Standard name
Human Ortholog
Description Ribosomal 60S subunit protein L20B; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20B has a paralog, RPL20A, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 – 0 0 0 0 0 0 0.05 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.0 0.89 – 0.99 0.97 0.96 0.87 0.8 0.82 0.84 0.76 0.76 0.97 0.99 0.99 0.99 0.96 0.97 0.91 0.87 0.84 0.82 0.88 0.82
Endoplasmic Reticulum 0 0.06 – 0 0 0 0.11 0.17 0.19 0.12 0.19 0.16 0 0 0 0 0 0 0.07 0 0 0.07 0.08 0.13
Endosome 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.06 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0.06 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0.06 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 1 2 2 1 2 2 7 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0
Bud Neck 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 – 0 4 3 8 8 6 8 3 9 1 1 3 2 4 6 0 0 0 0 0 0
Cytoplasm 81 16 – 144 286 170 222 222 136 123 103 151 154 179 192 230 200 248 71 40 20 61 135 164
Endoplasmic Reticulum 0 1 – 3 10 4 29 48 31 17 25 31 7 0 1 5 7 9 5 1 0 5 12 25
Endosome 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 – 0 0 1 1 1 2 1 1 5 0 0 2 0 1 2 0 0 0 0 0 1
Mitochondria 0 1 – 0 1 0 0 0 1 0 4 1 0 0 0 0 2 0 0 0 0 0 0 0
Nucleus 0 1 – 0 0 0 1 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 1 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 – 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Vac/Vac Membrane 0 0 – 0 0 1 0 3 0 2 1 3 0 2 0 1 3 1 0 1 0 4 0 3
Unique Cell Count 81 18 146 295 177 255 277 165 147 135 198 159 181 193 233 208 256 79 47 24 75 154 200
Labelled Cell Count 81 20 147 301 180 263 285 177 153 140 208 162 182 198 238 217 268 79 47 24 75 154 200


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 93.7 77.3 – 87.1 70.0 56.5 50.0 47.8 45.3 41.3 40.6 40.4 90.9 91.5 90.3 142.8 145.7 143.2 86.6 88.2 95.7
Std Deviation (1e-4) 16.0 27.4 – 17.0 13.9 11.6 11.5 11.7 10.6 10.2 11.1 10.8 20.3 22.0 20.6 39.0 39.1 42.7 14.9 20.8 23.9
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 62.1388 110.7212 96.9045 62.7445 87.1795 87.6773 99.8048 124.3057 111.2874 122.818 107.5973 102.0187 96.7663 131.3147 114.8711 121.2984 32.6527 121.3378
Actin 0.025 0.0004 0.0017 0.0043 0.0033 0.0017 0.0054 0.0007 0.0032 0.0005 0.003 0.002 0.0106 0.0007 0.0026 0.001 0.0446 0.0047
Bud 0.0074 0.0007 0.002 0.049 0.0008 0.0034 0.0027 0.0013 0.0034 0.0018 0.0067 0.0013 0.0035 0.0022 0.0073 0.003 0.0487 0.0116
Bud Neck 0.0014 0.0005 0.0005 0.0018 0.0013 0.0032 0.0007 0.0006 0.0011 0.0003 0.0017 0.0018 0.0011 0.0015 0.0013 0.0019 0.0016 0.0011
Bud Periphery 0.0177 0.0012 0.0013 0.0711 0.0019 0.0027 0.0035 0.0008 0.0034 0.0005 0.0041 0.0013 0.0038 0.0027 0.0063 0.0016 0.0724 0.0056
Bud Site 0.0025 0.0006 0.001 0.0093 0.0002 0.0008 0.0011 0.0013 0.0016 0.0003 0.001 0.0003 0.0016 0.0018 0.0043 0.0011 0.0208 0.0006
Cell Periphery 0.0339 0.0206 0.0092 0.0114 0.0154 0.0068 0.0313 0.0133 0.0173 0.0325 0.0089 0.0209 0.0219 0.0492 0.0343 0.088 0.0039 0.0028
Cytoplasm 0.4419 0.8839 0.799 0.469 0.4924 0.6457 0.6201 0.8691 0.7969 0.69 0.5979 0.5984 0.5832 0.7162 0.6683 0.4018 0.2397 0.7758
Cytoplasmic Foci 0.0091 0.0015 0.0029 0.0037 0.004 0.0038 0.0017 0.002 0.0047 0.001 0.0017 0.0084 0.0251 0.0024 0.015 0.0053 0.0363 0.0054
Eisosomes 0.0007 0.0002 0 0 0.0061 0.0001 0.0001 0.0001 0.0007 0 0.0001 0.0003 0.0003 0.0001 0.0004 0.0001 0.0001 0
Endoplasmic Reticulum 0.0994 0.0112 0.0065 0.0122 0.1572 0.1136 0.0633 0.0179 0.0093 0.0006 0.005 0.0759 0.0564 0.0245 0.0242 0.006 0.0142 0.0184
Endosome 0.0385 0.0032 0.0093 0.018 0.0577 0.0271 0.0155 0.0043 0.0152 0.0033 0.0135 0.0665 0.0166 0.0079 0.0152 0.023 0.1944 0.0303
Golgi 0.0084 0.0007 0.0016 0.002 0.049 0.0027 0.0038 0.0013 0.0035 0.0001 0.0027 0.0265 0.0045 0.0015 0.0024 0.0011 0.0118 0.0064
Lipid Particles 0.006 0.0011 0.0009 0.0011 0.0004 0.0023 0.0012 0.0008 0.0039 0.0004 0.0006 0.0033 0.0062 0.0024 0.0059 0.0046 0.0015 0.0004
Mitochondria 0.0452 0.0043 0.0012 0.0104 0.1555 0.0047 0.0128 0.0166 0.0055 0.0001 0.0551 0.0654 0.0088 0.0012 0.008 0.001 0.011 0.0108
None 0.003 0.0005 0.0004 0.0007 0.0008 0.0029 0.0011 0.0003 0.0025 0.0001 0.0005 0.0011 0.0121 0.0003 0.0143 0.0006 0.0087 0.0009
Nuclear Periphery 0.0977 0.0052 0.0058 0.0135 0.0082 0.0271 0.0528 0.0034 0.012 0.0028 0.0152 0.0187 0.0373 0.0063 0.0135 0.0123 0.0207 0.0131
Nucleolus 0.0007 0.0021 0.0006 0.0017 0.0001 0.0078 0.0004 0.0012 0.0008 0.001 0.0005 0.0052 0.0021 0.0012 0.0029 0.0019 0.001 0.0003
Nucleus 0.0159 0.0042 0.0031 0.0105 0.0012 0.0268 0.0095 0.0022 0.0048 0.0046 0.0065 0.0112 0.0095 0.0038 0.0056 0.0059 0.0126 0.0067
Peroxisomes 0.0068 0.0001 0.0001 0.0007 0 0.0003 0.0001 0.0002 0.0002 0 0.0002 0.0002 0.0049 0.0001 0.0008 0.0003 0.0016 0.0002
Punctate Nuclear 0.0026 0 0.0001 0.0002 0 0.0025 0.0002 0 0.0003 0 0.0001 0.0002 0.0011 0 0.0007 0.0001 0.0019 0.0003
Vacuole 0.0967 0.0511 0.1225 0.2284 0.0264 0.0872 0.144 0.057 0.0874 0.2339 0.1849 0.0471 0.1448 0.1517 0.1324 0.3457 0.1886 0.0587
Vacuole Periphery 0.0395 0.0067 0.0302 0.0811 0.0181 0.0268 0.0287 0.0056 0.0222 0.0259 0.09 0.044 0.0447 0.0223 0.0344 0.0938 0.0638 0.046

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 1848.5745 1853.0188 1960.2848 1998.8584 1922.6434 1776.3689 1722.7378 1586.192 1546.622 1651.4789
Translational Efficiency 1.7235 1.8088 1.7999 1.3608 1.8641 1.8813 1.6619 1.7863 1.7929 2.0399

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1370 572 2083 267 1709 20 2093 619 3079 592 4176 886

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 2570.45 3880.72 8008.16 3925.31 3406.84 298.96 6255.69 7207.92 3034.69 3759.71 7129.83 6218.69
Standard Deviation 1274.46 1591.67 1827.89 1351.20 1181.03 7.97 2364.28 1677.97 1292.16 1693.11 2288.23 2187.69
Intensity Change Log 2 — 0.594303 1.639450 0.610786 — -3.510410 0.876735 1.081149 — -0.516099 1.254800 0.897309

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000159 0.000176 0.000224 0.000202 0.000107 0.000022 0.000164 0.000226 0.000130 0.000170 0.000194 0.000219
Bud Neck 0.001157 0.001174 0.000645 0.024030 0.000534 0.000012 0.002232 0.002130 0.000811 0.001135 0.001441 0.008730
Bud Site 0.017532 0.008268 0.007452 0.017570 0.010583 0.000040 0.011556 0.009333 0.013675 0.007990 0.009509 0.011815
Cell Periphery 0.010152 0.028930 0.111673* 0.010214 0.016680 0.000102 0.027984 0.109463* 0.013775 0.027956 0.069728 0.079554
Cytoplasm 0.733161* 0.504790* 0.279040* 0.243810* 0.611767* 0.000048 0.458905* 0.201434* 0.665781* 0.487738* 0.369188* 0.214204*
Cytoplasmic Foci 0.040923 0.022157 0.008732 0.205456* 0.021709 0.000014 0.016649 0.006182 0.030258 0.021409 0.012700 0.066234
Eisosomes 0.000002 0.000003 0.000008 0.000009 0.000002 0.000030 0.000004 0.000008 0.000002 0.000004 0.000006 0.000008
Endoplasmic Reticulum 0.001626 0.002320 0.009321 0.000221 0.004989 0.000001 0.002019 0.000946 0.003492 0.002241 0.005661 0.000727
Endosome 0.002236 0.002028 0.001513 0.004199 0.002271 0.000001 0.002453 0.001057 0.002255 0.001960 0.001984 0.002004
Golgi 0.057758 0.081244 0.057455 0.016312 0.078543 0.000006 0.029893 0.032802 0.069295 0.078499 0.043641 0.027833
Lipid Particles 0.007934 0.032423 0.041444 0.011123 0.012904 0.000021 0.026360 0.059246 0.010692 0.031328 0.033884 0.044744
Mitochondria 0.000754 0.000196 0.000734 0.000840 0.000809 0.000017 0.000507 0.000159 0.000784 0.000190 0.000621 0.000364
Mitotic Spindle 0.003930 0.005234 0.000891 0.158389* 0.002371 0.000266 0.014994 0.002757 0.003065 0.005066 0.007959 0.049657
None 0.001018 0.001839 0.000816 0.004288 0.001944 0.999258* 0.001497 0.000503 0.001532 0.035535 0.001157 0.001644
Nuclear Periphery 0.000287 0.000786 0.001638 0.006187 0.000440 0.000000 0.001293 0.002778 0.000372 0.000760 0.001465 0.003805
Nuclear Periphery Foci 0.006492 0.026681 0.008411 0.059434 0.010512 0.000038 0.020227 0.010984 0.008723 0.025781 0.014333 0.025585
Nucleolus 0.000067 0.000175 0.000178 0.016767 0.000099 0.000039 0.000241 0.000168 0.000085 0.000170 0.000209 0.005170
Nucleus 0.008563 0.008425 0.002552 0.075890 0.004147 0.000014 0.012586 0.006990 0.006112 0.008141 0.007581 0.027753
Peroxisomes 0.000094 0.000058 0.000423 0.002559 0.000136 0.000067 0.000092 0.000059 0.000118 0.000058 0.000257 0.000812
Vacuole 0.042669 0.162798 0.281339* 0.112649 0.083164 0.000001 0.288467* 0.485480* 0.065146 0.157298 0.284911* 0.373126*
Vacuole Periphery 0.063486 0.110295* 0.185512* 0.029853 0.136289* 0.000000 0.081875 0.067295 0.103895* 0.106569* 0.133570* 0.056011

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.49 -3.11 -5.10 -2.13 -2.30 7.65 -4.00 -4.25 -6.78 -1.36 -0.97 -5.21 -5.45 -1.72 -1.95
Bud Neck 0.38 2.49 -10.92 -10.95 -11.07 12.20 -10.90 -7.23 -8.72 1.35 -1.89 -7.50 -10.68 -10.47 -9.68
Bud Site 4.74 6.43 -4.24 -7.89 -9.20 10.04 -2.88 0.09 -6.10 2.20 3.51 2.60 -1.38 -4.08 -3.31
Cell Periphery -6.35 -32.87* 2.88 7.55 32.46* 16.31 -4.57 -14.65 -18.10 -13.17 -5.03 -28.84 -13.52 -8.46 -0.18
Cytoplasm 13.18 42.00 32.42 15.72 6.87 71.72* 14.16 27.22 -19.75 16.28 10.94 37.16 41.39 15.82 14.82
Cytoplasmic Foci 4.99 14.13 -12.32 -13.29 -14.33 19.17 -2.41 6.15 -7.13 6.85 2.51 8.01 -8.14 -8.71 -10.10
Eisosomes -2.52 -9.09 -7.41 -6.50 -3.52 -3.39 -5.80 -6.21 2.60 -4.46 -3.60 -10.57 -9.38 -6.02 -4.53
Endoplasmic Reticulum -1.25 -13.15 5.49 4.45 17.20 9.86 6.06 7.96 -7.63 4.27 2.43 -4.80 8.97 3.40 16.47
Endosome 0.89 4.37 -6.42 -6.85 -8.47 18.75* -2.61 7.38 -12.49 8.98 1.62 0.81 -0.49 -1.76 -1.10
Golgi -4.49 -0.04 14.51 14.97 19.49* 26.96* 16.54 12.76 -12.63 -1.01 -1.78 11.47 16.34 11.45 8.45
Lipid Particles -7.69 -20.74 -1.85 5.68 12.34 14.38 -8.18 -12.09 -16.26 -8.23 -6.75 -20.06 -12.11 -2.83 -3.36
Mitochondria 1.26 0.04 -0.80 -6.96 -2.70 7.80 1.78 5.65 -4.77 6.94 2.92 0.54 1.49 -5.27 3.27
Mitotic Spindle -0.98 5.16 -8.81 -8.64 -9.01 6.81 -9.19 -1.97 -3.62 6.57 -1.39 -7.44 -8.24 -7.37 -6.66
None -4.24 2.31 -7.13 -5.13 -7.65 -4082.04* 1.16 6.29 6569.93* 6.34 -4.58 2.14 -0.82 4.56 -2.67
Nuclear Periphery -6.10 -19.74 -6.48 -5.94 -5.07 15.59* -11.37 -1.64 -1.97 -0.87 -5.02 -20.73 -3.49 -3.09 -2.29
Nuclear Periphery Foci -8.48 -3.11 -6.25 -3.03 -5.96 17.94* -8.46 -1.78 -16.11 6.88 -7.45 -8.59 -6.58 0.89 -3.35
Nucleolus -4.34 -17.06 -6.83 -6.81 -6.81 6.37 -8.55 -3.81 -6.11 3.91 -3.61 -11.90 -6.51 -6.45 -6.39
Nucleus 0.60 6.99 -8.51 -8.58 -9.19 8.82 -10.84 -4.69 -7.06 2.24 -1.82 -5.47 -8.87 -8.13 -7.51
Peroxisomes 2.16 -6.91 -3.72 -3.75 -3.43 2.26 1.17 3.03 0.54 3.39 3.34 -5.16 -3.51 -3.69 -3.09
Vacuole -13.49 -42.98* -5.65 4.75 16.35 26.14* -29.73 -33.36 -42.35 -14.94 -10.73 -49.38 -28.43 -15.36 -7.09
Vacuole Periphery -5.78 -23.00* 9.25 12.04 34.32* 31.08* 13.12 13.77 -17.87 2.85 -0.12 -6.37 14.06 7.97 23.04*
Standard name
Human Ortholog
Description Ribosomal 60S subunit protein L20B; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20B has a paralog, RPL20A, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-2

Rpl20b

Rpl20b


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rpl20b-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available