Standard name | |
---|---|
Human Ortholog | |
Description | Component of yeast cortical actin cytoskeleton; binds and cross links actin filaments; originally identified by its homology to calponin (contains a calponin-like repeat) but the Scp1p domain structure is more similar to transgelin |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 14 | 47 | 36 | 25 | 25 | 18 | 9 | 23 | 38 | 28 | 39 | 39 | 29 | 46 | 34 | 36 | 37 | 49 | 95 | 118 | 88 | 80 | 125 | 116 |
Bud | 9 | 18 | 16 | 15 | 22 | 19 | 28 | 48 | 35 | 33 | 38 | 34 | 8 | 13 | 17 | 8 | 9 | 13 | 10 | 22 | 10 | 16 | 24 | 33 |
Bud Neck | 0 | 0 | 1 | 1 | 1 | 0 | 1 | 1 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 3 | 0 | 12 | 26 | 7 | 11 | 14 | 12 |
Bud Site | 1 | 4 | 3 | 1 | 2 | 4 | 3 | 6 | 5 | 8 | 3 | 7 | 1 | 1 | 0 | 1 | 2 | 1 | – | – | – | – | – | – |
Cell Periphery | 3 | 7 | 7 | 9 | 12 | 4 | 4 | 8 | 12 | 5 | 3 | 7 | 6 | 6 | 11 | 19 | 22 | 17 | 1 | 3 | 2 | 2 | 4 | 4 |
Cytoplasm | 99 | 215 | 129 | 113 | 109 | 112 | 120 | 157 | 89 | 77 | 74 | 97 | 184 | 249 | 236 | 183 | 211 | 192 | 60 | 119 | 71 | 46 | 78 | 93 |
Endoplasmic Reticulum | 5 | 37 | 16 | 2 | 3 | 12 | 3 | 3 | 21 | 5 | 13 | 9 | 15 | 13 | 16 | 15 | 13 | 12 | 8 | 5 | 3 | 2 | 5 | 7 |
Endosome | 13 | 6 | 1 | 17 | 13 | 14 | 41 | 76 | 25 | 45 | 50 | 44 | 20 | 15 | 20 | 13 | 22 | 12 | 17 | 24 | 7 | 13 | 16 | 23 |
Golgi | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 4 | 2 | 1 | 0 | 5 | 7 | 6 | 2 | 4 | 4 | 1 | 1 | 3 | 6 |
Mitochondria | 20 | 12 | 2 | 5 | 0 | 58 | 32 | 113 | 188 | 140 | 199 | 208 | 0 | 1 | 2 | 8 | 8 | 9 | 34 | 38 | 34 | 21 | 28 | 32 |
Nucleus | 2 | 4 | 0 | 3 | 2 | 1 | 1 | 4 | 3 | 4 | 1 | 5 | 2 | 0 | 0 | 3 | 1 | 2 | 1 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 2 | 2 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 |
Nucleolus | 1 | 0 | 0 | 0 | 1 | 2 | 1 | 6 | 0 | 3 | 0 | 4 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Peroxisomes | 9 | 1 | 1 | 11 | 8 | 8 | 26 | 43 | 20 | 27 | 20 | 27 | 4 | 6 | 18 | 2 | 4 | 1 | 7 | 0 | 2 | 0 | 3 | 4 |
SpindlePole | 5 | 4 | 18 | 18 | 26 | 18 | 33 | 40 | 10 | 31 | 21 | 29 | 7 | 4 | 8 | 13 | 27 | 16 | 4 | 6 | 9 | 10 | 9 | 10 |
Vac/Vac Membrane | 1 | 5 | 1 | 3 | 16 | 3 | 26 | 40 | 15 | 24 | 17 | 17 | 1 | 2 | 5 | 5 | 8 | 3 | 8 | 6 | 0 | 3 | 3 | 1 |
Unique Cell Count | 160 | 314 | 192 | 186 | 198 | 217 | 262 | 440 | 356 | 315 | 373 | 408 | 238 | 324 | 326 | 260 | 309 | 278 | 272 | 382 | 245 | 213 | 322 | 355 |
Labelled Cell Count | 184 | 362 | 232 | 224 | 240 | 273 | 329 | 568 | 466 | 431 | 483 | 529 | 279 | 356 | 372 | 314 | 373 | 329 | 272 | 382 | 245 | 213 | 322 | 355 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.9 | 6.2 | 6.5 | 6.4 | 7.6 | 5.2 | 6.1 | 6.1 | 5.4 | 5.7 | 5.3 | 5.6 | 6.5 | 6.3 | 6.8 | 7.7 | 7.1 | 6.9 | 5.6 | 5.7 | 6.0 |
Std Deviation (1e-4) | 0.9 | 1.2 | 1.2 | 2.2 | 3.5 | 2.3 | 1.8 | 1.8 | 1.4 | 1.4 | 1.2 | 1.3 | 1.4 | 1.3 | 1.5 | 1.7 | 1.2 | 1.4 | 1.6 | 1.4 | 1.9 |
Intensity Change (Log2) | – | – | – | -0.02 | 0.22 | -0.33 | -0.09 | -0.09 | -0.27 | -0.21 | -0.3 | -0.23 | -0.0 | -0.05 | 0.05 | 0.23 | 0.11 | 0.07 | -0.22 | -0.2 | -0.14 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -1.4 | -1.7 | -3.1 | -5.4 | -5.4 | -2.6 | -3.2 | -2.8 | -3.2 | -1.9 | -1.4 | -2.7 | -1.4 | -2.1 | -0.3 |
Bud | -0.1 | 0.9 | 0.2 | 0.8 | 1.0 | 0.6 | 0.8 | 0.7 | 0 | -2.2 | -2.1 | -1.4 | -2.5 | -2.7 | -1.6 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0.6 | 1.1 | -1.1 | 0 | 0 | -0.2 | 0 | 0 | 0 | -0.7 | 0 | -0.2 | 1.7 | 1.6 | 1.2 |
Cytoplasm | -1.3 | -2.5 | -3.2 | -4.5 | -7.3 | -9.6 | -9.5 | -11.1 | -10.2 | 2.3 | 2.4 | 1.3 | 0.7 | 0.3 | 0.4 |
Endoplasmic Reticulum | -3.3 | -3.1 | -1.1 | -3.8 | -5.2 | -1.1 | -3.7 | -2.5 | -3.5 | -0.8 | -2.1 | -1.6 | -1.1 | -1.9 | -1.8 |
Endosome | 3.9 | 3.2 | 3.2 | 5.5 | 5.9 | 3.4 | 5.2 | 5.1 | 4.5 | 3.8 | 2.6 | 3.1 | 2.7 | 3.4 | 2.5 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 7.3 | 4.5 | 7.4 | 12.1 | 10.6 | 12.3 | 12.0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 3.0 | 0 | 0 | 4.2 | 4.2 | 3.0 | 3.8 | 2.9 | 3.3 | 0 | 0 | 2.9 | 0 | 0 | 0 |
SpindlePole | 0.1 | 1.2 | -0.4 | 1.1 | -0.1 | -3.3 | 0.2 | -1.7 | -1.0 | -2.8 | -4.4 | -3.5 | -1.8 | -0.2 | -1.5 |
Vacuole | 0 | 3.7 | 0 | 4.2 | 4.0 | 2.4 | 3.6 | 2.6 | 2.4 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.7112 | 2.0417 | 1.8869 | 1.14 | 1.9658 | 1.9332 | 2.7722 | 2.917 | 2.8925 | 2.8023 | 2.532 | 3.1097 | 5.2607 | 5.5888 | 5.4271 | 5.537 | 5.5233 | 5.5737 |
Actin | 0.2702 | 0.144 | 0.2671 | 0.1949 | 0.2439 | 0.1926 | 0.3251 | 0.1687 | 0.344 | 0.3585 | 0.4059 | 0.278 | 0.3524 | 0.1227 | 0.3254 | 0.3159 | 0.2496 | 0.2218 |
Bud | 0.0033 | 0.01 | 0.0334 | 0.0121 | 0.0155 | 0.0025 | 0.0042 | 0.0128 | 0.039 | 0.0206 | 0.012 | 0.0015 | 0.0046 | 0.0025 | 0.058 | 0.015 | 0.0052 | 0.001 |
Bud Neck | 0.0088 | 0.0048 | 0.0206 | 0.0024 | 0.0006 | 0.1612 | 0.0161 | 0.0049 | 0.0072 | 0.0014 | 0.0075 | 0.1294 | 0.0074 | 0.0032 | 0.0143 | 0.0032 | 0.0009 | 0.1196 |
Bud Periphery | 0.0121 | 0.0124 | 0.0457 | 0.0163 | 0.0665 | 0.0031 | 0.0099 | 0.0134 | 0.0621 | 0.0422 | 0.0328 | 0.0018 | 0.0087 | 0.0027 | 0.0662 | 0.0215 | 0.0048 | 0.0011 |
Bud Site | 0.0306 | 0.1182 | 0.0646 | 0.0055 | 0.0017 | 0.0039 | 0.0527 | 0.1107 | 0.0437 | 0.0055 | 0.0166 | 0.0027 | 0.0616 | 0.0621 | 0.0471 | 0.0029 | 0.0023 | 0.002 |
Cell Periphery | 0.0015 | 0.0023 | 0.0017 | 0.0006 | 0.0008 | 0.0004 | 0.0015 | 0.0016 | 0.0013 | 0.0005 | 0.0014 | 0.0004 | 0.0009 | 0.0016 | 0.0011 | 0.0003 | 0.0003 | 0.0006 |
Cytoplasm | 0.237 | 0.4102 | 0.2471 | 0.2918 | 0.2368 | 0.2637 | 0.1749 | 0.388 | 0.2533 | 0.1677 | 0.1797 | 0.2065 | 0.2293 | 0.4549 | 0.3162 | 0.322 | 0.3494 | 0.373 |
Cytoplasmic Foci | 0.1773 | 0.1029 | 0.106 | 0.1194 | 0.0832 | 0.1843 | 0.1219 | 0.0991 | 0.0633 | 0.1096 | 0.0653 | 0.1258 | 0.0825 | 0.0655 | 0.0522 | 0.0433 | 0.0663 | 0.119 |
Eisosomes | 0.0007 | 0.0004 | 0.0003 | 0.0006 | 0.0005 | 0.0002 | 0.0012 | 0.0002 | 0.0009 | 0.0002 | 0.0006 | 0.0003 | 0.0006 | 0.0005 | 0.0003 | 0.0003 | 0.0004 | 0.0002 |
Endoplasmic Reticulum | 0.0109 | 0.0051 | 0.0069 | 0.0097 | 0.007 | 0.0026 | 0.0068 | 0.0038 | 0.0037 | 0.0087 | 0.0016 | 0.0027 | 0.0069 | 0.012 | 0.003 | 0.0021 | 0.0218 | 0.0057 |
Endosome | 0.0425 | 0.0243 | 0.0447 | 0.0756 | 0.1093 | 0.0229 | 0.0475 | 0.0328 | 0.0178 | 0.0795 | 0.0177 | 0.037 | 0.0213 | 0.0374 | 0.0119 | 0.0501 | 0.0167 | 0.0377 |
Golgi | 0.0399 | 0.0181 | 0.0177 | 0.0454 | 0.0625 | 0.02 | 0.0397 | 0.0225 | 0.0221 | 0.0375 | 0.021 | 0.0265 | 0.0427 | 0.0213 | 0.0195 | 0.0135 | 0.0122 | 0.0298 |
Lipid Particles | 0.0124 | 0.0025 | 0.0024 | 0.0086 | 0.0085 | 0.003 | 0.0104 | 0.0019 | 0.0034 | 0.0064 | 0.0083 | 0.0096 | 0.0036 | 0.0032 | 0.001 | 0.0011 | 0.003 | 0.0056 |
Mitochondria | 0.0037 | 0.0037 | 0.0072 | 0.0173 | 0.0103 | 0.0038 | 0.017 | 0.0075 | 0.0109 | 0.0053 | 0.061 | 0.0095 | 0.0059 | 0.0017 | 0.0069 | 0.0062 | 0.0019 | 0.0027 |
None | 0.0958 | 0.0928 | 0.0816 | 0.1202 | 0.114 | 0.0782 | 0.083 | 0.0789 | 0.0774 | 0.0886 | 0.0457 | 0.0851 | 0.1093 | 0.1937 | 0.0467 | 0.1842 | 0.2085 | 0.0451 |
Nuclear Periphery | 0.0014 | 0.0018 | 0.0021 | 0.0036 | 0.0016 | 0.0004 | 0.0117 | 0.0009 | 0.0019 | 0.0025 | 0.002 | 0.0008 | 0.0012 | 0.0011 | 0.0006 | 0.0004 | 0.0026 | 0.0004 |
Nucleolus | 0.0003 | 0.0005 | 0.0003 | 0.0005 | 0.0001 | 0.0002 | 0.001 | 0.0002 | 0.0004 | 0.0002 | 0.0015 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0 |
Nucleus | 0.0012 | 0.0029 | 0.0016 | 0.0021 | 0.0009 | 0.0012 | 0.002 | 0.0014 | 0.0023 | 0.0087 | 0.0135 | 0.0006 | 0.0006 | 0.0008 | 0.0009 | 0.0005 | 0.0014 | 0.0004 |
Peroxisomes | 0.042 | 0.0367 | 0.0441 | 0.0616 | 0.0301 | 0.0486 | 0.0642 | 0.0455 | 0.04 | 0.0507 | 0.0847 | 0.0792 | 0.0585 | 0.0096 | 0.0249 | 0.0139 | 0.0475 | 0.0323 |
Punctate Nuclear | 0.0026 | 0.0028 | 0.0016 | 0.004 | 0.0015 | 0.005 | 0.0035 | 0.0022 | 0.0032 | 0.0019 | 0.0194 | 0.0015 | 0.001 | 0.0015 | 0.0006 | 0.0005 | 0.0042 | 0.0012 |
Vacuole | 0.005 | 0.0031 | 0.0023 | 0.006 | 0.0038 | 0.0015 | 0.0038 | 0.0025 | 0.0014 | 0.0028 | 0.0014 | 0.0007 | 0.0006 | 0.0015 | 0.0023 | 0.0026 | 0.0006 | 0.0005 |
Vacuole Periphery | 0.0006 | 0.0006 | 0.001 | 0.0019 | 0.001 | 0.0004 | 0.0018 | 0.0006 | 0.0006 | 0.001 | 0.0006 | 0.0004 | 0.0004 | 0.0003 | 0.0007 | 0.0004 | 0.0002 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 25.618 | 24.5367 | 30.3455 | 38.3866 | 17.7937 | 25.6038 | 22.3281 | 29.0237 | 36.1272 | 30.3527 |
Translational Efficiency | 1.699 | 1.3356 | 1.3127 | 1.3718 | 2.5584 | 1.5835 | 1.433 | 1.5825 | 1.4369 | 1.4686 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1871 | 1047 | 169 | 1175 | 1781 | 2733 | 2614 | 1551 | 3652 | 3780 | 2783 | 2726 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 679.69 | 771.76 | 1082.70 | 883.58 | 711.43 | 844.19 | 948.44 | 999.39 | 695.17 | 824.13 | 956.59 | 949.47 |
Standard Deviation | 81.14 | 131.16 | 125.36 | 138.44 | 84.67 | 105.09 | 128.47 | 164.16 | 84.38 | 117.48 | 132.23 | 163.96 |
Intensity Change Log 2 | — | 0.183275 | 0.671685 | 0.378484 | — | 0.246846 | 0.414835 | 0.490326 | — | 0.216136 | 0.546043 | 0.436763 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.010116 | 0.021859 | 0.211020* | 0.218126* | 0.010739 | 0.031500 | 0.152883* | 0.205742* | 0.010420 | 0.028829 | 0.156413* | 0.211080* |
Bud Neck | 0.054239 | 0.028274 | 0.018149 | 0.019705 | 0.043086 | 0.060616 | 0.038916 | 0.018091 | 0.048800 | 0.051657 | 0.037655 | 0.018786 |
Bud Site | 0.037627 | 0.068476 | 0.210413* | 0.269952* | 0.035375 | 0.092790 | 0.278199* | 0.222627* | 0.036529 | 0.086055 | 0.274082* | 0.243026* |
Cell Periphery | 0.000351 | 0.000498 | 0.001194 | 0.001141 | 0.000444 | 0.000469 | 0.000447 | 0.000913 | 0.000396 | 0.000477 | 0.000492 | 0.001011 |
Cytoplasm | 0.270134* | 0.292988* | 0.109681* | 0.177443* | 0.346902* | 0.357044* | 0.246050* | 0.231677* | 0.307572* | 0.339301* | 0.237768* | 0.208300* |
Cytoplasmic Foci | 0.348595* | 0.327028* | 0.102159 | 0.069226 | 0.330665* | 0.202255* | 0.053596 | 0.063955 | 0.339851* | 0.236815* | 0.056545 | 0.066227 |
Eisosomes | 0.001687 | 0.001133 | 0.000656 | 0.001127 | 0.001775 | 0.001472 | 0.000418 | 0.001008 | 0.001730 | 0.001378 | 0.000432 | 0.001060 |
Endoplasmic Reticulum | 0.001506 | 0.003016 | 0.014811 | 0.004467 | 0.001460 | 0.001397 | 0.001479 | 0.001873 | 0.001483 | 0.001846 | 0.002289 | 0.002991 |
Endosome | 0.023163 | 0.043923 | 0.020708 | 0.008936 | 0.018049 | 0.016422 | 0.006531 | 0.016147 | 0.020669 | 0.024039 | 0.007392 | 0.013039 |
Golgi | 0.033210 | 0.034037 | 0.005122 | 0.005250 | 0.020430 | 0.022982 | 0.005037 | 0.011212 | 0.026977 | 0.026044 | 0.005042 | 0.008642 |
Lipid Particles | 0.025845 | 0.017482 | 0.031140 | 0.005442 | 0.020225 | 0.008650 | 0.003006 | 0.007224 | 0.023105 | 0.011096 | 0.004715 | 0.006456 |
Mitochondria | 0.029046 | 0.014631 | 0.000292 | 0.001737 | 0.013416 | 0.019034 | 0.003810 | 0.003140 | 0.021424 | 0.017815 | 0.003596 | 0.002535 |
Mitotic Spindle | 0.003432 | 0.004471 | 0.014519 | 0.010440 | 0.002402 | 0.008997 | 0.014887 | 0.021832 | 0.002930 | 0.007743 | 0.014864 | 0.016922 |
None | 0.002709 | 0.003457 | 0.000113 | 0.002150 | 0.001541 | 0.001077 | 0.000392 | 0.000369 | 0.002139 | 0.001736 | 0.000375 | 0.001137 |
Nuclear Periphery | 0.000139 | 0.000239 | 0.000281 | 0.000125 | 0.000151 | 0.000110 | 0.000110 | 0.000131 | 0.000145 | 0.000146 | 0.000121 | 0.000129 |
Nuclear Periphery Foci | 0.000236 | 0.000921 | 0.007448 | 0.001192 | 0.000258 | 0.000148 | 0.000415 | 0.000638 | 0.000247 | 0.000362 | 0.000842 | 0.000877 |
Nucleolus | 0.000636 | 0.001548 | 0.000114 | 0.000156 | 0.000700 | 0.000417 | 0.000132 | 0.000127 | 0.000668 | 0.000731 | 0.000131 | 0.000139 |
Nucleus | 0.054426 | 0.030808 | 0.023319 | 0.028290 | 0.058725 | 0.041171 | 0.022007 | 0.023638 | 0.056523 | 0.038301 | 0.022087 | 0.025643 |
Peroxisomes | 0.066925 | 0.053561 | 0.089555 | 0.086708 | 0.053766 | 0.091267 | 0.117988* | 0.082786 | 0.060508 | 0.080823 | 0.116262* | 0.084476 |
Vacuole | 0.034271 | 0.049079 | 0.139011 | 0.087955 | 0.038933 | 0.040732 | 0.053508 | 0.084936 | 0.036544 | 0.043044 | 0.058701 | 0.086238 |
Vacuole Periphery | 0.001706 | 0.002571 | 0.000295 | 0.000431 | 0.000958 | 0.001450 | 0.000189 | 0.001934 | 0.001341 | 0.001761 | 0.000195 | 0.001287 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -4.31 | -8.46 | -22.07 | -20.00* | -0.98 | -9.11 | -26.27* | -21.48 | -18.25 | -3.68 | -10.61 | -28.13 | -30.72* | -26.60* | -5.90 |
Bud Neck | 7.81 | 11.19 | 11.82 | 3.59 | -0.72 | -5.61 | 2.20 | 10.24 | 18.00 | 10.71 | -1.17 | 5.79 | 15.81 | 17.78 | 10.78 |
Bud Site | -6.39 | -8.54 | -25.21 | -19.90 | -3.47 | -15.06 | -36.83* | -22.48 | -13.40 | 7.30 | -16.64 | -38.75 | -33.45 | -22.63 | 4.35 |
Cell Periphery | -2.41 | -7.59 | -10.20 | -6.93 | 0.57 | -0.32 | 0.39 | -3.95 | -3.74 | -5.50 | -1.52 | -1.95 | -9.28 | -7.00 | -9.04 |
Cytoplasm | -2.16 | 9.96 | 9.70 | 9.74 | -4.32 | -1.30 | 9.87 | 9.60 | 11.31 | 1.04 | -5.11 | 7.63 | 12.49 | 16.61 | 4.34 |
Cytoplasmic Foci | 2.44 | 26.57 | 44.58 | 32.57 | 3.21 | 19.40 | 48.20 | 43.78 | 29.00 | -2.86 | 19.99 | 67.27 | 62.87 | 41.10 | -3.36 |
Eisosomes | 2.40 | 8.57 | 2.79 | 0.27 | -1.98 | 1.54 | 7.83 | 4.28 | 3.96 | -3.49 | 2.64 | 12.72 | 5.22 | 3.06 | -4.48 |
Endoplasmic Reticulum | -2.79 | -7.45 | -7.24 | -2.58 | 5.87 | 0.20 | -1.40 | -1.54 | -1.65 | -0.48 | -1.48 | -5.41 | -6.44 | -4.17 | -1.30 |
Endosome | -6.80 | 1.29 | 9.62 | 12.09 | 4.87 | 1.37 | 11.17 | 2.79 | 1.88 | -5.40 | -2.72 | 14.79 | 8.07 | 9.96 | -4.73 |
Golgi | -0.08 | 17.02 | 16.39 | 12.23 | -0.42 | -1.45 | 11.74 | 6.48 | 8.44 | -3.33 | 0.79 | 20.57 | 15.47 | 14.61 | -2.99 |
Lipid Particles | 3.46 | -0.60 | 11.57 | 6.58 | 3.81 | 7.20 | 11.49 | 7.68 | 1.62 | -3.43 | 9.26 | 15.32 | 13.39 | 5.30 | -1.76 |
Mitochondria | 5.97 | 16.59 | 15.75 | 7.30 | -6.18 | -3.43 | 7.78 | 7.70 | 12.94 | 1.26 | 2.60 | 16.21 | 16.78 | 15.62 | 2.44 |
Mitotic Spindle | -0.91 | -2.35 | -3.24 | -2.64 | 1.00 | -4.90 | -8.01 | -7.71 | -4.98 | -2.51 | -4.79 | -8.44 | -8.23 | -5.12 | -0.80 |
None | -0.55 | 3.87 | 0.64 | 0.97 | -1.77 | 1.35 | 3.35 | 3.34 | 3.91 | 0.06 | 0.83 | 4.56 | 1.81 | 1.12 | -1.51 |
Nuclear Periphery | -2.24 | -4.69 | -1.61 | 1.44 | 4.05 | 2.09 | -2.15 | -3.65 | -6.05 | -1.81 | -0.28 | -3.74 | -3.90 | -2.99 | 0.00 |
Nuclear Periphery Foci | -4.95 | -6.66 | -6.74 | -1.52 | 5.74 | 3.95 | -3.47 | -3.55 | -4.22 | -1.11 | -2.85 | -6.57 | -6.84 | -5.43 | 0.31 |
Nucleolus | -1.48 | 3.13 | 2.92 | 2.21 | -0.37 | 1.36 | 2.61 | 2.70 | 4.62 | 0.84 | -0.45 | 3.91 | 3.98 | 3.08 | 0.52 |
Nucleus | 7.33 | 1.14 | 5.60 | -1.19 | 0.92 | 6.28 | 10.88 | 9.02 | 3.44 | -0.51 | 8.48 | 11.61 | 10.29 | 2.43 | -0.92 |
Peroxisomes | 3.36 | -0.69 | -2.37 | -4.97 | -0.45 | -8.92 | -11.94 | -3.76 | 4.04 | 6.82 | -6.37 | -11.43 | -4.10 | 1.59 | 6.80 |
Vacuole | -5.14 | -8.57 | -14.73 | -11.05 | 3.40 | -0.87 | -13.07 | -17.89 | -17.71 | -9.24 | -4.12 | -16.48 | -23.29 | -21.23 | -8.69 |
Vacuole Periphery | -3.02 | 9.87 | 8.20 | 8.40 | -1.04 | -3.97 | 11.89 | -1.47 | -0.18 | -3.54 | -3.20 | 14.55 | 1.32 | 2.96 | -3.87 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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