Standard name
Human Ortholog
Description Subunit of protein N-terminal acetyltransferase NatA; NatA is comprised of Nat1p, Ard1p, and Nat5p; N-terminally acetylates many proteins, which influences multiple processes such as the cell cycle, heat-shock resistance, mating, sporulation, and telomeric silencing
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0
Cytoplasm 0.96 0.96 0.97 0.97 0.95 0.9 0.89 – 0.75 0.73 0.68 0.6 0.98 0.99 0.99 0.93 0.95 0.92 0.94 0.94 0.88 0.89 0.88 0.88
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.26 0.08 – 0.34 0.27 0.53 0.5 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.05 0 0 – 0.05 0 0 0 0 0 0 0 0.05 0.07 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 2 – 1 0 2 1 1 0 0 0 0 0 0 1 0 0 2 2
Bud 0 1 0 1 1 4 0 – 4 3 6 6 2 0 0 0 0 0 1 0 1 0 3 6
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 3 2 5 3 8 3 11 – 4 17 5 1 4 9 7 6 8 3 0 0 0 1 0 0
Cytoplasm 383 137 183 289 247 247 375 – 203 294 102 96 324 543 630 197 117 154 377 136 174 272 384 380
Endoplasmic Reticulum 9 3 4 1 0 2 3 – 1 5 0 7 4 4 1 7 3 3 3 0 5 14 21 16
Endosome 2 0 0 0 2 1 2 – 1 3 2 0 1 0 0 0 0 1 2 0 1 0 1 2
Golgi 2 0 0 0 0 0 1 – 0 0 0 0 0 0 0 1 0 1 4 0 0 0 0 2
Mitochondria 3 3 2 10 5 70 35 – 91 109 80 80 2 1 3 3 1 3 2 1 4 6 5 5
Nucleus 2 1 1 2 1 1 5 – 2 4 1 3 1 0 1 6 0 1 1 0 0 0 0 1
Nuclear Periphery 0 2 1 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Nucleolus 0 0 0 0 0 1 0 – 0 0 0 1 0 0 1 0 0 0 0 0 0 1 0 1
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 1 0 4 – 1 6 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Vac/Vac Membrane 2 0 2 5 13 1 17 – 14 15 3 2 3 1 3 7 6 12 4 3 5 2 9 5
Unique Cell Count 397 142 188 297 261 274 420 270 400 151 160 329 546 637 211 123 167 403 146 198 306 435 432
Labelled Cell Count 406 149 198 311 278 331 455 322 456 201 197 342 558 646 227 135 178 403 146 198 306 435 432


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.3 6.7 7.1 7.0 7.2 5.3 6.3 – 5.6 5.9 5.3 5.0 8.0 8.2 8.5 9.8 9.9 10.0 7.6 7.9 8.2
Std Deviation (1e-4) 1.1 1.2 1.5 1.5 1.4 1.7 1.5 – 1.4 1.3 1.4 0.8 1.4 1.6 1.6 2.2 2.0 1.8 1.5 1.9 2.1
Intensity Change (Log2) – – – -0.02 0.02 -0.43 -0.18 – -0.35 -0.27 -0.43 -0.51 0.16 0.2 0.26 0.46 0.47 0.48 0.09 0.15 0.2


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0.3 0 0 – 0 0.9 0 0 0 0 0 0.1 1.7 0
Cytoplasm 0 -1.4 -3.0 -3.3 – -6.4 -6.9 -7.4 -8.7 0.9 2.4 1.6 -1.9 -1.0 -2.2
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 1.2 0 0 0 0.7 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 7.1 3.5 – 8.5 7.6 11.1 10.7 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 2.3 0 2.0 – 2.4 1.8 0 0 0 0 0 0 0 3.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.0148 3.1504 2.4999 2.2017 2.1466 2.5811 2.991 4.4112 3.9281 3.695 3.4138 4.1858 4.6462 5.9209 5.418 5.5345 5.9454 5.5377
Actin 0.0378 0.0025 0.0149 0.0014 0.0009 0.0084 0.0189 0.0031 0.0003 0.0001 0.0134 0.0033 0.0264 0.0009 0.0172 0.0005 0.0015 0.0152
Bud 0.0016 0.004 0.006 0.0017 0.0013 0.0014 0.0005 0.0005 0.0009 0.0003 0.0005 0.0002 0.0004 0.001 0.0006 0.0004 0.0008 0.0002
Bud Neck 0.0105 0.0005 0.0009 0.0006 0.0032 0.0013 0.0018 0.0002 0.0003 0.0003 0.0005 0.0019 0.0004 0.0004 0.0025 0.0004 0.0005 0.0005
Bud Periphery 0.0024 0.0038 0.0077 0.0008 0.0018 0.0018 0.0006 0.0002 0.0006 0.0001 0.0003 0.0001 0.0006 0.0005 0.0004 0.0001 0.0042 0.0003
Bud Site 0.0078 0.0143 0.0076 0.0027 0.0004 0.0012 0.0038 0.0011 0.0016 0.0002 0.0007 0.0001 0.0008 0.0271 0.0067 0.0004 0.0004 0.0002
Cell Periphery 0.0005 0.0005 0.0004 0.0001 0.0001 0.0001 0.0004 0.0001 0.0001 0 0.0001 0 0.0003 0.0006 0.0002 0.0001 0.0002 0.0001
Cytoplasm 0.6936 0.8782 0.8087 0.7681 0.8304 0.8372 0.7744 0.9326 0.8894 0.9137 0.7675 0.9 0.8066 0.8838 0.8821 0.8799 0.7445 0.8842
Cytoplasmic Foci 0.0333 0.0323 0.0377 0.057 0.0368 0.0314 0.0324 0.0134 0.0236 0.0219 0.0359 0.0189 0.0309 0.016 0.0128 0.0209 0.0353 0.0151
Eisosomes 0.0005 0 0.0001 0 0 0.0001 0.0003 0 0 0 0.0001 0 0.0003 0 0.0001 0 0.0001 0.0001
Endoplasmic Reticulum 0.0184 0.0037 0.0089 0.0028 0.0043 0.0041 0.019 0.0034 0.0035 0.0026 0.0093 0.009 0.0109 0.002 0.0045 0.0039 0.0053 0.0065
Endosome 0.0724 0.0185 0.0424 0.1074 0.0802 0.055 0.0447 0.0184 0.0307 0.0394 0.0748 0.04 0.0311 0.0217 0.0218 0.0643 0.0672 0.0218
Golgi 0.0125 0.0019 0.0066 0.0067 0.0033 0.0089 0.0154 0.0036 0.0012 0.0009 0.0146 0.0028 0.0093 0.0008 0.0063 0.002 0.0075 0.0101
Lipid Particles 0.0078 0.0004 0.0079 0.0004 0.0006 0.0038 0.0068 0.0002 0.0002 0.0001 0.0122 0.001 0.0085 0.0001 0.0034 0.0001 0.0082 0.0052
Mitochondria 0.0173 0.0009 0.0038 0.0015 0.0011 0.0026 0.0122 0.0017 0.0067 0.0004 0.0089 0.0015 0.0045 0.0001 0.0015 0.0007 0.0462 0.0062
None 0.0463 0.0304 0.0268 0.026 0.0086 0.0226 0.0435 0.0139 0.0303 0.008 0.0404 0.014 0.0351 0.0411 0.0282 0.0093 0.0565 0.025
Nuclear Periphery 0.0095 0.0004 0.0012 0.001 0.0049 0.0038 0.0044 0.0005 0.0008 0.0007 0.0022 0.0009 0.0044 0.0003 0.0017 0.0008 0.0015 0.0016
Nucleolus 0.0007 0.0002 0.0001 0.0001 0.0002 0.0009 0.0004 0 0.0001 0 0.0001 0 0.0001 0 0.0002 0 0.0002 0.0001
Nucleus 0.0036 0.0009 0.0013 0.0013 0.0099 0.0027 0.0014 0.0009 0.0011 0.001 0.0013 0.0007 0.0221 0.0007 0.001 0.0008 0.0008 0.0005
Peroxisomes 0.0079 0.0007 0.0085 0.001 0.0002 0.0012 0.0092 0.0004 0.0002 0.0001 0.0063 0.0002 0.0021 0.0001 0.0013 0.0001 0.009 0.0041
Punctate Nuclear 0.0037 0.0007 0.0007 0.0006 0.0009 0.0059 0.002 0.0001 0.0004 0.0002 0.0011 0.0002 0.0013 0.0001 0.0034 0.0002 0.0058 0.0006
Vacuole 0.0084 0.0046 0.0066 0.0151 0.0087 0.0043 0.0065 0.005 0.0069 0.0086 0.0069 0.0036 0.003 0.0022 0.0033 0.0107 0.0031 0.0017
Vacuole Periphery 0.0035 0.0006 0.0012 0.0038 0.0021 0.0013 0.0015 0.0008 0.0012 0.0016 0.003 0.0013 0.0009 0.0003 0.0008 0.0044 0.0014 0.0008

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 68.5732 50.1547 51.001 74.8618 68.7017 45.6901 55.4069 56.4108 47.6429 73.0243
Translational Efficiency 1.72 2.0602 1.8149 0.954 1.4036 2.4037 2.0302 1.5755 2.0616 1.8398

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
632 1089 315 133 1288 2334 325 1586 1920 3423 640 1719

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 751.99 876.51 1181.82 1151.07 784.46 930.51 1156.71 1101.39 773.77 913.33 1169.07 1105.23
Standard Deviation 97.80 135.43 145.40 143.08 105.46 121.66 146.07 171.38 104.12 128.69 146.28 169.88
Intensity Change Log 2 — 0.221057 0.652225 0.614190 — 0.246322 0.560255 0.489554 — 0.234012 0.606001 0.551901

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000254 0.000384 0.000542 0.000476 0.000146 0.000184 0.000355 0.000229 0.000182 0.000248 0.000447 0.000249
Bud Neck 0.013656 0.004678 0.001184 0.001865 0.004319 0.005558 0.001158 0.001403 0.007392 0.005278 0.001171 0.001439
Bud Site 0.019375 0.036078 0.013663 0.022946 0.011373 0.021901 0.014331 0.019441 0.014007 0.026412 0.014002 0.019712
Cell Periphery 0.000263 0.000139 0.000136 0.000131 0.000163 0.000109 0.000105 0.000061 0.000196 0.000119 0.000120 0.000067
Cytoplasm 0.502165* 0.627348* 0.786285* 0.411989* 0.606521* 0.679933* 0.776203* 0.822023* 0.572170* 0.663204* 0.781165* 0.790298*
Cytoplasmic Foci 0.296291* 0.202131* 0.071869 0.100135 0.234137* 0.168707 0.077900 0.055555 0.254596* 0.179341 0.074932 0.059005
Eisosomes 0.000148 0.000079 0.000015 0.000031 0.000071 0.000039 0.000014 0.000006 0.000096 0.000052 0.000014 0.000008
Endoplasmic Reticulum 0.002644 0.001592 0.000966 0.001027 0.003644 0.000982 0.000370 0.000166 0.003315 0.001176 0.000663 0.000233
Endosome 0.040573 0.026902 0.005947 0.015433 0.023547 0.013819 0.005078 0.003428 0.029152 0.017981 0.005506 0.004357
Golgi 0.010356 0.009440 0.001187 0.000907 0.008110 0.008266 0.000607 0.002122 0.008849 0.008639 0.000892 0.002028
Lipid Particles 0.007474 0.002121 0.001129 0.001770 0.004348 0.001448 0.000976 0.000424 0.005377 0.001662 0.001051 0.000529
Mitochondria 0.007320 0.001168 0.001942 0.000022 0.002451 0.001926 0.000013 0.000880 0.004053 0.001685 0.000962 0.000814
Mitotic Spindle 0.000556 0.001648 0.004656 0.000445 0.001892 0.004335 0.000411 0.008226 0.001452 0.003480 0.002500 0.007624
None 0.001012 0.001542 0.000597 0.000210 0.001934 0.001999 0.000673 0.000326 0.001630 0.001854 0.000636 0.000317
Nuclear Periphery 0.000128 0.000112 0.000170 0.000221 0.000141 0.000047 0.000072 0.000056 0.000137 0.000068 0.000120 0.000069
Nuclear Periphery Foci 0.000386 0.000778 0.005160 0.004212 0.000523 0.000210 0.005488 0.000707 0.000478 0.000390 0.005327 0.000978
Nucleolus 0.000369 0.000302 0.000058 0.000146 0.000271 0.000268 0.000076 0.000317 0.000303 0.000279 0.000067 0.000304
Nucleus 0.014013 0.007721 0.023928 0.012040 0.016497 0.012488 0.013407 0.007903 0.015679 0.010972 0.018585 0.008224
Peroxisomes 0.002800 0.002142 0.000328 0.000842 0.001192 0.001957 0.000397 0.000924 0.001721 0.002016 0.000363 0.000918
Vacuole 0.072978 0.067624 0.079677 0.424552* 0.072754 0.071784 0.102134 0.074660 0.072827 0.070460 0.091081 0.101732
Vacuole Periphery 0.007240 0.006069 0.000562 0.000601 0.005967 0.004040 0.000233 0.001139 0.006386 0.004686 0.000395 0.001098

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.04 -4.26 -0.95 0.42 2.82 -1.43 -3.85 -1.43 -0.39 2.46 -1.52 -6.18 -0.77 0.77 4.92
Bud Neck 6.11 8.67 8.39 6.91 -1.54 -2.37 11.13 9.64 8.67 -1.39 3.48 11.92 11.30 10.64 -1.27
Bud Site -6.22 2.57 0.68 5.02 -0.85 -8.11 -1.96 -3.63 2.86 -1.47 -9.98 -0.09 -1.93 5.79 -1.72
Cell Periphery 3.49 3.31 4.61 2.41 2.69 6.43 6.09 14.60 8.91 5.28 5.96 5.31 11.21 10.07 8.17
Cytoplasm -9.77 -15.40 5.19 10.44 14.45 -8.56 -10.24 -21.21 -15.44 -3.07 -12.60 -16.90 -22.80 -13.31 -0.68
Cytoplasmic Foci 10.10 25.56 20.56 13.05 -0.83 10.67 22.34 33.26 27.95 4.84 14.54 33.22 42.63 33.88 4.91
Eisosomes 1.48 14.42 12.45 1.01 -4.10 6.92 13.45 15.95 13.37 5.56 2.95 19.16 21.53 2.91 6.55
Endoplasmic Reticulum 2.07 3.44 4.11 2.38 1.31 3.81 4.84 5.17 4.57 4.83 4.29 5.38 6.57 6.26 5.96
Endosome 3.85 10.77 8.71 7.45 -3.02 7.37 14.14 16.80 18.08 3.14 7.57 17.06 19.03 20.45 3.87
Golgi 0.86 10.70 11.37 12.49 1.91 -0.27 18.13 10.11 11.00 -2.16 0.44 19.79 12.18 12.72 -1.57
Lipid Particles 5.56 6.96 6.78 1.83 -0.19 6.00 6.75 8.23 10.33 3.39 8.19 9.59 11.26 8.22 5.93
Mitochondria 3.34 2.46 3.97 8.13 1.01 0.98 5.47 2.85 2.35 -1.45 3.35 3.31 4.35 2.33 0.18
Mitotic Spindle -1.46 -3.13 0.64 1.76 3.32 -2.08 1.94 -3.28 -1.57 -4.56 -2.44 -1.29 -3.66 -2.04 -2.86
None -0.98 1.34 2.54 2.69 3.43 -0.11 2.32 2.85 4.61 2.60 -0.50 2.63 3.33 5.21 3.63
Nuclear Periphery 0.42 -2.45 -2.77 -3.06 -1.02 3.90 1.84 2.27 -2.17 0.88 3.42 -1.01 1.81 -1.77 3.04
Nuclear Periphery Foci -1.83 -10.55 -6.86 -5.74 2.77 4.85 -9.34 -2.44 -8.03 8.95 1.02 -13.98 -5.78 -6.03 12.54
Nucleolus 0.51 2.48 2.02 4.06 -2.86 0.08 6.40 -0.25 -0.27 -1.25 0.47 5.13 -0.00 -0.14 -1.31
Nucleus 5.08 -4.98 -0.53 -2.70 3.37 2.75 -0.23 3.29 0.75 3.43 4.43 -4.03 3.14 -1.10 6.31
Peroxisomes 1.91 10.46 8.78 5.94 -3.30 -2.66 7.04 2.80 4.16 -4.08 -1.32 12.36 6.84 5.79 -4.65
Vacuole 0.93 -3.24 -16.77 -17.03 -14.62 0.25 -5.13 -7.42 -8.37 1.47 0.73 -5.94 -12.42 -13.40 -2.86
Vacuole Periphery 1.17 7.63 7.84 10.63 0.68 3.09 9.98 8.31 10.06 -3.46 3.19 12.39 10.72 12.79 -2.57
Standard name
Human Ortholog
Description Subunit of protein N-terminal acetyltransferase NatA; NatA is comprised of Nat1p, Ard1p, and Nat5p; N-terminally acetylates many proteins, which influences multiple processes such as the cell cycle, heat-shock resistance, mating, sporulation, and telomeric silencing
Localization
Cell Percentages cytoplasm (96%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-4

Nat5

Nat5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Nat5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available