Standard name
Human Ortholog
Description Protein involved in ER-to-Golgi transport
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0 0 0 0.09 0 0 0 0 0 0 0 0 0.07 0.05 0.07 – – – 0 0 0 0 0 0
Cytoplasm 0.27 0.38 0.2 0.38 0.5 0.44 0.51 0.48 0.53 0.44 0.43 0.47 0.54 0.74 0.62 – – – 0.11 0.12 0 0.05 0.09 0.1
Endoplasmic Reticulum 0.7 0.61 0.7 0.39 0.25 0.17 0 0 0 0 0 0 0.44 0.24 0.36 – – – 0.77 0.74 0.88 0.81 0.73 0.7
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Mitochondria 0 0 0.07 0.22 0.17 0.41 0.49 0.54 0.57 0.7 0.69 0.63 0 0 0 – – – 0 0 0 0.06 0.06 0.05
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0.07 0.11 0.14 0.12 0.1 0.09 0.09 0.07 0 0.06 0 0 0 – – – 0 0.05 0 0 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 6 3 10 5 1 0 0 0 0 0 0 0 1 2 1 – – – 4 5 11 4 15 11
Bud 0 3 2 1 1 3 4 21 14 16 20 16 1 2 0 – – – 0 1 2 2 2 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 – – – 1 2 1 1 7 5
Bud Site 0 0 0 0 0 1 0 2 1 1 4 3 0 0 0 – – – – – – – – –
Cell Periphery 5 4 11 16 10 13 17 5 9 5 14 11 14 16 24 – – – 1 1 4 3 25 17
Cytoplasm 37 68 68 68 141 188 314 224 229 265 233 288 107 222 209 – – – 17 25 6 14 63 58
Endoplasmic Reticulum 94 110 236 70 70 74 15 18 4 5 3 2 87 73 121 – – – 124 162 314 249 516 396
Endosome 0 0 4 7 8 8 9 4 7 3 4 5 0 0 1 – – – 2 2 1 0 3 6
Golgi 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 – – – 1 2 1 2 3 3
Mitochondria 3 7 23 40 47 179 303 252 245 425 369 387 0 0 0 – – – 4 4 6 17 42 26
Nucleus 0 1 0 0 0 1 6 2 4 4 5 4 0 2 0 – – – 0 1 0 0 0 0
Nuclear Periphery 0 1 2 0 0 2 11 6 4 4 4 7 0 0 0 – – – 0 0 2 0 0 3
Nucleolus 0 0 0 0 0 0 0 2 0 3 3 1 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 1 1 3 0 1 0 0 0 0 2 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 1 1 2 0 1 4 3 0 1 0 – – – 1 0 0 0 0 0
Vac/Vac Membrane 3 4 23 19 39 50 61 42 38 41 19 36 5 5 15 – – – 1 10 3 8 23 29
Unique Cell Count 135 179 336 180 282 432 619 467 433 609 537 614 197 299 337 163 221 359 309 708 567
Labelled Cell Count 148 201 380 228 320 520 742 580 555 773 682 765 215 324 371 163 221 359 309 708 567


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.0 6.6 6.8 6.6 5.5 4.4 4.0 4.0 3.8 3.4 3.5 3.6 7.1 7.0 7.8 – – – 8.1 8.0 8.2
Std Deviation (1e-4) 1.0 0.8 1.0 1.7 1.0 1.0 1.0 0.9 0.9 0.9 0.7 0.6 0.7 0.8 0.8 – – – 1.3 1.2 1.2
Intensity Change (Log2) – – – -0.04 -0.31 -0.63 -0.76 -0.76 -0.83 -1.0 -0.97 -0.9 0.07 0.05 0.2 – – – 0.26 0.23 0.27


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -0.1 -2.5 0 0 0 0 0 0 0 0 -2.1 -2.7 – – –
Bud 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Cell Periphery 2.7 0.2 0 0 0 0 0 0 0 2.0 1.3 2.2 – – –
Cytoplasm 4.3 7.8 6.8 9.2 8.1 9.2 7.2 7.0 8.1 8.1 13.6 11.0 – – –
Endoplasmic Reticulum -6.9 -11.2 0 0 0 0 0 0 0 -5.9 -11.5 -8.9 – – –
Endosome 0 1.5 0 0 0 0 0 0 0 0 0 0 – – –
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Mitochondria 5.1 3.8 0 0 0 0 0 0 0 -3.8 -4.6 -4.9 – – –
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – – –
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Vacuole 1.5 2.9 2.2 1.6 1.1 1.0 -0.1 -2.2 -0.6 -2.2 -3.2 -1.3 – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.7901 3.7186 3.7429 3.4851 3.5127 3.7778 4.6904 5.2605 5.0143 4.5782 4.9779 5.028 4.5753 5.1377 4.8253 5.1253 5.662 4.6113
Actin 0.007 0.0014 0.0019 0.0052 0.0006 0.0006 0.0222 0.0002 0.0092 0.0078 0.0083 0.0118 0.076 0.0042 0.0207 0.0085 0.006 0.0064
Bud 0.0001 0.0001 0.0002 0.0002 0 0.0045 0.0003 0 0.0002 0.0002 0.0001 0.0001 0.0013 0.0001 0.0004 0.0001 0 0
Bud Neck 0.0004 0.0003 0.0004 0.0014 0.0003 0.007 0.0054 0.0001 0.0008 0.0009 0.0008 0.0025 0.0014 0.0008 0.0012 0.0078 0.0023 0.0081
Bud Periphery 0.0001 0.0002 0.0004 0.0001 0.0001 0.0013 0.0005 0 0.0005 0.0003 0.0001 0.0002 0.0034 0.0002 0.0006 0.0004 0.0001 0.0001
Bud Site 0.0007 0.0035 0.0037 0.0004 0.0001 0.0002 0.0031 0.0004 0.0076 0.0006 0.0002 0.0003 0.0021 0.0066 0.0027 0.0004 0.0001 0.0001
Cell Periphery 0.0016 0.0034 0.0016 0.0004 0.0008 0.0007 0.0028 0.0021 0.0036 0.0014 0.0007 0.0013 0.0046 0.0123 0.0029 0.0054 0.0017 0.0015
Cytoplasm 0.294 0.3432 0.293 0.2967 0.3166 0.345 0.1247 0.157 0.1643 0.1683 0.2657 0.2676 0.2026 0.2734 0.1569 0.2435 0.2803 0.2568
Cytoplasmic Foci 0.0067 0.0089 0.0168 0.0063 0.0035 0.0066 0.0051 0.0047 0.0096 0.0096 0.0065 0.0133 0.0185 0.023 0.0078 0.0057 0.0038 0.0051
Eisosomes 0.0001 0.0001 0.0001 0.0001 0 0.0001 0.0003 0 0.0003 0.0007 0.0001 0.0001 0.0009 0.0007 0.0003 0.0014 0.0001 0
Endoplasmic Reticulum 0.6195 0.5268 0.5807 0.5483 0.6366 0.5481 0.7482 0.7886 0.7083 0.6655 0.6073 0.6403 0.6149 0.5613 0.7207 0.6595 0.6654 0.6972
Endosome 0.0118 0.0269 0.0293 0.0159 0.0104 0.0112 0.0147 0.0121 0.0097 0.0418 0.0376 0.0133 0.0087 0.0124 0.0094 0.0076 0.0014 0.004
Golgi 0.0018 0.0025 0.0024 0.0026 0.0006 0.0007 0.0043 0.0006 0.0043 0.0174 0.0046 0.0063 0.0074 0.007 0.0046 0.003 0.0026 0.0011
Lipid Particles 0.0037 0.0093 0.0062 0.0012 0.0012 0.0014 0.0127 0.0111 0.0095 0.0161 0.0074 0.011 0.0121 0.0422 0.0324 0.0072 0.0013 0.0081
Mitochondria 0.0006 0.0005 0.0009 0.0011 0.0004 0.0011 0.0021 0.0002 0.0093 0.005 0.0017 0.0011 0.002 0.0013 0.0108 0.0043 0.0001 0.0002
None 0.029 0.0365 0.0371 0.1055 0.0184 0.0562 0.0377 0.0058 0.0399 0.0465 0.0336 0.0193 0.0247 0.0178 0.0196 0.0333 0.0313 0.0037
Nuclear Periphery 0.01 0.0222 0.0138 0.0079 0.0054 0.0069 0.0086 0.0057 0.0041 0.0066 0.011 0.0034 0.0071 0.0066 0.0043 0.0054 0.0017 0.0033
Nucleolus 0.0001 0.0001 0.0002 0.0001 0 0.0001 0.0001 0 0.0001 0.0006 0.0001 0.0001 0.0003 0.0001 0 0.0001 0 0
Nucleus 0.0024 0.0048 0.0032 0.0018 0.0017 0.0036 0.0013 0.0013 0.0009 0.0014 0.0018 0.0011 0.0013 0.0009 0.0005 0.0013 0.0003 0.0014
Peroxisomes 0.0049 0.0001 0.0005 0.0004 0 0.0001 0.0007 0 0.0103 0.0018 0.0013 0.0031 0.0048 0.0246 0.0009 0.0002 0.0001 0.0001
Punctate Nuclear 0.0009 0.001 0.0014 0.0005 0.0004 0.0009 0.0008 0.0003 0.0007 0.0028 0.0046 0.0005 0.0027 0.0004 0.0005 0.0003 0.0001 0.0002
Vacuole 0.0041 0.0075 0.0056 0.0035 0.0026 0.003 0.0039 0.0095 0.0061 0.0036 0.0055 0.003 0.0024 0.0042 0.0023 0.0039 0.0012 0.0024
Vacuole Periphery 0.0004 0.0008 0.0006 0.0005 0.0002 0.0004 0.0006 0.0003 0.0009 0.0011 0.0012 0.0003 0.0008 0.0002 0.0003 0.0005 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 23.899 22.4506 15.5845 26.8207 28.3602 20.2422 28.3747 27.4292 18.4455 31.5709
Translational Efficiency 0.667 0.8353 0.8456 0.3795 0.4458 0.7326 0.7078 0.5493 0.71 0.5445

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
153 1279 427 1262 1853 1614 2509 1703 2006 2893 2936 2965

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 891.57 847.86 1092.87 1016.02 778.73 858.33 1046.31 1004.22 787.34 853.70 1053.08 1009.24
Standard Deviation 93.29 135.09 119.98 133.70 100.25 107.55 119.27 131.54 104.14 120.62 120.49 132.59
Intensity Change Log 2 — -0.072522 0.293702 0.188509 — 0.140409 0.426115 0.366880 — 0.030671 0.356951 0.274419

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000938 0.000721 0.002529 0.006462 0.000243 0.000596 0.001494 0.003163 0.000296 0.000651 0.001645 0.004567
Bud Neck 0.007170 0.002595 0.004431 0.011568 0.003081 0.003240 0.005478 0.010247 0.003393 0.002955 0.005325 0.010809
Bud Site 0.008657 0.008374 0.016457 0.038929 0.003508 0.003892 0.010704 0.037438 0.003900 0.005874 0.011541 0.038073
Cell Periphery 0.000727 0.001553 0.001393 0.003622 0.001752 0.001264 0.001513 0.001558 0.001674 0.001392 0.001495 0.002436
Cytoplasm 0.218233* 0.205177* 0.229265* 0.251477* 0.238698* 0.191112* 0.247905* 0.364372* 0.237137* 0.197330* 0.245194* 0.316320*
Cytoplasmic Foci 0.127738 0.075663 0.046508 0.066914 0.040214 0.052032 0.011908 0.060934 0.046890 0.062479 0.016940 0.063479
Eisosomes 0.000559 0.000486 0.000138 0.001589 0.001284 0.000788 0.000476 0.000787 0.001228 0.000655 0.000427 0.001128
Endoplasmic Reticulum 0.148780* 0.533207* 0.405905* 0.242612* 0.557567* 0.578505* 0.530516* 0.182525* 0.526388* 0.558478* 0.512393* 0.208100*
Endosome 0.031163 0.013519 0.019328 0.015038 0.005170 0.012091 0.005645 0.012863 0.007153 0.012722 0.007635 0.013789
Golgi 0.004546 0.020549 0.001947 0.004884 0.022415 0.022091 0.001507 0.002452 0.021052 0.021409 0.001571 0.003487
Lipid Particles 0.061652 0.050243 0.026871 0.039277 0.056232 0.059859 0.014383 0.025152 0.056645 0.055608 0.016199 0.031164
Mitochondria 0.010330 0.008531 0.000161 0.000878 0.014251 0.013403 0.001101 0.000834 0.013952 0.011249 0.000965 0.000853
Mitotic Spindle 0.002263 0.002181 0.000950 0.006090 0.000049 0.000777 0.000857 0.004061 0.000218 0.001398 0.000871 0.004925
None 0.000325 0.006524 0.000308 0.001736 0.013475 0.004072 0.001720 0.003098 0.012472 0.005156 0.001515 0.002519
Nuclear Periphery 0.008445 0.006384 0.005519 0.003592 0.001696 0.004042 0.005692 0.003645 0.002211 0.005077 0.005667 0.003622
Nuclear Periphery Foci 0.024814 0.011389 0.115607 0.032542 0.002460 0.005830 0.025508 0.024387 0.004165 0.008288 0.038612 0.027858
Nucleolus 0.000785 0.000594 0.000079 0.000310 0.000285 0.000392 0.000153 0.000671 0.000323 0.000481 0.000142 0.000517
Nucleus 0.057386 0.022147 0.030419 0.052339 0.024227 0.018956 0.084184 0.086002 0.026756 0.020367 0.076365 0.071674
Peroxisomes 0.005361 0.003031 0.000513 0.002894 0.004626 0.003483 0.000513 0.001980 0.004682 0.003283 0.000513 0.002369
Vacuole 0.277690* 0.023772 0.091252 0.216490* 0.007322 0.020127 0.048539 0.172955 0.027944 0.021738 0.054751 0.191485
Vacuole Periphery 0.002435 0.003359 0.000420 0.000756 0.001444 0.003448 0.000204 0.000876 0.001520 0.003409 0.000235 0.000825

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.54 -2.79 -6.87 -8.02 -4.38 -2.31 -4.26 -7.87 -6.38 -3.15 -3.58 -5.14 -11.49 -10.22 -5.97
Bud Neck 2.45 1.33 -2.23 -9.61 -6.86 -0.18 -2.98 -8.42 -7.62 -5.29 0.61 -2.69 -9.82 -11.96 -7.23
Bud Site 0.10 -1.83 -6.36 -9.10 -4.92 -0.44 -5.28 -11.20 -11.08 -7.89 -2.05 -5.98 -15.30 -14.27 -10.37
Cell Periphery -1.70 -3.67 -5.62 -2.64 -3.97 2.08 1.30 1.91 -0.60 0.72 1.04 1.04 -2.18 -2.59 -3.11
Cytoplasm 0.64 -0.59 -1.55 -4.69 -1.24 6.54 -1.56 -14.79 -20.02 -14.02 6.35 -1.46 -11.08 -18.31 -10.32
Cytoplasmic Foci 4.72 7.21 5.51 1.81 -4.63 -3.69 12.71 -6.62 -2.48 -21.01 -5.17 12.85 -5.88 -0.30 -24.17
Eisosomes 0.56 4.07 -4.64 -5.46 -7.72 3.80 6.98 3.69 0.67 -1.78 5.16 7.52 1.50 -3.34 -5.67
Endoplasmic Reticulum -16.04 -9.61 -3.10 23.71 10.69 -1.72 3.41 39.15* 38.03* 38.50* -3.08 2.21 36.36 43.91* 39.23*
Endosome 5.10 3.33 4.82 -0.95 3.55 -7.61 -1.26 -9.21 0.12 -8.42 -7.55 -1.16 -9.36 -0.48 -9.10
Golgi -7.98 1.49 0.02 10.74 -2.84 0.19 19.04 18.27 15.34 -2.61 -0.28 18.96 16.60 18.67 -3.91
Lipid Particles 1.13 3.84 2.62 4.01 -3.56 -1.04 16.03 11.98 13.55 -7.19 0.28 16.07 10.29 12.82 -10.97
Mitochondria 0.25 2.00 1.86 7.76 -4.45 0.46 13.00 13.44 8.40 1.80 1.92 12.92 13.17 11.03 1.04
Mitotic Spindle -0.03 0.87 -2.17 -2.50 -4.02 -2.28 -2.43 -4.40 -3.59 -3.31 -2.59 -2.25 -6.22 -4.24 -4.92
None -4.84 0.09 -1.95 3.38 -2.00 5.56 8.20 6.79 1.04 -1.61 4.83 8.28 7.29 3.09 -1.59
Nuclear Periphery 0.67 1.06 1.86 3.24 2.73 -4.89 -8.97 -4.72 0.89 4.10 -5.77 -7.91 -3.63 3.10 4.86
Nuclear Periphery Foci 3.31 -12.37 -1.79 -9.37 12.69 -5.17 -19.99 -15.19 -11.96 0.59 -5.80 -23.00 -19.50 -14.89 5.87
Nucleolus 0.45 2.19 1.35 1.79 -5.31 -1.09 3.77 -2.69 -1.87 -3.43 -1.87 4.50 -2.41 -0.73 -4.19
Nucleus 4.39 3.13 0.51 -11.27 -6.17 3.24 -21.56 -21.23 -23.26 -1.93 4.14 -19.55 -20.41 -25.28 0.44
Peroxisomes 0.89 1.92 0.97 0.51 -7.15 2.73 14.20 8.46 4.83 -8.30 3.60 12.73 6.62 3.93 -10.66
Vacuole 12.01 8.38 1.54 -30.85 -16.44 -9.13 -22.23 -37.54 -34.14 -26.96 2.64 -9.99 -39.61 -45.68 -34.80
Vacuole Periphery -0.82 1.55 1.31 6.67 -1.53 -4.24 12.42 3.80 5.51 -3.67 -5.76 9.75 4.52 8.26 -4.29
Standard name
Human Ortholog
Description Protein involved in ER-to-Golgi transport
Localization
Cell Percentages ER (94%)
Cell Cycle Regulation No
Subcompartmental Group ER-3

Sly41

Sly41


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sly41-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available