Standard name | |
---|---|
Human Ortholog | |
Description | Subunit of TFIIH complex; facilities dissociation of the Ssl2p helices from TFIIH; expression levels regulated by Arg5,6p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 5 |
Bud | 1 | – | 0 | 1 | 0 | 0 | 7 | 6 | 4 | 7 | 12 | 7 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 1 | 10 | 17 |
Bud Neck | 1 | – | 0 | 1 | 5 | 2 | 5 | 9 | 1 | 4 | 2 | 2 | 0 | 2 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 4 | 7 |
Bud Site | 0 | – | 0 | 0 | 0 | 5 | 5 | 5 | 8 | 2 | 6 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | – | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 184 | – | 28 | 116 | 159 | 100 | 131 | 163 | 108 | 107 | 63 | 80 | 129 | 145 | 160 | 64 | 37 | 48 | 155 | 26 | 32 | 51 | 188 | 228 |
Endoplasmic Reticulum | 2 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 10 | 12 | 6 | 0 | 0 | 0 | 0 | 0 | 1 |
Endosome | 0 | – | 0 | 1 | 2 | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 6 | 0 | 1 | 11 | 3 | 4 | 3 | 0 | 1 | 0 | 4 | 3 |
Golgi | 0 | – | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 3 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 36 | – | 5 | 8 | 13 | 64 | 176 | 148 | 199 | 169 | 212 | 170 | 5 | 1 | 0 | 14 | 3 | 3 | 10 | 2 | 2 | 2 | 5 | 8 |
Nucleus | 16 | – | 3 | 41 | 83 | 115 | 221 | 221 | 155 | 231 | 115 | 193 | 55 | 80 | 98 | 116 | 65 | 103 | 29 | 9 | 7 | 18 | 57 | 76 |
Nuclear Periphery | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Nucleolus | 0 | – | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 1 | 2 | 1 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 |
Peroxisomes | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | – | 0 | 3 | 0 | 1 | 3 | 5 | 2 | 2 | 2 | 2 | 1 | 0 | 0 | 1 | 0 | 0 | 6 | 0 | 1 | 2 | 15 | 24 |
Vac/Vac Membrane | 16 | – | 7 | 20 | 28 | 31 | 22 | 18 | 11 | 16 | 14 | 18 | 29 | 34 | 42 | 163 | 73 | 101 | 20 | 9 | 5 | 10 | 31 | 42 |
Unique Cell Count | 228 | 39 | 180 | 281 | 272 | 454 | 479 | 382 | 438 | 302 | 373 | 208 | 247 | 284 | 325 | 168 | 237 | 235 | 52 | 60 | 94 | 327 | 433 | |
Labelled Cell Count | 257 | 43 | 193 | 290 | 321 | 571 | 578 | 493 | 540 | 428 | 479 | 226 | 263 | 303 | 383 | 196 | 268 | 235 | 52 | 60 | 94 | 327 | 433 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.3 | – | 4.8 | 4.8 | 5.3 | 4.2 | 4.0 | 4.5 | 3.9 | 4.2 | 3.8 | 4.2 | 5.3 | 5.3 | 5.4 | 9.1 | 9.2 | 9.5 | 4.4 | 5.2 | 5.5 |
Std Deviation (1e-4) | 0.7 | – | 1.5 | 1.2 | 1.9 | 1.1 | 1.3 | 1.4 | 1.0 | 1.2 | 1.5 | 1.4 | 1.8 | 1.4 | 1.3 | 2.7 | 2.7 | 2.6 | 0.9 | 1.3 | 1.3 |
Intensity Change (Log2) | – | – | – | -0.0 | 0.12 | -0.21 | -0.27 | -0.1 | -0.31 | -0.2 | -0.35 | -0.19 | 0.13 | 0.14 | 0.15 | 0.92 | 0.93 | 0.98 | -0.12 | 0.1 | 0.18 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -0.9 | -1.8 | -4.2 | -5.5 | -4.7 | -5.5 | -6.3 | -6.8 | -6.8 | -1.2 | -1.6 | -1.8 | -7.1 | -6.0 | -6.7 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 2.1 | 2.9 | 4.2 | 4.9 | 4.7 | 4.0 | 5.4 | 3.8 | 5.2 | 2.5 | 3.2 | 3.4 | 3.5 | 3.7 | 4.3 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -0.7 | -0.7 | -0.5 | 3.8 | 2.9 | 2.9 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.7228 | 1.0471 | 1.1231 | 1.0092 | 1.1195 | 0.8001 | -0.028 | 0.4581 | 0.3479 | -0.2993 | -0.1961 | 0.432 | -0.9311 | -0.7648 | -0.541 | -1.0514 | -1.6387 | -0.5053 |
Actin | 0.0074 | 0.0003 | 0.0121 | 0.0006 | 0.0069 | 0.0011 | 0.021 | 0.0003 | 0.0157 | 0.0169 | 0.0004 | 0.0164 | 0.0528 | 0.0002 | 0.0249 | 0.0001 | 0.0003 | 0.0015 |
Bud | 0.0011 | 0.0005 | 0.0009 | 0.0002 | 0.0006 | 0.0003 | 0.0005 | 0.0006 | 0.0002 | 0.0004 | 0 | 0.0002 | 0.0005 | 0.0001 | 0.0005 | 0.0001 | 0 | 0.0001 |
Bud Neck | 0.0008 | 0.0008 | 0.0022 | 0.0002 | 0.002 | 0.0043 | 0.0046 | 0.0004 | 0.0014 | 0.0004 | 0.0003 | 0.0024 | 0.0009 | 0.0002 | 0.0007 | 0.0002 | 0.0006 | 0.0025 |
Bud Periphery | 0.0024 | 0.0002 | 0.0013 | 0.0001 | 0.0005 | 0.0006 | 0.0007 | 0.0004 | 0.0003 | 0.0007 | 0 | 0.0003 | 0.0005 | 0 | 0.0006 | 0 | 0 | 0.0001 |
Bud Site | 0.0023 | 0.0114 | 0.0144 | 0.0004 | 0.0015 | 0.0004 | 0.0032 | 0.001 | 0.0021 | 0.0014 | 0.0001 | 0.0003 | 0.0069 | 0.0008 | 0.008 | 0.0001 | 0.0001 | 0.0001 |
Cell Periphery | 0.0002 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0002 | 0.0001 | 0.0006 | 0 | 0 | 0 |
Cytoplasm | 0.1027 | 0.1487 | 0.0966 | 0.0458 | 0.0995 | 0.0923 | 0.102 | 0.1214 | 0.0951 | 0.0958 | 0.1205 | 0.0789 | 0.2283 | 0.1287 | 0.2517 | 0.1058 | 0.3368 | 0.2002 |
Cytoplasmic Foci | 0.0222 | 0.0036 | 0.0139 | 0.0102 | 0.0238 | 0.0051 | 0.0176 | 0.002 | 0.0122 | 0.0026 | 0.0041 | 0.0044 | 0.0125 | 0.0034 | 0.0201 | 0.0014 | 0.0058 | 0.0021 |
Eisosomes | 0.0004 | 0 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0005 | 0 | 0.0003 | 0.0003 | 0 | 0.0002 | 0.0005 | 0 | 0.0003 | 0.0002 | 0 | 0 |
Endoplasmic Reticulum | 0.0036 | 0.0055 | 0.0026 | 0.0032 | 0.0012 | 0.0013 | 0.0038 | 0.0019 | 0.0022 | 0.0018 | 0.0014 | 0.0018 | 0.014 | 0.0027 | 0.0067 | 0.0008 | 0.0101 | 0.0016 |
Endosome | 0.0167 | 0.0018 | 0.0075 | 0.077 | 0.0236 | 0.0058 | 0.0117 | 0.0014 | 0.0077 | 0.0035 | 0.0014 | 0.0053 | 0.0143 | 0.0025 | 0.0171 | 0.0006 | 0.0076 | 0.0076 |
Golgi | 0.004 | 0.0001 | 0.0031 | 0.0072 | 0.019 | 0.0047 | 0.004 | 0.0001 | 0.0035 | 0.0022 | 0.0001 | 0.0028 | 0.0042 | 0.0001 | 0.0077 | 0 | 0.0001 | 0.0178 |
Lipid Particles | 0.0109 | 0.0003 | 0.003 | 0.0012 | 0.012 | 0.0044 | 0.0054 | 0.0001 | 0.0108 | 0.0043 | 0.0001 | 0.0043 | 0.0082 | 0.0005 | 0.016 | 0.0001 | 0.0002 | 0.0005 |
Mitochondria | 0.0165 | 0.0007 | 0.0208 | 0.01 | 0.0072 | 0.0103 | 0.0035 | 0.0007 | 0.0043 | 0.0098 | 0.0004 | 0.0014 | 0.0033 | 0.0002 | 0.0135 | 0.0004 | 0.0005 | 0.0134 |
None | 0.2292 | 0.1705 | 0.2681 | 0.3174 | 0.1888 | 0.2154 | 0.3057 | 0.2382 | 0.2718 | 0.2518 | 0.3455 | 0.1856 | 0.2279 | 0.3069 | 0.3227 | 0.4865 | 0.5046 | 0.3194 |
Nuclear Periphery | 0.0194 | 0.0133 | 0.0178 | 0.0071 | 0.0114 | 0.0072 | 0.0163 | 0.0085 | 0.0099 | 0.0146 | 0.0119 | 0.01 | 0.0196 | 0.0136 | 0.0135 | 0.0029 | 0.0246 | 0.0047 |
Nucleolus | 0.0049 | 0.0031 | 0.0028 | 0.0018 | 0.0125 | 0.008 | 0.0045 | 0.0045 | 0.0028 | 0.0024 | 0.0022 | 0.0046 | 0.0019 | 0.0012 | 0.002 | 0.0008 | 0.0007 | 0.0018 |
Nucleus | 0.5113 | 0.6187 | 0.4851 | 0.4821 | 0.504 | 0.6021 | 0.4564 | 0.5965 | 0.5208 | 0.5734 | 0.497 | 0.6404 | 0.3721 | 0.5289 | 0.2567 | 0.3975 | 0.0938 | 0.4209 |
Peroxisomes | 0.0085 | 0.0001 | 0.0067 | 0.0007 | 0.0266 | 0.0016 | 0.0076 | 0.0001 | 0.0133 | 0.0027 | 0.0002 | 0.0013 | 0.0085 | 0 | 0.0156 | 0 | 0.0001 | 0.0002 |
Punctate Nuclear | 0.0315 | 0.018 | 0.0373 | 0.0294 | 0.0572 | 0.0333 | 0.0262 | 0.0197 | 0.0237 | 0.0133 | 0.014 | 0.0378 | 0.0181 | 0.0056 | 0.0126 | 0.0015 | 0.0102 | 0.0044 |
Vacuole | 0.0028 | 0.0019 | 0.0024 | 0.0029 | 0.001 | 0.0011 | 0.0034 | 0.0018 | 0.0015 | 0.0011 | 0.0004 | 0.0011 | 0.004 | 0.004 | 0.0073 | 0.0007 | 0.0033 | 0.0006 |
Vacuole Periphery | 0.0012 | 0.0004 | 0.0012 | 0.0024 | 0.0006 | 0.0005 | 0.0011 | 0.0004 | 0.0004 | 0.0005 | 0.0001 | 0.0004 | 0.0008 | 0.0003 | 0.0012 | 0.0001 | 0.0006 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 5.8805 | 10.5137 | 2.837 | 7.5405 | 9.7038 | 10.686 | 7.9728 | 8.8054 | 8.3416 | 10.8003 |
Translational Efficiency | 2.0591 | 1.0875 | 3.1001 | 0.9843 | 1.1462 | 1.4669 | 1.9376 | 1.4161 | 1.816 | 1.7011 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1312 | 370 | 2042 | 930 | 1268 | 2032 | 909 | 921 | 2580 | 2402 | 2951 | 1851 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 672.91 | 765.86 | 833.41 | 824.08 | 655.82 | 725.25 | 939.62 | 889.51 | 664.51 | 731.51 | 866.13 | 856.64 |
Standard Deviation | 83.37 | 94.98 | 126.22 | 127.97 | 87.91 | 104.45 | 134.34 | 136.50 | 86.06 | 104.09 | 137.80 | 136.27 |
Intensity Change Log 2 | — | 0.186667 | 0.308613 | 0.292371 | — | 0.145178 | 0.518778 | 0.439711 | — | 0.166339 | 0.416169 | 0.366974 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000274 | 0.000533 | 0.000306 | 0.000963 | 0.000093 | 0.000211 | 0.000697 | 0.000741 | 0.000185 | 0.000261 | 0.000427 | 0.000853 |
Bud Neck | 0.009309 | 0.016459 | 0.015247 | 0.011424 | 0.006072 | 0.023011 | 0.004926 | 0.013492 | 0.007718 | 0.022002 | 0.012068 | 0.012453 |
Bud Site | 0.002713 | 0.009047 | 0.008912 | 0.011612 | 0.002857 | 0.011164 | 0.004738 | 0.017153 | 0.002784 | 0.010838 | 0.007626 | 0.014369 |
Cell Periphery | 0.000161 | 0.000231 | 0.000159 | 0.000204 | 0.000168 | 0.000164 | 0.000128 | 0.000185 | 0.000164 | 0.000174 | 0.000149 | 0.000194 |
Cytoplasm | 0.377562* | 0.269200* | 0.109902* | 0.155162* | 0.395533* | 0.359569* | 0.067842 | 0.144322* | 0.386394* | 0.345649* | 0.096946 | 0.149769* |
Cytoplasmic Foci | 0.178101 | 0.149235 | 0.001313 | 0.008129 | 0.104984 | 0.092738 | 0.000927 | 0.004752 | 0.142166 | 0.101440 | 0.001194 | 0.006449 |
Eisosomes | 0.000122 | 0.000117 | 0.000019 | 0.000047 | 0.000069 | 0.000068 | 0.000031 | 0.000036 | 0.000096 | 0.000076 | 0.000023 | 0.000041 |
Endoplasmic Reticulum | 0.000922 | 0.003131 | 0.003952 | 0.007876 | 0.001278 | 0.000899 | 0.008131 | 0.005581 | 0.001097 | 0.001243 | 0.005239 | 0.006734 |
Endosome | 0.006740 | 0.009525 | 0.000600 | 0.003002 | 0.004071 | 0.007965 | 0.000743 | 0.003223 | 0.005428 | 0.008205 | 0.000644 | 0.003112 |
Golgi | 0.001949 | 0.001513 | 0.000045 | 0.004563 | 0.000474 | 0.002660 | 0.000066 | 0.001071 | 0.001224 | 0.002483 | 0.000052 | 0.002825 |
Lipid Particles | 0.010047 | 0.010607 | 0.000361 | 0.001369 | 0.005526 | 0.004153 | 0.000606 | 0.000660 | 0.007825 | 0.005147 | 0.000437 | 0.001017 |
Mitochondria | 0.007118 | 0.007324 | 0.001789 | 0.004483 | 0.003932 | 0.007461 | 0.001046 | 0.002767 | 0.005552 | 0.007440 | 0.001560 | 0.003630 |
Mitotic Spindle | 0.001513 | 0.003416 | 0.004940 | 0.019109 | 0.001044 | 0.010686 | 0.004028 | 0.030424 | 0.001283 | 0.009566 | 0.004659 | 0.024739 |
None | 0.005850 | 0.004091 | 0.004725 | 0.008124 | 0.009325 | 0.005627 | 0.004681 | 0.008089 | 0.007558 | 0.005390 | 0.004711 | 0.008107 |
Nuclear Periphery | 0.000907 | 0.001013 | 0.001781 | 0.003280 | 0.000947 | 0.000919 | 0.001498 | 0.001963 | 0.000927 | 0.000934 | 0.001694 | 0.002625 |
Nuclear Periphery Foci | 0.000542 | 0.001420 | 0.000320 | 0.002476 | 0.000708 | 0.000434 | 0.000498 | 0.000475 | 0.000624 | 0.000586 | 0.000374 | 0.001480 |
Nucleolus | 0.002061 | 0.003157 | 0.000472 | 0.002346 | 0.002121 | 0.003503 | 0.000610 | 0.001966 | 0.002091 | 0.003450 | 0.000514 | 0.002157 |
Nucleus | 0.362080* | 0.457466* | 0.832377* | 0.713622* | 0.438422* | 0.435016* | 0.881946* | 0.726411* | 0.399600* | 0.438474* | 0.847646* | 0.719985* |
Peroxisomes | 0.008939 | 0.012794 | 0.000088 | 0.001301 | 0.002145 | 0.006551 | 0.000727 | 0.000759 | 0.005600 | 0.007513 | 0.000285 | 0.001031 |
Vacuole | 0.022451 | 0.038423 | 0.012504 | 0.039593 | 0.019507 | 0.025728 | 0.016024 | 0.035530 | 0.021004 | 0.027683 | 0.013588 | 0.037571 |
Vacuole Periphery | 0.000639 | 0.001298 | 0.000189 | 0.001315 | 0.000724 | 0.001473 | 0.000107 | 0.000400 | 0.000681 | 0.001446 | 0.000164 | 0.000860 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -1.98 | -0.72 | -3.45 | -2.00 | -4.20 | -4.55 | -11.84 | -8.35 | -6.82 | 1.61 | -1.22 | -5.41 | -6.84 | -7.55 | -3.58 |
Bud Neck | -2.57 | -4.10 | -1.82 | 1.44 | 2.02 | -9.81 | -1.64 | -6.00 | 4.05 | -4.34 | -9.38 | -5.13 | -5.31 | 5.06 | 0.06 |
Bud Site | -3.86 | -4.39 | -3.36 | 0.29 | -0.34 | -5.63 | -1.62 | -3.97 | 0.51 | -2.43 | -6.86 | -4.52 | -5.19 | 0.92 | -1.56 |
Cell Periphery | -2.74 | 0.51 | -0.24 | 2.57 | -0.70 | 0.38 | 1.13 | 0.02 | -0.38 | -1.17 | -0.75 | 1.07 | -0.15 | 0.62 | -1.21 |
Cytoplasm | 7.35 | 33.74 | 25.32 | 9.48 | -3.77 | 3.58 | 38.96 | 28.56 | 28.55 | -8.62 | 5.22 | 49.56 | 38.07 | 31.14 | -6.84 |
Cytoplasmic Foci | 2.57 | 31.02 | 30.01 | 14.24 | -4.68 | 2.28 | 23.60 | 22.99 | 26.66 | -5.51 | 8.30 | 38.22 | 37.12 | 29.64 | -6.21 |
Eisosomes | 0.19 | 14.02 | 8.54 | 6.99 | -12.19 | 0.22 | 5.34 | 3.13 | 3.00 | -2.76 | 4.00 | 16.16 | 8.96 | 5.60 | -12.91 |
Endoplasmic Reticulum | -5.35 | -17.89 | -16.38 | -8.80 | -8.16 | 2.07 | -16.37 | -14.52 | -16.15 | 3.58 | -1.56 | -22.81 | -21.89 | -21.47 | -5.07 |
Endosome | -2.14 | 11.36 | 7.61 | 5.25 | -4.88 | -4.52 | 6.43 | 3.40 | 7.18 | -2.21 | -3.84 | 13.06 | 7.80 | 8.92 | -5.01 |
Golgi | 1.04 | 5.86 | 0.12 | -0.27 | -1.56 | -4.31 | 5.34 | 0.32 | 4.18 | -1.66 | -2.70 | 6.88 | 0.19 | 1.88 | -1.83 |
Lipid Particles | -0.19 | 12.19 | 10.82 | 4.75 | -4.78 | 1.96 | 7.53 | 7.33 | 9.43 | -1.32 | 4.16 | 14.38 | 13.02 | 9.50 | -5.67 |
Mitochondria | -0.01 | 3.91 | 2.91 | 1.70 | -0.95 | -2.62 | 2.44 | 1.50 | 5.69 | -2.48 | -1.64 | 4.38 | 3.24 | 5.38 | -1.53 |
Mitotic Spindle | -0.95 | -3.09 | -5.56 | -4.60 | -4.28 | -5.49 | -2.78 | -6.47 | -3.65 | -4.89 | -5.51 | -4.26 | -8.52 | -4.77 | -6.54 |
None | 1.76 | 1.64 | -0.43 | -2.55 | -2.78 | 2.63 | 3.81 | 1.75 | -1.47 | -3.77 | 2.55 | 3.99 | 1.14 | -2.04 | -4.50 |
Nuclear Periphery | -0.71 | -9.79 | -6.84 | -6.33 | -3.82 | 0.03 | -8.66 | -12.88 | -13.32 | -7.06 | -0.35 | -13.33 | -11.77 | -11.66 | -6.53 |
Nuclear Periphery Foci | -3.78 | 0.59 | -2.98 | -1.00 | -3.11 | 1.61 | -1.33 | -1.45 | -3.40 | -0.17 | 0.12 | 0.12 | -3.31 | -3.36 | -3.32 |
Nucleolus | -1.85 | 8.04 | 0.55 | 1.81 | -2.23 | -2.23 | 3.14 | 0.77 | 3.17 | -2.75 | -2.91 | 6.71 | 0.92 | 3.29 | -3.48 |
Nucleus | -4.78 | -51.35 | -27.94 | -12.88 | 11.23 | 0.30 | -40.70 | -21.70 | -23.67 | 13.36 | -4.35 | -63.70 | -35.02 | -29.12 | 16.69 |
Peroxisomes | -1.66 | 8.88 | 7.84 | 5.90 | -1.71 | -7.32 | 3.45 | 6.60 | 11.38 | 0.99 | -2.44 | 9.95 | 9.20 | 12.08 | -1.07 |
Vacuole | -4.51 | 2.98 | -10.78 | -5.10 | -11.77 | -3.35 | -4.01 | -11.70 | -10.51 | -8.67 | -4.66 | -0.60 | -15.91 | -13.36 | -15.32 |
Vacuole Periphery | -2.02 | 7.18 | 0.11 | 1.65 | -1.64 | -2.05 | 3.60 | 2.66 | 3.66 | -2.86 | -2.60 | 5.86 | 1.53 | 3.24 | -2.05 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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