Standard name
Human Ortholog
Description Alcohol acetyltransferase; responsible for the major part of volatile acetate ester production during fermentation; main enzyme involved in terpenyl acetate synthesis; potential roles in lipid and sterol metabolism
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.05 0 0 0 0 0 0.05
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0.16 0 0 0 0 0 0
Cytoplasm 0.52 0.74 0.88 0.76 0.81 0.64 0.66 0.68 0.64 0.55 0.56 0.49 0.92 0.93 0.95 0.5 0.5 0.47 0.21 0.55 0.44 0.46 0.33 0.28
Endoplasmic Reticulum 0.31 0.19 0 0.05 0 0.05 0 0 0 0 0 0 0.06 0 0 0 0.05 0 0.55 0.24 0.14 0.33 0.35 0.27
Endosome 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0.12 0.18 0.16 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.19 0.12 0.13 0.22 0.13 0.47 0.47 0.42 0.59 0.63 0.73 0.71 0 0 0 0.1 0.09 0.16 0.11 0.07 0.23 0.07 0.14 0.14
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.06 0 0 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0.27 0.23 0.18 0 0.09 0.05 0 0 0.08
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 0 0 0 0 1 0 0 0 0 0 0 0 0 0 10 2 2 2 0 4 3 13 22
Bud 3 1 6 2 0 6 7 14 9 3 3 5 3 3 3 0 0 0 0 0 3 6 16 29
Bud Neck 0 0 1 0 0 0 0 2 3 0 2 0 0 0 0 0 0 0 2 1 2 2 5 8
Bud Site 0 0 0 0 0 0 1 2 3 2 1 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 1 1 0 0 2 4 2 1 2 1 2 4 8 2 14 1 6 0 0 1 2 8 11
Cytoplasm 56 75 159 81 159 174 213 325 219 178 99 122 228 461 336 115 11 18 29 64 85 109 154 116
Endoplasmic Reticulum 34 19 1 5 4 15 1 1 4 1 1 2 14 17 4 9 1 0 76 28 27 78 163 110
Endosome 0 0 0 0 9 1 4 4 0 0 1 0 1 7 5 29 4 6 2 0 4 1 6 3
Golgi 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 0 2 3 2
Mitochondria 21 12 23 23 26 127 152 201 202 204 130 176 0 3 4 23 2 6 15 8 45 16 65 58
Nucleus 0 0 1 0 0 1 0 2 2 1 0 0 0 0 0 5 0 0 0 0 0 0 0 0
Nuclear Periphery 2 0 1 0 0 0 0 2 0 1 0 1 0 0 0 1 0 0 0 0 0 0 2 0
Nucleolus 0 0 0 1 1 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0
SpindlePole 0 0 1 1 2 2 3 6 7 1 2 7 0 1 1 2 0 1 0 0 2 1 4 4
Vac/Vac Membrane 6 2 4 5 10 10 10 16 7 6 2 3 7 9 7 62 5 7 2 10 9 7 13 33
Unique Cell Count 108 102 180 106 196 273 321 476 344 322 178 249 249 494 352 232 22 38 138 117 194 239 468 414
Labelled Cell Count 125 110 198 119 212 339 396 577 457 399 242 319 257 510 363 273 26 46 138 117 194 239 468 414


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.1 4.7 4.2 4.1 4.3 3.4 3.1 3.4 3.0 2.7 2.8 2.5 5.3 5.1 5.1 7.9 8.3 9.9 5.5 5.3 6.0
Std Deviation (1e-4) 1.2 1.2 1.3 1.3 1.6 1.0 1.2 1.4 1.0 0.8 1.2 0.8 1.3 1.5 1.8 1.3 1.4 1.4 1.7 1.6 2.3
Intensity Change (Log2) – – – -0.04 0.05 -0.3 -0.43 -0.3 -0.48 -0.63 -0.6 -0.72 0.34 0.27 0.28 0.92 0.99 1.24 0.38 0.35 0.52


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 – –
Bud Neck – – – – – – – – – – – – 0 – –
Bud Site – – – – – – – – – – – – 0 – –
Cell Periphery – – – – – – – – – – – – 0 – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 – –
Endosome – – – – – – – – – – – – 0 – –
Golgi – – – – – – – – – – – – 0 – –
Mitochondria – – – – – – – – – – – – 0 – –
Nuclear Periphery – – – – – – – – – – – – 0 – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 – –
Peroxisomes – – – – – – – – – – – – 0 – –
SpindlePole – – – – – – – – – – – – 0 – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 – –
Cytoplasm – – – – – – – – – – – – 0 – –
Nucleus – – – – – – – – – – – – 0 – –
Vacuole – – – – – – – – – – – – 6.7 – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.533 1.385 0.9932 0.988 0.496 1.1416 1.1691 1.3507 1.3374 1.0089 1.3286 1.2247 2.3224 2.3233 1.8454 2.2355 2.0331 1.8122
Actin 0.0631 0.0037 0.0077 0.0009 0.0018 0.0044 0.0674 0.0021 0.0173 0.0018 0.0033 0.0131 0.0574 0.0008 0.0086 0.0145 0.0759 0.0009
Bud 0.0008 0.0002 0.0003 0.0001 0.0007 0.0031 0.0008 0.0002 0.0002 0.0001 0 0.0001 0.003 0.0016 0.0006 0.0005 0.0001 0.0001
Bud Neck 0.004 0.0003 0.0009 0.0001 0.0014 0.0014 0.002 0.0003 0.0007 0.0009 0.0009 0.0012 0.0012 0.0003 0.0007 0.0006 0.0004 0.0014
Bud Periphery 0.002 0.0003 0.0005 0.0002 0.0038 0.0077 0.0013 0.0002 0.0003 0.0003 0 0.0002 0.0048 0.001 0.0009 0.0005 0.0001 0.0003
Bud Site 0.01 0.0058 0.001 0.0001 0.0022 0.0008 0.0063 0.0122 0.0022 0.0002 0.0001 0.0003 0.0057 0.0256 0.0199 0.0026 0.0006 0.0001
Cell Periphery 0.0059 0.002 0.0008 0.0003 0.0009 0.001 0.0013 0.0022 0.0012 0.0016 0.0003 0.0006 0.0036 0.0028 0.0022 0.0011 0.0002 0.0008
Cytoplasm 0.1132 0.4506 0.2404 0.357 0.2743 0.3806 0.1902 0.3987 0.3821 0.4138 0.7828 0.3264 0.2032 0.3595 0.4537 0.2743 0.2747 0.4441
Cytoplasmic Foci 0.0323 0.0097 0.0124 0.0152 0.0163 0.0116 0.0492 0.0189 0.0209 0.0163 0.0103 0.0301 0.0522 0.0064 0.0089 0.0044 0.0053 0.0176
Eisosomes 0.0007 0.0002 0.0005 0.0002 0.0004 0.0002 0.0009 0.0004 0.0005 0.0003 0 0.0001 0.0006 0.0002 0.0004 0.0004 0.0001 0.0001
Endoplasmic Reticulum 0.0724 0.1182 0.0687 0.0244 0.0339 0.0867 0.2204 0.1712 0.1564 0.2233 0.132 0.214 0.1938 0.2031 0.1654 0.1076 0.1653 0.2959
Endosome 0.0214 0.0134 0.0118 0.0205 0.0355 0.0067 0.0847 0.0203 0.032 0.0195 0.0162 0.0402 0.0236 0.0072 0.014 0.0129 0.0244 0.0228
Golgi 0.0191 0.0015 0.0026 0.0016 0.0026 0.0026 0.0244 0.0023 0.0164 0.0016 0.0011 0.0106 0.027 0.0008 0.0049 0.0059 0.0068 0.0039
Lipid Particles 0.0101 0.0026 0.0067 0.0019 0.0083 0.0043 0.0713 0.0105 0.0067 0.0403 0.0021 0.0116 0.0271 0.0033 0.0086 0.0026 0.0017 0.0084
Mitochondria 0.0109 0.0396 0.0021 0.0011 0.0287 0.0043 0.0176 0.0007 0.0067 0.0016 0.0002 0.0023 0.0121 0.0004 0.0118 0.0224 0.0008 0.0094
None 0.5982 0.3311 0.6237 0.5663 0.5561 0.4743 0.2117 0.343 0.3318 0.2477 0.0423 0.3108 0.3384 0.3683 0.2866 0.5104 0.4396 0.1806
Nuclear Periphery 0.0041 0.0087 0.0067 0.0027 0.0025 0.0026 0.0178 0.005 0.0046 0.0151 0.0031 0.0053 0.0067 0.0094 0.0034 0.0044 0.0018 0.0048
Nucleolus 0.0006 0.0003 0.0004 0.0001 0.0031 0.0001 0.0007 0.0002 0.0003 0.0004 0 0.0001 0.0005 0.0004 0.0004 0.0001 0 0.0001
Nucleus 0.0014 0.0038 0.005 0.0009 0.0022 0.0016 0.0024 0.0017 0.0019 0.0023 0.0014 0.0013 0.0025 0.0024 0.0016 0.0015 0.0005 0.002
Peroxisomes 0.0198 0.0004 0.0017 0.0011 0.0165 0.0026 0.0118 0.0003 0.009 0.0063 0.0001 0.0236 0.0233 0.0001 0.0011 0.0076 0.0001 0.0002
Punctate Nuclear 0.0039 0.0024 0.0013 0.0006 0.0008 0.0005 0.0027 0.001 0.0023 0.0009 0.0009 0.0009 0.0077 0.0011 0.001 0.0002 0.0002 0.0012
Vacuole 0.0049 0.0048 0.0041 0.0041 0.0069 0.0026 0.0118 0.008 0.0058 0.0052 0.0023 0.0066 0.0049 0.0048 0.0049 0.0239 0.0011 0.0047
Vacuole Periphery 0.0013 0.0004 0.0006 0.0006 0.001 0.0005 0.0032 0.0005 0.0006 0.0007 0.0002 0.0008 0.0005 0.0004 0.0004 0.0015 0.0002 0.0009

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 23.075 18.1273 11.5959 14.8571 17.2254 27.9542 21.0146 20.9019 14.6959 17.9928
Translational Efficiency 0.8271 1.148 0.8732 0.486 0.6108 0.3869 0.6468 0.3574 0.3497 0.5783

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1445 732 423 1478 1575 1588 315 249 3020 2320 738 1727

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 612.84 805.49 867.28 843.61 777.34 835.02 876.40 870.87 698.63 825.70 871.17 847.54
Standard Deviation 72.19 172.89 110.00 110.29 115.26 141.41 89.38 123.07 127.18 152.67 101.81 112.63
Intensity Change Log 2 — 0.394356 0.500987 0.461066 — 0.103265 0.173044 0.163912 — 0.238873 0.326864 0.302499

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000070 0.002990 0.000558 0.002854 0.000487 0.001198 0.000105 0.003208 0.000288 0.001764 0.000365 0.002905
Bud Neck 0.008771 0.010320 0.002952 0.009739 0.006841 0.019702 0.002420 0.009539 0.007764 0.016742 0.002725 0.009710
Bud Site 0.002046 0.031718 0.008766 0.036569 0.005675 0.024186 0.002405 0.045892 0.003938 0.026563 0.006051 0.037913
Cell Periphery 0.000324 0.000640 0.000280 0.000272 0.000528 0.000497 0.000211 0.000171 0.000431 0.000542 0.000250 0.000257
Cytoplasm 0.491529* 0.185072* 0.300204* 0.478736* 0.332103* 0.259819* 0.327987* 0.396000* 0.408385* 0.236235* 0.312063* 0.466807*
Cytoplasmic Foci 0.147019 0.216243* 0.020886 0.029858 0.152317 0.217056* 0.041896 0.054183 0.149782 0.216799* 0.029854 0.033365
Eisosomes 0.000220 0.000272 0.000084 0.000071 0.000459 0.000437 0.000121 0.000102 0.000345 0.000385 0.000100 0.000076
Endoplasmic Reticulum 0.013048 0.191330* 0.009606 0.005286 0.236684* 0.154145* 0.001814 0.002626 0.129679* 0.165878* 0.006280 0.004903
Endosome 0.005920 0.081877 0.005370 0.005726 0.021697 0.045504 0.003590 0.019599 0.014148 0.056980 0.004610 0.007727
Golgi 0.001768 0.036273 0.000123 0.003229 0.011482 0.022277 0.000144 0.003629 0.006834 0.026693 0.000132 0.003287
Lipid Particles 0.023502 0.040518 0.003534 0.002754 0.059225 0.047180 0.003194 0.002609 0.042133 0.045078 0.003389 0.002733
Mitochondria 0.002263 0.015945 0.000363 0.001732 0.006196 0.019899 0.000716 0.003641 0.004314 0.018651 0.000514 0.002007
Mitotic Spindle 0.000261 0.003868 0.000359 0.026023 0.001162 0.006186 0.005444 0.029394 0.000731 0.005455 0.002529 0.026509
None 0.006006 0.006466 0.004789 0.005106 0.003152 0.001516 0.006549 0.004242 0.004518 0.003078 0.005540 0.004981
Nuclear Periphery 0.002058 0.003600 0.002391 0.002572 0.004625 0.002652 0.001268 0.001240 0.003397 0.002951 0.001912 0.002380
Nuclear Periphery Foci 0.000954 0.014808 0.006251 0.003560 0.006168 0.004509 0.001369 0.002099 0.003673 0.007759 0.004167 0.003349
Nucleolus 0.001707 0.003844 0.000744 0.000596 0.000769 0.001179 0.000788 0.000541 0.001218 0.002020 0.000763 0.000588
Nucleus 0.264996* 0.024993 0.520073* 0.229040* 0.079345 0.043783 0.494530* 0.117970 0.168175* 0.037855 0.509171* 0.213026*
Peroxisomes 0.002265 0.007775 0.000475 0.001280 0.004243 0.008943 0.001842 0.002968 0.003297 0.008575 0.001059 0.001523
Vacuole 0.024544 0.100834 0.111917 0.152388 0.063821 0.109219 0.103302 0.296942* 0.045028 0.106574 0.108240 0.173230
Vacuole Periphery 0.000726 0.020614 0.000275 0.002611 0.003021 0.010110 0.000305 0.003404 0.001923 0.013424 0.000287 0.002725

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.52 -9.29 -2.10 1.91 -0.98 -2.89 3.62 -1.23 -0.52 -1.57 -5.64 -3.07 -2.05 0.66 -1.69
Bud Neck -1.04 4.83 -0.33 0.83 -6.41 -9.02 6.09 0.43 7.49 -3.03 -7.98 7.39 -1.10 6.65 -7.53
Bud Site -10.29 -2.56 -8.40 2.54 -4.27 -9.63 3.01 -3.08 0.01 -3.66 -14.59 -1.16 -8.20 1.51 -6.52
Cell Periphery -4.75 0.49 3.80 7.54 4.56 0.80 10.61 12.34 11.53 3.44 -3.17 6.79 10.05 12.26 4.24
Cytoplasm 29.24 17.85 10.92 -18.32 -9.49 8.00 2.97 0.41 -3.30 -1.64 24.87 13.53 3.44 -17.19 -9.71
Cytoplasmic Foci -8.43 28.95 29.67 26.83 -1.23 -9.92 23.10 20.45 29.11 -0.80 -13.33 37.05 39.66 45.40 0.77
Eisosomes -1.63 7.74 11.05 6.93 4.87 0.42 9.45 10.20 7.69 2.64 -1.08 11.60 13.82 9.88 7.05
Endoplasmic Reticulum -15.58 0.55 4.11 16.50 7.26 7.76 28.59 28.58 21.96 0.03 -4.70 25.14 25.93 27.22 5.34
Endosome -18.21 -0.69 3.28 18.80 4.42 -11.99 15.12 4.36 12.06 -2.42 -22.85 12.22 11.71 28.72 1.15
Golgi -11.75 6.32 0.95 11.82 -2.30 -7.07 15.22 9.91 13.93 -1.66 -14.50 16.07 9.23 18.52 -2.64
Lipid Particles -5.47 11.47 12.64 14.28 4.56 3.86 24.10 24.51 21.06 2.38 -1.33 25.70 26.70 25.34 4.76
Mitochondria -7.04 5.69 3.00 7.71 -3.82 -8.90 8.14 5.28 12.15 -1.35 -12.04 9.87 7.41 15.30 -3.46
Mitotic Spindle -5.36 -2.00 -7.49 -5.72 -7.30 -4.01 -1.05 -2.79 -1.79 -2.20 -5.50 -1.19 -7.79 -5.27 -6.78
None -0.22 2.52 2.33 1.32 -0.19 2.23 -0.94 -1.50 -3.04 -0.40 1.90 0.48 0.51 -1.42 -0.04
Nuclear Periphery -1.74 -2.03 -3.98 -0.03 -2.73 3.71 6.48 5.71 1.99 -0.94 1.01 3.51 0.01 -1.11 -4.39
Nuclear Periphery Foci -7.67 -8.51 -7.28 6.40 5.54 2.46 7.16 6.76 3.83 -1.24 -5.79 -2.88 1.74 6.73 4.05
Nucleolus -1.92 2.72 2.72 2.99 0.42 -2.66 1.31 1.93 3.75 0.96 -2.05 2.92 2.61 3.68 0.35
Nucleus 39.48* -15.35 -0.48 -37.19 14.90 10.56 -23.72 -5.45 -8.65 16.67 34.27 -28.36 -12.68 -36.93 19.66
Peroxisomes -6.04 8.23 6.83 7.91 -1.01 -7.39 3.33 4.90 9.98 -0.14 -10.32 6.09 11.35 15.76 0.14
Vacuole -13.74 -13.94 -31.84 -16.24 -6.22 -10.29 -8.37 -16.77 -14.44 -9.76 -18.20 -15.31 -32.62 -21.75 -9.25
Vacuole Periphery -12.69 2.74 -0.84 12.05 -1.93 -10.90 11.58 2.55 9.84 -1.51 -17.20 11.38 1.95 16.22 -2.26
Standard name
Human Ortholog
Description Alcohol acetyltransferase; responsible for the major part of volatile acetate ester production during fermentation; main enzyme involved in terpenyl acetate synthesis; potential roles in lipid and sterol metabolism
Localization
Cell Percentages ER (43%), cytoplasm (9%), mixed (31%)
Cell Cycle Regulation Yes
cytoplasm - MA (0.00025)
Subcompartmental Group N/A

Atf1

Atf1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Atf1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available