Standard name
Human Ortholog
Description Rab escort protein; forms a complex with the Ras-like small GTPase Ypt1p that is required for the prenylation of Ypt1p by protein geranylgeranyltransferase type II (Bet2p-Bet4p); sequence similarity to mammalian choroideraemia gene; relative distribution to the nucleus increases upon DNA replication stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.94 0.97 0.91 0.91 0.82 0.85 0.75 0.7 0.62 0.63 0.61 0.61 0.84 0.89 0.89 0.88 0.82 0.84 0.8 0.87 0.65 0.76 0.75
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.11 0.17 0.13 0.32 0.33 0.32 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0.05 0.05 0 0.05 0 0.06 0.11 0.19 0.06 0.09 0.13 0.06 0.19 0.08 0.09
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.11 0 0.12 0.2 0.27 0.09 0.17 0.21 0.14 0.07 0.13 0.48 0.25 0.11 0.13 0.11 0.27 0 0 0 0.08 0.07 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 2 0 0 0 0 1
Bud 0 0 1 0 0 1 0 7 1 6 2 0 0 0 0 0 0 5 2 0 1 5 4
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 2 2 2 5 4 7 5 0 0 0 0 1 0 0 0 0 0 0 0
Cytoplasm 342 217 319 215 235 305 357 402 269 254 268 51 155 173 237 153 148 315 178 308 135 258 282
Endoplasmic Reticulum 0 4 3 0 1 2 4 4 9 6 12 0 4 1 9 5 4 1 1 4 3 7 7
Endosome 0 0 1 0 0 0 0 2 0 0 1 1 0 0 0 1 0 3 1 1 3 3 6
Golgi 3 0 0 1 0 0 0 0 0 0 0 0 0 0 2 0 2 1 0 0 1 0 1
Mitochondria 0 0 4 2 9 40 83 73 140 134 139 0 0 0 4 5 1 1 0 2 1 2 2
Nucleus 12 0 0 1 1 6 10 15 20 19 19 4 7 11 29 33 11 32 29 20 40 29 33
Nuclear Periphery 0 0 0 0 0 0 0 1 2 2 2 0 0 0 0 0 0 1 0 2 1 3 1
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 0 2
Vac/Vac Membrane 40 4 42 47 78 33 79 119 61 27 57 40 46 21 35 20 49 6 5 7 16 24 23
Unique Cell Count 363 223 349 237 285 360 475 572 432 404 440 83 184 195 266 174 181 375 224 357 209 342 377
Labelled Cell Count 397 225 370 266 326 389 535 628 506 456 505 96 212 206 316 218 215 375 224 357 209 342 377


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 13.7 13.6 10.3 9.9 8.9 7.5 7.0 7.3 6.3 6.5 6.6 12.2 12.6 12.2 9.1 9.4 12.5 13.4 13.6 14.0
Std Deviation (1e-4) 1.7 2.0 1.6 1.6 1.7 1.3 1.3 1.4 1.2 1.2 1.3 1.9 2.1 1.8 2.0 2.1 2.2 2.1 1.9 2.3
Intensity Change (Log2) -0.06 -0.21 -0.46 -0.56 -0.49 -0.72 -0.65 -0.65 0.25 0.29 0.25 -0.17 -0.13 0.28 0.38 0.41 0.45


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.3 -3.4 -2.7 -6.0 -7.5 -9.4 -9.2 -9.7 -7.0 -2.5 -1.0 -1.0 -1.3 -3.3
Endoplasmic Reticulum 0 0 0 0 0 1.4 0 1.9 0 0 0 2.2 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 1.8 5.5 7.5 6.2 11.2 11.3 11.0 0 0 0 0 0 0
Nucleus 0 0 0 0 3.1 4.1 4.1 3.9 0 0 0 6.3 8.4 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 2.6 4.9 -1.2 1.8 3.4 0.9 -2.5 0.4 7.6 3.8 -0.4 0.4 -0.2 4.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 9.3678 11.2771 10.1223 9.4798 10.0291 10.4353 11.6382 14.0926 12.8255 12.7158 11.4944 12.8791 11.9623 14.6515 13.1197 12.9748 12.3346 13.4434
Actin 0.0198 0.0004 0.003 0.0035 0.0004 0.0014 0.0217 0.0005 0.0022 0.0008 0.0097 0.0002 0.0135 0.001 0.0095 0.002 0.0182 0.0018
Bud 0.0008 0.0001 0.0006 0.0007 0.0002 0.0004 0.001 0.0001 0.0004 0 0.0023 0.0001 0.001 0.0001 0.0002 0.0004 0.0008 0.0002
Bud Neck 0.0045 0.0004 0.0033 0.0022 0.0012 0.0024 0.001 0.0004 0.0008 0.0004 0.0008 0.0016 0.0009 0.0003 0.0006 0.001 0.0013 0.0075
Bud Periphery 0.0008 0 0.0006 0.0005 0 0.0002 0.0009 0 0.0002 0 0.0023 0 0.0021 0 0.0001 0.0003 0.0013 0.0001
Bud Site 0.0038 0.0004 0.0025 0.0008 0.0001 0.0002 0.0035 0.0003 0.0052 0.0002 0.0014 0.0001 0.0022 0.0002 0.0006 0.0008 0.0013 0.0001
Cell Periphery 0.0002 0 0.0002 0 0 0 0.0001 0 0 0 0.0001 0 0.0002 0 0 0 0.0002 0
Cytoplasm 0.5029 0.7732 0.6623 0.5571 0.7985 0.5616 0.5731 0.7608 0.7166 0.5644 0.6721 0.5975 0.6868 0.7853 0.748 0.7741 0.5956 0.5736
Cytoplasmic Foci 0.0161 0.0014 0.0126 0.0056 0.0052 0.0024 0.0216 0.0008 0.0112 0.0754 0.0172 0.0012 0.0161 0.0032 0.007 0.0176 0.0091 0.0026
Eisosomes 0.0002 0 0 0 0 0 0.0001 0 0 0 0 0 0.0001 0 0.0001 0 0.0001 0
Endoplasmic Reticulum 0.0094 0.0015 0.006 0.0026 0.0032 0.003 0.0029 0.0009 0.0012 0.0006 0.001 0.0014 0.0058 0.0008 0.0014 0.0014 0.0032 0.0025
Endosome 0.0321 0.0007 0.0133 0.0057 0.0085 0.0033 0.0125 0.0003 0.0021 0.0011 0.0122 0.0007 0.0138 0.0002 0.0027 0.0111 0.0131 0.0041
Golgi 0.0074 0.0001 0.0026 0.0006 0.0001 0.0002 0.008 0 0.0007 0.0008 0.0077 0 0.0034 0.0001 0.0031 0.0046 0.0055 0.0012
Lipid Particles 0.0095 0 0.0011 0.0004 0 0.0001 0.0038 0 0.0012 0.0027 0.0049 0 0.0056 0 0.004 0.0036 0.0135 0.0008
Mitochondria 0.0044 0.0002 0.0057 0.0022 0.0003 0.0015 0.0089 0.0002 0.0007 0.0001 0.0266 0.0002 0.0078 0.0002 0.0017 0.0017 0.0046 0.0011
None 0.007 0.0011 0.0018 0.0021 0.0017 0.0011 0.0102 0.0011 0.0011 0.0006 0.0008 0.0007 0.0049 0.0005 0.0027 0.0009 0.0014 0.0009
Nuclear Periphery 0.035 0.0097 0.0159 0.0173 0.0117 0.025 0.0204 0.0055 0.0085 0.0055 0.0056 0.0097 0.0135 0.0069 0.0067 0.0072 0.0149 0.0111
Nucleolus 0.0011 0.0002 0.0004 0.0013 0.0001 0.0005 0.0028 0.0001 0.0002 0.0001 0.0001 0.0001 0.0004 0 0.0001 0.0001 0.0005 0.0002
Nucleus 0.3183 0.2078 0.2561 0.3762 0.1625 0.3897 0.2758 0.227 0.2427 0.3372 0.1984 0.3839 0.2032 0.1982 0.2013 0.1625 0.2821 0.3881
Peroxisomes 0.0092 0 0.0023 0.0006 0.0001 0.0003 0.0145 0.0001 0.001 0.0075 0.0338 0.0001 0.0071 0.0011 0.0086 0.0068 0.0224 0.0012
Punctate Nuclear 0.0127 0.0018 0.0049 0.0191 0.0042 0.0046 0.0154 0.0017 0.0034 0.0023 0.001 0.0021 0.0086 0.0016 0.0014 0.0031 0.0047 0.0021
Vacuole 0.0033 0.0008 0.0037 0.0011 0.0016 0.0015 0.0013 0.0002 0.0004 0.0001 0.0013 0.0005 0.0021 0.0002 0.0003 0.0006 0.0047 0.0008
Vacuole Periphery 0.0014 0.0001 0.001 0.0004 0.0004 0.0006 0.0006 0 0.0002 0 0.0006 0.0001 0.001 0 0.0001 0.0002 0.0014 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 60.8943 55.5245 52.5118 68.0843 58.4246 44.4282 58.3423 54.7937 57.4946 67.3712
Translational Efficiency 0.7061 0.6042 0.5797 0.4522 0.607 0.8066 0.5449 0.4751 0.4916 0.5338

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
115 375 149 1172 97 1744 2579 923 212 2119 2728 2095

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1212.44 1086.56 1480.80 1195.24 1118.78 1155.39 1274.00 1200.86 1169.59 1143.21 1285.30 1197.72
Standard Deviation 144.14 223.37 199.15 174.29 153.58 182.52 166.58 187.48 155.69 192.19 174.95 180.24
Intensity Change Log 2 -0.158146 0.288463 -0.020613 0.046454 0.187439 0.102142 -0.056331 0.240862 0.039603

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.004026 0.000411 0.002294 0.018308 0.001004 0.000239 0.000153 0.003765 0.002643 0.000270 0.000270 0.011901
Bud Neck 0.004222 0.001027 0.004768 0.019881 0.001396 0.001492 0.003948 0.014112 0.002929 0.001410 0.003993 0.017339
Bud Site 0.099312 0.002115 0.015499 0.062005 0.010401 0.002577 0.003306 0.029335 0.058631 0.002495 0.003972 0.047611
Cell Periphery 0.000416 0.000128 0.000487 0.000308 0.000335 0.000095 0.000058 0.000147 0.000379 0.000101 0.000082 0.000237
Cytoplasm 0.287503* 0.614960* 0.353221* 0.315545* 0.662263* 0.589853* 0.479242* 0.512242* 0.458974* 0.594296* 0.472359* 0.402204*
Cytoplasmic Foci 0.096889 0.007979 0.011409 0.028375 0.049900 0.017621 0.001734 0.026481 0.075389 0.015915 0.002262 0.027540
Eisosomes 0.000076 0.000012 0.000024 0.000051 0.000045 0.000016 0.000005 0.000039 0.000062 0.000015 0.000006 0.000045
Endoplasmic Reticulum 0.002603 0.002534 0.003990 0.002758 0.004321 0.002006 0.000596 0.001873 0.003389 0.002099 0.000782 0.002368
Endosome 0.043640 0.000791 0.002997 0.027671 0.003403 0.002840 0.000264 0.027887 0.025230 0.002478 0.000413 0.027766
Golgi 0.060403 0.000130 0.000944 0.031917 0.000612 0.001285 0.000079 0.018093 0.033046 0.001081 0.000126 0.025826
Lipid Particles 0.002549 0.000480 0.002174 0.002332 0.002499 0.000795 0.000299 0.003645 0.002526 0.000739 0.000401 0.002910
Mitochondria 0.003624 0.000203 0.000728 0.015597 0.000302 0.002227 0.000685 0.007450 0.002104 0.001869 0.000688 0.012008
Mitotic Spindle 0.029498 0.001179 0.002247 0.147101* 0.031446 0.006554 0.005407 0.055603 0.030390 0.005603 0.005234 0.106789*
None 0.000336 0.014862 0.003346 0.014005 0.001623 0.007760 0.008883 0.011485 0.000925 0.009017 0.008580 0.012894
Nuclear Periphery 0.000512 0.000436 0.001042 0.001803 0.000381 0.000524 0.000767 0.001139 0.000452 0.000508 0.000782 0.001510
Nuclear Periphery Foci 0.006847 0.004031 0.006722 0.002578 0.007072 0.002850 0.000460 0.002403 0.006950 0.003059 0.000802 0.002501
Nucleolus 0.004428 0.000348 0.000195 0.001110 0.000345 0.001012 0.000146 0.000702 0.002560 0.000894 0.000148 0.000930
Nucleus 0.021095 0.316540* 0.514454* 0.184703* 0.149206* 0.326267* 0.477556* 0.185757* 0.079712 0.324545* 0.479571* 0.185167*
Peroxisomes 0.002796 0.000084 0.000478 0.003990 0.000704 0.000731 0.000098 0.003563 0.001839 0.000617 0.000119 0.003802
Vacuole 0.325561* 0.031650 0.072759 0.095857 0.072425 0.032592 0.016236 0.085570 0.209739* 0.032425 0.019324 0.091325
Vacuole Periphery 0.003663 0.000099 0.000221 0.024108 0.000319 0.000666 0.000079 0.008712 0.002133 0.000565 0.000087 0.017325

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 2.29 -2.07 -1.56 -2.67 -0.62 3.73 3.77 -0.35 -1.55 -1.56 3.77 3.44 -1.59 -3.20 -3.08
Bud Neck 1.57 -6.07 -5.22 -6.18 -0.02 0.20 -4.59 -3.94 -4.08 -1.54 1.55 -4.46 -6.56 -7.43 -3.42
Bud Site 2.67 1.85 1.36 -7.54 -1.70 3.59 3.40 -0.14 -5.28 -4.97 3.15 3.03 1.29 -9.25 -8.41
Cell Periphery 1.55 -6.56 0.23 -2.79 7.53 3.99 4.30 3.50 -3.16 -6.73 4.54 4.24 2.79 -6.14 -5.27
Cytoplasm -8.81 0.54 -0.12 16.23 -0.99 2.06 7.88 7.47 14.39 -0.37 -5.09 2.39 4.38 26.16 6.28
Cytoplasmic Foci 5.62 5.09 5.72 0.46 2.33 4.25 6.37 5.36 5.27 -6.92 6.37 9.07 7.86 6.15 -8.87
Eisosomes 6.88 3.00 3.05 -8.03 -0.44 3.90 5.03 2.30 -4.73 -8.90 7.18 8.89 3.65 -9.08 -15.51
Endoplasmic Reticulum -2.31 -4.70 -1.25 1.76 4.48 2.63 4.07 3.53 4.37 -3.96 1.45 4.04 2.64 3.21 -5.97
Endosome 3.07 2.61 2.30 -4.23 -1.41 1.15 4.96 -0.54 -1.40 -3.83 2.90 3.68 2.11 -2.68 -6.07
Golgi 1.97 1.90 1.48 -3.60 -3.02 -1.37 3.45 -1.94 -1.25 -2.46 1.82 2.01 1.28 -2.99 -4.36
Lipid Particles 4.31 -3.11 -0.69 -10.50 3.12 2.23 2.45 0.80 -3.61 -4.34 3.71 3.65 0.40 -7.93 -8.37
Mitochondria 1.26 0.35 -1.66 -3.52 -2.63 -2.60 -7.01 -3.38 0.61 -1.87 -1.43 -0.31 -2.59 -0.81 -3.16
Mitotic Spindle 1.08 0.96 -4.16 -9.84 -9.53 1.34 1.36 0.01 -5.51 -5.88 1.54 1.53 -2.43 -10.46 -10.77
None -6.31 -5.58 -12.05 1.77 -8.17 -6.53 -24.39* -12.41 -2.42 -1.35 -8.99 -30.17* -17.08 -1.25 -1.35
Nuclear Periphery -1.11 -7.57 -16.07 -16.27 -5.55 -2.20 -12.94 -7.87 -7.44 -2.56 -2.14 -15.81 -16.52 -16.83 -8.00
Nuclear Periphery Foci 0.99 -1.74 3.94 4.21 4.82 3.36 5.56 3.91 1.53 -4.87 3.67 7.24 5.48 3.63 -5.82
Nucleolus 0.83 0.90 0.69 -1.33 -2.19 -2.17 1.15 -1.06 1.41 -2.01 0.04 1.02 0.55 1.36 -3.25
Nucleus -22.15 -20.85 -29.74 4.60 9.91 -9.65 -19.65 -5.30 8.11 29.08 -22.78 -40.63 -16.53 9.62 36.33
Peroxisomes 2.69 1.96 0.96 -3.19 -1.66 -0.02 3.31 0.54 0.60 -2.92 1.26 3.79 1.09 -0.26 -4.30
Vacuole 15.46 11.23 7.08 -23.67 -8.83 3.81 3.45 -8.26 -22.24 -21.83 12.51 12.11 2.08 -35.17 -33.95
Vacuole Periphery 1.78 1.53 -1.70 -2.87 -2.73 -1.62 3.75 -1.66 -1.05 -2.09 0.69 2.14 -2.18 -2.72 -3.44
Standard name
Human Ortholog
Description Rab escort protein; forms a complex with the Ras-like small GTPase Ypt1p that is required for the prenylation of Ypt1p by protein geranylgeranyltransferase type II (Bet2p-Bet4p); sequence similarity to mammalian choroideraemia gene; relative distribution to the nucleus increases upon DNA replication stress
Localization
Cell Percentages cytoplasm (82%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Mrs6

Mrs6


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mrs6-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available