Standard name
Human Ortholog
Description Cytoplasmic protein of unknown function; computational analysis of large-scale protein-protein interaction data suggests a possible role in actin patch assembly
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0.07 0.13 0.1 0.11 0.13 0.15 0.2 0.16 0.16 0.17 0.06 0.05 0.05 0 0 0.05 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0.07 0 0.08 0 0.07 0.05 0 0.1 0.08 0.1 0.08 0 0 0 0 0.06 0.15 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.05 0.06 0.08 0.09 0.09 0.07 0.07 0.06 0.05 0.06 0 0.06 0.07 0.09 0.18 0.08 0.12 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.17 0.11 0.05 0.07 0.09 0.09 0 0 0 0 0 0 0.16 0.18 0.21 0.19 0.25 0.15 0.16 0.22 0.18 0.22 0.25 0.18
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.35 0.55 0.07 0.1 0 0.16 0.12 0.07 0.21 0.14 0.25 0.2 0.09 0.06 0 0.09 0.08 0.08 0.05 0.08 0 0.06 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.07 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.32 0.29 0.18 0.19 0.15 0.25 0.15 0.11 0.18 0.17 0.15 0.13 0.32 0.44 0.41 0.54 0.48 0.49 0.41 0.27 0.39 0.33 0.31 0.39
SpindlePole 0.41 0.21 0.78 0.71 0.77 0.61 0.73 0.81 0.63 0.68 0.64 0.72 0.66 0.63 0.63 0.52 0.69 0.64 0.29 0.24 0.32 0.3 0.28 0.3
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 5 4 5 7 5 7 2 2 5 12 7 3 9 1 0 0 1 1 1 0 1 7 7
Bud 4 2 16 30 34 29 59 53 63 67 80 91 11 24 26 2 2 5 2 2 4 4 3 7
Bud Neck 2 1 7 7 4 13 14 11 7 17 5 10 2 5 7 0 1 0 0 0 1 1 9 6
Bud Site 3 5 6 18 9 19 24 16 32 31 52 42 5 9 5 1 3 14 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0
Cytoplasm 4 4 14 17 28 24 34 26 20 22 30 23 12 34 47 14 4 11 0 2 1 4 7 2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Endosome 16 8 10 15 28 25 13 9 2 4 10 1 30 90 112 15 12 14 18 20 44 42 94 73
Golgi 0 0 0 1 1 0 0 0 0 0 0 0 0 2 0 0 0 0 2 2 1 0 2 5
Mitochondria 32 41 15 22 10 45 56 27 66 59 130 108 17 29 16 7 4 7 5 7 10 11 15 11
Nucleus 0 1 0 1 5 1 5 5 7 7 10 5 0 1 2 0 0 1 0 2 0 0 2 0
Nuclear Periphery 0 2 0 0 0 0 1 0 1 1 1 2 1 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 6 3 1 4 2 5 9 10 13 10 30 16 1 5 5 1 1 2 0 0 0 2 4 2
Peroxisomes 29 22 40 42 50 70 68 38 56 69 77 72 62 214 214 43 23 45 47 25 94 64 118 161
SpindlePole 38 16 169 158 254 169 340 291 195 279 325 390 127 306 332 41 33 59 33 21 79 58 106 125
Vac/Vac Membrane 0 2 1 1 2 2 2 1 0 4 0 0 0 5 3 1 1 2 0 2 0 1 3 3
Unique Cell Count 92 75 217 223 328 276 468 361 309 408 510 541 192 489 527 79 48 92 116 92 244 197 382 415
Labelled Cell Count 136 112 283 321 434 407 632 489 464 575 763 767 271 734 770 125 84 161 116 92 244 197 382 415


Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.7 4.4 5.3 5.2 5.8 5.0 4.9 5.2 4.4 4.5 4.3 4.4 5.2 5.6 5.2 6.2 7.1 7.4 5.7 5.8 6.0
Std Deviation (1e-4) 0.7 1.7 1.0 1.6 1.2 1.5 1.4 1.4 1.8 1.3 1.2 1.1 1.1 1.2 1.0 2.4 2.3 2.6 1.2 1.5 1.5
Intensity Change (Log2) – – – -0.03 0.13 -0.08 -0.11 -0.02 -0.26 -0.23 -0.28 -0.26 -0.02 0.07 -0.02 0.23 0.41 0.49 0.09 0.13 0.18


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.0119 0.95 0.846 1.0498 0.3618 0.6813 2.6797 3.2615 2.7386 2.1223 2.2728 2.5205 0.5675 0.6857 0.5835 0.5055 0.6658 0.5262
Actin 0.0084 0.0044 0.0013 0.0005 0.0007 0.0006 0.0018 0.0043 0.0165 0.0004 0.0002 0.0102 0.0118 0.0193 0.0108 0.0019 0.0002 0.0009
Bud 0.0001 0.0003 0 0 0 0 0.0001 0.0015 0.0015 0 0 0.0002 0.0008 0.0018 0.0002 0.0002 0 0
Bud Neck 0.0004 0.0031 0.0005 0.0168 0 0.0003 0.0003 0.0093 0.0004 0.0012 0 0.0011 0.001 0.0014 0.001 0.0003 0.0001 0.0004
Bud Periphery 0.0001 0.0003 0 0 0 0 0.0002 0.0024 0.0019 0 0 0.0003 0.0007 0.0018 0.0003 0.0002 0.0001 0
Bud Site 0.0005 0.0125 0.0004 0.0001 0.0001 0.0001 0.0005 0.0592 0.0083 0.0001 0 0.0006 0.0068 0.0128 0.0018 0.0008 0.0002 0.0001
Cell Periphery 0 0 0 0 0 0 0.0001 0.0004 0.0001 0 0 0.0001 0.0001 0.0007 0.0001 0 0.0001 0
Cytoplasm 0.0012 0.0005 0.0001 0 0.0001 0.0001 0.0056 0.0016 0.0041 0.0004 0.0001 0.0009 0.0006 0.0005 0.0003 0.0004 0.0001 0.0001
Cytoplasmic Foci 0.3828 0.4192 0.4141 0.3802 0.4281 0.3775 0.3362 0.32 0.3681 0.6425 0.4111 0.3944 0.3466 0.3627 0.3642 0.4638 0.291 0.3735
Eisosomes 0.0001 0 0 0 0 0 0.0009 0.0001 0.0001 0 0 0.0002 0.0001 0.0001 0.0001 0 0 0
Endoplasmic Reticulum 0.001 0.0001 0 0 0 0 0.0042 0.0001 0.0007 0 0 0.0003 0.0003 0.0001 0.0002 0.0007 0.0001 0
Endosome 0.0149 0.018 0.0107 0.0006 0.0026 0.0015 0.0273 0.0155 0.0222 0.0111 0.0023 0.0061 0.0092 0.0067 0.0102 0.0095 0.0395 0.0038
Golgi 0.0112 0.0107 0.0071 0.0033 0.006 0.0045 0.0057 0.0101 0.0094 0.0099 0.0044 0.0053 0.0105 0.0125 0.01 0.0069 0.0109 0.0071
Lipid Particles 0.1799 0.1105 0.1171 0.0722 0.147 0.1368 0.1077 0.1224 0.1239 0.0347 0.2595 0.1554 0.1633 0.1427 0.1655 0.1654 0.2977 0.1789
Mitochondria 0.0017 0.0012 0.002 0.0001 0.0003 0.0002 0.0021 0.0512 0.0025 0.0009 0.0007 0.0038 0.0021 0.0016 0.0016 0.0138 0.0031 0.0009
None 0.0028 0.0002 0.0001 0 0.0002 0.0001 0.0732 0.0357 0.0113 0.0005 0.0002 0.0086 0.0131 0.0015 0.0003 0.0009 0.0001 0.0001
Nuclear Periphery 0.0013 0.0005 0.0003 0 0.0001 0.0001 0.0383 0.0001 0.009 0 0 0.0019 0.0003 0.0002 0.0003 0.0005 0.0003 0.0001
Nucleolus 0.0022 0.0014 0.0005 0.0005 0.0001 0.0007 0.0018 0.0422 0.0013 0.0002 0 0.0033 0.0059 0.0123 0.0008 0.0004 0.0005 0.0005
Nucleus 0.0004 0.0004 0.0001 0.0001 0 0.0001 0.0139 0.002 0.0148 0 0 0.0014 0.0004 0.0006 0.0001 0.0002 0.0002 0.0001
Peroxisomes 0.2326 0.3367 0.3525 0.2337 0.3874 0.2355 0.2705 0.1983 0.3259 0.2807 0.2981 0.2339 0.2654 0.3165 0.3347 0.156 0.2916 0.3112
Punctate Nuclear 0.1579 0.0793 0.0925 0.2917 0.0271 0.2419 0.1029 0.1213 0.077 0.0172 0.0235 0.1696 0.1602 0.103 0.0972 0.1768 0.0483 0.1222
Vacuole 0.0002 0.0005 0.0004 0 0 0 0.0039 0.0021 0.0006 0.0001 0 0.0019 0.0005 0.0011 0.0002 0.0006 0.0109 0
Vacuole Periphery 0.0002 0.0001 0.0001 0 0 0 0.0029 0.0002 0.0006 0 0 0.0005 0.0002 0.0001 0.0001 0.0007 0.0047 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 11.3054 8.0851 0.9999 7.1775 5.8632 9.0393 4.428 3.6783 6.0001 11.8607
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2139 1080 1612 1234 1498 2042 1182 1307 3637 3122 2794 2541

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 657.42 781.79 829.72 879.74 662.45 780.46 833.42 830.00 659.49 780.92 831.29 854.16
Standard Deviation 79.41 104.05 98.43 119.36 86.35 107.54 107.78 108.00 82.38 106.35 102.51 116.34
Intensity Change Log 2 — 0.249966 0.335809 0.420262 — 0.236513 0.331232 0.325300 — 0.243230 0.333514 0.373381

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001393 0.008429 0.020679 0.027198 0.002356 0.001777 0.006055 0.019661 0.001789 0.004078 0.014492 0.023321
Bud Neck 0.013216 0.003744 0.002186 0.005022 0.004082 0.002257 0.015878 0.004875 0.009454 0.002771 0.007978 0.004947
Bud Site 0.013685 0.013681 0.008258 0.060689 0.012045 0.005821 0.017960 0.022875 0.013009 0.008540 0.012362 0.041239
Cell Periphery 0.000202 0.000141 0.000085 0.000141 0.000186 0.000076 0.000567 0.000123 0.000195 0.000098 0.000289 0.000131
Cytoplasm 0.019277 0.010344 0.002735 0.024288 0.014582 0.007012 0.025174 0.012406 0.017343 0.008164 0.012228 0.018176
Cytoplasmic Foci 0.473852* 0.254233* 0.169748 0.208313* 0.334993* 0.273362* 0.250090* 0.184770* 0.416659* 0.266744* 0.203737* 0.196203*
Eisosomes 0.000119 0.000118 0.000115 0.000090 0.000099 0.000078 0.000273 0.000094 0.000110 0.000092 0.000182 0.000092
Endoplasmic Reticulum 0.000162 0.000420 0.000404 0.000371 0.000642 0.000119 0.000639 0.000233 0.000360 0.000223 0.000504 0.000300
Endosome 0.024767 0.041438 0.034658 0.022351 0.030452 0.026966 0.048198 0.043977 0.027108 0.031972 0.040386 0.033474
Golgi 0.045819 0.045947 0.020434 0.014758 0.044129 0.042048 0.019393 0.021484 0.045123 0.043397 0.019994 0.018218
Lipid Particles 0.049314 0.051548 0.138735* 0.023254 0.059733 0.051402 0.127063* 0.083091 0.053605 0.051452 0.133797* 0.054032
Mitochondria 0.013892 0.003832 0.001337 0.002818 0.005286 0.004572 0.003422 0.001250 0.010347 0.004316 0.002219 0.002011
Mitotic Spindle 0.007579 0.022742 0.210993* 0.126048* 0.020863 0.019146 0.114561* 0.125662* 0.013051 0.020390 0.170197* 0.125849*
None 0.001240 0.000170 0.000049 0.000126 0.002653 0.000691 0.005003 0.000538 0.001822 0.000511 0.002144 0.000338
Nuclear Periphery 0.000038 0.000041 0.000204 0.000166 0.000061 0.000025 0.000356 0.000111 0.000048 0.000030 0.000268 0.000137
Nuclear Periphery Foci 0.002234 0.002917 0.005545 0.001934 0.006861 0.001992 0.016202 0.004867 0.004140 0.002312 0.010053 0.003442
Nucleolus 0.001199 0.001015 0.000192 0.000215 0.001646 0.000628 0.004757 0.000950 0.001383 0.000762 0.002123 0.000593
Nucleus 0.002360 0.001205 0.000235 0.000465 0.002097 0.000741 0.014089 0.000678 0.002252 0.000902 0.006096 0.000574
Peroxisomes 0.327832* 0.536058* 0.381601* 0.474078* 0.455505* 0.560084* 0.324785* 0.469694* 0.380418* 0.551773* 0.357565* 0.471823*
Vacuole 0.001455 0.001570 0.001694 0.006331 0.001541 0.000646 0.005300 0.002543 0.001490 0.000966 0.003220 0.004382
Vacuole Periphery 0.000365 0.000406 0.000114 0.001344 0.000188 0.000557 0.000236 0.000120 0.000292 0.000505 0.000166 0.000714

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.76 -10.57 -9.74 -6.15 -2.07 1.77 -4.40 -8.11 -8.42 -6.22 -4.20 -11.31 -12.73 -10.84 -4.43
Bud Neck 5.60 7.17 5.07 -1.52 -4.29 2.66 -5.46 -0.98 -2.79 4.64 6.82 1.34 4.09 -3.56 2.47
Bud Site -0.17 2.69 -12.22 -11.70 -13.57 4.01 -2.64 -4.59 -7.65 -2.00 3.42 0.04 -12.50 -14.52 -12.44
Cell Periphery 1.99 4.82 1.74 -0.61 -4.72 4.96 -4.15 2.65 -3.48 5.12 5.51 -2.14 3.13 -3.50 3.89
Cytoplasm 4.06 10.43 -3.24 -6.15 -9.97 4.46 -3.37 0.76 -3.34 3.88 6.78 3.15 -1.80 -7.33 -4.39
Cytoplasmic Foci 23.29 39.24 30.21 5.06 -5.06 6.84 8.30 16.84 11.17 7.31 22.75 33.57 34.75 11.76 1.30
Eisosomes -0.14 0.03 2.66 1.64 3.03 1.71 -4.74 0.88 -1.16 5.15 1.60 -4.29 2.62 0.26 5.84
Endoplasmic Reticulum -1.50 -5.60 -3.90 0.20 0.45 1.57 -0.17 1.22 -4.37 5.35 0.87 -1.23 0.33 -1.28 4.82
Endosome -5.62 -4.08 1.04 6.05 4.64 1.24 -4.90 -4.34 -5.70 0.69 -2.90 -6.39 -3.38 -0.71 2.66
Golgi -0.24 12.32 14.18 11.40 3.83 0.67 11.92 9.74 9.25 -1.43 0.63 17.01 16.71 14.78 1.58
Lipid Particles -0.34 -14.47 8.39 6.76 20.36 1.53 -8.53 -3.18 -4.80 5.49 0.57 -16.45 0.70 0.17 16.17
Mitochondria 5.79 8.29 6.90 0.99 -1.57 0.54 1.66 4.06 3.65 2.66 5.25 8.01 8.27 3.13 0.39
Mitotic Spindle -5.59 -27.66* -18.29 -15.20 8.02 0.72 -13.66 -15.44 -15.80 -1.03 -3.99 -30.08 -24.17 -22.04 5.89
None 4.65 4.87 4.96 0.88 -0.33 2.63 -2.02 2.92 0.54 4.00 3.55 -0.89 4.40 0.96 3.82
Nuclear Periphery -0.31 -6.19 -2.62 -2.57 0.91 2.30 -3.84 -3.37 -8.17 3.07 2.31 -6.17 -3.86 -4.68 3.09
Nuclear Periphery Foci 0.11 -2.17 1.25 1.10 3.29 4.91 -4.89 1.77 -3.90 6.33 3.88 -5.31 1.69 -2.24 6.73
Nucleolus 0.85 4.60 4.82 2.05 0.48 3.15 -2.71 1.88 -1.08 3.97 3.31 -1.37 3.27 0.27 3.68
Nucleus 1.72 3.42 2.38 0.57 -1.75 2.33 -5.82 2.46 -0.22 6.68 2.95 -4.11 3.39 0.04 6.06
Peroxisomes -18.14 -4.90 -11.59 5.85 -6.34 -10.06 11.77 -0.73 8.57 -11.77 -22.60 3.68 -9.69 10.60 -12.18
Vacuole -0.50 -1.25 -5.24 -5.08 -4.97 3.09 -4.61 -2.29 -3.45 1.93 2.05 -4.65 -5.69 -6.28 -3.16
Vacuole Periphery -0.41 3.05 -1.65 -1.55 -2.11 -1.06 -0.79 1.13 1.24 1.81 -0.93 2.16 -1.46 -0.53 -1.92
Standard name
Human Ortholog
Description Cytoplasmic protein of unknown function; computational analysis of large-scale protein-protein interaction data suggests a possible role in actin patch assembly
Localization
Cell Percentages peroxisome (24%), spindle pole (10%), mixed (27%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Hua2

Hua2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Hua2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available