Standard name
Human Ortholog
Description Phosphoprotein of the mRNA cap-binding complex; involved in translational control; repressor of cap-dependent translation initiation; competes with eIF4G for binding to eIF4E
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0.08 0.09 0.16 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.99 0.97 0.96 0.97 0.94 – 0.86 0.77 0.82 0.66 0.73 0.98 0.98 0.97 0.98 0.94 0.99 0.97 0.95 0.95 0.95 0.93 0.94
Endoplasmic Reticulum 0 0 0 0 0 0 – 0.06 0.1 0.06 0.11 0.12 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0.11 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 – 0.06 0.12 0.09 0.07 0.21 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 – 3 2 2 1 3 0 0 0 0 0 0 0 0 0 0 1 0
Bud 0 0 1 0 0 0 – 0 0 0 0 0 1 0 0 1 0 0 5 1 0 0 2 4
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 5 0 0 3 1 – 3 8 6 13 6 11 5 7 7 1 4 0 0 0 0 0 0
Cytoplasm 327 236 116 98 134 81 – 96 80 53 53 71 396 406 506 225 184 153 320 226 114 243 337 320
Endoplasmic Reticulum 1 0 1 2 4 3 – 7 10 4 9 12 11 6 14 5 8 6 0 3 2 3 10 4
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Golgi 1 0 0 0 0 0 – 0 1 1 9 1 0 2 1 1 0 0 0 2 0 0 1 1
Mitochondria 0 2 1 1 1 2 – 7 13 6 6 20 1 0 0 0 3 0 1 0 1 0 0 0
Nucleus 0 0 0 0 0 0 – 1 0 0 0 0 0 0 0 0 2 0 0 0 0 0 1 0
Nuclear Periphery 0 0 1 0 0 0 – 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 2 0 0 – 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 1 0 0 0 – 1 1 1 2 0 2 0 0 2 11 0 1 0 0 3 4 2
Unique Cell Count 329 238 120 102 138 86 112 104 65 80 97 406 413 521 230 196 155 331 237 120 257 363 342
Labelled Cell Count 331 243 121 103 142 88 118 115 73 93 114 422 419 528 241 210 163 331 237 120 257 363 342


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 38.4 50.5 26.2 34.2 33.6 27.1 – 23.0 21.7 21.5 22.9 19.6 43.5 43.2 39.7 56.3 47.1 54.3 41.2 42.8 42.6
Std Deviation (1e-4) 8.2 10.5 8.7 9.0 8.4 6.4 – 5.0 5.3 5.2 8.7 4.6 10.2 7.9 7.7 19.9 19.8 18.1 9.3 10.5 12.3
Intensity Change (Log2) – – – 0.38 0.36 0.05 – -0.19 -0.27 -0.29 -0.2 -0.42 0.73 0.72 0.6 1.1 0.85 1.05 0.65 0.71 0.7


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 4.6 0 0 0 0 0 0 0
Cytoplasm -0.2 0.2 -0.9 – -3.0 -4.5 -3.5 -5.8 -5.0 0.5 1.1 0.3 0.7 -1.1 1.2
Endoplasmic Reticulum 0 0 0 – 0 3.0 0 0 3.6 0 0 0 0 0 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 – 0 3.6 0 0 4.9 0 0 0 0 0 0
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 27.2441 36.8207 33.3774 31.6249 26.9605 33.0801 25.1056 36.4658 32.0864 30.3901 29.2295 31.3973 26.6157 39.1891 33.947 33.3323 30.9464 36.0099
Actin 0.0187 0.0002 0.0061 0.0003 0.0073 0.0007 0.0197 0.0004 0.0111 0.0188 0.003 0.0014 0.0333 0.0002 0.0042 0.0551 0.0019 0.0015
Bud 0.0009 0.0002 0.0007 0.0006 0.0013 0.0004 0.0017 0.0002 0.0011 0.0016 0.0006 0.0005 0.0007 0.0002 0.0008 0.0005 0.0003 0.0005
Bud Neck 0.0014 0.0002 0.0006 0.0002 0.0008 0.0009 0.0012 0.0003 0.0005 0.0035 0.0005 0.0015 0.0026 0.0001 0.0004 0.0046 0.0003 0.0018
Bud Periphery 0.0009 0 0.0004 0.0003 0.0016 0.0002 0.002 0.0001 0.0006 0.004 0.0003 0.0003 0.0006 0 0.0005 0.0003 0.0003 0.0001
Bud Site 0.0017 0.0002 0.0005 0.0002 0.0028 0.0001 0.0029 0.0002 0.0013 0.0081 0.0007 0.0002 0.0028 0.0002 0.0006 0.0029 0.0002 0
Cell Periphery 0.0016 0.0005 0.0006 0.0003 0.0013 0.0004 0.0024 0.0012 0.0005 0.0011 0.0002 0.0003 0.0008 0.0002 0.0004 0.0002 0.0002 0.0001
Cytoplasm 0.7917 0.9834 0.9061 0.9407 0.7516 0.9024 0.7304 0.9815 0.9148 0.862 0.8637 0.8896 0.6967 0.99 0.9014 0.8341 0.8969 0.9278
Cytoplasmic Foci 0.0102 0.0014 0.0046 0.0034 0.0406 0.0068 0.0212 0.0018 0.0137 0.0036 0.0294 0.0078 0.0491 0.0014 0.0067 0.023 0.0174 0.008
Eisosomes 0.0002 0 0.0001 0 0.0009 0 0.0002 0 0 0.0008 0.0002 0 0.0002 0 0.0001 0.0002 0.0001 0
Endoplasmic Reticulum 0.0537 0.0057 0.0213 0.0018 0.0209 0.0362 0.0601 0.0055 0.0069 0.0032 0.0023 0.0535 0.0366 0.0016 0.0199 0.0055 0.0134 0.0342
Endosome 0.0209 0.002 0.0151 0.0087 0.0265 0.0281 0.0374 0.0018 0.0126 0.0164 0.0173 0.0177 0.0226 0.0015 0.0169 0.0174 0.0336 0.0161
Golgi 0.0082 0.0003 0.0019 0.0004 0.0082 0.0044 0.0118 0.0003 0.0033 0.0063 0.0048 0.0059 0.007 0.0002 0.0067 0.0041 0.0023 0.0022
Lipid Particles 0.0123 0.0002 0.0036 0.0001 0.0143 0.0001 0.0101 0.0002 0.0011 0.0067 0.0134 0.0039 0.0231 0 0.0011 0.003 0.0062 0.0001
Mitochondria 0.0209 0.0001 0.0022 0.0002 0.0809 0.0089 0.0084 0.0001 0.0036 0.0289 0.0089 0.0043 0.0049 0 0.0027 0.0005 0.0063 0.0017
None 0.0022 0.0001 0.0005 0.0001 0.0099 0.0003 0.0106 0.0001 0.001 0.0007 0.0144 0.0003 0.0046 0.0001 0.0049 0.0022 0.0061 0.0003
Nuclear Periphery 0.0108 0.0006 0.0034 0.001 0.0029 0.0015 0.0133 0.0008 0.0017 0.0023 0.0037 0.0016 0.0113 0.0005 0.004 0.0037 0.0037 0.0014
Nucleolus 0.0004 0 0.0001 0 0.0014 0 0.0017 0 0.0001 0.003 0.0048 0 0.0012 0 0.0004 0.0002 0.0002 0
Nucleus 0.0032 0.0006 0.0013 0.001 0.0031 0.0008 0.0119 0.0007 0.0015 0.003 0.0048 0.0009 0.0071 0.0005 0.0024 0.0018 0.0012 0.0009
Peroxisomes 0.0047 0 0.001 0 0.0041 0.0001 0.0031 0 0.0033 0.0016 0.0081 0.0036 0.0342 0 0.0002 0.0035 0.0004 0.0001
Punctate Nuclear 0.0017 0 0.0003 0 0.0037 0 0.0047 0 0.0011 0.0004 0.0083 0.0003 0.0156 0 0.0009 0.0049 0.0006 0.0001
Vacuole 0.0285 0.0039 0.0253 0.0345 0.01 0.0049 0.0375 0.0045 0.017 0.0175 0.0065 0.0048 0.0362 0.0028 0.018 0.0284 0.0057 0.0021
Vacuole Periphery 0.0053 0.0004 0.0043 0.006 0.0059 0.003 0.0077 0.0003 0.0033 0.0065 0.0042 0.0016 0.009 0.0003 0.0069 0.0041 0.0025 0.001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 255.4177 192.8102 197.2908 250.5815 256.0972 240.527 225.2187 227.8381 206.7712 236.7019
Translational Efficiency 2.1837 2.6619 2.6159 1.9083 2.2547 2.4028 2.2125 1.8768 2.3005 2.2822

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1651 1368 1072 1239 150 2101 1949 1479 1801 3469 3021 2718

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 2510.35 2574.00 3863.43 3843.69 2411.92 2597.66 3492.31 3658.16 2502.15 2588.33 3624.00 3742.73
Standard Deviation 478.86 542.12 589.94 738.31 439.15 511.54 591.37 755.51 476.45 523.94 616.97 753.40
Intensity Change Log 2 — 0.036124 0.621994 0.614603 — 0.107031 0.534000 0.600936 — 0.071304 0.579547 0.607923

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000023 0.000139 0.000176 0.000458 0.000335 0.000181 0.000083 0.000294 0.000049 0.000164 0.000116 0.000369
Bud Neck 0.000335 0.000445 0.003314 0.001500 0.001428 0.000309 0.000965 0.001184 0.000426 0.000363 0.001799 0.001328
Bud Site 0.004550 0.008410 0.003746 0.011272 0.009654 0.005265 0.003490 0.011993 0.004975 0.006505 0.003581 0.011664
Cell Periphery 0.000394 0.002728 0.006981 0.036373 0.014808 0.000981 0.002695 0.019508 0.001594 0.001670 0.004216 0.027196
Cytoplasm 0.960278* 0.887121* 0.729742* 0.676622* 0.691895* 0.922781* 0.818497* 0.778594* 0.937925* 0.908718* 0.787003* 0.732110*
Cytoplasmic Foci 0.008907 0.015069 0.016848 0.004965 0.028849 0.012845 0.006171 0.004450 0.010568 0.013722 0.009960 0.004685
Eisosomes 0.000001 0.000001 0.000003 0.000007 0.000003 0.000001 0.000001 0.000004 0.000001 0.000001 0.000002 0.000005
Endoplasmic Reticulum 0.000303 0.001178 0.000380 0.000576 0.001520 0.000574 0.000275 0.000424 0.000404 0.000812 0.000312 0.000494
Endosome 0.000171 0.001809 0.001297 0.001516 0.005137 0.001300 0.001072 0.000789 0.000585 0.001501 0.001152 0.001120
Golgi 0.002293 0.036042 0.037438 0.019269 0.115004* 0.021318 0.017220 0.013606 0.011681 0.027125 0.024394 0.016187
Lipid Particles 0.000275 0.004163 0.011376 0.028404 0.020486 0.002314 0.006014 0.017670 0.001958 0.003043 0.007917 0.022563
Mitochondria 0.000448 0.000395 0.000144 0.000491 0.000055 0.000201 0.000181 0.000203 0.000415 0.000277 0.000168 0.000334
Mitotic Spindle 0.000081 0.002890 0.004633 0.000601 0.001602 0.001419 0.001169 0.000447 0.000208 0.001999 0.002398 0.000517
None 0.006837 0.001124 0.001625 0.002427 0.000429 0.000566 0.000572 0.000995 0.006304 0.000786 0.000946 0.001648
Nuclear Periphery 0.000010 0.000099 0.001420 0.000303 0.000172 0.000042 0.000217 0.000144 0.000023 0.000064 0.000644 0.000217
Nuclear Periphery Foci 0.000143 0.006433 0.019070 0.005981 0.015537 0.003096 0.005829 0.003947 0.001426 0.004412 0.010527 0.004874
Nucleolus 0.000048 0.000035 0.000097 0.000132 0.000034 0.000023 0.000081 0.000121 0.000047 0.000028 0.000087 0.000126
Nucleus 0.002777 0.003916 0.024765 0.010426 0.003448 0.002602 0.010536 0.007680 0.002832 0.003120 0.015585 0.008932
Peroxisomes 0.000078 0.000037 0.000014 0.000129 0.000033 0.000045 0.000025 0.000146 0.000074 0.000042 0.000021 0.000138
Vacuole 0.005773 0.013923 0.106134 0.179570 0.047438 0.012854 0.103421 0.123378 0.009244 0.013275 0.104383 0.148993
Vacuole Periphery 0.006275 0.014043 0.030798 0.018979 0.042133 0.011282 0.021486 0.014424 0.009261 0.012371 0.024791 0.016500

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -9.78 -13.77 -3.75 -2.72 -2.13 1.72 3.52 0.28 -1.73 -4.46 -3.38 -9.77 -5.55 -3.10 -4.06
Bud Neck -0.74 -10.96 -6.61 -8.96 7.22 4.27 0.35 0.42 -7.45 0.12 0.29 -9.47 -6.08 -11.62 5.15
Bud Site -4.10 0.52 -5.95 -2.08 -6.56 2.92 3.93 -1.41 -5.51 -6.84 -2.62 1.73 -7.55 -5.72 -9.44
Cell Periphery -8.73 -13.50 -17.11 -15.85 -13.26 3.56 3.10 -1.01 -14.90 -13.27 -0.23 -6.68 -20.72 -21.46 -18.80
Cytoplasm 12.45 26.02 30.67 20.19 2.54 -8.87 -4.16 -3.03 19.18 3.18 6.51 27.23 31.55 28.06 5.31
Cytoplasmic Foci -5.48 -7.62 4.68 9.36 10.49 3.69 5.10 5.65 10.21 4.20 -3.64 -1.31 7.54 13.78 10.70
Eisosomes -0.21 -6.47 -5.05 -5.10 -2.90 3.00 3.55 -0.93 -4.28 -5.22 0.27 -2.97 -6.30 -6.50 -5.31
Endoplasmic Reticulum -3.25 -1.05 -2.32 2.23 -1.79 2.70 3.75 3.20 1.10 -1.40 -2.98 1.32 -0.85 2.38 -2.38
Endosome -7.04 -14.00 -2.59 0.69 0.01 5.07 5.18 5.75 2.64 1.27 -6.63 -4.02 -1.99 1.61 0.56
Golgi -14.27 -21.01 -11.49 6.31 8.79 7.65 8.01 8.33 4.77 2.94 -8.80 -8.62 -2.69 7.56 7.41
Lipid Particles -9.97 -14.99 -15.20 -12.81 -7.72 3.98 3.07 0.58 -12.01 -8.41 -2.49 -11.46 -17.74 -17.58 -11.89
Mitochondria 0.22 9.33 -0.12 -0.26 -1.65 -1.94 -1.83 -2.91 0.01 -0.27 1.28 4.91 0.99 -0.34 -1.54
Mitotic Spindle -5.01 -8.63 -4.33 3.88 7.50 0.26 0.42 3.05 3.72 3.02 -6.82 -8.98 -3.50 5.29 7.46
None 3.64 3.33 2.44 -1.17 -0.68 -1.84 -2.06 -2.10 -1.62 -1.53 3.84 3.73 3.12 -1.67 -1.32
Nuclear Periphery -7.00 -13.43 -16.85 -10.26 10.80 4.68 -2.60 0.65 -9.66 5.31 -5.50 -15.68 -18.02 -14.37 11.06
Nuclear Periphery Foci -8.78 -17.31 -15.41 0.51 11.52 7.12 5.30 6.55 -2.25 4.70 -7.99 -19.06 -11.84 -1.29 11.45
Nucleolus 1.21 -9.60 -7.98 -7.17 -2.09 2.09 -8.80 -11.51 -15.83 -4.02 3.48 -10.24 -12.00 -14.54 -4.40
Nucleus -2.63 -16.47 -9.14 -7.48 8.77 1.21 -9.31 -6.02 -10.10 4.29 -1.22 -19.37 -12.01 -12.08 8.57
Peroxisomes 5.17 9.46 -2.84 -5.38 -6.92 -1.17 0.96 -4.36 -3.65 -4.67 3.69 8.02 -3.74 -5.65 -7.32
Vacuole -6.52 -20.39 -28.29 -26.62 -7.90 4.88 -8.24 -9.53 -23.52 -2.10 -3.87 -30.82 -36.07 -35.22 -7.32
Vacuole Periphery -5.45 -11.17 -9.15 -2.49 5.12 3.76 2.58 3.44 -2.07 4.61 -2.61 -10.49 -5.62 -3.45 6.45
Standard name
Human Ortholog
Description Phosphoprotein of the mRNA cap-binding complex; involved in translational control; repressor of cap-dependent translation initiation; competes with eIF4G for binding to eIF4E
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-2

Caf20

Caf20


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Caf20-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available