Standard name
Human Ortholog
Description Involved in nuclear migration, part of the dynein/dynactin pathway; targets dynein to microtubule tips, which is necessary for sliding of microtubules along bud cortex; serves at interface between dynein's ATPase site and its microtubule binding stalk, causing individual dynein motors to remain attached to microtubules for long periods; synthetic lethal with bni1; homolog of human LIS1, mutations in which cause the severe brain disorder lissencephaly
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.74 0.85 0.88 0.85 0.93 0.93 0.87 0.83 0.79 0.74 0.55 0.69 0.97 0.97 0.98 0.9 0.85 0.88 0.57 0.74 0.58 0.81 0.82 0.76
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.08 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.42 0 0.22 0.28 0.14 0.21 0.32 0.29 0.38 0.49 0.6 0.47 0 0 0 0 0 0 0.09 0 0.12 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.06 0.15 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.15 0.11 0.14 0.07 0.11 0 0 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 2 3 3 0 2 3
Bud 0 0 1 1 1 0 0 1 3 4 3 3 1 0 0 1 1 0 0 1 1 1 4 8
Bud Neck 0 0 0 0 1 0 0 2 0 2 0 0 0 1 0 4 4 2 1 3 1 1 1 4
Bud Site 0 0 0 0 0 0 0 2 1 2 2 2 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 1 1 0 1 0 0 0 0 2 3 0 0 0 0 1 0
Cytoplasm 104 225 127 93 170 101 169 186 172 140 68 108 347 475 481 483 401 399 89 217 89 198 274 205
Endoplasmic Reticulum 1 1 1 1 0 0 0 1 2 0 0 0 1 1 0 30 38 20 0 0 2 3 1 0
Endosome 0 5 1 1 0 0 0 1 0 0 0 0 0 8 3 19 17 15 3 8 3 1 4 4
Golgi 0 0 0 1 1 0 0 0 0 0 0 0 0 0 1 0 1 1 0 2 0 1 1 1
Mitochondria 59 11 31 30 25 23 62 65 84 94 75 74 0 1 1 13 8 5 13 12 18 8 7 8
Nucleus 2 2 1 0 1 1 4 2 2 0 3 0 0 0 0 3 1 5 7 12 0 3 4 0
Nuclear Periphery 0 0 1 0 0 1 0 0 2 1 0 1 0 0 0 0 1 0 0 0 1 0 1 0
Nucleolus 0 0 1 0 0 0 1 1 1 0 2 0 0 1 1 0 0 1 0 0 0 0 1 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 1 1 4 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 1 0 1 0 0 2 3 1 2 0 1 0 10 7 5 6 5 3 5 6 5
Vac/Vac Membrane 9 40 2 2 1 1 0 0 3 0 1 0 10 12 2 36 71 49 22 20 17 10 12 17
Unique Cell Count 141 266 144 109 183 109 195 225 219 190 124 156 358 490 489 538 474 452 157 296 155 245 337 269
Labelled Cell Count 175 284 166 130 200 128 236 261 273 247 155 191 360 501 493 599 555 505 157 296 155 245 337 269


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.4 4.5 3.7 4.0 3.8 4.1 3.3 3.7 3.3 3.2 3.1 3.3 4.6 4.4 4.4 5.1 6.5 5.9 4.0 4.1 5.0
Std Deviation (1e-4) 0.4 1.1 1.2 2.3 1.8 2.3 1.7 1.9 1.8 2.1 1.5 2.3 0.9 1.0 1.2 1.2 1.7 1.4 1.0 1.4 1.5
Intensity Change (Log2) – – – 0.12 0.04 0.14 -0.18 -0.0 -0.17 -0.23 -0.26 -0.16 0.32 0.25 0.25 0.46 0.8 0.68 0.1 0.15 0.43


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 2.5 4.4 3.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.3137 3.2261 3.2041 2.1781 2.4253 2.942 1.5622 1.8526 2.0639 2.091 1.7731 1.7684 3.2676 2.6699 3.0008 2.8525 3.1732 3.0277
Actin 0.021 0.0038 0.0156 0.0033 0.0172 0.0035 0.0258 0.001 0.0285 0.0009 0.0134 0.0047 0.0235 0.0006 0.0004 0.0006 0.0164 0.002
Bud 0.003 0.0134 0.0076 0.0005 0.0014 0.0003 0.0003 0.0011 0.005 0.0001 0.0079 0.0001 0.0024 0.0063 0.0028 0.0042 0.0009 0.0033
Bud Neck 0.0009 0.0007 0.0016 0.0107 0.0005 0.0016 0.0144 0.0002 0.0008 0.0004 0.001 0.0003 0.0146 0.0003 0.0002 0.0004 0.0006 0.001
Bud Periphery 0.0031 0.0083 0.0085 0.0012 0.0014 0.0004 0.0003 0.0014 0.0145 0.0002 0.0075 0.0001 0.0016 0.0032 0.0011 0.0019 0.001 0.001
Bud Site 0.0055 0.0166 0.0105 0.0001 0.0008 0.0002 0.0031 0.0016 0.0037 0.0017 0.0175 0.0001 0.0037 0.0105 0.0019 0.0022 0.0006 0.0006
Cell Periphery 0.0012 0.0004 0.0008 0.0001 0.0001 0.0001 0.0002 0.0003 0.0008 0.0011 0.0003 0.0001 0.0003 0.0002 0.0001 0.0002 0.0001 0.0001
Cytoplasm 0.1167 0.2101 0.0855 0.1504 0.1845 0.2595 0.0654 0.0934 0.1055 0.2085 0.0627 0.1887 0.1787 0.2075 0.1553 0.131 0.1874 0.2946
Cytoplasmic Foci 0.0163 0.01 0.0282 0.0275 0.0265 0.0153 0.0143 0.0022 0.0307 0.0361 0.027 0.0123 0.0079 0.0065 0.014 0.029 0.0424 0.0183
Eisosomes 0.001 0.0001 0.0006 0.0001 0.0001 0.0002 0.0004 0.0009 0.0004 0.0002 0.0002 0.0001 0.0004 0.0001 0.0001 0.0001 0.0003 0.0008
Endoplasmic Reticulum 0.0045 0.0053 0.0054 0.0028 0.0047 0.0031 0.0027 0.0011 0.0039 0.0099 0.0017 0.0068 0.0044 0.0027 0.0013 0.002 0.0017 0.0018
Endosome 0.019 0.0065 0.006 0.0108 0.0218 0.0086 0.0165 0.0005 0.0157 0.0719 0.0314 0.0025 0.0061 0.0022 0.0014 0.0017 0.0108 0.0021
Golgi 0.0029 0.0011 0.0024 0.0019 0.0021 0.0005 0.0033 0.0001 0.0036 0.0069 0.0187 0.0005 0.0012 0.0001 0.0002 0.0002 0.0043 0.0005
Lipid Particles 0.0067 0.001 0.0026 0.0038 0.0042 0.0012 0.0038 0.0002 0.0042 0.0285 0.0161 0.0005 0.0025 0.0005 0.001 0.0006 0.0117 0.0021
Mitochondria 0.0044 0.0023 0.0047 0.0069 0.003 0.0008 0.0012 0.0002 0.0013 0.0026 0.0667 0.0003 0.0019 0.0006 0.0004 0.0007 0.0036 0.0034
None 0.7496 0.6628 0.7932 0.7621 0.6502 0.6369 0.8227 0.8916 0.7403 0.6132 0.6621 0.7644 0.664 0.7264 0.801 0.7676 0.6453 0.6371
Nuclear Periphery 0.0111 0.0058 0.0039 0.0019 0.021 0.0061 0.0036 0.0005 0.0092 0.002 0.0292 0.0015 0.0199 0.0022 0.001 0.002 0.0086 0.0015
Nucleolus 0.002 0.0018 0.0015 0.0008 0.0006 0.001 0.0022 0.0002 0.0009 0.0004 0.0014 0.0001 0.0011 0.0009 0.0007 0.0037 0.0022 0.0013
Nucleus 0.0096 0.0281 0.0046 0.0039 0.0082 0.0412 0.0049 0.0022 0.0105 0.0022 0.0163 0.0042 0.0435 0.0199 0.0059 0.0094 0.0043 0.0141
Peroxisomes 0.0024 0.0014 0.0081 0.0023 0.0218 0.0015 0.0017 0.0001 0.0036 0.0013 0.006 0.0103 0.0113 0.0003 0.0008 0.0003 0.0045 0.0009
Punctate Nuclear 0.0129 0.0182 0.0062 0.007 0.0195 0.0168 0.0113 0.0007 0.0095 0.0027 0.0033 0.0018 0.0085 0.0073 0.0096 0.0402 0.0516 0.0123
Vacuole 0.0047 0.0021 0.0019 0.0012 0.0074 0.001 0.0017 0.0005 0.0062 0.0075 0.0048 0.0005 0.0017 0.0012 0.0006 0.0016 0.0009 0.0008
Vacuole Periphery 0.0015 0.0004 0.0007 0.0005 0.003 0.0003 0.0005 0 0.0013 0.0014 0.005 0.0001 0.0007 0.0001 0.0001 0.0004 0.0007 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 11.8885 4.6496 5.9146 9.1788 8.6704 2.9705 7.3876 6.7993 7.3349 10.1524
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –
Standard name
Human Ortholog
Description Involved in nuclear migration, part of the dynein/dynactin pathway; targets dynein to microtubule tips, which is necessary for sliding of microtubules along bud cortex; serves at interface between dynein's ATPase site and its microtubule binding stalk, causing individual dynein motors to remain attached to microtubules for long periods; synthetic lethal with bni1; homolog of human LIS1, mutations in which cause the severe brain disorder lissencephaly
Localization
Cell Percentages nucleus (36%), cytoplasm (5%), mixed (53%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Pac1

Pac1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pac1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available