Standard name
Human Ortholog
Description Long chain fatty acyl-CoA synthetase; activates fatty acids with a preference for C12:0-C16:0 chain lengths; role in the competitive import of long-chain fatty acids and sphingoid long-chain bases; accounts for most acyl-CoA synthetase activity; localizes to lipid particles and the plasma membrane; role in sphingolipid-to-glycerolipid metabolism; forms ER foci upon replication stress; faa1 faa4 double null complemented by any of human ACSBG1, ACSL1, 3, 4, 5, 6, SLC27A2, or 4
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.1 0 0.07 0 0.07 0 0 0 0 0 0 0 0.08 0.08 0.08 0.08 0 0.07 0 0 0 0 0 0
Bud 0 0.08 0 0 0 0 0 0.05 0.06 0.06 0.08 0 0.05 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.29 0.18 0.52 0.57 0.6 0.44 0.42 0.32 0.38 0.3 0.25 0.33 0.76 0.78 0.79 0.68 0.65 0.62 0.05 0 0.11 0.07 0.16 0.16
Cytoplasm 0.3 0.58 0.3 0.35 0.31 0.5 0.5 0.51 0.69 0.63 0.61 0.61 0.26 0.26 0.25 0.34 0.33 0.31 0.08 0.31 0.17 0.15 0.09 0.08
Endoplasmic Reticulum 0.49 0.3 0.39 0.38 0.3 0.33 0.33 0.32 0.17 0.2 0.2 0.28 0.19 0.23 0.16 0 0 0 0.81 0.57 0.66 0.72 0.69 0.7
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 20 4 25 18 28 11 24 14 16 19 11 10 27 33 37 10 2 7 5 2 4 0 9 4
Bud 4 13 14 5 11 8 11 33 22 26 31 15 18 7 7 1 2 3 1 0 3 0 3 4
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 6 4 2 3 4
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 58 28 174 250 242 198 240 195 139 129 99 127 251 338 380 88 77 62 10 7 46 13 68 81
Cytoplasm 61 88 100 154 124 222 281 309 256 269 242 236 85 111 118 44 39 31 18 60 72 28 38 39
Endoplasmic Reticulum 100 46 129 164 119 146 184 197 64 86 79 110 62 100 77 1 4 4 184 112 277 142 304 353
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Golgi 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 2 4 2 0 0 0 0 1 2
Mitochondria 0 1 0 0 1 0 0 0 2 2 6 0 0 0 0 1 0 0 0 1 5 1 2 5
Nucleus 0 0 0 0 1 0 0 2 0 1 2 0 1 0 1 1 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 3 2 0 0 0 0 0 0 0 1 0 0 1 0 1
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 1 0 2 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 4 0 9 15 3 0 0 1 1 1 2 2 2 5 1 2 4
Unique Cell Count 203 153 335 436 402 448 566 611 369 426 395 386 332 434 478 129 119 100 229 198 422 197 439 508
Labelled Cell Count 243 180 443 592 526 585 740 754 499 544 487 501 444 589 622 150 129 113 229 198 422 197 439 508


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 21.9 20.2 25.1 21.7 22.4 21.0 20.0 21.4 19.7 18.5 17.2 20.5 23.5 26.6 26.8 30.3 30.4 29.8 22.5 23.3 24.2
Std Deviation (1e-4) 5.2 4.6 5.4 3.6 4.4 4.4 4.2 4.3 4.2 4.4 4.2 5.1 5.3 6.8 7.6 9.3 9.4 7.7 4.7 5.9 5.5
Intensity Change (Log2) – – – -0.21 -0.16 -0.26 -0.33 -0.23 -0.35 -0.44 -0.54 -0.29 -0.09 0.08 0.09 0.27 0.28 0.25 -0.15 -0.1 -0.05


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -2.0 -0.3 -3.3 -2.1 -3.8 -1.8 -1.8 -2.9 -3.0 0.3 0.1 0.1 0.1 -2.3 -0.2
Bud -2.7 -1.1 -2.0 -2.0 0.8 1.1 1.2 2.1 -0.2 0.8 -2.2 -2.4 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1.5 2.3 -2.1 -2.8 -6.0 -3.8 -6.1 -7.5 -5.2 6.4 7.6 8.3 3.2 2.4 1.8
Cytoplasm 1.6 0.3 5.5 5.8 6.2 10.5 9.1 8.5 8.4 -1.2 -1.3 -1.6 0.9 0.6 0.2
Endoplasmic Reticulum -0.3 -2.5 -1.7 -1.8 -1.9 -6.3 -5.6 -5.5 -2.8 -5.7 -4.7 -7.2 -8.1 -7.2 -6.6
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 3.6 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 31.367 33.9957 32.4165 34.4882 30.9165 34.3222 45.6454 51.0226 47.5934 42.829 36.7373 45.9897 53.8104 57.5399 54.5025 48.0506 48.3153 62.7306
Actin 0.0078 0.0004 0.003 0 0.0056 0.0007 0.0123 0.0104 0.0029 0.0002 0.0037 0.002 0.0057 0.0014 0.002 0.0224 0.0907 0.0005
Bud 0.0022 0.0001 0.0006 0 0.0008 0.0001 0.0018 0.0001 0.0011 0.0002 0.0012 0.0003 0.0009 0.0001 0.0002 0.0009 0.0004 0.0002
Bud Neck 0.0006 0.0005 0.0013 0.0006 0.0009 0.003 0.0008 0.0002 0.0005 0.0048 0.0028 0.0032 0.0014 0.0035 0.0031 0.0045 0.0026 0.0086
Bud Periphery 0.0104 0.0007 0.0038 0.0007 0.0049 0.0013 0.0095 0.0005 0.0034 0.0042 0.0035 0.0026 0.0046 0.001 0.0019 0.0058 0.0013 0.0032
Bud Site 0.0018 0.0021 0.0021 0 0.0012 0.0001 0.0029 0.001 0.003 0.0002 0.0248 0.0002 0.0024 0.0031 0.0007 0.0014 0.0008 0.0001
Cell Periphery 0.1944 0.2384 0.2423 0.1764 0.2627 0.143 0.3266 0.3099 0.3477 0.3838 0.2646 0.1916 0.2398 0.3112 0.2902 0.3101 0.1116 0.2048
Cytoplasm 0.0441 0.0844 0.0336 0.0077 0.0348 0.0263 0.0159 0.0164 0.0101 0.0054 0.0079 0.0143 0.0693 0.0641 0.0326 0.0302 0.0116 0.0135
Cytoplasmic Foci 0.007 0.0005 0.0063 0 0.0122 0.0003 0.0019 0.0001 0.004 0 0.0087 0.0029 0.0057 0.001 0.0047 0.0152 0.0067 0.0001
Eisosomes 0.0009 0.0015 0.0008 0.0001 0.0006 0.0002 0.0016 0.0008 0.0005 0.0017 0.0003 0.0003 0.0008 0.0005 0.0019 0.0005 0.0005 0.0007
Endoplasmic Reticulum 0.6348 0.5759 0.6158 0.7785 0.4681 0.7911 0.5305 0.5888 0.5438 0.5204 0.5322 0.7452 0.5104 0.5058 0.5406 0.4139 0.6643 0.7043
Endosome 0.0071 0.0006 0.0019 0.0001 0.0403 0.0005 0.0057 0.0001 0.0014 0 0.0045 0.0008 0.0119 0.0002 0.0147 0.0139 0.0388 0.0001
Golgi 0.0021 0.0007 0.0022 0.0001 0.043 0.0004 0.0033 0.0004 0.0013 0.0002 0.0047 0.0016 0.0043 0.0006 0.002 0.0134 0.0133 0.0006
Lipid Particles 0.0119 0.0574 0.0254 0.009 0.0421 0.0142 0.0068 0.0119 0.0066 0.0035 0.0487 0.0055 0.0154 0.0449 0.016 0.0327 0.0122 0.0123
Mitochondria 0.0088 0.0037 0.0079 0 0.038 0.0002 0.0043 0.0002 0.0031 0 0.0553 0.0027 0.01 0 0.0003 0.0167 0.0031 0
None 0.003 0.0003 0.001 0 0.0009 0.0002 0.0006 0 0.0006 0 0.0001 0.0001 0.0011 0.0001 0.0002 0.0022 0.0022 0
Nuclear Periphery 0.0228 0.012 0.0153 0.0105 0.0125 0.0075 0.0399 0.0329 0.0391 0.0449 0.0167 0.0119 0.0676 0.0338 0.0458 0.0437 0.0225 0.0296
Nucleolus 0.001 0.0002 0.0006 0 0.0004 0.0001 0.0002 0.0001 0.0002 0.0001 0.0003 0.0002 0.0005 0.0001 0.0001 0.0023 0.0002 0.0001
Nucleus 0.0026 0.0004 0.0015 0.0002 0.001 0.0004 0.001 0.0002 0.0008 0.0004 0.0003 0.0004 0.0043 0.0006 0.0007 0.0014 0.0012 0.0007
Peroxisomes 0.0005 0 0.0054 0 0.0048 0 0.0007 0 0.0006 0 0.006 0.0007 0.0016 0 0.0053 0.0122 0.006 0
Punctate Nuclear 0.0026 0.0001 0.0006 0 0.0005 0 0.0005 0 0.0001 0 0.0003 0 0.0004 0.0001 0.0003 0.002 0.0036 0
Vacuole 0.0315 0.0193 0.0272 0.0157 0.0182 0.01 0.0317 0.0258 0.0281 0.0291 0.0131 0.0129 0.0389 0.0275 0.0357 0.0499 0.0048 0.0202
Vacuole Periphery 0.0018 0.0009 0.0013 0.0002 0.0066 0.0002 0.0015 0.0002 0.001 0.0006 0.0004 0.0006 0.0032 0.0002 0.0009 0.0046 0.0015 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 54.3045 30.9562 32.716 60.3837 68.0272 62.4025 43.2743 43.2107 53.131 68.7882
Translational Efficiency 1.8731 2.4067 1.7957 1.5223 1.6543 1.6868 1.3888 1.3295 1.6198 1.316

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
593 1201 889 1149 1123 1581 1197 2251 1716 2782 2086 3400

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1589.66 1668.14 4376.84 4181.24 1585.86 1711.49 3684.63 4115.46 1587.17 1692.78 3979.63 4137.69
Standard Deviation 300.65 419.22 918.20 849.66 379.68 403.17 738.88 1052.30 354.37 410.74 888.69 988.97
Intensity Change Log 2 — 0.069522 1.461171 1.395213 — 0.109987 1.216254 1.375788 — 0.089872 1.344050 1.385545

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000610 0.003989 0.000692 0.000586 0.001606 0.001223 0.000681 0.000944 0.001262 0.002417 0.000686 0.000823
Bud Neck 0.003370 0.005226 0.001886 0.005106 0.005967 0.002901 0.002381 0.006984 0.005070 0.003905 0.002170 0.006350
Bud Site 0.010354 0.046236 0.002827 0.011727 0.022930 0.014893 0.005874 0.025223 0.018584 0.028424 0.004576 0.020662
Cell Periphery 0.293242* 0.223705* 0.574382* 0.359562* 0.353032* 0.187643* 0.345853* 0.256182* 0.332370* 0.203211* 0.443246* 0.291119*
Cytoplasm 0.044310 0.102624* 0.001531 0.026927 0.113927* 0.064662 0.020221 0.123665* 0.089869 0.081050 0.012256 0.090973
Cytoplasmic Foci 0.003128 0.007203 0.000103 0.001804 0.005214 0.006531 0.000542 0.010858 0.004493 0.006821 0.000355 0.007798
Eisosomes 0.000353 0.001156 0.001055 0.000217 0.000864 0.000460 0.000430 0.000182 0.000687 0.000760 0.000696 0.000194
Endoplasmic Reticulum 0.506700* 0.457750* 0.028046 0.102424* 0.338313* 0.550283* 0.218258* 0.136425* 0.396503* 0.510336* 0.137195* 0.124935*
Endosome 0.005231 0.007215 0.000681 0.003278 0.004676 0.007950 0.002987 0.010107 0.004868 0.007633 0.002004 0.007799
Golgi 0.006111 0.006007 0.004287 0.008762 0.004804 0.007524 0.005577 0.007319 0.005255 0.006869 0.005027 0.007807
Lipid Particles 0.054485 0.068519 0.013302 0.205070* 0.052135 0.084696 0.114399* 0.175764* 0.052947 0.077712 0.071314 0.185668*
Mitochondria 0.000545 0.001008 0.000165 0.000380 0.000825 0.001567 0.000242 0.001159 0.000728 0.001326 0.000209 0.000896
Mitotic Spindle 0.000804 0.001396 0.001240 0.001581 0.000980 0.000651 0.000874 0.008884 0.000919 0.000973 0.001030 0.006416
None 0.000589 0.000844 0.000033 0.001287 0.000413 0.001033 0.000211 0.001702 0.000473 0.000951 0.000135 0.001562
Nuclear Periphery 0.005377 0.002563 0.023614 0.010544 0.006394 0.003913 0.013313 0.009978 0.006043 0.003330 0.017703 0.010170
Nuclear Periphery Foci 0.004698 0.004465 0.001871 0.006494 0.004297 0.004358 0.005210 0.011555 0.004435 0.004404 0.003787 0.009845
Nucleolus 0.000044 0.000062 0.000086 0.000077 0.000086 0.000076 0.000042 0.000204 0.000072 0.000070 0.000061 0.000162
Nucleus 0.000689 0.001503 0.000525 0.000972 0.001698 0.001303 0.000889 0.003702 0.001349 0.001390 0.000734 0.002780
Peroxisomes 0.004817 0.005237 0.000201 0.001393 0.007302 0.007146 0.000963 0.005272 0.006443 0.006322 0.000639 0.003961
Vacuole 0.050976 0.050169 0.335823* 0.246459* 0.072287 0.048008 0.258649* 0.198517 0.064923 0.048941 0.291538* 0.214718*
Vacuole Periphery 0.003566 0.003124 0.007651 0.005347 0.002250 0.003179 0.002403 0.005371 0.002705 0.003155 0.004639 0.005363

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -7.45 -0.39 -0.19 7.27 0.25 1.06 2.52 1.94 0.91 -0.94 -4.05 2.06 1.74 6.21 -0.63
Bud Neck -2.31 1.61 -1.99 -0.48 -3.16 3.07 3.60 -1.69 -6.24 -7.27 1.63 3.97 -2.48 -4.94 -7.58
Bud Site -8.27 3.15 -1.03 7.59 -4.53 2.88 6.35 -1.64 -4.92 -8.80 -3.97 7.34 -1.97 2.28 -10.24
Cell Periphery 5.67 -23.57* -5.16 -13.93 24.10* 17.46 0.94 11.94 -7.53 13.38 17.08 -13.74 6.88 -13.24 25.32*
Cytoplasm -8.60 9.47 3.29 13.25 -9.99 7.66 15.94 -2.59 -11.95 -22.32 1.75 18.54 -1.37 -3.59 -24.24
Cytoplasmic Foci -4.12 4.55 1.18 5.47 -3.46 -1.21 5.98 -6.38 -5.29 -13.12 -3.06 7.44 -5.53 -2.74 -13.59
Eisosomes -4.42 -6.18 3.61 5.25 7.68 3.38 3.76 6.15 6.56 9.00 -0.66 -0.12 6.67 7.03 10.22
Endoplasmic Reticulum 2.99 35.15* 28.17 31.23* -11.69 -16.83 10.07 20.15 43.59* 9.92 -11.11 27.91 32.02 52.45* 2.73
Endosome -3.12 11.73 4.16 6.72 -11.37 -5.87 3.82 -9.30 -4.03 -11.94 -6.55 9.43 -7.44 -1.44 -14.40
Golgi 0.13 3.50 -2.37 -2.28 -4.32 -3.42 -1.46 -4.92 0.22 -3.73 -2.83 0.61 -5.06 -1.51 -6.07
Lipid Particles -2.09 7.40 -16.17 -15.73 -24.54* -6.22 -9.82 -20.42 -13.77 -7.84 -6.10 -4.26 -25.99 -20.79 -20.69
Mitochondria -2.78 3.98 1.46 4.14 -4.14 -2.04 6.98 -2.56 1.19 -8.74 -2.73 8.21 -1.78 2.01 -9.68
Mitotic Spindle -1.05 -6.11 -1.98 -0.37 -1.03 5.71 0.65 -7.21 -7.42 -7.24 -0.27 -1.13 -7.40 -7.13 -7.27
None -0.49 1.83 -0.89 -0.56 -1.77 -2.41 1.32 -3.43 -1.58 -3.90 -1.93 2.42 -3.01 -1.57 -4.12
Nuclear Periphery 6.48 -16.89 -8.88 -17.83 12.14 5.64 -11.19 -5.77 -12.83 6.15 8.46 -19.58 -9.35 -20.31 13.22
Nuclear Periphery Foci 0.19 7.48 -4.15 -2.69 -12.39 -0.31 -3.37 -10.34 -10.15 -8.89 -0.05 2.30 -11.01 -9.97 -11.74
Nucleolus -2.04 -6.53 -4.76 -1.70 0.96 1.21 7.86 -8.39 -8.73 -11.58 0.24 2.38 -9.23 -8.93 -10.45
Nucleus -3.67 0.80 -1.62 2.28 -3.37 0.78 3.72 -6.66 -5.66 -8.02 -0.17 3.97 -6.84 -5.57 -8.56
Peroxisomes -0.57 9.05 6.28 6.97 -5.85 0.17 9.51 3.02 2.64 -9.47 0.19 12.36 4.96 4.74 -10.89
Vacuole 0.19 -45.16* -32.16 -33.71 11.41 7.09 -33.26 -26.35 -34.16 9.35 6.26 -52.57 -39.37 -47.03 15.26
Vacuole Periphery 0.41 -3.98 -1.66 -2.70 3.01 -1.96 -0.68 -6.98 -3.47 -6.80 -0.94 -4.77 -5.51 -4.40 -1.57
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Long chain fatty acyl-CoA synthetase; activates fatty acids with a preference for C12:0-C16:0 chain lengths; role in the competitive import of long-chain fatty acids and sphingoid long-chain bases; accounts for most acyl-CoA synthetase activity; localizes to lipid particles and the plasma membrane; role in sphingolipid-to-glycerolipid metabolism; forms ER foci upon replication stress; faa1 faa4 double null complemented by any of human ACSBG1, ACSL1, 3, 4, 5, 6, SLC27A2, or 4
Localization
Cell Percentages ER (66%)
Cell Cycle Regulation No
Subcompartmental Group ER-5

Faa1

Faa1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Faa1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available