Standard name
Human Ortholog
Description Protein of unknown function that associates with ribosomes
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.08 0.05 0 0 0 0 0 0 0 0 0 0 0.07 0.13 0.15 0.06 0.13 0.11 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.15 0.11 0.09 0.29 0.36 0.3 0.41 0.33 0.4 0.42 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.93 0.93 0.94 0.89 0.9 0.85 0.78 0.79 0.8 0.79 0.77 0.77 0.92 0.76 0.67 0.79 0.78 0.83 0.95 0.92 0.95 0.81 0.76 0.69
Nuclear Periphery 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0.08 0.08 0.09 0.14 0.13 0.14 0.18 0.15 0.14 0 0.11 0.19 0.05 0 0 0 0 0 0 0 0.06
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0 0.05 0 0 0 0.09 0.14 0.22
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 2 0
Bud 0 1 1 0 0 2 1 4 2 9 8 5 0 0 1 0 0 0 2 2 1 0 3 2
Bud Neck 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 2 1 6 11 12 24 24 17 35 33 0 2 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Cytoplasm 25 31 7 12 11 10 16 26 9 9 13 12 18 42 50 9 27 14 1 4 3 1 1 1
Endoplasmic Reticulum 0 0 0 1 0 0 0 0 0 1 0 0 0 0 1 2 2 0 0 1 0 1 1 0
Endosome 0 1 0 0 1 0 0 0 0 0 0 0 1 0 3 0 1 0 1 3 0 0 2 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 1 1
Mitochondria 2 3 55 45 43 147 307 253 248 276 297 401 0 0 1 0 8 1 1 3 1 1 1 0
Nucleus 299 598 347 370 452 426 669 652 489 655 571 743 251 236 229 112 157 106 309 615 352 108 220 180
Nuclear Periphery 1 1 1 7 25 16 29 19 23 17 23 24 0 3 2 1 3 1 0 0 0 1 0 0
Nucleolus 1 4 7 34 41 44 119 109 83 146 112 138 3 34 64 7 6 3 0 2 0 2 7 16
Peroxisomes 0 0 0 0 0 0 0 0 1 0 0 0 0 4 2 0 0 0 0 0 0 0 0 0
SpindlePole 0 1 0 0 0 0 1 1 0 1 1 1 1 6 7 1 0 0 0 3 0 0 0 0
Vac/Vac Membrane 4 16 5 3 4 1 8 14 6 4 6 11 2 6 5 12 7 6 7 23 10 12 41 58
Unique Cell Count 322 644 370 417 505 502 854 830 611 825 746 963 273 312 341 141 201 128 324 671 372 134 290 263
Labelled Cell Count 332 656 425 474 583 657 1162 1102 885 1135 1066 1368 276 333 366 144 211 131 324 671 372 134 290 263


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.5 12.9 6.1 7.0 6.4 4.9 4.6 4.9 4.2 4.7 4.4 4.5 11.7 11.0 9.9 17.8 17.6 17.2 12.4 13.3 13.6
Std Deviation (1e-4) 4.3 4.1 1.3 1.8 1.3 1.1 1.2 1.3 1.0 1.3 1.1 1.1 3.0 3.6 3.0 5.2 6.1 5.9 3.0 3.4 2.9
Intensity Change (Log2) – – – 0.19 0.07 -0.33 -0.4 -0.33 -0.52 -0.39 -0.46 -0.45 0.94 0.85 0.7 1.55 1.53 1.49 1.03 1.12 1.16


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.9 0.3 0.1 0 1.2 -0.5 0 -0.2 -0.9 3.0 5.8 6.3 0 5.6 4.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -1.7 -2.9 0 0 0 0 0 0 0 -6.7 -7.1 -7.2 -4.8 -4.0 -4.3
Nucleus -2.5 -2.2 -4.1 -6.6 -6.5 -5.9 -6.2 -7.1 -7.0 -0.9 -6.7 -9.0 -4.8 -5.6 -3.7
Nuclear Periphery 0 4.0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 3.9 4.0 0 0 0 0 0 0 0 0 4.9 7.5 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0.4 0 0 0 0 0 0.6 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.7965 6.4182 5.5208 5.5394 4.836 5.4901 5.5389 5.915 5.6166 4.5617 5.1146 5.3356 7.6485 7.0646 6.9264 6.7684 6.6038 6.7255
Actin 0.0265 0.0001 0.0015 0 0.0461 0 0.006 0 0.0014 0 0.0095 0.0002 0.0002 0 0 0 0 0
Bud 0.0013 0.0003 0.0001 0 0.0043 0 0.0004 0 0.0002 0.0001 0.0003 0 0 0 0 0 0 0
Bud Neck 0.0062 0.0004 0.0002 0 0.0021 0.0006 0.002 0 0.0003 0 0.0006 0.0004 0.0001 0 0 0 0.0001 0.0003
Bud Periphery 0.0026 0.0003 0.0004 0 0.004 0 0.0011 0 0.0004 0.0003 0.001 0.0001 0 0 0 0 0 0
Bud Site 0.0067 0.0042 0.0002 0 0.0399 0 0.0048 0 0.0006 0.0001 0.0003 0 0 0 0 0 0 0
Cell Periphery 0.0009 0 0.0001 0 0.0006 0 0.0005 0 0.0002 0.0002 0.0001 0 0 0 0 0 0 0
Cytoplasm 0.0039 0.0001 0.0004 0 0.0016 0 0.003 0 0.0004 0.0001 0.0003 0 0.0001 0 0 0 0 0
Cytoplasmic Foci 0.0386 0.0048 0.0023 0 0.0277 0 0.0149 0 0.0049 0.0007 0.0002 0.0001 0.0002 0 0 0 0 0
Eisosomes 0.0008 0 0 0 0.0003 0 0.0001 0 0 0 0.0001 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0031 0 0.0008 0 0.0007 0.0001 0.0034 0 0.0007 0.0001 0.0019 0.0001 0 0 0 0 0 0
Endosome 0.0147 0.0011 0.0038 0 0.0221 0 0.0133 0 0.003 0.002 0.0025 0.0004 0.0001 0 0 0 0 0
Golgi 0.0061 0.0004 0.0046 0 0.0388 0 0.0028 0 0.001 0.0003 0.0008 0.0004 0.0001 0 0 0 0 0
Lipid Particles 0.057 0.0064 0.0024 0 0.0051 0 0.0089 0 0.0033 0.0058 0.0006 0.0034 0.0008 0 0 0 0 0
Mitochondria 0.0142 0.0003 0.0091 0 0.0405 0.0001 0.0053 0.0001 0.0057 0.0032 0.0089 0.0004 0.0003 0.0001 0 0 0.0001 0
None 0.018 0.0001 0.0001 0 0.0003 0 0.002 0 0.0001 0.0001 0.0002 0 0 0 0 0 0 0
Nuclear Periphery 0.0089 0.0006 0.0032 0.0003 0.0014 0.0052 0.023 0.0032 0.0053 0.0006 0.0073 0.0021 0.0008 0.0031 0.0011 0.0002 0.0018 0.0006
Nucleolus 0.018 0.0044 0.0025 0.0022 0.073 0.0141 0.0207 0.0089 0.0067 0.0041 0.0396 0.0246 0.006 0.0064 0.0076 0.0018 0.0183 0.0228
Nucleus 0.7356 0.9721 0.9612 0.9973 0.6739 0.9792 0.859 0.9819 0.9583 0.9724 0.9235 0.9663 0.9868 0.9895 0.9911 0.9978 0.9793 0.9758
Peroxisomes 0.0127 0.0018 0.0011 0 0.0128 0 0.0055 0 0.0014 0.0003 0.0002 0.0003 0.0042 0 0 0 0 0
Punctate Nuclear 0.016 0.0023 0.0001 0 0.0007 0.0001 0.0119 0.0057 0.0035 0.0001 0.0007 0.0006 0.0001 0.0007 0.0001 0 0.0005 0.0002
Vacuole 0.0059 0.0003 0.0029 0 0.0023 0.0002 0.0079 0 0.0018 0.0061 0.0007 0.0003 0.0001 0 0 0 0 0
Vacuole Periphery 0.0025 0 0.0029 0 0.0017 0.0001 0.0035 0.0001 0.0009 0.0035 0.0007 0.0002 0.0001 0.0001 0 0 0 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 26.7118 20.2051 42.2532 24.552 31.969 28.7507 25.5367 26.3234 17.6665 29.8228
Translational Efficiency 3.907 4.3304 1.8444 2.54 2.8183 2.781 2.4143 1.8325 2.7059 2.4883

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2365 1577 1558 1156 2184 1263 2009 1729 4549 2840 3567 2885

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 952.59 965.29 1179.74 1288.73 919.44 1131.09 1289.11 1291.39 936.67 1039.02 1241.34 1290.32
Standard Deviation 202.07 199.55 197.92 243.40 170.56 184.73 227.57 286.20 188.33 209.95 221.86 269.87
Intensity Change Log 2 — 0.019107 0.308542 0.436023 — 0.298886 0.487548 0.490097 — 0.163297 0.399236 0.462835

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000258 0.000403 0.000523 0.000811 0.000293 0.000528 0.000415 0.000686 0.000275 0.000459 0.000462 0.000736
Bud Neck 0.003484 0.003451 0.010995 0.022695 0.003991 0.013417 0.010487 0.022792 0.003727 0.007883 0.010709 0.022753
Bud Site 0.000807 0.000458 0.001390 0.002318 0.000440 0.001535 0.000443 0.003183 0.000631 0.000937 0.000856 0.002836
Cell Periphery 0.000830 0.000396 0.000106 0.000130 0.000330 0.000383 0.000341 0.000229 0.000590 0.000390 0.000238 0.000189
Cytoplasm 0.003797 0.000766 0.000441 0.002217 0.000376 0.000756 0.000092 0.004269 0.002155 0.000762 0.000245 0.003447
Cytoplasmic Foci 0.000357 0.000043 0.000016 0.000586 0.000042 0.000255 0.000024 0.000720 0.000206 0.000137 0.000020 0.000666
Eisosomes 0.000080 0.000044 0.000048 0.000033 0.000051 0.000056 0.000068 0.000040 0.000066 0.000049 0.000059 0.000037
Endoplasmic Reticulum 0.009883 0.005828 0.002826 0.002471 0.008556 0.009756 0.002172 0.002455 0.009246 0.007575 0.002458 0.002461
Endosome 0.000226 0.000348 0.000072 0.001260 0.000192 0.001024 0.000087 0.001314 0.000210 0.000649 0.000080 0.001292
Golgi 0.000127 0.000049 0.000028 0.001234 0.000106 0.000080 0.000014 0.000444 0.000117 0.000063 0.000020 0.000761
Lipid Particles 0.000070 0.000027 0.000018 0.000333 0.000022 0.000218 0.000074 0.000215 0.000047 0.000112 0.000050 0.000262
Mitochondria 0.000344 0.000569 0.000952 0.004222 0.000734 0.001559 0.000939 0.002324 0.000531 0.001009 0.000944 0.003085
Mitotic Spindle 0.000071 0.000117 0.000097 0.012358 0.000597 0.003100 0.000266 0.007914 0.000324 0.001444 0.000192 0.009694
None 0.009440 0.002474 0.001515 0.002211 0.001840 0.001955 0.000378 0.002358 0.005791 0.002243 0.000875 0.002299
Nuclear Periphery 0.000263 0.000162 0.000158 0.001309 0.000304 0.000639 0.000127 0.000921 0.000282 0.000374 0.000141 0.001076
Nuclear Periphery Foci 0.000466 0.000074 0.000026 0.001082 0.000117 0.000470 0.000008 0.000492 0.000298 0.000250 0.000016 0.000728
Nucleolus 0.006588 0.005551 0.004894 0.014429 0.006129 0.008099 0.004107 0.009302 0.006368 0.006684 0.004451 0.011357
Nucleus 0.960722* 0.978164* 0.975102* 0.925874* 0.974390* 0.951855* 0.979253* 0.936058* 0.967284* 0.966464* 0.977440* 0.931978*
Peroxisomes 0.000222 0.000128 0.000172 0.000448 0.000101 0.000291 0.000122 0.000280 0.000164 0.000200 0.000144 0.000347
Vacuole 0.001779 0.000780 0.000510 0.002986 0.001082 0.002520 0.000490 0.003013 0.001444 0.001554 0.000499 0.003002
Vacuole Periphery 0.000185 0.000169 0.000112 0.000991 0.000308 0.001503 0.000095 0.000992 0.000244 0.000762 0.000102 0.000992

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -8.16 -10.34 -14.57 -11.32 -7.92 -5.16 -3.06 -12.14 -4.12 -6.59 -8.05 -7.57 -18.87 -10.70 -9.91
Bud Neck 0.03 -9.59 -14.59 -14.63 -8.78 -8.06 -8.14 -14.89 -6.99 -9.50 -7.48 -12.47 -20.72 -15.52 -12.84
Bud Site 1.72 -1.10 -4.65 -6.34 -2.19 -4.33 -0.17 -6.21 -3.35 -6.18 -1.84 -0.94 -7.41 -6.48 -5.81
Cell Periphery 2.32 4.40 4.33 3.04 -0.44 -0.45 -0.01 2.01 1.68 0.74 1.81 2.74 4.60 3.13 0.65
Cytoplasm 3.64 4.54 2.09 -2.08 -3.45 -1.60 2.51 -4.47 -3.93 -4.82 3.06 4.91 -1.77 -4.34 -5.67
Cytoplasmic Foci 2.72 2.99 0.03 -2.15 -2.35 -1.70 0.78 -1.48 -0.85 -1.53 1.00 3.07 -1.18 -1.56 -2.12
Eisosomes 4.62 4.22 6.16 3.29 5.92 -0.69 -2.57 2.54 3.02 5.48 3.25 1.31 6.40 4.00 6.98
Endoplasmic Reticulum 6.91 12.55 11.86 7.13 0.09 -1.59 14.54 13.84 11.21 -1.70 3.58 19.03 18.20 13.82 -0.85
Endosome -0.57 4.85 -2.38 -1.60 -2.95 -2.63 1.65 -2.54 -0.12 -2.80 -2.42 3.37 -3.33 -1.20 -3.86
Golgi 0.82 1.04 -1.39 -1.59 -1.63 0.36 1.50 -1.36 -1.54 -1.91 0.91 1.66 -1.86 -2.17 -2.37
Lipid Particles 1.11 1.39 -1.32 -1.73 -1.80 -1.95 -0.90 -2.04 0.32 -1.22 -1.31 0.02 -2.23 -1.12 -2.15
Mitochondria -3.81 -9.16 -5.91 -5.54 -4.88 -2.68 -1.33 -7.22 -2.75 -7.11 -3.33 -4.78 -9.04 -6.90 -7.59
Mitotic Spindle -0.90 -0.57 -4.65 -4.60 -4.64 -2.10 0.74 -5.15 -2.82 -5.43 -2.15 0.56 -7.01 -5.68 -7.13
None 4.96 5.76 5.19 0.20 -1.74 -0.17 3.37 -1.22 -0.73 -9.04 4.37 6.64 4.54 -0.39 -6.17
Nuclear Periphery 2.54 2.51 -4.83 -5.28 -5.27 -3.72 4.59 -4.58 -2.01 -5.85 -2.12 5.05 -6.70 -5.79 -7.83
Nuclear Periphery Foci 2.67 3.12 -0.81 -1.89 -2.04 -2.05 1.71 -2.52 0.24 -3.80 0.35 3.56 -1.52 -1.74 -3.40
Nucleolus 1.18 1.98 -5.92 -6.27 -6.54 -2.02 2.65 -4.75 -3.14 -5.88 -0.65 3.37 -7.49 -6.94 -8.75
Nucleus -6.71 -5.07 8.11 11.92 11.10 6.14 -2.34 11.46 4.71 12.53 0.54 -5.80 13.28 12.24 16.68
Peroxisomes 0.70 0.37 -1.15 -3.25 -2.72 -2.70 -0.86 -3.48 -0.14 -2.95 -0.35 0.29 -2.00 -2.63 -3.98
Vacuole 4.75 5.90 -2.06 -3.76 -4.15 -3.26 4.30 -3.22 -0.81 -4.20 -0.41 7.32 -3.62 -3.25 -5.81
Vacuole Periphery 0.30 1.78 -3.06 -3.06 -3.40 -1.95 3.58 -2.19 1.00 -3.12 -1.89 4.06 -3.46 -0.17 -4.35
Standard name
Human Ortholog
Description Protein of unknown function that associates with ribosomes
Localization
Cell Percentages nucleus (100%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Tma16

Tma16


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tma16-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available