Standard name
Human Ortholog
Description Subunit of the Hap2p/3p/4p/5p CCAAT-binding complex; complex is heme-activated and glucose repressed; complex is a transcriptional activator and global regulator of respiratory gene expression; required for assembly and DNA binding activity of the complex
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.06 0.05 0 0 0 0 0 0 0 0 0.1 0.14 0.11 0.06 0.07 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.06 0.41 0 0 0 0.06 0 0.09 0.22 0.17 0.16 0.13 0 0 0 0.09 0.06 0.05 0 0 0 0 0 0
Nucleus 0.94 0.81 0.97 0.92 0.93 0.92 0.93 0.89 0.84 0.87 0.9 0.87 0.95 0.88 0.82 0.79 0.89 0.88 0.95 0.83 0.9 0.85 0.77 0.71
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.1 0.06 0 0 0 0 0 0 0.13 0.1 0.14 0.11 0 0 0 0 0 0 0 0 0 0 0 0.07
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0.09 0.14
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1 0
Bud 0 0 0 0 0 0 2 1 3 2 3 0 1 0 0 0 0 0 0 0 1 2 2 3
Bud Neck 0 0 1 0 1 0 0 3 3 2 1 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 2 2 5 11 5 13 9 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 3 4 12 12 14 9 18 15 14 8 3 5 36 59 6 9 12 0 0 0 1 7 2
Endoplasmic Reticulum 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 2 1 3 0 0 0 0 0 0
Endosome 0 0 0 1 0 3 3 0 0 1 0 0 1 2 5 0 2 0 0 1 4 1 5 2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 0 1 0
Mitochondria 3 28 3 4 0 22 18 54 97 79 60 57 7 10 6 5 9 9 0 1 1 1 7 3
Nucleus 49 56 188 192 228 351 400 536 374 400 331 373 173 319 336 42 132 151 64 71 183 129 259 214
Nuclear Periphery 0 0 0 1 0 0 4 6 1 1 1 2 0 5 2 0 2 0 0 0 0 0 0 1
Nucleolus 5 4 0 0 1 5 9 22 56 44 52 48 0 2 5 0 1 2 0 0 2 2 10 20
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 2 3 2 1 4 9 2 0 0 0 5 2 3 0 0 1 0 0 1 1 6 4
Vac/Vac Membrane 1 1 0 3 4 4 4 10 3 0 3 4 1 5 7 1 6 5 2 6 5 6 30 43
Unique Cell Count 52 69 193 209 244 380 431 600 446 458 367 429 182 363 410 53 149 171 68 86 203 152 336 301
Labelled Cell Count 58 92 198 216 248 402 456 664 565 548 472 496 194 381 423 56 163 183 68 86 203 152 336 301


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.2 4.3 6.1 6.0 6.8 5.9 5.7 5.8 5.2 5.5 5.5 5.8 5.6 6.5 5.9 6.4 7.1 8.0 6.4 6.9 6.5
Std Deviation (1e-4) 0.7 1.0 1.0 1.4 1.4 1.3 1.0 1.2 1.1 1.1 1.3 1.2 1.0 7.2 1.3 1.6 1.8 2.0 1.4 1.4 1.0
Intensity Change (Log2) – – – -0.01 0.17 -0.04 -0.09 -0.06 -0.23 -0.14 -0.14 -0.07 -0.12 0.1 -0.03 0.07 0.22 0.39 0.09 0.19 0.1


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.4582 0.6079 0.5344 -0.2123 0.0444 0.3452 1.8421 1.4741 1.4863 1.9269 1.5801 1.3695 -0.2006 0.1484 -0.2951 -0.1315 0.1121 -0.2207
Actin 0.0052 0 0.0315 0.0122 0.0209 0.0007 0.0313 0 0.0017 0 0.0001 0.0002 0.0066 0 0.0017 0.0503 0.0882 0.0052
Bud 0.0003 0 0.0001 0 0.0004 0 0.0005 0 0.0001 0 0 0 0.0004 0 0.0001 0.0012 0.0021 0.0001
Bud Neck 0.002 0.0001 0.0005 0.0001 0.0017 0.0007 0.0011 0 0.0002 0.0001 0.0004 0.0006 0.0015 0.0002 0.0002 0.0007 0.0008 0.0009
Bud Periphery 0.0009 0 0.0003 0 0.0005 0 0.0009 0 0.0001 0 0 0.0001 0.0006 0 0.0003 0.0015 0.0021 0.0001
Bud Site 0.0019 0.0001 0.0005 0.0001 0.0028 0 0.0073 0 0.0006 0 0.0001 0.0001 0.007 0.0001 0.0003 0.0072 0.0176 0.0001
Cell Periphery 0.0002 0 0.0001 0 0.0002 0 0.0006 0 0.0001 0 0 0 0.0005 0 0 0.0001 0.0003 0
Cytoplasm 0.003 0.0011 0.0019 0.0034 0.0033 0.0004 0.0452 0.0001 0.0051 0.0006 0.0018 0.0065 0.0097 0.0014 0.0024 0.0009 0.0038 0.0003
Cytoplasmic Foci 0.0142 0.0001 0.0018 0.0029 0.0058 0.0008 0.0167 0 0.025 0 0 0.0108 0.0183 0.001 0.0042 0.0021 0.013 0.0016
Eisosomes 0.0001 0 0.0002 0.0002 0.0005 0 0.0009 0 0.0001 0 0 0 0.0001 0 0 0.0002 0.0004 0
Endoplasmic Reticulum 0.0008 0.0001 0.0029 0.0006 0.0011 0 0.0424 0 0.0005 0 0.0001 0.0003 0.0021 0.0002 0.0004 0.0001 0.0096 0.0001
Endosome 0.0073 0.0002 0.0047 0.0049 0.0087 0.0006 0.0988 0 0.0093 0 0.0001 0.0023 0.0235 0.0008 0.0059 0.001 0.0761 0.0029
Golgi 0.0019 0 0.0019 0.001 0.0041 0.0013 0.0113 0 0.0034 0 0 0.0003 0.0069 0 0.008 0.0028 0.046 0.0088
Lipid Particles 0.0093 0 0.0032 0.002 0.007 0.0018 0.0229 0 0.0047 0 0 0.0084 0.0176 0.0004 0.009 0.0012 0.0052 0.0011
Mitochondria 0.0086 0.0002 0.0051 0.0004 0.0049 0.0006 0.0163 0.0001 0.0015 0.0001 0.0002 0.0003 0.0031 0.0001 0.004 0.0049 0.0216 0.0029
None 0.0014 0.0014 0.0008 0.017 0.0562 0.0085 0.0444 0 0.0088 0.0012 0.0014 0.0016 0.0036 0.0002 0.0009 0.0002 0.001 0.0238
Nuclear Periphery 0.0082 0.0111 0.0126 0.0083 0.0516 0.0019 0.1275 0.0004 0.0012 0.0017 0.0063 0.0029 0.0064 0.0094 0.0102 0.0028 0.004 0.0002
Nucleolus 0.0133 0.0095 0.0087 0.0097 0.0249 0.0186 0.0111 0.0037 0.01 0.0044 0.0215 0.0099 0.0068 0.0064 0.0045 0.0073 0.0048 0.0081
Nucleus 0.8938 0.9598 0.9144 0.9235 0.7726 0.9484 0.4617 0.9953 0.9019 0.9861 0.9388 0.9352 0.8623 0.9575 0.9443 0.9099 0.6932 0.9427
Peroxisomes 0.0024 0 0.0025 0.0017 0.008 0.0018 0.003 0 0.0082 0 0 0.0036 0.0057 0 0.0011 0.0045 0.0042 0.0007
Punctate Nuclear 0.0224 0.016 0.0049 0.0114 0.0199 0.0136 0.0328 0.0001 0.0168 0.0057 0.0289 0.0169 0.0086 0.0219 0.0013 0.0009 0.0016 0.0003
Vacuole 0.0019 0.0002 0.0009 0.0001 0.0032 0.0001 0.0113 0 0.0005 0 0.0001 0.0002 0.0073 0.0001 0.0004 0.0002 0.0023 0.0001
Vacuole Periphery 0.0009 0.0001 0.0006 0.0003 0.0019 0 0.012 0 0.0001 0 0 0.0001 0.0015 0.0001 0.0006 0.0002 0.0021 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 6.0543 9.4714 7.0282 8.9068 7.8497 10.5892 6.8404 15.697 11.8086 9.5759
Translational Efficiency 1.3538 0.6896 0.9557 1.3037 1.5958 1.042 1.4144 0.7147 1.1122 1.3579

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
439 987 2084 250 1826 2226 1781 149 2265 3213 3865 399

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 648.87 788.67 804.63 966.34 735.52 849.77 882.50 993.75 718.73 831.00 840.51 976.58
Standard Deviation 77.03 120.79 114.88 126.32 87.38 103.63 112.00 120.27 92.08 112.77 120.01 124.80
Intensity Change Log 2 — 0.281492 0.310396 0.574601 — 0.208308 0.262832 0.434118 — 0.243072 0.285321 0.501669

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000117 0.000556 0.000467 0.008533 0.000051 0.000097 0.000653 0.000705 0.000064 0.000238 0.000553 0.005609
Bud Neck 0.001229 0.008167 0.016373 0.010540 0.002272 0.004892 0.011012 0.008462 0.002070 0.005898 0.013902 0.009764
Bud Site 0.000283 0.002932 0.002071 0.005672 0.000219 0.000566 0.001904 0.000982 0.000231 0.001293 0.001994 0.003921
Cell Periphery 0.000121 0.000166 0.000092 0.000146 0.000067 0.000092 0.000113 0.000063 0.000078 0.000115 0.000101 0.000115
Cytoplasm 0.015873 0.024457 0.008624 0.013222 0.007270 0.009386 0.009947 0.002245 0.008937 0.014016 0.009233 0.009123
Cytoplasmic Foci 0.002012 0.005464 0.000092 0.002020 0.000403 0.000670 0.001020 0.000019 0.000715 0.002143 0.000520 0.001273
Eisosomes 0.000020 0.000028 0.000018 0.000059 0.000009 0.000010 0.000027 0.000024 0.000011 0.000016 0.000022 0.000046
Endoplasmic Reticulum 0.001570 0.005828 0.004970 0.009417 0.001454 0.002047 0.005333 0.005027 0.001477 0.003208 0.005137 0.007777
Endosome 0.000267 0.001431 0.000429 0.006319 0.000141 0.000396 0.001342 0.000153 0.000165 0.000714 0.000850 0.004016
Golgi 0.000075 0.000687 0.000049 0.007748 0.000020 0.000052 0.000559 0.000049 0.000031 0.000247 0.000284 0.004873
Lipid Particles 0.001035 0.001323 0.000344 0.000747 0.000164 0.000462 0.000348 0.000075 0.000333 0.000726 0.000346 0.000496
Mitochondria 0.000574 0.002800 0.001151 0.002920 0.000223 0.001318 0.001157 0.000811 0.000291 0.001773 0.001154 0.002132
Mitotic Spindle 0.000017 0.004082 0.001177 0.009484 0.000010 0.000906 0.007116 0.004684 0.000011 0.001881 0.003913 0.007691
None 0.009876 0.007466 0.002501 0.009179 0.003373 0.003129 0.006158 0.003350 0.004634 0.004461 0.004187 0.007002
Nuclear Periphery 0.000346 0.000778 0.001115 0.001712 0.000467 0.000785 0.001215 0.000374 0.000444 0.000783 0.001161 0.001212
Nuclear Periphery Foci 0.000229 0.001030 0.000260 0.000453 0.000128 0.000217 0.000348 0.000060 0.000148 0.000467 0.000300 0.000306
Nucleolus 0.011646 0.012700 0.002514 0.005537 0.005454 0.004001 0.004901 0.004819 0.006654 0.006673 0.003614 0.005269
Nucleus 0.953600* 0.916096* 0.955859* 0.873729* 0.977811* 0.969591* 0.942990* 0.966640* 0.973118* 0.953158* 0.949929* 0.908425*
Peroxisomes 0.000696 0.001343 0.000092 0.000389 0.000074 0.000168 0.000339 0.000133 0.000195 0.000529 0.000206 0.000294
Vacuole 0.000321 0.002106 0.001710 0.026310 0.000334 0.000943 0.002795 0.001243 0.000332 0.001300 0.002210 0.016949
Vacuole Periphery 0.000094 0.000559 0.000093 0.005866 0.000054 0.000274 0.000723 0.000081 0.000061 0.000362 0.000383 0.003706

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -9.37 -16.85 -1.90 -1.66 -1.68 -3.53 -22.34 -7.95 -7.55 -2.62 -10.01 -30.13 -2.28 -2.13 -1.78
Bud Neck -5.95 -16.96 -10.10 -4.53 1.38 -3.42 -15.18 -6.74 -4.91 -0.55 -5.99 -21.90 -11.87 -7.64 0.49
Bud Site -2.85 -4.86 -5.70 -2.31 -3.59 -2.92 -8.35 -4.26 -3.46 -0.52 -3.70 -8.85 -6.86 -4.77 -3.61
Cell Periphery -1.41 1.34 -0.47 1.21 -2.84 -1.22 -3.61 -0.98 0.19 1.11 -2.23 -2.34 -2.81 -0.07 -1.42
Cytoplasm -2.06 2.23 1.70 5.12 -0.90 -1.66 -1.56 3.72 5.33 5.15 -3.64 0.18 1.36 4.96 1.48
Cytoplasmic Foci -2.01 3.12 2.26 3.80 -1.77 -1.76 -1.19 4.12 4.48 2.64 -3.16 2.05 1.56 3.99 -0.12
Eisosomes -2.49 0.70 -5.57 -4.70 -6.07 -2.17 -19.57 -6.47 -6.06 -1.11 -5.20 -15.71 -8.61 -7.61 -5.83
Endoplasmic Reticulum -10.08 -15.08 -10.84 -6.03 -6.96 -2.28 -20.35 -7.75 -6.54 -0.75 -7.68 -24.88 -12.87 -9.88 -6.46
Endosome -2.78 -0.13 -2.30 -0.96 -2.28 -1.85 -2.93 -3.11 0.57 2.37 -3.60 -3.24 -2.66 -1.58 -1.56
Golgi -2.75 1.26 -1.50 -1.06 -1.53 -2.30 -2.10 -4.46 -2.17 1.61 -3.24 -2.14 -1.58 -1.33 -1.36
Lipid Particles -0.34 1.89 1.32 1.80 -1.33 -1.52 -2.42 0.18 1.55 2.41 -1.87 0.28 -0.59 1.56 -0.82
Mitochondria -3.03 -7.87 -5.29 0.66 -3.07 -2.91 -11.27 -8.70 0.18 0.18 -4.31 -18.93 -7.96 -0.31 -2.89
Mitotic Spindle -2.89 -3.63 -3.50 -2.80 -3.26 -1.82 -4.86 -1.55 -1.34 -0.10 -3.39 -5.81 -3.81 -3.36 -2.91
None 1.16 4.17 1.48 0.62 -4.13 0.50 -2.21 -0.31 -0.64 1.36 0.24 1.39 -1.14 -1.46 -2.35
Nuclear Periphery -5.85 -8.76 -5.02 -4.10 -3.06 -3.11 -2.34 -2.15 -0.36 1.15 -4.47 -4.74 -5.14 -3.99 -2.13
Nuclear Periphery Foci -2.03 0.21 -1.64 1.47 -1.94 -0.34 -1.85 -0.25 0.08 1.33 -1.90 -1.25 -2.00 0.60 -1.01
Nucleolus -0.61 6.34 3.03 3.67 -1.62 2.83 0.12 0.19 -1.15 0.12 -0.12 5.23 1.10 1.16 -1.25
Nucleus 4.46 -0.18 5.50 2.85 6.13 3.24 11.31 3.71 2.58 -1.07 7.05 10.96 8.15 5.70 5.08
Peroxisomes -0.05 1.61 1.33 3.35 -3.88 -3.32 -2.35 -2.95 -0.83 0.62 -1.25 0.89 0.28 2.53 -1.70
Vacuole -1.22 -1.24 -4.60 -4.44 -4.44 -2.22 -8.19 -2.35 -1.95 -0.18 -3.07 -7.75 -5.01 -4.77 -4.31
Vacuole Periphery -3.00 0.07 -1.55 -1.10 -1.56 -2.62 -1.98 -4.84 1.89 1.73 -4.00 -2.11 -1.62 -1.15 -1.23
Standard name
Human Ortholog
Description Subunit of the Hap2p/3p/4p/5p CCAAT-binding complex; complex is heme-activated and glucose repressed; complex is a transcriptional activator and global regulator of respiratory gene expression; required for assembly and DNA binding activity of the complex
Localization
Cell Percentages nucleus (98%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Hap5

Hap5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Hap5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available