Standard name
Human Ortholog
Description ATP-dependent DNA translocase involved in chromatin remodeling; ATPase component that, with Itc1p, forms a complex required for repression of a-specific genes, INO1, and early meiotic genes during mitotic growth; the Isw2 complex exhibits basal levels of chromatin binding throughout the genome as well as target-specific chromatin interactions; targeted by Ume6p- and Sua7p-dependent DNA looping to many loci genome-wide
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0.05 – 0 0.06 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.08 0.05 0.07 0.07 0 – 0.06 0 0.12 0.16 0.19 0.05 0.05 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.19 0.31 0.46 0.14 0.43 0.36 0.33 0.49 – 0.46 0.5 0 0 0 0.05 0 0.11 0 0 0 0 0 0
Nucleus 0.95 0.92 0.93 0.91 0.87 0.81 0.81 0.83 0.8 – 0.7 0.81 0.86 0.82 0.76 0.86 0.87 0.91 0.89 0.89 0.92 0.86 0.66 0.57
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.09 0 0 0.05 0.05 0.1 0.08 0.16 0.18 – 0.24 0.21 0 0 0.08 0 0 0.06 0.06 0 0 0 0.15 0.18
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.05 0 0 0 0 0.06 0.1 0.14
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Bud 0 1 0 0 0 1 0 4 5 – 3 1 0 0 0 1 0 1 0 2 0 0 1 1
Bud Neck 0 0 0 0 0 0 1 0 1 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 1 1 0 3 6 5 9 15 – 6 14 0 0 1 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1 6 1 4 9 11 20 21 11 – 12 7 26 37 38 20 12 12 0 0 0 0 1 0
Endoplasmic Reticulum 1 0 0 0 0 0 0 0 0 – 0 0 1 0 0 9 4 3 0 0 0 0 0 1
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 4 2 3 0 3 0 2 3 4
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 2 1 1 0 1 0 1 1 1
Mitochondria 3 53 65 64 17 101 107 104 154 – 89 108 3 6 9 17 3 32 0 3 0 1 4 6
Nucleus 121 260 194 128 104 191 243 259 251 – 134 175 190 186 155 312 205 274 122 259 194 178 149 141
Nuclear Periphery 0 2 0 1 3 2 2 1 5 – 4 1 0 3 1 1 5 4 0 0 1 0 0 1
Nucleolus 12 6 6 7 6 23 24 49 58 – 47 46 7 10 17 10 8 17 8 5 5 7 33 45
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 1 0 1 0 0 0 0 0 0 0 1 0
SpindlePole 1 1 2 0 0 1 1 3 0 – 0 0 1 1 1 7 2 5 1 2 1 0 2 4
Vac/Vac Membrane 1 2 0 0 0 1 2 2 0 – 0 2 1 0 2 12 12 2 2 11 4 13 22 34
Unique Cell Count 128 282 209 140 119 235 300 311 314 192 217 222 228 203 364 235 300 137 291 212 207 227 247
Labelled Cell Count 140 332 269 204 142 337 405 452 500 295 354 230 243 225 395 254 354 137 291 212 207 227 247


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.8 5.6 4.7 4.5 5.3 4.4 4.6 4.4 4.0 – 4.0 3.7 6.6 6.1 5.7 7.6 8.8 7.8 7.1 7.4 7.5
Std Deviation (1e-4) 1.7 1.1 1.0 0.8 2.0 1.0 1.5 1.4 1.0 – 1.2 0.9 1.6 1.5 1.5 1.8 2.1 1.7 1.8 1.7 1.3
Intensity Change (Log2) – – – -0.07 0.17 -0.09 -0.04 -0.08 -0.24 – -0.21 -0.32 0.5 0.37 0.28 0.7 0.91 0.73 0.6 0.65 0.68


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 0 0 2.7 3.5 3.5 0 – 3.3 0 4.8 5.8 6.3 3.1 2.9 2.5
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0 -3.4 0 0 0 0 – 0 0 -8.5 -8.1 -7.0 -8.7 -8.7 -5.8
Nucleus -0.5 -1.6 -3.6 -3.8 -3.2 -4.1 – -6.0 -3.7 -2.4 -3.5 -4.6 -2.5 -1.9 -0.6
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0.2 0.8 2.4 -0.1 0.3 1.5
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 – 0 0 0 0 0 0 3.3 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.6246 3.2338 2.9227 2.7382 3.2505 3.242 1.7693 1.0721 1.1712 0.9381 0.8092 1.2451 6.1137 5.5887 6.0301 6.064 5.5472 5.7178
Actin 0.0073 0 0.0042 0.0079 0.0429 0.0003 0.0025 0 0.0013 0.0003 0.0044 0.0022 0 0 0 0 0 0
Bud 0.0003 0 0.0003 0.0001 0.0003 0 0.0002 0 0 0.0001 0.0001 0 0 0 0 0 0 0
Bud Neck 0.0035 0.0001 0.0008 0.0003 0.0006 0.0006 0.0008 0 0.0007 0.0003 0.001 0.0008 0 0.0001 0 0 0.0003 0.0002
Bud Periphery 0.0003 0 0.0006 0.0002 0.0006 0.0002 0.0003 0 0.0002 0.0001 0.0003 0.0001 0 0 0 0 0 0
Bud Site 0.0051 0.0001 0.0023 0.0001 0.0011 0.0001 0.0003 0 0.0001 0.0002 0.0002 0.0001 0 0 0 0 0 0
Cell Periphery 0.0002 0 0.0001 0 0.0001 0 0.0001 0 0.0004 0.0001 0.0001 0 0 0 0 0 0 0
Cytoplasm 0.0022 0.0007 0.0014 0.0002 0.0003 0 0.0007 0 0.0006 0.0002 0.0008 0.0001 0 0 0 0.0001 0.0001 0
Cytoplasmic Foci 0.0055 0 0.0032 0.0062 0.0017 0 0.0024 0 0 0.0016 0.0006 0.0001 0 0 0 0 0 0
Eisosomes 0.0001 0 0.0002 0 0.0003 0 0 0 0.0002 0 0.0001 0.0001 0 0 0 0 0 0
Endoplasmic Reticulum 0.0008 0 0.0007 0.0001 0.0012 0.0003 0.0006 0 0.002 0.0003 0.0014 0.0006 0 0 0 0 0 0
Endosome 0.0051 0 0.0061 0.0012 0.0055 0.0005 0.0083 0 0.0004 0.0181 0.0092 0.0014 0 0 0 0 0.0001 0
Golgi 0.0028 0 0.002 0.0016 0.0042 0.0001 0.0021 0 0.0001 0.0032 0.0013 0.0005 0 0 0 0 0 0
Lipid Particles 0.0042 0 0.0055 0.0077 0.0051 0.0003 0.0109 0 0 0.0063 0.0042 0.0005 0 0 0 0 0 0
Mitochondria 0.0015 0.0001 0.0074 0.0005 0.0073 0.0023 0.0025 0 0.0007 0.0021 0.0028 0.0012 0 0 0 0 0.0002 0
None 0.0014 0 0.0025 0.0003 0.0007 0.0001 0.0006 0 0.0001 0.0002 0.0007 0.0001 0 0 0 0 0 0
Nuclear Periphery 0.01 0.0004 0.0085 0.0005 0.0141 0.0028 0.0086 0.0021 0.0046 0.0014 0.0573 0.0062 0.0001 0.0002 0.0002 0.0005 0.003 0.0001
Nucleolus 0.0302 0.0167 0.0204 0.0122 0.0532 0.0446 0.0107 0.0119 0.011 0.0099 0.0576 0.0232 0.0096 0.0198 0.0105 0.0093 0.052 0.0271
Nucleus 0.9009 0.9813 0.9158 0.9409 0.8444 0.9467 0.9428 0.9816 0.9763 0.9507 0.8152 0.9606 0.9897 0.9794 0.9794 0.9894 0.9397 0.9723
Peroxisomes 0.0066 0 0.0041 0.0155 0.002 0 0.0029 0 0 0.0004 0.0004 0 0 0 0 0 0 0
Punctate Nuclear 0.0104 0.0005 0.0117 0.0041 0.013 0.0005 0.0012 0.0042 0.0004 0.0008 0.0361 0.0016 0.0005 0.0005 0.0098 0.0006 0.0043 0.0003
Vacuole 0.001 0.0001 0.0011 0.0004 0.0005 0.0003 0.0008 0 0.0004 0.0023 0.0031 0.0002 0 0 0 0 0.0001 0
Vacuole Periphery 0.0006 0 0.001 0.0002 0.0009 0.0004 0.0007 0 0.0003 0.0014 0.0029 0.0004 0 0 0 0 0.0001 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 15.5848 15.8383 10.8822 24.7165 17.654 12.133 16.6814 21.717 22.6722 22.9208
Translational Efficiency 0.7397 0.749 1.2407 0.5994 0.7472 0.8512 0.646 0.4885 0.5945 0.6265

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
690 1136 266 1117 959 1548 153 145 1649 2684 419 1262

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 659.75 798.85 1167.34 1027.30 821.95 825.51 1136.65 1049.22 754.08 814.23 1156.13 1029.82
Standard Deviation 89.16 132.51 151.50 180.92 107.20 126.43 148.87 149.44 128.11 129.71 151.27 177.72
Intensity Change Log 2 — 0.276005 0.823233 0.638866 — 0.006235 0.467666 0.352195 — 0.132618 0.636881 0.486914

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000209 0.000708 0.001801 0.001069 0.000307 0.000454 0.001512 0.001326 0.000266 0.000562 0.001696 0.001099
Bud Neck 0.002120 0.009572 0.015097 0.027072 0.004021 0.009961 0.009309 0.018508 0.003225 0.009797 0.012983 0.026088
Bud Site 0.002552 0.001816 0.001447 0.003609 0.000479 0.001358 0.001139 0.014425 0.001346 0.001552 0.001334 0.004851
Cell Periphery 0.000174 0.000125 0.000042 0.000122 0.000039 0.000107 0.000039 0.000070 0.000096 0.000115 0.000041 0.000116
Cytoplasm 0.005291 0.003677 0.000295 0.004667 0.000232 0.001690 0.000179 0.001553 0.002349 0.002531 0.000253 0.004309
Cytoplasmic Foci 0.000835 0.000665 0.000105 0.001036 0.000045 0.000804 0.000115 0.000068 0.000376 0.000745 0.000108 0.000925
Eisosomes 0.000018 0.000030 0.000042 0.000038 0.000023 0.000028 0.000065 0.000039 0.000021 0.000029 0.000051 0.000038
Endoplasmic Reticulum 0.001769 0.004827 0.005155 0.003146 0.002843 0.004158 0.005258 0.004523 0.002393 0.004441 0.005193 0.003304
Endosome 0.000244 0.000679 0.000094 0.001771 0.000075 0.000480 0.000186 0.000179 0.000146 0.000564 0.000128 0.001588
Golgi 0.000115 0.000590 0.000098 0.001386 0.000111 0.000921 0.000113 0.000097 0.000113 0.000781 0.000103 0.001238
Lipid Particles 0.000298 0.000217 0.000300 0.000171 0.000072 0.000401 0.000667 0.000150 0.000166 0.000323 0.000434 0.000169
Mitochondria 0.001147 0.002084 0.001153 0.005787 0.000537 0.003272 0.001434 0.001793 0.000792 0.002769 0.001255 0.005328
Mitotic Spindle 0.000757 0.001932 0.000144 0.022031 0.001468 0.005403 0.000946 0.001756 0.001171 0.003934 0.000437 0.019702
None 0.017925 0.006692 0.000824 0.007064 0.001486 0.002915 0.000914 0.002846 0.008365 0.004513 0.000857 0.006579
Nuclear Periphery 0.000234 0.000316 0.000110 0.000866 0.000120 0.000464 0.000213 0.000352 0.000167 0.000402 0.000148 0.000807
Nuclear Periphery Foci 0.000809 0.000786 0.000054 0.000525 0.000084 0.000864 0.001126 0.000057 0.000387 0.000831 0.000445 0.000472
Nucleolus 0.029224 0.024999 0.015555 0.017009 0.027581 0.028763 0.021276 0.008465 0.028268 0.027170 0.017644 0.016027
Nucleus 0.934907* 0.937858* 0.955551* 0.898334* 0.959771* 0.935316* 0.952690* 0.941454* 0.949367* 0.936392* 0.954506* 0.903288*
Peroxisomes 0.000838 0.001278 0.001085 0.000449 0.000433 0.000999 0.001267 0.000558 0.000602 0.001117 0.001151 0.000461
Vacuole 0.000415 0.000777 0.000937 0.002898 0.000165 0.001238 0.001401 0.001636 0.000270 0.001043 0.001106 0.002753
Vacuole Periphery 0.000119 0.000371 0.000113 0.000949 0.000108 0.000404 0.000150 0.000144 0.000113 0.000390 0.000127 0.000856

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -6.90 -2.90 -16.57 -7.26 0.65 -1.71 -5.56 -5.63 -5.26 -0.52 -5.53 -4.09 -15.54 -11.74 0.73
Bud Neck -8.06 -8.01 -16.51 -11.60 -6.48 -7.10 -6.00 -6.81 -4.80 -4.38 -10.57 -9.21 -17.14 -12.71 -8.62
Bud Site 0.45 0.41 -1.08 -4.01 -4.26 -3.32 -3.23 -1.70 -1.53 -1.52 -0.33 -0.61 -3.58 -4.28 -4.05
Cell Periphery 1.12 3.33 1.59 0.72 -4.91 -2.85 -0.20 -3.51 0.48 -3.55 -0.85 3.00 -0.68 0.32 -5.68
Cytoplasm 1.40 4.75 0.73 -0.58 -3.82 -3.17 -0.54 -2.16 -0.53 -2.02 -0.39 4.43 -1.69 -1.45 -4.06
Cytoplasmic Foci 0.20 1.66 0.32 0.17 -2.06 -2.55 -0.60 -0.15 2.54 0.55 -1.38 1.50 -0.68 0.52 -2.02
Eisosomes -5.15 -7.75 -13.11 -6.02 0.04 -1.95 -7.06 -5.01 -4.28 2.09 -4.58 -9.76 -12.59 -8.60 2.47
Endoplasmic Reticulum -10.13 -1.98 -8.08 2.96 0.65 -3.21 -3.18 -3.39 -1.78 -0.22 -7.86 -2.51 -6.24 1.41 0.95
Endosome -1.73 1.39 -2.58 -1.10 -3.01 -4.58 -1.40 -3.11 1.48 -0.83 -3.48 -0.13 -2.97 -1.51 -2.95
Golgi -1.44 -0.43 -1.63 -0.47 -1.60 -1.45 0.04 -0.63 1.38 -1.28 -1.92 -0.12 -1.62 -0.02 -1.62
Lipid Particles 0.50 0.29 1.03 0.63 0.27 -1.76 -1.05 -1.68 0.98 0.81 -1.39 -0.95 -0.37 1.22 0.86
Mitochondria -1.75 -0.23 -3.93 -2.98 -4.20 -3.44 -4.23 -7.02 1.17 -2.43 -3.87 -2.71 -4.84 -2.03 -4.21
Mitotic Spindle -1.38 -0.01 -6.47 -6.05 -6.51 -1.99 -0.47 -1.31 0.47 -0.60 -2.34 -0.19 -6.34 -5.47 -6.25
None 3.86 6.06 4.16 0.72 -4.11 -2.63 1.04 -2.24 -0.33 -3.17 3.04 6.07 2.02 -0.76 -4.35
Nuclear Periphery -1.22 1.21 -6.04 -5.71 -6.82 -3.92 -1.97 -5.07 -1.91 -3.37 -4.01 -0.81 -7.10 -5.27 -6.87
Nuclear Periphery Foci -0.30 1.30 0.16 0.68 -2.96 -1.99 -0.92 -0.07 2.00 0.91 -1.51 -0.10 -0.81 0.93 -0.47
Nucleolus 1.44 2.36 1.15 -0.24 -1.57 -0.46 1.52 6.82 7.73 3.09 0.64 2.78 1.83 1.43 -1.18
Nucleus -0.47 -1.88 6.56 7.86 8.28 5.54 1.75 3.21 0.69 1.67 3.39 0.15 10.22 8.18 8.94
Peroxisomes -0.67 -0.19 0.55 1.68 1.03 -1.23 -2.13 -0.51 0.97 1.87 -1.31 -1.18 0.38 2.03 1.88
Vacuole -0.47 -2.03 -3.79 -3.67 -2.98 -2.84 -4.00 -3.48 -2.18 -1.61 -2.52 -4.41 -4.55 -3.64 -3.06
Vacuole Periphery -2.52 -0.36 -2.77 -1.32 -2.72 -3.33 -1.56 -2.83 2.46 -0.65 -4.17 -1.33 -2.85 -1.17 -2.69
Standard name
Human Ortholog
Description ATP-dependent DNA translocase involved in chromatin remodeling; ATPase component that, with Itc1p, forms a complex required for repression of a-specific genes, INO1, and early meiotic genes during mitotic growth; the Isw2 complex exhibits basal levels of chromatin binding throughout the genome as well as target-specific chromatin interactions; targeted by Ume6p- and Sua7p-dependent DNA looping to many loci genome-wide
Localization
Cell Percentages nucleus (99%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Isw2

Isw2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Isw2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available