Standard name | |
---|---|
Human Ortholog | |
Description | Ribosomal assembly factor and 66S pre-ribosomal particle constituent; subunit of the Nop7-subcomplex (PeBoW complex), required for an early nucleolar step in pre-60S ribosomal particle maturation; interaction of its ubiquitin-like (UBL) domain with the MIDAS domain in the Rea1p tail triggers release of the subcomplex and possibly other biogenesis factors via cycles of ATP hydrolysis; involved in the processing of 27S pre-rRNA; contains an N-terminal UBL domain and seven C-terminal WD repeats |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 3 | 0 | 0 | 5 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 2 | 6 | 8 | 12 |
Bud Neck | 0 | 0 | 0 | 4 | 3 | 2 | 4 | 0 | 1 | 3 | 0 | 3 | 7 | 12 | 12 | 1 | 1 | 1 | 0 | 0 | 0 | 1 | 3 | 13 |
Bud Site | 0 | 0 | 0 | 1 | 3 | 7 | 8 | 9 | 7 | 16 | 6 | 18 | 0 | 2 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 8 | 5 | 0 | 4 | 2 | 1 | 5 | 7 | 12 | 8 | 2 | 5 | 29 | 66 | 118 | 10 | 4 | 7 | 0 | 2 | 0 | 0 | 1 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 1 |
Endosome | 9 | 2 | 0 | 2 | 27 | 8 | 34 | 41 | 3 | 12 | 6 | 6 | 26 | 43 | 69 | 6 | 2 | 1 | 7 | 1 | 1 | 15 | 24 | 34 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 6 | 4 |
Mitochondria | 25 | 11 | 14 | 4 | 12 | 43 | 36 | 55 | 50 | 80 | 35 | 76 | 14 | 10 | 3 | 22 | 14 | 8 | 2 | 0 | 0 | 4 | 5 | 7 |
Nucleus | 78 | 37 | 20 | 4 | 9 | 11 | 16 | 20 | 19 | 17 | 12 | 30 | 36 | 48 | 34 | 20 | 17 | 13 | 48 | 29 | 12 | 46 | 35 | 62 |
Nuclear Periphery | 2 | 1 | 0 | 0 | 0 | 1 | 1 | 4 | 1 | 2 | 1 | 2 | 0 | 0 | 0 | 2 | 2 | 0 | 8 | 0 | 1 | 6 | 1 | 5 |
Nucleolus | 318 | 125 | 54 | 162 | 219 | 164 | 263 | 330 | 194 | 240 | 142 | 238 | 659 | 879 | 960 | 171 | 157 | 111 | 297 | 113 | 60 | 409 | 438 | 503 |
Peroxisomes | 1 | 0 | 0 | 7 | 19 | 21 | 44 | 30 | 12 | 11 | 5 | 13 | 27 | 118 | 138 | 4 | 4 | 0 | 0 | 0 | 0 | 0 | 3 | 5 |
SpindlePole | 10 | 6 | 5 | 81 | 130 | 82 | 168 | 206 | 77 | 120 | 69 | 75 | 69 | 198 | 277 | 11 | 11 | 4 | 26 | 10 | 11 | 69 | 237 | 180 |
Vac/Vac Membrane | 5 | 3 | 1 | 0 | 2 | 2 | 5 | 2 | 1 | 2 | 1 | 2 | 2 | 3 | 4 | 5 | 2 | 2 | 11 | 6 | 3 | 14 | 23 | 39 |
Unique Cell Count | 387 | 161 | 71 | 177 | 252 | 201 | 340 | 421 | 252 | 307 | 174 | 306 | 711 | 1000 | 1158 | 209 | 182 | 133 | 414 | 171 | 93 | 586 | 806 | 894 |
Labelled Cell Count | 456 | 190 | 94 | 269 | 426 | 343 | 586 | 705 | 380 | 511 | 279 | 473 | 870 | 1380 | 1615 | 253 | 215 | 148 | 414 | 171 | 93 | 586 | 806 | 894 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 10.5 | 10.0 | 8.8 | 9.9 | 8.4 | 7.0 | 6.5 | 6.8 | 6.4 | 6.1 | 6.0 | 6.0 | 13.8 | 13.1 | 12.3 | 18.5 | 20.6 | 22.5 | 13.0 | 12.0 | 12.6 |
Std Deviation (1e-4) | 2.3 | 2.7 | 2.9 | 3.9 | 2.9 | 2.8 | 2.1 | 2.0 | 1.7 | 2.3 | 1.6 | 1.7 | 4.5 | 5.1 | 4.6 | 10.1 | 9.3 | 11.6 | 4.2 | 3.2 | 3.0 |
Intensity Change (Log2) | – | – | – | 0.17 | -0.07 | -0.32 | -0.43 | -0.38 | -0.45 | -0.53 | -0.55 | -0.55 | 0.65 | 0.57 | 0.49 | 1.08 | 1.23 | 1.36 | 0.57 | 0.45 | 0.52 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.8 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 2.9 | 0 | 2.8 | 2.7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | -4.8 | -4.1 | 0.3 | -2.1 | -1.5 | 0 | 1.1 | 0.1 | 0.9 | 0 | 0 | 0 | -2.0 | -2.7 | -3.0 |
Nucleus | -6.2 | -6.4 | -5.2 | -6.4 | -6.7 | -4.7 | -5.8 | -4.5 | -4.1 | -7.2 | 0 | 0 | -3.9 | -3.8 | -3.4 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 3.3 | 2.2 | 1.0 | 0.2 | 0.4 | 0.2 | 0.4 | 1.0 | 0.3 | 4.7 | 2.9 | 1.5 | 1.1 | 2.0 | 1.3 |
Peroxisomes | 0 | 0 | 2.8 | 3.2 | 0 | 0 | 0 | 0 | 0 | 0 | 3.1 | 3.1 | 0 | 0 | 0 |
SpindlePole | 5.8 | 6.7 | 5.2 | 6.6 | 6.6 | 4.0 | 5.2 | 5.0 | 3.2 | 0.7 | 2.7 | 3.3 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 11.3933 | 17.1182 | 14.3544 | 12.5455 | 12.2348 | 14.566 | 7.6923 | 10.8261 | 10.3592 | 8.1945 | 6.2627 | 9.9212 | 9.955 | 13.0466 | 11.6742 | 12.3749 | 12.1078 | 13.0425 |
Actin | 0.0016 | 0 | 0.0124 | 0 | 0.0005 | 0.0002 | 0.0011 | 0 | 0.0183 | 0 | 0.0035 | 0 | 0.0582 | 0 | 0.0013 | 0 | 0.0053 | 0.0034 |
Bud | 0.0003 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0.0002 | 0 | 0.0002 | 0.0003 | 0.0002 | 0.0004 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 |
Bud Neck | 0.0007 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0003 | 0.0002 | 0.0001 | 0.0007 | 0.0007 | 0.0003 | 0.0006 | 0.0002 | 0.0003 | 0.0004 | 0.0003 | 0.0002 |
Bud Periphery | 0.0006 | 0.0001 | 0.0002 | 0 | 0.0002 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0.0012 | 0.0004 | 0.0007 | 0.0002 | 0.0003 | 0.0003 | 0.0002 | 0.0002 |
Bud Site | 0.0025 | 0.0002 | 0.0005 | 0 | 0.0002 | 0.0001 | 0.0007 | 0.0005 | 0.0003 | 0.003 | 0.001 | 0.0001 | 0.0016 | 0.0002 | 0.0014 | 0.0002 | 0.0002 | 0.0002 |
Cell Periphery | 0.0007 | 0.0002 | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0002 | 0.0005 | 0.0001 | 0.0004 | 0.0006 | 0.0002 | 0.0002 | 0.0003 | 0.0002 | 0.0003 | 0.0001 | 0.0001 |
Cytoplasm | 0.0034 | 0 | 0.0009 | 0 | 0.0001 | 0.0001 | 0.0044 | 0.0013 | 0.0013 | 0.0017 | 0.0002 | 0.0001 | 0.0032 | 0.0092 | 0.0041 | 0 | 0.0024 | 0.0001 |
Cytoplasmic Foci | 0.0339 | 0.0005 | 0.0023 | 0 | 0.0153 | 0.0015 | 0.0269 | 0.0021 | 0.0022 | 0.0008 | 0.0065 | 0.0003 | 0.0168 | 0.0003 | 0.0065 | 0.0001 | 0.0009 | 0.0006 |
Eisosomes | 0.0002 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0004 | 0 | 0.0006 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0016 | 0 | 0.0046 | 0 | 0.0003 | 0.0001 | 0.0007 | 0.0001 | 0.0002 | 0.0003 | 0.001 | 0 | 0.0018 | 0.0002 | 0.0047 | 0 | 0.0082 | 0.0004 |
Endosome | 0.0111 | 0 | 0.0108 | 0 | 0.0022 | 0.0002 | 0.0108 | 0.0134 | 0.0025 | 0.0014 | 0.0526 | 0.0001 | 0.0166 | 0.0004 | 0.008 | 0.0001 | 0.0105 | 0.0012 |
Golgi | 0.0015 | 0 | 0.0024 | 0 | 0.0029 | 0.0002 | 0.0018 | 0.0001 | 0.0007 | 0.0001 | 0.0248 | 0.0001 | 0.0072 | 0 | 0.0011 | 0 | 0.0008 | 0.0004 |
Lipid Particles | 0.0218 | 0.0006 | 0.0052 | 0.0001 | 0.1031 | 0.0029 | 0.0255 | 0.0012 | 0.0017 | 0.0018 | 0.1549 | 0.0059 | 0.0358 | 0.0004 | 0.0015 | 0.0004 | 0.001 | 0.0097 |
Mitochondria | 0.0044 | 0.0001 | 0.0033 | 0 | 0.015 | 0.0007 | 0.002 | 0.0015 | 0.0006 | 0.0004 | 0.1011 | 0.0081 | 0.0064 | 0.0002 | 0.0026 | 0.0003 | 0.0018 | 0.0012 |
None | 0.0038 | 0 | 0.0006 | 0 | 0.0002 | 0.0002 | 0.0195 | 0.0003 | 0.0107 | 0.0044 | 0.0007 | 0.0001 | 0.0082 | 0 | 0.0045 | 0 | 0.0006 | 0.0003 |
Nuclear Periphery | 0.0108 | 0.003 | 0.0139 | 0 | 0.0076 | 0.0026 | 0.0116 | 0.0033 | 0.0009 | 0.0018 | 0.0074 | 0.0169 | 0.0163 | 0.0067 | 0.0188 | 0.0002 | 0.0261 | 0.0283 |
Nucleolus | 0.7198 | 0.8778 | 0.8454 | 0.8248 | 0.7505 | 0.8828 | 0.6612 | 0.6288 | 0.9059 | 0.775 | 0.4365 | 0.7277 | 0.6757 | 0.7297 | 0.8178 | 0.8238 | 0.795 | 0.8469 |
Nucleus | 0.1105 | 0.1081 | 0.0733 | 0.173 | 0.0689 | 0.0962 | 0.1395 | 0.2906 | 0.0464 | 0.1967 | 0.178 | 0.2166 | 0.0707 | 0.1972 | 0.1041 | 0.1706 | 0.1387 | 0.0969 |
Peroxisomes | 0.0017 | 0 | 0.0018 | 0 | 0.0016 | 0.0001 | 0.0036 | 0.0001 | 0.0017 | 0.0001 | 0.0082 | 0.0002 | 0.0195 | 0 | 0.0004 | 0 | 0.0002 | 0.0001 |
Punctate Nuclear | 0.0436 | 0.0077 | 0.0156 | 0.0018 | 0.0253 | 0.0113 | 0.0873 | 0.0219 | 0.0004 | 0.0069 | 0.006 | 0.0082 | 0.0568 | 0.0143 | 0.0131 | 0.0014 | 0.0054 | 0.007 |
Vacuole | 0.0129 | 0.0008 | 0.0036 | 0.0001 | 0.0014 | 0.0001 | 0.0011 | 0.0251 | 0.0046 | 0.0035 | 0.005 | 0.0012 | 0.0012 | 0.0249 | 0.0051 | 0.0013 | 0.0006 | 0.0003 |
Vacuole Periphery | 0.0127 | 0.0006 | 0.0027 | 0 | 0.0042 | 0.0004 | 0.0015 | 0.0088 | 0.0012 | 0.0004 | 0.0093 | 0.0131 | 0.0013 | 0.0155 | 0.0038 | 0.0005 | 0.0016 | 0.0024 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 84.5704 | 74.8876 | 61.3914 | 48.5979 | 52.2385 | 37.4774 | 47.5946 | 41.3707 | 33.155 | 58.9594 |
Translational Efficiency | 1.513 | 1.628 | 1.6323 | 1.6235 | 1.8808 | 2.3371 | 1.7899 | 1.2799 | 1.5608 | 1.474 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
141 | 1349 | 1518 | 1225 | 92 | 1848 | 2127 | 196 | 233 | 3197 | 3645 | 1421 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1015.65 | 1115.73 | 1528.56 | 1665.65 | 1138.66 | 1232.44 | 1595.61 | 1932.39 | 1064.22 | 1183.19 | 1567.69 | 1702.44 |
Standard Deviation | 185.84 | 307.51 | 325.14 | 496.47 | 193.96 | 257.26 | 356.42 | 443.03 | 198.42 | 285.45 | 345.32 | 498.02 |
Intensity Change Log 2 | — | 0.135585 | 0.589770 | 0.713682 | — | 0.114180 | 0.486771 | 0.763049 | — | 0.124311 | 0.536247 | 0.739985 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000736 | 0.000355 | 0.000567 | 0.000885 | 0.000230 | 0.000340 | 0.000622 | 0.000561 | 0.000536 | 0.000346 | 0.000599 | 0.000840 |
Bud Neck | 0.002361 | 0.002154 | 0.004800 | 0.010860 | 0.000821 | 0.001962 | 0.006991 | 0.012229 | 0.001753 | 0.002043 | 0.006078 | 0.011049 |
Bud Site | 0.000299 | 0.000808 | 0.000592 | 0.002286 | 0.000166 | 0.000288 | 0.000655 | 0.002582 | 0.000246 | 0.000507 | 0.000629 | 0.002327 |
Cell Periphery | 0.000026 | 0.000089 | 0.000032 | 0.000204 | 0.000010 | 0.000021 | 0.000067 | 0.000081 | 0.000019 | 0.000050 | 0.000053 | 0.000187 |
Cytoplasm | 0.000405 | 0.001154 | 0.000160 | 0.000865 | 0.000029 | 0.000089 | 0.000321 | 0.000694 | 0.000257 | 0.000539 | 0.000254 | 0.000842 |
Cytoplasmic Foci | 0.000663 | 0.002364 | 0.001190 | 0.004667 | 0.000357 | 0.001051 | 0.000582 | 0.009220 | 0.000542 | 0.001605 | 0.000836 | 0.005295 |
Eisosomes | 0.000058 | 0.000085 | 0.000067 | 0.000091 | 0.000065 | 0.000078 | 0.000068 | 0.000072 | 0.000061 | 0.000081 | 0.000067 | 0.000088 |
Endoplasmic Reticulum | 0.002678 | 0.000692 | 0.000378 | 0.000727 | 0.000532 | 0.000396 | 0.000366 | 0.000191 | 0.001830 | 0.000521 | 0.000371 | 0.000653 |
Endosome | 0.000353 | 0.000444 | 0.000073 | 0.001276 | 0.000095 | 0.000277 | 0.000144 | 0.000911 | 0.000251 | 0.000347 | 0.000115 | 0.001226 |
Golgi | 0.000454 | 0.000549 | 0.000331 | 0.001738 | 0.000376 | 0.000366 | 0.000263 | 0.001113 | 0.000423 | 0.000443 | 0.000291 | 0.001652 |
Lipid Particles | 0.000284 | 0.000392 | 0.000030 | 0.000209 | 0.000023 | 0.000474 | 0.000027 | 0.000117 | 0.000181 | 0.000439 | 0.000028 | 0.000197 |
Mitochondria | 0.005595 | 0.000873 | 0.002086 | 0.005315 | 0.000418 | 0.000792 | 0.002553 | 0.003390 | 0.003550 | 0.000826 | 0.002358 | 0.005049 |
Mitotic Spindle | 0.010857 | 0.003523 | 0.014536 | 0.020194 | 0.017248 | 0.005222 | 0.005678 | 0.027566 | 0.013380 | 0.004505 | 0.009367 | 0.021211 |
None | 0.000949 | 0.002334 | 0.000631 | 0.003132 | 0.000359 | 0.000779 | 0.001398 | 0.000937 | 0.000716 | 0.001435 | 0.001079 | 0.002829 |
Nuclear Periphery | 0.017574 | 0.000311 | 0.004827 | 0.003348 | 0.000195 | 0.000474 | 0.003386 | 0.001298 | 0.010712 | 0.000405 | 0.003987 | 0.003066 |
Nuclear Periphery Foci | 0.014927 | 0.002157 | 0.002314 | 0.002054 | 0.002095 | 0.001375 | 0.001262 | 0.000784 | 0.009860 | 0.001705 | 0.001700 | 0.001879 |
Nucleolus | 0.581915* | 0.666103* | 0.691947* | 0.592035* | 0.658932* | 0.664274* | 0.583601* | 0.666855* | 0.612325* | 0.665046* | 0.628723* | 0.602355* |
Nucleus | 0.346382* | 0.287423* | 0.240617* | 0.318013* | 0.293854* | 0.305312* | 0.375064* | 0.228879* | 0.325642* | 0.297764* | 0.319072* | 0.305718* |
Peroxisomes | 0.010555 | 0.026700 | 0.033115 | 0.017379 | 0.023745 | 0.015395 | 0.013472 | 0.033669 | 0.015763 | 0.020165 | 0.021652 | 0.019626 |
Vacuole | 0.001056 | 0.000840 | 0.000475 | 0.004980 | 0.000230 | 0.000466 | 0.000732 | 0.003285 | 0.000730 | 0.000624 | 0.000625 | 0.004746 |
Vacuole Periphery | 0.001874 | 0.000651 | 0.001231 | 0.009742 | 0.000222 | 0.000568 | 0.002748 | 0.005564 | 0.001221 | 0.000603 | 0.002116 | 0.009165 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 2.44 | 1.02 | -1.07 | -9.87 | -6.55 | -1.11 | -12.67 | -5.35 | -2.85 | -0.87 | 1.70 | -0.80 | -3.36 | -7.46 | -6.17 |
Bud Neck | 0.14 | -5.05 | -12.12 | -12.87 | -9.32 | -5.69 | -17.62 | -6.50 | -6.14 | -4.25 | -1.17 | -13.21 | -14.66 | -14.70 | -9.08 |
Bud Site | -2.64 | -4.36 | -9.07 | -5.47 | -7.59 | -2.45 | -9.91 | -3.39 | -3.31 | -3.04 | -2.94 | -8.44 | -9.09 | -7.87 | -7.64 |
Cell Periphery | -1.85 | -1.49 | -4.41 | -2.24 | -4.21 | -3.34 | -6.18 | -3.72 | -3.30 | -1.64 | -2.07 | -5.34 | -4.93 | -3.80 | -3.93 |
Cytoplasm | -1.22 | 0.64 | -1.52 | 0.53 | -6.84 | -2.47 | -6.87 | -4.16 | -3.83 | -2.41 | -1.00 | -0.13 | -2.93 | -1.14 | -6.32 |
Cytoplasmic Foci | -3.09 | -3.06 | -6.42 | -2.44 | -5.52 | -2.23 | -2.62 | -3.10 | -2.89 | -3.03 | -3.56 | -2.85 | -7.07 | -5.04 | -6.63 |
Eisosomes | -3.87 | -1.57 | -4.56 | -1.23 | -5.29 | -1.88 | -0.28 | -1.51 | 0.12 | -1.82 | -4.03 | -1.39 | -5.10 | -2.05 | -5.92 |
Endoplasmic Reticulum | 4.29 | 4.95 | 3.81 | -0.11 | -1.28 | 1.35 | 1.57 | 1.72 | 0.77 | 0.58 | 4.52 | 5.00 | 3.30 | -0.69 | -1.32 |
Endosome | -0.61 | 1.95 | -2.52 | -2.23 | -3.67 | -1.83 | -0.81 | -2.29 | -1.86 | -2.18 | -0.98 | 1.41 | -3.30 | -2.96 | -3.94 |
Golgi | -0.69 | 1.01 | -2.07 | -1.86 | -2.31 | 0.02 | 0.91 | -3.40 | -3.55 | -4.09 | -0.29 | 1.52 | -2.39 | -2.33 | -2.67 |
Lipid Particles | -0.48 | 2.69 | 0.71 | 0.83 | -3.72 | -1.35 | -1.13 | -2.59 | 0.38 | -2.54 | -1.18 | 2.63 | -0.65 | 0.98 | -4.44 |
Mitochondria | 1.36 | 0.99 | 0.04 | -12.93 | -9.31 | -2.67 | -14.76 | -7.77 | -6.67 | -2.59 | 1.29 | 0.52 | -0.79 | -14.23 | -8.95 |
Mitotic Spindle | 1.63 | -1.07 | -2.34 | -6.47 | -2.12 | 1.43 | 1.35 | -1.24 | -3.11 | -3.03 | 2.06 | 0.72 | -2.21 | -6.86 | -4.82 |
None | -2.09 | 0.70 | -3.16 | -0.95 | -4.57 | -1.25 | -2.20 | -5.07 | -0.79 | 0.70 | -1.87 | -1.01 | -4.02 | -2.50 | -3.18 |
Nuclear Periphery | 2.32 | 1.73 | 1.92 | -6.61 | 1.58 | -1.91 | -5.32 | -4.61 | -3.38 | 2.71 | 2.27 | 1.50 | 1.70 | -6.67 | 1.45 |
Nuclear Periphery Foci | 3.72 | 3.72 | 3.76 | 0.27 | 0.27 | 0.80 | 1.11 | 0.42 | -0.38 | -0.72 | 3.80 | 3.88 | 3.67 | -0.65 | -1.09 |
Nucleolus | -4.38 | -5.38 | 0.32 | 8.40 | 10.06 | -0.22 | 3.57 | 1.03 | 1.70 | -2.41 | -3.57 | -0.69 | 1.84 | 9.16 | 4.30 |
Nucleus | 3.42 | 5.96 | 1.12 | -3.82 | -8.00 | -0.52 | -3.70 | 2.01 | 3.78 | 7.69 | 2.02 | 0.25 | 0.76 | -1.93 | 0.89 |
Peroxisomes | -6.92 | -10.25 | -3.35 | 5.52 | 10.05 | 2.22 | 2.66 | -2.09 | -4.97 | -5.39 | -2.08 | -3.37 | -2.05 | -0.16 | 2.03 |
Vacuole | 0.67 | 2.09 | -5.08 | -5.52 | -6.02 | -2.59 | -6.88 | -3.11 | -3.01 | -2.87 | 0.51 | 0.30 | -6.31 | -6.53 | -6.49 |
Vacuole Periphery | 2.48 | 1.36 | -5.87 | -7.50 | -6.99 | -5.50 | -9.24 | -4.76 | -4.43 | -2.38 | 2.04 | -2.57 | -7.18 | -8.14 | -6.52 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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