Standard name | |
---|---|
Human Ortholog | |
Description | eIF3b subunit of the eukaryotic translation initiation factor 3 (eIF3); subunit of the core complex of eIF3; essential for translation; part of a subcomplex (Prt1p-Rpg1p-Nip1p) that stimulates binding of mRNA and tRNA(i)Met to ribosomes; eIF3 is also involved in programmed stop codon readthrough |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 1 | 0 | 0 | 2 | 9 | – | 18 | 17 | 18 | 18 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 2 | 6 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 8 | 10 | 10 | 9 | 16 | – | 18 | 17 | 16 | 30 | 3 | 2 | 2 | 4 | 3 | 8 | 0 | 0 | 0 | 1 | 0 | 0 |
Cytoplasm | 113 | 282 | 265 | 200 | 211 | 171 | 245 | – | 168 | 136 | 135 | 138 | 274 | 378 | 400 | 246 | 223 | 147 | 109 | 276 | 268 | 176 | 294 | 297 |
Endoplasmic Reticulum | 0 | 2 | 7 | 6 | 11 | 24 | 46 | – | 40 | 41 | 37 | 40 | 12 | 17 | 14 | 5 | 8 | 5 | 0 | 2 | 5 | 24 | 41 | 58 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 2 | 6 | – | 4 | 9 | 5 | 19 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 1 |
Mitochondria | 0 | 0 | 0 | 1 | 0 | 2 | 3 | – | 14 | 21 | 23 | 22 | 1 | 0 | 0 | 2 | 2 | 1 | 0 | 1 | 1 | 1 | 2 | 3 |
Nucleus | 2 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 2 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 1 | 3 | 0 | 2 | 0 | 0 | – | 3 | 6 | 3 | 2 | 1 | 0 | 0 | 0 | 1 | 2 | 1 | 4 | 6 | 4 | 5 | 7 |
Unique Cell Count | 114 | 284 | 273 | 213 | 224 | 201 | 303 | 235 | 219 | 205 | 222 | 288 | 393 | 410 | 250 | 227 | 156 | 115 | 293 | 288 | 213 | 354 | 380 | |
Labelled Cell Count | 115 | 287 | 284 | 217 | 234 | 210 | 326 | 266 | 249 | 239 | 271 | 291 | 398 | 416 | 257 | 237 | 166 | 115 | 293 | 288 | 213 | 354 | 380 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 64.7 | 74.0 | 50.0 | 48.9 | 41.7 | 34.5 | 31.4 | – | 27.2 | 25.6 | 24.9 | 24.8 | 60.8 | 56.3 | 53.2 | 65.4 | 65.9 | 51.2 | 55.5 | 56.2 | 57.5 |
Std Deviation (1e-4) | 15.5 | 14.8 | 12.9 | 11.3 | 7.9 | 7.4 | 6.9 | – | 6.5 | 6.3 | 5.3 | 5.3 | 12.4 | 11.1 | 11.1 | 19.5 | 20.3 | 19.1 | 11.6 | 12.5 | 13.7 |
Intensity Change (Log2) | – | – | – | -0.03 | -0.26 | -0.53 | -0.67 | – | -0.88 | -0.97 | -1.01 | -1.01 | 0.28 | 0.17 | 0.09 | 0.39 | 0.4 | 0.03 | 0.15 | 0.17 | 0.2 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 4.3 | 4.3 | 4.7 | 4.5 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 1.0 | 0.9 | 0.9 | 1.4 | – | 2.4 | 2.4 | 2.4 | 4.4 | -1.6 | 0 | 0 | -1.0 | 0 | 1.2 |
Cytoplasm | -1.7 | -1.6 | -4.8 | -6.1 | – | -8.1 | -9.9 | -9.1 | -9.9 | -1.2 | -0.6 | 0.4 | 1.0 | 0.8 | -1.4 |
Endoplasmic Reticulum | 0.2 | 1.4 | 4.1 | 5.2 | – | 5.6 | 6.0 | 5.8 | 5.8 | 1.0 | 1.2 | 0.6 | -0.4 | 0.6 | 0 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 4.9 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | – | 4.1 | 5.2 | 5.7 | 5.3 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 41.9178 | 63.6185 | 55.9795 | 50.6025 | 38.3457 | 56.654 | 50.0956 | 74.1437 | 69.0416 | 67.3302 | 59.1364 | 62.314 | 47.5397 | 72.567 | 67.1211 | 62.3479 | 42.0469 | 65.8748 |
Actin | 0.0043 | 0.0002 | 0.0014 | 0.0011 | 0.0015 | 0.0011 | 0.0041 | 0.0002 | 0.0078 | 0.0004 | 0.0019 | 0.0021 | 0.0294 | 0.0021 | 0.014 | 0.0005 | 0.0215 | 0.0044 |
Bud | 0.0014 | 0.0003 | 0.0015 | 0.0019 | 0.0017 | 0.0005 | 0.0018 | 0.0002 | 0.0021 | 0.0022 | 0.0019 | 0.0017 | 0.0014 | 0.0005 | 0.0007 | 0.0012 | 0.0044 | 0.0018 |
Bud Neck | 0.0005 | 0.0004 | 0.0006 | 0.0006 | 0.0009 | 0.0014 | 0.0009 | 0.0002 | 0.0006 | 0.0006 | 0.0004 | 0.0014 | 0.0078 | 0.0003 | 0.002 | 0.0007 | 0.0124 | 0.0057 |
Bud Periphery | 0.0017 | 0.0003 | 0.0015 | 0.0018 | 0.0037 | 0.0005 | 0.0026 | 0.0001 | 0.0024 | 0.0017 | 0.0016 | 0.002 | 0.0022 | 0.0005 | 0.0009 | 0.0003 | 0.0097 | 0.002 |
Bud Site | 0.002 | 0.0003 | 0.0103 | 0.0065 | 0.0004 | 0.0001 | 0.0023 | 0.0002 | 0.0009 | 0.0004 | 0.0006 | 0.0002 | 0.0101 | 0.0049 | 0.0028 | 0.0003 | 0.002 | 0.0028 |
Cell Periphery | 0.0049 | 0.0061 | 0.011 | 0.0124 | 0.0027 | 0.0005 | 0.0053 | 0.003 | 0.0058 | 0.0018 | 0.0032 | 0.0009 | 0.0082 | 0.0067 | 0.0029 | 0.0012 | 0.0034 | 0.0066 |
Cytoplasm | 0.6208 | 0.9055 | 0.751 | 0.6799 | 0.5976 | 0.7964 | 0.7075 | 0.9545 | 0.865 | 0.8156 | 0.7848 | 0.8573 | 0.676 | 0.9522 | 0.8992 | 0.8132 | 0.602 | 0.8505 |
Cytoplasmic Foci | 0.015 | 0.0009 | 0.0074 | 0.0223 | 0.0236 | 0.0056 | 0.0209 | 0.001 | 0.0078 | 0.0146 | 0.0216 | 0.0128 | 0.0259 | 0.0016 | 0.0028 | 0.0064 | 0.0181 | 0.0087 |
Eisosomes | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0003 | 0 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0008 | 0 | 0.0007 | 0.0001 | 0.0002 | 0.0005 | 0.0002 | 0 |
Endoplasmic Reticulum | 0.0667 | 0.0469 | 0.0575 | 0.0104 | 0.0305 | 0.0755 | 0.0229 | 0.0114 | 0.0155 | 0.0081 | 0.0118 | 0.0236 | 0.0553 | 0.0053 | 0.0064 | 0.0037 | 0.0159 | 0.0326 |
Endosome | 0.0196 | 0.0021 | 0.0131 | 0.0265 | 0.0398 | 0.0168 | 0.0254 | 0.0013 | 0.0071 | 0.0076 | 0.0256 | 0.0198 | 0.0331 | 0.0022 | 0.0106 | 0.0148 | 0.035 | 0.0311 |
Golgi | 0.002 | 0.0003 | 0.005 | 0.0043 | 0.0054 | 0.009 | 0.0032 | 0.0002 | 0.0021 | 0.0004 | 0.002 | 0.0031 | 0.0068 | 0.0012 | 0.0036 | 0.0007 | 0.0058 | 0.0071 |
Lipid Particles | 0.0093 | 0.0006 | 0.0043 | 0.003 | 0.0157 | 0.0168 | 0.0122 | 0.0008 | 0.0015 | 0.0023 | 0.015 | 0.0017 | 0.0125 | 0.0008 | 0.0012 | 0.0029 | 0.0024 | 0.0013 |
Mitochondria | 0.0159 | 0.0011 | 0.0158 | 0.0356 | 0.0606 | 0.0446 | 0.0219 | 0.0001 | 0.0127 | 0.003 | 0.0316 | 0.007 | 0.0094 | 0.0007 | 0.0134 | 0.0157 | 0.0172 | 0.0084 |
None | 0.0054 | 0.0002 | 0.0043 | 0.0029 | 0.0049 | 0.0003 | 0.0097 | 0.0002 | 0.0012 | 0.0092 | 0.0367 | 0.0051 | 0.0091 | 0.0006 | 0.0004 | 0.0057 | 0.0314 | 0.0005 |
Nuclear Periphery | 0.0343 | 0.004 | 0.0115 | 0.0178 | 0.0131 | 0.0065 | 0.0241 | 0.0017 | 0.0044 | 0.0043 | 0.0063 | 0.008 | 0.0267 | 0.0027 | 0.003 | 0.0059 | 0.0579 | 0.0064 |
Nucleolus | 0.015 | 0.0001 | 0.002 | 0.0005 | 0.0021 | 0.0001 | 0.0046 | 0.0001 | 0.0006 | 0.0011 | 0.0035 | 0.0009 | 0.0023 | 0.0001 | 0.0003 | 0.0103 | 0.0036 | 0.0002 |
Nucleus | 0.0289 | 0.0016 | 0.0063 | 0.0038 | 0.0054 | 0.0019 | 0.0347 | 0.0011 | 0.0022 | 0.0024 | 0.0038 | 0.0042 | 0.009 | 0.0018 | 0.002 | 0.0053 | 0.0826 | 0.0027 |
Peroxisomes | 0.0008 | 0 | 0.0005 | 0.0005 | 0.0015 | 0.0004 | 0.0069 | 0 | 0.0005 | 0.0005 | 0.0016 | 0.0001 | 0.0027 | 0.0001 | 0.0011 | 0.0008 | 0.0014 | 0.0008 |
Punctate Nuclear | 0.011 | 0 | 0.004 | 0.0002 | 0.0008 | 0.0001 | 0.0072 | 0 | 0.0001 | 0.0004 | 0.0011 | 0.0014 | 0.0113 | 0.0006 | 0.0004 | 0.001 | 0.0313 | 0.0007 |
Vacuole | 0.1065 | 0.0264 | 0.0784 | 0.1369 | 0.1282 | 0.0169 | 0.0689 | 0.0228 | 0.0518 | 0.1051 | 0.0307 | 0.0323 | 0.0463 | 0.0124 | 0.0288 | 0.0858 | 0.0291 | 0.0192 |
Vacuole Periphery | 0.0338 | 0.0027 | 0.0124 | 0.0312 | 0.0598 | 0.0048 | 0.0129 | 0.0011 | 0.0077 | 0.0182 | 0.0133 | 0.0143 | 0.0137 | 0.0025 | 0.0033 | 0.023 | 0.0125 | 0.0068 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 185.345 | 194.0906 | 180.4287 | 146.4261 | 177.2661 | 178.9874 | 155.5599 | 130.3962 | 129.9995 | 160.0677 |
Translational Efficiency | 1.8497 | 1.9241 | 1.5536 | 1.9197 | 1.6717 | 1.5706 | 1.6989 | 1.6191 | 1.5487 | 1.6044 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1813 | 981 | 1684 | 1385 | 2263 | 1775 | 2131 | 255 | 4076 | 2756 | 3815 | 1640 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 3336.20 | 3764.76 | 5248.37 | 6449.29 | 3703.08 | 3723.38 | 5594.64 | 6567.65 | 3539.89 | 3738.11 | 5441.79 | 6467.69 |
Standard Deviation | 615.66 | 940.27 | 888.29 | 1252.86 | 664.18 | 784.76 | 947.28 | 1162.45 | 668.40 | 843.64 | 937.60 | 1239.97 |
Intensity Change Log 2 | — | 0.174352 | 0.653664 | 0.950935 | — | 0.007887 | 0.595320 | 0.826652 | — | 0.089180 | 0.623266 | 0.886891 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000030 | 0.000140 | 0.000064 | 0.000238 | 0.000032 | 0.000060 | 0.000058 | 0.000154 | 0.000031 | 0.000088 | 0.000061 | 0.000225 |
Bud Neck | 0.000100 | 0.000558 | 0.000741 | 0.000879 | 0.000089 | 0.000307 | 0.000921 | 0.002276 | 0.000094 | 0.000396 | 0.000841 | 0.001096 |
Bud Site | 0.002136 | 0.004358 | 0.002004 | 0.006068 | 0.001664 | 0.002623 | 0.003805 | 0.012712 | 0.001874 | 0.003240 | 0.003010 | 0.007101 |
Cell Periphery | 0.003760 | 0.009344 | 0.013113 | 0.068248 | 0.005589 | 0.004626 | 0.003809 | 0.023167 | 0.004775 | 0.006305 | 0.007916 | 0.061238 |
Cytoplasm | 0.801084* | 0.750162* | 0.708565* | 0.582154* | 0.811921* | 0.849634* | 0.716613* | 0.425926* | 0.807101* | 0.814227* | 0.713061* | 0.557863* |
Cytoplasmic Foci | 0.010273 | 0.011222 | 0.005183 | 0.002691 | 0.015452 | 0.009661 | 0.006201 | 0.010403 | 0.013149 | 0.010216 | 0.005752 | 0.003890 |
Eisosomes | 0.000000 | 0.000002 | 0.000001 | 0.000005 | 0.000001 | 0.000001 | 0.000001 | 0.000003 | 0.000001 | 0.000001 | 0.000001 | 0.000005 |
Endoplasmic Reticulum | 0.000912 | 0.001159 | 0.000993 | 0.000586 | 0.002303 | 0.000864 | 0.000373 | 0.000302 | 0.001684 | 0.000969 | 0.000646 | 0.000542 |
Endosome | 0.000534 | 0.001249 | 0.000506 | 0.000621 | 0.000784 | 0.000821 | 0.000814 | 0.002802 | 0.000673 | 0.000974 | 0.000678 | 0.000960 |
Golgi | 0.052528 | 0.077364 | 0.036336 | 0.014640 | 0.060394 | 0.050741 | 0.023544 | 0.049209 | 0.056895 | 0.060217 | 0.029190 | 0.020015 |
Lipid Particles | 0.002687 | 0.010643 | 0.009610 | 0.034319 | 0.006820 | 0.006633 | 0.006003 | 0.029219 | 0.004982 | 0.008060 | 0.007595 | 0.033526 |
Mitochondria | 0.000216 | 0.000142 | 0.000159 | 0.000287 | 0.000201 | 0.000209 | 0.000225 | 0.001963 | 0.000208 | 0.000185 | 0.000196 | 0.000547 |
Mitotic Spindle | 0.000670 | 0.001440 | 0.001036 | 0.000580 | 0.000691 | 0.001034 | 0.001210 | 0.001030 | 0.000682 | 0.001178 | 0.001133 | 0.000650 |
None | 0.001524 | 0.002724 | 0.001440 | 0.002174 | 0.001365 | 0.002187 | 0.000718 | 0.000627 | 0.001436 | 0.002378 | 0.001036 | 0.001933 |
Nuclear Periphery | 0.000202 | 0.000464 | 0.001001 | 0.000418 | 0.000250 | 0.000198 | 0.000429 | 0.000693 | 0.000229 | 0.000293 | 0.000681 | 0.000460 |
Nuclear Periphery Foci | 0.005906 | 0.016392 | 0.013609 | 0.005244 | 0.009640 | 0.010971 | 0.008265 | 0.010591 | 0.007979 | 0.012901 | 0.010624 | 0.006075 |
Nucleolus | 0.000030 | 0.000378 | 0.000075 | 0.000181 | 0.000023 | 0.000056 | 0.000126 | 0.000172 | 0.000026 | 0.000170 | 0.000104 | 0.000180 |
Nucleus | 0.002385 | 0.006129 | 0.007862 | 0.006270 | 0.001307 | 0.003308 | 0.009683 | 0.007654 | 0.001787 | 0.004312 | 0.008879 | 0.006485 |
Peroxisomes | 0.000012 | 0.000068 | 0.000019 | 0.000159 | 0.000023 | 0.000019 | 0.000030 | 0.000107 | 0.000018 | 0.000037 | 0.000025 | 0.000151 |
Vacuole | 0.017690 | 0.047768 | 0.123128 | 0.243201* | 0.023378 | 0.026836 | 0.162927 | 0.345040* | 0.020848 | 0.034287 | 0.145359 | 0.259035* |
Vacuole Periphery | 0.097321 | 0.058297 | 0.074554 | 0.031039 | 0.058072 | 0.029211 | 0.054245 | 0.075949 | 0.075530 | 0.039564 | 0.063210 | 0.038022 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -4.44 | -5.47 | -6.33 | -2.10 | -4.94 | -3.90 | -6.20 | -3.69 | -2.99 | -2.66 | -5.74 | -8.28 | -6.99 | -4.57 | -5.45 |
Bud Neck | -5.18 | -7.18 | -8.16 | -2.20 | 0.03 | -7.24 | -12.13 | -6.95 | -6.41 | -3.89 | -8.01 | -13.84 | -10.62 | -7.18 | -0.69 |
Bud Site | -4.25 | 0.02 | -6.23 | -1.83 | -6.38 | -3.07 | -6.79 | -5.30 | -4.86 | -4.04 | -5.01 | -5.90 | -8.57 | -5.90 | -5.84 |
Cell Periphery | -6.58 | -11.54 | -21.98 | -19.44 | -18.15 | 1.74 | 3.75 | -6.23 | -6.59 | -6.94 | -3.40 | -7.04 | -22.16 | -21.41 | -20.73 |
Cytoplasm | 4.53 | 9.96 | 19.18 | 12.43 | 9.64 | -4.50 | 12.66 | 17.46 | 19.07 | 12.37 | -0.83 | 16.06 | 25.73 | 25.30 | 14.24 |
Cytoplasmic Foci | -1.27 | 6.82 | 11.71 | 9.69 | 6.70 | 5.48 | 8.80 | 1.86 | -1.35 | -3.17 | 3.75 | 10.98 | 13.51 | 11.04 | 4.86 |
Eisosomes | -3.35 | -6.47 | -8.32 | -4.15 | -5.81 | -1.52 | -5.10 | -6.03 | -5.74 | -5.13 | -3.80 | -8.23 | -9.17 | -7.26 | -7.29 |
Endoplasmic Reticulum | -1.31 | -0.51 | 3.50 | 3.28 | 3.12 | 5.25 | 8.06 | 8.11 | 3.99 | 1.01 | 4.12 | 7.03 | 7.94 | 3.91 | 1.45 |
Endosome | -3.53 | 0.14 | -0.45 | 2.47 | -0.50 | -0.66 | -1.06 | -1.38 | -1.34 | -1.31 | -3.63 | -0.92 | -0.96 | 0.32 | -0.77 |
Golgi | -5.46 | 5.87 | 14.60 | 16.04 | 13.98 | 2.74 | 14.33 | 3.17 | 1.25 | -4.83 | -1.21 | 14.74 | 18.53 | 17.32 | 7.38 |
Lipid Particles | -7.03 | -10.27 | -15.87 | -10.39 | -11.64 | 0.17 | 0.71 | -6.44 | -6.46 | -6.61 | -4.90 | -6.22 | -16.06 | -13.82 | -14.28 |
Mitochondria | 1.85 | 1.47 | -1.25 | -2.54 | -2.29 | -0.23 | -0.57 | -0.93 | -0.92 | -0.91 | 0.46 | 0.19 | -1.20 | -1.27 | -1.23 |
Mitotic Spindle | -5.06 | -4.00 | 0.35 | 2.79 | 1.90 | -2.71 | -4.01 | -1.82 | 0.22 | 1.84 | -5.05 | -5.35 | 0.19 | 2.14 | 2.56 |
None | -2.44 | 0.67 | -0.85 | 0.65 | -0.96 | -1.35 | 2.55 | 2.83 | 2.75 | 1.02 | -2.23 | 2.64 | -0.76 | 0.62 | -1.40 |
Nuclear Periphery | -4.57 | -11.96 | -7.05 | 0.78 | 8.97 | 2.14 | -6.80 | -5.79 | -6.36 | -2.93 | -2.66 | -13.65 | -9.25 | -5.72 | 6.45 |
Nuclear Periphery Foci | -7.66 | -9.75 | 1.08 | 8.27 | 11.18 | -1.43 | 1.25 | -0.66 | 0.58 | -1.62 | -6.27 | -5.31 | 3.86 | 9.09 | 9.52 |
Nucleolus | -1.17 | -13.45 | -11.21 | 0.68 | -7.35 | -1.33 | -14.78 | -7.14 | -3.28 | -2.15 | -1.39 | -18.37 | -13.22 | -0.02 | -5.34 |
Nucleus | -5.48 | -9.68 | -7.38 | -0.85 | 2.20 | -6.16 | -14.10 | -4.69 | -3.68 | 0.91 | -7.75 | -17.02 | -9.81 | -4.88 | 4.71 |
Peroxisomes | -1.38 | -1.58 | -7.76 | -1.45 | -7.18 | 0.97 | -1.90 | -2.44 | -2.55 | -2.20 | -1.29 | -2.48 | -7.84 | -4.43 | -7.31 |
Vacuole | -9.61 | -23.11 | -34.52 | -27.41 | -14.40 | -1.97 | -30.22 | -19.02 | -18.76 | -10.04 | -8.17 | -37.85 | -38.59 | -35.62 | -15.18 |
Vacuole Periphery | 7.26 | 4.71 | 15.35 | 7.39 | 13.95 | 9.83 | 2.19 | -1.38 | -5.58 | -2.39 | 12.73 | 5.04 | 14.01 | 1.39 | 10.73 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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