Standard name | |
---|---|
Human Ortholog | |
Description | Long chain fatty acyl-CoA synthetase; activates fatty acids with a preference for C12:0-C16:0 chain lengths; role in the competitive import of long-chain fatty acids and sphingoid long-chain bases; accounts for most acyl-CoA synthetase activity; localizes to lipid particles and the plasma membrane; role in sphingolipid-to-glycerolipid metabolism; forms ER foci upon replication stress; faa1 faa4 double null complemented by any of human ACSBG1, ACSL1, 3, 4, 5, 6, SLC27A2, or 4 |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 20 | 4 | 25 | 18 | 28 | 11 | 24 | 14 | 16 | 19 | 11 | 10 | 27 | 33 | 37 | 10 | 2 | 7 | 5 | 2 | 4 | 0 | 9 | 4 |
Bud | 4 | 13 | 14 | 5 | 11 | 8 | 11 | 33 | 22 | 26 | 31 | 15 | 18 | 7 | 7 | 1 | 2 | 3 | 1 | 0 | 3 | 0 | 3 | 4 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 6 | 4 | 2 | 3 | 4 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 58 | 28 | 174 | 250 | 242 | 198 | 240 | 195 | 139 | 129 | 99 | 127 | 251 | 338 | 380 | 88 | 77 | 62 | 10 | 7 | 46 | 13 | 68 | 81 |
Cytoplasm | 61 | 88 | 100 | 154 | 124 | 222 | 281 | 309 | 256 | 269 | 242 | 236 | 85 | 111 | 118 | 44 | 39 | 31 | 18 | 60 | 72 | 28 | 38 | 39 |
Endoplasmic Reticulum | 100 | 46 | 129 | 164 | 119 | 146 | 184 | 197 | 64 | 86 | 79 | 110 | 62 | 100 | 77 | 1 | 4 | 4 | 184 | 112 | 277 | 142 | 304 | 353 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Golgi | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 4 | 2 | 0 | 0 | 0 | 0 | 1 | 2 |
Mitochondria | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 2 | 6 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 5 | 1 | 2 | 5 |
Nucleus | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 1 | 2 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 0 | 9 | 15 | 3 | 0 | 0 | 1 | 1 | 1 | 2 | 2 | 2 | 5 | 1 | 2 | 4 |
Unique Cell Count | 203 | 153 | 335 | 436 | 402 | 448 | 566 | 611 | 369 | 426 | 395 | 386 | 332 | 434 | 478 | 129 | 119 | 100 | 229 | 198 | 422 | 197 | 439 | 508 |
Labelled Cell Count | 243 | 180 | 443 | 592 | 526 | 585 | 740 | 754 | 499 | 544 | 487 | 501 | 444 | 589 | 622 | 150 | 129 | 113 | 229 | 198 | 422 | 197 | 439 | 508 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 21.9 | 20.2 | 25.1 | 21.7 | 22.4 | 21.0 | 20.0 | 21.4 | 19.7 | 18.5 | 17.2 | 20.5 | 23.5 | 26.6 | 26.8 | 30.3 | 30.4 | 29.8 | 22.5 | 23.3 | 24.2 |
Std Deviation (1e-4) | 5.2 | 4.6 | 5.4 | 3.6 | 4.4 | 4.4 | 4.2 | 4.3 | 4.2 | 4.4 | 4.2 | 5.1 | 5.3 | 6.8 | 7.6 | 9.3 | 9.4 | 7.7 | 4.7 | 5.9 | 5.5 |
Intensity Change (Log2) | – | – | – | -0.21 | -0.16 | -0.26 | -0.33 | -0.23 | -0.35 | -0.44 | -0.54 | -0.29 | -0.09 | 0.08 | 0.09 | 0.27 | 0.28 | 0.25 | -0.15 | -0.1 | -0.05 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -2.0 | -0.3 | -3.3 | -2.1 | -3.8 | -1.8 | -1.8 | -2.9 | -3.0 | 0.3 | 0.1 | 0.1 | 0.1 | -2.3 | -0.2 |
Bud | -2.7 | -1.1 | -2.0 | -2.0 | 0.8 | 1.1 | 1.2 | 2.1 | -0.2 | 0.8 | -2.2 | -2.4 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 1.5 | 2.3 | -2.1 | -2.8 | -6.0 | -3.8 | -6.1 | -7.5 | -5.2 | 6.4 | 7.6 | 8.3 | 3.2 | 2.4 | 1.8 |
Cytoplasm | 1.6 | 0.3 | 5.5 | 5.8 | 6.2 | 10.5 | 9.1 | 8.5 | 8.4 | -1.2 | -1.3 | -1.6 | 0.9 | 0.6 | 0.2 |
Endoplasmic Reticulum | -0.3 | -2.5 | -1.7 | -1.8 | -1.9 | -6.3 | -5.6 | -5.5 | -2.8 | -5.7 | -4.7 | -7.2 | -8.1 | -7.2 | -6.6 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 31.367 | 33.9957 | 32.4165 | 34.4882 | 30.9165 | 34.3222 | 45.6454 | 51.0226 | 47.5934 | 42.829 | 36.7373 | 45.9897 | 53.8104 | 57.5399 | 54.5025 | 48.0506 | 48.3153 | 62.7306 |
Actin | 0.0078 | 0.0004 | 0.003 | 0 | 0.0056 | 0.0007 | 0.0123 | 0.0104 | 0.0029 | 0.0002 | 0.0037 | 0.002 | 0.0057 | 0.0014 | 0.002 | 0.0224 | 0.0907 | 0.0005 |
Bud | 0.0022 | 0.0001 | 0.0006 | 0 | 0.0008 | 0.0001 | 0.0018 | 0.0001 | 0.0011 | 0.0002 | 0.0012 | 0.0003 | 0.0009 | 0.0001 | 0.0002 | 0.0009 | 0.0004 | 0.0002 |
Bud Neck | 0.0006 | 0.0005 | 0.0013 | 0.0006 | 0.0009 | 0.003 | 0.0008 | 0.0002 | 0.0005 | 0.0048 | 0.0028 | 0.0032 | 0.0014 | 0.0035 | 0.0031 | 0.0045 | 0.0026 | 0.0086 |
Bud Periphery | 0.0104 | 0.0007 | 0.0038 | 0.0007 | 0.0049 | 0.0013 | 0.0095 | 0.0005 | 0.0034 | 0.0042 | 0.0035 | 0.0026 | 0.0046 | 0.001 | 0.0019 | 0.0058 | 0.0013 | 0.0032 |
Bud Site | 0.0018 | 0.0021 | 0.0021 | 0 | 0.0012 | 0.0001 | 0.0029 | 0.001 | 0.003 | 0.0002 | 0.0248 | 0.0002 | 0.0024 | 0.0031 | 0.0007 | 0.0014 | 0.0008 | 0.0001 |
Cell Periphery | 0.1944 | 0.2384 | 0.2423 | 0.1764 | 0.2627 | 0.143 | 0.3266 | 0.3099 | 0.3477 | 0.3838 | 0.2646 | 0.1916 | 0.2398 | 0.3112 | 0.2902 | 0.3101 | 0.1116 | 0.2048 |
Cytoplasm | 0.0441 | 0.0844 | 0.0336 | 0.0077 | 0.0348 | 0.0263 | 0.0159 | 0.0164 | 0.0101 | 0.0054 | 0.0079 | 0.0143 | 0.0693 | 0.0641 | 0.0326 | 0.0302 | 0.0116 | 0.0135 |
Cytoplasmic Foci | 0.007 | 0.0005 | 0.0063 | 0 | 0.0122 | 0.0003 | 0.0019 | 0.0001 | 0.004 | 0 | 0.0087 | 0.0029 | 0.0057 | 0.001 | 0.0047 | 0.0152 | 0.0067 | 0.0001 |
Eisosomes | 0.0009 | 0.0015 | 0.0008 | 0.0001 | 0.0006 | 0.0002 | 0.0016 | 0.0008 | 0.0005 | 0.0017 | 0.0003 | 0.0003 | 0.0008 | 0.0005 | 0.0019 | 0.0005 | 0.0005 | 0.0007 |
Endoplasmic Reticulum | 0.6348 | 0.5759 | 0.6158 | 0.7785 | 0.4681 | 0.7911 | 0.5305 | 0.5888 | 0.5438 | 0.5204 | 0.5322 | 0.7452 | 0.5104 | 0.5058 | 0.5406 | 0.4139 | 0.6643 | 0.7043 |
Endosome | 0.0071 | 0.0006 | 0.0019 | 0.0001 | 0.0403 | 0.0005 | 0.0057 | 0.0001 | 0.0014 | 0 | 0.0045 | 0.0008 | 0.0119 | 0.0002 | 0.0147 | 0.0139 | 0.0388 | 0.0001 |
Golgi | 0.0021 | 0.0007 | 0.0022 | 0.0001 | 0.043 | 0.0004 | 0.0033 | 0.0004 | 0.0013 | 0.0002 | 0.0047 | 0.0016 | 0.0043 | 0.0006 | 0.002 | 0.0134 | 0.0133 | 0.0006 |
Lipid Particles | 0.0119 | 0.0574 | 0.0254 | 0.009 | 0.0421 | 0.0142 | 0.0068 | 0.0119 | 0.0066 | 0.0035 | 0.0487 | 0.0055 | 0.0154 | 0.0449 | 0.016 | 0.0327 | 0.0122 | 0.0123 |
Mitochondria | 0.0088 | 0.0037 | 0.0079 | 0 | 0.038 | 0.0002 | 0.0043 | 0.0002 | 0.0031 | 0 | 0.0553 | 0.0027 | 0.01 | 0 | 0.0003 | 0.0167 | 0.0031 | 0 |
None | 0.003 | 0.0003 | 0.001 | 0 | 0.0009 | 0.0002 | 0.0006 | 0 | 0.0006 | 0 | 0.0001 | 0.0001 | 0.0011 | 0.0001 | 0.0002 | 0.0022 | 0.0022 | 0 |
Nuclear Periphery | 0.0228 | 0.012 | 0.0153 | 0.0105 | 0.0125 | 0.0075 | 0.0399 | 0.0329 | 0.0391 | 0.0449 | 0.0167 | 0.0119 | 0.0676 | 0.0338 | 0.0458 | 0.0437 | 0.0225 | 0.0296 |
Nucleolus | 0.001 | 0.0002 | 0.0006 | 0 | 0.0004 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0003 | 0.0002 | 0.0005 | 0.0001 | 0.0001 | 0.0023 | 0.0002 | 0.0001 |
Nucleus | 0.0026 | 0.0004 | 0.0015 | 0.0002 | 0.001 | 0.0004 | 0.001 | 0.0002 | 0.0008 | 0.0004 | 0.0003 | 0.0004 | 0.0043 | 0.0006 | 0.0007 | 0.0014 | 0.0012 | 0.0007 |
Peroxisomes | 0.0005 | 0 | 0.0054 | 0 | 0.0048 | 0 | 0.0007 | 0 | 0.0006 | 0 | 0.006 | 0.0007 | 0.0016 | 0 | 0.0053 | 0.0122 | 0.006 | 0 |
Punctate Nuclear | 0.0026 | 0.0001 | 0.0006 | 0 | 0.0005 | 0 | 0.0005 | 0 | 0.0001 | 0 | 0.0003 | 0 | 0.0004 | 0.0001 | 0.0003 | 0.002 | 0.0036 | 0 |
Vacuole | 0.0315 | 0.0193 | 0.0272 | 0.0157 | 0.0182 | 0.01 | 0.0317 | 0.0258 | 0.0281 | 0.0291 | 0.0131 | 0.0129 | 0.0389 | 0.0275 | 0.0357 | 0.0499 | 0.0048 | 0.0202 |
Vacuole Periphery | 0.0018 | 0.0009 | 0.0013 | 0.0002 | 0.0066 | 0.0002 | 0.0015 | 0.0002 | 0.001 | 0.0006 | 0.0004 | 0.0006 | 0.0032 | 0.0002 | 0.0009 | 0.0046 | 0.0015 | 0.0004 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 54.3045 | 30.9562 | 32.716 | 60.3837 | 68.0272 | 62.4025 | 43.2743 | 43.2107 | 53.131 | 68.7882 |
Translational Efficiency | 1.8731 | 2.4067 | 1.7957 | 1.5223 | 1.6543 | 1.6868 | 1.3888 | 1.3295 | 1.6198 | 1.316 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
593 | 1201 | 889 | 1149 | 1123 | 1581 | 1197 | 2251 | 1716 | 2782 | 2086 | 3400 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1589.66 | 1668.14 | 4376.84 | 4181.24 | 1585.86 | 1711.49 | 3684.63 | 4115.46 | 1587.17 | 1692.78 | 3979.63 | 4137.69 |
Standard Deviation | 300.65 | 419.22 | 918.20 | 849.66 | 379.68 | 403.17 | 738.88 | 1052.30 | 354.37 | 410.74 | 888.69 | 988.97 |
Intensity Change Log 2 | — | 0.069522 | 1.461171 | 1.395213 | — | 0.109987 | 1.216254 | 1.375788 | — | 0.089872 | 1.344050 | 1.385545 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000610 | 0.003989 | 0.000692 | 0.000586 | 0.001606 | 0.001223 | 0.000681 | 0.000944 | 0.001262 | 0.002417 | 0.000686 | 0.000823 |
Bud Neck | 0.003370 | 0.005226 | 0.001886 | 0.005106 | 0.005967 | 0.002901 | 0.002381 | 0.006984 | 0.005070 | 0.003905 | 0.002170 | 0.006350 |
Bud Site | 0.010354 | 0.046236 | 0.002827 | 0.011727 | 0.022930 | 0.014893 | 0.005874 | 0.025223 | 0.018584 | 0.028424 | 0.004576 | 0.020662 |
Cell Periphery | 0.293242* | 0.223705* | 0.574382* | 0.359562* | 0.353032* | 0.187643* | 0.345853* | 0.256182* | 0.332370* | 0.203211* | 0.443246* | 0.291119* |
Cytoplasm | 0.044310 | 0.102624* | 0.001531 | 0.026927 | 0.113927* | 0.064662 | 0.020221 | 0.123665* | 0.089869 | 0.081050 | 0.012256 | 0.090973 |
Cytoplasmic Foci | 0.003128 | 0.007203 | 0.000103 | 0.001804 | 0.005214 | 0.006531 | 0.000542 | 0.010858 | 0.004493 | 0.006821 | 0.000355 | 0.007798 |
Eisosomes | 0.000353 | 0.001156 | 0.001055 | 0.000217 | 0.000864 | 0.000460 | 0.000430 | 0.000182 | 0.000687 | 0.000760 | 0.000696 | 0.000194 |
Endoplasmic Reticulum | 0.506700* | 0.457750* | 0.028046 | 0.102424* | 0.338313* | 0.550283* | 0.218258* | 0.136425* | 0.396503* | 0.510336* | 0.137195* | 0.124935* |
Endosome | 0.005231 | 0.007215 | 0.000681 | 0.003278 | 0.004676 | 0.007950 | 0.002987 | 0.010107 | 0.004868 | 0.007633 | 0.002004 | 0.007799 |
Golgi | 0.006111 | 0.006007 | 0.004287 | 0.008762 | 0.004804 | 0.007524 | 0.005577 | 0.007319 | 0.005255 | 0.006869 | 0.005027 | 0.007807 |
Lipid Particles | 0.054485 | 0.068519 | 0.013302 | 0.205070* | 0.052135 | 0.084696 | 0.114399* | 0.175764* | 0.052947 | 0.077712 | 0.071314 | 0.185668* |
Mitochondria | 0.000545 | 0.001008 | 0.000165 | 0.000380 | 0.000825 | 0.001567 | 0.000242 | 0.001159 | 0.000728 | 0.001326 | 0.000209 | 0.000896 |
Mitotic Spindle | 0.000804 | 0.001396 | 0.001240 | 0.001581 | 0.000980 | 0.000651 | 0.000874 | 0.008884 | 0.000919 | 0.000973 | 0.001030 | 0.006416 |
None | 0.000589 | 0.000844 | 0.000033 | 0.001287 | 0.000413 | 0.001033 | 0.000211 | 0.001702 | 0.000473 | 0.000951 | 0.000135 | 0.001562 |
Nuclear Periphery | 0.005377 | 0.002563 | 0.023614 | 0.010544 | 0.006394 | 0.003913 | 0.013313 | 0.009978 | 0.006043 | 0.003330 | 0.017703 | 0.010170 |
Nuclear Periphery Foci | 0.004698 | 0.004465 | 0.001871 | 0.006494 | 0.004297 | 0.004358 | 0.005210 | 0.011555 | 0.004435 | 0.004404 | 0.003787 | 0.009845 |
Nucleolus | 0.000044 | 0.000062 | 0.000086 | 0.000077 | 0.000086 | 0.000076 | 0.000042 | 0.000204 | 0.000072 | 0.000070 | 0.000061 | 0.000162 |
Nucleus | 0.000689 | 0.001503 | 0.000525 | 0.000972 | 0.001698 | 0.001303 | 0.000889 | 0.003702 | 0.001349 | 0.001390 | 0.000734 | 0.002780 |
Peroxisomes | 0.004817 | 0.005237 | 0.000201 | 0.001393 | 0.007302 | 0.007146 | 0.000963 | 0.005272 | 0.006443 | 0.006322 | 0.000639 | 0.003961 |
Vacuole | 0.050976 | 0.050169 | 0.335823* | 0.246459* | 0.072287 | 0.048008 | 0.258649* | 0.198517 | 0.064923 | 0.048941 | 0.291538* | 0.214718* |
Vacuole Periphery | 0.003566 | 0.003124 | 0.007651 | 0.005347 | 0.002250 | 0.003179 | 0.002403 | 0.005371 | 0.002705 | 0.003155 | 0.004639 | 0.005363 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -7.45 | -0.39 | -0.19 | 7.27 | 0.25 | 1.06 | 2.52 | 1.94 | 0.91 | -0.94 | -4.05 | 2.06 | 1.74 | 6.21 | -0.63 |
Bud Neck | -2.31 | 1.61 | -1.99 | -0.48 | -3.16 | 3.07 | 3.60 | -1.69 | -6.24 | -7.27 | 1.63 | 3.97 | -2.48 | -4.94 | -7.58 |
Bud Site | -8.27 | 3.15 | -1.03 | 7.59 | -4.53 | 2.88 | 6.35 | -1.64 | -4.92 | -8.80 | -3.97 | 7.34 | -1.97 | 2.28 | -10.24 |
Cell Periphery | 5.67 | -23.57* | -5.16 | -13.93 | 24.10* | 17.46 | 0.94 | 11.94 | -7.53 | 13.38 | 17.08 | -13.74 | 6.88 | -13.24 | 25.32* |
Cytoplasm | -8.60 | 9.47 | 3.29 | 13.25 | -9.99 | 7.66 | 15.94 | -2.59 | -11.95 | -22.32 | 1.75 | 18.54 | -1.37 | -3.59 | -24.24 |
Cytoplasmic Foci | -4.12 | 4.55 | 1.18 | 5.47 | -3.46 | -1.21 | 5.98 | -6.38 | -5.29 | -13.12 | -3.06 | 7.44 | -5.53 | -2.74 | -13.59 |
Eisosomes | -4.42 | -6.18 | 3.61 | 5.25 | 7.68 | 3.38 | 3.76 | 6.15 | 6.56 | 9.00 | -0.66 | -0.12 | 6.67 | 7.03 | 10.22 |
Endoplasmic Reticulum | 2.99 | 35.15* | 28.17 | 31.23* | -11.69 | -16.83 | 10.07 | 20.15 | 43.59* | 9.92 | -11.11 | 27.91 | 32.02 | 52.45* | 2.73 |
Endosome | -3.12 | 11.73 | 4.16 | 6.72 | -11.37 | -5.87 | 3.82 | -9.30 | -4.03 | -11.94 | -6.55 | 9.43 | -7.44 | -1.44 | -14.40 |
Golgi | 0.13 | 3.50 | -2.37 | -2.28 | -4.32 | -3.42 | -1.46 | -4.92 | 0.22 | -3.73 | -2.83 | 0.61 | -5.06 | -1.51 | -6.07 |
Lipid Particles | -2.09 | 7.40 | -16.17 | -15.73 | -24.54* | -6.22 | -9.82 | -20.42 | -13.77 | -7.84 | -6.10 | -4.26 | -25.99 | -20.79 | -20.69 |
Mitochondria | -2.78 | 3.98 | 1.46 | 4.14 | -4.14 | -2.04 | 6.98 | -2.56 | 1.19 | -8.74 | -2.73 | 8.21 | -1.78 | 2.01 | -9.68 |
Mitotic Spindle | -1.05 | -6.11 | -1.98 | -0.37 | -1.03 | 5.71 | 0.65 | -7.21 | -7.42 | -7.24 | -0.27 | -1.13 | -7.40 | -7.13 | -7.27 |
None | -0.49 | 1.83 | -0.89 | -0.56 | -1.77 | -2.41 | 1.32 | -3.43 | -1.58 | -3.90 | -1.93 | 2.42 | -3.01 | -1.57 | -4.12 |
Nuclear Periphery | 6.48 | -16.89 | -8.88 | -17.83 | 12.14 | 5.64 | -11.19 | -5.77 | -12.83 | 6.15 | 8.46 | -19.58 | -9.35 | -20.31 | 13.22 |
Nuclear Periphery Foci | 0.19 | 7.48 | -4.15 | -2.69 | -12.39 | -0.31 | -3.37 | -10.34 | -10.15 | -8.89 | -0.05 | 2.30 | -11.01 | -9.97 | -11.74 |
Nucleolus | -2.04 | -6.53 | -4.76 | -1.70 | 0.96 | 1.21 | 7.86 | -8.39 | -8.73 | -11.58 | 0.24 | 2.38 | -9.23 | -8.93 | -10.45 |
Nucleus | -3.67 | 0.80 | -1.62 | 2.28 | -3.37 | 0.78 | 3.72 | -6.66 | -5.66 | -8.02 | -0.17 | 3.97 | -6.84 | -5.57 | -8.56 |
Peroxisomes | -0.57 | 9.05 | 6.28 | 6.97 | -5.85 | 0.17 | 9.51 | 3.02 | 2.64 | -9.47 | 0.19 | 12.36 | 4.96 | 4.74 | -10.89 |
Vacuole | 0.19 | -45.16* | -32.16 | -33.71 | 11.41 | 7.09 | -33.26 | -26.35 | -34.16 | 9.35 | 6.26 | -52.57 | -39.37 | -47.03 | 15.26 |
Vacuole Periphery | 0.41 | -3.98 | -1.66 | -2.70 | 3.01 | -1.96 | -0.68 | -6.98 | -3.47 | -6.80 | -0.94 | -4.77 | -5.51 | -4.40 | -1.57 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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