Standard name
Human Ortholog
Description Long chain fatty acyl-CoA synthetase; activates fatty acids with a preference for C12:0-C16:0 chain lengths; role in the competitive import of long-chain fatty acids and sphingoid long-chain bases; accounts for most acyl-CoA synthetase activity; localizes to lipid particles and the plasma membrane; role in sphingolipid-to-glycerolipid metabolism; forms ER foci upon replication stress; faa1 faa4 double null complemented by any of human ACSBG1, ACSL1, 3, 4, 5, 6, SLC27A2, or 4
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.1 0 0.07 0 0.07 0 0 0 0 0 0 0 0.08 0.08 0.08 0.08 0 0.07 0 0 0 0 0 0
Bud 0 0.08 0 0 0 0 0 0.05 0.06 0.06 0.08 0 0.05 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.29 0.18 0.52 0.57 0.6 0.44 0.42 0.32 0.38 0.3 0.25 0.33 0.76 0.78 0.79 0.68 0.65 0.62 0.05 0 0.11 0.07 0.16 0.16
Cytoplasm 0.3 0.58 0.3 0.35 0.31 0.5 0.5 0.51 0.69 0.63 0.61 0.61 0.26 0.26 0.25 0.34 0.33 0.31 0.08 0.31 0.17 0.15 0.09 0.08
Endoplasmic Reticulum 0.49 0.3 0.39 0.38 0.3 0.33 0.33 0.32 0.17 0.2 0.2 0.28 0.19 0.23 0.16 0 0 0 0.81 0.57 0.66 0.72 0.69 0.7
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 20 4 25 18 28 11 24 14 16 19 11 10 27 33 37 10 2 7 5 2 4 0 9 4
Bud 4 13 14 5 11 8 11 33 22 26 31 15 18 7 7 1 2 3 1 0 3 0 3 4
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 6 4 2 3 4
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 58 28 174 250 242 198 240 195 139 129 99 127 251 338 380 88 77 62 10 7 46 13 68 81
Cytoplasm 61 88 100 154 124 222 281 309 256 269 242 236 85 111 118 44 39 31 18 60 72 28 38 39
Endoplasmic Reticulum 100 46 129 164 119 146 184 197 64 86 79 110 62 100 77 1 4 4 184 112 277 142 304 353
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Golgi 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 2 4 2 0 0 0 0 1 2
Mitochondria 0 1 0 0 1 0 0 0 2 2 6 0 0 0 0 1 0 0 0 1 5 1 2 5
Nucleus 0 0 0 0 1 0 0 2 0 1 2 0 1 0 1 1 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 3 2 0 0 0 0 0 0 0 1 0 0 1 0 1
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 1 0 2 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 4 0 9 15 3 0 0 1 1 1 2 2 2 5 1 2 4
Unique Cell Count 203 153 335 436 402 448 566 611 369 426 395 386 332 434 478 129 119 100 229 198 422 197 439 508
Labelled Cell Count 243 180 443 592 526 585 740 754 499 544 487 501 444 589 622 150 129 113 229 198 422 197 439 508


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 21.9 20.2 25.1 21.7 22.4 21.0 20.0 21.4 19.7 18.5 17.2 20.5 23.5 26.6 26.8 30.3 30.4 29.8 22.5 23.3 24.2
Std Deviation (1e-4) 5.2 4.6 5.4 3.6 4.4 4.4 4.2 4.3 4.2 4.4 4.2 5.1 5.3 6.8 7.6 9.3 9.4 7.7 4.7 5.9 5.5
Intensity Change (Log2) – – – -0.21 -0.16 -0.26 -0.33 -0.23 -0.35 -0.44 -0.54 -0.29 -0.09 0.08 0.09 0.27 0.28 0.25 -0.15 -0.1 -0.05


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -2.0 -0.3 -3.3 -2.1 -3.8 -1.8 -1.8 -2.9 -3.0 0.3 0.1 0.1 0.1 -2.3 -0.2
Bud -2.7 -1.1 -2.0 -2.0 0.8 1.1 1.2 2.1 -0.2 0.8 -2.2 -2.4 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1.5 2.3 -2.1 -2.8 -6.0 -3.8 -6.1 -7.5 -5.2 6.4 7.6 8.3 3.2 2.4 1.8
Cytoplasm 1.6 0.3 5.5 5.8 6.2 10.5 9.1 8.5 8.4 -1.2 -1.3 -1.6 0.9 0.6 0.2
Endoplasmic Reticulum -0.3 -2.5 -1.7 -1.8 -1.9 -6.3 -5.6 -5.5 -2.8 -5.7 -4.7 -7.2 -8.1 -7.2 -6.6
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 3.6 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 31.367 33.9957 32.4165 34.4882 30.9165 34.3222 45.6454 51.0226 47.5934 42.829 36.7373 45.9897 53.8104 57.5399 54.5025 48.0506 48.3153 62.7306
Actin 0.0078 0.0004 0.003 0 0.0056 0.0007 0.0123 0.0104 0.0029 0.0002 0.0037 0.002 0.0057 0.0014 0.002 0.0224 0.0907 0.0005
Bud 0.0022 0.0001 0.0006 0 0.0008 0.0001 0.0018 0.0001 0.0011 0.0002 0.0012 0.0003 0.0009 0.0001 0.0002 0.0009 0.0004 0.0002
Bud Neck 0.0006 0.0005 0.0013 0.0006 0.0009 0.003 0.0008 0.0002 0.0005 0.0048 0.0028 0.0032 0.0014 0.0035 0.0031 0.0045 0.0026 0.0086
Bud Periphery 0.0104 0.0007 0.0038 0.0007 0.0049 0.0013 0.0095 0.0005 0.0034 0.0042 0.0035 0.0026 0.0046 0.001 0.0019 0.0058 0.0013 0.0032
Bud Site 0.0018 0.0021 0.0021 0 0.0012 0.0001 0.0029 0.001 0.003 0.0002 0.0248 0.0002 0.0024 0.0031 0.0007 0.0014 0.0008 0.0001
Cell Periphery 0.1944 0.2384 0.2423 0.1764 0.2627 0.143 0.3266 0.3099 0.3477 0.3838 0.2646 0.1916 0.2398 0.3112 0.2902 0.3101 0.1116 0.2048
Cytoplasm 0.0441 0.0844 0.0336 0.0077 0.0348 0.0263 0.0159 0.0164 0.0101 0.0054 0.0079 0.0143 0.0693 0.0641 0.0326 0.0302 0.0116 0.0135
Cytoplasmic Foci 0.007 0.0005 0.0063 0 0.0122 0.0003 0.0019 0.0001 0.004 0 0.0087 0.0029 0.0057 0.001 0.0047 0.0152 0.0067 0.0001
Eisosomes 0.0009 0.0015 0.0008 0.0001 0.0006 0.0002 0.0016 0.0008 0.0005 0.0017 0.0003 0.0003 0.0008 0.0005 0.0019 0.0005 0.0005 0.0007
Endoplasmic Reticulum 0.6348 0.5759 0.6158 0.7785 0.4681 0.7911 0.5305 0.5888 0.5438 0.5204 0.5322 0.7452 0.5104 0.5058 0.5406 0.4139 0.6643 0.7043
Endosome 0.0071 0.0006 0.0019 0.0001 0.0403 0.0005 0.0057 0.0001 0.0014 0 0.0045 0.0008 0.0119 0.0002 0.0147 0.0139 0.0388 0.0001
Golgi 0.0021 0.0007 0.0022 0.0001 0.043 0.0004 0.0033 0.0004 0.0013 0.0002 0.0047 0.0016 0.0043 0.0006 0.002 0.0134 0.0133 0.0006
Lipid Particles 0.0119 0.0574 0.0254 0.009 0.0421 0.0142 0.0068 0.0119 0.0066 0.0035 0.0487 0.0055 0.0154 0.0449 0.016 0.0327 0.0122 0.0123
Mitochondria 0.0088 0.0037 0.0079 0 0.038 0.0002 0.0043 0.0002 0.0031 0 0.0553 0.0027 0.01 0 0.0003 0.0167 0.0031 0
None 0.003 0.0003 0.001 0 0.0009 0.0002 0.0006 0 0.0006 0 0.0001 0.0001 0.0011 0.0001 0.0002 0.0022 0.0022 0
Nuclear Periphery 0.0228 0.012 0.0153 0.0105 0.0125 0.0075 0.0399 0.0329 0.0391 0.0449 0.0167 0.0119 0.0676 0.0338 0.0458 0.0437 0.0225 0.0296
Nucleolus 0.001 0.0002 0.0006 0 0.0004 0.0001 0.0002 0.0001 0.0002 0.0001 0.0003 0.0002 0.0005 0.0001 0.0001 0.0023 0.0002 0.0001
Nucleus 0.0026 0.0004 0.0015 0.0002 0.001 0.0004 0.001 0.0002 0.0008 0.0004 0.0003 0.0004 0.0043 0.0006 0.0007 0.0014 0.0012 0.0007
Peroxisomes 0.0005 0 0.0054 0 0.0048 0 0.0007 0 0.0006 0 0.006 0.0007 0.0016 0 0.0053 0.0122 0.006 0
Punctate Nuclear 0.0026 0.0001 0.0006 0 0.0005 0 0.0005 0 0.0001 0 0.0003 0 0.0004 0.0001 0.0003 0.002 0.0036 0
Vacuole 0.0315 0.0193 0.0272 0.0157 0.0182 0.01 0.0317 0.0258 0.0281 0.0291 0.0131 0.0129 0.0389 0.0275 0.0357 0.0499 0.0048 0.0202
Vacuole Periphery 0.0018 0.0009 0.0013 0.0002 0.0066 0.0002 0.0015 0.0002 0.001 0.0006 0.0004 0.0006 0.0032 0.0002 0.0009 0.0046 0.0015 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 54.3045 30.9562 32.716 60.3837 68.0272 62.4025 43.2743 43.2107 53.131 68.7882
Translational Efficiency 1.8731 2.4067 1.7957 1.5223 1.6543 1.6868 1.3888 1.3295 1.6198 1.316

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Long chain fatty acyl-CoA synthetase; activates fatty acids with a preference for C12:0-C16:0 chain lengths; role in the competitive import of long-chain fatty acids and sphingoid long-chain bases; accounts for most acyl-CoA synthetase activity; localizes to lipid particles and the plasma membrane; role in sphingolipid-to-glycerolipid metabolism; forms ER foci upon replication stress; faa1 faa4 double null complemented by any of human ACSBG1, ACSL1, 3, 4, 5, 6, SLC27A2, or 4
Localization
Cell Percentages ER (66%)
Cell Cycle Regulation No
Subcompartmental Group ER-5

Faa1

Faa1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Faa1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available