Standard name
Human Ortholog
Description Small subunit of carbamoyl phosphate synthetase; carbamoyl phosphate synthetase catalyzes a step in the synthesis of citrulline, an arginine precursor; translationally regulated by an attenuator peptide encoded by YOR302W within the CPA1 mRNA 5'-leader
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0.09 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0.05 0.08 0.06 0.09 0.17 0.1 0 0 0 0.07 0 0 0 0 0 0 0 0
Cytoplasm 1.0 0.99 0.99 0.99 0.98 0.93 0.89 0.78 0.88 0.73 0.71 0.73 0.99 0.99 0.99 0.97 0.99 0.98 0.98 0.97 0.94 0.99 0.97 0.96
Endoplasmic Reticulum 0 0 0 0 0 0 0.09 0.15 0.07 0.13 0.07 0.12 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0.09 0.13 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 1 5 3 10 16 8 0 0 0 0 0 0 0 0 0 0 0 0
Bud 1 1 0 0 0 0 1 1 1 7 1 2 0 0 2 0 0 0 3 1 0 0 2 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 3 4 7 3 5 7 16 24 9 22 30 19 7 7 10 20 10 8 0 0 0 0 0 0
Cytoplasm 405 148 182 108 191 212 264 234 143 175 125 139 204 271 346 272 255 176 386 140 167 159 223 272
Endoplasmic Reticulum 2 1 1 1 3 9 26 45 11 31 12 23 2 1 0 9 9 6 0 1 6 0 0 1
Endosome 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 2 2 3 0 1 3 1 1 0 0 0 1 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 4 4 12 6 21 23 18 0 0 0 1 0 0 0 0 1 0 0 1
Nucleus 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0 1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 1 1 0 0 4 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 1 0 0 0 1 6 1 6 0 5 0 2 0 3 2 3 0 0 0 0 2 0
Unique Cell Count 407 149 184 109 194 227 296 300 162 241 175 190 206 273 348 281 258 179 396 145 177 162 231 285
Labelled Cell Count 412 154 191 112 199 235 316 331 174 274 214 215 214 281 358 306 277 193 396 145 177 162 231 285


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 70.2 72.8 90.3 72.4 56.1 38.5 29.9 26.5 22.9 20.9 20.0 20.8 90.3 95.6 101.9 36.7 34.4 36.5 94.0 86.4 84.3
Std Deviation (1e-4) 14.3 14.5 15.9 16.0 13.4 8.9 6.8 7.1 5.4 5.2 6.3 6.0 21.6 26.2 27.9 11.4 9.7 13.3 22.6 20.7 20.4
Intensity Change (Log2) – – – -0.32 -0.69 -1.23 -1.59 -1.77 -1.98 -2.11 -2.17 -2.12 0.0 0.08 0.17 -1.3 -1.39 -1.31 0.06 -0.06 -0.1


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 4.2 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 -0.7 -0.4 0.8 1.8 0.8 2.2 4.2 2.4 -0.2 -0.8 -0.6 1.5 0 0.3
Cytoplasm 0.1 -0.4 -2.8 -4.0 -6.4 -4.1 -7.3 -7.4 -7.1 0.1 0.4 0.7 -1.5 -0.1 -0.5
Endoplasmic Reticulum 0 0 0 3.8 5.3 3.2 4.8 3.2 4.6 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 4.1 5.1 4.3 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 37.7792 51.2322 46.7995 42.351 40.1567 47.4795 49.8513 78.2838 66.2502 61.5007 47.3759 68.237 63.1913 74.0452 73.3668 74.7205 64.3477 69.3175
Actin 0.0135 0.0017 0.0015 0.0002 0.0023 0.006 0.0465 0.0044 0.0074 0.0017 0.0673 0.0128 0.04 0.0001 0.0002 0.0001 0.0001 0.0089
Bud 0.0003 0.0002 0.0031 0.0043 0.0002 0.0029 0.0023 0.0001 0.0024 0.0003 0.0072 0.0002 0.0008 0.0002 0.0003 0 0.0003 0.0004
Bud Neck 0.0005 0.0002 0.0004 0.0006 0.0012 0.0007 0.0026 0.0002 0.0005 0.0004 0.0023 0.0007 0.0007 0.0001 0.0002 0.0001 0.0002 0.0008
Bud Periphery 0.0003 0.0001 0.0022 0.0028 0.0002 0.0062 0.0024 0.0001 0.0032 0.0002 0.0117 0.0001 0.0011 0.0001 0.0002 0 0.0002 0.0008
Bud Site 0.0003 0.0005 0.0003 0.0004 0.0002 0.0002 0.003 0.0001 0.0021 0.0008 0.0102 0.0001 0.0016 0.0001 0.0002 0 0.0001 0.0001
Cell Periphery 0.0007 0.0003 0.0004 0.0007 0.0007 0.0002 0.0013 0.0005 0.0003 0.0001 0.0042 0.0001 0.0006 0.002 0.0003 0 0.0005 0.0004
Cytoplasm 0.9304 0.979 0.9606 0.9321 0.9303 0.9175 0.7404 0.981 0.914 0.8561 0.8031 0.9559 0.8866 0.9808 0.9763 0.9966 0.9611 0.9704
Cytoplasmic Foci 0.0034 0.0115 0.0044 0.001 0.0017 0.0025 0.0272 0.0018 0.0069 0.0063 0.0081 0.0033 0.0043 0.0009 0.0015 0.0002 0.0012 0.0011
Eisosomes 0.0002 0 0 0 0 0 0.0006 0 0.0001 0 0.0012 0 0.0008 0 0 0 0 0.0001
Endoplasmic Reticulum 0.0055 0.0014 0.0047 0.0072 0.023 0.0017 0.0172 0.0006 0.0045 0.0045 0.0041 0.0008 0.0032 0.0009 0.0008 0.0006 0.0015 0.0025
Endosome 0.0042 0.0005 0.0023 0.0025 0.0114 0.0027 0.0336 0.002 0.0122 0.0457 0.0155 0.0029 0.0047 0.0009 0.0009 0.0001 0.0016 0.0036
Golgi 0.0009 0.0003 0.0007 0.0001 0.0016 0.0016 0.0064 0.0051 0.0033 0.01 0.0143 0.004 0.0034 0.0001 0.0003 0 0 0.0031
Lipid Particles 0.0024 0.0008 0.0046 0.0003 0.0049 0.0047 0.0124 0.0003 0.0022 0.0012 0.0025 0.0051 0.0031 0.0003 0.0009 0 0.0001 0.0004
Mitochondria 0.0027 0.0001 0.0008 0.0001 0.0003 0.0167 0.0081 0.0007 0.0075 0.0043 0.0252 0.0009 0.0122 0.0007 0.0018 0 0.0008 0.0022
None 0.0058 0.0005 0.0003 0.0002 0.0006 0.0006 0.0168 0.0004 0.0093 0.0004 0.006 0.0005 0.0073 0.0019 0.0005 0.0001 0.0032 0.0005
Nuclear Periphery 0.0052 0.0005 0.0067 0.0056 0.0113 0.0031 0.0229 0.0005 0.0111 0.0449 0.0027 0.0037 0.0074 0.0012 0.0012 0.0004 0.0027 0.0012
Nucleolus 0.0028 0 0.0006 0.0001 0.0001 0.0057 0.0012 0 0.0009 0.0002 0.0006 0.0003 0.0033 0.0005 0.0001 0 0.0003 0
Nucleus 0.0057 0.0008 0.0021 0.0047 0.0038 0.0091 0.0091 0.0007 0.0041 0.0147 0.0031 0.0047 0.0087 0.0023 0.0021 0.0007 0.0045 0.0011
Peroxisomes 0.0008 0.0001 0.0003 0 0.0001 0.0123 0.0094 0.0002 0.0008 0.0003 0.0016 0.0017 0.0021 0 0.0049 0 0 0.0001
Punctate Nuclear 0.0023 0.0002 0.0005 0.0001 0.0005 0.0009 0.006 0.0001 0.0015 0.0005 0.0007 0.0009 0.0044 0 0.0001 0 0.0002 0.0001
Vacuole 0.0099 0.0012 0.0028 0.0315 0.0048 0.0031 0.025 0.001 0.0039 0.0038 0.007 0.0008 0.0022 0.0053 0.0052 0.0008 0.0183 0.0017
Vacuole Periphery 0.0021 0.0001 0.0008 0.0053 0.0008 0.0017 0.0058 0.0002 0.0018 0.0035 0.0015 0.0004 0.0016 0.0015 0.0019 0.0001 0.003 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 149.9773 124.4941 110.1454 97.2063 85.6515 244.4704 283.6241 259.7745 233.2494 203.7525
Translational Efficiency 0.7298 0.6718 0.7009 0.6721 0.6367 0.9593 0.9125 1.0191 0.9408 0.8232

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Small subunit of carbamoyl phosphate synthetase; carbamoyl phosphate synthetase catalyzes a step in the synthesis of citrulline, an arginine precursor; translationally regulated by an attenuator peptide encoded by YOR302W within the CPA1 mRNA 5'-leader
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-2

Cpa1

Cpa1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cpa1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available