Standard name
Human Ortholog
Description Type V myosin motor involved in actin-based transport of cargos; required for the polarized delivery of secretory vesicles, the vacuole, late Golgi elements, peroxisomes, and the mitotic spindle; MYO2 has a paralog, MYO4, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.24 0.28 0.28 0.25 0.24 0.19 0.13 0.12 0.09 – 0.12 0.1 0.17 0.12 0.1 0.13 0.11 0.14 0.19 0.13 0.12 0.21 0.19 0.12
Bud 0 0.12 0.2 0.19 0.23 0.15 0.13 0.11 0.16 – 0.17 0.14 0 0.05 0.07 0 0 0.05 0.17 0.24 0.17 0.35 0.33 0.23
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0.08 0.08 0.09 0.07 0
Bud Site 0 0 0.07 0.09 0.05 0.1 0 0 0 – 0 0.05 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.07 0 0.05 0.05 0 0 0.05 0 0 – 0.05 0.06 0.08 0 0.1 0.06 0 0.06 0 0 0 0 0 0
Cytoplasm 0.52 0.53 0.45 0.41 0.38 0.4 0.4 0.39 0.44 – 0.33 0.34 0.65 0.71 0.73 0.6 0.51 0.55 0.4 0.39 0.34 0.18 0.2 0.39
Endoplasmic Reticulum 0.1 0.12 0.08 0.08 0 0.05 0.05 0.05 0 – 0 0.07 0.12 0.12 0.09 0.07 0 0.05 0.09 0 0.07 0 0 0.11
Endosome 0 0 0.05 0.06 0.07 0.1 0.07 0.07 0.05 – 0 0.06 0 0 0 0 0 0 0 0 0 0.06 0.05 0
Golgi 0.09 0 0 0 0 0 0 0 0 – 0 0 0.05 0 0 0.14 0.15 0.15 0 0 0 0 0 0
Mitochondria 0 0 0 0.06 0 0.1 0.11 0.11 0.27 – 0.41 0.28 0 0 0 0.05 0 0.05 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0.09 0.05 0.09 0.06 – 0.06 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.06 0.06 0.11 0.05 0.05 0 0 – 0 0.05 0 0 0 0 0 0 0 0 0.05 0 0.05 0
Vac/Vac Membrane 0.06 0 0.06 0.07 0.11 0.05 0.18 0.2 0.06 – 0.08 0.1 0 0 0 0.11 0.23 0.15 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 30 77 76 71 90 68 71 52 30 – 39 48 54 42 38 16 16 15 30 44 40 63 102 61
Bud 5 34 54 52 86 55 69 46 56 – 56 65 9 18 28 4 6 5 27 81 55 106 177 121
Bud Neck 0 0 1 0 4 1 1 3 2 – 0 0 0 0 0 0 0 0 7 26 26 26 37 12
Bud Site 2 7 18 25 18 35 11 14 13 – 9 25 5 5 2 0 2 1 – – – – – –
Cell Periphery 9 6 14 13 11 15 25 14 15 – 18 27 25 16 38 7 6 7 3 0 1 0 0 0
Cytoplasm 65 146 123 114 142 146 210 170 150 – 111 163 213 258 285 73 74 60 64 135 110 54 108 202
Endoplasmic Reticulum 12 34 21 22 14 20 25 20 14 – 13 34 40 45 36 9 6 5 15 13 23 6 22 58
Endosome 2 2 15 18 26 35 37 29 17 – 13 29 2 2 6 2 0 1 2 9 14 18 26 17
Golgi 11 12 1 2 6 1 5 6 0 – 1 2 15 8 8 17 22 16 0 1 2 1 1 2
Mitochondria 0 4 5 17 17 37 56 48 93 – 137 135 3 6 16 6 0 5 1 2 13 2 5 2
Nucleus 0 3 0 0 0 0 7 7 2 – 3 6 0 1 2 1 1 0 0 2 0 0 2 1
Nuclear Periphery 0 0 0 1 0 0 1 0 1 – 1 3 0 0 0 0 0 0 0 0 0 0 1 0
Nucleolus 0 8 8 8 14 32 24 39 20 – 20 25 1 2 1 0 3 1 0 1 0 1 0 0
Peroxisomes 0 2 8 7 16 13 13 4 6 – 2 14 3 2 1 0 0 0 0 0 2 2 6 1
SpindlePole 2 2 16 16 40 18 28 16 10 – 2 25 4 4 1 2 2 3 3 6 16 11 26 17
Vac/Vac Membrane 7 9 16 20 43 18 97 86 22 – 27 47 6 9 7 13 33 16 1 11 13 5 10 13
Unique Cell Count 125 278 273 281 378 365 529 433 340 336 478 326 361 393 122 145 110 161 343 329 306 535 519
Labelled Cell Count 145 346 376 386 527 494 680 554 451 452 648 380 418 469 150 171 135 161 343 329 306 535 519


Ambiguous, Bud, Bud Neck, Cell Periphery, Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 17.6 14.8 10.2 10.2 10.9 9.5 10.0 9.4 8.2 – 8.0 7.9 14.8 14.2 12.7 16.9 18.7 19.2 15.4 13.5 14.5
Std Deviation (1e-4) 3.2 3.0 1.6 1.9 1.9 2.0 2.1 2.6 1.8 – 1.8 1.8 2.4 3.3 3.1 4.7 4.4 4.5 2.7 2.5 2.4
Intensity Change (Log2) – – – 0.01 0.11 -0.09 -0.03 -0.11 -0.3 – -0.34 -0.37 0.54 0.48 0.32 0.74 0.88 0.92 0.59 0.41 0.51


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -0.7 -1.2 -2.8 -5.0 -5.3 -6.2 – -5.1 -6.3 -3.3 -5.2 -6.1 -3.2 -3.9 -3.0
Bud -0.4 0.9 -1.6 -2.5 -3.4 -1.1 – -1.0 -2.2 -6.8 -5.8 -4.9 -4.3 -4.3 -3.7
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 1.0 -1.0 1.4 -3.2 -2.1 -1.6 – -2.3 -0.8 -3.2 -3.5 -4.5 -2.9 -2.4 -2.3
Cell Periphery -0.3 -1.5 -0.6 -0.3 -1.3 -0.4 – 0.1 0.3 1.3 -0.4 2.1 0.2 -0.5 0.5
Cytoplasm -1.1 -1.9 -1.3 -1.5 -1.5 -0.2 – -3.0 -3.0 5.0 6.7 7.2 2.7 1.2 1.7
Endoplasmic Reticulum 0.1 -2.2 -1.1 -1.7 -1.7 -1.9 – -2.0 -0.3 1.8 1.9 0.7 -0.1 -1.4 -1.1
Endosome 0.5 0.7 1.9 0.8 0.6 -0.3 – -1.0 0.3 -3.6 -3.8 -2.9 -1.7 -2.9 0
Golgi 0 0 0 0 0 0 – 0 0 3.2 0 0 6.0 6.3 0
Mitochondria 2.5 1.9 4.2 4.4 4.5 8.6 – 11.3 8.9 0 0 1.6 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus -0.1 0.5 3.0 1.1 3.2 1.7 – 1.8 1.5 0 0 0 0 0 0
Peroxisomes -0.3 0.9 0.4 -0.4 0 -1.0 – 0 0 -1.8 0 0 0 0 0
SpindlePole -0.1 2.1 -0.5 -0.3 -1.3 -1.8 – -3.8 -0.4 -3.1 -3.4 -4.5 -1.9 -2.1 -1.3
Vacuole 0.6 2.4 -0.5 4.8 5.2 0.3 – 1.0 1.9 -2.6 -2.2 -2.8 1.7 5.1 2.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 14.7559 16.9371 16.331 15.0769 15.8035 17.2575 11.2716 15.5909 15.7748 15.7911 14.5967 15.6307 11.6326 14.5428 13.6332 14.2668 11.6925 14.0644
Actin 0.0911 0.0283 0.0499 0.0633 0.052 0.0431 0.0824 0.0383 0.0639 0.0155 0.0291 0.0775 0.1069 0.0409 0.073 0.0145 0.1114 0.1315
Bud 0.04 0.039 0.2084 0.1062 0.1104 0.0136 0.0292 0.0423 0.1847 0.0494 0.1004 0.0216 0.0558 0.0621 0.2005 0.1026 0.0719 0.029
Bud Neck 0.0551 0.0194 0.0088 0.0125 0.0087 0.5538 0.0436 0.0101 0.0041 0.0025 0.0098 0.5924 0.0348 0.0026 0.0034 0.006 0.057 0.4683
Bud Periphery 0.0575 0.0168 0.2139 0.111 0.1452 0.0257 0.0223 0.0158 0.1268 0.0711 0.0665 0.0194 0.0555 0.0253 0.1691 0.0729 0.0783 0.0307
Bud Site 0.1574 0.3842 0.0806 0.0774 0.0142 0.0175 0.1999 0.457 0.0786 0.0052 0.051 0.0098 0.174 0.3623 0.0998 0.0172 0.0167 0.0248
Cell Periphery 0.0246 0.0065 0.0066 0.0146 0.0117 0.0073 0.005 0.0026 0.0024 0.0013 0.0018 0.0022 0.0104 0.0037 0.0034 0.0037 0.0022 0.0041
Cytoplasm 0.2426 0.3406 0.3174 0.3397 0.4313 0.1914 0.2403 0.347 0.4265 0.7556 0.5691 0.1794 0.3587 0.4584 0.3734 0.6512 0.3875 0.1769
Cytoplasmic Foci 0.0885 0.0696 0.0252 0.0491 0.023 0.0753 0.1974 0.0569 0.0342 0.0132 0.0418 0.0599 0.0521 0.0203 0.0196 0.0098 0.0373 0.0632
Eisosomes 0.0007 0.0001 0.0001 0.0002 0.0002 0.0001 0.0012 0.0001 0.0002 0.0001 0.0001 0.0001 0.0005 0.0001 0.0001 0 0.0005 0.0002
Endoplasmic Reticulum 0.0335 0.0163 0.0094 0.0203 0.0105 0.0145 0.02 0.002 0.0119 0.0023 0.0042 0.0024 0.0196 0.007 0.0062 0.0045 0.0209 0.0055
Endosome 0.0456 0.0183 0.0168 0.0281 0.041 0.0141 0.0333 0.004 0.0157 0.0207 0.0462 0.0142 0.0317 0.0052 0.0168 0.0377 0.0606 0.0179
Golgi 0.0284 0.0073 0.0047 0.0123 0.0087 0.0093 0.0126 0.0036 0.0091 0.0212 0.021 0.0081 0.0197 0.0024 0.007 0.0029 0.0256 0.0167
Lipid Particles 0.0211 0.002 0.0025 0.0143 0.0228 0.0019 0.0107 0.0016 0.0054 0.0011 0.0035 0.0013 0.0175 0.0007 0.0011 0.0004 0.0191 0.0022
Mitochondria 0.0276 0.0013 0.01 0.0537 0.0104 0.009 0.0068 0.0014 0.0031 0.0105 0.0125 0.0017 0.0221 0.0003 0.0021 0.0013 0.0198 0.0058
None 0.0071 0.0011 0.001 0.0036 0.0009 0.0004 0.0099 0.0009 0.0033 0.0009 0.0052 0.0004 0.002 0.0008 0.0037 0.0014 0.0335 0.0004
Nuclear Periphery 0.0081 0.0011 0.0056 0.0061 0.0018 0.0012 0.0151 0.0006 0.0035 0.001 0.0016 0.0003 0.0028 0.0005 0.0028 0.0028 0.0085 0.0006
Nucleolus 0.0019 0.0011 0.0003 0.0029 0.0032 0.0007 0.0023 0.0006 0.0004 0.0002 0.0028 0.0004 0.0005 0.0002 0.0006 0.0002 0.0061 0.0003
Nucleus 0.0049 0.0033 0.0026 0.0067 0.006 0.0025 0.009 0.0019 0.0091 0.0025 0.0041 0.0009 0.0021 0.0013 0.0019 0.004 0.005 0.0015
Peroxisomes 0.015 0.0034 0.0019 0.0081 0.0031 0.0052 0.0144 0.0052 0.0052 0.0004 0.0025 0.0041 0.012 0.0007 0.001 0.0004 0.0101 0.009
Punctate Nuclear 0.0099 0.0009 0.0018 0.0021 0.0006 0.0019 0.0121 0.0008 0.0013 0.0005 0.0015 0.0008 0.0013 0.0006 0.0022 0.0005 0.0077 0.0006
Vacuole 0.0344 0.0376 0.0274 0.0561 0.0848 0.0098 0.0276 0.0068 0.0092 0.0216 0.0221 0.0026 0.0179 0.0041 0.0106 0.0506 0.0158 0.009
Vacuole Periphery 0.0051 0.0016 0.0051 0.0115 0.0094 0.0017 0.0048 0.0006 0.0015 0.0032 0.0034 0.0006 0.0022 0.0004 0.0018 0.0154 0.0046 0.0018

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 114.4271 112.5199 65.6804 76.2208 90.0756 90.5574 109.5473 99.7224 77.2263 106.7595
Translational Efficiency 0.6152 0.585 0.6318 0.5026 0.5333 0.6464 0.5382 0.4545 0.5192 0.4972

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✔
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Type V myosin motor involved in actin-based transport of cargos; required for the polarized delivery of secretory vesicles, the vacuole, late Golgi elements, peroxisomes, and the mitotic spindle; MYO2 has a paralog, MYO4, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (84%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-5

Myo2

Myo2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Myo2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available