Standard name
Human Ortholog
Description N-acetylglucosaminyltransferase; capable of modification of N-linked glycans in the Golgi apparatus
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 0.76 0.94 0.9 0.97 0.87 0.83 0.87 0.9 – 0.92 0.9 0.88 0.99 0.99 1.0 – 0.79 0.66 0.21 0.37 0.21 0.36 0.35 0.39
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0.08 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0.05 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0.13 0 0.05 0 0 0.16 0.22 0.15 – 0.21 0.26 0.29 0 0 0 – 0 0 0 0 0.07 0 0 0.05
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0.05 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.18 0.05 0.11 0 0.21 0.18 0.12 0.08 – 0 0 0 0 0 0 – 0.29 0.36 0.65 0.55 0.62 0.49 0.52 0.46
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 1 1 0 – 0 0 0 0 1 1 0 2
Bud 0 3 1 2 0 2 2 6 – 3 14 7 0 0 0 – 0 1 1 2 2 4 0 3
Bud Neck 1 1 0 1 0 0 0 0 – 0 1 0 1 1 1 – 0 0 0 3 1 0 2 1
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 3 0 – 0 0 0 2 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 118 483 249 206 210 245 356 522 – 384 367 382 137 202 261 – 324 275 46 200 58 71 80 103
Endoplasmic Reticulum 1 3 1 1 1 1 0 0 – 0 0 0 0 0 0 – 32 20 1 0 7 6 3 4
Endosome 1 0 0 0 1 0 0 5 – 1 1 0 0 0 0 – 7 13 10 9 3 3 4 2
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 1 0 0 0 0 0 0 1
Mitochondria 20 22 13 7 8 47 89 85 – 87 105 128 0 0 0 – 17 11 8 9 18 8 8 14
Nucleus 2 0 0 0 1 2 2 4 – 1 1 0 0 3 2 – 10 22 0 1 0 0 0 0
Nuclear Periphery 1 0 1 1 0 5 4 1 – 2 2 2 0 0 0 – 0 0 0 0 0 0 0 1
Nucleolus 0 0 0 0 0 1 0 0 – 0 1 0 0 0 0 – 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 2 – 0 1 1 0 0 0 – 0 3 4 2 2 0 1 1
Vac/Vac Membrane 28 25 30 8 52 54 48 45 – 13 16 18 0 0 0 – 118 151 144 296 172 97 118 121
Unique Cell Count 155 515 276 212 242 295 409 582 417 406 436 138 205 262 411 416 224 535 277 200 226 265
Labelled Cell Count 172 537 295 226 273 357 504 670 491 509 538 141 207 264 509 497 224 535 277 200 226 265


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.0 3.8 3.6 3.8 3.8 3.4 3.1 3.6 – 2.8 3.1 3.0 5.2 5.2 5.2 4.4 5.2 5.6 6.1 5.5 5.8
Std Deviation (1e-4) 0.7 0.6 0.9 1.2 0.8 1.1 0.8 1.2 – 0.4 1.2 0.8 0.9 1.1 1.1 1.3 1.6 1.6 10.5 1.8 2.1
Intensity Change (Log2) – – – 0.08 0.09 -0.07 -0.22 -0.02 – -0.37 -0.2 -0.28 0.51 0.53 0.54 0.3 0.52 0.63 0.76 0.6 0.69


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – 0 0
Bud Neck – – – – – – – – – – – – – 0 0
Bud Site – – – – – – – – – – – – – 0 0
Cell Periphery – – – – – – – – – – – – – 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – 0 0
Endosome – – – – – – – – – – – – – 0 0
Golgi – – – – – – – – – – – – – 0 0
Mitochondria – – – – – – – – – – – – – 0 0
Nuclear Periphery – – – – – – – – – – – – – 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – 0 0
Peroxisomes – – – – – – – – – – – – – 0 0
SpindlePole – – – – – – – – – – – – – 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – 0 0
Cytoplasm – – – – – – – – – – – – – 0 0
Nucleus – – – – – – – – – – – – – 0 0
Vacuole – – – – – – – – – – – – – 5.6 7.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.0434 0.8747 0.9191 0.6737 0.9617 1.1745 2.136 2.3917 2.9179 2.2818 2.788 2.6313 8.5412 10.1076 8.5743 9.5246 13.7033 11.9873
Actin 0.0383 0.002 0.0033 0.0005 0.0152 0.0081 0.02 0.0001 0.0091 0.0026 0.0248 0.0027 0.001 0.001 0.0025 0.0019 0.009 0.0026
Bud 0.0014 0.001 0.0078 0.0004 0.0029 0.0021 0.0035 0.0042 0.0018 0.0295 0.0061 0.0014 0.0243 0.0417 0.0347 0.0355 0.0042 0.0208
Bud Neck 0.0043 0.0023 0.0033 0.0004 0.0043 0.0022 0.0016 0.0011 0.0058 0.0164 0.0024 0.0056 0.0007 0.0006 0.0008 0.0012 0.0015 0.0033
Bud Periphery 0.0039 0.0021 0.0042 0.0005 0.0079 0.0048 0.0131 0.004 0.0033 0.0113 0.0204 0.0015 0.1072 0.0767 0.104 0.1074 0.0424 0.104
Bud Site 0.0072 0.008 0.0087 0.0003 0.0182 0.0013 0.0049 0.0091 0.0076 0.0248 0.0047 0.002 0.0199 0.0426 0.0194 0.0107 0.0009 0.0032
Cell Periphery 0.0016 0.0008 0.001 0.0002 0.0013 0.0007 0.0069 0.0012 0.0077 0.0016 0.002 0.0006 0.2232 0.2019 0.182 0.1723 0.2634 0.2476
Cytoplasm 0.1047 0.232 0.1287 0.1229 0.1193 0.1931 0.1418 0.1828 0.1565 0.1127 0.0831 0.1918 0.0968 0.1519 0.1049 0.0931 0.1024 0.1526
Cytoplasmic Foci 0.0591 0.0345 0.0958 0.0832 0.095 0.0426 0.067 0.044 0.0628 0.0709 0.1187 0.0625 0.0333 0.0198 0.0345 0.0619 0.046 0.0274
Eisosomes 0.0005 0.0002 0.004 0.0001 0.0006 0.0001 0.0011 0.0001 0.0002 0.0002 0.0003 0.0001 0.0007 0.0007 0.0003 0.0003 0.0018 0.0004
Endoplasmic Reticulum 0.0083 0.0042 0.0024 0.0027 0.0046 0.0035 0.0093 0.0036 0.0037 0.0051 0.0059 0.003 0.0501 0.0211 0.0217 0.0677 0.1031 0.0537
Endosome 0.0954 0.0911 0.1155 0.2822 0.145 0.1024 0.1145 0.1194 0.1229 0.2118 0.2351 0.1378 0.0447 0.0595 0.0759 0.0664 0.0204 0.0523
Golgi 0.0072 0.0017 0.0042 0.0124 0.0075 0.0055 0.0076 0.0017 0.0066 0.0048 0.0171 0.0046 0.0007 0.001 0.0013 0.0014 0.007 0.0007
Lipid Particles 0.0171 0.0038 0.0217 0.003 0.0178 0.0082 0.014 0.004 0.0066 0.0085 0.0227 0.0043 0.0056 0.0079 0.0032 0.0058 0.0033 0.0036
Mitochondria 0.0232 0.0023 0.0131 0.0072 0.0233 0.0111 0.0149 0.0036 0.0165 0.0162 0.0225 0.0061 0.0037 0.0062 0.0036 0.0125 0.0013 0.0022
None 0.4939 0.4742 0.4769 0.3764 0.3864 0.4478 0.4524 0.3882 0.4458 0.2478 0.1913 0.3736 0.2883 0.2468 0.3007 0.2422 0.3601 0.2223
Nuclear Periphery 0.0063 0.0032 0.0023 0.0022 0.0083 0.0086 0.0055 0.0037 0.0035 0.0051 0.0094 0.0028 0.0086 0.0069 0.0042 0.0053 0.0051 0.0058
Nucleolus 0.0044 0.0039 0.0062 0.0009 0.0134 0.0031 0.0022 0.0031 0.0025 0.0044 0.0023 0.0019 0.002 0.0019 0.0026 0.0039 0.0008 0.0012
Nucleus 0.0069 0.0187 0.0067 0.0027 0.0118 0.0116 0.0046 0.0093 0.0064 0.0116 0.0063 0.0096 0.0033 0.0088 0.0058 0.0048 0.0013 0.0053
Peroxisomes 0.0169 0.0006 0.0145 0.0015 0.0209 0.0024 0.0117 0.0007 0.0055 0.0058 0.0149 0.0073 0.0006 0.0007 0.0011 0.0117 0.0003 0.0005
Punctate Nuclear 0.0178 0.0021 0.0035 0.0011 0.0073 0.0024 0.0045 0.0027 0.0034 0.0047 0.0317 0.0023 0.0013 0.0037 0.0041 0.0097 0.0015 0.0025
Vacuole 0.0741 0.1057 0.0688 0.0875 0.0822 0.1279 0.0909 0.1995 0.1098 0.1844 0.1519 0.1661 0.0793 0.092 0.0842 0.0782 0.023 0.0811
Vacuole Periphery 0.0074 0.0057 0.0075 0.0118 0.0067 0.0105 0.0079 0.0138 0.0121 0.02 0.0264 0.0126 0.0048 0.0064 0.0086 0.0062 0.0012 0.007

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 42.6111 40.0966 29.7534 44.3269 42.1623 42.5876 44.9336 42.1844 42.6115 61.8023
Translational Efficiency 0.8916 0.5645 0.7438 0.4721 0.5393 0.785 0.6083 0.5578 0.588 0.4455

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
93 485 2288 1450 2424 1385 3003 1270 2517 1870 5291 2720

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 862.12 768.10 797.76 793.78 698.44 698.71 823.05 765.54 704.49 716.71 812.11 780.59
Standard Deviation 124.79 97.37 86.70 106.41 78.90 94.66 92.12 100.91 86.74 100.10 90.69 104.83
Intensity Change Log 2 — -0.166595 -0.111934 -0.119149 — 0.000558 0.236844 0.132342 — -0.089382 0.054651 -0.001147

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001253 0.000202 0.000108 0.000221 0.000101 0.000195 0.000098 0.000233 0.000143 0.000197 0.000102 0.000226
Bud Neck 0.002901 0.026920 0.005631 0.005132 0.029882 0.033930 0.006618 0.010646 0.028885 0.032112 0.006191 0.007706
Bud Site 0.065682 0.012095 0.007827 0.016014 0.007754 0.010523 0.007388 0.022194 0.009895 0.010931 0.007578 0.018899
Cell Periphery 0.000471 0.000099 0.000071 0.000219 0.000110 0.000106 0.000051 0.000189 0.000124 0.000105 0.000060 0.000205
Cytoplasm 0.215050* 0.352569* 0.319711* 0.519890* 0.325321* 0.417948* 0.520524* 0.539187* 0.321246* 0.400991* 0.433686* 0.528900*
Cytoplasmic Foci 0.062743 0.231666* 0.005517 0.028676 0.130890 0.195778* 0.004794 0.025373 0.128372 0.205085* 0.005107 0.027134
Eisosomes 0.000145 0.000119 0.000017 0.000039 0.000154 0.000153 0.000014 0.000046 0.000154 0.000145 0.000015 0.000043
Endoplasmic Reticulum 0.002391 0.000800 0.001860 0.001401 0.001881 0.000421 0.000720 0.001229 0.001900 0.000519 0.001213 0.001321
Endosome 0.024324 0.047172 0.000974 0.006131 0.059384 0.046713 0.000840 0.006234 0.058089 0.046832 0.000898 0.006179
Golgi 0.013200 0.001707 0.000034 0.002478 0.002473 0.003615 0.000039 0.001651 0.002870 0.003120 0.000037 0.002092
Lipid Particles 0.004038 0.006133 0.000583 0.001044 0.008044 0.006402 0.000394 0.001233 0.007896 0.006332 0.000476 0.001132
Mitochondria 0.012970 0.003596 0.000788 0.001669 0.005583 0.006196 0.000773 0.003257 0.005856 0.005521 0.000780 0.002411
Mitotic Spindle 0.048710 0.002249 0.003901 0.020781 0.000462 0.005424 0.006096 0.011418 0.002245 0.004601 0.005147 0.016409
None 0.000553 0.000864 0.004005 0.012961 0.000602 0.001710 0.004713 0.004824 0.000600 0.001490 0.004407 0.009162
Nuclear Periphery 0.000796 0.000460 0.002179 0.002456 0.000346 0.000301 0.001716 0.001170 0.000363 0.000342 0.001916 0.001856
Nuclear Periphery Foci 0.001398 0.000859 0.000301 0.001568 0.000193 0.000334 0.000224 0.000517 0.000238 0.000470 0.000258 0.001078
Nucleolus 0.000264 0.001188 0.000382 0.001117 0.000992 0.001498 0.000516 0.000572 0.000965 0.001418 0.000458 0.000863
Nucleus 0.138493* 0.078070 0.622576* 0.248854* 0.060601 0.083786 0.410914* 0.266084* 0.063479 0.082303 0.502444* 0.256899*
Peroxisomes 0.001515 0.011942 0.000292 0.000768 0.004251 0.010786 0.000289 0.000898 0.004150 0.011086 0.000290 0.000829
Vacuole 0.383571* 0.218190* 0.023073 0.127093 0.357572* 0.171885 0.033146 0.100776 0.358532* 0.183894 0.028790 0.114805
Vacuole Periphery 0.019531 0.003100 0.000171 0.001486 0.003405 0.002297 0.000133 0.002270 0.004001 0.002506 0.000149 0.001852

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 2.06 2.24 2.12 0.31 -1.61 -3.17 -0.04 -3.32 -0.65 -3.25 -2.20 0.47 -2.64 -0.20 -3.72
Bud Neck -7.89 -5.03 -5.34 6.89 0.75 -1.62 16.69 12.30 10.75 -1.58 -1.46 17.36 16.08 13.90 -0.83
Bud Site 1.78 2.16 1.97 0.92 -1.39 -2.20 2.46 -3.06 -1.65 -4.31 -1.54 2.73 -1.81 -0.47 -4.15
Cell Periphery 2.06 2.36 1.61 -0.87 -1.66 0.42 12.52 -1.23 -1.35 -4.30 1.40 9.42 -0.77 -1.32 -3.54
Cytoplasm -6.11 -4.14 -10.11 -5.79 -15.22 -9.73 -13.96 -11.39 -1.90 -1.12 -9.44 -7.48 -14.95 -4.21 -9.95
Cytoplasmic Foci -16.02 4.93 2.58 23.10 -12.94 -11.45 42.53 33.35 34.20 -12.99 -14.94 42.53 34.70 41.37 -18.43
Eisosomes 0.06 2.76 2.07 9.37 -14.10 0.05 12.17 8.64 12.72 -13.58 0.67 12.38 9.44 15.95 -19.59
Endoplasmic Reticulum 2.54 -0.57 0.67 -5.40 3.64 5.73 1.84 -0.22 -11.84 -4.10 5.55 0.03 0.04 -13.50 0.03
Endosome -5.87 3.19 2.40 11.42 -6.23 4.15 30.70* 27.19 16.54 -4.70 4.04 30.74 28.25 20.18 -7.60
Golgi 1.05 1.40 1.17 0.95 -1.85 -1.64 9.52 3.45 3.71 -1.80 -0.83 8.54 3.63 3.64 -2.54
Lipid Particles -1.75 2.23 1.78 3.82 -6.99 1.92 14.91 12.92 7.39 -9.61 1.96 14.93 13.42 8.41 -12.20
Mitochondria 0.55 1.03 1.00 1.66 -0.84 -0.44 5.50 3.66 3.84 -1.85 0.13 5.59 4.68 4.68 -2.04
Mitotic Spindle 1.87 1.72 0.77 -5.98 -5.16 -3.60 -6.33 -5.40 -2.26 -2.19 -2.55 -4.52 -7.67 -5.30 -5.40
None -1.16 -12.65 -6.30 -6.10 -3.58 -3.41 -24.35* -11.29 -6.23 0.48 -3.54 -24.97 -8.96 -7.20 -3.19
Nuclear Periphery 3.54 -7.26 -3.83 -5.97 -0.45 1.05 -31.67 -17.52 -18.66 4.82 0.77 -37.50 -10.09 -10.31 0.92
Nuclear Periphery Foci 1.07 2.94 -0.33 -1.44 -3.84 -0.92 -3.41 -7.00 -2.46 -4.30 -1.51 -4.05 -6.23 -3.16 -5.04
Nucleolus -5.77 -3.52 -3.52 0.73 -2.73 -2.37 3.13 2.43 3.84 -1.31 -2.56 4.16 0.81 3.07 -2.83
Nucleus 3.02 -18.27 -4.69 -24.38 41.16 -6.04 -79.51* -33.82 -28.84 17.77 -5.23 -108.27* -44.63 -38.45 39.70
Peroxisomes -5.31 1.72 1.40 5.56 -0.68 -5.61 10.56 8.54 9.00 -2.47 -6.67 10.31 9.36 10.67 -2.22
Vacuole 6.76 11.96 7.26 1.19 -25.34 24.66* 49.19* 23.05 0.53 -16.01 24.82 54.48* 24.52 -0.53 -28.81
Vacuole Periphery 1.16 1.66 1.57 2.16 -2.51 2.99 18.20 5.00 2.19 -2.33 2.52 12.55 7.70 3.68 -3.28
Standard name
Human Ortholog
Description N-acetylglucosaminyltransferase; capable of modification of N-linked glycans in the Golgi apparatus
Localization
Cell Percentages vacuole (69%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Gnt1

Gnt1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gnt1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available