Standard name
Human Ortholog
Description Alpha-arrestin involved in ubiquitin-dependent endocytosis; regulates endocytosis of plasma membrane proteins by recruiting the ubiquitin ligase Rsp5p to its targets; involved in the basal internalization and turnover of alpha-factor receptor Ste2p; recruits ubiquitin ligase Rsp5p to Ste2p via its 2 PPXY motifs; inhibited by Npr1p-mediated phosphorylation, which affects translocation between the cytosol and the plasma membrane
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.15 0.11 0.12 0 0 0 0 0 0 0 0 0 0.07 0.07 0 0.06 0.14 0.07 0.12 0.14 0.09 0.05 0 0
Bud 0 0.05 0.06 0 0 0.07 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.09 0.07 0.06 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0.07 0.05 0 0 0 0 0 0 0 0 0 0.09 0.17 0.14 0.08 0.12 0.15 0 0 0 0 0 0
Cytoplasm 0.73 0.72 0.78 0.97 0.92 0.88 0.9 0.9 0.82 0.8 0.57 0.8 0.82 0.89 0.91 0.77 0.72 0.82 0.51 0.47 0.49 0.55 0.64 0.7
Endoplasmic Reticulum 0.05 0.15 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0.05 0 0 0.13 0.07 0.06 0.16 0.12 0.11
Endosome 0.09 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0.09 0.08 0.05 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.21 0.1 0.06 0.23 0.28 0.52 0.24 0 0 0 0 0 0 0 0 0.13 0 0 0
Nucleus 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 19 40 9 0 0 0 3 0 1 0 0 0 11 21 12 9 15 8 18 61 9 7 10 9
Bud 1 18 5 1 3 3 7 1 2 5 0 2 6 9 4 0 2 0 1 10 0 4 3 2
Bud Neck 0 1 1 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 8 42 7 9 8 8
Bud Site 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 5 24 4 0 0 0 2 3 4 2 0 1 14 53 44 12 13 16 1 6 1 2 4 4
Cytoplasm 93 256 61 32 119 37 128 111 117 93 12 47 127 272 285 113 79 90 78 213 53 86 162 196
Endoplasmic Reticulum 7 55 1 0 1 0 3 2 0 1 0 0 12 6 11 7 4 4 20 32 6 25 31 29
Endosome 12 4 5 1 5 0 1 1 1 0 0 2 2 9 9 9 3 2 13 36 5 3 5 8
Golgi 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 3 0 4 5 1 0 1 1
Mitochondria 1 4 0 0 1 9 14 7 32 33 11 14 1 1 1 3 0 0 1 13 14 4 7 4
Nucleus 0 2 1 0 2 0 3 8 1 4 0 1 0 0 1 0 1 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 0
Nucleolus 0 0 0 0 1 0 0 0 0 1 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0
SpindlePole 2 1 2 0 1 0 1 1 0 0 0 0 0 2 0 4 6 6 2 4 1 0 0 0
Vac/Vac Membrane 0 1 1 0 1 0 0 2 2 0 0 1 0 1 0 6 2 1 3 17 3 6 13 8
Unique Cell Count 128 357 78 33 129 42 142 124 142 116 21 59 154 306 314 147 109 110 155 451 110 158 254 280
Labelled Cell Count 141 407 90 34 135 49 162 136 161 139 23 68 173 376 368 164 130 127 155 451 110 158 254 280


Cytoplasm, Late Golgi

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.2 7.8 7.0 5.9 7.2 5.7 5.8 6.1 5.2 5.1 4.7 5.1 7.3 7.5 7.5 10.4 10.9 9.8 7.0 7.1 7.3
Std Deviation (1e-4) 0.8 0.9 1.1 0.8 1.5 1.6 1.3 1.6 1.2 1.4 0.7 0.9 0.9 1.0 1.1 1.4 1.5 1.3 1.0 1.1 1.0
Intensity Change (Log2) – – – -0.26 0.03 -0.31 -0.29 -0.22 -0.43 -0.46 -0.59 -0.46 0.04 0.08 0.08 0.56 0.63 0.48 -0.02 0.01 0.05

WT3RAP60RAP140RAP220RAP300RAP380RAP460RAP540RAP620RAP7000510WT3HU80HU120HU1600510WT3rpd3Δ_1rpd3Δ_2rpd3Δ_30510WT1AF100AF140AF1800510
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 -1.1 -1.4 0 -1.4 0.4 -1.0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 1.1 2.7 2.1 0.8 1.6 2.1
Cytoplasm 2.5 2.9 1.3 2.4 2.2 0.8 0.3 -1.9 0.2 0.8 2.5 3.1 -0.2 -0.9 0.6
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 4.5 5.2 0 4.5 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.2864 6.3748 5.7861 5.694 5.1097 5.8992 3.9443 5.0285 4.7145 4.3621 4.2743 4.6512 3.58 3.9617 3.8067 3.6433 3.4511 3.7962
Actin 0.0632 0.0469 0.0375 0.0091 0.0438 0.0397 0.0833 0.0503 0.0916 0.0596 0.0948 0.0807 0.0469 0.0239 0.0739 0.0135 0.0798 0.0792
Bud 0.0014 0.0017 0.0022 0.0003 0.001 0.0017 0.0015 0.0059 0.0028 0.0022 0.0017 0.0018 0.0006 0.0011 0.001 0.0006 0.0011 0.0007
Bud Neck 0.0137 0.0093 0.0162 0.0049 0.0719 0.0707 0.0068 0.0058 0.0152 0.021 0.0055 0.0752 0.0034 0.0038 0.0162 0.0586 0.0074 0.0524
Bud Periphery 0.0029 0.002 0.0053 0.0004 0.0034 0.0038 0.002 0.0027 0.0066 0.004 0.0022 0.0026 0.0012 0.0008 0.0013 0.0007 0.0013 0.0019
Bud Site 0.0271 0.1427 0.0366 0.0011 0.0046 0.0037 0.0158 0.097 0.0274 0.0194 0.0026 0.0032 0.0242 0.0985 0.0519 0.0025 0.0101 0.0012
Cell Periphery 0.0254 0.0206 0.0152 0.0022 0.0399 0.0109 0.0225 0.0131 0.0176 0.0091 0.0082 0.0103 0.0555 0.0164 0.0113 0.0057 0.0098 0.0133
Cytoplasm 0.3798 0.4407 0.4895 0.691 0.5909 0.521 0.4394 0.5329 0.4758 0.4589 0.7326 0.4919 0.4644 0.5089 0.579 0.5725 0.483 0.6067
Cytoplasmic Foci 0.1503 0.1312 0.1311 0.1993 0.1028 0.1629 0.1417 0.155 0.158 0.1284 0.0738 0.1464 0.1002 0.2004 0.0903 0.131 0.0616 0.0964
Eisosomes 0.0015 0.0004 0.0007 0 0.0006 0.0003 0.0011 0.0004 0.0004 0.0002 0.0002 0.0003 0.0004 0.0001 0.0004 0 0.0001 0.0003
Endoplasmic Reticulum 0.0168 0.0082 0.0118 0.0042 0.0197 0.0138 0.0152 0.0076 0.0108 0.0143 0.0067 0.01 0.0288 0.0066 0.0178 0.0065 0.0719 0.028
Endosome 0.1061 0.0665 0.1055 0.0241 0.0543 0.0544 0.0862 0.0485 0.0675 0.1179 0.0279 0.056 0.0547 0.0431 0.0465 0.1267 0.0451 0.0454
Golgi 0.0553 0.0393 0.0445 0.0103 0.025 0.0437 0.0549 0.0383 0.0468 0.0593 0.0279 0.0588 0.0264 0.0216 0.0273 0.0553 0.0135 0.0304
Lipid Particles 0.0645 0.0415 0.0378 0.0045 0.0094 0.0243 0.0744 0.0271 0.047 0.0272 0.0082 0.0344 0.0949 0.038 0.0212 0.0136 0.02 0.022
Mitochondria 0.0193 0.0029 0.0045 0.0003 0.0018 0.0088 0.0093 0.0007 0.0026 0.0398 0.0014 0.0099 0.0013 0.0004 0.001 0.0007 0.0018 0.0012
None 0.0397 0.0196 0.0302 0.0024 0.0071 0.0087 0.0141 0.0025 0.0067 0.0046 0.0012 0.0022 0.0631 0.0045 0.0306 0.001 0.0033 0.0026
Nuclear Periphery 0.0026 0.0006 0.0019 0.0003 0.0081 0.0014 0.0064 0.0003 0.0005 0.0037 0.0002 0.0004 0.0056 0.0004 0.0145 0.0004 0.1422 0.0094
Nucleolus 0.0009 0.0004 0.0004 0 0.0003 0.0001 0.0007 0.0002 0.0002 0.0002 0 0.0001 0.0002 0.0005 0.0002 0.0001 0.0007 0.0001
Nucleus 0.0011 0.0008 0.0012 0.0004 0.0017 0.0032 0.0023 0.0005 0.0006 0.0008 0.0003 0.0004 0.0013 0.0009 0.0046 0.0003 0.0278 0.0006
Peroxisomes 0.0169 0.0119 0.0151 0.0422 0.0022 0.0202 0.008 0.0064 0.0152 0.0151 0.0026 0.0108 0.0058 0.0171 0.0033 0.0024 0.0022 0.0044
Punctate Nuclear 0.0019 0.0009 0.0022 0.0016 0.0018 0.0008 0.0071 0.0007 0.0009 0.0008 0.0003 0.0007 0.0015 0.0012 0.0017 0.0006 0.0072 0.0007
Vacuole 0.0083 0.0105 0.0096 0.0013 0.0089 0.0054 0.0064 0.0039 0.0051 0.0101 0.0015 0.0033 0.019 0.0114 0.0055 0.0066 0.0056 0.0028
Vacuole Periphery 0.0015 0.0014 0.001 0.0001 0.0009 0.0006 0.001 0.0002 0.0006 0.0036 0.0002 0.0006 0.0008 0.0003 0.0006 0.0006 0.0046 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 20.658 17.2626 14.8949 22.4528 19.7968 15.2605 21.4118 23.4238 21.9515 22.5147
Translational Efficiency 0.5624 0.7401 0.8193 0.5365 0.5658 0.9066 0.6246 0.6476 0.5836 0.5854

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
954 1593 982 1268 1802 1974 209 1432 2756 3567 1191 2700

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 799.63 833.00 1067.88 923.76 852.44 871.80 1077.62 936.62 834.16 854.47 1069.59 930.58
Standard Deviation 85.07 137.72 131.54 117.94 98.33 105.69 104.63 117.70 97.25 122.57 127.28 117.98
Intensity Change Log 2 — 0.058984 0.417345 0.208185 — 0.032399 0.338178 0.135866 — 0.045328 0.377039 0.171323

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.009731 0.013681 0.055055 0.006278 0.018424 0.006677 0.060114 0.011669 0.015415 0.009805 0.055942 0.009137
Bud Neck 0.068408 0.021828 0.004429 0.011026 0.029829 0.035218 0.010807 0.010266 0.043183 0.029238 0.005549 0.010623
Bud Site 0.065533 0.042566 0.037548 0.054323 0.065942 0.040693 0.071521 0.069949 0.065800 0.041529 0.043509 0.062611
Cell Periphery 0.007087 0.000362 0.000186 0.000188 0.001593 0.000686 0.000798 0.000237 0.003495 0.000541 0.000293 0.000214
Cytoplasm 0.432479 0.253660 0.024156 0.265631 0.342324 0.282731 0.089599 0.287058 0.373531 0.269748 0.035640 0.276995
Cytoplasmic Foci 0.208700 0.390400 0.360520 0.209663 0.318935 0.338425 0.330064 0.203092 0.280777 0.361637 0.355175 0.206178
Eisosomes 0.009942 0.001250 0.000163 0.000183 0.003663 0.002041 0.000739 0.000237 0.005836 0.001688 0.000264 0.000212
Endoplasmic Reticulum 0.004173 0.001825 0.004949 0.001286 0.003840 0.001471 0.004223 0.001633 0.003956 0.001629 0.004822 0.001470
Endosome 0.037712 0.079409 0.156377 0.055630 0.042156 0.088569 0.092211 0.040188 0.040618 0.084478 0.145117 0.047440
Golgi 0.064388 0.073110 0.078358 0.007454 0.089130 0.070780 0.010952 0.008469 0.080565 0.071821 0.066529 0.007992
Lipid Particles 0.015269 0.014947 0.055611 0.006791 0.018380 0.014342 0.026868 0.007157 0.017303 0.014612 0.050567 0.006985
Mitochondria 0.003668 0.005522 0.000316 0.003766 0.004032 0.007823 0.000059 0.003029 0.003906 0.006796 0.000271 0.003375
Mitotic Spindle 0.000571 0.004567 0.126084 0.027289 0.000725 0.003660 0.007048 0.040032 0.000672 0.004065 0.105195 0.034048
None 0.000399 0.001261 0.000026 0.000438 0.000432 0.000275 0.000063 0.001393 0.000421 0.000715 0.000033 0.000945
Nuclear Periphery 0.000047 0.000075 0.000246 0.000361 0.000026 0.000048 0.000163 0.000422 0.000033 0.000060 0.000231 0.000394
Nuclear Periphery Foci 0.000585 0.000713 0.009325 0.002511 0.000585 0.000363 0.008412 0.002504 0.000585 0.000519 0.009165 0.002507
Nucleolus 0.000117 0.001196 0.000043 0.000455 0.000138 0.000233 0.000080 0.000271 0.000130 0.000663 0.000049 0.000357
Nucleus 0.006641 0.006441 0.000934 0.020921 0.005776 0.006598 0.005520 0.015084 0.006075 0.006528 0.001738 0.017825
Peroxisomes 0.021855 0.032198 0.047105 0.055450 0.022120 0.028096 0.077934 0.057995 0.022028 0.029928 0.052515 0.056800
Vacuole 0.041209 0.051921 0.037893 0.267813 0.030247 0.068122 0.202501 0.236945 0.034042 0.060886 0.066779 0.251442
Vacuole Periphery 0.001487 0.003069 0.000677 0.002542 0.001702 0.003149 0.000325 0.002371 0.001628 0.003113 0.000615 0.002451

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.23 -10.51 5.58 5.62 11.90 7.32 -3.67 6.03 -0.74 4.82 4.48 -9.79 7.94 3.70 12.45
Bud Neck 12.11 17.18 15.49 8.37 -6.87 -2.62 10.57 14.01 16.86 0.74 7.47 22.60 20.15 18.41 -7.13
Bud Site 5.76 6.03 5.31 0.33 -0.47 8.58 -0.39 3.77 -3.00 2.58 10.30 6.93 6.61 -2.04 -0.64
Cell Periphery 8.21 8.41 8.47 7.31 2.57 4.74 5.18 11.98 3.30 5.82 9.54 10.70 11.17 4.37 5.63
Cytoplasm 17.11 45.70 14.76 -0.97 -28.60 7.31 22.49 6.03 -0.29 -16.13 16.11 58.44 13.56 -0.78 -38.89
Cytoplasmic Foci -22.37 -15.22 4.67 28.82 20.31 -2.85 0.05 22.51 25.40 10.18 -15.23 -8.43 21.32 38.03 24.19
Eisosomes 10.58 11.99 12.01 10.42 0.81 5.18 12.41 15.44 8.45 6.32 11.92 17.23 17.53 11.62 4.50
Endoplasmic Reticulum 2.24 -0.83 2.80 1.95 4.27 4.86 -0.81 4.97 0.15 4.99 4.81 -1.33 5.48 1.41 4.83
Endosome -11.30 -21.74 -0.53 10.54 21.11 -14.12 -4.81 5.09 17.76 6.23 -17.94 -22.27 3.16 20.02 23.53
Golgi -1.91 -1.87 18.09 23.20 19.13 4.88 24.11 28.76 24.35 2.48 3.18 5.01 33.89 33.62 18.91
Lipid Particles 0.15 -11.73 6.66 8.43 15.43 2.79 -1.97 9.70 7.38 5.45 2.43 -11.63 12.02 11.04 16.12
Mitochondria -2.31 7.03 1.53 3.14 -2.22 -4.28 11.06 3.39 6.16 -3.41 -4.77 12.42 3.36 6.64 -3.56
Mitotic Spindle -4.13 -17.01 -9.43 -7.87 12.02 -3.50 -2.49 -10.23 -9.09 -6.59 -5.34 -16.76 -13.89 -12.03 10.95
None -1.50 2.70 -1.08 1.23 -7.09 1.67 4.94 -1.64 -2.03 -2.63 -1.16 5.90 -1.92 -0.34 -3.87
Nuclear Periphery -1.58 -6.42 -15.30 -14.23 -6.12 -3.17 -6.47 -9.17 -8.76 -5.43 -3.19 -7.77 -15.86 -14.93 -6.30
Nuclear Periphery Foci -0.77 -6.28 -7.80 -8.08 4.48 1.18 -7.01 -7.58 -7.57 4.91 0.28 -7.37 -11.14 -10.99 5.36
Nucleolus -2.58 6.29 -4.60 0.84 -4.97 -2.28 4.34 -7.23 -2.00 -8.78 -2.85 8.83 -5.94 0.49 -6.84
Nucleus 0.12 10.08 -12.66 -12.42 -15.73 -1.58 -0.96 -13.38 -12.02 -9.39 -1.29 8.84 -18.35 -17.20 -21.15
Peroxisomes -4.55 -7.16 -5.09 -2.00 1.81 -3.92 -6.54 -6.50 -4.33 3.79 -6.12 -9.68 -8.26 -4.52 3.11
Vacuole -3.56 -1.73 -36.82 -35.38 -34.33 -14.08 -12.73 -40.09 -33.76 -5.58 -13.44 -10.11 -54.82 -48.74 -36.75
Vacuole Periphery -5.45 5.94 0.07 3.41 -2.01 -5.81 9.55 0.75 4.01 -2.80 -7.69 8.37 0.70 5.27 -2.91
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Alpha-arrestin involved in ubiquitin-dependent endocytosis; regulates endocytosis of plasma membrane proteins by recruiting the ubiquitin ligase Rsp5p to its targets; involved in the basal internalization and turnover of alpha-factor receptor Ste2p; recruits ubiquitin ligase Rsp5p to Ste2p via its 2 PPXY motifs; inhibited by Npr1p-mediated phosphorylation, which affects translocation between the cytosol and the plasma membrane
Localization
Cell Percentages cytoplasm (67%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-10

Ldb19

Ldb19


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ldb19-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available