Standard name
Human Ortholog
Description Tubulin folding factor D involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Cytoplasm 0.97 0.98 0.99 0.98 0.98 0.96 0.94 0.89 0.91 0.86 0.77 0.82 1.0 0.98 0.99 0.94 0.93 0.97 0.94 0.9 0.84 0.91 0.9 0.88
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.13 0.16 0.22 0.31 0.29 0.46 0.38 0 0 0 0 0 0 0 0.05 0.06 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 3 3 3 1
Bud 0 2 3 0 2 5 5 14 5 5 11 7 1 0 4 0 0 0 0 0 4 0 3 3
Bud Neck 0 0 6 2 2 7 8 9 1 1 3 3 0 0 0 0 0 0 0 0 0 0 1 0
Bud Site 0 0 0 0 0 0 3 1 0 0 2 1 0 0 0 0 0 0
Cell Periphery 3 2 2 4 3 5 1 1 2 4 3 3 1 0 1 9 6 10 0 0 6 0 0 0
Cytoplasm 177 259 441 384 437 536 602 594 401 375 308 314 210 305 320 234 161 195 171 239 335 184 228 247
Endoplasmic Reticulum 2 0 1 3 1 0 0 1 0 0 0 0 1 5 0 11 7 12 0 1 1 1 1 4
Endosome 0 0 0 0 0 0 0 1 0 0 0 0 0 1 1 2 3 0 0 1 2 0 2 1
Golgi 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 4 2 2 1 0 0 0 0 0
Mitochondria 0 10 3 14 20 72 105 149 138 126 183 146 1 1 8 7 1 4 3 13 24 3 6 9
Nucleus 1 1 0 1 1 3 2 2 3 3 2 1 0 1 1 0 0 1 0 0 0 0 0 0
Nuclear Periphery 1 1 1 1 0 0 0 1 0 0 0 1 0 0 0 1 0 1 0 0 0 0 0 0
Nucleolus 1 0 0 0 0 1 0 2 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
SpindlePole 0 0 1 0 0 2 6 5 3 6 3 6 0 0 0 1 2 0 0 0 3 1 0 0
Vac/Vac Membrane 1 0 0 1 0 1 1 1 2 1 1 2 0 1 0 5 6 2 0 3 2 1 1 5
Unique Cell Count 182 265 446 390 448 557 640 666 442 434 402 381 211 311 323 248 173 202 183 265 399 204 255 282
Labelled Cell Count 187 275 458 410 466 632 733 781 555 521 517 484 214 314 335 274 188 227 183 265 399 204 255 282


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.8 3.6 3.2 3.1 3.4 3.0 2.9 3.1 2.6 2.7 2.6 2.5 4.2 4.4 4.0 6.1 7.1 6.6 4.6 4.6 5.1
Std Deviation (1e-4) 0.5 0.5 0.7 0.9 1.3 1.1 1.2 1.7 1.1 2.3 1.0 1.2 1.3 1.2 1.1 1.3 2.5 2.2 1.5 1.6 1.6
Intensity Change (Log2) -0.05 0.11 -0.07 -0.12 -0.04 -0.32 -0.22 -0.3 -0.35 0.39 0.46 0.34 0.94 1.15 1.05 0.53 0.53 0.68


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration
Actin 0.039 0.0004 0.0134 0.0495 0.0438 0.0117 0.0109 0.0002 0.0042 0.0027 0.01 0.0007
Bud 0.0017 0.0008 0.0009 0.0081 0.0006 0.0002 0.0052 0.0001 0.008 0.0001 0.0143 0.0017
Bud Neck 0.0027 0.0003 0.0032 0.001 0.0041 0.0012 0.0002 0.0001 0.0004 0.0001 0.0003 0.001
Bud Periphery 0.005 0.0007 0.0012 0.0089 0.0018 0.0003 0.0065 0.0001 0.0135 0.0001 0.0157 0.0007
Bud Site 0.0079 0.0124 0.0109 0.0045 0.0012 0.0012 0.0034 0.0012 0.0103 0.0001 0.0008 0.0001
Cell Periphery 0.001 0.0006 0.0006 0.0006 0.0004 0.0001 0.0004 0.0001 0.0009 0.0001 0.0003 0.0001
Cytoplasm 0.1771 0.3278 0.2384 0.2671 0.2663 0.3566 0.0969 0.2531 0.1755 0.1711 0.1759 0.2112
Cytoplasmic Foci 0.0128 0.0073 0.0151 0.0104 0.0107 0.0194 0.0077 0.0045 0.0057 0.0078 0.02 0.014
Eisosomes 0.0012 0.0001 0.0004 0.0027 0.0013 0.0002 0.0002 0.0001 0.0002 0.0003 0.0001 0
Endoplasmic Reticulum 0.005 0.0025 0.003 0.0021 0.0038 0.0017 0.001 0.0012 0.0015 0.0003 0.0008 0.0005
Endosome 0.0101 0.0025 0.009 0.0061 0.0059 0.0031 0.0013 0.0014 0.0025 0.0007 0.0011 0.0013
Golgi 0.0039 0.0003 0.0039 0.007 0.0072 0.0016 0.0007 0.0001 0.0007 0.0002 0.0003 0.0002
Lipid Particles 0.004 0.0017 0.0077 0.0027 0.0138 0.0026 0.0038 0.0002 0.001 0.0003 0.0005 0.0002
Mitochondria 0.0031 0.0005 0.0102 0.0972 0.0348 0.0017 0.0009 0.0002 0.0014 0.0001 0.0016 0.0008
None 0.7013 0.6364 0.6593 0.5134 0.5908 0.5887 0.8484 0.7337 0.7604 0.8144 0.738 0.7603
Nuclear Periphery 0.0037 0.0005 0.0039 0.0012 0.0013 0.0005 0.0004 0.0005 0.0018 0.0001 0.0012 0.0003
Nucleolus 0.0005 0.0002 0.0009 0.0007 0.0004 0.0003 0.0004 0.0001 0.0005 0.0001 0.0013 0.0003
Nucleus 0.0025 0.0019 0.002 0.0032 0.0011 0.0008 0.0008 0.0013 0.0024 0.0003 0.0035 0.0014
Peroxisomes 0.0024 0.0002 0.0101 0.0041 0.0071 0.0038 0.0089 0.0001 0.0032 0.0005 0.0073 0.0015
Punctate Nuclear 0.0088 0.0011 0.0036 0.0053 0.0018 0.0038 0.0011 0.0008 0.0041 0.0003 0.0055 0.0029
Vacuole 0.0046 0.0017 0.0017 0.0025 0.0011 0.0004 0.0008 0.0007 0.0014 0.0003 0.0011 0.0006
Vacuole Periphery 0.0016 0.0001 0.0007 0.0014 0.0004 0.0001 0.0001 0.0001 0.0004 0 0.0004 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 3.986 2.5915 2.1634 4.981 3.7586 2.8973 4.9948 4.4212 5.5388 4.7585
Translational Efficiency

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1480 1014 2115 997 1806 1277 158 568 3286 2291 2273 1565

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 606.05 692.73 911.79 797.75 657.84 715.01 902.26 794.60 634.51 705.15 911.13 796.61
Standard Deviation 71.77 93.17 106.04 98.81 72.85 91.23 83.92 103.40 76.82 92.76 104.69 100.51
Intensity Change Log 2 0.192856 0.589265 0.396500 0.120227 0.455806 0.272492 0.155510 0.521343 0.333287

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000106 0.001167 0.000081 0.000627 0.000166 0.000529 0.000277 0.000985 0.000139 0.000811 0.000095 0.000757
Bud Neck 0.016322 0.037793 0.005047 0.011844 0.017987 0.037265 0.006398 0.007658 0.017237 0.037499 0.005141 0.010325
Bud Site 0.005012 0.017431 0.013213 0.051227 0.006067 0.022387 0.003444 0.024860 0.005592 0.020193 0.012534 0.041657
Cell Periphery 0.000157 0.000157 0.000077 0.000165 0.000103 0.000170 0.000289 0.000123 0.000127 0.000164 0.000092 0.000150
Cytoplasm 0.543123* 0.408663* 0.546694* 0.466524* 0.483094* 0.413383* 0.273668* 0.479367* 0.510131* 0.411294* 0.527715* 0.471185*
Cytoplasmic Foci 0.189078* 0.277497* 0.006558 0.022944 0.222348* 0.219955* 0.047693 0.050310 0.207363* 0.245423* 0.009418 0.032876
Eisosomes 0.000167 0.000151 0.000014 0.000048 0.000139 0.000155 0.000171 0.000062 0.000152 0.000153 0.000025 0.000053
Endoplasmic Reticulum 0.000516 0.000440 0.000927 0.001788 0.000925 0.001017 0.004040 0.001664 0.000741 0.000762 0.001143 0.001743
Endosome 0.013877 0.037652 0.001254 0.008113 0.009755 0.028781 0.007748 0.014370 0.011612 0.032707 0.001705 0.010384
Golgi 0.002221 0.006297 0.000048 0.003537 0.002531 0.006324 0.000136 0.010481 0.002392 0.006312 0.000054 0.006057
Lipid Particles 0.008549 0.007109 0.000371 0.003362 0.009122 0.009195 0.006070 0.001831 0.008864 0.008272 0.000767 0.002806
Mitochondria 0.003225 0.012973 0.000412 0.005713 0.007616 0.011599 0.000728 0.008373 0.005638 0.012207 0.000434 0.006679
Mitotic Spindle 0.001550 0.003748 0.002196 0.059131 0.001934 0.014993 0.000486 0.015693 0.001761 0.010016 0.002078 0.043365
None 0.008728 0.004105 0.006062 0.003914 0.002412 0.003400 0.003293 0.006905 0.005256 0.003712 0.005870 0.005000
Nuclear Periphery 0.000432 0.000392 0.000726 0.001228 0.000463 0.000490 0.001902 0.001162 0.000449 0.000447 0.000808 0.001204
Nuclear Periphery Foci 0.000308 0.000382 0.000617 0.001876 0.000227 0.000260 0.002521 0.001455 0.000263 0.000314 0.000749 0.001723
Nucleolus 0.002409 0.003769 0.000180 0.000592 0.000974 0.002024 0.001704 0.000535 0.001620 0.002796 0.000286 0.000571
Nucleus 0.148365* 0.075002 0.382574* 0.214076* 0.179805* 0.121604 0.502600* 0.151779* 0.165645* 0.100978 0.390917* 0.191466*
Peroxisomes 0.003364 0.021959 0.000111 0.000888 0.007168 0.012580 0.000921 0.002926 0.005454 0.016731 0.000167 0.001628
Vacuole 0.051569 0.080254 0.032685 0.141609 0.046269 0.091389 0.135455 0.213977* 0.048656 0.086460 0.039829 0.167874
Vacuole Periphery 0.000923 0.003060 0.000154 0.000793 0.000894 0.002503 0.000455 0.005484 0.000907 0.002750 0.000175 0.002495

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.34 -0.04 -4.77 0.87 -5.06 -1.47 -3.72 -1.62 0.02 -0.76 -1.79 0.75 -3.75 0.82 -3.97
Bud Neck -6.92 9.07 6.16 10.20 -2.29 -6.72 4.43 9.24 11.64 -0.09 -9.60 12.80 10.45 15.19 -1.85
Bud Site -8.24 -3.55 -5.34 -1.09 -3.08 -6.30 2.01 -2.68 2.13 -3.57 -9.16 -3.10 -5.84 0.67 -3.22
Cell Periphery 0.11 8.13 3.46 1.68 -4.77 -6.03 -7.98 0.61 6.35 8.17 -2.95 5.42 2.37 4.41 -2.86
Cytoplasm 12.37 4.16 17.15 3.89 14.41 7.03 11.79 7.16 2.00 -5.79 13.50 2.71 16.91 4.61 13.58
Cytoplasmic Foci -10.49 40.21 37.23 37.32 -8.55 0.33 27.44 33.78 30.07 1.17 -6.62 57.28 51.14 48.62 -10.65
Eisosomes 1.48 18.47 12.15 9.85 -17.51 -1.54 -2.41 9.09 10.36 7.78 -0.10 22.89 15.25 14.59 -15.91
Endoplasmic Reticulum 1.10 -9.68 -15.07 -15.88 -9.48 -0.18 -6.09 -5.74 -2.27 4.60 -0.07 -8.73 -14.45 -6.84 -6.52
Endosome -8.70 10.03 7.59 13.74 -4.27 -9.71 1.35 1.85 10.21 0.60 -12.92 13.28 7.72 17.72 -5.87
Golgi -4.84 4.42 1.70 6.02 -1.53 -3.80 9.22 -0.70 1.18 -2.03 -5.83 8.87 0.51 4.38 -2.54
Lipid Particles 1.59 10.29 6.85 6.62 -2.96 -0.08 3.16 9.87 8.79 7.91 0.83 14.83 10.86 10.07 -3.12
Mitochondria -5.53 5.18 1.48 6.06 -2.02 -2.30 6.25 2.23 3.98 -1.59 -5.27 7.92 3.27 7.60 -2.56
Mitotic Spindle -1.96 -1.29 -7.16 -6.52 -6.92 -4.87 1.99 -3.24 0.54 -3.76 -5.24 -1.10 -7.82 -4.45 -7.54
None 2.57 2.14 2.96 -0.19 3.19 -1.51 -0.94 -2.34 -1.39 -1.65 1.67 -0.30 0.61 -1.29 1.35
Nuclear Periphery 0.36 -7.56 -15.15 -14.69 -12.54 -0.38 -11.09 -11.20 -11.28 3.98 0.00 -11.77 -18.25 -18.05 -12.80
Nuclear Periphery Foci -1.17 -7.75 -2.89 -2.75 -1.93 -1.15 -6.40 -7.51 -7.28 3.77 -1.55 -10.64 -4.03 -3.89 -1.96
Nucleolus -1.40 5.20 3.95 3.22 -3.68 -3.74 -0.78 2.83 6.05 2.69 -2.39 6.69 4.69 4.83 -3.42
Nucleus 13.27 -38.66 -14.16 -22.73 15.19 9.47 -12.43 0.68 -5.57 12.13 15.19 -40.33 -10.53 -20.26 22.75
Peroxisomes -9.22 8.90 7.32 10.85 -2.71 -4.22 8.71 6.61 9.74 -1.14 -9.84 12.35 9.78 14.90 -3.21
Vacuole -7.62 0.51 -24.77 -20.36 -24.85 -10.68 -8.85 -23.60 -19.32 -6.66 -13.08 -3.98 -34.13 -27.56 -31.18
Vacuole Periphery -6.18 8.64 6.18 8.25 -3.00 -5.17 3.73 -1.37 0.45 -1.96 -7.95 12.72 -0.11 4.61 -2.51
Standard name
Human Ortholog
Description Tubulin folding factor D involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl
Localization
Cell Percentages cytoplasm (22%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Cin1

Cin1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cin1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available