Standard name
Human Ortholog
Description Transcriptional coactivator; bridges the DNA-binding region of Gcn4p and TATA-binding protein Spt15p; suppressor of frameshift mutations; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0.05 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.0 1.0 0.98 1.0 0.99 0.95 – 0.75 0.85 0.85 0.84 0.8 0.99 0.99 0.99 0.99 1.0 0.99 0.99 0.98 0.99 0.95 0.96 0.95
Endoplasmic Reticulum 0 0 0 0 0 0 – 0.21 0.13 0.13 0.11 0.16 0 0 0 0 0 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 2 3 6 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 9 2 1 2 – 6 3 3 1 3 3 1 1 0 0 0 0 0 0 0 4 10
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 2 3 2 7 9 – 12 14 21 16 28 3 6 6 1 2 8 0 0 0 0 0 0
Cytoplasm 256 116 192 281 503 489 – 442 436 462 307 385 541 685 732 79 217 208 247 108 187 202 413 571
Endoplasmic Reticulum 0 0 1 0 4 20 – 123 66 70 41 75 3 3 9 2 5 13 0 0 0 0 2 1
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 1 1 0 0 0 0 1 0 0 0 0 0 1 0
Mitochondria 0 0 1 1 0 0 – 0 1 0 2 0 0 1 1 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 1 0 – 2 0 0 0 1 1 1 0 0 0 0 1 0 0 2 2 3
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 6 0 0 1 2 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 1 – 5 0 0 2 2 1 0 1 1 0 1 0 0 0 3 3 7
Unique Cell Count 256 116 195 281 510 513 588 511 542 364 481 546 689 740 80 217 211 250 110 190 212 432 601
Labelled Cell Count 256 118 206 286 516 521 596 520 558 374 503 552 697 750 83 225 230 250 110 190 212 432 601


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 105.5 135.1 68.5 84.8 100.2 93.1 – 85.7 82.6 84.2 76.3 73.2 122.2 138.7 138.4 242.5 225.8 232.0 95.3 102.2 115.8
Std Deviation (1e-4) 24.4 24.5 21.2 20.0 22.0 23.7 – 24.3 25.6 25.6 26.1 25.6 30.3 33.7 33.3 140.4 105.2 124.4 28.0 27.2 33.8
Intensity Change (Log2) – – – 0.31 0.55 0.44 – 0.32 0.27 0.3 0.16 0.1 0.84 1.02 1.01 1.82 1.72 1.76 0.48 0.58 0.76


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 1.6 1.8 2.4 0 0 0 0 0 1.4
Cytoplasm 2.1 0.2 -1.9 – -7.2 -5.0 -5.0 -5.1 -6.1 0.7 1.3 0.5 0.2 1.8 0.1
Endoplasmic Reticulum 0 0 2.4 – 6.8 5.0 5.0 4.6 5.6 0 0 0 0 0 3.1
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 115.5511 142.2411 134.0408 112.3022 118.6807 133.3713 102.9408 133.2999 127.8141 116.5295 108.4039 115.3531 110.5066 139.7294 134.9398 134.0916 136.1174 138.4372
Actin 0.0009 0 0.0014 0.0003 0.0005 0 0.0047 0.0001 0.0001 0 0.0003 0.0001 0.0027 0.0001 0.0106 0 0.0008 0.0001
Bud 0.0005 0 0.0001 0.0007 0.0001 0 0.0019 0 0 0 0.0001 0.0002 0.0007 0.0001 0.0002 0.0001 0.0006 0.0002
Bud Neck 0.0003 0.0001 0.0004 0.0002 0.0003 0.0005 0.0002 0.0001 0.0001 0.0002 0.0002 0.0005 0.0004 0.0001 0.0001 0.0002 0.0004 0.0003
Bud Periphery 0.0002 0 0.0001 0.0004 0 0 0.0009 0 0 0 0.0001 0.0001 0.0004 0 0.0002 0 0.0006 0
Bud Site 0.0002 0 0.0001 0.0003 0.0003 0 0.0006 0 0.0001 0 0.0002 0.0001 0.0006 0 0.0004 0 0.0002 0
Cell Periphery 0.0013 0.0005 0.0002 0.0084 0.0004 0 0.0006 0.0001 0.0001 0 0.0005 0.0002 0.0005 0.0002 0.0001 0 0.0006 0
Cytoplasm 0.9477 0.9959 0.9768 0.9123 0.9555 0.995 0.8965 0.9979 0.9925 0.995 0.8805 0.9343 0.8774 0.997 0.9739 0.9952 0.9538 0.9954
Cytoplasmic Foci 0.0025 0.0001 0.003 0.0036 0.0103 0.0001 0.0139 0.0001 0.0014 0.0001 0.0417 0.006 0.0185 0.0001 0.0016 0.0002 0.0047 0.0004
Eisosomes 0.0002 0 0.0001 0.0005 0.0009 0 0.0004 0 0 0 0.0005 0.0001 0.0002 0 0.0001 0 0.0003 0
Endoplasmic Reticulum 0.0013 0.0008 0.0007 0.0005 0.0007 0.0005 0.0017 0.0001 0.0002 0.0001 0.0002 0.0002 0.0013 0.0001 0.0001 0 0.0007 0.0001
Endosome 0.0022 0 0.0015 0.0004 0.0004 0 0.0068 0 0 0.0001 0.0006 0.0001 0.0047 0 0.0005 0.0001 0.0005 0.0001
Golgi 0.0003 0 0.0002 0.0001 0.0004 0 0.002 0 0 0 0.0003 0 0.0015 0 0.0007 0 0.0001 0
Lipid Particles 0.0013 0 0.0014 0.0015 0.0084 0 0.0056 0 0.0006 0 0.0102 0.0006 0.0062 0 0.0039 0 0.0012 0
Mitochondria 0.0014 0 0.0019 0.0101 0.0034 0 0.005 0 0.0004 0 0.0103 0.0044 0.0052 0 0.0006 0 0.0052 0
None 0.0045 0.0001 0.0015 0.0036 0.0061 0.0001 0.0104 0.0001 0.0008 0.0001 0.0117 0.0059 0.0144 0.0002 0.0001 0.0001 0.0214 0.0001
Nuclear Periphery 0.0056 0.0005 0.0014 0.0021 0.0017 0.0009 0.0112 0.0004 0.0005 0.0011 0.0027 0.0009 0.0069 0.0005 0.0006 0.0007 0.001 0.0006
Nucleolus 0.0073 0 0.0023 0.017 0.0032 0 0.0086 0 0.001 0 0.0214 0.0323 0.005 0 0 0 0.0013 0
Nucleus 0.0102 0.0011 0.0034 0.0127 0.0027 0.0022 0.015 0.0009 0.0013 0.0019 0.0072 0.0109 0.0272 0.0014 0.0013 0.0023 0.0025 0.0022
Peroxisomes 0.0001 0 0.0002 0.0003 0.0006 0 0.0011 0 0.0001 0 0.001 0.0002 0.0114 0 0.0042 0 0.0006 0
Punctate Nuclear 0.0019 0 0.0005 0.0002 0.0008 0 0.0069 0 0.0002 0 0.0025 0.0016 0.0114 0 0.0006 0 0.0004 0
Vacuole 0.0082 0.0007 0.0015 0.0197 0.0019 0.0004 0.0036 0.0003 0.0005 0.0011 0.0028 0.0007 0.0026 0.0002 0.0002 0.0007 0.0022 0.0003
Vacuole Periphery 0.0019 0 0.0013 0.0054 0.0015 0.0001 0.0024 0 0.0002 0.0002 0.0051 0.0004 0.0009 0 0 0.0003 0.001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 251.6536 616.4851 396.4641 431.0901 373.3396 394.2003 547.872 574.9622 564.0357 386.5806
Translational Efficiency 2.6502 2.3261 2.1359 2.458 2.8252 2.6077 2.4133 2.7941 2.3946 2.504

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
22 175 226 89 1636 1598 2840 47 1658 1773 3066 136

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 18559.95 6061.46 12349.57 9762.59 4626.37 4978.96 9337.14 19648.37 4811.25 5085.81 9559.19 13179.00
Standard Deviation 5220.36 2279.69 2945.48 1895.92 1200.86 1206.61 2071.90 5269.77 2080.01 1389.03 2288.11 5835.36
Intensity Change Log 2 — -1.614456 -0.587732 -0.926857 — 0.105964 1.013100 2.086457 — -1.070479 -0.096463 0.343080

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000371 0.000099 0.000136 0.000139 0.000035 0.000078 0.000028 0.000160 0.000040 0.000080 0.000036 0.000146
Bud Neck 0.005754 0.001176 0.004256 0.002810 0.000166 0.000669 0.000506 0.002873 0.000240 0.000719 0.000783 0.002832
Bud Site 0.003981 0.004295 0.001245 0.002421 0.001009 0.001235 0.001627 0.006998 0.001048 0.001537 0.001599 0.004003
Cell Periphery 0.144541* 0.005576 0.024201 0.057748 0.002924 0.003128 0.001460 0.037728 0.004803 0.003370 0.003137 0.050829
Cytoplasm 0.278710* 0.831602* 0.671026* 0.568619* 0.923363* 0.880008* 0.903746* 0.551972* 0.914809* 0.875230* 0.886592* 0.562866*
Cytoplasmic Foci 0.031107 0.028076 0.023786 0.009098 0.002791 0.007597 0.003217 0.009313 0.003167 0.009619 0.004733 0.009172
Eisosomes 0.000018 0.000002 0.000009 0.000009 0.000000 0.000001 0.000001 0.000010 0.000001 0.000001 0.000001 0.000009
Endoplasmic Reticulum 0.000534 0.000143 0.000065 0.000067 0.000098 0.000093 0.000074 0.000150 0.000104 0.000098 0.000073 0.000096
Endosome 0.000610 0.001742 0.000178 0.000141 0.000077 0.000257 0.000129 0.000166 0.000084 0.000403 0.000133 0.000150
Golgi 0.056333 0.015150 0.006244 0.004500 0.005046 0.007740 0.001292 0.002514 0.005726 0.008471 0.001657 0.003813
Lipid Particles 0.076193 0.012984 0.014347 0.013918 0.002328 0.002823 0.001776 0.024527 0.003308 0.003826 0.002702 0.017584
Mitochondria 0.000335 0.000598 0.000131 0.000113 0.000088 0.000151 0.000107 0.000064 0.000091 0.000195 0.000109 0.000096
Mitotic Spindle 0.004679 0.005490 0.000439 0.000093 0.000162 0.000820 0.000584 0.000085 0.000221 0.001281 0.000573 0.000090
None 0.002918 0.008436 0.004108 0.001857 0.005986 0.007026 0.003273 0.001727 0.005945 0.007166 0.003334 0.001812
Nuclear Periphery 0.005416 0.000246 0.003109 0.001264 0.000134 0.000228 0.000146 0.000649 0.000204 0.000230 0.000365 0.001052
Nuclear Periphery Foci 0.032412 0.004353 0.007477 0.003436 0.002938 0.004352 0.001283 0.003716 0.003329 0.004352 0.001740 0.003533
Nucleolus 0.000290 0.000084 0.000098 0.000110 0.000048 0.000081 0.000105 0.000159 0.000051 0.000081 0.000105 0.000127
Nucleus 0.050898 0.038699 0.130216* 0.079511 0.029976 0.053568 0.044103 0.055000 0.030254 0.052100 0.050450 0.071041
Peroxisomes 0.000020 0.000066 0.000008 0.000008 0.000006 0.000005 0.000019 0.000033 0.000006 0.000011 0.000018 0.000017
Vacuole 0.239572* 0.034723 0.103254 0.245135* 0.015114 0.026515 0.034809 0.299282* 0.018092 0.027325 0.039854 0.263848*
Vacuole Periphery 0.065307 0.006460 0.005668 0.009003 0.007711 0.003624 0.001715 0.002875 0.008475 0.003904 0.002007 0.006885

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 2.70 2.23 2.15 -1.45 -0.09 -2.34 0.15 -3.48 -1.97 -3.85 -2.35 -0.17 -4.93 -3.07 -5.63
Bud Neck 1.85 0.53 1.37 -2.63 2.77 -5.80 -8.21 -3.30 -2.73 -2.79 -5.66 -8.58 -6.30 -4.89 -4.51
Bud Site -1.06 2.94 -0.52 0.42 -1.41 -1.14 -1.51 -2.56 -2.30 -2.17 -1.96 -1.45 -2.29 -1.76 -1.96
Cell Periphery 4.28 3.60 2.71 -4.75 -2.46 -0.51 3.40 -4.13 -4.10 -4.36 1.78 1.88 -5.84 -6.06 -6.07
Cytoplasm -10.49 -6.60 -5.00 6.66 1.40 9.86 9.33 9.74 8.48 8.75 9.02 10.47 14.56 12.51 12.48
Cytoplasmic Foci -0.30 -0.15 2.55 3.93 5.27 -7.44 -2.66 -3.65 -1.11 -3.12 -8.66 -5.24 -4.95 0.10 -3.11
Eisosomes 2.71 1.34 1.71 -5.21 1.31 -3.71 -1.84 -4.08 -3.81 -3.99 -2.64 -3.73 -7.48 -7.03 -6.86
Endoplasmic Reticulum 1.87 2.31 2.26 0.83 -0.46 0.23 0.73 0.21 0.10 -0.20 0.28 0.88 0.65 0.65 -0.22
Endosome -1.21 2.65 2.73 1.73 0.46 -4.63 -4.58 -2.27 1.37 -0.73 -3.74 -4.58 -3.06 2.24 -1.04
Golgi 2.66 3.40 3.57 2.49 2.02 -3.76 6.90 4.16 8.35 -1.80 -3.42 6.90 2.84 6.30 -3.34
Lipid Particles 3.43 3.30 3.51 -0.16 1.29 -1.41 0.35 -5.15 -5.00 -5.20 -1.11 -0.16 -7.19 -6.74 -7.31
Mitochondria -0.38 2.25 2.68 0.97 1.57 -2.83 -1.54 0.66 2.51 1.65 -2.19 -1.59 -0.60 1.87 1.04
Mitotic Spindle -0.30 2.39 2.75 1.35 4.43 -2.02 -2.79 2.00 2.24 3.11 -2.27 -2.71 2.59 2.51 3.19
None -2.33 -1.17 1.46 2.96 3.88 -0.69 3.40 5.53 5.20 3.89 -0.82 3.37 5.62 5.68 4.64
Nuclear Periphery 4.88 1.46 3.93 -3.42 3.61 -5.15 -1.98 -4.28 -3.46 -3.30 -1.56 -3.93 -4.63 -4.40 -2.83
Nuclear Periphery Foci 1.88 1.64 1.96 1.36 3.88 -3.26 4.47 -1.29 -0.18 -2.28 -2.36 3.35 -0.85 0.92 -3.12
Nucleolus 1.65 1.58 1.58 -0.55 -0.06 -2.41 -5.45 -2.54 -1.25 -0.17 -2.37 -5.22 -3.88 -1.66 0.22
Nucleus 0.98 -3.22 -0.46 -3.30 5.32 -10.97 -10.86 -3.15 -0.22 -0.43 -10.84 -13.46 -5.91 -2.39 -1.57
Peroxisomes -2.00 1.89 0.65 2.16 -0.91 0.35 -3.56 -2.57 -2.89 -0.91 -1.55 -3.44 -2.38 -1.09 -0.15
Vacuole 4.95 3.05 -0.03 -6.98 -4.25 -5.74 -11.37 -8.14 -7.74 -7.42 -5.15 -11.63 -11.65 -10.97 -10.24
Vacuole Periphery 2.54 2.58 2.37 -0.70 -0.97 2.58 4.06 3.19 1.24 -0.81 2.83 4.28 0.69 -1.21 -2.10
Standard name
Human Ortholog
Description Transcriptional coactivator; bridges the DNA-binding region of Gcn4p and TATA-binding protein Spt15p; suppressor of frameshift mutations; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages cytoplasm (99%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-2

Mbf1

Mbf1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mbf1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available