Standard name
Human Ortholog
Description Mitochondrial inner membrane Mg(2+) channel; required for maintenance of intramitochondrial Mg(2+) concentrations at the correct level to support splicing of group II introns; similar to bacterial CorA
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0 0 – 0.05 – 0 0 0 0 0 0.05
Bud 0 0 0.05 0.05 0 0 0.05 0 0.07 0.05 0.08 0 0 0 0 – 0.19 – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0.05 – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0.05 – 0 0 0 0 0 0
Cytoplasm 0.18 0.29 0.16 0.16 0.19 0.18 0.15 0.15 0.11 0.1 0.12 0.05 0.71 0.85 0.87 – 0.38 – 0 0 0 0.05 0 0
Endoplasmic Reticulum 0.06 0 0 0 0 0 0 0 0 0 0 0 0.09 0 0.05 – 0.05 – 0 0 0 0 0 0
Endosome 0.07 0 0.16 0.08 0.36 0.08 0.06 0 0 0 0 0 0 0 0 – 0.24 – 0.23 0.11 0.18 0.12 0.11 0.11
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Mitochondria 0.75 0.66 0.53 0.68 0.47 0.7 0.76 0.75 0.88 0.87 0.9 0.91 0 0 0 – 0.14 – 0.48 0.63 0.53 0.61 0.6 0.57
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Peroxisomes 0 0 0.26 0.18 0.15 0.06 0.07 0.06 0 0 0 0 0 0 0 – 0 – 0 0 0.12 0 0 0.05
SpindlePole 0 0 0.11 0.08 0.08 0.09 0.12 0.15 0.1 0.12 0.09 0.12 0.06 0 0 – 0.05 – 0.06 0 0.05 0.05 0.05 0
Vac/Vac Membrane 0 0.05 0 0 0.12 0.07 0 0 0 0 0 0 0 0.05 0 – 0.05 – 0.06 0.05 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 7 0 0 0 0 0 0 0 0 0 0 9 4 7 – 1 – 1 8 9 7 14 27
Bud 1 7 13 12 6 9 23 26 28 21 38 23 1 3 0 – 4 – 0 2 1 2 6 8
Bud Neck 0 0 0 0 0 1 0 3 2 0 2 0 4 7 4 – 1 – 1 3 1 4 10 14
Bud Site 1 0 0 0 0 2 0 14 8 7 17 8 0 0 0 – 0 – – – – – – –
Cell Periphery 0 2 6 2 0 0 0 1 0 1 2 5 0 4 2 – 1 – 0 0 0 0 1 2
Cytoplasm 18 62 43 36 39 65 62 91 40 43 58 25 72 171 230 – 8 – 2 10 8 12 15 12
Endoplasmic Reticulum 6 7 4 2 0 1 0 2 1 0 0 7 9 3 13 – 1 – 1 0 1 0 4 5
Endosome 7 6 43 18 73 29 26 24 6 7 0 7 1 3 3 – 5 – 28 26 62 32 53 59
Golgi 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 – 0 – 2 2 6 1 6 6
Mitochondria 76 140 145 157 95 251 319 442 333 376 435 487 0 4 0 – 3 – 59 148 186 162 295 300
Nucleus 0 0 0 0 0 3 3 10 5 7 5 2 1 2 4 – 0 – 0 1 0 0 0 2
Nuclear Periphery 1 2 0 0 0 0 3 2 4 0 0 5 0 0 0 – 0 – 2 1 1 1 3 2
Nucleolus 2 0 0 1 0 2 1 8 2 8 4 1 0 0 0 – 0 – 2 0 0 1 3 3
Peroxisomes 3 2 72 42 31 23 29 38 17 15 10 23 0 0 0 – 0 – 2 6 40 9 19 24
SpindlePole 4 3 30 19 17 34 50 87 39 50 44 62 6 2 4 – 1 – 7 7 15 12 25 20
Vac/Vac Membrane 3 10 9 4 24 25 16 14 7 4 3 6 1 10 4 – 1 – 6 12 4 10 21 23
Unique Cell Count 101 212 275 231 203 358 422 591 380 434 485 537 101 202 263 21 124 238 353 266 490 527
Labelled Cell Count 122 248 366 294 285 445 532 762 492 539 618 661 104 213 271 26 124 238 353 266 490 527


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.7 4.1 4.0 4.0 4.2 3.4 3.2 3.3 3.1 2.8 2.9 2.9 4.9 5.0 5.2 – 5.0 – 5.2 5.4 5.6
Std Deviation (1e-4) 0.4 0.8 1.0 1.6 2.1 1.2 1.6 1.3 1.4 1.2 1.1 0.8 0.5 0.7 0.9 – 1.4 – 1.1 1.3 1.3
Intensity Change (Log2) – – – 0.02 0.07 -0.24 -0.3 -0.28 -0.34 -0.51 -0.47 -0.44 0.3 0.31 0.38 – 0.32 – 0.37 0.44 0.49


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.0571 0.5807 0.2156 0.268 -0.0842 0.4257 1.9446 2.4654 2.0405 1.9688 1.8262 2.2442 0.8331 1.2525 1.8902 0.8816 1.4279 1.4829
Actin 0.0241 0.0043 0.0035 0.0027 0.0061 0.01 0.0266 0.029 0.0401 0.0369 0.0176 0.0204 0.0056 0.0015 0.0458 0.011 0.0285 0.0052
Bud 0.0003 0.0011 0.0006 0.0005 0.0014 0.0005 0.0015 0.0034 0.0052 0.0029 0.0021 0.0014 0.0001 0.0007 0.0009 0.0003 0.0015 0.0012
Bud Neck 0.0008 0.0007 0.0047 0.0008 0.0013 0.0015 0.0095 0.0016 0.0059 0.0032 0.001 0.0126 0.0002 0.0011 0.0096 0.0012 0.0014 0.0038
Bud Periphery 0.0011 0.0018 0.0015 0.0014 0.0059 0.0009 0.0045 0.0063 0.0107 0.0052 0.0052 0.0039 0.0002 0.0007 0.0017 0.0005 0.0037 0.0025
Bud Site 0.0015 0.0067 0.0039 0.0005 0.0018 0.0011 0.0115 0.0381 0.0202 0.0046 0.0029 0.0022 0.0005 0.026 0.0094 0.0008 0.0036 0.0018
Cell Periphery 0.0008 0.0012 0.0015 0.0002 0.0007 0.0003 0.0024 0.0024 0.0036 0.0009 0.0009 0.0011 0.0002 0.0028 0.0016 0.0002 0.0012 0.0014
Cytoplasm 0.0677 0.1615 0.13 0.0853 0.1955 0.1438 0.1034 0.1863 0.1818 0.1554 0.1779 0.1668 0.028 0.2644 0.1564 0.1185 0.1438 0.1195
Cytoplasmic Foci 0.1687 0.1674 0.1447 0.1418 0.1875 0.1309 0.2014 0.1566 0.178 0.1798 0.1636 0.1902 0.372 0.2885 0.1063 0.2611 0.1295 0.1521
Eisosomes 0.0007 0.0004 0.0009 0.0003 0.0003 0.0002 0.0008 0.0008 0.0015 0.0009 0.001 0.0004 0.0002 0.0003 0.0033 0.0006 0.0006 0.0007
Endoplasmic Reticulum 0.0067 0.0041 0.0059 0.0019 0.0037 0.0054 0.0068 0.0029 0.0052 0.0033 0.0061 0.0033 0.0023 0.0061 0.0048 0.0027 0.0037 0.0041
Endosome 0.0643 0.0903 0.0768 0.1401 0.1577 0.0833 0.0994 0.0963 0.0683 0.1446 0.0939 0.074 0.0781 0.0773 0.0307 0.1201 0.0308 0.055
Golgi 0.0191 0.0229 0.0144 0.0248 0.0332 0.0158 0.0424 0.0389 0.0289 0.0449 0.0291 0.0308 0.0183 0.0222 0.0165 0.0184 0.0219 0.0252
Lipid Particles 0.0492 0.0145 0.021 0.0125 0.0061 0.0072 0.0304 0.006 0.0103 0.0196 0.0136 0.0118 0.0689 0.0089 0.0075 0.0207 0.0114 0.0281
Mitochondria 0.0247 0.0335 0.0498 0.0719 0.039 0.0366 0.0512 0.0469 0.104 0.1077 0.0797 0.0689 0.0244 0.012 0.0485 0.0182 0.0588 0.0942
None 0.5285 0.4368 0.4892 0.4606 0.3369 0.5281 0.3123 0.353 0.2777 0.2145 0.366 0.3329 0.2222 0.2657 0.5036 0.3212 0.5392 0.4289
Nuclear Periphery 0.0032 0.0011 0.0021 0.0007 0.0008 0.0009 0.0116 0.0006 0.0019 0.0013 0.0009 0.0011 0.0004 0.0006 0.0012 0.0008 0.0005 0.0008
Nucleolus 0.0011 0.0009 0.0008 0.0006 0.0006 0.0005 0.0016 0.0005 0.0009 0.0023 0.0004 0.0004 0.0002 0.0008 0.0005 0.0003 0.0002 0.0004
Nucleus 0.0012 0.0014 0.0014 0.0009 0.0019 0.0016 0.006 0.0008 0.002 0.0014 0.0007 0.0008 0.0002 0.0012 0.0008 0.0006 0.0005 0.0007
Peroxisomes 0.0277 0.0399 0.0359 0.0462 0.0117 0.024 0.061 0.0236 0.0442 0.0563 0.0289 0.0702 0.1752 0.0045 0.0454 0.0942 0.0132 0.0673
Punctate Nuclear 0.0028 0.0017 0.0047 0.001 0.0014 0.0024 0.006 0.001 0.003 0.0025 0.0008 0.0025 0.0008 0.0065 0.0011 0.0026 0.001 0.0018
Vacuole 0.0049 0.0061 0.0053 0.0036 0.0049 0.0038 0.0076 0.0037 0.0052 0.0082 0.0057 0.003 0.0015 0.0073 0.0035 0.0051 0.0034 0.0034
Vacuole Periphery 0.0011 0.0017 0.0015 0.0015 0.0016 0.0014 0.0021 0.0014 0.0016 0.0037 0.0021 0.0011 0.0005 0.0009 0.0007 0.001 0.0013 0.0019

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 10.9325 4.8865 6.216 9.1905 8.6059 7.8046 9.5286 8.3367 8.952 11.5415
Translational Efficiency 0.4161 0.6628 0.6554 0.448 0.4682 0.6361 0.4105 0.4611 0.4202 0.2739

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
123 1282 219 1293 1307 1650 2392 1717 1430 2932 2611 3010

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 837.93 763.92 911.37 841.55 627.23 764.18 823.49 801.20 645.35 764.07 830.86 818.53
Standard Deviation 102.76 105.94 92.05 119.09 69.51 94.89 91.20 93.38 93.89 99.87 94.46 107.08
Intensity Change Log 2 — -0.133408 0.121207 0.006219 — 0.284918 0.392757 0.353168 — 0.060681 0.243761 0.165055

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.003895 0.002466 0.006475 0.011876 0.001209 0.002169 0.002799 0.003941 0.001440 0.002299 0.003107 0.007350
Bud Neck 0.022596 0.022391 0.013586 0.028108 0.046203 0.045747 0.046910 0.031201 0.044173 0.035535 0.044115 0.029872
Bud Site 0.039837 0.024537 0.032769 0.085051 0.013070 0.026430 0.034011 0.036094 0.015372 0.025603 0.033907 0.057124
Cell Periphery 0.002481 0.000332 0.000438 0.000268 0.000366 0.000382 0.000133 0.000253 0.000548 0.000360 0.000158 0.000259
Cytoplasm 0.168856* 0.037408 0.207784* 0.242490* 0.071355 0.064456 0.293276* 0.263567* 0.079741 0.052629 0.286105* 0.254513*
Cytoplasmic Foci 0.205693* 0.205047* 0.147383 0.142342 0.277901* 0.250483* 0.137506 0.185658* 0.271690* 0.230617* 0.138334 0.167051
Eisosomes 0.000337 0.000255 0.000219 0.000202 0.000501 0.000297 0.000114 0.000207 0.000487 0.000279 0.000123 0.000205
Endoplasmic Reticulum 0.003152 0.001009 0.016982 0.003710 0.000641 0.000401 0.002750 0.002245 0.000857 0.000667 0.003944 0.002874
Endosome 0.072993 0.104789 0.060453 0.026198 0.073820 0.110118 0.025521 0.037758 0.073749 0.107788 0.028451 0.032792
Golgi 0.017501 0.125341* 0.005109 0.010808 0.070882 0.096161 0.005131 0.012087 0.066291 0.108920 0.005130 0.011538
Lipid Particles 0.005533 0.016118 0.018700 0.007962 0.027213 0.012271 0.007063 0.013619 0.025348 0.013953 0.008039 0.011189
Mitochondria 0.006882 0.274983* 0.002662 0.010706 0.281773* 0.214583* 0.020802 0.022482 0.258129* 0.240993* 0.019280 0.017424
Mitotic Spindle 0.008900 0.016263 0.040665 0.054699 0.003456 0.010591 0.059712 0.036187 0.003924 0.013071 0.058115 0.044139
None 0.000953 0.001556 0.001876 0.001444 0.001530 0.000517 0.001793 0.002378 0.001481 0.000972 0.001800 0.001977
Nuclear Periphery 0.000805 0.000264 0.002686 0.001369 0.000278 0.000115 0.002037 0.001199 0.000324 0.000181 0.002091 0.001272
Nuclear Periphery Foci 0.001066 0.000535 0.011527 0.002429 0.000236 0.000164 0.001067 0.001161 0.000307 0.000326 0.001944 0.001706
Nucleolus 0.001591 0.001786 0.003189 0.001713 0.001778 0.000885 0.004345 0.002969 0.001762 0.001279 0.004248 0.002429
Nucleus 0.140218* 0.007244 0.193387* 0.094669 0.014489 0.011585 0.171708* 0.124016 0.025304 0.009687 0.173526* 0.111410
Peroxisomes 0.075318 0.116965* 0.045843 0.082382 0.091095 0.106087* 0.064224 0.069913 0.089738 0.110844* 0.062682 0.075269
Vacuole 0.213969* 0.029445 0.185781 0.187902 0.016343 0.039504 0.117158 0.149968 0.033342 0.035106 0.122914 0.166264
Vacuole Periphery 0.007423 0.011265 0.002486 0.003672 0.005860 0.007050 0.001940 0.003095 0.005994 0.008893 0.001985 0.003343

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.10 -2.23 -3.47 -5.67 -1.52 -2.29 -4.26 -2.81 -1.45 -0.91 -2.41 -4.73 -6.51 -5.10 -4.21
Bud Neck -0.21 1.82 -0.81 -1.02 -4.58 0.09 -0.05 5.06 5.57 7.08 2.64 0.14 5.64 4.12 7.99
Bud Site 1.44 0.48 -3.20 -8.91 -5.91 -6.36 -8.18 -7.31 -1.82 -0.56 -5.81 -7.43 -12.20 -7.88 -5.68
Cell Periphery 1.10 1.03 1.18 3.82 5.93 -0.22 7.05 3.84 2.30 -7.43 1.04 2.67 2.13 3.45 -6.32
Cytoplasm 6.61 -1.75 -4.13 -26.89 -2.70 1.27 -31.66 -25.98 -28.37 2.24 5.74 -30.50 -28.48 -38.94 3.25
Cytoplasmic Foci -1.00 3.11 4.54 13.42 1.65 3.56 21.49 14.55 12.18 -7.02 5.87 21.50 18.97 17.58 -3.63
Eisosomes 0.78 1.13 2.10 3.45 3.40 4.24 8.49 6.71 6.11 -9.35 4.68 8.56 7.09 6.69 -9.04
Endoplasmic Reticulum 3.98 -7.67 -0.89 -10.71 7.73 1.90 -13.17 -7.20 -9.15 2.81 1.51 -14.04 -11.33 -13.91 4.57
Endosome -4.09 0.93 4.54 19.56 5.63 -7.25 14.01 10.83 18.99 -4.23 -8.27 13.73 13.40 27.02 -0.70
Golgi -14.28 1.55 1.14 24.29 -1.78 -5.13 19.77 17.57 23.15 -4.62 -9.99 19.57 17.92 33.42 -4.77
Lipid Particles -5.26 -6.10 -0.99 4.81 5.68 7.13 10.71 7.43 -0.02 -5.34 5.90 10.00 8.74 3.51 -2.56
Mitochondria -30.25* 1.43 -0.61 30.84* -4.00 6.17 30.12 29.79* 29.18* 0.09 1.77 29.20 29.58 42.19* 2.33
Mitotic Spindle -0.46 -2.31 -5.02 -7.07 -1.04 -4.82 -16.78 -9.73 -7.01 5.68 -6.79 -16.76 -13.68 -9.93 4.53
None -1.15 -2.25 -1.64 0.26 0.96 2.12 -0.31 -0.95 -4.03 -1.08 1.05 -0.48 -0.65 -2.48 -0.43
Nuclear Periphery 3.61 -5.16 -3.52 -9.76 3.15 2.13 -14.61 -9.76 -13.15 5.26 2.01 -15.36 -12.03 -16.08 5.66
Nuclear Periphery Foci 2.89 -7.71 -3.16 -6.23 6.88 1.16 -7.38 -7.59 -10.04 -0.34 0.29 -10.05 -9.48 -9.91 1.76
Nucleolus -0.23 -2.01 -0.02 0.29 2.76 2.19 -4.58 -1.82 -6.43 3.75 1.22 -4.76 -1.10 -3.77 5.22
Nucleus 6.79 -1.60 2.17 -21.81 5.91 1.48 -35.43 -25.69 -26.55 7.64 6.31 -31.37 -23.61 -34.27 11.36
Peroxisomes -3.78 1.90 0.62 8.98 -2.49 -2.53 7.32 6.30 8.81 -0.56 -4.34 7.55 6.69 12.57 -1.04
Vacuole 10.02 1.16 -1.37 -30.72 -4.12 -9.26 -33.19 -31.55 -25.72 -7.84 0.10 -25.16 -35.82 -39.46 -14.27
Vacuole Periphery -1.60 1.19 1.13 8.31 -0.31 -2.00 9.17 6.17 8.65 -2.19 -4.41 8.37 6.28 11.64 -2.41
Standard name
Human Ortholog
Description Mitochondrial inner membrane Mg(2+) channel; required for maintenance of intramitochondrial Mg(2+) concentrations at the correct level to support splicing of group II introns; similar to bacterial CorA
Localization
Cell Percentages mitochondrion (73%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Mrs2

Mrs2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mrs2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available