Standard name
Human Ortholog
Description Multicopy suppressor of HER2 involved in mitochondrial translation; mutant is defective in directing meiotic recombination events to homologous chromatids

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.11 0.1 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0.06 0 0.05 0.08 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0
Golgi 0 0 0.14 0.07 0.11 0 0 0 0 0 0 0 0.06 0.14 0.51 0.62 0.61 0 0.07 0 0 0.06 0.06
Mitochondria 0.93 0.91 0.81 0.92 0.82 0.88 0.83 0.69 0.92 0.9 0.89 0.94 0.92 0.92 0.06 0.09 0.09 0.85 0.75 0.82 0.73 0.7 0.68
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.11 0.08 0 0 0 0.1 0.09 0.09 0.14 0.1 0.07 0.06 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.09 0 0 0 0 0.06 0.09 0.08 0.07 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0.11 0.1 0.11
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.08 0.15 0.24 0.12 0.12 0.09 0.05 0.13 0 0.06 0.08 0.12 0.11 0.09 0.22 0.23 0.24 0 0.07 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 3 12 6 12 0 4 3 3 4 3 3 0 3 8 1 5 1 1 1 1 18 22
Bud 4 6 6 7 13 10 19 7 21 7 25 3 1 2 5 3 0 0 1 0 0 0 2
Bud Neck 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0 2 1
Bud Site 1 1 0 0 1 3 8 2 9 0 2 0 0 0 1 0 0
Cell Periphery 0 8 15 15 9 3 4 3 7 7 6 5 8 7 17 14 14 0 0 0 0 1 1
Cytoplasm 1 2 1 3 11 2 8 22 11 13 5 2 4 6 13 5 5 0 0 0 0 0 0
Endoplasmic Reticulum 1 5 4 4 3 4 2 2 6 4 10 2 4 6 4 4 3 0 0 0 0 1 1
Endosome 8 3 11 6 29 17 38 60 4 2 11 0 6 1 9 4 2 4 6 21 5 13 14
Golgi 1 7 61 30 57 4 4 14 1 2 13 6 25 48 119 83 85 6 16 20 4 22 22
Mitochondria 267 194 341 368 430 574 701 514 656 713 611 280 365 323 14 12 12 245 162 378 102 258 263
Nucleus 1 1 2 1 3 10 18 16 9 8 6 2 0 0 3 0 0 0 1 0 1 0 0
Nuclear Periphery 33 17 7 9 23 63 75 70 100 79 49 19 16 8 6 4 4 5 1 1 0 2 4
Nucleolus 27 7 3 5 15 42 78 63 53 46 31 7 5 5 8 4 1 1 2 1 1 2 1
Peroxisomes 1 3 8 6 8 3 8 10 1 2 5 6 6 8 8 1 7 15 5 13 15 35 41
SpindlePole 4 0 5 1 13 12 24 33 13 10 22 1 1 0 1 0 2 1 1 1 0 1 1
Vac/Vac Membrane 23 32 100 50 64 61 46 98 30 46 58 35 43 30 50 31 34 4 14 17 5 7 8
Unique Cell Count 288 214 421 400 522 652 842 750 714 793 689 299 395 352 232 133 139 289 217 463 140 370 390
Labelled Cell Count 373 289 577 511 691 808 1038 917 924 943 857 371 484 447 266 166 174 289 217 463 140 370 390


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.7 8.6 8.0 7.4 7.8 6.5 6.6 7.2 5.8 5.7 6.3 8.7 8.6 8.8 11.9 12.0 11.6 7.9 8.1 8.9
Std Deviation (1e-4) 1.6 1.6 1.2 1.2 1.3 1.1 1.1 1.2 1.1 1.1 1.3 1.4 1.3 1.3 2.4 2.2 2.3 1.5 1.5 1.6
Intensity Change (Log2) -0.1 -0.02 -0.29 -0.27 -0.15 -0.46 -0.49 -0.32 0.12 0.11 0.14 0.59 0.59 0.55 -0.01 0.03 0.17


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -1.3 -0.5 0 -3.6 -3.6 -3.5 -3.4 -3.4 -1.7 -3.4 -2.0 0.4 0 0
Bud 0.4 1.2 0.1 1.0 0 1.6 0 2.2 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.1 -1.8 -3.9 -4.2 -4.2 -3.0 -3.3 -3.2 -1.5 -1.3 -1.3 2.1 3.1 3.0
Cytoplasm 0 2.5 0 0 3.2 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0.7 0 0 0 0 0 0
Endosome -1.1 2.2 0 1.6 3.7 -2.9 0 -1.2 0 -1.1 -2.6 0.9 0 0
Golgi -3.2 -1.6 -9.3 -10.6 -8.5 -10.3 -10.6 -8.2 -5.7 -3.8 -0.3 10.1 11.0 10.9
Mitochondria 4.6 0.5 3.2 1.0 -4.6 5.4 4.4 3.6 4.9 4.8 4.3 -18.4 -15.0 -15.3
Nucleus 0 0 0 2.2 2.2 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.6 2.4 5.2 4.9 5.1 6.9 5.4 4.0 3.3 2.1 0.6 0 0 0
Nucleolus 0 2.4 4.6 5.8 5.5 5.0 4.3 3.6 0 0 0 0 0 0
Peroxisomes -0.4 -0.4 0 -1.4 -0.8 0 0 0 0.1 -0.4 0.4 1.2 0 0
SpindlePole 0 1.5 0.8 1.9 3.0 0.8 0.1 2.1 0 0 0 0 0 0
Vacuole -4.2 -4.6 -6.4 -9.6 -4.7 -10.0 -9.2 -7.1 -4.1 -4.8 -5.6 -0.6 -0.1 0.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.1192 4.1691 3.6994 4.0977 2.666 3.9454 5.3571 5.7226 5.4405 5.0982 4.937 5.3652 6.1162 6.1288 6.3134 5.7734 5.5715 6.0891
Actin 0.0202 0.0068 0.0043 0.0079 0.0071 0.0014 0.0166 0.0087 0.0105 0.0028 0.0045 0.0011 0.0165 0.0045 0.0089 0.0114 0.011 0.0058
Bud 0.0005 0.0002 0.0003 0.0003 0.0006 0.0001 0.0004 0.0016 0.0006 0.0034 0.0001 0 0.0003 0.0002 0.0002 0.0022 0.0002 0.0001
Bud Neck 0.0009 0.0002 0.0003 0 0.0003 0 0.0005 0.0002 0.0001 0.0001 0.0001 0 0.0003 0.0001 0.0001 0.0002 0.0002 0.0001
Bud Periphery 0.0009 0.0005 0.0006 0.0009 0.0017 0.0002 0.0008 0.0035 0.0017 0.0091 0.0003 0.0001 0.0004 0.0003 0.0003 0.0027 0.0005 0.0001
Bud Site 0.0025 0.0006 0.0013 0.0002 0.0013 0.0001 0.0046 0.0013 0.0012 0.0023 0.0002 0 0.002 0.0004 0.0005 0.0063 0.0003 0.0002
Cell Periphery 0.0004 0.0001 0.0002 0.0001 0.0002 0.0001 0.0003 0.0006 0.0002 0.0003 0.0001 0 0.0002 0.0002 0.0002 0.0002 0.0001 0
Cytoplasm 0.0029 0.0001 0.002 0 0.0007 0.0001 0.0012 0.0001 0.0002 0.0001 0.0004 0.0001 0.0047 0.0001 0.0011 0.0017 0.0002 0.0002
Cytoplasmic Foci 0.0213 0.0038 0.0078 0.0039 0.0105 0.0033 0.0108 0.0013 0.0039 0.0023 0.0118 0.0032 0.0258 0.0051 0.0022 0.0058 0.007 0.0044
Eisosomes 0.0011 0.0002 0.0002 0.0008 0.0004 0.0001 0.0004 0.0001 0.0002 0.0001 0.0001 0.0001 0.0003 0.0002 0.0003 0.0002 0.0002 0.0002
Endoplasmic Reticulum 0.0014 0.0001 0.0007 0.0002 0.0017 0.0001 0.0012 0 0.0004 0 0.0016 0.0001 0.0011 0.0001 0.0012 0.0009 0.0013 0.0002
Endosome 0.0257 0.0249 0.0183 0.0042 0.0248 0.0076 0.0331 0.0047 0.0098 0.005 0.0298 0.0083 0.0305 0.0113 0.0078 0.0122 0.0369 0.0048
Golgi 0.0283 0.0458 0.0161 0.0085 0.0233 0.0093 0.0307 0.0081 0.0088 0.0053 0.0306 0.0178 0.0214 0.035 0.0147 0.0098 0.0648 0.0139
Lipid Particles 0.0157 0.004 0.012 0.0019 0.0147 0.0081 0.0211 0.0009 0.0043 0.0134 0.0211 0.0064 0.0424 0.0314 0.0039 0.0308 0.0283 0.009
Mitochondria 0.8432 0.8949 0.8994 0.947 0.883 0.9491 0.8526 0.9497 0.9426 0.9444 0.8855 0.9554 0.826 0.8738 0.9405 0.9017 0.8379 0.953
None 0.0022 0 0.0014 0.0001 0.0045 0.0001 0.0021 0 0.0001 0.0001 0.0005 0 0.0015 0 0.0005 0.001 0.0002 0.0003
Nuclear Periphery 0.0009 0.0001 0.0084 0 0.0086 0.0002 0.0023 0 0.0005 0 0.0014 0.0001 0.0014 0.0002 0.0011 0.0012 0.0013 0.0004
Nucleolus 0.0003 0.0001 0.0007 0 0.0007 0.0001 0.0005 0.0043 0.0002 0.0001 0.0001 0 0.0005 0.0002 0.0008 0.0002 0.0001 0.0001
Nucleus 0.0005 0.0001 0.0034 0 0.0013 0.0001 0.0005 0.0007 0.0001 0.0001 0.0001 0 0.0008 0.0001 0.0004 0.0008 0.0002 0.0001
Peroxisomes 0.0176 0.0109 0.0078 0.0228 0.0065 0.0065 0.0094 0.0069 0.0087 0.0101 0.007 0.0052 0.0154 0.0202 0.0089 0.0064 0.0052 0.0026
Punctate Nuclear 0.0027 0 0.0027 0 0.0012 0.0003 0.0006 0 0.0001 0 0.0006 0 0.0021 0 0.0004 0.0006 0.0005 0.0029
Vacuole 0.0039 0.003 0.0059 0.0004 0.0024 0.0033 0.0034 0.0047 0.0017 0.0005 0.001 0.0004 0.0025 0.006 0.0035 0.0021 0.0011 0.0003
Vacuole Periphery 0.0069 0.0035 0.0061 0.0008 0.0045 0.01 0.007 0.0025 0.004 0.0005 0.0032 0.0016 0.0039 0.0107 0.0027 0.0017 0.0024 0.0013

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 29.456 22.7735 26.7566 31.9709 26.1488 19.6264 35.139 36.2627 31.6889 37.0485
Translational Efficiency 0.5658 0.7882 0.722 0.6085 0.5806 1.1296 0.6971 0.7459 0.9728 0.7881

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
576 456 82 1342 507 85 276 274 1083 541 358 1616

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 817.67 1002.31 1459.46 1168.87 829.24 1095.28 1223.21 1229.38 823.09 1016.92 1277.32 1179.13
Standard Deviation 143.92 185.21 222.68 149.90 181.49 166.69 149.15 197.65 162.70 185.54 195.87 160.62
Intensity Change Log 2 0.293738 0.835844 0.515524 0.401438 0.560810 0.568069 0.348971 0.703908 0.542220

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.004181 0.011197 0.017090 0.003206 0.001454 0.005201 0.002597 0.010278 0.002905 0.010255 0.005916 0.004405
Bud Neck 0.001782 0.002449 0.012893 0.002658 0.005853 0.003282 0.006776 0.003175 0.003688 0.002580 0.008177 0.002746
Bud Site 0.008595 0.003588 0.008974 0.009019 0.013425 0.004960 0.010478 0.018047 0.010856 0.003804 0.010133 0.010550
Cell Periphery 0.000529 0.000870 0.000290 0.000406 0.001174 0.001318 0.000234 0.001099 0.000831 0.000940 0.000247 0.000523
Cytoplasm 0.001020 0.000852 0.000989 0.000808 0.005675 0.000652 0.001169 0.001548 0.003199 0.000821 0.001128 0.000934
Cytoplasmic Foci 0.010613 0.029073 0.066698 0.016870 0.014000 0.033102 0.039614 0.025509 0.012198 0.029706 0.045818 0.018334
Eisosomes 0.000069 0.000238 0.000191 0.000078 0.000075 0.000189 0.000057 0.000181 0.000072 0.000230 0.000087 0.000096
Endoplasmic Reticulum 0.000366 0.000536 0.000887 0.000635 0.000853 0.000867 0.000522 0.000905 0.000594 0.000588 0.000605 0.000680
Endosome 0.038801 0.034496 0.033253 0.021443 0.030394 0.044240 0.047950 0.030989 0.034865 0.036027 0.044584 0.023062
Golgi 0.026658 0.053622 0.049762 0.068065 0.023911 0.065329 0.060194 0.088791 0.025372 0.055461 0.057805 0.071579
Lipid Particles 0.003187 0.006273 0.011495 0.001809 0.005950 0.004491 0.003599 0.000804 0.004481 0.005993 0.005408 0.001639
Mitochondria 0.858452* 0.804094* 0.619241* 0.811760* 0.848749* 0.771981* 0.704742* 0.749677* 0.853910* 0.799049* 0.685158* 0.801233*
Mitotic Spindle 0.002396 0.006214 0.075694 0.011759 0.002666 0.010904 0.050711 0.012721 0.002522 0.006951 0.056433 0.011922
None 0.000358 0.000431 0.000934 0.000585 0.000479 0.000361 0.000471 0.000588 0.000415 0.000420 0.000577 0.000585
Nuclear Periphery 0.000249 0.000182 0.003802 0.000824 0.000762 0.000268 0.002061 0.000190 0.000489 0.000195 0.002460 0.000716
Nuclear Periphery Foci 0.001361 0.000281 0.002409 0.000579 0.000614 0.000274 0.001519 0.000253 0.001012 0.000280 0.001723 0.000523
Nucleolus 0.000097 0.000289 0.000959 0.000167 0.001039 0.000178 0.000258 0.000122 0.000538 0.000271 0.000418 0.000160
Nucleus 0.000108 0.000168 0.000321 0.000190 0.001335 0.000212 0.000227 0.000127 0.000682 0.000175 0.000248 0.000180
Peroxisomes 0.013733 0.035417 0.035685 0.003920 0.023878 0.026450 0.017104 0.025213 0.018482 0.034008 0.021360 0.007530
Vacuole 0.011626 0.005571 0.030030 0.018790 0.007209 0.006849 0.026527 0.016742 0.009558 0.005772 0.027330 0.018443
Vacuole Periphery 0.015818 0.004159 0.028401 0.026430 0.010504 0.018892 0.023190 0.013041 0.013330 0.006474 0.024384 0.024160

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.61 -1.52 0.66 3.39 1.65 -2.39 -2.05 -4.32 -1.99 -3.67 -3.51 -1.42 -1.65 2.87 0.73
Bud Neck -0.92 -4.94 -1.34 -0.34 4.50 1.08 -0.37 1.24 0.07 1.99 0.98 -2.64 0.81 -0.31 3.67
Bud Site 2.25 -0.16 -0.29 -2.93 -0.11 2.31 0.74 -0.88 -2.64 -1.47 3.45 0.26 0.03 -3.82 -0.25
Cell Periphery -0.75 2.58 1.33 1.04 -1.72 -0.16 4.37 0.21 0.23 -2.88 -0.26 5.13 2.46 1.03 -3.72
Cytoplasm 0.44 0.09 0.56 0.06 0.68 2.18 1.94 1.78 -2.89 -0.86 2.15 1.84 2.04 -0.68 0.51
Cytoplasmic Foci -5.20 -4.68 -3.20 3.43 4.15 -2.95 -4.67 -2.27 0.97 2.01 -5.56 -6.74 -3.37 3.51 5.40
Eisosomes -4.93 -4.71 -0.97 4.72 4.45 -1.33 2.30 -5.19 0.09 -6.32 -5.02 -1.72 -3.09 4.26 -0.90
Endoplasmic Reticulum -1.25 -3.50 -2.72 -0.69 1.57 -0.04 1.37 -0.15 -0.10 -1.39 0.04 -0.09 -0.64 -0.69 -0.72
Endosome 0.73 0.67 3.65 3.13 1.67 -1.50 -2.90 -0.13 1.39 2.63 -0.26 -1.91 3.68 3.39 4.76
Golgi -4.35 -2.60 -7.76 -2.11 -1.95 -2.65 -4.01 -5.86 -1.25 -2.09 -5.42 -4.51 -10.12 -2.48 -1.73
Lipid Particles -1.77 -2.28 1.50 2.79 2.71 0.62 1.49 3.58 1.91 4.06 -0.97 -0.74 3.28 3.16 3.65
Mitochondria 3.17 5.77 3.66 -0.47 -4.68 2.17 5.94 4.32 0.59 -1.55 3.67 8.29 4.96 -0.14 -5.71
Mitotic Spindle -1.72 -3.86 -5.85 -2.19 3.34 -1.45 -5.66 -2.47 -0.28 4.16 -2.03 -6.91 -5.80 -2.11 5.59
None -1.49 -5.57 -5.68 -3.37 3.41 1.65 0.18 -1.41 -2.72 -1.59 -0.10 -3.28 -4.76 -4.06 -0.21
Nuclear Periphery 0.46 -3.41 -3.38 -4.98 2.87 1.11 -2.12 1.29 1.12 4.36 1.30 -4.27 -0.97 -4.81 4.19
Nuclear Periphery Foci 0.86 -0.77 0.62 -2.98 3.73 1.97 -2.91 2.14 0.23 4.62 1.09 -1.00 0.72 -2.88 4.90
Nucleolus -1.10 -2.34 -1.17 0.65 2.11 1.64 1.52 1.79 0.41 1.68 0.90 0.46 1.52 0.71 2.31
Nucleus -0.80 -3.05 -1.34 -0.32 0.94 1.35 1.35 1.47 0.58 1.10 1.26 1.12 1.22 -0.18 0.53
Peroxisomes -3.57 -2.29 3.40 5.79 3.46 -0.21 1.34 -0.18 0.10 -1.33 -2.86 -0.70 4.22 5.32 4.01
Vacuole 2.47 -2.69 -2.71 -6.52 1.65 0.16 -4.56 -2.47 -2.32 1.74 2.35 -4.78 -4.62 -6.90 2.28
Vacuole Periphery 5.19 -1.59 -3.64 -11.00 0.25 -1.16 -3.70 -1.05 0.78 2.64 3.83 -3.36 -5.15 -8.55 0.07
Standard name
Human Ortholog
Description Multicopy suppressor of HER2 involved in mitochondrial translation; mutant is defective in directing meiotic recombination events to homologous chromatids
Localization
Cell Percentages mitochondrion (96%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Msc6

Msc6


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Msc6-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available