Standard name | |
---|---|
Human Ortholog | |
Description | Diacylglycerol acyltransferase; catalyzes the terminal step of triacylglycerol (TAG) formation, acylates diacylglycerol using acyl-CoA as an acyl donor; Lro1p and Dga1p can O-acylate ceramides; localized to lipid particles |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 2 | 1 | 5 | 5 | 1 | 1 | 1 | 0 | 1 | 2 | 1 | 1 | 2 | 2 | 6 | 13 | 11 | 11 | 5 | 2 | 11 | 3 | 13 | 8 |
Bud | 2 | 0 | 4 | 9 | 8 | 7 | 37 | 16 | 19 | 22 | 34 | 22 | 0 | 0 | 0 | 1 | 1 | 2 | 3 | 0 | 0 | 0 | 5 | 9 |
Bud Neck | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 2 | 5 | 4 | 4 | 7 | 10 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 0 | 3 | 6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 11 | 5 | 8 | 17 | 11 | 13 | 12 | 9 | 10 | 10 | 4 | 7 | 4 | 31 | 14 | 22 | 21 | 14 | 2 | 0 | 6 | 4 | 15 | 16 |
Cytoplasm | 85 | 58 | 140 | 208 | 329 | 259 | 366 | 405 | 237 | 240 | 187 | 205 | 179 | 288 | 272 | 131 | 111 | 95 | 31 | 43 | 24 | 94 | 263 | 280 |
Endoplasmic Reticulum | 33 | 40 | 78 | 32 | 32 | 32 | 23 | 34 | 29 | 69 | 15 | 26 | 49 | 103 | 129 | 5 | 4 | 5 | 141 | 67 | 235 | 41 | 90 | 116 |
Endosome | 1 | 0 | 5 | 7 | 18 | 4 | 23 | 22 | 5 | 5 | 6 | 6 | 2 | 2 | 1 | 19 | 2 | 9 | 1 | 0 | 3 | 2 | 5 | 7 |
Golgi | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 3 | 0 | 0 | 0 | 3 | 1 | 0 | 0 |
Mitochondria | 21 | 5 | 10 | 63 | 56 | 134 | 360 | 296 | 298 | 324 | 292 | 312 | 0 | 1 | 0 | 12 | 8 | 10 | 25 | 5 | 37 | 21 | 55 | 36 |
Nucleus | 1 | 0 | 0 | 0 | 0 | 2 | 1 | 9 | 7 | 7 | 6 | 7 | 0 | 1 | 1 | 5 | 1 | 4 | 0 | 0 | 0 | 0 | 1 | 5 |
Nuclear Periphery | 2 | 1 | 0 | 0 | 0 | 1 | 5 | 7 | 1 | 6 | 4 | 11 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 1 |
Nucleolus | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 3 | 2 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 1 | 0 | 0 | 2 | 2 | 3 | 1 | 0 | 0 | 0 | 1 | 0 | 4 | 2 | 0 | 0 | 1 | 0 | 0 | 1 | 2 | 2 |
Vac/Vac Membrane | 5 | 0 | 7 | 12 | 29 | 23 | 87 | 127 | 78 | 73 | 70 | 87 | 4 | 10 | 1 | 55 | 29 | 37 | 10 | 6 | 4 | 8 | 20 | 35 |
Unique Cell Count | 144 | 101 | 241 | 305 | 439 | 396 | 710 | 776 | 559 | 604 | 479 | 531 | 231 | 396 | 392 | 239 | 167 | 165 | 234 | 134 | 333 | 188 | 490 | 540 |
Labelled Cell Count | 164 | 110 | 260 | 357 | 486 | 479 | 918 | 934 | 689 | 763 | 627 | 687 | 241 | 440 | 428 | 267 | 192 | 187 | 234 | 134 | 333 | 188 | 490 | 540 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.8 | 5.5 | 5.3 | 5.2 | 5.2 | 4.5 | 4.4 | 4.6 | 4.3 | 4.3 | 4.2 | 4.4 | 6.4 | 6.6 | 6.5 | 8.2 | 8.4 | 7.3 | 6.4 | 6.4 | 6.8 |
Std Deviation (1e-4) | 0.6 | 0.9 | 1.0 | 1.7 | 1.6 | 1.2 | 1.3 | 1.3 | 1.0 | 0.7 | 1.0 | 0.8 | 0.7 | 0.8 | 0.8 | 1.4 | 1.5 | 1.4 | 1.3 | 1.1 | 1.6 |
Intensity Change (Log2) | – | – | – | -0.03 | -0.02 | -0.22 | -0.28 | -0.2 | -0.31 | -0.29 | -0.35 | -0.27 | 0.28 | 0.32 | 0.29 | 0.62 | 0.65 | 0.47 | 0.27 | 0.27 | 0.36 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.9 | 2.3 | 2.3 |
Bud | 1.0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 1.3 | -0.6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -1.1 | 2.3 | 0.2 | 2.7 | 3.6 | 2.3 |
Cytoplasm | 2.4 | 4.5 | 1.9 | -1.8 | -1.6 | -4.1 | -4.8 | -4.8 | -5.0 | 4.5 | 3.8 | 2.9 | -0.7 | 1.7 | -0.1 |
Endoplasmic Reticulum | -6.3 | -8.5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -2.7 | -1.7 | 0.1 | -8.8 | -7.4 | -7.2 |
Endosome | 0.2 | 1.4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.0 | 0 | 1.8 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 5.6 | 3.6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.5 | 0.3 | 0.9 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0.7 | 2.1 | 1.7 | 4.2 | 5.4 | 4.7 | 4.1 | 4.8 | 5.3 | -0.8 | -0.3 | 0 | 6.6 | 5.1 | 6.2 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.5583 | 3.5322 | 3.5665 | 2.5799 | 2.957 | 3.341 | -0.5332 | 0.7011 | 0.138 | -0.2034 | -0.133 | 0.2943 | 0.6898 | 0.8763 | 0.5607 | 0.701 | 0.5462 | 0.4262 |
Actin | 0.0005 | 0.0025 | 0.0046 | 0.0043 | 0.0181 | 0.0025 | 0.0139 | 0.0046 | 0.0093 | 0.0067 | 0.0044 | 0.0018 | 0.0212 | 0.0013 | 0.0143 | 0.0153 | 0.0325 | 0.0035 |
Bud | 0.0003 | 0.0012 | 0.0004 | 0.0028 | 0.0003 | 0.0004 | 0.0002 | 0.0002 | 0.0001 | 0.0031 | 0.0006 | 0.0001 | 0.0034 | 0.0182 | 0.0028 | 0.0025 | 0.0063 | 0.0013 |
Bud Neck | 0.0011 | 0.0012 | 0.0009 | 0.0018 | 0.0018 | 0.0043 | 0.0006 | 0.001 | 0.0005 | 0.0006 | 0.0016 | 0.0033 | 0.0047 | 0.0004 | 0.0015 | 0.0018 | 0.0018 | 0.0032 |
Bud Periphery | 0.0005 | 0.0016 | 0.0005 | 0.0051 | 0.0006 | 0.0008 | 0.0003 | 0.0003 | 0.0001 | 0.0015 | 0.0005 | 0.0003 | 0.0017 | 0.0026 | 0.0012 | 0.0078 | 0.002 | 0.0021 |
Bud Site | 0.004 | 0.0114 | 0.0057 | 0.0021 | 0.0005 | 0.0002 | 0.0016 | 0.0072 | 0.0011 | 0.0175 | 0.0009 | 0.0002 | 0.008 | 0.0091 | 0.003 | 0.0041 | 0.0023 | 0.0006 |
Cell Periphery | 0.0026 | 0.0025 | 0.0028 | 0.0022 | 0.0009 | 0.002 | 0.0014 | 0.0027 | 0.001 | 0.0006 | 0.0006 | 0.0004 | 0.0012 | 0.0017 | 0.0013 | 0.0013 | 0.0005 | 0.0007 |
Cytoplasm | 0.2911 | 0.402 | 0.4091 | 0.5186 | 0.4031 | 0.4777 | 0.3641 | 0.4385 | 0.4171 | 0.4047 | 0.5005 | 0.5514 | 0.3088 | 0.4637 | 0.4048 | 0.4897 | 0.3829 | 0.4815 |
Cytoplasmic Foci | 0.0174 | 0.0353 | 0.0304 | 0.0269 | 0.0508 | 0.0273 | 0.0393 | 0.0388 | 0.031 | 0.0532 | 0.0272 | 0.0317 | 0.0495 | 0.0222 | 0.063 | 0.0302 | 0.0512 | 0.0274 |
Eisosomes | 0.0001 | 0.0003 | 0.0003 | 0.0002 | 0.0003 | 0.0001 | 0.0003 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0002 | 0.0003 | 0.0002 | 0.0001 |
Endoplasmic Reticulum | 0.26 | 0.1228 | 0.2769 | 0.1668 | 0.1648 | 0.2405 | 0.2852 | 0.3009 | 0.3059 | 0.2567 | 0.0846 | 0.2426 | 0.2886 | 0.278 | 0.2336 | 0.2399 | 0.2031 | 0.2805 |
Endosome | 0.0351 | 0.0891 | 0.0391 | 0.0151 | 0.037 | 0.0327 | 0.066 | 0.0428 | 0.0727 | 0.0594 | 0.1732 | 0.0565 | 0.0747 | 0.0428 | 0.0792 | 0.0386 | 0.0809 | 0.0309 |
Golgi | 0.0018 | 0.0125 | 0.004 | 0.0022 | 0.0072 | 0.0042 | 0.0151 | 0.0032 | 0.0138 | 0.0409 | 0.0178 | 0.0096 | 0.0084 | 0.0022 | 0.0092 | 0.0059 | 0.0266 | 0.0033 |
Lipid Particles | 0.0623 | 0.0121 | 0.0326 | 0.0052 | 0.05 | 0.0334 | 0.0525 | 0.0482 | 0.0207 | 0.0355 | 0.0415 | 0.0106 | 0.0504 | 0.0251 | 0.0297 | 0.0226 | 0.0338 | 0.0302 |
Mitochondria | 0.0005 | 0.0013 | 0.0014 | 0.0016 | 0.0017 | 0.0023 | 0.0043 | 0.0004 | 0.0025 | 0.0175 | 0.0151 | 0.0279 | 0.0038 | 0.002 | 0.0046 | 0.0173 | 0.0086 | 0.0021 |
None | 0.2857 | 0.2809 | 0.1654 | 0.1803 | 0.241 | 0.148 | 0.1154 | 0.0771 | 0.0954 | 0.0414 | 0.0907 | 0.0275 | 0.1152 | 0.0966 | 0.1098 | 0.0665 | 0.122 | 0.0963 |
Nuclear Periphery | 0.0083 | 0.0058 | 0.0087 | 0.0134 | 0.0053 | 0.0098 | 0.0164 | 0.0129 | 0.0148 | 0.0113 | 0.0113 | 0.0182 | 0.0182 | 0.009 | 0.0179 | 0.0115 | 0.0117 | 0.0141 |
Nucleolus | 0.0084 | 0.0007 | 0.0003 | 0.0038 | 0.0004 | 0.0002 | 0.0003 | 0.0007 | 0.0001 | 0.0001 | 0.001 | 0.0001 | 0.0048 | 0.0006 | 0.0012 | 0.0169 | 0.0005 | 0.0005 |
Nucleus | 0.0044 | 0.0033 | 0.0017 | 0.0096 | 0.0034 | 0.004 | 0.0021 | 0.0033 | 0.0033 | 0.003 | 0.0033 | 0.0045 | 0.0055 | 0.0065 | 0.0053 | 0.0098 | 0.0061 | 0.0072 |
Peroxisomes | 0.0004 | 0.0007 | 0.0027 | 0.0015 | 0.0041 | 0.0005 | 0.0083 | 0.0007 | 0.0007 | 0.0361 | 0.0014 | 0.0028 | 0.0056 | 0.0002 | 0.0013 | 0.0027 | 0.0088 | 0.0021 |
Punctate Nuclear | 0.0027 | 0.0049 | 0.0034 | 0.0307 | 0.0029 | 0.002 | 0.0021 | 0.0043 | 0.0021 | 0.0024 | 0.0027 | 0.0009 | 0.0096 | 0.0028 | 0.0055 | 0.0038 | 0.0069 | 0.005 |
Vacuole | 0.0125 | 0.0073 | 0.0084 | 0.0052 | 0.0053 | 0.0063 | 0.0089 | 0.0112 | 0.0067 | 0.0062 | 0.0148 | 0.0054 | 0.0137 | 0.0137 | 0.0092 | 0.0091 | 0.009 | 0.0065 |
Vacuole Periphery | 0.0005 | 0.0007 | 0.0007 | 0.0009 | 0.0007 | 0.0008 | 0.0017 | 0.0007 | 0.001 | 0.0013 | 0.0062 | 0.0042 | 0.0029 | 0.0013 | 0.0014 | 0.0025 | 0.0024 | 0.001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 16.6783 | 15.6941 | 9.8989 | 16.6796 | 15.3646 | 20.1784 | 16.0667 | 18.2071 | 16.7717 | 21.1876 |
Translational Efficiency | 1.1363 | 0.9483 | 1.1121 | 0.9157 | 1.0166 | 1.0409 | 0.9758 | 0.8627 | 1.1123 | 0.7612 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
645 | 915 | 1709 | 1505 | 1319 | 1747 | 1271 | 1223 | 1964 | 2662 | 2980 | 2728 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 676.68 | 798.47 | 933.26 | 1000.25 | 723.17 | 772.38 | 936.99 | 1013.02 | 707.90 | 781.35 | 934.85 | 1005.97 |
Standard Deviation | 83.02 | 119.88 | 114.47 | 144.71 | 93.12 | 100.71 | 118.72 | 133.34 | 92.54 | 108.39 | 116.32 | 139.87 |
Intensity Change Log 2 | — | 0.238764 | 0.463805 | 0.563815 | — | 0.094976 | 0.373699 | 0.486256 | — | 0.166273 | 0.417959 | 0.524268 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000756 | 0.002504 | 0.002506 | 0.048817 | 0.000598 | 0.000727 | 0.006222 | 0.032594 | 0.000650 | 0.001337 | 0.004091 | 0.041544 |
Bud Neck | 0.014145 | 0.010512 | 0.012037 | 0.022008 | 0.007780 | 0.011829 | 0.012107 | 0.020231 | 0.009871 | 0.011377 | 0.012067 | 0.021211 |
Bud Site | 0.006566 | 0.015151 | 0.013789 | 0.071717 | 0.005902 | 0.009038 | 0.015218 | 0.044897 | 0.006120 | 0.011139 | 0.014399 | 0.059694 |
Cell Periphery | 0.001179 | 0.000913 | 0.000902 | 0.003355 | 0.000891 | 0.000690 | 0.000711 | 0.002169 | 0.000986 | 0.000767 | 0.000820 | 0.002823 |
Cytoplasm | 0.347034* | 0.296940* | 0.331750* | 0.132421* | 0.424000* | 0.348653* | 0.239768* | 0.140804* | 0.398724* | 0.330878* | 0.292519* | 0.136179* |
Cytoplasmic Foci | 0.214461* | 0.208322* | 0.081076 | 0.110879 | 0.159868 | 0.192546* | 0.157386 | 0.129684 | 0.177797 | 0.197969* | 0.113623 | 0.119310 |
Eisosomes | 0.001547 | 0.001370 | 0.000309 | 0.002342 | 0.001074 | 0.000972 | 0.000496 | 0.002099 | 0.001229 | 0.001109 | 0.000389 | 0.002233 |
Endoplasmic Reticulum | 0.110022* | 0.162722* | 0.086403 | 0.132016* | 0.088865 | 0.106454* | 0.057296 | 0.096353 | 0.095813 | 0.125795* | 0.073989 | 0.116028* |
Endosome | 0.018624 | 0.028913 | 0.026942 | 0.091138 | 0.012182 | 0.024172 | 0.057916 | 0.092500 | 0.014297 | 0.025802 | 0.040153 | 0.091749 |
Golgi | 0.012854 | 0.015291 | 0.002775 | 0.034087 | 0.010103 | 0.019301 | 0.008357 | 0.030522 | 0.011007 | 0.017923 | 0.005156 | 0.032489 |
Lipid Particles | 0.080721 | 0.072452 | 0.060074 | 0.145901* | 0.067611 | 0.095537 | 0.135456* | 0.151639* | 0.071916 | 0.087602 | 0.092225 | 0.148474* |
Mitochondria | 0.010013 | 0.006331 | 0.001088 | 0.005002 | 0.008899 | 0.010398 | 0.001469 | 0.004330 | 0.009265 | 0.009000 | 0.001251 | 0.004701 |
Mitotic Spindle | 0.001243 | 0.002441 | 0.010145 | 0.020653 | 0.001977 | 0.002488 | 0.022857 | 0.041101 | 0.001736 | 0.002472 | 0.015567 | 0.029820 |
None | 0.002777 | 0.002438 | 0.002471 | 0.000382 | 0.002733 | 0.001909 | 0.001971 | 0.000448 | 0.002748 | 0.002091 | 0.002258 | 0.000411 |
Nuclear Periphery | 0.003207 | 0.003392 | 0.007884 | 0.000968 | 0.003375 | 0.002778 | 0.005862 | 0.001552 | 0.003320 | 0.002989 | 0.007022 | 0.001230 |
Nuclear Periphery Foci | 0.005156 | 0.012343 | 0.051783 | 0.022343 | 0.004278 | 0.004618 | 0.051138 | 0.026379 | 0.004566 | 0.007274 | 0.051508 | 0.024153 |
Nucleolus | 0.000823 | 0.001118 | 0.002120 | 0.000362 | 0.000743 | 0.001198 | 0.002828 | 0.000923 | 0.000769 | 0.001170 | 0.002422 | 0.000613 |
Nucleus | 0.112685 | 0.063582 | 0.188649* | 0.025021 | 0.150794* | 0.091303 | 0.081906 | 0.021123 | 0.138278* | 0.081774 | 0.143122* | 0.023274 |
Peroxisomes | 0.008558 | 0.009009 | 0.004661 | 0.014626 | 0.004825 | 0.006876 | 0.017833 | 0.015299 | 0.006051 | 0.007609 | 0.010279 | 0.014928 |
Vacuole | 0.045729 | 0.080696 | 0.112222 | 0.113816 | 0.042318 | 0.066143 | 0.121878 | 0.141975 | 0.043438 | 0.071145 | 0.116340 | 0.126440 |
Vacuole Periphery | 0.001900 | 0.003561 | 0.000413 | 0.002145 | 0.001185 | 0.002368 | 0.001325 | 0.003376 | 0.001420 | 0.002778 | 0.000802 | 0.002697 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -3.24 | -4.47 | -14.21 | -13.50 | -13.54 | -0.89 | -4.93 | -9.93 | -9.89 | -7.74 | -3.29 | -6.59 | -17.31 | -16.95 | -15.46 |
Bud Neck | 1.53 | 0.57 | -3.20 | -7.26 | -6.15 | -3.36 | -3.36 | -6.81 | -4.45 | -4.04 | -1.36 | -2.44 | -8.46 | -8.08 | -7.17 |
Bud Site | -4.06 | -4.37 | -17.69 | -14.19 | -15.09 | -2.29 | -4.96 | -11.47 | -10.29 | -7.97 | -4.34 | -6.76 | -21.08 | -18.38 | -16.73 |
Cell Periphery | 1.20 | 1.39 | -5.85 | -7.90 | -8.16 | 2.81 | 1.78 | -3.40 | -4.06 | -3.93 | 2.51 | 2.03 | -7.48 | -8.87 | -8.60 |
Cytoplasm | 4.24 | 2.66 | 20.71 | 17.68 | 22.55 | 8.79 | 18.96 | 31.71 | 24.96 | 9.87 | 9.69 | 15.93 | 39.58 | 32.22 | 23.28 |
Cytoplasmic Foci | 0.68 | 17.29 | 13.66 | 14.52 | -5.37 | -5.56 | 0.37 | 5.29 | 11.00 | 4.64 | -4.00 | 13.74 | 13.30 | 18.81 | -0.51 |
Eisosomes | 0.42 | 4.99 | -1.77 | -1.89 | -7.18 | 0.86 | 5.82 | -1.95 | -2.22 | -3.52 | 0.74 | 8.14 | -3.24 | -3.56 | -7.27 |
Endoplasmic Reticulum | -4.47 | 3.30 | -1.56 | 3.63 | -6.55 | -2.57 | 5.89 | 0.20 | 2.63 | -5.31 | -4.95 | 5.44 | -2.16 | 2.94 | -8.26 |
Endosome | -4.58 | -3.88 | -16.65 | -14.62 | -15.06 | -8.63 | -14.85 | -17.73 | -14.64 | -6.16 | -9.66 | -14.97 | -25.28 | -21.12 | -15.15 |
Golgi | -1.63 | 9.14 | -8.34 | -7.36 | -14.91 | -6.74 | 2.58 | -7.74 | -3.12 | -8.95 | -6.71 | 9.00 | -12.00 | -6.87 | -17.15 |
Lipid Particles | 1.48 | 4.39 | -8.77 | -10.59 | -13.65 | -6.32 | -9.13 | -10.53 | -6.45 | -1.67 | -4.49 | -3.83 | -14.43 | -11.23 | -10.63 |
Mitochondria | 1.80 | 4.70 | 2.56 | 1.38 | -3.72 | -0.79 | 4.60 | 2.82 | 5.16 | -3.13 | 0.18 | 6.39 | 3.58 | 4.98 | -4.84 |
Mitotic Spindle | -0.81 | -5.74 | -7.57 | -7.15 | -3.65 | -0.48 | -7.32 | -9.71 | -9.64 | -3.76 | -0.85 | -9.27 | -12.25 | -12.03 | -5.31 |
None | 0.23 | 0.26 | 1.92 | 2.95 | 3.68 | 1.47 | 0.89 | 4.62 | 4.34 | 2.95 | 1.08 | 0.72 | 4.46 | 5.22 | 4.70 |
Nuclear Periphery | -0.21 | -6.67 | 3.95 | 2.68 | 11.05 | 1.42 | -3.85 | 4.28 | 3.61 | 7.14 | 0.80 | -7.80 | 6.05 | 4.57 | 13.12 |
Nuclear Periphery Foci | -6.67 | -17.11 | -11.18 | -5.68 | 9.61 | -0.75 | -15.19 | -11.44 | -11.18 | 6.67 | -5.37 | -23.02 | -16.20 | -13.46 | 11.57 |
Nucleolus | -1.24 | -3.44 | 2.89 | 3.04 | 3.96 | -1.75 | -3.55 | -0.50 | 0.77 | 3.07 | -2.12 | -4.83 | 0.87 | 2.42 | 4.97 |
Nucleus | 7.57 | -9.90 | 14.22 | 9.62 | 29.62 | 11.22 | 11.65 | 26.88 | 22.39 | 14.82 | 13.55 | -0.89 | 29.65 | 23.04 | 32.04 |
Peroxisomes | -0.41 | 4.82 | -4.02 | -3.27 | -7.52 | -3.95 | -8.15 | -8.32 | -6.65 | 1.33 | -3.05 | -5.23 | -9.44 | -7.43 | -3.94 |
Vacuole | -7.31 | -16.42 | -15.55 | -8.92 | -1.55 | -7.38 | -17.34 | -18.73 | -15.15 | -4.01 | -10.34 | -24.71 | -24.74 | -17.96 | -4.04 |
Vacuole Periphery | -3.82 | 7.43 | -0.18 | 2.78 | -3.84 | -5.97 | -0.00 | -2.54 | -0.83 | -2.19 | -6.87 | 3.41 | -2.54 | 0.83 | -3.97 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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