi 0.0018 0 0.0004 0.0098 0 0.0041 0.0009 0 0.0005 0 0 0 0.0001 0 0 0 0 0.0009
Lipid Particles 0.0038 0 0.0018 0.0229 0.0001 0.004 0.0023 0 0.0019 0 0 0 0.0009 0 0 0 0 0.0012
Mitochondria 0.0061 0.0003 0.0011 0.0048 0.0008 0.0014 0.0015 0.0001 0.0005 0.0001 0.0004 0.0001 0.0007 0.0002 0.0009 0.0007 0.0001 0.0004
None 0.0024 0 0.0007 0 0 0.0004 0.004 0 0.0004 0 0 0 0.0006 0 0 0 0 0.0001
Nuclear Periphery 0.0218 0.0021 0.0027 0.0006 0.0031 0.0141 0.0018 0.0005 0.0018 0.001 0.0013 0.0002 0.0004 0.0005 0.001 0.0003 0.0002 0.0007
Nucleolus 0.0339 0.0069 0.0072 0.0043 0.1181 0.0731 0.0325 0.022 0.0065 0.0043 0.1648 0.0393 0.0103 0.0098 0.006 0.0164 0.0725 0.0267
Nucleus 0.8663 0.9904 0.9657 0.9398 0.8698 0.8761 0.9307 0.9769 0.9791 0.9945 0.8306 0.9599 0.984 0.9891 0.9913 0.9787 0.9268 0.9587
Peroxisomes 0.0024 0 0.0037 0.0035 0 0.0004 0.0097 0 0.0012 0 0 0 0.0001 0 0 0 0 0.0005
Punctate Nuclear 0.0144 0.0002 0.0044 0.0004 0.0003 0.0029 0.0024 0.0001 0.0009 0 0.0022 0.0001 0.0007 0 0.0001 0.0001 0.0002 0.0006
Vacuole 0.0025 0 0.001 0.0002 0.0034 0.0012 0.0003 0.0001 0.0005 0 0.0001 0.0001 0.0002 0.0001 0.0001 0.0011 0 0.0001
Vacuole Periphery 0.0021 0.0001 0.001 0.0003 0.0032 0.001 0.0003 0.0001 0.0005 0.0001 0.0002 0 0.0003 0.0002 0.0003 0.0002 0 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 76.6027 77.22 59.8232 58.156 53.8857 43.9814 54.2611 51.5368 43.6829 61.2042
Translational Efficiency 1.3669 1.2721 1.3758 1.2 1.4322 1.679 1.2125 0.8635 1.0034 1.0776

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1693 1217 570 1365 2080 1687 1754 191 3773 2904 2324 1556

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 718.88 1073.08 1286.98 1443.40 997.00 1082.79 1228.62 1519.91 872.20 1078.72 1242.93 1452.79
Standard Deviation 121.26 254.19 183.31 247.32 153.30 186.20 184.75 274.77 196.69 217.35 186.10 252.10
Intensity Change Log 2 — 0.577935 0.840167 1.005648 — 0.119088 0.301373 0.608320 — 0.329322 0.551954 0.788261

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000298 0.000590 0.000556 0.000594 0.000216 0.000394 0.000467 0.000707 0.000252 0.000476 0.000488 0.000608
Bud Neck 0.001811 0.005131 0.011040 0.015607 0.002793 0.006023 0.008644 0.017123 0.002352 0.005649 0.009232 0.015793
Bud Site 0.001098 0.001070 0.000603 0.001443 0.000212 0.001207 0.000538 0.001601 0.000610 0.001150 0.000554 0.001462
Cell Periphery 0.000848 0.000411 0.000060 0.000176 0.000238 0.000473 0.000111 0.000065 0.000512 0.000447 0.000098 0.000162
Cytoplasm 0.010140 0.001796 0.000077 0.000517 0.000069 0.000276 0.000125 0.000599 0.004588 0.000913 0.000113 0.000527
Cytoplasmic Foci 0.000205 0.000860 0.000010 0.000518 0.000013 0.000115 0.000011 0.000491 0.000099 0.000427 0.000011 0.000515
Eisosomes 0.000040 0.000078 0.000058 0.000044 0.000099 0.000151 0.000074 0.000034 0.000073 0.000120 0.000070 0.000043
Endoplasmic Reticulum 0.007705 0.004673 0.001873 0.001255 0.005839 0.005881 0.002517 0.001214 0.006677 0.005375 0.002359 0.001250
Endosome 0.000267 0.000812 0.000031 0.000522 0.000093 0.000707 0.000136 0.000162 0.000171 0.000751 0.000111 0.000478
Golgi 0.000056 0.000350 0.000028 0.001031 0.000018 0.000042 0.000022 0.000069 0.000035 0.000171 0.000024 0.000913
Lipid Particles 0.000053 0.000262 0.000018 0.000056 0.000017 0.000495 0.000052 0.000046 0.000033 0.000397 0.000044 0.000055
Mitochondria 0.000383 0.000971 0.000932 0.002027 0.000348 0.000617 0.001109 0.001714 0.000364 0.000766 0.001065 0.001988
Mitotic Spindle 0.000102 0.004815 0.000018 0.004917 0.000193 0.000155 0.000098 0.007067 0.000152 0.002108 0.000078 0.005181
None 0.030930 0.005264 0.000561 0.001396 0.000729 0.000813 0.000571 0.001178 0.014281 0.002678 0.000568 0.001369
Nuclear Periphery 0.000111 0.000296 0.000084 0.000715 0.000085 0.000173 0.000245 0.000237 0.000097 0.000224 0.000205 0.000656
Nuclear Periphery Foci 0.000431 0.000570 0.000007 0.000363 0.000013 0.000080 0.000112 0.000068 0.000201 0.000285 0.000086 0.000326
Nucleolus 0.007386 0.007133 0.005459 0.006138 0.004996 0.005141 0.003599 0.007464 0.006068 0.005976 0.004055 0.006301
Nucleus 0.936536* 0.961371* 0.977689* 0.959971* 0.982907* 0.974340* 0.980748* 0.958677* 0.962100* 0.968905* 0.979998* 0.959812*
Peroxisomes 0.000113 0.000433 0.000403 0.000225 0.000174 0.000384 0.000162 0.000472 0.000147 0.000405 0.000221 0.000256
Vacuole 0.001390 0.002137 0.000429 0.001414 0.000791 0.002144 0.000472 0.000714 0.001060 0.002141 0.000461 0.001328
Vacuole Periphery 0.000096 0.000976 0.000064 0.001071 0.000155 0.000390 0.000187 0.000300 0.000128 0.000635 0.000157 0.000976

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.17 -9.68 -9.38 -0.39 -1.81 -4.65 -5.85 -3.46 -2.61 -2.22 -4.91 -7.22 -10.50 -3.34 -3.64
Bud Neck -3.96 -9.52 -13.39 -8.92 -4.16 -4.16 -9.49 -3.93 -3.25 -2.62 -5.76 -12.99 -13.36 -9.69 -6.57
Bud Site -0.09 2.00 -1.29 -1.04 -3.64 -2.93 -4.85 -2.38 -1.11 -1.95 -2.22 0.25 -3.88 -1.32 -4.26
Cell Periphery 3.03 6.42 4.64 2.17 -1.13 -2.00 1.76 2.13 4.04 0.88 0.71 6.02 3.59 3.01 -0.69
Cytoplasm 5.11 6.95 6.59 2.14 -5.16 -1.61 -1.50 -2.82 -1.52 -2.52 5.01 6.83 6.06 1.39 -5.23
Cytoplasmic Foci -1.31 3.88 -0.94 0.80 -2.07 -1.89 0.15 -1.03 -0.84 -1.04 -1.56 3.79 -1.75 -0.13 -2.30
Eisosomes -3.42 -3.14 -0.61 3.41 3.85 -1.94 2.63 6.19 4.48 6.61 -2.96 0.56 5.12 5.11 7.21
Endoplasmic Reticulum 5.35 12.86 14.39 9.31 5.06 -0.09 11.89 6.10 5.67 1.18 3.63 16.49 20.60 14.25 6.99
Endosome -2.13 4.56 -1.23 1.18 -2.79 -1.37 -0.46 -0.81 1.06 -0.43 -2.07 0.83 -1.89 1.01 -2.13
Golgi -2.42 3.70 -1.69 -1.01 -1.75 -1.29 -0.49 -2.62 -1.81 -2.54 -2.61 1.42 -1.76 -1.41 -1.78
Lipid Particles -2.42 4.55 -0.32 2.19 -1.52 -1.12 -1.02 -1.66 0.97 -0.53 -1.47 -0.37 -1.17 1.33 -0.65
Mitochondria -4.51 -11.47 -13.60 -6.85 -8.97 -2.50 -2.66 -6.70 -5.82 -2.54 -4.91 -3.32 -14.15 -10.54 -4.55
Mitotic Spindle -2.86 1.74 -3.69 -0.19 -3.75 0.11 -0.61 -1.69 -1.70 -1.66 -2.81 -0.63 -3.97 -2.40 -3.83
None 8.86 11.57 11.06 2.82 -1.83 -0.33 0.59 -1.74 -1.86 -2.76 8.82 11.32 10.04 1.80 -2.03
Nuclear Periphery -1.64 1.41 -4.77 -2.96 -4.88 -2.68 -1.78 -3.20 -2.13 -0.92 -2.54 -1.65 -5.09 -3.93 -3.74
Nuclear Periphery Foci -0.58 3.61 0.47 0.93 -3.55 -1.55 -1.27 -1.79 -0.34 0.02 -0.82 1.57 -1.25 -0.35 -2.40
Nucleolus 0.22 2.45 -1.18 -1.10 -3.01 -0.29 1.89 -2.40 -2.32 -2.72 0.11 3.81 -3.32 -3.07 -5.19
Nucleus -4.62 -10.49 -3.65 1.27 6.41 3.85 2.40 3.85 3.03 3.51 -2.47 -8.22 2.59 4.25 7.78
Peroxisomes -3.06 -8.23 -5.38 1.80 3.96 -0.88 0.13 -1.79 -1.07 -1.86 -1.81 -1.68 -2.76 0.58 -2.03
Vacuole -1.36 4.64 -0.03 1.14 -2.45 -3.35 2.66 -1.21 2.16 -2.37 -3.28 5.09 -0.88 1.64 -2.60
Vacuole Periphery -2.27 4.07 -2.53 0.04 -2.61 -2.53 -0.25 -0.93 0.27 -0.67 -2.97 -0.31 -2.50 -0.71 -2.31
Standard name
Human Ortholog
Description Pseudouridine synthase; catalyzes pseudouridylation at positions 35 and 56 in U2 snRNA, position 50 in 5S rRNA, position 13 in cytoplasmic tRNAs, and position 35 in pre-tRNA(Tyr); also pseudouridylates some mRNAs; relocates from nucleus to cytoplasm during heat shock and differentially modifies some mRNAs during heat shock; conserved in archaea, vertebrates, and some bacteria
Localization
Cell Percentages nucleus (100%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Pus7

Pus7


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pus7-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available